characteristics.text: LIBEST_015411
characteristics.characteristic: External Id
characteristics.text: SAMN00149808
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:04.317Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:14.287Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: First-strand cDNA was primed with the oligo-dT primer 5'-AATTCGAGCGGCCGCT(30)VN-3' and a switch primer 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3'. DNA was amplified by 26 cycles of PCR and then size selected for >0.5 kb. An aliquot was used to reamplify for 7 more cycles. The BstXI adaptor 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3' was ligated at both ends and non-directionally cloned into the BstXI site of the pcDNAIII vector. Original library contained 7.5 million clones. Library constructed by Mike Clarke (University of Michigan). Note: this is a NIH_MGC library.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000034
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: BstXI
characteristics.characteristic: re_1
characteristics.text: BstXI
characteristics.characteristic: re_2
characteristics.text: early hematopoietic precursor cells, isolated from bone marrow
characteristics.characteristic: tissue-type
characteristics.text: NIH_MGC_149
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pCDNAIII
characteristics.characteristic: vector