characteristics.text: SAMN00158585
characteristics.characteristic: External Id
characteristics.text: LIBEST_004032
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:09.900Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:25:19.027Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: fibroblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000057
characteristics.characteristic: cell type
characteristics.text: Anchor enzyme: NlaIII~ Large T antigen transformed human fibroblasts clones, harvested from skin tissue, that have not reached the cell crisis state. These cells were grown in MEM (Minimum Essential Media) with 10% FBS, 5% CO2 and harvested at passages 4-8.~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487.~ Tissue supplier: John Murnane~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Sph1
characteristics.characteristic: re_1
characteristics.text: Sph1
characteristics.characteristic: re_2
characteristics.text: skin
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000014
characteristics.characteristic: tissue-type
characteristics.text: SAGE_Duke_precrisis_fibroblasts
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pZErO-1
characteristics.characteristic: vector