characteristics.text: SAMN00156281
characteristics.characteristic: External Id
characteristics.text: LIBEST_001709
characteristics.characteristic: External Id
characteristics.text: University of Texas Southwestern Medical Center at Dallas
characteristics.characteristic: INSDC center name
characteristics.text: 1999-04-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:48.100Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: syncytiotrophoblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000371
characteristics.characteristic: cell type
characteristics.text: Freshly isolated cytotrophoblast cells from human mid-term placenta were cultured in DMEM containing 2% serum for 4 days and differentiated into syncytiotrophoblast. Cytotrophoblast double-strand cDNAs were used as a driver, and syncytiotrophoblast double-strand cDNA as a tester. The clones from subtracted syncytiotrophoblast cDNA library are expressed at high level in syncytiotrophoblast, and not expressed or expressed at low level in cytotrophoblast cells.
characteristics.characteristic: description
characteristics.text: cytotrophoblast cells from mid-term placenta
characteristics.characteristic: dev-stage
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: placenta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001987
characteristics.characteristic: tissue-type
characteristics.text: Subtracted syncytiotrophoblast cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT-Adv
characteristics.characteristic: vector