characteristics.text: LIBEST_006111
characteristics.characteristic: External Id
characteristics.text: SAMN00160647
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:54.923Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Harosoy
characteristics.characteristic: cultivar
characteristics.text: The Harosoy NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from whole seedlings of 1 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). The library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.
characteristics.characteristic: description
characteristics.text: 1 week old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: whole seedlings of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1052
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector