characteristics.text: LIBEST_009717
characteristics.characteristic: External Id
characteristics.text: SAMN00164223
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-03T23:44:11.793Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:40:24.520Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cheyenne
characteristics.characteristic: cultivar
characteristics.text: Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech University. Normalization protocol used was that of Soares' with modification.
characteristics.characteristic: description
characteristics.text: 5 -30 days post anthesis seed
characteristics.characteristic: dev-stage
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Endosperm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009089
characteristics.characteristic: tissue-type
characteristics.text: Wheat endosperm normalized cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP II, excised phagemid
characteristics.characteristic: vector