characteristics.text: SAMD00013302
characteristics.characteristic: External Id
characteristics.text: RNA, Institute for Advanced Biosciences, Keio University
characteristics.characteristic: INSDC center name
characteristics.text: 2011-08-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-11-12T03:29:32Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS000323
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS000323
characteristics.characteristic: SRA accession
characteristics.text: Escherichia coli K-12
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83333
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12 BW25113 cells were grown to late exponential phase (O.D.600 = 0.76) in modified M63 medium. Then, low molecular weight RNAs (<200 nt) were isolated from the cultured cells using mirVana miRNA Isolation Kit (Ambion). cDNA library was built from the RNA sample ligated with both 5' and 3' end specific RNA adapters for determining direction of each transcript.
characteristics.characteristic: sample comment
characteristics.text: DRS000323
characteristics.characteristic: sample name
characteristics.text: BW25113
characteristics.characteristic: strain
characteristics.text: low molecular weight RNA
characteristics.characteristic: title