characteristics.text: SAMN00165871
characteristics.characteristic: External Id
characteristics.text: LIBEST_023298
characteristics.characteristic: External Id
characteristics.text: University of New Hampshire
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:10.417Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: cell type
characteristics.text: The descending aorta from one to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR180 library contains transcripts that were upregulated in SR aortic tissue relative to the corresponding site in the WC aorta. The corresponding WC library (WC180) contains transcripts that were upregulated in the WC aortic tissue relative to SR180.
characteristics.characteristic: description
characteristics.text: XL1 MRF-
characteristics.characteristic: lab-host
characteristics.text: Columba livia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8932
characteristics.characteristic: organism
characteristics.text: BAMHI
characteristics.characteristic: re_1
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: White Carneau
characteristics.characteristic: strain
characteristics.text: Descending Aorta
characteristics.characteristic: tissue-type
characteristics.text: SR180
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006491
characteristics.characteristic: title
characteristics.text: pBluescript SK+
characteristics.characteristic: vector