characteristics.text: SAMN00156520
characteristics.characteristic: External Id
characteristics.text: LIBEST_001957
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-07-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:55.610Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from leaves of 2-3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a 3' anchored poly (dT) primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and HindIII. The cDNA fragments were directionally cloned into the EcoRI-HindIII restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: HindIII
characteristics.characteristic: re_2
characteristics.text: Leaves, 2-3 week old seedlings, greenhouse grown
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1014
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT7T3Pac (pT7T3, Pharmacia)
characteristics.characteristic: vector