characteristics.text: LIBEST_004012
characteristics.characteristic: External Id
characteristics.text: SAMN00158565
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:48.453Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: unfertilized egg
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002443
characteristics.characteristic: cell type
characteristics.text: PolyA-selected mRNA was prepared from unfertilized Xenopus laevis eggs. The library was constructed in the vector pT7T3-Pac as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. 'Normalization and subtraction: two approaches to facilitate gene discovery', Genome Research 6:791-806, 1996. The first strand synthesis used a NotI-dT18 primer; double stranded cDNAs were ligated to EcoRI adapters, digested with NotI, and directionally cloned into the NotI and EcoRI-digested pT7T3-Pac vector. The library contained approximately 7.2 X 10^5 recombinants, with average insert sizes of 1-1.5 kb.
characteristics.characteristic: description
characteristics.text: unfertilized egg
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002443
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: unfertilized egg
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002443
characteristics.characteristic: tissue-type
characteristics.text: Blackshear/Soares normalized Xenopus egg library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector