characteristics.text: LIBEST_001306
characteristics.characteristic: External Id
characteristics.text: SAMN00155890
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 1998-05-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:37.227Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B73
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007022
characteristics.characteristic: cultivar
characteristics.text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer. The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with XhoI and size-selected. The resulting molecules were directionally cloned into the EcoRI and XhoI sites of the HybriZAP lambda vector (Stratagene) and excised as pAD-GAL4 phagemids.
characteristics.characteristic: description
characteristics.text: Two-leaf-stage green seedling
characteristics.characteristic: dev-stage
characteristics.text: XL1-MFR Blue
characteristics.characteristic: lab-host
characteristics.text: green seedlings
characteristics.characteristic: organ
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: above ground tissues
characteristics.characteristic: tissue-type
characteristics.text: ISUM2
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pAD-GAL4
characteristics.characteristic: vector