characteristics.text: LIBEST_000575
characteristics.characteristic: External Id
characteristics.text: SAMN00155178
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1996-12-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:14.727Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected cells histologically-determined to be fully malignant prostate cancer cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman.
characteristics.characteristic: description
characteristics.text: 45 years old
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004340
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not1
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: NCI_CGAP_Pr3
characteristics.characteristic: title
characteristics.text: Phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pAMP10
characteristics.characteristic: vector