characteristics.text: LIBEST_005520
characteristics.characteristic: External Id
characteristics.text: SAMN00160058
characteristics.characteristic: External Id
characteristics.text: Genoscope - Centre National de Sequencage
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:34.940Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Jemalong
characteristics.characteristic: cultivar
characteristics.text: M. truncatula sterilised seeds were germinated for 72h at 25 C, before transplanting into a 1/3 Epoisses soil : 2/3 calcined Terragreen mix in the presence of onion root fragments colonized by the arbuscular mycorrhizal fungus Glomus intraradices (Schenck & Smith, isolate LPA8). The plants were watered every day and twice a week with a modified nutrient Long Ashton solution without phosphate but with a high level of nitrate. After 3 weeks RNA was extracted from whole root systems. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France). Note : EST may be of fungal origin.
characteristics.characteristic: description
characteristics.text: harvested 3 weeks post inoculation with Glomus intraradices
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: arbuscular mycorrhiza
characteristics.characteristic: tissue-type
characteristics.text: MtBC
characteristics.characteristic: title
characteristics.text: pBluescript pSK
characteristics.characteristic: vector