characteristics.text: LIBEST_017201
characteristics.characteristic: External Id
characteristics.text: SAMN00150151
characteristics.characteristic: External Id
characteristics.text: Ludwig-Maximilians-Universitat (LMU)
characteristics.characteristic: INSDC center name
characteristics.text: 2005-05-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:33.083Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B-129 Gifu
characteristics.characteristic: cultivar
characteristics.text: 50 ug of total RNA prepared from L. japonicus roots were used for PolyA+ extraction on Dyna beads (Dynal, Norway) according to the manufacturer's instructions, which was subjected to cDNA-synthesis. The cDNA-AFLP analysis was performed as described (Durrant, W.E., Rowland, O., Piedras, P., Hammond-Kosack, K.E., and Jones, J.D. (2000). cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. Plant Cell 12, 963-977). The [33P]-labelled cDNA-fragments were separated on 6% PAA-gels and visualized by autoradiography. Differential cDNA-AFLP fragments were cut out of the gel and reamplified. DNA sequences of reamplified cDNA-AFLP fragments were determined by either direct sequencing or after cloning into pGEMT-EASY.
characteristics.characteristic: description
characteristics.text: root
characteristics.characteristic: organ
characteristics.text: Lotus japonicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_34305
characteristics.characteristic: organism
characteristics.text: cDNA-AFLP fragments from Lotus japonicus roots
characteristics.characteristic: title