characteristics.text: LIBEST_001699
characteristics.characteristic: External Id
characteristics.text: SAMN00156271
characteristics.characteristic: External Id
characteristics.text: Washington University
characteristics.characteristic: INSDC center name
characteristics.text: 1999-04-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:47.797Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nc-1 strain tachyzoites were propagated in human foreskin fibroblasts in vitro. Directional cDNA was synthesized from poly-A mRNAs by oligo dT priming by Takashi Asai, Washington University. CDNAs were cloned into the EcoRI and XhoI sites of Lambda Uni-Zap using reagents and supplies provided by the supplier (Stratagene). Warning: the library contains a small percentage of cDNAs derived from the human host cells.
characteristics.characteristic: description
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Neospora caninum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29176
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Nc-1 Tachyzoite
characteristics.characteristic: strain
characteristics.text: Nc-1Tachyzoite cDNA
characteristics.characteristic: title
characteristics.text: Lambda Uni-Zap
characteristics.characteristic: vector