characteristics.text: LIBEST_004133
characteristics.characteristic: External Id
characteristics.text: SAMN00158684
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-05-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:51.270Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from fully expanded leaves of greenhouse grown plants that were 2 weeks old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli Electro- Max DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana- Champaign. email: l-vodkin@uiuc.edu
characteristics.characteristic: description
characteristics.text: 2 week old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: fully expanded leaves of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1037
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector