characteristics.text: LIBEST_022553
characteristics.characteristic: External Id
characteristics.text: SAMN00164788
characteristics.characteristic: External Id
characteristics.text: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:47.860Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Barke
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0004518
characteristics.characteristic: cultivar
characteristics.text: developing caryopsis, 8-15 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
characteristics.characteristic: description
characteristics.text: 8-15 DAP (days after pollination)
characteristics.characteristic: dev-stage
characteristics.text: XL10-Gold
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: EcoRI (5'-end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3'-end of cDNA)
characteristics.characteristic: re_2
characteristics.text: developing caryopsis
characteristics.characteristic: tissue-type
characteristics.text: HB (Hordeum vulgare cv. Barke)
characteristics.characteristic: title
characteristics.text: pBluescript SK+
characteristics.characteristic: vector