characteristics.text: SAMN00154084
characteristics.characteristic: External Id
characteristics.text: LIBEST_015612
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2004-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:28.500Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PG-29
characteristics.characteristic: cultivar
characteristics.text: mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.
characteristics.characteristic: description
characteristics.text: E. coli DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Foliage from 25 year old trees harvested at Kalamalka Research Station in Vernon, British Columbia in 2001
characteristics.characteristic: organ
characteristics.text: Picea glauca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3330
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Flushing buds harvested May 16th, young shoots harvested June 15th and mature shoots harvested June 15th
characteristics.characteristic: tissue-type
characteristics.text: WS-PS-A-2
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector