characteristics.text: LIBEST_015971
characteristics.characteristic: External Id
characteristics.text: SAMN00154125
characteristics.characteristic: External Id
characteristics.text: University of Illinois at Urbana-Champaign
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-03T23:44:05.757Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:19:13.507Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams 82
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: The cDNA library was constructed, normalized and subtracted by the W.M. Keck Center, University of Illinois Urbana-Champaign as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Messenger RNA extracted from Fusarium solani and soybean roots infected with Fusarium solani was converted to ds-cDNA with tagged oligo-dT used to identify the source of the ESTs where possible: ACGCA18(T) = Fusarium solani; TCCGA18(T) = soybean roots infected with Fusarium solani. The ds-cDNAs were size selected (more than 450 bp), adaptored with EcoRI adaptors and digested with NotI. The ds-cDNA were then directionally cloned into EcoRI-NotI digested pBluescriptII SK(+) phagemid vector. The normalized library was subtracted with cDNA synthesized from non-inoculated healthy soybean root mRNA. Clones were sequenced from the 3' end using standard primer M13 reverse n48.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Root and fungal culture
characteristics.characteristic: organ
characteristics.text: Glycine max/Fusarium solani f. sp. glycines mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_283875
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: NSPCC300
characteristics.characteristic: strain
characteristics.text: LI02
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBSIISK(+)
characteristics.characteristic: vector