characteristics.text: LIBEST_023372
characteristics.characteristic: External Id
characteristics.text: SAMN00165942
characteristics.characteristic: External Id
characteristics.text: University College Dublin
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:13.377Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Frontana
characteristics.characteristic: cultivar
characteristics.text: Germinating seeds of wheat cultivar Frontana were treated with water or DON (20ug ml-1) and harvested 24h post-treatment. 1mg of extracted RNA (Ansari et al. 2007) was used to construct a phagemid lTriplEx2 vector library using the SMARTTM cDNA Library Construction kit (Clontech). Escherichia coli strain BM25.8 was used to convert lTriplEx2 to pTriplEx2 plasmid clones. The library was normalized and plasmid DNA from normalized library clones transformed into E. coli strain DH10B.
characteristics.characteristic: description
characteristics.text: E. coli strain DH10B
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: tissue-type
characteristics.text: Triticum aestivum root cDNA library
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector