characteristics.text: LIBEST_022921
characteristics.characteristic: External Id
characteristics.text: SAMN00165467
characteristics.characteristic: External Id
characteristics.text: USDA, Agricultural Research Service
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:59.190Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Canadian Harmony on Tennessee Natural rootstock
characteristics.characteristic: cultivar
characteristics.text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.
characteristics.characteristic: description
characteristics.text: current year's growth
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5alpha
characteristics.characteristic: lab-host
characteristics.text: branch
characteristics.characteristic: organ
characteristics.text: Prunus persica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3760
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: monoecious
characteristics.characteristic: sex
characteristics.text: bark (mostly cambium)
characteristics.characteristic: tissue-type
characteristics.text: Subtraction suppressive hybridization library E Reverse
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector