characteristics.text: LIBEST_020446
characteristics.characteristic: External Id
characteristics.text: SAMN00154368
characteristics.characteristic: External Id
characteristics.text: Waksman Institute, Rutgers University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-10-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:49.070Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A. franciscana cDNAs were reversed transcribed from polyA-selected mRNAs. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5a.
characteristics.characteristic: description
characteristics.text: Mixed stages
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5alpha
characteristics.characteristic: lab-host
characteristics.text: Whole animal
characteristics.characteristic: organ
characteristics.text: Artemia franciscana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6661
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Mixed male and female
characteristics.characteristic: sex
characteristics.text: WSSP A. franciscana cDNA Library SfiIA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pTriplEx2
characteristics.characteristic: vector