characteristics.text: LIBEST_022151
characteristics.characteristic: External Id
characteristics.text: SAMN00166300
characteristics.characteristic: External Id
characteristics.text: Colorado State University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-11-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.397Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Morex
characteristics.characteristic: cultivar
characteristics.text: Seeds were micromalted at 14oC for 48 h (steeping), 20oC for 96 h, kilning steps consisting of 55oC for 3 h, 60oC for 5 h, 65oC for 5 h, 70oC for 3 h, 75oC for 2 h, 85oC for 3 h, and 90oC for 1 h. Malted seeds were collected after 96h germination at 2oC. SSH was performed between cDNA populations from dry seed (Driver) and malted seed (Tester). Forward PCR-based subtraction was performed (Tester minus Driver) and the amplification products ligated into the pTAdvantage PCR cloning vector (BD Biosciences-Clontech, Palo Alto, CA). E. coli was transformed with this ligation reaction by electroporation.
characteristics.characteristic: description
characteristics.text: malted seed (day 4 germination 96 h germination, 20oC)
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: seed
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009010
characteristics.characteristic: tissue-type
characteristics.text: MaltedMorex
characteristics.characteristic: title
characteristics.text: pTAdvantage PCR cloning vector
characteristics.characteristic: vector