characteristics.text: SAMN00165907
characteristics.characteristic: External Id
characteristics.text: LIBEST_023332
characteristics.characteristic: External Id
characteristics.text: Advanced Technologies (Cambridge)
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:11.407Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Plants were grown on soil in glasshouses under long days (16h:8h). RNA was extracted from trichomes based on the method of Aziz et al. (2004). Briefly, stems from 30 plants were cut into 5-10cm fragments and flash frozen in liquid nitrogen. Segments were individually shaken in pre-frozen 50ml platic tubes to release the trichomes. Collected trichomes were stored in Tri Reagent (Sigma) and RNA was extracted according to manufacturers instructions.
characteristics.characteristic: description
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Nicotiana tabacum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4097
characteristics.characteristic: organism
characteristics.text: Samsun
characteristics.characteristic: strain
characteristics.text: trichome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000282
characteristics.characteristic: tissue-type
characteristics.text: Samsun trichome library
characteristics.characteristic: title