characteristics.text: LIBEST_009833
characteristics.characteristic: External Id
characteristics.text: SAMN00164337
characteristics.characteristic: External Id
characteristics.text: Lawrence Livermore National Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.090Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCACGCGTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' AND 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
characteristics.characteristic: description
characteristics.text: between 2 weeks - 2 months
characteristics.characteristic: dev-stage
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: pituitary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000007
characteristics.characteristic: tissue-type
characteristics.text: Soares NMAP
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector