characteristics.text: LIBEST_012564
characteristics.characteristic: External Id
characteristics.text: SAMN00153941
characteristics.characteristic: External Id
characteristics.text: University of Perugia
characteristics.characteristic: INSDC center name
characteristics.text: 2003-01-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:01.003Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B17 x P13 F1 population
characteristics.characteristic: cultivar
characteristics.text: This EST was obtained with the cDNA-AFLP method (Bachem et al.Plant Journal 9:745-753, 1996). Total RNA was extracted from three female fertile and thr ee female sterile alfalfa plants from an F1 population obtained by crossing th e plants B17 and P13 (Rosellini et al. Theoretical and Applied Genetics, 97:1 289- 1295, 1998). Equal amounts of RNA from each fertile or sterile plant were b ulked and used for the cDNA-AFLP protocol. The restriction endonucleases EcoRI an d MseI were used. Bands that were polymorphic between the female sterile and female fertile bulks were isolated from the polyacrilamide gel, cloned in a plasmid vector (Invitrogen PCR4 TOPO) and sequenced using the M13F primer. The published sequence begins and ends with the EcoRI (GAATTC) and MseI (TTAA) recognition sequences, respectively. The technical help of Francesco Panara is gratefully acknowledged.
characteristics.characteristic: description
characteristics.text: From ovule primordia to anthesis
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Developing flower buds
characteristics.characteristic: organ
characteristics.text: Medicago sativa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3879
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Alfalfa developing flower bud library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Invitrogen PCR4 TOPO Vector
characteristics.characteristic: vector