characteristics.text: LIBEST_021980
characteristics.characteristic: External Id
characteristics.text: SAMN00153579
characteristics.characteristic: External Id
characteristics.text: National Institute of Biomedical Innovation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-10-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:32.387Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.
characteristics.characteristic: description
characteristics.text: 11 years old
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004340
characteristics.characteristic: dev-stage
characteristics.text: TOP10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007796
characteristics.characteristic: lab-host
characteristics.text: Pan troglodytes verus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_37012
characteristics.characteristic: organism
characteristics.text: DraIII (CACTGTGTG)
characteristics.characteristic: re_1
characteristics.text: DraIII (CACCATGTG)
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: brain frontal lobe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001870
characteristics.characteristic: tissue-type
characteristics.text: chimpanzee brain cDNA library PflC
characteristics.characteristic: title
characteristics.text: pME18S-FL3 (Acc.No. AB009864)
characteristics.characteristic: vector