characteristics.text: SAMN00165607
characteristics.characteristic: External Id
characteristics.text: LIBEST_023042
characteristics.characteristic: External Id
characteristics.text: Genetic Engineering and Biotechnology Inst.
characteristics.characteristic: INSDC center name
characteristics.text: 2009-05-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:03.157Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Atay 85
characteristics.characteristic: cultivar
characteristics.text: Four week -old abscisic acid (ABA) teated and untreated wheat plants were harvested. Total RNA was isolated from 50 uM ABA treated and untreated plants. After RT-PCR, 3 different one-base anchored oligo dT primers were used in combination with series of arbitrary 13 mers for mRNA DD reactions. Amplified products were loaded on polyacrylamide gel and differentially expressed transcripts were isolated and amplified by PCR. PCR products were cloned to PCR-TRAP vector, digested with HindIII for the confirmation of the positive clones and then sequenced.
characteristics.characteristic: description
characteristics.text: 30 day-old
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH5
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: HindIII
characteristics.characteristic: re_1
characteristics.text: HindIII
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: ABA treated Bread wheat (T.aestivum L.)leaves
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: PCR-TRAP
characteristics.characteristic: vector