characteristics.text: LIBEST_011214
characteristics.characteristic: External Id
characteristics.text: SAMN00153895
characteristics.characteristic: External Id
characteristics.text: University of Manchester Institute of Science and Technology (UMIST)
characteristics.characteristic: INSDC center name
characteristics.text: 2002-11-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:25.813Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Modification of pBluescript II KS(+) [Stratagene] vector to accommodate cDNA produced with the T-trimmed protocol (Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3' end DNA sequencing by Glenn Fu, et al. U.S. Patent # 6,387,624). Cut pBluescript II KS(+) with NotI and EcoRI. Ligate in double stranded adaptor containing BsgI and BamHI sites [5'ggccgcgtgcagccccggatccgaaaaaaag] [5'aattctttttttcggatccggggctgcacgc]
characteristics.characteristic: description
characteristics.text: 20-21
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: organ
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Not1
characteristics.characteristic: re_2
characteristics.text: White Leghorn, Hisex
characteristics.characteristic: strain
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: CSEQCHL01
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resisitant)
characteristics.characteristic: v_type
characteristics.text: pBluescript II KS(+)
characteristics.characteristic: vector