characteristics.text: LIBEST_007315
characteristics.characteristic: External Id
characteristics.text: SAMN00161845
characteristics.characteristic: External Id
characteristics.text: National Eye Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:32.317Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies, essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: EMDH10B
characteristics.characteristic: lab-host
characteristics.text: Eye
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000970
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Lens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000965
characteristics.characteristic: tissue-type
characteristics.text: Human Lens cDNA (Un-normalized, unamplified): BY
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSPORT6
characteristics.characteristic: vector