characteristics.text: SAMN00152933
characteristics.characteristic: External Id
characteristics.text: LIBEST_021190
characteristics.characteristic: External Id
characteristics.text: University of Nevada
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:11.870Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: MARC-145
characteristics.characteristic: cell-line
characteristics.text: Messenger RNA purified from PRRSV-infected MARC-145 cells at 72 hours post infection was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MR F' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.
characteristics.characteristic: description
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: MARC-145 cell line - PRRSV-exposed
characteristics.characteristic: title
characteristics.text: Lambda ZAPII
characteristics.characteristic: vector