characteristics.text: SAMN00152404
characteristics.characteristic: External Id
characteristics.text: LIBEST_020614
characteristics.characteristic: External Id
characteristics.text: University of Alberta
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:54.907Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Carmen
characteristics.characteristic: cultivar
characteristics.text: Plants were raised in growth chambers with a photoperiod of sixteen hours light and eight hours dark at 24oC and 50% humidity. At the start of the fourth-week following sowing, the outside of the stem above the second internode (counting up from the cotyledons) was scraped directly into a metal sieve over liquid N2. Total RNA was extracted using the Qiagen Maxi Total RNA kit, and polyA+ enriched RNA was obtained from this using Ambion Poly(A)Purist Mag. cDNA synthesis was primed using a NotI-oligodT primer, and using SalI adapters, double-stranded DNA was ligated into SalI NotI sites of pSPORT1.
characteristics.characteristic: description
characteristics.text: 4 weeks post-sowing
characteristics.characteristic: dev-stage
characteristics.text: stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: organ
characteristics.text: Cannabis sativa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3483
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: cortical regions including cambium, phloem, bast fibers
characteristics.characteristic: tissue-type
characteristics.text: stem phloem (bast) fiber enriched library CS01
characteristics.characteristic: title
characteristics.text: pSPORT 1
characteristics.characteristic: vector