characteristics.text: LIBEST_006764
characteristics.characteristic: External Id
characteristics.text: SAMN00161299
characteristics.characteristic: External Id
characteristics.text: M. S. Swaminathan Research Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:15.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A one-year-old plant from the natural mangrove habitat in Pichavaram, Tamil Nadu, India was collected and brought to the M.S.Swaminatan Research Foundation. The whole plant was treated with the nutrient solution supplemented with 500 mM NaCl for 48 hours. Poly(A+)mRNA from the leaf tissue was purified and cDNA was prepared using Superscript Lambda System (Life Technologies, Cat. No. 19643-014). The cDNAs were size fractionated over SizeSep-400 spun column (Amersham-Pharmacia Biotech Cat No. 27-5105-01) and cloned in 5'Sal I - 3' Not I of pSPORT1 (Life Technologies Cat. No. 15383-011). The ESTs were sequenced from the 5' end using M13/pUC18 reverse primer in an Automated Sequencer (ABI310, Applied Biosystems) and submitted after editing to remove the vector and adapter sequences. BLAST search in BLASTX (Non-redundant)was carried out on 7th August, 2000 using default parameters and the results reported under putative identification/comment
characteristics.characteristic: description
characteristics.text: 1 year old plant
characteristics.characteristic: dev-stage
characteristics.text: Avicennia marina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_82927
characteristics.characteristic: organism
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Pichavaram
characteristics.characteristic: strain
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Avicennia marina leaf cDNA Library
characteristics.characteristic: title