characteristics.text: SAMN00152959
characteristics.characteristic: External Id
characteristics.text: LIBEST_021217
characteristics.characteristic: External Id
characteristics.text: Biomedical Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:12.537Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly(A)+ RNA was isolated from the hepatopancreas of unexposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI and EcoRI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector obtained from Stratgene. Mass excision of the unamplified libraries was performed and recombinant phagemids were stored in 96-well format ordered arrays.
characteristics.characteristic: description
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: BS-90
characteristics.characteristic: strain
characteristics.text: Biomphalaria glabrata resistant snail (BS-90) hepatopancreas library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector