characteristics.text: SAMN00002776
characteristics.characteristic: External Id
characteristics.text: CNRS
characteristics.characteristic: INSDC center name
characteristics.text: 2009-10-19T09:09:40.377Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:46:53.480Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS004335
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS004335
characteristics.characteristic: SRA accession
characteristics.text: RNA from three pooled preparations of MonoMac-6 cells, resuspended in 250 uL PBS and isolated with Trizol LS. A library of 19-33 nucleotide RNA was prepared according to the methods of Pfeffer S, Lagos-Quintana M, Tuschl T. Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.4. Cloning of Small RNA Molecules. RNA isolation, library preparation and 454 technology sequencing was performed in parallel with the library for exosome-like vesicles. The adaptors used for library preparation were as follows: 5' (454 A primer - identifying tag - 5' adaptor; GCCTCCCTCGCGCCATCAG-GTCA-GAATTCCTCACTAAA-cloned RNA) and 3'(cloned RNA - 3'adaptor - 454 B primer; TTTAACCGCGAATTC-CTGAGCGGGCTGGCAAGGC).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Generic sample from Homo sapiens
characteristics.characteristic: title