characteristics.text: SAMN00164765
characteristics.characteristic: External Id
characteristics.text: LIBEST_021021
characteristics.characteristic: External Id
characteristics.text: Children's Memorial Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:06.893Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The CMRC-CP-II1 library is a normalized library (derived from the UI-CP-II0 library) which was normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCTGAATAG. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).
characteristics.characteristic: description
characteristics.text: DH10B T1 phage resistant (Invitrogen)
characteristics.characteristic: lab-host
characteristics.text: Cyanophora paradoxa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2762
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: CMRC-CP-II1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT7T3-Pac (Pharmacia) with a modified polylinker
characteristics.characteristic: vector