characteristics.text: LIBEST_019635
characteristics.characteristic: External Id
characteristics.text: SAMN00150262
characteristics.characteristic: External Id
characteristics.text: Institut Pasteur de Lille
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:23.683Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was harvested from Echinostoma caproni infected B. glabrata at 24h, 48h and 96h post exposure, and from control snails at 24h, 48h and 96h after sham exposure. Combined cDNAs from parasite exposed and unexposed snails were subtracted using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR4(r)-TOPO(r) (Invitrogen, Carlsbad, CA) and sequenced using the forward plasmid-specific primer T7
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Whole body
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001489
characteristics.characteristic: organ
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Bg.CB
characteristics.characteristic: strain
characteristics.text: B. glabrata SSH EST library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR4(r)-TOPO(r)
characteristics.characteristic: vector