characteristics.text: LIBEST_022554
characteristics.characteristic: External Id
characteristics.text: SAMN00164789
characteristics.characteristic: External Id
characteristics.text: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:47.883Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Barke
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0004518
characteristics.characteristic: cultivar
characteristics.text: mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700bp
characteristics.characteristic: description
characteristics.text: developing caryopsis (3.-15.DAP)
characteristics.characteristic: dev-stage
characteristics.text: XLOLR
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: developing caryopsis
characteristics.characteristic: tissue-type
characteristics.text: HY
characteristics.characteristic: title
characteristics.text: pBK-CMV
characteristics.characteristic: vector