characteristics.text: LIBEST_019946
characteristics.characteristic: External Id
characteristics.text: SAMN00154336
characteristics.characteristic: External Id
characteristics.text: Instituto Butantan
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:32.690Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 4ug of mRNA from Amblyomma cajennense tick salivary glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).
characteristics.characteristic: description
characteristics.text: Salivary glands
characteristics.characteristic: organ
characteristics.text: Amblyomma cajennense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_34607
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: Salivary glands
characteristics.characteristic: tissue-type
characteristics.text: Amblyomma cajennense tick library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM11Zf+
characteristics.characteristic: vector