characteristics.text: LIBEST_017820
characteristics.characteristic: External Id
characteristics.text: SAMN00166248
characteristics.characteristic: External Id
characteristics.text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry.
characteristics.characteristic: INSDC center name
characteristics.text: 2005-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:35.833Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Clontech PCR-Select cDNA Subtraction kit (BD Sciences) was used to make a library of cDNA clones differentially expressed during F. graminearum infection of wheat heads. 10 infected heads were collected daily for 13 days and pooled for RNA extraction, control plants were treated like infected plants, except these were mock 'inoculated' with water only. For the subtraction process, 2 ug of PolyA+ RNA was used for each tester (infected tissue) and driver (control tissue) samples, according to the manufacturer's protocol (BD Sciences). Products from secondary PCR were cloned into the pGEM-T Easy vector system (Promega) according to manufacturer's instructions, and transformed into TOP10 cells (Invitrogen), also according to manufacturer's instruction. Constructed by Agnieszka M. Mudge.
characteristics.characteristic: description
characteristics.text: From anthesis to grain fill
characteristics.characteristic: dev-stage
characteristics.text: E. coli TOP10
characteristics.characteristic: lab-host
characteristics.text: Heads
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000033
characteristics.characteristic: organ
characteristics.text: Fusarium graminearum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5518
characteristics.characteristic: organism
characteristics.text: CS3040
characteristics.characteristic: strain
characteristics.text: Subtracted cDNA library from Fusarium graminearum infected wheat heads (sFHB)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector