characteristics.text: LIBEST_011113
characteristics.characteristic: External Id
characteristics.text: SAMN00153884
characteristics.characteristic: External Id
characteristics.text: Princeton University
characteristics.characteristic: INSDC center name
characteristics.text: 2002-09-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:22.583Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Stromal cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000499
characteristics.characteristic: cell type
characteristics.text: AFT024
characteristics.characteristic: cell-line
characteristics.text: Two directionally cloned cDNA libraries were made from a hematopoietic stem cell-supporting stromal cell line (AFT024) and from a non-supporting stromal cell line (2018). Subtractive hybridization was performed by hybridization of the target, AFT024, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, 2018 cDNA library in pSport2 with inserts cloned in the complementary orientation. The AFT024-subtracted library contains 4.2x105 clones and is depleted of common housekeeping gene products eg. beta-actin and enriched for transcripts specific to AFT024. For detailed protocols and additional information please see our website at http://stromalcell.princeton.edu.
characteristics.characteristic: description
characteristics.text: Embryonic day 14-14.5
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Fetal Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: C57Bl/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Fetal Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: tissue-type
characteristics.text: AFT024-subtracted library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Sport 1
characteristics.characteristic: vector