characteristics.text: SAMN00161791
characteristics.characteristic: External Id
characteristics.text: LIBEST_007261
characteristics.characteristic: External Id
characteristics.text: Clemson University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-12-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:31.110Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Morex
characteristics.characteristic: cultivar
characteristics.text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 90% RH for 24 hr. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)
characteristics.characteristic: description
characteristics.text: TJC121
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: Seedling shoot
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000992
characteristics.characteristic: tissue-type
characteristics.text: Hordeum vulgare seedling shoot EST library HVcDNA0002 (Dehydration stress)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: lambdaZAP
characteristics.characteristic: vector