characteristics.text: SAMN00002024
characteristics.characteristic: External Id
characteristics.text: CDC
characteristics.characteristic: INSDC center name
characteristics.text: 2011-06-28T11:51:07.973Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:44:39.120Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS002205
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS002205
characteristics.characteristic: SRA accession
characteristics.text: Equine neurological tissue (cortex, cerebellum, midbrain, hindbrain, thalamus, cervical and lumber spinal cord) and spleen were obtained by necropsy from three groups of two horses each (total of 6 individuals) and consisted of 1) naive horses infected with 1 X 105 WNV, intrathecally 2) non naive horses vaccinated for protection against WNV (Prevenile, Intervet-Schering-Plough) and infected with 1 X 105 WNV , intrathecally and 3) horses that were not infected or vaccinated Tissues were snap frozen and stored at -80oC until RNA extraction. Total RNA was extracted using Trizol (Guanidium thiocyanate- Invitrogen, Carlsbad, CA) and a traditional phase separation technique. Concentration and purity of each RNA sample was assessed using spectrophotometric technology (ND-1000, Nanodrop Technologies, Wilmington, DE) and degradation assessed by microfluidics (Agilent 2100 Bio-analyzer, Santa Clara, CA). The RNA was converted to a normalized cDNA library (SMART PCR cDNA synthesis kit and Advantage 2 PCR kit, Clontech, Mountainview, CA; QIAquick PCR Purification Kit, Qiagen, Valencia, California; and cDNA Normalization Trimmer Kit, Evrogen, Moscow, Russia).
characteristics.characteristic: description
characteristics.text: Equus caballus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9796
characteristics.characteristic: organism
characteristics.text: Generic sample from Equus caballus
characteristics.characteristic: title