characteristics.text: SAMN00150070
characteristics.characteristic: External Id
characteristics.text: LIBEST_019438
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:18.573Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Nippostrongylus brasiliensis activated L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of Imperial College London, UK.
characteristics.characteristic: description
characteristics.text: L3, activated
characteristics.characteristic: dev-stage
characteristics.text: GC10 cells
characteristics.characteristic: lab-host
characteristics.text: Nippostrongylus brasiliensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_27835
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: N.brasiliensis_EST_L3A_SL1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector