characteristics.text: LIBEST_000344
characteristics.characteristic: External Id
characteristics.text: SAMN00154956
characteristics.characteristic: External Id
characteristics.text: Clontech Laboratories, Inc.
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:08.983Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Testis cDNAs were subtracted with a mixture of 10 cDNAs derived from 10 other human tissues using a method based on a newly developed suppression polymerase chain reaction (PCR) technique (to be published). This method amplifies only cDNA sequences that are unique to testis and arrests the PCR amplification of sequences that are common to the different tissue samples. After the subtraction, PCR products representing the subtracted cDNAs were inserted by TA cloning into the pCRII vector (Invitrogen).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Testis, Subtracted
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII
characteristics.characteristic: vector