characteristics.text: LIBEST_019520
characteristics.characteristic: External Id
characteristics.text: SAMN00150157
characteristics.characteristic: External Id
characteristics.text: Israel Oceanographic and Limnological Research
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:20.690Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was prepared according to the subtractive- suppression hybridization method (SSH;Diatchenko, L., Lukyanov, S., Lau, Y.F.C. Siebert, P.D. 1999. Suppression subtractive hybridization: A versatile method for identifying differentially expressed genes. In: cDNA Preparation and Characterization, Meth. Enzymol. 303: 349-380) using two commercial kits (Clontech): SMART and PCR-select. cDNAs were cloned into P-GEM-T easy plasmid vectors transformed into the E. coli strain JM109. The hepatic cDNAs were cloned from fish exposed to a battery of pollutants.
characteristics.characteristic: description
characteristics.text: Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Lithognathus mormyrus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_50591
characteristics.characteristic: organism
characteristics.text: Striped sea bream hepatic library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: p-GEM-T easy
characteristics.characteristic: vector