characteristics.text: SAMN00153285
characteristics.characteristic: External Id
characteristics.text: LIBEST_021291
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:14.467Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: FB3-425
characteristics.characteristic: cultivar
characteristics.text: Needles from 4 mature trees were harvested in the early fall (September). Equamolar amounts of mRNA from each treed was pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagenes pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Clones used in sequencing were from the primary library, with an amplification step.
characteristics.characteristic: description
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Picea sitchensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3332
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of the cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of the cDNA)
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Needles
characteristics.characteristic: tissue-type
characteristics.text: SS-NE-A-26
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector