characteristics.text: LIBEST_006255
characteristics.characteristic: External Id
characteristics.text: SAMN00160791
characteristics.characteristic: External Id
characteristics.text: National Institute on Aging/National Institutes of Health
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:59.667Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNAs were extracted from 3000 Dopamine cells (cell collected by Dr.Tanya Barrett). The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.91ug of total RNA. The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Yulan Piao and Minoru.S. H. Ko.
characteristics.characteristic: description
characteristics.text: 13.5dpc
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Male/Female
characteristics.characteristic: sex
characteristics.text: TH-beta-gal transgenic mouse
characteristics.characteristic: strain
characteristics.text: NIA Mouse E13.5 VMB Dopamine cell cDNA Library
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pSPORT1 (Gibco/BRL Life Technology)
characteristics.characteristic: vector