characteristics.text: LIBEST_022169
characteristics.characteristic: External Id
characteristics.text: SAMN00165574
characteristics.characteristic: External Id
characteristics.text: Malaysian Palm Oil Board
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.800Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from oil palm embryoids using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryoid cDNAs were cloned and amplified once to produce a library with a titre of 2.93 X 10^9 pfu/ml.
characteristics.characteristic: description
characteristics.text: Proliferating Embryoid
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue_MRF'
characteristics.characteristic: lab-host
characteristics.text: Elaeis guineensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_51953
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Embryoid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0014374
characteristics.characteristic: tissue-type
characteristics.text: EN (Oil Palm Embryoid)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript_SK_plus
characteristics.characteristic: vector