characteristics.text: SAMN00153680
characteristics.characteristic: External Id
characteristics.text: LIBEST_022090
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-11-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:35.990Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 0.5-1kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: Transition to flowering
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Artemisia annua
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_35608
characteristics.characteristic: organism
characteristics.text: SfiI A
characteristics.characteristic: re_1
characteristics.text: SfiI B
characteristics.characteristic: re_2
characteristics.text: glandular trichomes with 10-20% palisade cells and hair trichomes
characteristics.characteristic: tissue-type
characteristics.text: CATC Artemisia annua, Tanzanian, from Sandeman Seeds, 0.5-1kb
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector