characteristics.text: LIBEST_009984
characteristics.characteristic: External Id
characteristics.text: SAMN00164484
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:51.957Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sumai3
characteristics.characteristic: cultivar
characteristics.text: Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
characteristics.characteristic: description
characteristics.text: Adult plant
characteristics.characteristic: dev-stage
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Spike
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001278
characteristics.characteristic: tissue-type
characteristics.text: Wheat Fusarium graminearum infected spike cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK
characteristics.characteristic: vector