characteristics.text: SAMN00154055
characteristics.characteristic: External Id
characteristics.text: LIBEST_015074
characteristics.characteristic: External Id
characteristics.text: University of New Mexico
characteristics.characteristic: INSDC center name
characteristics.text: 2004-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:12.573Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was harvested from rediae, cercariae, metacercariae, adults and 48-hour cultivated sporocysts of E. paraensei. cDNA was generated using oligo-dT primers. The sporocyst cDNA was subtracted against the combined non-sporocyst cDNA using the PCR-Select Suppressive Subtraction Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and sequenced on both strands using plasmid-specific primers (M13 rev and M13 -20).
characteristics.characteristic: description
characteristics.text: Biomphalaria glabrata
characteristics.characteristic: lab-host
characteristics.text: whole body, sporocyst
characteristics.characteristic: organ
characteristics.text: Echinostoma paraensei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_48215
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: E. paraensei sporocyst SSH library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector