characteristics.text: LIBEST_014167
characteristics.characteristic: External Id
characteristics.text: SAMN00154017
characteristics.characteristic: External Id
characteristics.text: UC Davis, Plant Pathology
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:47.480Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cabernet Sauvignon
characteristics.characteristic: cultivar
characteristics.text: CAB2 is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 berries. Samples were collected at full bloom (80 to 100% flowers showing dehiscence of calyptras or caps and anthers fully extended). Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.
characteristics.characteristic: description
characteristics.text: Bloom
characteristics.characteristic: dev-stage
characteristics.text: Flower - Bloom
characteristics.characteristic: organ
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Cabernet Sauvignon Flower bloom - CAB2
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR
characteristics.characteristic: vector