characteristics.text: SAMN00154283
characteristics.characteristic: External Id
characteristics.text: LIBEST_018946
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:06.677Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized from 70 and 100 day old carp testis total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 100 day cDNA but not in the 70 day sample. The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T-Easy (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.
characteristics.characteristic: description
characteristics.text: 100 days post fertilization
characteristics.characteristic: dev-stage
characteristics.text: developing testis
characteristics.characteristic: organ
characteristics.text: Cyprinus carpio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7962
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: all-male (XY) offspring of XX females and androgenetic YY males
characteristics.characteristic: strain
characteristics.text: 100 day old common carp testis (subtracted)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T-Easy
characteristics.characteristic: vector