characteristics.text: SAMN00152988
characteristics.characteristic: External Id
characteristics.text: LIBEST_021250
characteristics.characteristic: External Id
characteristics.text: University of Texas at Austin
characteristics.characteristic: INSDC center name
characteristics.text: 2017-09-18T00:50:03.493Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:13.400Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Vegetative
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025223
characteristics.characteristic: cell type
characteristics.text: mRNA was isolated from late log-phase axenic cell cultures collected at various times during the light and dark phases of the photoperiod, which was later pooled. Directional cloning of cDNA positioned the 5' end of the insert at the EcoRI site; the poly-A(+) tail is located at the XhoI site of the plasmid. Part or all of the EcoRI linker sequence (GGCACGAGG) may be present at the 5' end of the sequence.
characteristics.characteristic: description
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Prymnesium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_97485
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end)
characteristics.characteristic: re_2
characteristics.text: Asexual
characteristics.characteristic: sex
characteristics.text: UTEX 2797 (Texas isolate)
characteristics.characteristic: strain
characteristics.text: Haptophyte (alga)
characteristics.characteristic: tissue-type
characteristics.text: PPcD
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II XR (Stratagene)
characteristics.characteristic: vector