characteristics.text: LIBEST_015936
characteristics.characteristic: External Id
characteristics.text: SAMN00154122
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2004-06-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:38.147Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ORESTES libraries were generated according to Neto et al. (Gene 186: 135-142, 1997). mRNA isolated from adult testis was reverse transcribed and PCR amplified using oligonucleotide primers designed for AFLP analysis or for amplification of specific genes from Arabidopsis, yeast or rice. The amplification products with a smear or multiple bands (> 500 bp) were then cloned into pGEM-T vector (Promega) and transformed into XL-10 Gold competent cell (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.
characteristics.characteristic: description
characteristics.text: adult (fully mature)
characteristics.characteristic: dev-stage
characteristics.text: pooled testis
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Toh (Singaporean strain)
characteristics.characteristic: strain
characteristics.text: adult testis ORESTES (TLL)
characteristics.characteristic: title
characteristics.text: pGEM-T
characteristics.characteristic: vector