characteristics.text: LIBEST_021631
characteristics.characteristic: External Id
characteristics.text: SAMN00153338
characteristics.characteristic: External Id
characteristics.text: Department of Chemistry and Biomolecular Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:23.217Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A non-directional cDNA library was constructed in the plasmid vector pGEM-T (Promega) by T-ended cloning, according to the manufacturer's protocol. Total RNA was extracted from the adults of Dictyocaulus viviparus (strain HannoverDv2000) using the TriPure isolation reagent (Roche Molecular Biochemicals). Adult worms of D. viviparus were produced in helminth-free male Holstein-Friesian calves (five months of age). The worms were washed extensively (five times) in large volumes (100 ml) of fresh diethyl-pyrocarbonate (DEPC)-treated saline (22 oC), transferred to sterile, RNase-free screw-top cryovials (4 ml; Roth, Karlsruhe, Germany) and frozen as 200 ul pellets in a minimal amount of saline at -75 oC or -196 oC until RNA isolation. Integrity and yields of RNA were verified and estimated, respectively, using the Bioanalyzer 2100 (Agilent). Each RNA sample (~10 g) was treated with 2 U of DNase I (Promega), incubated at 37 oC for 30 min prior to heat denaturation of the enzyme (75 oC for 5 min) and then stored at -70oC until use. The cDNA was produced from 10 ug of total RNA from D. viviparus, using an oligo (dT) primer and Superscript II reverse transcriptase (Invitrogen) and then purified over DyeEx columns (Qiagen).
characteristics.characteristic: description
characteristics.text: Dictyocaulus viviparus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29172
characteristics.characteristic: organism
characteristics.text: HannoverDv2000
characteristics.characteristic: strain
characteristics.text: Dictyocaulus viviparus HannoverDv2000 cDNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector