characteristics.text: SAMN00155071
characteristics.characteristic: External Id
characteristics.text: LIBEST_000461
characteristics.characteristic: External Id
characteristics.text: Charite University Hospital, Humboldt University of Berlin
characteristics.characteristic: INSDC center name
characteristics.text: 1996-07-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:11.913Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: monocytes
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000576
characteristics.characteristic: cell type
characteristics.text: Monocytes were prepared from blood by ficoll-hypaque, percoll and T cell rosetting purification steps (purity: 96 %). mRNA was prepared from activated monocytes from a patient with rheumatoid arthritis. mRNA was reverse transcribed with MuLV. Using Eco-RI linkers cDNA was cloned into l-gt-11 vector arms. The cDNA library was screened by differential hybidization using radioactively marked ss-cDNA from activated and non-activated monocytes.
characteristics.characteristic: description
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Eco-RI
characteristics.characteristic: re_1
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: RA-MO-III
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: l-gt-11
characteristics.characteristic: vector