characteristics.text: LIBEST_005649
characteristics.characteristic: External Id
characteristics.text: SAMN00160187
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-Food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:38.860Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Harosoy 63
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from polyA+ enriched mRNA from green seed coats in mid to late developemental stage, average fresh weight 250 mg per seed. Traces of pod and embryo tissue also present. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adopters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR.
characteristics.characteristic: description
characteristics.text: E. coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Seed coats
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009088
characteristics.characteristic: tissue-type
characteristics.text: GmaxSC
characteristics.characteristic: title
characteristics.text: pBK-CMV
characteristics.characteristic: vector