characteristics.text: LIBEST_009715
characteristics.characteristic: External Id
characteristics.text: SAMN00164221
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2001-08-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:44.903Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Extra-radical mycelium from Glomus intraradices grown in a two-compartment petri-plate system in association with Daucus carota transformed roots. M medium containing 35 micromolar potassium phosphate was added to the extra-radical mycelium 12 hours prior to harvest. cDNA was prepared from total RNA using the Clontech Smart cDNA synthesis system. The cDNA was directionally ligated into Lambda TriplEX2 vector (Clontech) and packaged using Gigapack Gold packaging extracts. Plasmids (pTriplEX2) containing cDNA inserts were obtained from the recombinant lambda TriplEX2 phage via Cre-lox mediated conversion in BM25.8 cells (Clontech).
characteristics.characteristic: description
characteristics.text: Rhizophagus intraradices
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4876
characteristics.characteristic: organism
characteristics.text: Extra-radical mycelium
characteristics.characteristic: tissue-type
characteristics.text: Glomus intraradices extra-radical mycelium
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Lambda TriplEX2
characteristics.characteristic: vector