characteristics.text: LIBEST_001022
characteristics.characteristic: External Id
characteristics.text: SAMN00155611
characteristics.characteristic: External Id
characteristics.text: Cedars-Sinai Medical Center
characteristics.characteristic: INSDC center name
characteristics.text: 1997-10-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:20:52.067Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Lung root and neck lymph nodes from Caucasions 26-69 years old, plus liver and brain tissues from spontaneously aborted fetuses at gestational ages of 19-26 weeks. mRNAs from lymph nodes, fetal liver and fetal brain were mixed at equal concentrations. 5 ug of such mixed mRNAs was reverse transcribed with both oligo(dT) and random hexamer to synthesize double stranded cDNA. Blunt-ended ds-cDNAs were then ligated to an EcoR I (Not I) adaptor. Adaptor ligated cDNAs were then amplified by PCR using EN primer (5'> AATTCGCGGCCGCGTCGAC<3'). PCR amplified cDNAs were enriched for those specific for genomic DNAs from 16p13.3 by cDNA selection. Enriched cDNAs, ranging from 0.5-2.0 kb in size, were cloned into pAMP10 vector to construct the regional genomic DNAs specific cDNA library.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Regional genomic DNA specific cDNA library
characteristics.characteristic: title