characteristics.text: SAMN00152425
characteristics.characteristic: External Id
characteristics.text: LIBEST_020478
characteristics.characteristic: External Id
characteristics.text: Centre for Cellular and Molecular Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:49.837Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: selectn-12 devel. Central Coffee Res. Inst., Chikamagalur,India
characteristics.characteristic: cultivar
characteristics.text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit.The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry.
characteristics.characteristic: description
characteristics.text: Coffea arabica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13443
characteristics.characteristic: organism
characteristics.text: selectn-12 devel. Central Coffee Res. Inst., Chikamagalur,India
characteristics.characteristic: strain
characteristics.text: moisture-stressed leaf tissue obtained from Department of Plant Physiology, University of Agricultural Sciences, Bangalore, India.
characteristics.characteristic: tissue-type
characteristics.text: Coffea arabica EST library
characteristics.characteristic: title
characteristics.text: pMOSBlue Blunt End vector
characteristics.characteristic: vector