characteristics.text: LIBGSS_002985
characteristics.characteristic: External Id
characteristics.text: SAMN00151972
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:39:11.593Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Faribault
characteristics.characteristic: cultivar
characteristics.text: BAC subclones were prepared from selected RGA-positive BACs within the UMN BAC library (Danesh et al., Theor. Appl. Genet. 96:196, 1998) which was constructed using the EcoRI site of pECSBAC4. The library consists of 72,960 clones with an average insert size of 120 kb, equal to 7 haploid genome equivalents. Screening of the library was done by hybridization of high-density colony filters with radiolabeled soybean RGA probes of the nonTIR-NBS-LRR class. Four high density filters, each containing 18,432 clones (doubly spotted), represent the whole library for colony screening. Selected RGA-positive BACs were used as template for degenerate PCR amplification using primers corresponding to the P-loop and the RNBS-D motifs of nucleotide-binding sites (NBS) of nonTIR-NBS-LRR resistance genes. The PCR products were cloned into plasmids with the pGEM-T Easy vector system (Promega, Madison WI).
characteristics.characteristic: description
characteristics.text: cotyledon stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007049
characteristics.characteristic: dev-stage
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: cotyledon leaves
characteristics.characteristic: tissue-type
characteristics.text: BAC subclone sequences from soybean BACs containing resistance gene analogs (RGAs)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T Easy vector
characteristics.characteristic: vector