characteristics.text: LIBEST_005483
characteristics.characteristic: External Id
characteristics.text: SAMN00160021
characteristics.characteristic: External Id
characteristics.text: Centro de Pesquisas Rene Rachou - FIOCRUZ
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:33.647Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was extracted from cercariae and amplified with the SMART kit (Clonetech) protocol according to the manufacturer's instructions. PCR products were cloned using the TOPO cloning kit (Invitrogen) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: Cercaria
characteristics.characteristic: dev-stage
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: LE
characteristics.characteristic: strain
characteristics.text: Cercaria cDNA obtained by using the SMART PCR kit from Clonetech
characteristics.characteristic: title
characteristics.text: pCR2.1TOPO (Invitrogen)
characteristics.characteristic: vector