characteristics.text: LIBEST_000983
characteristics.characteristic: External Id
characteristics.text: SAMN00155574
characteristics.characteristic: External Id
characteristics.text: The University of York
characteristics.characteristic: INSDC center name
characteristics.text: 1997-08-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:27.847Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total mRNA was isolated from lung stage worms and the cDNA expression library was constructed using the Lambda Zap cDNA cloning system (Stratagene-Uni-Zap XR), the cDNA being directionally cloned between the EcoRI and the XhoI sites of the Bluescript phagemid. Bluescript phagemid was excised by mass in vivo excision of the library and inserted into E.coli XL1 Blue. The library was prepared by Richard Harrop, Department of Biology, University of York, York. YO1 5YW.
characteristics.characteristic: description
characteristics.text: XL1 Blue
characteristics.characteristic: lab-host
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Puerto Rican
characteristics.characteristic: strain
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: tissue-type
characteristics.text: SmL
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Bluescript
characteristics.characteristic: vector