characteristics.text: SAMN00153096
characteristics.characteristic: External Id
characteristics.text: LIBEST_021373
characteristics.characteristic: External Id
characteristics.text: Institut de Recherche pour le Developpement (IRD)
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:16.763Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Gills
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002535
characteristics.characteristic: cell type
characteristics.text: Subtracted libraries were constructed with the PCR-select cDNA subtraction kit (Clontech). The subtraction was realised between mRNAs of Gills from fish exposed to hypersaline water and freshwater osmotic shock. The cDNA fragments obtained were cloned into the pCR2.1 vector (Invitrogen) and electroporated into DH10B cells.
characteristics.characteristic: description
characteristics.text: Juvenile
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000112
characteristics.characteristic: dev-stage
characteristics.text: Sarotherodon melanotheron
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_50613
characteristics.characteristic: organism
characteristics.text: subtracted libraries from gill of black-chinned tilapia exposed to hypersaline water and freshwater osmotic shock
characteristics.characteristic: title
characteristics.text: pCR(r)2.1-TOPO cloning vector from TOPO TA cloning kit (Invitrogen)
characteristics.characteristic: vector