characteristics.text: SAMN00164283
characteristics.characteristic: External Id
characteristics.text: LIBEST_009777
characteristics.characteristic: External Id
characteristics.text: Duke University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:46.493Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Stress condition II library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells grown to mid-log phase in TAP (NH4+ - containing) and shifted to TAP - NO3- (24hrs); H2 production conditions (0, 12hr, 24hr) see Melis et al.,(2000) Plant Phys. 122: 127-135; TAP + H2O2 (1, 12, 24 hr); TAP + sorbitol (1, 2, 6, 24 hr); TAP + Cd (1, 2, 6, 24 hr). PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al., (1996) Genome Research 6: 791-806.
characteristics.characteristic: description
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: CC-1690 wild type mt+ 21gr
characteristics.characteristic: strain
characteristics.text: C. reinhardtii CC-1690, Stress II (normalized), Lambda Zap II
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK-
characteristics.characteristic: vector