characteristics.text: SAMN00004949
characteristics.characteristic: External Id
characteristics.text: UMass Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 2010-06-28T14:43:51.037Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:51:50.750Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS007048
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS007048
characteristics.characteristic: SRA accession
characteristics.text: Heads were dissected by vigorous shaking in liquid nitrogen in a metal sieve, as described in Seitz et al. (Curr. Biol. 18:147-151 (2008)). Total RNA was extracted using the mirVana kit (Ambion), then short (18-29 nt) RNAs were gel-purified. 2S rRNA was depleted as described in Seitz et al. (Curr. Biol. 18:147-151 (2008)). When applicable: small RNA preparations were oxidized with periodate in the presence of boric acid as described in Horwich et al. (2007) Curr. Biol. 17(14):1265-1272, except the final (alkaline) step was omitted. CyO-balanced hen1^f00810 mutant strain (described in Horwich et al., 2007, Curr. Biol. 17: 1265)
characteristics.characteristic: description
characteristics.text: hen1 heterozygous
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: heads
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000033
characteristics.characteristic: tissue
characteristics.text: Drosophila melanogaster heads
characteristics.characteristic: title