characteristics.text: SAMN00166693
characteristics.characteristic: External Id
characteristics.text: LIBEST_023734
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:25.570Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hammond's Dwarf Scarlet
characteristics.characteristic: cultivar
characteristics.text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Complementary DNA (cDNA) was generated from 4.7 micrograms of total RNA from Scarlet runner bean globular-stage suspensor region using a SMART PCR cDNA Synthesis kit (Clontech) with 20 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs). Five micrograms of the Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for sequencing.
characteristics.characteristic: description
characteristics.text: 6-days post-pollination
characteristics.characteristic: dev-stage
characteristics.text: Suspensor Region of Globular-Stage Embryos
characteristics.characteristic: organ
characteristics.text: Phaseolus coccineus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3886
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: Scarlet Runner Bean globular-stage suspensor region
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: TriplEx2
characteristics.characteristic: vector