characteristics.text: SAMN00164608
characteristics.characteristic: External Id
characteristics.text: LIBEST_022372
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:42.610Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Lottia gigantea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_225164
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: head
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000033
characteristics.characteristic: tissue-type
characteristics.text: CAWC Lottia gigantea from head (L)
characteristics.characteristic: title
characteristics.text: pMCL200_cDNA
characteristics.characteristic: vector