characteristics.text: LIBEST_023598
characteristics.characteristic: External Id
characteristics.text: SAMN00166491
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:19.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: growth in rich medium ( 2% malt extract, 2% glucose, 0.5% peptone) for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: Postia placenta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_104341
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: MAD 698
characteristics.characteristic: strain
characteristics.text: CAIO Postia placenta in rich medium
characteristics.characteristic: title
characteristics.text: p21_cDNA
characteristics.characteristic: vector