characteristics.text: SAMN00150641
characteristics.characteristic: External Id
characteristics.text: LIBEST_020052
characteristics.characteristic: External Id
characteristics.text: Ocean University of China
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:35.490Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cells were collected at the logarithmic growth phase. Total RNA was extracted by the guanidine thiocyanate-phenol-chloroform extraction method (Chomczynski and Sacchi 1987). Message RNA was further purified according to the manufacturer's instructions of the Oligotex mRNA Extraction Kits (QIAGEN). Total RNA and mRNA were run on agarose gel to investigate the quality. cDNA synthesis was performed using the pBluescript II SK(+) XR cDNA Library Construction Kit (Stratagene Cloning Systems, California, USA). The library was created in Uni-Zap XR vector using oligo (dT)18 primers and directionally inserted into EcoR I-Xho I sites of pBluescript.
characteristics.characteristic: description
characteristics.text: Cells at the logarithmic growth phase
characteristics.characteristic: dev-stage
characteristics.text: Nannochloropsis oculata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_43925
characteristics.characteristic: organism
characteristics.text: unicellular microalgal cDNA Library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescriptII
characteristics.characteristic: vector