characteristics.text: SAMN00150950
characteristics.characteristic: External Id
characteristics.text: LIBGSS_000042
characteristics.characteristic: External Id
characteristics.text: University of Minnesota - Twin Cities
characteristics.characteristic: INSDC center name
characteristics.text: 1998-06-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:38:54.010Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: C. parvum (IOWA isolate) genomic DNA was sheared by gas driven nebulizer to produce fragments having a tight size distribution between 800 - 4000bp as described (http://www.genome.ou.edu). The randomly sheared gDNA was fractionated by electrophoresis. The DNA fragment with size between 800 - 1500bp was purified with QIAEX II kits (QIAGEN, Chatsworth, CA) and ligated into Sma I- and CIAP (Boehringer, Indianapolis, IN)-treated pBluescript SK(+/-) (Stratagene, La Jolla, CA) overnight at 16Co. Ligation products were transformed into E coli DH5a. Recombinant clones from the unamplified library were selected for sequence analysis using T3 and T7 primers.
characteristics.characteristic: description
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: Sma I
characteristics.characteristic: re_1
characteristics.text: Iowa isolate
characteristics.characteristic: strain
characteristics.text: Cryptosporidium parvum genomic random library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScript II SK(+/-)
characteristics.characteristic: vector