characteristics.text: LIBEST_021972
characteristics.characteristic: External Id
characteristics.text: SAMN00153573
characteristics.characteristic: External Id
characteristics.text: Northwest A & F University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-10-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:32.183Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: young leaves
characteristics.characteristic: cell type
characteristics.text: Total RNA was extracted by Biozol, reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction method Clontech company cDNA from the inoculated leaves used as the tester, cDNA from the control (no-inoculation) used as the driver, after twice hybridization the PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by M13+ primer.
characteristics.characteristic: description
characteristics.text: leaf materials collected at three-leaf stage, after 3 days inoculation with the dominant race of powdery mildew pathogen in Guanzhong of Shaanxi
characteristics.characteristic: dev-stage
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: DH5a
characteristics.characteristic: strain
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: SSH-cDNA Library of powdery mildew infection in leaves of powdery mildew resistant variety N9820 compared with no infection
characteristics.characteristic: title