characteristics.text: LIBEST_009586
characteristics.characteristic: External Id
characteristics.text: SAMN00164097
characteristics.characteristic: External Id
characteristics.text: Southern Illinois University at Carbondale
characteristics.characteristic: INSDC center name
characteristics.text: 2001-06-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:41.490Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Forrest
characteristics.characteristic: cultivar
characteristics.text: Forrest roots were inoculated with Fusarium solani f. sp. glycinae and samples were collected after 14 days of inoculation. Total RNA was used for cDNA synthesis using SMART PCR cDNA synthesis Kit (CLONTECH, CA). CLONTECH PCR-Select Subtraction system was used to enrich rare transcripts expressed in Forrest roots under inoculation by F. solani and cloned in pGEM vector. ESTs were sequenced using M13 universal forward or reverse primer.
characteristics.characteristic: description
characteristics.text: 14 days after inoculation
characteristics.characteristic: dev-stage
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: Forrest infected Subtraction Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM
characteristics.characteristic: vector