characteristics.text: SAMN00156911
characteristics.characteristic: External Id
characteristics.text: LIBEST_002350
characteristics.characteristic: External Id
characteristics.text: North Carolina State University
characteristics.characteristic: INSDC center name
characteristics.text: 1999-09-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:04.973Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Shoot tips (approx. 2 cm from apex) were collected during the spring, frozen and used for mRNA isolation. The SMART-PCR method (Clontech) was used to prepare a library from 1 ug total RNA, using the Lambda TriplEx vector. Plasmid subclones in pTriplEx were recovered by cre-lox excision in E. coli strain BM25.8 and sequenced from the 5' end.
characteristics.characteristic: description
characteristics.text: E. coli BM25.8
characteristics.characteristic: lab-host
characteristics.text: shoot tips
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000037
characteristics.characteristic: organ
characteristics.text: Pinus taeda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3352
characteristics.characteristic: organism
characteristics.text: SfiI (A)
characteristics.characteristic: re_1
characteristics.text: SfiI (B)
characteristics.characteristic: re_2
characteristics.text: Pine TriplEx shoot tip library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda TriplEx
characteristics.characteristic: vector