characteristics.text: LIBEST_000614
characteristics.characteristic: External Id
characteristics.text: SAMN00155216
characteristics.characteristic: External Id
characteristics.text: San Francisco General Hospital-University of California, San Francisco
characteristics.characteristic: INSDC center name
characteristics.text: 1997-02-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:15.703Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from purified Cryptosporidium parvum sporozoites using TRIZOL reagent (GIBCO-BRL), and contaminating DNA was removed by treatment with RNase-free Dnase I. Poly A+ RNA was prepared using the Promega Poly A Tract kit and directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I-Not I linkers, and digestion with Xho I. The cDNA was cloned into the EcoR I and Xho I sites of pBlueScript II (SK minus) plasmid vector (Stratagene), and transformed into XL2 Blue ultracompetent bacteria. The primary library was 97% recombinant and contained approximately 1 X 10(5) independent clones. PCR analysis of 20 random clones indicated that the average insert size was ca. 1.0 kb.
characteristics.characteristic: description
characteristics.text: sporozoite
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL2 Blue
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: IOWA
characteristics.characteristic: strain
characteristics.text: pSKIIminusCpIOWAsporoLib2
characteristics.characteristic: title
characteristics.text: pBlueScript II (SK minus)
characteristics.characteristic: vector