characteristics.text: SAMN00025372
characteristics.characteristic: External Id
characteristics.text: Universidad Miguel Hernandez - Evolutionary Genomics Group
characteristics.characteristic: INSDC center name
characteristics.text: 2011-09-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:58:40.080Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS085785
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS085785
characteristics.characteristic: SRA accession
characteristics.text: Spain: Alicante; Bras del Port salterns
characteristics.characteristic: country
characteristics.text: The sample was collected from Bras del Port salterns in Alicante, Spain . The concentrator pond (SS19) was sampled on July 21, 2008. The salinity was measured using a hand refractometer (salinity 19%). The sample was sequentially filtered through a 5 micron and 0.22 micron-pore size polycarbonate filters using a peristaltic pump. Filters were stored at -80 deg C until DNA extraction. DNA was extracted as described before (Martin-Cuadrado et al, 2007), briefly, filters were thawed on ice and then treated with 1 mg/ml lysozyme and 0.2 mg/ml proteinase K (final concentrations). Nucleic acids were extracted with phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol and DNA integrity was checked by agarose gel electrophoresis. 5 micrograms of DNA sent separately for sequencing (Roche 454 GS-FLX system, Titanium chemistry, by GATC, Konstanz, Germany) (one full plate 454).
characteristics.characteristic: description
characteristics.text: saltern metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_496920
characteristics.characteristic: organism
characteristics.text: Solar Saltern Metagenome Sample SS19
characteristics.characteristic: title