characteristics.text: LIBEST_000248
characteristics.characteristic: External Id
characteristics.text: SAMN00154862
characteristics.characteristic: External Id
characteristics.text: Shemyakin Institute of Bioorganic Chemistry
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:06.770Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed as described in [Obradovic, D., Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V., Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S., Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20, 919-930]. This protocol is based on nested primer strategy using Alu- specific primers (ALN1 and 517) that direct the hncDNA synthesis inward of Alu repeats.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: BamH1
characteristics.characteristic: re_2
characteristics.text: Inward Alu-primed hncDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-3Z
characteristics.characteristic: vector