characteristics.text: LIBEST_021913
characteristics.characteristic: External Id
characteristics.text: SAMN00154540
characteristics.characteristic: External Id
characteristics.text: Universite Laval
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:30.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Tissue: Dormant shoot tips dissected from buds. Bud scales and developing needles were removed, leaving a piece of tissue approximately 3 mm in length. Sample comprises shoot apex, leaf primordia as well as subtending primary stem tissues. Endodormant trees: after completion of second growth cycle, trees were transferred progressively from 20 deg C days/20 deg C nights to 20 deg C days/10 deg C nights for 3 weeks, 4 deg C days/4 deg C nights for 2 weeks, and then 2 deg C days/ deg C nights until sampling. Trees were at 2 deg C for 5.5 weeks prior to sampling. Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.
characteristics.characteristic: description
characteristics.text: Endodormant trees
characteristics.characteristic: dev-stage
characteristics.text: Xl1-Blue
characteristics.characteristic: lab-host
characteristics.text: Terminal bud
characteristics.characteristic: organ
characteristics.text: Picea glauca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3330
characteristics.characteristic: organism
characteristics.text: Sfi1-A
characteristics.characteristic: re_1
characteristics.text: Sfi1-B
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite (Monoecious)
characteristics.characteristic: sex
characteristics.text: Five individuals from an association population, representing families 370, 372, 663, 685, and 986
characteristics.characteristic: strain
characteristics.text: Dormant shoot tips dissected from buds
characteristics.characteristic: tissue-type
characteristics.text: GQ041 - Shoot tip - Dormant (Normalized)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAL17.3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector