characteristics.text: SAMN00166342
characteristics.characteristic: External Id
characteristics.text: LIBEST_023448
characteristics.characteristic: External Id
characteristics.text: Indian Institute of Technology, Kharagpur
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:15.330Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: JRC 321
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from fibre forming stage of jute stem by hot borate method and purified by isopycnic CsCl density gradient ultracentrifugation. Poly (A+) mRNA was isolated using oligo (dT) cellulose. Double-strand cDNA was synthesized using the SMARTTM cDNA Library Construction Kit (Clontech Laboratories Inc., Palo Alto, CA, USA) following the manufacturer's instruction. The double stranded cDNA synthesized was size fractionated using Sephacryl S-300. Each of the fractions was then used to generate cDNA library. The library was hybridized with double stranded cDNA probe generated from poly(A)+mRNA of non-fibre forming stage of jute stem. After three round of hybridization, unhybridized clones were selected. The selected clones were then subjected to single pass sequencing. The resultant sequences were subjected to VecScreen for removing the vector sequences. The clones were putatively identified by searching against plant database in TIGR using tblastx and tblastN. Clones with e-value greater than 10-10 and sequence similarity of more than 95% were considered significant.
characteristics.characteristic: description
characteristics.text: 20-45 days after sowing
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: organ
characteristics.text: Corchorus capsularis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_210143
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Bast fiber and associated tissues
characteristics.characteristic: tissue-type
characteristics.text: Jute Plasmid library 20-45 days after sowing
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR LIB
characteristics.characteristic: vector