characteristics.text: LIBEST_001245
characteristics.characteristic: External Id
characteristics.text: SAMN00155831
characteristics.characteristic: External Id
characteristics.text: Smith College Department of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1998-04-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:35.603Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Lymphatic filarial nematode parasite of humans. cDNA inserts were obtained by subtracting the JHU93SL-BmL3 cDNA library (constructed using SL sequence and PCR) from SAW94WL-BmL3 cDNA library (constructed using conventional cDNA methods). cDNA was amplified from both libraries using PCR and SL cDNA clones were eliminated through hybridization. The library has 5 x 10E6 independent recombinants and the average insert size is ~ 600 bp. The library was constructed by Ying Gong. The library is available from Dr.S.A.Williams, email: genome@smith.edu.
characteristics.characteristic: description
characteristics.text: conventional L3 cDNA minus spliced-leader L3 cDNA
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Brugia malayi L3 subtracted cDNA library (SAW97YG-BmL3SB)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP II (Stratagene)
characteristics.characteristic: vector