characteristics.text: LIBEST_020694
characteristics.characteristic: External Id
characteristics.text: SAMN00152483
characteristics.characteristic: External Id
characteristics.text: Genome Institute of Singapore
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:57.347Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Priming method: GsuI-d(T) priming ; Priming sequence: 5' GAGCTAGTTCTGGAG(T)16VN ; Directionally cloned, 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' GGATCCGAC 3' ; 3' cloning site: BseRI ; Insert size range: 2.5kbp-700bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 94% ; Library complexity: 6.38 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented less abundantly expressed clones. The hybridization intensities for all clones span from 0 to 1 million counts and the low abundant selected clones in this library ranges from 0 to 45,000.
characteristics.characteristic: description
characteristics.text: Adult fish of 3-4 months old
characteristics.characteristic: dev-stage
characteristics.text: Escherichia Top10
characteristics.characteristic: lab-host
characteristics.text: Brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: BseRI
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: Males and females mixed
characteristics.characteristic: sex
characteristics.text: Tubingen (TU)
characteristics.characteristic: strain
characteristics.text: Brain (including tissues and cartilage around brain)
characteristics.characteristic: tissue-type
characteristics.text: FDR103
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGIS4a
characteristics.characteristic: vector