characteristics.text: LIBEST_021378
characteristics.characteristic: External Id
characteristics.text: SAMN00153099
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:16.910Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Solanum lycopersicum plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from the most apical leaves of 5 week old Solanum lycopersicum plants. Trichomes were harvested by brushing leaves frozen using liquid nitrogen and separated from contaminating leaf fragments by passing the frozen material through a sieve. RNA was isolated from the trichomes using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.
characteristics.characteristic: description
characteristics.text: Apical internodes of 5-week old plants.
characteristics.characteristic: dev-stage
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: Sfi I A (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: Sfi I B (GGCCGCCTCGGCC)
characteristics.characteristic: re_2
characteristics.text: Isolated leaf trichomes
characteristics.characteristic: tissue-type
characteristics.text: Solanum lycopersicum trichomes
characteristics.characteristic: title
characteristics.text: pDNR_lib (Clontech)
characteristics.characteristic: vector