characteristics.text: SAMN00152941
characteristics.characteristic: External Id
characteristics.text: LIBEST_021198
characteristics.characteristic: External Id
characteristics.text: Jiangsu Academy of Agricultural Sciences (JAAS)
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:12.043Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Wheat spikes were inoculated with F. graminearum spores at heading stage. Inoculated spikes were harvested 8h, 12h, 24h, 36h, 48h after inoculation. Total RNA and mRNA were extracted by Trizol(Tiangen) and Oligotex mRNA mini kit(Qiagen), respectively. The SSH-cDNA library was conducted by using PCR Select cDNA Subtraction Kit(Clontech company). Poly (A+) RNA from mixed spikes of Sumai3 as tester, mRNA from mixed spikes of Sumai3's susceptible near-isogenic lines as driver. The enriched SSH PCR products were purified and cloned into pMD-18 T vector and transformed to E.coli strain Top10.
characteristics.characteristic: description
characteristics.text: heading stage
characteristics.characteristic: dev-stage
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: Top10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007796
characteristics.characteristic: strain
characteristics.text: F. graminearum inoculated spike
characteristics.characteristic: tissue-type
characteristics.text: SSH-cDNA Library during wheat-Fusarium graminearum interaction in spikes of wheat
characteristics.characteristic: title