characteristics.text: LIBEST_003745
characteristics.characteristic: External Id
characteristics.text: SAMN00158301
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-02-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:41.147Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This cDNA library was constructed from mRNA isolated from immature cotyledons of greenhouse grown plants (individual seed fresh weight of 100-300mg). The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restriction site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna. Note that Gm-r1030 is a re-rack of Gm-c1007.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Gm-r1030
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector