characteristics.text: LIBEST_022137
characteristics.characteristic: External Id
characteristics.text: SAMN00153718
characteristics.characteristic: External Id
characteristics.text: State University of Campinas
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.070Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: somatic
characteristics.characteristic: cell type
characteristics.text: Subtractive cDNA library. Total RNA was extracted from the dikaryotic saprotrophic mycelium of M. perniciosa grown for 14 days on minimal media containing either glucose (Glu) or cacao-pod extract (CP) as the only carbon source. For this subtracted library we generated two cDNA populations: CP (tester) and Glu (driver) using the Super SMART TM cDNA synthesis kit (BD Biosciences) and used in the subsequent steps of the suppressive subtractive hybridization procedure, according to the instructions of the BD Clontech TM PCR-Select TM Subtraction kit (BD Biosciences). The products of the secondary PCR were inserted into the pGEMÃ« TEasy plasmid vector (Promega Corporation, USA, Madison, WI) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: saprotrophic
characteristics.characteristic: dev-stage
characteristics.text: Theobroma cacao L.
characteristics.characteristic: lab-host
characteristics.text: Moniliophthora perniciosa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_153609
characteristics.characteristic: organism
characteristics.text: CP02
characteristics.characteristic: strain
characteristics.text: dikaryotic mycelium
characteristics.characteristic: tissue-type
characteristics.text: E-Sub(C-G)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEMTeasy (Promega)
characteristics.characteristic: vector