characteristics.text: LIBEST_019300
characteristics.characteristic: External Id
characteristics.text: SAMN00149953
characteristics.characteristic: External Id
characteristics.text: Institute of Botany, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:15.003Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Jisheng No.1
characteristics.characteristic: cultivar
characteristics.text: Seeds of Leymus chinensis were germinated at 4o under dark conditions and seedlings were grown under experimental field. Leaf of two-month-old plants were harvested and stored at -70o for RNA extraction and cDNA construction. The RNA was extracted with TRIZOL Reagent (GIBCO) and cDNA library was constructed with Creator TM SMART TM cDNA Library Construction Kit (Cat No. K1053-1; BD Biosciences-Clontech). The cDNA was ligated to the Sfi I-digested, dephosphorylated pDNR-LIB vector.
characteristics.characteristic: description
characteristics.text: Two-month-old plants
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Leymus chinensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_52714
characteristics.characteristic: organism
characteristics.text: Sfi I
characteristics.characteristic: re_1
characteristics.text: Sfi I
characteristics.characteristic: re_2
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Leymus chinensis cv. Jisheng No.1 leaf cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector