characteristics.text: LIBEST_023644
characteristics.characteristic: External Id
characteristics.text: SAMN00166537
characteristics.characteristic: External Id
characteristics.text: Korea Polar Research Institute (KOPRI)
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:21.100Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Stratagene's cDNA Synthesis Kit (catalog number 200401), ZAP Express Vector Kit (catalog number 239211), and Gigapack III Gold Packaging Extract (catalog number 200201) were used to construct the cDNA library. After second-strand cDNA synthesis, adapter ligation, and size fractionation, the column eluent was then ligated into Stratagene's UniZAP XR vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. The ligated DNA was packaged using Stratagene's Gigapack III Gold Packaging Extract and the pBluescript SK phagemid and insert was circularized to phagemid by in vivo excision.
characteristics.characteristic: description
characteristics.text: Deschampsia antarctica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_159298
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Deschampsia antarctica Greenhouse Library
characteristics.characteristic: title
characteristics.text: pBluescript SK
characteristics.characteristic: vector