characteristics.text: LIBEST_022999
characteristics.characteristic: External Id
characteristics.text: SAMN00165548
characteristics.characteristic: External Id
characteristics.text: USDA-ARS-BARC
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:02.043Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Merton Thornless
characteristics.characteristic: cultivar
characteristics.text: Rubus F2 backcross to F1 of R. ulmifolius (R. rusticanus) inermis x R. thyrsiger. The tetraploid blackberry cultivar, Merton Thornless (PI 553276), was selected for EST development, because it is the source of the thornless trait in many commercially grown thornless cultivars. Young expanding leaves of 'Merton Thornless' were harvested in July, 2004, from plants growing on the North Farm of the Beltsville Agricultural Research Center. No enrichment was done of the total RNA extracted before mRNA was separated from total RNA. The cDNA library was cloned in the Gateway system (Invitrogen Corp., Carlsbad, Calif.) with the pDONR222 vector and ElectroMAX DH10B T1 Phage Resistant Cells (Invitrogen Corp.). Kanamycin was used to select colonies containing vectors. The M13F primer was used to sequence from the 5' end of 3884 clones.
characteristics.characteristic: description
characteristics.text: Newly emerging
characteristics.characteristic: dev-stage
characteristics.text: DH 10B
characteristics.characteristic: lab-host
characteristics.text: Rubus ulmifolius var. inermis x Rubus thyrsiger
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_519942
characteristics.characteristic: organism
characteristics.text: sfiI
characteristics.characteristic: re_1
characteristics.text: sfiII
characteristics.characteristic: re_2
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: RH_MEa blackberry emerging leaf
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDONR222
characteristics.characteristic: vector