characteristics.text: SAMN00000797
characteristics.characteristic: External Id
characteristics.text: University of Tokyo, Graduate School of Frontier Science
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-05T09:30:29.413Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:43:00.850Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS001146
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS001146
characteristics.characteristic: SRA accession
characteristics.text: HT-29
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001193
characteristics.characteristic: cell-line
characteristics.text: HT-29 cells were cultured in RPMI1640 or McCoy's 5A medium supplemented with 10% fetal bovine serum. Cells were incubated with PBS for 72 h;the culture medium was replaced with fresh medium plus PBS 24 h after initiation of incubation.Total RNA for 5'SAGE analysis and for RT-PCR was extracted using RNA-Bee. The quality of total RNA from each cell line was analyzed using an Agilent 2100 Bioanalyzer.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Generic sample from Homo sapiens
characteristics.characteristic: title