characteristics.text: SAMN00004976
characteristics.characteristic: External Id
characteristics.text: Stanford University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2010-02-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:51:51.340Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS007076
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS007076
characteristics.characteristic: SRA accession
characteristics.text: Xylose positive segregants of SIHA Activ-Hefe 4 S. cerevisiae were crossed once to S288c and the resulting diploids were then sporulated. F2 segregants were scored for xylose utilization in a TECAN plate reader. 1.5mL of overnight YPD culture of each segregant grown was spun down, resuspended, and frozen in 300μL of sorbitol solution (0.9M sorbitol, 0.1M Tris pH 8, 0.1M EDTA). Samples were pooled by phenotype at this stage and genomic DNA was extracted as described (Treco, D.A., Preparation of Yeast DNA. Curr. Protoc. Mol. Biol. Vol. 5:13.11. 1989). The pool contained 16 positive segregants. 5μg of genomic DNA were prepared for sequencing using the Illumina Genomic DNA Sample Kit. Flow cells were prepared using the Illumina Standard Cluster Generation Kit v2, and samples were sequenced on the Illumina Genome Analyzer II. GAII data were analyzed with the Illumina 1.3.2 pipeline.
characteristics.characteristic: description
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Generic sample from Saccharomyces cerevisiae
characteristics.characteristic: title