characteristics.text: LIBEST_002786
characteristics.characteristic: External Id
characteristics.text: SAMN00157344
characteristics.characteristic: External Id
characteristics.text: Sun Yat-sen University of Medical Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1999-12-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:16.673Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Epithelium adenocarcinoma
characteristics.characteristic: cell type
characteristics.text: LoVo
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006639
characteristics.characteristic: cell-line
characteristics.text: The human colon cancer LoVo cells were induced by 10-5 mol/L all-trans-retinoic acid and 10-7 mol/L 1,25-dihydroxy vitamin D3 for 2 days. Treated cell cDNA was used as driver and untreated cell cDNA as tester. Suppression subtractive hybridization (Clontech PCR-select cDNA subtraction kit) was used to screening the differentially exprssed genes. The differentially expressed sequences amplified were directly cloned into pT-Adv (Clontech advanTAge PCR cloning kit) and transformated into E. Coli DH5a cells. The positive clones were sequenced by using M13 reverse primer or T7 promoter primer.
characteristics.characteristic: description
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Colon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001155
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: LoVo express library before ATRA and D3 induced
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT-Adv
characteristics.characteristic: vector