characteristics.text: LIBEST_015545
characteristics.characteristic: External Id
characteristics.text: SAMN00154078
characteristics.characteristic: External Id
characteristics.text: The University of Georgia, Department of Plant Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2004-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:26.840Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: BTx623
characteristics.characteristic: cultivar
characteristics.text: The library was prepared from polyA+ RNA from oxidatively stressed, hydroponically grown sorghum seedlings. At 8 days of age, growth medium was supplemented with hydrogen peroxide to 0.003% and leaves were misted with 10 uM methyl viologen. Leaves and roots were harvested at 3, 12 and 27 hr after treatment and all tissue pooled. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.
characteristics.characteristic: description
characteristics.text: DH10B-T1 phage-resistant E. coli
characteristics.characteristic: lab-host
characteristics.text: Leaf and Root
characteristics.characteristic: organ
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Oxidatively-stressed leaves and roots
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pME18S-FL3
characteristics.characteristic: vector