characteristics.text: SAMN00166098
characteristics.characteristic: External Id
characteristics.text: LIBEST_015624
characteristics.characteristic: External Id
characteristics.text: University of Delaware Graduate College of Marine Studies
characteristics.characteristic: INSDC center name
characteristics.text: 2004-04-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-27T21:01:11.157Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: toxic zoospore
characteristics.characteristic: cell type
characteristics.text: A serial analysis of gene expression (SAGE) library was constructed from toxic Pfiesteria shumwayae zoospores. RNA was reverse transcribed with an oligo dT - Heel primer where the Heel is a 20 base extension at the 5' end of the primer. The 3' end cDNA sequences for tags of interest were determined by amplification of cDNA using SAGE tag-specific forward primers and the Heel reverse primer. PCR products were then cloned into pCR4 TOPO-TA cloning vector and individual clones were sequenced.
characteristics.characteristic: description
characteristics.text: Pfiesteria shumwayae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410738
characteristics.characteristic: organism
characteristics.text: Pfiesteria shumwayae EST sequences from SAGE tags
characteristics.characteristic: title
characteristics.text: pCR4 TOPO-TA
characteristics.characteristic: vector