characteristics.text: SAMN00164252
characteristics.characteristic: External Id
characteristics.text: LIBEST_009746
characteristics.characteristic: External Id
characteristics.text: Michigan State University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:45.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: USH20
characteristics.characteristic: cultivar
characteristics.text: The mRNA samples used for this library were derived from seedlings germinated for 4 days in 88mM H2O2. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from 4-day old seedlings germinated in moist filter paper. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBluescript XR vector (Stratagene).
characteristics.characteristic: description
characteristics.text: 4-day germination in 88mM H2O2
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: seeds
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000991
characteristics.characteristic: organ
characteristics.text: Beta vulgaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_161934
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: whole seedlings
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Sugar Beet peroxide germination cDNA library (subtracted)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II KS (+)
characteristics.characteristic: vector