characteristics.text: LIBEST_020951
characteristics.characteristic: External Id
characteristics.text: SAMN00152709
characteristics.characteristic: External Id
characteristics.text: Cinvestav Irapuato
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:04.937Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Serrano Tampiqueno 74
characteristics.characteristic: cultivar
characteristics.text: Arabidopsis thaliana cv. Columbia plants and Capsicum annuum cv. Serrano Tampiqueno 74 plants were grown in a growth chamber at 27ÂoC Â± 1ÂoC with a photoperiod 18 h light/6 h. The cDNA library was made from RNA extracted from pooled flowers including from bud to postanthesis stages of each plant. Suppression subtraction hybridization was performed following the instructions of the PCR-Select cDNA subtraction kit (Clontech 637401 Mountain View, CA). We utilized a SSH protocol to subtract driver cDNAs (A. thaliana) from tester cDNAs (C. annuum) in order to obtain those cDNAs unique to the tester cDNA population. 2.5 micrograms each of tester and driver mRNA were used to proceed with the subtraction. The subtracted product was used to construct a cDNA library. PCR products obtained by the second hybridization were inserted into pJET1 (Fermentas Vilnius, Lt.).
characteristics.characteristic: description
characteristics.text: pooled flowers including from bud to postanthesis stages
characteristics.characteristic: dev-stage
characteristics.text: flower
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009046
characteristics.characteristic: organ
characteristics.text: Capsicum annuum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4072
characteristics.characteristic: organism
characteristics.text: SSH library of flowers of Capsicum - Arabidopsis
characteristics.characteristic: title
characteristics.text: pJET1
characteristics.characteristic: vector