characteristics.text: LIBEST_022968
characteristics.characteristic: External Id
characteristics.text: SAMN00165513
characteristics.characteristic: External Id
characteristics.text: School of Life Sciences, Xiamen University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:00.563Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Female Sebastiscus marmoratus weighing 23-27 g, were captured from coastal area in Xiamen city, Fujian province, China. Before the exposure experiment, the fish were acclimated in tanks (25 fish in each tank) containing 50 L of aerated sand-filtered seawater, with natural photoperiod for 7 days. Fish were dosed via single intraperitoneal injection with PCBs (3 mg/kg body weight in corn oil) or with corn oil as vehicle controls. After injection for 3 days, fish were randomly sampled from each group. The isolated brains were frozen in liquid N2 immediately stored at -80o until analyzed. For the SSH procedure, mRNA was isolated from brains and two libraries constructed in pMD18-T vector, enriched for genes up- (forward subtracted) or down-regulated (reverse subtracted), respectively in response to PCBs.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E.coli TOP10
characteristics.characteristic: lab-host
characteristics.text: Brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Sebastiscus marmoratus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_41446
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Suppression subtractive hybridization libraries for brain of Sebastiscus marmoratus in response to PCBs
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMD-18T
characteristics.characteristic: vector