characteristics.text: LIBEST_000273
characteristics.characteristic: External Id
characteristics.text: SAMN00154887
characteristics.characteristic: External Id
characteristics.text: Institut fuer Genbiologische Forschung
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:07.510Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was isolated from epidermal fragments, in which the predominant living cells are guard cells. The cDNA library was constructed as per manufacturer's instructions (Stratagene, CA.). cDNA are directionally cloned, with the 5' end proximal to the EcoRI site and the 3' end proximal to the XhoI site. There is an additional NotI site downstream of the EcoRI site, because EcoRI/NotI linker adaptors (Pharmacia) were used in the construction of the library, instead of simply EcoRI adaptors.
characteristics.characteristic: description
characteristics.text: Solanum tuberosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4113
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Desiree
characteristics.characteristic: strain
characteristics.text: Epidermal Fragment cDNA Library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZapII
characteristics.characteristic: vector