characteristics.text: LIBEST_023104
characteristics.characteristic: External Id
characteristics.text: SAMN00165681
characteristics.characteristic: External Id
characteristics.text: United States Department of Agriculture-Agriculture Research Service
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:04.723Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: the mRNA was sent to Express Genomics, they synthesized cDNA from the mRNA and inserted it into a plasmid (pExpress-1) using two restriction enzymes Not I and EcoR V. Library description: Normalized cDNA library (Expressed Genomics) from 2nd instar larvae from Panama 95 strain of screwworm. (directionally cloned Normalized cDNA library) After Express Genomics synthesized the cDNA library, the culture was sent to TIGR for sequencing. Library consist of 9387 ESTs. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.
characteristics.characteristic: description
characteristics.text: larvae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000069
characteristics.characteristic: dev-stage
characteristics.text: Cochliomyia hominivorax
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_115425
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRV
characteristics.characteristic: re_2
characteristics.text: Panama 95
characteristics.characteristic: strain
characteristics.text: New World Screwworm Larvae 9387 ESTs
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pExpressed-1
characteristics.characteristic: vector