characteristics.text: SAMN00153902
characteristics.characteristic: External Id
characteristics.text: LIBEST_011916
characteristics.characteristic: External Id
characteristics.text: 2002-09-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:43.613Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: NCI_CGAP_FL0 is a cDNA library derived from a pool of mRNA obtained from 4 cell lines from grade III chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAGGTCGGTG. The cell line was provided by Dr James Martin from University of Iowa.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Chondrosarcoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000333
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: Cell lines
characteristics.characteristic: tissue-type
characteristics.text: NCI_CGAP_FL0
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT7T3-Pac (Pharmacia) with a modified polylinker
characteristics.characteristic: vector