characteristics.text: SAMN00153587
characteristics.characteristic: External Id
characteristics.text: LIBEST_021989
characteristics.characteristic: External Id
characteristics.text: University of California at Davis (UCD)
characteristics.characteristic: INSDC center name
characteristics.text: 2007-10-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:32.737Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the seven-day old seedling of Arabidopsis Col-0 plants using the TRIzol procedure (Invitrogen, Carlsbad, CA). Template mRNA was then purified from total RNA using Dynabeads Oligo (dT)25 (Dynal Biotech, Lake Success, NY, USA). RACE-PCR products were cloned into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced.
characteristics.characteristic: description
characteristics.text: seven-day old seedling
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: strain
characteristics.text: Arabidopsis thaliana Col-0 RACE seven-day old seedling
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1-TOPO vector
characteristics.characteristic: vector