characteristics.text: SAMN00164606
characteristics.characteristic: External Id
characteristics.text: LIBEST_022370
characteristics.characteristic: External Id
characteristics.text: National Institute of Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:42.567Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S- FL3. Library was size selected for 1.0 kb, with a average insert size of 1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo, Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'.
characteristics.characteristic: description
characteristics.text: Monosiga ovata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_81526
characteristics.characteristic: organism
characteristics.text: DraIII
characteristics.characteristic: re_1
characteristics.text: DraIII
characteristics.characteristic: re_2
characteristics.text: 2A4
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002102
characteristics.characteristic: strain
characteristics.text: whole cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/GO_0005623
characteristics.characteristic: tissue-type
characteristics.text: Full length cDNA Library, Monosiga ovata, MOC
characteristics.characteristic: title
characteristics.text: pME18S-FL3
characteristics.characteristic: vector