characteristics.text: LIBEST_021254
characteristics.characteristic: External Id
characteristics.text: SAMN00152990
characteristics.characteristic: External Id
characteristics.text: Lund University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:13.500Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The insects were kept at 23 C, 70 percent humidity and a 18:6 h Light:Dark cycle. Total RNA was extracted from ovipositors including pheromone glands from 10 females 0-1 day post emergence, 1.5-2 h into the scotophase. The cDNA library was constructed using the SMART cDNA Construction Kit (BD Biosciences) according to the manufacturers' protocol. Full length cDNAs were directionally ligated into (lambda) TriplEx2 arms after trimming by SfiI. The lambda library was converted into a plasmid library in a Cre+ b/w strain (E. coli BNN132). Plasmid clones were collected randomly and used as template for PCR amplification of inserts using the plasmid-specific primers P104 and P105, followed by DNA sequencing using the forward primer P104.
characteristics.characteristic: description
characteristics.text: Adult 0-1 day post emergence
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli BNN132
characteristics.characteristic: lab-host
characteristics.text: Agrotis segetum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_47767
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: As12, Biologische Bundesanstalt fur Land- und Fortwirtschaft, Darmstadt, Germany
characteristics.characteristic: strain
characteristics.text: Pheromone gland
characteristics.characteristic: tissue-type
characteristics.text: As12G - Agrotis segetum pheromone gland library
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector