characteristics.text: LIBEST_006787
characteristics.characteristic: External Id
characteristics.text: SAMN00161322
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:16.490Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63422.
characteristics.characteristic: description
characteristics.text: 60 day
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000033
characteristics.characteristic: dev-stage
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: testis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000473
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: CD-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005180
characteristics.characteristic: strain
characteristics.text: spermatocytes, pooled from multiple mice
characteristics.characteristic: tissue-type
characteristics.text: McCarrey Eddy spermatocytes
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK+ (Stratagene)
characteristics.characteristic: vector