characteristics.text: LIBEST_004050
characteristics.characteristic: External Id
characteristics.text: SAMN00158603
characteristics.characteristic: External Id
characteristics.text: Michigan State University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:49.407Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: USH20
characteristics.characteristic: cultivar
characteristics.text: cDNAs were derived from reverse transcription of pooled mRNA samples from stressed seedlings (germinated for 4 days in 150mM NaCl, 200mM Mannitol, submerged in distilled water and 0.3% Hydrogen peroxide). The cDNA library was generated by directional ligation of the cDNAs in the EcoRI and XhoI sites of Lambda Uni-Zap XR vector (Stratagene). The library was excised as a pBluescript plasmid from Lambda Uni-Zap XR by ExAssist helper phage and plated on SOLR host cells.
characteristics.characteristic: description
characteristics.text: 4-day germination under stress (salt/NaCl, dehydration/Mannitol and anaerobic stress)
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: seeds
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000991
characteristics.characteristic: organ
characteristics.text: Beta vulgaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_161934
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: whole seedlings
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Sugar Beet germination cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II KS (+) excised from Lambda Uni-Zap XR
characteristics.characteristic: vector