characteristics.text: SAMN00165252
characteristics.characteristic: External Id
characteristics.text: LIBEST_022703
characteristics.characteristic: External Id
characteristics.text: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:52.327Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Constructed using the pCMV-Script XR cDNA Library Construction Kit (Stratagene). polyA+ RNA was primered by Xho I-site tagged OligodT, and dCTP-Methylated cDNAs were synthesized. The cDNAs were linked to an EcoRI anchor and digested with Xho I. cDNAs larger than 0.9kb was recovered, ligated to the EcoRI and XhoI restricted plasmid pCMV-Script, and transformed to E. coli XL10-gold.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL10-gold
characteristics.characteristic: lab-host
characteristics.text: Dissostichus mawsoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_36200
characteristics.characteristic: organism
characteristics.text: ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: tissue-type
characteristics.text: Antarctic fish Dissostichus mawsoni adult ovary library
characteristics.characteristic: title
characteristics.text: pCMV-Script
characteristics.characteristic: vector