characteristics.text: LIBEST_012436
characteristics.characteristic: External Id
characteristics.text: SAMN00153928
characteristics.characteristic: External Id
characteristics.text: UMR INRA/Universite Henri Poincare 1136
characteristics.characteristic: INSDC center name
characteristics.text: 2003-06-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:57.990Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Eucalyptus globulus ssp. bicostata Kirkp.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after four days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).
characteristics.characteristic: description
characteristics.text: four-days-old
characteristics.characteristic: dev-stage
characteristics.text: ectomycorrhiza
characteristics.characteristic: organ
characteristics.text: Eucalyptus globulus subsp. bicostata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_71272
characteristics.characteristic: organism
characteristics.text: Eucalyptus globulus cDNA after four days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector