characteristics.text: SAMN00160118
characteristics.characteristic: External Id
characteristics.text: LIBEST_005580
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:36.833Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-Oligo(dT) PCR-based library. Meloidogyne javanica cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. David Bird of North Carolina State University.
characteristics.characteristic: description
characteristics.text: enriched for eggs
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Meloidogyne javanica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6303
characteristics.characteristic: organism
characteristics.text: Meloidogyne javanica Egg SL1 Topo2 Kloek Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: Ampicillin and Kanamycin resistant
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector