characteristics.text: SAMN00139337
characteristics.characteristic: External Id
characteristics.text: WU_ABGC
characteristics.characteristic: INSDC center name
characteristics.text: 2011-01-31T12:40:03.443Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-03-28T17:13:28.963Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS140120
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS140120
characteristics.characteristic: SRA accession
characteristics.text: Genomic DNA was extracted from 30 *l of blood from 25 unrelated F0 individuals from brown egg layers using a Puregene DNA isolation kit (D-70KA; Gentra Systems, Inc., USA). The RRLs were prepared by digesting 25 *g of pooled DNA using 1,000 units of the restriction enzyme AluI in a total volume of 240 *l. The selection of the restriction enzyme was based on the 10-fold reduction of genome complexity in the optimum size range (100-200 bp) of the sequencing technology platform (Genome Analyzer, Illumina). The digested DNA sample was fractionated on a 10% precast polyacrylamide gel (Biorad) at 100 V for 3 h and stained with ethidium bromide. The size fractions were sliced out of the gel and the DNA was mechanically sheared and and eluted over night in 300 *l recovery buffer (8 mM Tris pH 8.0, 0.08 mM EDTA, 1.25 M ammonium acetate. After a 15-min incubation at 65*C, the eluent was purified using a Montage DNA Gel Extraction Device (Millipore Corporation, Bedford, MA) and precipitated with isopropanol. The DNA was washed with ethanol and re-suspended in DNA hydration solution (Gentra Systems, Inc., USA).
characteristics.characteristic: description
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: brown egg layer
characteristics.characteristic: title