characteristics.text: LIBEST_000646
characteristics.characteristic: External Id
characteristics.text: SAMN00155245
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1997-03-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:16.463Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Toxoplasma gondii parasites were grown in vitro in cultures of human foreskin fibroblasts under conditions that induce tachyzoite-bradyzoite differentiation. The library was constructed by A. Hehl and I. Manger, Stanford University. Directional cDNA was synthesized by oligo d(T) priming and cloned into EcoRI and XhoI sites of the Lambda ZAP vector using the ZAP-cDNA Synthesis Kit (Stratagene). WARNING: Library contains A SMALL PERCENTAGE OF human cDNAs derived from the host cells
characteristics.characteristic: description
characteristics.text: in vitro Bradyzoite, 6day
characteristics.characteristic: dev-stage
characteristics.text: XL 1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: ME49
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006645
characteristics.characteristic: strain
characteristics.text: TgME49 invivo Bradyzoite cDNA
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP
characteristics.characteristic: vector