characteristics.text: SAMN00153568
characteristics.characteristic: External Id
characteristics.text: LIBEST_021967
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-10-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:31.997Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Glandular-haired alfalfa plants were established in green house in 40 half gallon pots. Plants were grown in Metromix 350 and fertilized with MiracleGro as needed. They were cut back closely to encourage the emergence of vigorous shoots. Trichomes were isolated from stems, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown. With minimal handling of the stem, the apical bud, leaves and nodes were discarded, and the trichomes isolated from the internode segments. The cDNA library from alfalfa trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA.
characteristics.characteristic: description
characteristics.text: Trichome from the stem, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown
characteristics.characteristic: dev-stage
characteristics.text: Medicago sativa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3879
characteristics.characteristic: organism
characteristics.text: Sfi IA (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: Sfi IB (GGCCGCCTCGGCC)
characteristics.characteristic: re_2
characteristics.text: Glandular trichomes isolated from stem
characteristics.characteristic: tissue-type
characteristics.text: Alfalfa (Medicago sativa) Trichome isolated from the stem
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector