characteristics.text: LIBEST_021945
characteristics.characteristic: External Id
characteristics.text: SAMN00153550
characteristics.characteristic: External Id
characteristics.text: Fujian Agriculture and Forestry University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:31.517Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: K326
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002067
characteristics.characteristic: cultivar
characteristics.text: The tobacco suppression subtractive hybridization cDNA library was constructed from tobacco cultivar k326. The root were collected at vigorous growing stage, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated using TRNzol-Reagent (Tangen), mRNA was extracted from total RNA by mRNA extraction kit (promega). cDNA synthesis and differentially expressed RNA was prepared following the protocol of CLONTECH PCR-SelectTM cDNA Subtraction kit. Then the subtracted cDNA collection was used to construct library directly with pGEM-T-Easy vector. The library was constructed by Dr. Jianqing Pan and Dr. Weijian Zhuang.
characteristics.characteristic: description
characteristics.text: vigorous growing stage
characteristics.characteristic: dev-stage
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: Roots
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000989
characteristics.characteristic: organ
characteristics.text: Nicotiana tabacum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4097
characteristics.characteristic: organism
characteristics.text: tobacco suppression subtractive hybridization cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T-Easy
characteristics.characteristic: vector