characteristics.text: LIBEST_021606
characteristics.characteristic: External Id
characteristics.text: SAMN00153312
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:22.167Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Zebrafish transgenic to vas::egfp were sorted based on their gonadal EGFP levels as transforming males and juvenile females at the age of 5 weeks post fertilization (Wang et al., 2007, J. Fish Biol. 70: 33-44). cDNA was synthesized from 5-week old transforming male gonad and 5-week-old ovary total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 5-week old transforming male gonad but not in the 5-week-old ovary sample. The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.
characteristics.characteristic: description
characteristics.text: 5-week-old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: gonad
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000991
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: mainly males
characteristics.characteristic: sex
characteristics.text: Toh (Singaporean strain)
characteristics.characteristic: strain
characteristics.text: 5-week-old transforming male gonad subtracted against 5-week-old ovary (TLL)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector