characteristics.text: LIBEST_004371
characteristics.characteristic: External Id
characteristics.text: SAMN00158921
characteristics.characteristic: External Id
characteristics.text: University of North Carolina Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 2000-05-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:57.067Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This subtracted cDNA library was constructed by inserting desired differentially expressed sequences into a plasmid pGEM-T, followed by transforming E. coli strain JM109. Differentially expressed sequences from fasted rat liver were generated by suppression subtractive hybridization, in which mRNA from fasted rats was used as a test and mRNA from control fed rats as a reference. A standard tailing procedure with Taq DNA polymerase was employed to introduce 3'-A overhang to the desired cDNAs before cloning into pGEM-T. Subtraction efficiency of this library was shown to be >60%. The average insert size of clones in this library is 369 bp (20 bp).
characteristics.characteristic: description
characteristics.text: five-week-old postnatal, fasting for 48 hours
characteristics.characteristic: dev-stage
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sprague-Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: Rat Liver Subtracted cDNA Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector