characteristics.text: SAMN00016999
characteristics.characteristic: External Id
characteristics.text: UNIL
characteristics.characteristic: INSDC center name
characteristics.text: 2012-02-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:57:22.133Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS084972
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS084972
characteristics.characteristic: SRA accession
characteristics.text: Total RNA was extracted from worker and queen pupae and adults (~175 total individuals) from 27 polygyne nests collected in Gainesville, Florida. RNA was extracted in three separate pools and subsequently combined into a single tube prior to mRNA purification. mRNA was isolated using the OligoDirect mini mRNA extraction kit following the manufacturer’s suggested protocol (Qiagen). Double stranded cDNA libraries were constructed using the SMART cDNA synthesis kit (Clontech) following the manufacturer’s suggested protocol. Approximately 500 nanograms of mRNA was used as starting material. Each of the four cDNA libraries were then normalized using the TRIMMER kit (Evrogen).
characteristics.characteristic: description
characteristics.text: Solenopsis invicta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13686
characteristics.characteristic: organism
characteristics.text: 175 Solenopsis invicta worker and queen pupae and adults from 27 polygyne nests, Florida
characteristics.characteristic: title