characteristics.text: LIBEST_020328
characteristics.characteristic: External Id
characteristics.text: SAMN00150890
characteristics.characteristic: External Id
characteristics.text: The Hebrew University of Jerusalem
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:45.977Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Directional cDNA libraries were constructed using the Creator SMART cDNA Library Construction Kit (Clontech).Instars were collected from leaves of cotton plants caged with whiteflies (B.tabaci, B biotype) and held in insect-proof cages. RNA was isolated using TRIZOL and Phase Lock Gel-Heavy tubes. The first strand cDNA was synthesized using the total RNA and the CDS III/3' PCR Primer which contains a Sfi IB site. The cDNA was amplified by PCR: the first-strand cDNA was used together with the 5' PCR primer, which contains a Sfi IA, site and the CDS III/3' PCR primer. Following phenol treatment, the DNA was digested with SfiI and size-fractionated using CHROMA SPIN-400 Column. The high molecular weight cDNA fractions (with sticky ends) were pooled together and ligated with dephosphorylated pDNR-LIB vector treated Sfi IA and Sfi IB. The recombinant plasmids were electroporated into DH5_ and 10G competent cells, and plated on LB agar plate containing chloramphenicol.
characteristics.characteristic: description
characteristics.text: Cotton
characteristics.characteristic: lab-host
characteristics.text: Bemisia tabaci
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7038
characteristics.characteristic: organism
characteristics.text: Sfi IA
characteristics.characteristic: re_1
characteristics.text: Sfi IB
characteristics.characteristic: re_2
characteristics.text: B biotype
characteristics.characteristic: strain
characteristics.text: Whitefly Bemisia tabaci instar (crawler to pupae) cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector