characteristics.text: SAMN00150489
characteristics.characteristic: External Id
characteristics.text: LIBEST_019889
characteristics.characteristic: External Id
characteristics.text: Rubber Research Institute of Malaysia
characteristics.characteristic: INSDC center name
characteristics.text: 2006-06-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:30.520Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RRIM600
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from latex of field-grown rubber trees. Following preparation of latex mRNA, subsequent steps of cDNA synthesis and library construction were performed using the ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack II Gold Cloning Kit (Stratagene). Latex cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once from the packaging reaction. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.
characteristics.characteristic: description
characteristics.text: Hevea brasiliensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3981
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Latex vessel
characteristics.characteristic: tissue-type
characteristics.text: YLX600
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR
characteristics.characteristic: vector