characteristics.text: SAMN00166591
characteristics.characteristic: External Id
characteristics.text: LIBEST_016855
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2005-01-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:05.823Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (V(T)20CTCGAGAGAGAGAGAG). After ligation of PstI adapters and by XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
characteristics.characteristic: description
characteristics.text: Neurula (st. 14-18)
characteristics.characteristic: dev-stage
characteristics.text: TOP10 E. coli
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: PstI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: N6 (Nigerian 6th generation inbred)
characteristics.characteristic: strain
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: NIH_XGC_tropNeu5
characteristics.characteristic: title
characteristics.text: pCS22+
characteristics.characteristic: vector