characteristics.text: LIBEST_009641
characteristics.characteristic: External Id
characteristics.text: SAMN00164152
characteristics.characteristic: External Id
characteristics.text: Ocean University of Qingdao
characteristics.characteristic: INSDC center name
characteristics.text: 2002-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-06-28T21:04:23.963Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mature leafy gametophytes were rinsed extensively with boiled seawater, and cut into small pieces. Single tissue piece was cultivated in liquid Provasoli medium until filamentous sporophyte colonies come out. The most vigorous colony was cultivated and maintained as strains, such as Y9604, and the majority of them are pure genetically. Total RNA was isolated using UNIQ-10 Column Centrifugation Trizol Total RNA Isolation Kit (Sangon Inc., Shanghai, China, Fax: 021-64516369, Tel: 021-64519388). The cDNA was synthesized, amplified and cloned after size selection using cDNA Synthesis and cDNA PCR Library Kits, pMD18-T vector, PyrobestTM DNA polymerase and TaqTM DNA polymerase in combination (TaKaRa Biotechnology Co., Ltd., Dalian, China, Fax: 0411-7619946, Tel: 0411-7611410).
characteristics.characteristic: description
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Neopyropia yezoensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2788
characteristics.characteristic: organism
characteristics.text: RcoR V with a T overhang
characteristics.characteristic: re_1
characteristics.text: Y9604
characteristics.characteristic: strain
characteristics.text: Filamentous sporophyte
characteristics.characteristic: tissue-type
characteristics.text: Porphyra yezoensis filamentous sporophyte cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMD18-T
characteristics.characteristic: vector