characteristics.text: LIBEST_009982
characteristics.characteristic: External Id
characteristics.text: SAMN00164482
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:51.713Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Toxoplasma RH strain tachyzoites were grown in human foreskin fibroblast cultures in vitro. The library was originally constructed by K.L.Wan, Cambridge University. cDNAs were synthesized from polyA RNAs by oligo d(T) priming and directionally cloned into the EcoRI to XhoI sites of the Lambda ZapII vector using the ZAP-cDNA synthesis kit (Stratagene). The primary cDNA library was mass excised as phagemid using ExAssist helper phage (Stratagene). Phagemid DNA was extracted by phenol-chloroform method, and hybridized against a pool of over-represented ESTs (N>=12, from 5596 previous reads). The subtracted library was electroporated into DH10B (GeneHog, Invitrogen, Inc). WARNING: the library contains a small percentage of cDNAs derived from the human host cells. Library Scource: David Sibley, Washington University.
characteristics.characteristic: description
characteristics.text: Tachyzoite
characteristics.characteristic: dev-stage
characteristics.text: DH10B (GeneHog, Invitrogen, Inc.)
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: RH (Type I)
characteristics.characteristic: strain
characteristics.text: TgRH Tachyzoite Subtracted cDNA Library
characteristics.characteristic: title
characteristics.text: pBluscript SK-
characteristics.characteristic: vector