characteristics.text: SAMN00164419
characteristics.characteristic: External Id
characteristics.text: LIBEST_009918
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:50.133Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B73
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007022
characteristics.characteristic: cultivar
characteristics.text: Tissues: Germinated seed and seedlings (1, 2, 8, 11 DAG), Mixed mature tissures (17, 21, 38, 69, 77 DAG), Kernels (3, 5, 10, 15, 20, 25, 30, DAP), Adventious roots (65 DAG), Tassel (3-39 cm, 53 and 56 DAG), Immature ear (0.2-3.0 cm, 53, 56, 59 DAG), Husk (73 DAG), Silk, unpollinated first ear, ear shank, etiolated seedlings, callus, Cycloheximide-treated callus, Anaerobic treated seedlings, NAA (a-Naphthalene acetic acid)-treated seedlings, Kinetin-treated seedlings, ACPC (1-aminocyclopropane-1-carboxylix acid)-treated seedlings, Brassinolide-treated seedlings, ABA (Abscisic acid)-treated seedlings, GA (Gibberellic acid)-treated seedlings, JA (Jasmonic acid)-treated seedlings. ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with NotI and size-selected. The resulting molecules were directionally cloned into the EcoRI and NotI sites of the pT7T3PAC vector. The library then went through one round of normalization to CoT value of 5 based on the methods of Marcelo Bento Soares (Genome Research 6: 791-806, 1996).
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: tissue-type
characteristics.text: ISUM5-RN
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3PAC
characteristics.characteristic: vector