characteristics.text: SAMN00160094
characteristics.characteristic: External Id
characteristics.text: LIBEST_005556
characteristics.characteristic: External Id
characteristics.text: National University of Ireland Galway (NUIG)
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:35.953Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: An Atlantic salmon ovary cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organ
characteristics.text: Salmo salar
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8030
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: tissue-type
characteristics.text: Atlantic salmon Lambda Zap Express ovary cDNA library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP Express
characteristics.characteristic: vector