characteristics.text: SAMN00152170
characteristics.characteristic: External Id
characteristics.text: LIBEST_018552
characteristics.characteristic: External Id
characteristics.text: The Jackson Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:55.390Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: oocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000023
characteristics.characteristic: cell type
characteristics.text: Cloned unidirectionally from mRNA prepared from fully-grown germinal vesicle stage oocytes isolated 46 hours after PMSG injection. NotI(dT) primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. SalI linker: 5'-TCGACCCACGCGTCCG-3'. cDNAs were size-selected for 500 bp and cloned into the SalI/NotI sites of pSPORT vector (Life Technologies). Average insert size: 1.5 kb. I.M.A.G.E. library ID 1182.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SalI (5')
characteristics.characteristic: re_1
characteristics.text: NotI (3')
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Eppig Hampl Solter fully-grown mouse oocyte
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector