characteristics.text: LIBGSS_000123
characteristics.characteristic: External Id
characteristics.text: SAMN00151907
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 1999-06-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T13:20:00.943Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Constructed for The Institute for Genomic Research by Bohui Zhao in Pieter de Jong's laboratory (Roswell Park Cancer Institute, Buffalo, NY). Briefly, Trypanosoma brucei TREU927/4 GUTat 10.1 agarose embedded DNA was partially digested with a combination of Eco RI and Eco RI methylase (RPCI93-EcoRI segment) or Dpn II (RPCI93-DpnII segment). High molecular weight fragments were ligated in pBACe3.6 vector digested with Eco RI or Bam H1, respectively. The average insert size is 141 Kb. Total coverage (both segments): > 90 X the haploid non-minichromosomal genome.
characteristics.characteristic: description
characteristics.text: Trypanosoma brucei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5691
characteristics.characteristic: organism
characteristics.text: Bam HI
characteristics.characteristic: re_1
characteristics.text: Bam HI
characteristics.characteristic: re_2
characteristics.text: TREU927/4 GUTat 10.1
characteristics.characteristic: strain
characteristics.text: RPCI93-DpnII
characteristics.characteristic: title
characteristics.text: BAC
characteristics.characteristic: v_type
characteristics.text: pBACe3.6
characteristics.characteristic: vector