characteristics.text: LIBEST_020244
characteristics.characteristic: External Id
characteristics.text: SAMN00150812
characteristics.characteristic: External Id
characteristics.text: University of Bern
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:42.717Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM39 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.
characteristics.characteristic: description
characteristics.text: Trophozoite
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002592
characteristics.characteristic: dev-stage
characteristics.text: UAM39
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: lab-host
characteristics.text: Plasmodium falciparum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5833
characteristics.characteristic: organism
characteristics.text: Blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Ugandan isolate UAM39 Pf var gene cDNA
characteristics.characteristic: title
characteristics.text: pcrII-TOPO
characteristics.characteristic: vector