characteristics.text: SAMN00002154
characteristics.characteristic: External Id
characteristics.text: University of Washington, Department of Genome Sciences -Stamatoyannopoulos Lab
characteristics.characteristic: INSDC center name
characteristics.text: 2009-04-06T11:45:54.217Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:46:37.940Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS002204
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS002204
characteristics.characteristic: SRA accession
characteristics.text: MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 bar1Δ::KanMX (C. Boone, University of Toronto; S288c background derived from BY4741) Yeast strain and culture conditions S.cerevisiae R276 (MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 bar1Δ::KanMX) (C. Boone, University of Toronto; S288c background derived from BY4741), was cultured overnight in 50 ml rich medium (YPD) at 30*C, diluted into 500 ml fresh YPD to an OD660 of ~0.8, and treated with yeast alpha-factor (Sigma-Aldrich) at a final concentration of 50 ng / ml. The culture was incubated at 30 *C with shaking for 3 hours (final OD660 ~ 1). After this treatment, approximately 90% of the cells had formed mating projections when checked by light microscopy.
characteristics.characteristic: description
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Generic sample from Saccharomyces cerevisiae
characteristics.characteristic: title