characteristics.text: LIBEST_022136
characteristics.characteristic: External Id
characteristics.text: SAMN00153717
characteristics.characteristic: External Id
characteristics.text: State University of Campinas
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.050Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: somatic
characteristics.characteristic: cell type
characteristics.text: Total RNA was extracted from the monokaryotic biotrophic mycelium of M. perniciosa grown for 30 days on minimal media containing glycerol and a filtered extract of cacao meristems as carbon sources, and 0.5% yeast extract as nitrogen source. Library was prepared from total RNA using the BD Creator TM SMART TM cDNA Library Construction kit (BD Biosciences-ClontechTM, USA, Palo Alto, CA), following the manufacturer's protocol, and cloned into the pDNR-LIB plasmid vector provided in the kit.
characteristics.characteristic: description
characteristics.text: biotrophic
characteristics.characteristic: dev-stage
characteristics.text: Theobroma cacao L.
characteristics.characteristic: lab-host
characteristics.text: Moniliophthora perniciosa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_153609
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: CP02
characteristics.characteristic: strain
characteristics.text: monokaryotic mycelium
characteristics.characteristic: tissue-type
characteristics.text: E-Bio-CM
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB (BD Biosciences-ClontechTM, USA, Palo Alto, CA)
characteristics.characteristic: vector