characteristics.text: LIBEST_022062
characteristics.characteristic: External Id
characteristics.text: SAMN00153650
characteristics.characteristic: External Id
characteristics.text: The University of Georgia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-11-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:35.340Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Normalized cDNA library was constructed from a single individual of Iris fulva (IF174) collected in Terrebonne Parish, Louisiana. Total RNAs from leaves and roots were isolated with Trizol (Invitrogen). cDNAs were synthesized and normalized with Trimmer-Direct cDNA Normalization Kit (Evrogen). The cDNAs >500 bp in length were used for cDNA library construction. cDNA library was constructed with Creator Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after Sfi I digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Iris fulva
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_92176
characteristics.characteristic: organism
characteristics.text: SfiI A (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: SfiI B (GGCCGAGGCGGCC)
characteristics.characteristic: re_2
characteristics.text: IF174
characteristics.characteristic: strain
characteristics.text: Roots and Leaves
characteristics.characteristic: tissue-type
characteristics.text: Iris fulva EST Library IF1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Clontech pDNR-LIB
characteristics.characteristic: vector