characteristics.text: LIBEST_000106
characteristics.characteristic: External Id
characteristics.text: SAMN00154721
characteristics.characteristic: External Id
characteristics.text: Yale University
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:03.203Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A yeast genomic DNA library was prepared in the vector pHSS6, and subjected to transposon mutagenesis with mTn3. This mini-transposon carries lacZ sequences that lack an initiation codon; expression of lacZ is only provided by in frame fusion to yeast coding sequence. The yeast genomic DNA carrying the transposon was excised from pHSS6 and transplaced back onto the yeast chromosome. Yeast colonies expressing lacZ were screened for in a color assay. A plasmid containing the genomic DNA/lacZ fusion junction was recovered from each individual yeast colony that expressed lacZ activity. These recovered plasmids comprise 'The blue guys library'. The fusion junction was then sequenced to identify the expressed ORF upstream of the fusion.
characteristics.characteristic: description
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: The blue guys library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pRECmTn
characteristics.characteristic: vector