characteristics.text: SAMN00154874
characteristics.characteristic: External Id
characteristics.text: LIBEST_000260
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1995-02-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:07.167Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Toxoplasma RH strain tachyzoites were grown in human foreskin fibroblast cultures in vitro. The library was constructed by K.L. Wan, Cambridge University. cDNAs were synthesized from polyA RNAs by oligo d(T) priming and directionally cloned into the EcoRI to XhoI sites of the Lambda ZapII vector using the ZAP-cDNA synthesis kit (Stratagene). WARNING: the library contains a small percentage of cDNAs derived from the human host cells.
characteristics.characteristic: description
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: RH
characteristics.characteristic: strain
characteristics.text: TgRH Tachyzoite cDNA
characteristics.characteristic: title
characteristics.text: Lambda ZAP
characteristics.characteristic: vector