characteristics.text: SAMN00153917
characteristics.characteristic: External Id
characteristics.text: LIBEST_012104
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 2003-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:48.887Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ultra
characteristics.characteristic: cultivar
characteristics.text: Proteoid roots of white lupin grown under P deficient conditions were harvested 12 and 14 DAE (days after emergence), and poly(A)+ RNA was isolated. The poly(A)+ RNA obtained from plants 12 and 14 DAE was combined in a 1:1 ratio and 7 ug total RNA was used for reconstruction of a proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene.
characteristics.characteristic: description
characteristics.text: 12 and 14 DAE
characteristics.characteristic: dev-stage
characteristics.text: -P proteoid root (cluster root)
characteristics.characteristic: organ
characteristics.text: Lupinus albus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3870
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: -P proteoid roots 12 and 14 DAE
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBLUESCRIPT SK(+/-)
characteristics.characteristic: vector