characteristics.text: SAMN00153976
characteristics.characteristic: External Id
characteristics.text: LIBEST_012885
characteristics.characteristic: External Id
characteristics.text: 2003-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:09.180Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA made by oligo-dT priming and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5 kb size fraction. Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.
characteristics.characteristic: description
characteristics.text: DH10B (T1-phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: lung and heart
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SfiI (ggccattatggcc)
characteristics.characteristic: re_1
characteristics.text: SfiI (ggccgcctcggcc)
characteristics.characteristic: re_2
characteristics.text: NIH_MGC_178
characteristics.characteristic: title
characteristics.text: plasmid (chloramphenicol resistant, grown at 30C)
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector