characteristics.text: SAMN00158325
characteristics.characteristic: External Id
characteristics.text: LIBEST_003770
characteristics.characteristic: External Id
characteristics.text: Cardiovascular Institute, CAMS & PUMC
characteristics.characteristic: INSDC center name
characteristics.text: 2000-06-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:41.797Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The rat stroke model was developed by Wang Xianmei, Zhu Zhiming, Zhu Shanjun et al at 3rd Military Medical University Chongqing, China. The Suppression Subtraction Hybridization (SSH) was conducted at Fu Wai Hospital by Zhao Bin and Wang Xianmei in Beijing, China. Message RNA was extracted from pooled whole cerebrum from stroke and control rats respectively. SSH had been conducted following manufacture's manual using 5ug mRNA. The SSH library was constructed by Zhao Bin and Wang Xianmei using pMD18-T vector. Randomly picked clones were sequenced at ABI 377 using M13R primer(AGCGGATAACAATTTCACACAGG) by Wang Xianmei, Zhao Bin and Zhang Qian.
characteristics.characteristic: description
characteristics.text: 8-12 weeks old
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000025
characteristics.characteristic: dev-stage
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Wistar
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001342
characteristics.characteristic: strain
characteristics.text: Cerebrum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001893
characteristics.characteristic: tissue-type
characteristics.text: Plasmid Subtractive Library of Rat Cerebrum (stroke)
characteristics.characteristic: title
characteristics.text: Plasmid (T vector)
characteristics.characteristic: v_type
characteristics.text: pMD-T (TakaRa, D504 CA)
characteristics.characteristic: vector