characteristics.text: LIBEST_023873
characteristics.characteristic: External Id
characteristics.text: SAMN00166838
characteristics.characteristic: External Id
characteristics.text: Embrapa Genetic Resources and Biotechnology
characteristics.characteristic: INSDC center name
characteristics.text: 2008-11-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:30.320Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: BRA 002747
characteristics.characteristic: cultivar
characteristics.text: Brachiaria brizantha, diploid (2n=2x=18) sexual, cultivated in the field at Embrapa Genetic Resources and Biotechnology was used as plant material to construct the library. Ovaries at stage IV of development (megagametogenesis) were isolated using stereoscope microscope from inflorescences of these plants and immediately frozen in liquid nitrogen after isolation. RNA was extracted using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). Poly(A)+ RNA was extracted from 70 ug of total RNA using Dynabeads (Dynal). Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in pTriplEx2 vector (Clontech, Palo Alto, CA, USA). The plasmids were transformed in Escherichia coli XL1 Blue and white clones were recovered and inserts were sequenced from the 5'-end using PT2F2 PRIMER (5' GCG CCA TTG TGT TGG TAC CC 3').
characteristics.characteristic: description
characteristics.text: Megasporogenesis
characteristics.characteristic: dev-stage
characteristics.text: Ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organ
characteristics.text: Urochloa brizantha
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_240448
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: B105_-IV-
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pTriplex2
characteristics.characteristic: vector