characteristics.text: SAMN00164678
characteristics.characteristic: External Id
characteristics.text: LIBEST_022440
characteristics.characteristic: External Id
characteristics.text: Institute of Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:44.990Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit taking the mRNA from the leaf tissue of salt (425 mM) treated plants as Tester and that from control (receiving no salt treatment) as Driver. The secondary PCR product was non-directionally cloned in a pGEM-T easy vector (Promega) using PCR Cloning Kit. The EST clones were sequenced using the SP6 Forward primer at TCGA (The Centre for Genomic Application), New Delhi.
characteristics.characteristic: description
characteristics.text: 2 months plant exposed to 425 mM Salt (NaCl) for 24 hours
characteristics.characteristic: dev-stage
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Suaeda maritima
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_126913
characteristics.characteristic: organism
characteristics.text: ECoRI
characteristics.characteristic: re_1
characteristics.text: ECoRI
characteristics.characteristic: re_2
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Suaeda maritima 425 mM salt stress (NaCl) SSH Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector