characteristics.text: LIBEST_005411
characteristics.characteristic: External Id
characteristics.text: SAMN00159949
characteristics.characteristic: External Id
characteristics.text: Texas Children's Cancer Center and Human Genome Sequencing Center at Baylor College of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:29.887Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: pre-B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000817
characteristics.characteristic: cell type
characteristics.text: First strand cDNA was primed with an anchored XhoI-oligo(dT) primer [5'GGAGGACTCGAGCGGCCGCAGGAGGAG(T)VN 3'; V=A,C,G; N=A,C,G,T] and then dG tailed. Second strand was primed with a BamH1-dC primer [5'AGAGAGCTCGGATCCGCGGCCGCAATAATAATAAT(C) 3']. Double-stranded cDNA was then digested with BamH1 and XhoI and directionally cloned into the BamH1 and Sa1I sites of lambda pSB vector. Library went through one round of normalization. Library was constructed by Wei Yu at RIKEN of Japan (Carninci P, Westover A, Nishiyama Y, Ohsumi T, Itoh M, Nagaoka S, SasakiN, Okazaki Y, Muramatsu M, Schneider C, Hayashizaki Y, High efficiency selection of full-length cDNA by improved biotinylated cap trapper., DNA Res 4: 1, 61-6, Feb 28, 1997)
characteristics.characteristic: description
characteristics.text: pediatric 2 years
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: leukopheresis
characteristics.characteristic: tissue-type
characteristics.text: Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA
characteristics.characteristic: title
characteristics.text: lambda pSB
characteristics.characteristic: vector