characteristics.text: LIBEST_010930
characteristics.characteristic: External Id
characteristics.text: SAMN00153873
characteristics.characteristic: External Id
characteristics.text: Crop and Food Research
characteristics.characteristic: INSDC center name
characteristics.text: 2002-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:18.320Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Pukekohe Longkeeper
characteristics.characteristic: cultivar
characteristics.text: A. cepa seedlings were grown hydroponically (Randle et al., 1995) until bulbing. RNA was extracted from bulb meristem tissue and cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssist helper phage and propagated in SOLR cells. Clones were randomly chosen for sequencing.
characteristics.characteristic: description
characteristics.text: bulbing
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: bulb meristem
characteristics.characteristic: organ
characteristics.text: Allium cepa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4679
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: onion bulb meristem library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript pSK
characteristics.characteristic: vector