characteristics.text: LIBEST_019634
characteristics.characteristic: External Id
characteristics.text: SAMN00154317
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:23.660Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: LxS8
characteristics.characteristic: cultivar
characteristics.text: Library was prepared using the PCR-Select method. Desired mRNA was isolated from floral nectary tissues at Stage 6 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants. Control (driver) mRNA was harvested from floral nectary tissues at Stage 12 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants. Following second strand synthesis of desired Stage 6 cDNA, the cDNAs were sheared to approximately 300 nucleotides in length. Subtraction was Stage 6 sequences minus Stage 12 sequences.
characteristics.characteristic: description
characteristics.text: Floral stage 6
characteristics.characteristic: dev-stage
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: Floral nectary
characteristics.characteristic: organ
characteristics.text: Nicotiana langsdorffii x Nicotiana sanderae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_164110
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Ornamental tobacco (LxS8) Stage 6 Floral Nectary subtracted cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1TOPO
characteristics.characteristic: vector