2021-05-04
ENA first public
2021-04-29
ENA last update
ERC000039
ENA-CHECKLIST
SAMEA8658910
External Id
Swiss Tropical and Public Health Institute
INSDC center alias
Swiss Tropical and Public Health Institute
INSDC center name
2021-05-04T00:42:32Z
INSDC first public
2021-04-29T21:12:17Z
INSDC last update
public
INSDC status
ERS6343456
SRA accession
NF54/PKAc cOE S4
Submitter Id
Swiss Tropical and Public Health Institute
collecting institution
Eva Hitz
collector name
malaria parasite P. falciparum
common name
Using a two-plasmid CRISPR/Cas9-based gene editing approach, a conditional PfPKAc overexpression (OE) cassette (PfPKAc-GFP-glms) has been inserted into the non-essential glp3 locus (Pf3D7_0709200, chromosome 7) in Plasmodium falciparum strain NF54 parasites. The transgenic line has been cloned out and two clonal lines have been obtained (NF54/PKAc cOE M1 and M2). PfPKAc-GFP OE can be induced via removal of glucosamine from the culture medium. Induction of PfPKAc-GFP OE is lethal in both NF54/PKAc cOE M1 and M2 clones. After constant selection of parasite growth in absence of glucosamine, six independent parasite populations tolerant to PfPKAc-GFP OE were obtained and these survivor populations have been termed NF54/PKAc-cOE S1-S3 (derived from NF54/PKAc cOE M1) and NF54/PKAc-cOE S4-S6 (derived from NF54/PKAc cOE M2).
description
intra-erythrocytic parasites
dev stage
Netherlands
geographic location (country and/or sea)
NF54
isolate
Plasmodium falciparum
http://purl.obolibrary.org/obo/NCBITaxon_5833
organism
Whole genome sequence of the transgenic NF54/PKAc cOE S4 survivor
title