characteristics.text: LIBEST_003742
characteristics.characteristic: External Id
characteristics.text: SAMN00158298
characteristics.characteristic: External Id
characteristics.text: 2000-02-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:41.030Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The NIH_BMAP_M_S4 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S4, NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S4) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with a pool of the NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 libraries in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S4 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)
characteristics.characteristic: description
characteristics.text: 27-32 days
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: NIH_BMAP_M_S4
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector