characteristics.text: LIBEST_015931
characteristics.characteristic: External Id
characteristics.text: SAMN00154117
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2004-06-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:38.033Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Full length cDNA was synthesized from adult testis poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The plasmid library was normalized by using a reassociation-kinetics-based approach according to Bonaldo et al., (Genome Research 6: 791-806,1996) with minor modifications. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.
characteristics.characteristic: description
characteristics.text: adult (fully mature)
characteristics.characteristic: dev-stage
characteristics.text: pooled testis
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: AB
characteristics.characteristic: strain
characteristics.text: adult testis normalized (TLL)
characteristics.characteristic: title
characteristics.text: pBluescript II SK
characteristics.characteristic: vector