characteristics.text: SAMN00164554
characteristics.characteristic: External Id
characteristics.text: LIBEST_022319
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-06-04T21:03:09.337Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: Solid culture media, BCDA media. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: Asexual stage, monoic organism
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Physcomitrium patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Villersexel 2003
characteristics.characteristic: strain
characteristics.text: Protonema
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030003
characteristics.characteristic: tissue-type
characteristics.text: CAYY Physcomitrella patens subsp. patens Villersexel - regular (H)
characteristics.characteristic: title
characteristics.text: pMCL200_cDNA
characteristics.characteristic: vector