characteristics.text: LIBEST_000390
characteristics.characteristic: External Id
characteristics.text: SAMN00155002
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1995-02-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:10.087Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis lesions from one patient was kindly provided by Dr. Kevin G. Becker (NINDS/NIH).
characteristics.characteristic: description
characteristics.text: Age 46
characteristics.characteristic: dev-stage
characteristics.text: DH10B (ampicillin resistant)
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: multiple sclerosis lesions
characteristics.characteristic: tissue-type
characteristics.text: Soares_multiple_sclerosis_2NbHMSP
characteristics.characteristic: title
characteristics.text: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid
characteristics.characteristic: vector