characteristics.text: SAMN00016559
characteristics.characteristic: External Id
characteristics.text: University of Washington Genome Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-11T12:26:56.440Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:57:50.027Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS084547
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS084547
characteristics.characteristic: SRA accession
characteristics.text: A collection of Human Yap65 variants were obtained using degenerate chemical synthesis. Following a short PCR amplification to yield double-stranded DNA molecules, variants were cloned into the T7 phage packaging extract for phage display. Phage displaying hYap65 variants were submitted to six rounds of selection for binding to the cognate peptide ligand. Illumina sequencing libraries were prepared from phage of the unselected (Input) library, as well as after the third (Round III) and sixth rounds (Round VI) of selection. 76bp paired-end reads were acquired from each library using the Illumina GAII platform.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Generic sample from Homo sapiens
characteristics.characteristic: title