characteristics.text: LIBEST_004083
characteristics.characteristic: External Id
characteristics.text: SAMN00158635
characteristics.characteristic: External Id
characteristics.text: Biomedical Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:50.137Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hemocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000387
characteristics.characteristic: cell type
characteristics.text: Total RNA was isolated from the hemocytes of unexposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector by Stratgene.
characteristics.characteristic: description
characteristics.text: Laboratory host
characteristics.characteristic: lab-host
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: BS-90
characteristics.characteristic: strain
characteristics.text: Biomphalaria glabrata (BS-90)-unexposed Lambda Zap Library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector