characteristics.text: SAMN00166056
characteristics.characteristic: External Id
characteristics.text: LIBEST_012297
characteristics.characteristic: External Id
characteristics.text: UMR INRA/Universite Henri Poincare 1136
characteristics.characteristic: INSDC center name
characteristics.text: 2003-02-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:53.743Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: clone 'INRA 717-1-B4'
characteristics.characteristic: cultivar
characteristics.text: cDNA library of three-week-old roots from Populus tremula L. x P.alba L. (clone 'INRA 717-1-B4') incubated in 5uM indole-3-acetic acid 24h before harvesting. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).
characteristics.characteristic: description
characteristics.text: three-week-old
characteristics.characteristic: dev-stage
characteristics.text: root
characteristics.characteristic: organ
characteristics.text: Populus tremula x Populus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80863
characteristics.characteristic: organism
characteristics.text: three-week-old roots incubated in 5uM indole-3-acetic acid 24h before harvesting
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector