characteristics.text: LIBEST_020934
characteristics.characteristic: External Id
characteristics.text: SAMN00152692
characteristics.characteristic: External Id
characteristics.text: Northwest A & F University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:04.560Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: young leaves
characteristics.characteristic: cell type
characteristics.text: Total RNA was extracted by Trizol, then reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech company) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.
characteristics.characteristic: description
characteristics.text: stage at 4-5 leaves, after 2 days inoculation with Stripe rust pathogen 32
characteristics.characteristic: dev-stage
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: SSH-cDNA Library of stripe rust infection in leaves of stripe rust resistant Shaanmai 139 compared with no infection
characteristics.characteristic: title