characteristics.text: LIBEST_022778
characteristics.characteristic: External Id
characteristics.text: SAMN00165326
characteristics.characteristic: External Id
characteristics.text: CSIRO Plant Industry
characteristics.characteristic: INSDC center name
characteristics.text: 2008-02-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:54.713Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from ~30 yrs old trees in radiata pine were collected in autumn, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.
characteristics.characteristic: description
characteristics.text: Mature latewood, at ~30 yrs old and in autumn
characteristics.characteristic: dev-stage
characteristics.text: CSIRO Forest Biosciences
characteristics.characteristic: lab-host
characteristics.text: Pinus radiata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3347
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Developing xylem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0005352
characteristics.characteristic: tissue-type
characteristics.text: Radiata pine mature latewood cDNA library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Uni-ZAP XR
characteristics.characteristic: vector