characteristics.text: LIBEST_005584
characteristics.characteristic: External Id
characteristics.text: SAMN00160122
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Plant Science and Entomology Unit
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:37.013Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sumai3
characteristics.characteristic: cultivar
characteristics.text: Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
characteristics.characteristic: description
characteristics.text: Adult plant
characteristics.characteristic: dev-stage
characteristics.text: E. coli JM109
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: SacII
characteristics.characteristic: re_1
characteristics.text: SpeI
characteristics.characteristic: re_2
characteristics.text: Spike
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001278
characteristics.characteristic: tissue-type
characteristics.text: KSU wheat Fusarium graminearum infected spike cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector