characteristics.text: LIBEST_020079
characteristics.characteristic: External Id
characteristics.text: SAMN00150661
characteristics.characteristic: External Id
characteristics.text: Missouri State University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:37.233Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cabernet Sauvignon
characteristics.characteristic: cultivar
characteristics.text: Powdery mildew-infected and healthy leaves of Vitis vinifera 'Cabernet sauvignon' were harvested at 0, 4, 8, 12, 24, and 48 h after inoculation (mildew-infected) or 0, 4, 8, 12, 24, and 48 h after mock-inoculation (healthy control) inoculation as tissue source for SSH library. Total RNA was extracted individually for each time point of each infected and mock-inoculated sample. Equimolar amounts of RNA were pooled and reverse transcribed to cDNA using BD SMART PCR cDNA Synthesis Kit (Clontech, Paolo Alto, California). SSH library was constructed with PCR Select cDNA Subtraction Kit (Clontech), using infected tissue-derived cDNA as tester and mock-inoculated tissue-derived cDNA as driver. PCR-amplified inserts were sequenced with T7 or SP6 vector-specific primers.
characteristics.characteristic: description
characteristics.text: young leaf
characteristics.characteristic: dev-stage
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: leaf blade
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0020039
characteristics.characteristic: tissue-type
characteristics.text: All Infection Stages Subtractive Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-5zf(+)
characteristics.characteristic: vector