characteristics.text: LIBEST_023003
characteristics.characteristic: External Id
characteristics.text: SAMN00165552
characteristics.characteristic: External Id
characteristics.text: Hokkaido University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:02.123Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ultra
characteristics.characteristic: cultivar
characteristics.text: Preemergent proteoid root zones of Lupinus albus (white lupin) grown under phosphate deficient conditions were harvested, and poly(A)+ RNA was isolated. Seven micrograms of poly(A)+ RNA obtained was used for construction of an early proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.
characteristics.characteristic: description
characteristics.text: preemergent proteoid root zones, phosphate-stressed
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: preemergent proteoid roots, phosphate-stressed (cluster root)
characteristics.characteristic: organ
characteristics.text: Lupinus albus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3870
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Preemergent cluster root ESTs derived from phosphate-stressed Lupinus Albus
characteristics.characteristic: title
characteristics.text: pBluescript SK(+/-)
characteristics.characteristic: vector