characteristics.text: SAMN00153780
characteristics.characteristic: External Id
characteristics.text: LIBEST_010364
characteristics.characteristic: External Id
characteristics.text: USDA/ARS/CCRU
characteristics.characteristic: INSDC center name
characteristics.text: 2002-06-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:02.200Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Morex
characteristics.characteristic: cultivar
characteristics.text: Lemma and palea were collected from greenhouse grown Morex barley in the elongation stage of kernel development. Total RNA was isolated from the pooled lemma and palea. Poly(A)+ RNA was isolated using an oligo(dT) matrix. Suppression subtractive hybridization (SSH) was performed to create lemma/palea-enriched cDNA library using the PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA). The cDNA obtained from lemma/palea mRNA was used as a tester and cDNA from leaf mRNA served as a driver. After filling-in the ends, the entire population of lemma/palea-enriched cDNA was subjected to two rounds of PCR. The subtracted cDNA library was ligated to pCR2.1-TOPO TA cloning vector (Invitrogen, Carlsbad, CA) and introduced into TOP10F' competent cells.
characteristics.characteristic: description
characteristics.text: Elongation kernel stage
characteristics.characteristic: dev-stage
characteristics.text: Lemma and palea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009038
characteristics.characteristic: organ
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: RsaI
characteristics.characteristic: re_1
characteristics.text: RsaI
characteristics.characteristic: re_2
characteristics.text: Lemma/palea-enriched cDNA library from elongation stage of kernel
characteristics.characteristic: title
characteristics.text: pCR2.1-TOPO
characteristics.characteristic: vector