characteristics.text: SAMN00164118
characteristics.characteristic: External Id
characteristics.text: LIBEST_009607
characteristics.characteristic: External Id
characteristics.text: Yale University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.200Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: LRH library was constructed from cDNA of primary bone marrow cells depleted of lineage-committed cells and enriched for primitive cells by FACS sorting for cells with low level staining with rhodamine123 and Hoechst 33342 dyes. cDNA from 5000 cells derived from 30 mice, 5000 cells were were directionally cloned into SalI-EagI restriction site of the ( ZipLox (Gibco BRL). The ligated cDNA fragments were transformed into DH10B host cells. The original library had an initial plating complexity of 1.44X107 clones.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SalI site
characteristics.characteristic: re_1
characteristics.text: EagI site
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: BALB/c
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000602
characteristics.characteristic: strain
characteristics.text: Primary sorted bone marrow cells
characteristics.characteristic: tissue-type
characteristics.text: LRH (Lin Rhodamine and Hoechst dye)
characteristics.characteristic: title
characteristics.text: pZL1
characteristics.characteristic: vector