characteristics.text: SAMN00164153
characteristics.characteristic: External Id
characteristics.text: LIBEST_009642
characteristics.characteristic: External Id
characteristics.text: Children's Memorial Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.073Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The UI-R-DK0 library is a subtracted library derived from a mixture of five individually tagged normalized rat libraries: brain-nRBP (20%), heart-nRHP (20%), kidney-nRKP (20%), aorta-nRAP (20%), and placenta-nRPP (20%). Each original library was constructed from a mixture of equal amounts of RNA from seven different developmental time-points: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, and adult day 200. (Exception: the aorta pool does not contain embryonic day 17 RNA and the placenta pool contains only the three embryonic stages). Each library was normalized individually according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the DK0 subtracted library, plasmid DNA from each of the five individually tagged normalized libraries was mixed in the proportions specified above and electroporated into competent bacteria for production of single-stranded circular DNA representing the pool of libraries. Single-stranded circular DNA representing these five normalized libraries was then used as a tracer in a subtractive hybridization with a driver (PCR amplified inserts from a plasmid DNA template preparation) comprising: a) a set of about 1,000 arrayed clones from each of the five non-normalized libraries of brain (CT0s), heart (CS0s), kidney (CU0s), aorta (CW0s), and placenta (CX0s). The resulting pool of approximately 5,000 clones represented about 33.3% of the final driver population. A set of about 2,000 arrayed clones from each of the five normalized libraries of brain (CT0), heart (CS0), kidney (CU0), aorta (CW0), and placenta (CX0). The resulting pool of about 10,000 clones represented about 66.6% of the final driver population.
characteristics.characteristic: description
characteristics.text: ADULT
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Sprague-Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: UI-R-DK0
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector