characteristics.text: LIBEST_022399
characteristics.characteristic: External Id
characteristics.text: SAMN00166460
characteristics.characteristic: External Id
characteristics.text: Beijing Institute of Genomics, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2009-03-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:43.343Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from tarantula leg muscle samples and checked for quality. The mRNA was isolated and converted to cDNA. cDNAs larger than 500bp were unidirectionally cloned into the pBluescript II XR Predigested Vector (Stratagene). The titre of the library was 4.0x105 pfu/ml. Clones were picked randomly and inserts end-sequenced using the ABI3730 sequencer.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: leg
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000978
characteristics.characteristic: organ
characteristics.text: Aphonopelma sp.
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29932
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: both male and female
characteristics.characteristic: sex
characteristics.text: skeletal muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001134
characteristics.characteristic: tissue-type
characteristics.text: Tarantula skeletal muscle library
characteristics.characteristic: title
characteristics.text: pBluescriptII
characteristics.characteristic: vector