characteristics.text: LIBEST_006114
characteristics.characteristic: External Id
characteristics.text: SAMN00160650
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:54.993Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from immature leaves (unfurled trifoliate) of greenhouse grown plants that were 2 week old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana-Champaign (email l-vodkin@uiuc.edu)
characteristics.characteristic: description
characteristics.text: 2 week old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: immature leaves (unfurled trifoliate) of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1047
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector