characteristics.text: LIBEST_008880
characteristics.characteristic: External Id
characteristics.text: SAMN00163398
characteristics.characteristic: External Id
characteristics.text: University Paris12-Val de Marne
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:19.620Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Darmor
characteristics.characteristic: cultivar
characteristics.text: In Brassica napus and related species, lateral roots emerging under drought conditions remain short (1-2 mm) and hairless (Vartanian et al., 1994). A substractive cDNA library was constructed (CLONTECH PCR-Select cDNA Subtraction Kit), using mRNA of short roots to synthetize the tester cDNA, and cDNA obtained from fully developped droughted lateral roots as driver. Differential expression was confirmed by hybridization with cDNA probes. The method used yielded RsaI digested cDNA fragments of sizes ranging from 150 to 800 bp, which were fully sequenced. Each sequence has significant similarity matches within the Arabidopsis genome.
characteristics.characteristic: description
characteristics.text: Third leaf stage
characteristics.characteristic: dev-stage
characteristics.text: TOP10F'(Clontech)
characteristics.characteristic: lab-host
characteristics.text: lateral roots
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0020121
characteristics.characteristic: organ
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: T/A cloning
characteristics.characteristic: re_1
characteristics.text: Oleifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_145471
characteristics.characteristic: strain
characteristics.text: Short roots substractive library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT-Adv (Clontech)
characteristics.characteristic: vector