characteristics.text: SAMN00157400
characteristics.characteristic: External Id
characteristics.text: LIBEST_002843
characteristics.characteristic: External Id
characteristics.text: Sun Yat-sen University of Medical Scicences
characteristics.characteristic: INSDC center name
characteristics.text: 2000-01-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:17.880Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cardiac muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002349
characteristics.characteristic: cell type
characteristics.text: The cDNA libraries were constructed using fetal heart in different growth stages and adult heart as materials, respectively. The cDNA probes-labelled from the total RNA of fetal heart hybridized with recombinant plasmid-denatured from the cDNA library of adult heart at the ratio of 1 to 10, which was repeated three times for enriched specific probes. Positive clones were screened by the way that the enriched specific probes hybridized with the given colonies of fetal heart cDNA library and sequenced by using T7 promoter primer.
characteristics.characteristic: description
characteristics.text: embryonic day 96-140 post-fertilization
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH5a
characteristics.characteristic: lab-host
characteristics.text: heart
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000948
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: BamH1
characteristics.characteristic: re_1
characteristics.text: EcoR1
characteristics.characteristic: re_2
characteristics.text: Human fetal heart cDNA library (Liu,L. and Gu,J.)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT 1
characteristics.characteristic: vector