characteristics.text: SAMN00152528
characteristics.characteristic: External Id
characteristics.text: LIBEST_020749
characteristics.characteristic: External Id
characteristics.text: College of Veterinary Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:58.823Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from pools of adult females and males, separately, and suppression subtractive hybridization (SSH) was performed by using the PCR-Select cDNA Subtraction kit (Clontech): the forward subtracted library was constructed by using females as tester and males as driver, and the reverse-subtracted library was constructed by using males as tester and females as driver. Forward library was ligated into pGEM-T easy vector. Recombinant plasmids were transformed into competent Escherichia coli cells.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Culex pipiens pallens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_42434
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Shenzhen
characteristics.characteristic: strain
characteristics.text: sex-differentially expressed cDNA library for Culex pipiens pallens
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type