characteristics.text: SAMN00161639
characteristics.characteristic: External Id
characteristics.text: LIBEST_007106
characteristics.characteristic: External Id
characteristics.text: Instituto de Investigaciones Biotecnologicas Universidad Nacional de San Martin
characteristics.characteristic: INSDC center name
characteristics.text: 2000-11-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:26.423Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized by reverse transcription of total RNA with oligo dT primer. cDNA was digested with RsaI and ligated with the adaptors 1 (5'-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT-3') and 2 (5'-GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT-3'). Two cDNA populations were then hybridizated and after that two PCR reactions were performed. The first one with the PCR primer 1 (5'-CTAATACGACTCACTATAGGGC-3') and the second reaction with Nested PCR primer 1 (5'-TCGAGCGGCCGCCCGGGCAGGT-3') and Nested PCR primer 2 (5'-AGCGTGGTCGCGGCCGAGGT-3'). These PCR products were cloned in pGEM-Teasy vector (Promega).
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: RsaI
characteristics.characteristic: re_1
characteristics.text: RsaI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sprague Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: hippocampus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002421
characteristics.characteristic: tissue-type
characteristics.text: PCR-SelectTM cDNA Subtraction Kit, Clontech. (cat# K1804-1)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy, Promega.
characteristics.characteristic: vector