characteristics.text: SAMN00165718
characteristics.characteristic: External Id
characteristics.text: LIBEST_023141
characteristics.characteristic: External Id
characteristics.text: Tamil Nadu Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:05.730Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Rice plants (Oryza sativa ssp. Indica cv. Co 43) were grown under green house conditions. Fifty day old plants were inoculated with insects (leaf folder and BPH) and pathogens (R. solani). One day before challenging, a set of plants were treated with PGPR (Pseudomonas fluorescens strain TDK-1). Forty eight hours after inoculation, observations were made on pest/disease development based on the symptoms. Leaf sheaths were collected in liquid nitrogen from the following treatments for RNA isolation. Treatment details: 1. Control 3. Leaf folder alone 5. BPH alone 7. R. solani alone 2. PGPR alone 4. Leaf folder + PGPR 6. BPH + PGPR 8. TDK-1 + R. solani. Total RNA was extracted from the leaf sheath tissues collected from the above treatments using the TRIzol reagent method (Invitrogen, San Diego, USA). DD-RT-PCR experiment was performed to identify the differentially expressed cDNAs during different treatmental conditions using RNAimage system (GeneHunter Corporation, USA). Differentially expressed cDNA fragments were excised from the gel, reamplified and cloned in vector (Fermentas, St. Leon- Rot, Germany). Cloned cDNAs were sequenced by Automated cycle sequencing and raw EST sequences were edited to remove adoptor and vector sequences using the Lucy software programme (Chou and Holmes, 2001). Sequence analysis was performed to identify the putative function of the differentially expressed genes by searching against sequences deposited in NCBI and TIGR databases.
characteristics.characteristic: description
characteristics.text: 50 days after sowing
characteristics.characteristic: dev-stage
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Shoot
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000992
characteristics.characteristic: organ
characteristics.text: Oryza sativa Indica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39946
characteristics.characteristic: organism
characteristics.text: Hind III
characteristics.characteristic: re_1
characteristics.text: Hind III
characteristics.characteristic: re_2
characteristics.text: Sheath
characteristics.characteristic: tissue-type
characteristics.text: Differential genes in Rice plants, Rhizoctonia solani challenged Rice cDNA
characteristics.characteristic: title
characteristics.text: Plasmid (T/ A cloning)
characteristics.characteristic: v_type
characteristics.text: pGEMT
characteristics.characteristic: vector