characteristics.text: SAMN00151878
characteristics.characteristic: External Id
characteristics.text: LIBGSS_000034
characteristics.characteristic: External Id
characteristics.text: San Francisco General Hospital-University of California, San Francisco
characteristics.characteristic: INSDC center name
characteristics.text: 1997-11-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:38:53.880Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: C. parvum (IOWA isolate) genomic DNA was hydrodynamically sheared to produce fragments having a tight size distribution between 2-4 kb by Dr. Yvonne Thorstenson of the Stanford DNA Sequencing and Technology Center (http://sequence-www.stanford.edu/group/techdev/shear.htm). The randomly sheared gDNA was chromatographed on Sephacryl S-400 to remove any small fragments and DNA eluting in the void volume was subcloned into an Hinc II-digested, alkaline phosphatase-treated pGEM3Zf(+) vector and transformed into E. coli strain XL2 Blue MRF'. Recombinant clones from the first plating of the library were selected for sequence analysis using SP6 and T7 primers.
characteristics.characteristic: description
characteristics.text: E. coli XL2 Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: Hinc II
characteristics.characteristic: re_1
characteristics.text: IOWA
characteristics.characteristic: strain
characteristics.text: CpIOWAgDNA3
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM3Zf(+)
characteristics.characteristic: vector