characteristics.text: LIBEST_019685
characteristics.characteristic: External Id
characteristics.text: SAMN00150298
characteristics.characteristic: External Id
characteristics.text: Centre for Cellular and Molecular Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:39.933Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Robusta coffee Cultivar 'CxR'
characteristics.characteristic: cultivar
characteristics.text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit. The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry. Moisture-stressed leaf tissue obtained from Department of Plant Physiology, University of Agricultural Sciences, Bangalore, India. Robusta coffee Cultivar 'CxR' (an interspecific hybrid of Coffea congensis X Coffea canephora) developed at Central Coffee Research Institute, Chikamagalur, India
characteristics.characteristic: description
characteristics.text: Coffea congensis x Coffea canephora
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_213309
characteristics.characteristic: organism
characteristics.text: moisture-stressed leaf tissue
characteristics.characteristic: tissue-type
characteristics.text: Leaf EST Library of robusta coffee cultivar 'CxR'
characteristics.characteristic: title
characteristics.text: pMOSBlue Blunt Ended vector
characteristics.characteristic: vector