characteristics.text: LIBEST_006258
characteristics.characteristic: External Id
characteristics.text: SAMN00160794
characteristics.characteristic: External Id
characteristics.text: University of Illinois at Urbana-Champaign
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:59.727Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was prepared by Stratagene using oligo-T and random priming and bidirectional cloning with an adaptor (GGCACGAG) on each end (although not all clones have the expected adaptor sequence after the EcoRI site). Many of the clones have multiple insertions, and where those are known their chimeric nature is noted in the EST description. The mRNA was prepared from antennae of adult female honey bees. Clones were subcloned in vivo in mass into pBluescript maintained in SOLR cells for DNA sequencing.
characteristics.characteristic: description
characteristics.text: adults
characteristics.characteristic: dev-stage
characteristics.text: XL1 Blue MRF' and SOLR
characteristics.characteristic: lab-host
characteristics.text: antennae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000972
characteristics.characteristic: organ
characteristics.text: Apis mellifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7460
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Apis mellifera female antennae Lambda ZAP II library
characteristics.characteristic: title
characteristics.text: lambda virus
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP II
characteristics.characteristic: vector