characteristics.text: SAMN00166329
characteristics.characteristic: External Id
characteristics.text: LIBEST_023436
characteristics.characteristic: External Id
characteristics.text: Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:15.010Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Golden
characteristics.characteristic: cultivar
characteristics.text: Papaya fruit were harvested at color break to 25% yellow. Ripening was induced by exogenous ethylene: fruit were left in a 240 dm3 chamber and exposed to a concentration of 100 ppm ethylene in a constant flow-through system during 2 h, and 10 h in a closed system. After treatment, fruit were ventilated and left to ripe under controlled temperature and humidity. Fruit before ethylene treatment were taken as the sample of unripe papayas. Fruit that were at the peak of respiration and low pulp firmness were the sample of climacteric ripe papayas. mRNA Differential display was performed using a total of 90 primer pairs combinations according to the instructions on the Delta Differential Display Kit (Clontech Lab.).
characteristics.characteristic: description
characteristics.text: 150 days after anthesis
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Carica papaya
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3649
characteristics.characteristic: organism
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Pulp
characteristics.characteristic: tissue-type
characteristics.text: Induced ripening papaya pulp
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR4-TOPO
characteristics.characteristic: vector