characteristics.text: SAMN00153284
characteristics.characteristic: External Id
characteristics.text: LIBEST_021253
characteristics.characteristic: External Id
characteristics.text: Lund University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:13.473Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The insects were kept at 23 C, 70 percent humidity and either a 18:6 or a 20:4 h Light:Dark cycle. Total RNA was extracted from a pool of 15 female abdomens without ovipositors, hence pheromone glands were excluded. The abdomen RNA was combined in equal amount with total RNA extracted from a pool of 24 insect bodies without abdomens and wings. The insects were collected 0-3 days post emergence, at every fourth hour of the day in order to get a broad representation of different transcripts present in the insect. The cDNA library was constructed using the SMART cDNA Construction Kit (BD Biosciences) according to the manufacturers' protocol. Full length cDNAs were directionally ligated into (lambda) TriplEx2 arms after trimming by SfiI. The lambda library was converted into a plasmid library in a Cre+ b/w strain (E. coli BNN132). Plasmid clones were randomly collected and used as template for PCR amplification of inserts using the plasmid-specific primers P104 and P105, followed by DNA sequencing using the forward primer P104.
characteristics.characteristic: description
characteristics.text: Adults 0-3 days post emergence
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli BNN132
characteristics.characteristic: lab-host
characteristics.text: Agrotis segetum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_47767
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: As12, Biolog. Bundesanstalt f. Land u. Fort., Darmstadt, Ger.
characteristics.characteristic: strain
characteristics.text: Entire insect body apart from wings and ovipositor (i.e. pheromone gland was excluded)
characteristics.characteristic: tissue-type
characteristics.text: As12B - Agrotis segetum body library
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector