characteristics.text: LIBEST_022257
characteristics.characteristic: External Id
characteristics.text: SAMN00154609
characteristics.characteristic: External Id
characteristics.text: University of Florida
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:39.657Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: W22
characteristics.characteristic: cultivar
characteristics.text: The 3'anchored cDNA Sequences were generated using the 454-GS20 platform. cDNA was prepared from total RNA using a biotinylated dT-B adaptor to prime first strand synthesis. Double stranded cDNA was purified on streptavidin beads and digested with MspI. A custom A-adaptor that incorporated a 3 base multiplex key a CG overhang was ligated to the MspI ends. Following ligation, single stranded sequencing template was eluted and sequenced on the GS-20 instrument. Libraries were constructed from wild type and viviparous1 mutant ovaries and sequenced in a single multiplex reaction. Consensus sequences for anchored cDNA tags were obtained by CAP3 assembly. Additional details are included in the publication.
characteristics.characteristic: description
characteristics.text: prefertilization
characteristics.characteristic: dev-stage
characteristics.text: Ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organ
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: MspI
characteristics.characteristic: re_1
characteristics.text: W22 wild type and viviparous1 mutant
characteristics.characteristic: strain
characteristics.text: Maize ovary - Eveland 2007
characteristics.characteristic: title