characteristics.text: SAMN00164597
characteristics.characteristic: External Id
characteristics.text: LIBEST_022361
characteristics.characteristic: External Id
characteristics.text: China Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:42.347Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Inbred line S1
characteristics.characteristic: cultivar
characteristics.text: PCR-select cDNA subtraction was performed using a Clontech kit according to established protocols. The cDNA from treated seedlings with 4h low light was used as a tester and the cDNA from untreated seedlings used as a driver. The cDNAs were digested with RsaI and then ligated to different adapters. Two rounds of hybridization and PCR amplification were processed to normalize and enrich the differentially expressed cDNAs. Products of the secondary PCR from the forward subtraction were directly inserted into pGEMB. T-easy Vector (Promega) and transformed into E. coli DH5N1 cells. All the recombinant clones were picked up to establish the subtracted cDNA library.
characteristics.characteristic: description
characteristics.text: After the emergence of the three primary leaves
characteristics.characteristic: dev-stage
characteristics.text: Cucumis sativus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3659
characteristics.characteristic: organism
characteristics.text: Leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Low-light stress specific subtracted cDNA library of cucumber seedlings
characteristics.characteristic: title
characteristics.text: pGEMB. T-easy Vector (Promega)
characteristics.characteristic: vector