characteristics.text: SAMN00152718
characteristics.characteristic: External Id
characteristics.text: LIBEST_020962
characteristics.characteristic: External Id
characteristics.text: UC Davis, Department of Pomology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.273Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Paradox clone 'PX1'
characteristics.characteristic: cultivar
characteristics.text: Five micropropagated plants of the walnut Paradox clone PX1 were grown in the greenhouse. On May 19, 2006 the roots of these plants were harvested, frozen in liquid nitrogen and stored at -80C. RNA was isolated from individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit) from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen). Individual colonies were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).
characteristics.characteristic: description
characteristics.text: Small greenhouse plants
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10-Beta
characteristics.characteristic: lab-host
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Juglans hindsii x Juglans regia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_432290
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: WRO-02: Walnut root
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector