characteristics.text: LIBEST_009841
characteristics.characteristic: External Id
characteristics.text: SAMN00164344
characteristics.characteristic: External Id
characteristics.text: Clemson University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.260Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Morex
characteristics.characteristic: cultivar
characteristics.text: Plants were grown at the University of Minnesota in the GJ Muehlbauer lab; spikes were harvested and snap frozen at 0, 1, 2, 3, 4, 5, 6, and 8 days after Fusarium graminearum inoculation (Heinen). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all eight RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Fenton, Malatrasi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)
characteristics.characteristic: description
characteristics.text: TJC121
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Spike
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001278
characteristics.characteristic: tissue-type
characteristics.text: Hordeum vulgare spike EST library HVcDNA0012 (Fusarium infected)
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector