characteristics.text: LIBEST_009821
characteristics.characteristic: External Id
characteristics.text: SAMN00164325
characteristics.characteristic: External Id
characteristics.text: Harvard University, Howard Hughes Medical Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:47.540Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Starting library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.08 kb. Library was amplified once on solid support and plasmid DNA from library was prepared. The library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded library plasmid DNA was mixed with 5 micrograms PCR product representing library inserts and hybridized to an Ecot of 20. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Pancreas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001264
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not 1
characteristics.characteristic: re_1
characteristics.text: Sal 1
characteristics.characteristic: re_2
characteristics.text: Both
characteristics.characteristic: sex
characteristics.text: Islets of Langerhans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000006
characteristics.characteristic: tissue-type
characteristics.text: Melton Normalized Human Islet 4 N4-HIS 1
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pSPORTl
characteristics.characteristic: vector