characteristics.text: SAMN00014761
characteristics.characteristic: External Id
characteristics.text: Joint Genome Institute, U.S. Department of Energy
characteristics.characteristic: INSDC center name
characteristics.text: 2010-06-10T15:29:16.217Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-04-07T14:33:25.350Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS074581
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS074581
characteristics.characteristic: SRA accession
characteristics.text: Chlamydomonas reinhardtii, Mix-lowZnFeCuO2-NO3-MM
characteristics.characteristic: description
characteristics.text: Cells were incubated in the light in Zn-free or Fe-limited TAP, or TAP with NO3 instead of NH4, or in Cu-free minimal medium; for anaerobiosis, cells were incubated in sealed flasks in the dark in TAP
characteristics.characteristic: in vivo conditions
characteristics.text: Minus Co/Zn: C. reinhardtii strain CC-1021 cells were grown in modified TAP medium with no Co and no Zn at 24 Celsius, 180 RPM shaking, and under continuous light at 120 µmol/m^2*s. RNA was isolated from cells that were collected at a cell density of 2.9x10^6 cells/ml. Fe 0.25ÂµM: C. reinhardtii strain CC-1021 cells were grown in modified TAP medium with 0.25 ÂµM Fe-EDTA at 24 Celsius, 180 RPM shaking, and under continuous light at 90 Âµmol/m^2*s. Cells were transferred to 1ÂµM Fe medium twice prior to experimentation and inoculated in 0.25 ÂµM Fe medium at 1x10^4 cells/ml. RNA was isolated from cells collected at a cell density of 3.0x10^6 cells/ml. Fe 1ÂµM: C. reinhardtii strain CC-1021 cells were grown in modified TAP medium with 1 ÂµM Fe-EDTA at 24 Celsius, 180 RPM shaking, and under continuous light at 90 umol/m^2*s. Cells were transferred to 1ÂµM Fe medium twice prior to experimentation and inoculated in 1 ÂµM Fe medium at 1x10^4 cells/ml. RNA was isolated from cells collected at a cell density of 3.0x10^6 cells/ml. Dark 30 min: C. reinhardtii strain CC-124 cells were grown in TAP medium at 24 Celsius, 140 RPM shaking, and under continuous light at 80 Âµmol/m^2*s. Cells at 4x10^6 cells/ml were transferred to sealed bottles and grown in the dark. RNA was isolated from cells collected after 30 minutes. Dark 2 hrs: C. reinhardtii strain CC-124 cells were grown in TAP medium at 24 Celsius, 140 RPM shaking, and under continuous light at 80 Âµmol/m^2*s. Cells at 4x10^6 cells/ml were transferred to sealed bottles and grown in the dark. RNA was isolated from cells collected after 2 hours. Nitrogen: C. reinhardtii strain CC-1690 cells were grown in TAP medium or TAP -N +KNO3 at 24 Celsius, 140 RPM shaking, and under continuous light at 95 Âµmol/m^2*s. RNA was isolated from cells collected at a cell density of 3.0x10^6 cells/ml. RNA samples from the two nitrogen source samples were pooled to generate one sample. Minus Cu: C. reinhardtii strain CC-1021 cells were grown in modified TAP medium with no Cu at 24 Celsius, 200 RPM shaking, bubbled air, and under continuous light at 90 Âµmol/m^2*s. Cells were transferred to -Cu medium twice prior to experimentation and inoculated at 1x10^4 cells/ml. RNA was isolated from cells collected at a cell density of 3x10^6 cells/ml. The seven RNA samples were pooled in equal proportion for sequencing. Sample provided by Sabeeha Merchant, Anja Hemschemeier, Eugen Urzica, Janette Kropat, Aimee Terauchi and Madeli Castruita.
characteristics.characteristic: library description
characteristics.text: RAPID
characteristics.characteristic: library type
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: CC124(137c) and CC1021(2137)
characteristics.characteristic: strain
characteristics.text: Generic sample from Chlamydomonas reinhardtii
characteristics.characteristic: title