characteristics.text: SAMN00158634
characteristics.characteristic: External Id
characteristics.text: LIBEST_004082
characteristics.characteristic: External Id
characteristics.text: Biomedical Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:50.117Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hemocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000387
characteristics.characteristic: cell type
characteristics.text: B.glabrata BS-90 snails were exposed to miracidia (25/snail) from Schistosoma mansoni for 5 hr. Total RNA was isolated from the hemocytes and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector by Stratagene.
characteristics.characteristic: description
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: BS-90
characteristics.characteristic: strain
characteristics.text: Biomphalaria glabrata (BS-90)-exposed Lambda Zap Library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector