characteristics.text: SAMN00154990
characteristics.characteristic: External Id
characteristics.text: LIBEST_000378
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:06.327Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:20:17.020Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified from 5 x 10E5 filariform larvae which had been isolated from experimentally infected dogs. cDNA was constructed and, using adaptors, was cloned unidirectionally into the vector from the EcoRI site to the XhoI site. The library has an unamplified titer of 3 x 10E5 pfu/ml and an amplified, undiluted titer of 1.5 x 10E10 pfu/ml. The average insert size of the unamplifed library is 850 bp (range, 200-1500).
characteristics.characteristic: description
characteristics.text: XL-1 Blue MRF' (Stratagene)
characteristics.characteristic: lab-host
characteristics.text: Strongyloides stercoralis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6248
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Filariform larvae obtained from dogs
characteristics.characteristic: strain
characteristics.text: TBN95TM-SsFd
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR (Stratagene)
characteristics.characteristic: vector