characteristics.text: SAMN00152854
characteristics.characteristic: External Id
characteristics.text: LIBEST_021099
characteristics.characteristic: External Id
characteristics.text: Kyungpook National University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:09.423Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: dermal papilla
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000346
characteristics.characteristic: cell type
characteristics.text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from balding frontal hair. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.
characteristics.characteristic: description
characteristics.text: Primary culture
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002660
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: skin
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000014
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: frontal balding hair
characteristics.characteristic: tissue-type
characteristics.text: Human cultured dermal papilla cell cDNA library(K)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector