characteristics.text: SAMN00115074
characteristics.characteristic: External Id
characteristics.text: Computational Biology Research Center, AIST
characteristics.characteristic: INSDC center name
characteristics.text: 2010-10-07T22:48:35.753Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:59:48.047Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS116607
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS116607
characteristics.characteristic: SRA accession
characteristics.text: Immunoprecipitation was performed using anti-Aub and anti-AGO3 (polyclonal) antibodies immobilized on Dynabeads Protein G (Invitrogen). Immunoprecipitation buffer contained 50 mM Tris-HCl (pH8.0), 150 mM NaCl, 1 mM dithiothreitol (DTT), 0.1% SDS, 1% Triton-X, 0.5% sodium deoxycholate, 2μg/ml pepstatin, 2μg/ml leupeptin, and 0.5% aprotinin. Approximately 1500 testes were used per immunoprecipitation. The reaction mixtures were rocked at 4*C for 1 h, the bead fractions were washed five times with binding buffer and the protein pools bound to the beads were eluted with SDS-PAGE sample buffer. After heating to 95*C for 5 min, protein samples were run on SDS-PAGE gels and processed for western blot analysis.
characteristics.characteristic: description
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: AGO3-associated small RNAs extracted from fly testes
characteristics.characteristic: title