characteristics.text: LIBEST_007038
characteristics.characteristic: External Id
characteristics.text: SAMN00161572
characteristics.characteristic: External Id
characteristics.text: University Health Network
characteristics.characteristic: INSDC center name
characteristics.text: 2001-03-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:24.673Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: myeloma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001378
characteristics.characteristic: cell type
characteristics.text: Myeloma cells from multiple myeloma patients' bone marrow were purified by magnetic cell sorting. mRNA were purified and an oligo d(T)18 primer containing XhoI restriction site was used to prime first strand synthesis using M-MLV reverse transcriptase. To protect the cDNAs from XhoI digestion in subsequent cloning step, the nucleotide analogue 5-methyl-dCTP was added to the nucleotide mixture and [a-32P]dATP was added to monitor the quantity and quality of first strand synthesis. After second-strand synthesis and blunting of cDNA termini, EcoRI adapters were ligated, followed by kinase treatment and digestion with XhoI. The cDNAs were then size-fractionated using Sephacryl S-500 column and then ligated into EcoRI and XhoI digested Lambda Zap Express vector. The ligation product was packaged using Gigapack II packaging extract. The library had primary titre of approx. 1x106. Clones from the primary library were randomly selected for single pass sequencing.
characteristics.characteristic: description
characteristics.text: multiple myeloma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001378
characteristics.characteristic: dev-stage
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Myeloma (MYE) cDNA library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Zap Express
characteristics.characteristic: vector