characteristics.text: SAMN00166682
characteristics.characteristic: External Id
characteristics.text: LIBEST_023724
characteristics.characteristic: External Id
characteristics.text: University of Milan
characteristics.characteristic: INSDC center name
characteristics.text: 2009-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:25.150Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Fiesta
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from fruit flesh (a pool of early stage, middle stage and ripening stage fruits) and from young leaves according to Zeng and Yang (Plant Molecular Report 20:417a-417e, 2002). Poly(A)+mRNA extracted from leaves and poly(A)+mRNA from fruit flesh were used to construct a fruit versus leaf subtractive library employing the PCR-Select cDNA Subtraction kit (Clontech), using fruit cDNA as tester and leaf cDNA as driver, to enrich for fruit specific genes. cDNA fragments were cloned into the pGEM-T easy plasmid vector (Promega). The ligated cDNA fragments were transformed into E.coli JM109E host cells.
characteristics.characteristic: description
characteristics.text: JM109E cells
characteristics.characteristic: lab-host
characteristics.text: fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Malus domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3750
characteristics.characteristic: organism
characteristics.text: fruit flesh
characteristics.characteristic: tissue-type
characteristics.text: Md_fruit-leaf_subtractive_cDNA_library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy vector (Promega)
characteristics.characteristic: vector