characteristics.text: SAMN00160029
characteristics.characteristic: External Id
characteristics.text: LIBEST_005491
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:33.937Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Chinese Spring
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007588
characteristics.characteristic: cultivar
characteristics.text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
characteristics.characteristic: description
characteristics.text: Five day old etiolated seedling
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Root
characteristics.characteristic: tissue-type
characteristics.text: Wheat etiolated seedling root normalized cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK
characteristics.characteristic: vector