characteristics.text: LIBGSS_000102
characteristics.characteristic: External Id
characteristics.text: SAMN00150948
characteristics.characteristic: External Id
characteristics.text: Stanford University School of Medicine, Palo Alto
characteristics.characteristic: INSDC center name
characteristics.text: 1999-04-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:38:54.140Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: C. parvum (IOWA isolate) genomic DNA was hydrodynamically sheared to produce fragments having a tight size distribution between 1.5 and 3 kb. Adaptors (pGTGACTCA/CAAACCACTGAGTp) were ligated to the randomly sheared gDNA fragments and pAGCTGTTTG linkers were ligated to the Hind III-cleaved M13mp18 vector. The adaptor-containing inserts were annealed and ligated to the vector and transformed into E. coli strain DH125. Recombinant phagemid clones from the first plating of the library were randomly selected for sequence analysis using the M13(-21) forward primer.
characteristics.characteristic: description
characteristics.text: E. coli DH125
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: Hind III
characteristics.characteristic: re_1
characteristics.text: IOWA
characteristics.characteristic: strain
characteristics.text: CpIOWAM13mp18gDNA1
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: M13mp18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0010588
characteristics.characteristic: vector