characteristics.text: SAMN00155515
characteristics.characteristic: External Id
characteristics.text: LIBEST_000917
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1997-07-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:26.187Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mature bradyzoites were obtained from infected mouse brains by percoll density centrifugation. The original library was constructed by Steve Parmley, Palo Alto Medical Foundation. cDNAs were synthesized by priming with oligo d(T) and directionally cloned into the EcoRI/NotI sites of lambda gt11. Warning: the library contains a small percentage of host cDNAs derived from mouse cells. Inserts from this cDNA library were excised with Not1 and EcoR1, size selected in a range of 0.7 - 2.0 kb and subcloned into Bluescript II SK- (Adrian Hehl, Ian Manger and John Boothroyd, Stanford University)
characteristics.characteristic: description
characteristics.text: Bradyzoite
characteristics.characteristic: dev-stage
characteristics.text: DH10
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: ME49
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006645
characteristics.characteristic: strain
characteristics.text: TgME49 invivo Bradyzoite cDNA size selected
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Bluescript II SK-
characteristics.characteristic: vector