characteristics.text: LIBEST_021960
characteristics.characteristic: External Id
characteristics.text: SAMN00166292
characteristics.characteristic: External Id
characteristics.text: ESALQ - University of Sao Paulo
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:31.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This cDNA library was constructed from the phytopathogen F. graminearum isolated from spikes of brazilian wheat and grown under in vitro conditions. Mycelia from fungus was collected after growing for 8 days in PDA medium. Total RNA and mRNA were extracted according to detailed protocol presented by Sokolovsky et al. (1990) and Oligotex mRNA mini kit (Qiagen), respectively. The cDNA was constructed with the SuperScript Plasmid System for cDNA Synthesis and Cloning (Invitrogen), following manufacture's protocols. Plasmid DNA was purified using a modified alkaline lysis method. Sequencing reactions were conducted using the DYEnamic ET Terminator Cycle Sequencing Kit for ABI 3100 Genetic Analyzer (Applied Biosystem) According to the manufacturer's recommendations. Clones were sequenced by the 5' end with T7 primer. The quality and clustering of the ESTs were analyzed using the softwares Phred/Cap3.
characteristics.characteristic: description
characteristics.text: 8 days old
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain DH10B
characteristics.characteristic: lab-host
characteristics.text: Fusarium graminearum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5518
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Mycelia
characteristics.characteristic: tissue-type
characteristics.text: Fusarium graminearum grown under in vitro conditions
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector