characteristics.text: LIBEST_020344
characteristics.characteristic: External Id
characteristics.text: SAMN00150910
characteristics.characteristic: External Id
characteristics.text: School of life science, Sun Yat-sen university
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:46.393Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA of stressed by 3% salinity and unstressed samples were both extracted from their leaves. Double-stranded tester cDNA and driver cDNA were prepared from 0.4ug total RNA of each sample by using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's protocol. The forward substracted library was constructed using the PCR-select cDNA Subtraction Kit (Clontech). Amplified, differentially expressed cDNA fragments were cloned into the pGEM T easy vector (promega). A total of 625 white clones were picked and cultured for plasmid extraction.
characteristics.characteristic: description
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Aegiceras corniculatum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_59970
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Aegiceras corniculatum suppressive substractive hybridization library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM T easy vector(Promega)
characteristics.characteristic: vector