characteristics.text: SAMN00155592
characteristics.characteristic: External Id
characteristics.text: LIBEST_001001
characteristics.characteristic: External Id
characteristics.text: Smith College Department of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1997-09-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:28.323Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Brugia malayi is a lymphatic filarial nematode parasite of humans. mRNA was prepared from third stage larvae of Brugia malayi isolated from the peritoneal cavity of jirds nine days after infection. The mRNA was converted to double stranded cDNA using reverse transcriptase and oligo (dT) followed by Rnase H and DNApol I. The library was constructed by Michelle Lizotte-Waniewski. The library is available from the Filarial Genome Project Resource Center: contact Dr. S.A. Williams, Clark Science Center, Smith College, Northampton, MA 01063 USA phone +1 413 585-3826 fax +1 413 585-3786 email genome@smith.edu
characteristics.characteristic: description
characteristics.text: third stage larvae, nine days after infection
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Eco RI (5' end)
characteristics.characteristic: re_1
characteristics.text: Xho I (3' end)
characteristics.characteristic: re_2
characteristics.text: Brugia malayi L3 molting-day 9 larva cDNA (SAW97MLW-BmL3d9)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: LambdaZap II (UniZAP XR)
characteristics.characteristic: vector