characteristics.text: SAMN00165588
characteristics.characteristic: External Id
characteristics.text: LIBEST_023023
characteristics.characteristic: External Id
characteristics.text: University of New Hampshire
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:02.710Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Smooth Muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001135
characteristics.characteristic: cell type
characteristics.text: Primary Culture, No Sub-Culture
characteristics.characteristic: cell-line
characteristics.text: One to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR143 library contains transcripts that were upregulated in SR SMC relative to WC. The corresponding WC library (WC143) contains transcripts that were upregulated in the WC SMC relative to SR143.
characteristics.characteristic: description
characteristics.text: XL1 MRF-
characteristics.characteristic: lab-host
characteristics.text: Columba livia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8932
characteristics.characteristic: organism
characteristics.text: BAMHI
characteristics.characteristic: re_1
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Show Racer
characteristics.characteristic: strain
characteristics.text: Descending Aorta
characteristics.characteristic: tissue-type
characteristics.text: SR143
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006491
characteristics.characteristic: title
characteristics.text: pBluescript SK+
characteristics.characteristic: vector