characteristics.text: SAMN00164481
characteristics.characteristic: External Id
characteristics.text: LIBEST_009981
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:51.690Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1 PCR-based library. Ancylostoma ceylanicum cDNA PCR products of 500-750 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCR-XL-TOPO (Invitrogen) following the cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Vadim Kapulkin from the University of Colorado at Boulder.
characteristics.characteristic: description
characteristics.text: adults
characteristics.characteristic: dev-stage
characteristics.text: TOP10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007796
characteristics.characteristic: lab-host
characteristics.text: Ancylostoma ceylanicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_53326
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Ancylostoma ceylanicum M2 SL1 TOPO Kapulkin
characteristics.characteristic: title
characteristics.text: Plasmid (kanamycin resistant)
characteristics.characteristic: v_type
characteristics.text: pCR-XL-TOPO (Invitrogen)
characteristics.characteristic: vector