characteristics.text: LIBEST_021869
characteristics.characteristic: External Id
characteristics.text: SAMN00154511
characteristics.characteristic: External Id
characteristics.text: The University of Melbourne
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:29.603Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA isolated from tammar wallaby mammary gland during lactation (days 55, 87, 130, 180, 220, 260) and involution (5 days after removal of pouch young at 45d lactation) were pooled and used for normalised library construction. Subsequent cDNA molecules were directionally cloned into pMCVSport6-ccdB vector using NotI (3') and EcoRV (5') sites. Quality control of the resulting library revealed a titre of 1.2 x 10e5 cfu/ml with a mean insert size of 1.2kb. Southern hybridisation for abundance of alpha-casein cDNA molecules revealed a reduction of 37 fold (7.8% in the non-normalised library, 0.2% in the normalised library).
characteristics.characteristic: description
characteristics.text: mix of mammary tissues from day 55,87,130,180,220,260 of lactation and 5 days involution after 45 days of lactation
characteristics.characteristic: dev-stage
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: organ
characteristics.text: Notamacropus eugenii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9315
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRV
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: tissue-type
characteristics.text: normalized library from a mix of tammar wallaby mammary tissues
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMCVSport6-ccdB
characteristics.characteristic: vector