characteristics.text: LIBEST_023002
characteristics.characteristic: External Id
characteristics.text: SAMN00165551
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:02.103Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from dT primed cDNA and cloned into the pDONR222 vector (Invitrogen Corp;, Carlsbad, CA). PolyA RNA was primed with a Biotin-attB2-oligo-dT primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp;, Carlsbad, CA). The second strand was synthesized and an attB1 adaptor was ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCC -3'). cDNA was inserted into the vector using site specific recombination (flanking attB sites on cDNA). Library was constructed in the laboratory of P. Lemaire at Developmental Biology Institute of Marseille and sequencing was performed by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: cleavage to neurula
characteristics.characteristic: dev-stage
characteristics.text: DH10B TonA
characteristics.characteristic: lab-host
characteristics.text: Ciona intestinalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7719
characteristics.characteristic: organism
characteristics.text: attB1
characteristics.characteristic: re_1
characteristics.text: attB2
characteristics.characteristic: re_2
characteristics.text: Roscoff
characteristics.characteristic: strain
characteristics.text: embryos
characteristics.characteristic: tissue-type
characteristics.text: Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)
characteristics.characteristic: title
characteristics.text: pDONR222
characteristics.characteristic: vector