characteristics.text: SAMN00150567
characteristics.characteristic: External Id
characteristics.text: LIBEST_019975
characteristics.characteristic: External Id
characteristics.text: The University of Arizona
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:33.400Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B73
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007022
characteristics.characteristic: cultivar
characteristics.text: Maize Full length cDNA library constructed by Invitrogen from multiple tissues: 7 day seedling, 7 day seedling with 2 day salt treatment, 4 hour 45 C heat shock, 24 hour 10 C treatment, 4 hour UV-B treatment, 2 day osmotic stress, 7 day etiolated seedling and Juvenile leaf. Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources.
characteristics.characteristic: description
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: dev-stage
characteristics.text: DH10B T1 phage resistant
characteristics.characteristic: lab-host
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: tissue-type
characteristics.text: ZM_BFc
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMV.SPORT-6.1
characteristics.characteristic: vector