characteristics.text: LIBEST_021592
characteristics.characteristic: External Id
characteristics.text: SAMN00153298
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:21.787Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized from 4-week-old zebrafish gonad total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells. The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer (forward read) or by a anchored oligo-T primer (reverse read, _R).
characteristics.characteristic: description
characteristics.text: 4-week-old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: gonad
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000991
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Toh (Singaporean strain)
characteristics.characteristic: strain
characteristics.text: 4-week-old gonad (TLL)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBS-SK-Sfi
characteristics.characteristic: vector