characteristics.text: LIBEST_022796
characteristics.characteristic: External Id
characteristics.text: SAMN00165342
characteristics.characteristic: External Id
characteristics.text: North Dakota State University
characteristics.characteristic: INSDC center name
characteristics.text: 2010-03-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:55.197Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed using the SMART cDNA Library Construction Kit (DB-Bioscience-ClonTech, Palo Alto, CA) and the Gateway (Invitrogen, Carlsbad, CA) cloning system with modifications from manufacturer's protocols. Briefly, for first-strand cDNA synthesis, we used the attB2-oligo-dT primer (GGGGACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTTTVN) and attB1-Switch primer (GGGGACAAGTTTGTACAAAAAAGCAGGCTrGrGrG) for the first strand synthesis. The first strand was further amplified with primers attB1 (GGGGACAAGTTTGTACAAAAAAGCAGGCT) and attB2 (GGGGACCACTTTGTACAAGAAAGCTGGGT). The final products were cloned into the plasmid pDONR221 by recombination using BP clonase as described in the manufacturer's protocol (Invitrogen, Carlsbad, CA). The plasmids were transformed in DH10B E. coli strain.
characteristics.characteristic: description
characteristics.text: DH10B E. coli
characteristics.characteristic: lab-host
characteristics.text: Pseudocercospora fijiensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1873960
characteristics.characteristic: organism
characteristics.text: Mf-Hilo5
characteristics.characteristic: strain
characteristics.text: mycelia
characteristics.characteristic: tissue-type
characteristics.text: FL, mycelia grown in Flies medium at room temperature
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDONR221
characteristics.characteristic: vector