characteristics.text: SAMN00163419
characteristics.characteristic: External Id
characteristics.text: LIBEST_008905
characteristics.characteristic: External Id
characteristics.text: Harvard University, Howard Hughes Medical Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:20.310Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was prepared by Catherine S. Lee and has not been published. The pancreas was obtained from Gerard Gradwohl (PNAS 97 P1607-1611, 2000). The cDNA's were prepared with an oligo containing a NotI site, and SalI linkers were added to the ends. The inserts were cut with NotI before being cloned into the NotI-SalI sites in the vectors. This is one of two libraries, ngn3 wt and ngn3 -/-. The wt library is in pSPORT1, T7 promoter is 5'.
characteristics.characteristic: description
characteristics.text: p.c. 14.5
characteristics.characteristic: dev-stage
characteristics.text: E. coli-DH12S (GIBCO)
characteristics.characteristic: lab-host
characteristics.text: pancreas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001264
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Sal I
characteristics.characteristic: re_2
characteristics.text: 129/Sv x CD1
characteristics.characteristic: strain
characteristics.text: Kaestner ngn3 wt
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1 (GIBCO)
characteristics.characteristic: vector