characteristics.text: LIBEST_022646
characteristics.characteristic: External Id
characteristics.text: SAMN00164879
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-02-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:51.007Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequenced by DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: sexually induced female (mixed stages: 1.5, 10, 24, 48 hr after sexual induction)
characteristics.characteristic: dev-stage
characteristics.text: DH10B T1 resistant
characteristics.characteristic: lab-host
characteristics.text: Volvox carteri f. nagariensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3068
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Eve
characteristics.characteristic: strain
characteristics.text: CBOX Volvox carteri f. nagariensis induced female Eve, 2kb
characteristics.characteristic: title
characteristics.text: pCMV-SPORT6
characteristics.characteristic: vector