characteristics.text: SAMN00155005
characteristics.characteristic: External Id
characteristics.text: LIBEST_000393
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 1996-01-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:10.150Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Double stranded cDNA was prepared by PCR amplification of first strand, oligo(dT) primed cDNA prepared from poly(A) plus RNA from day fourteen post-infection fourth stage larvae isolated from the jird host. Unamplified titer: 6.4 x 105 pfu/ml; Amplified titer: 2 x 109 pfu/ml; Background: < 6% non-recombinants; Average insert size: ~ 700 bp; Transcripts: 98 % full length transcripts; Primers used in library construction: 5' end: XSL: 5' GCT CTA GAG CGG TTT AAT TAC CCA AGT TTG AG 3' 3' end: XET: 5' GCG CGC TCG AGA ATT CTT TTT TTT TTT TTT TTT TTT 3'. The library was made by Inca Ghosh, Nithyakalyani Raghavan, Ward Eisinger and Alan Scott (email ascott@hpcsun01.sph.jhu.edu).
characteristics.characteristic: description
characteristics.text: day fourteen post-infection fourth stage larvae
characteristics.characteristic: dev-stage
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Xba I (5' end)
characteristics.characteristic: re_1
characteristics.text: Eco RI (3' end)
characteristics.characteristic: re_2
characteristics.text: TRS Labs
characteristics.characteristic: strain
characteristics.text: Brugia malayi L4 JHU93SL-BmL4
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Uni-Zap XR
characteristics.characteristic: vector