characteristics.text: LIBEST_022614
characteristics.characteristic: External Id
characteristics.text: SAMN00164847
characteristics.characteristic: External Id
characteristics.text: University of Georgia
characteristics.characteristic: INSDC center name
characteristics.text: 2008-02-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:50.140Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Larry Rosen, S1 and S2 population
characteristics.characteristic: cultivar
characteristics.text: The library was prepared from polyA+ RNA from premeiotic flower buds. Only floral buds with diameter of 1.5 mm or less were harvested for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCGGCGTAG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1715; Primary Titer: 2.7E6 pfu total; Amplified Titer: 2E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was generated by the Ancestral Angiosperm Project (www.AncestralAngiosperm.org) funded by NSF's Plant Genome Research Program (DBI-0638595).
characteristics.characteristic: description
characteristics.text: premeiotic
characteristics.characteristic: dev-stage
characteristics.text: SOLR (E. Coli)
characteristics.characteristic: lab-host
characteristics.text: Premeiotic flower buds
characteristics.characteristic: organ
characteristics.text: Aristolochia fimbriata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_158543
characteristics.characteristic: organism
characteristics.text: Xho1
characteristics.characteristic: re_1
characteristics.text: EcoR1
characteristics.characteristic: re_2
characteristics.text: Flower buds
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000056
characteristics.characteristic: tissue-type
characteristics.text: Aristolochia fimbriata flower bud library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScript_SKminus
characteristics.characteristic: vector