characteristics.text: LIBEST_015716
characteristics.characteristic: External Id
characteristics.text: SAMN00152763
characteristics.characteristic: External Id
characteristics.text: The Plant Biotechnology Institute, National Research Council Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2005-05-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:31.703Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Topas (embryogenic line DH4079)
characteristics.characteristic: cultivar
characteristics.text: Embryogenesis was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Sieving prior to RNA isolation removed the small non-induced microspores. These 3d cultures contained embryogenic as well as pollen-like structures.
characteristics.characteristic: description
characteristics.text: 3d of 32 degrees C heat stress
characteristics.characteristic: dev-stage
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: Sfi IA
characteristics.characteristic: re_1
characteristics.text: Sfi IB
characteristics.characteristic: re_2
characteristics.text: microspore
characteristics.characteristic: tissue-type
characteristics.text: 12JKBNM1
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector