characteristics.text: SAMN00164746
characteristics.characteristic: External Id
characteristics.text: LIBEST_022512
characteristics.characteristic: External Id
characteristics.text: Massachusetts Institute of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:46.857Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ps-n cells were harvested during late exponential to mid-stationary growth phase, under predominantly domoic-acid-producing conditions. First-strand cDNA was prepared from 5 B5g poly (A) + RNA using Superscript II, NC-p7, and oligo pd(TZ). Double-stranded cDNA was generated using RNase H, E. coli DNA polymerase I, and E. coli ligase. The ends of the cDNAs were polished with T4 DNA polymerase and GstXI adaptors were ligated to the cDNA ends. Ps-n cDNA was then fractionated on sucrose gradients, ligated into pMD1 (a pUC-based vector) and transformed, by electroporation, into E. coli DH10B cells (Das et al., 2001). T7 forward (TAATACGACTCACTATAGGG) and M13 reverse (CAGGAAACAGCTATGAC) flanked the cloning site of the pMD1 vector.
characteristics.characteristic: description
characteristics.text: Ps-n clone CL-125
characteristics.characteristic: isolate
characteristics.text: Pseudo-nitzschia multiseries
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_37319
characteristics.characteristic: organism
characteristics.text: Ps-n cDNA Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMD1
characteristics.characteristic: vector