characteristics.text: SAMN00154969
characteristics.characteristic: External Id
characteristics.text: LIBEST_000357
characteristics.characteristic: External Id
characteristics.text: Departamento de Parasitologia, Microbiologia e Imunologia, Universidade de Sao Paulo (USP)
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.270Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA library of Schistosoma mansoni was provided by CLONTECH (Cambridge Bioscience, England). Poly (A)+ RNA was purified from male and female adult worms. The cDNA was constructed and cloned in lambda gt11 vector and method described by HUYNH, TU et al.(in DNA Cloning Techniques: A practical approach. D. Glover, ed. IRL Press. Oxford. 1985). Double-stranded cDNA were passed through a Sepharose 4B column to remove cDNA which are smaller than 600 bp. cDNAs in the range between 600 bp and 3.6 kb were cloned into the EcoRI site of lambda gt11.
characteristics.characteristic: description
characteristics.text: Y1088, Y1090
characteristics.characteristic: lab-host
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: LE
characteristics.characteristic: strain
characteristics.text: Schistosoma mansoni, Lambda gt11, Clontech
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda gt11
characteristics.characteristic: vector