characteristics.text: LIBEST_009655
characteristics.characteristic: External Id
characteristics.text: SAMN00164164
characteristics.characteristic: External Id
characteristics.text: Baylor College of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.423Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A17
characteristics.characteristic: cultivar
characteristics.text: Immature pods, ranging in age from 15 to 30 days after pollination, were collected from greenhouse-grown plants. At harvest, seeds were removed from pods and isolated pod walls were collected and immediately frozen in liquid nitrogen. Pod walls were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells.
characteristics.characteristic: description
characteristics.text: Immature pods, ranging in age from 15 to 30 days after pollination
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: immature pod walls
characteristics.characteristic: tissue-type
characteristics.text: GPOD
characteristics.characteristic: title
characteristics.text: pBluescript SK-
characteristics.characteristic: vector