characteristics.text: SAMN00154187
characteristics.characteristic: External Id
characteristics.text: LIBEST_017181
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2005-03-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:17.357Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA
characteristics.characteristic: cultivar
characteristics.text: Library enriched in transcripts present in early phase of loblolly pine embryo maturation. A subtractive library was made using the protocols of Clontech (Palo Alto,CA). RNA from loblolly pine embryos in which cotyledons had just emerged (Stage 9.1, see Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 [http://www.plantphysiol.org/cgi/content/full/127/4/1556]) was subtracted from RNA from pine embryos collected two weeks after cotyledon emergence (Stage 9.3).
characteristics.characteristic: description
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Zygotic Embryos
characteristics.characteristic: organ
characteristics.text: Pinus taeda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3352
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: SpeI
characteristics.characteristic: re_2
characteristics.text: Whole embryos excised from megagametophytes
characteristics.characteristic: tissue-type
characteristics.text: Subtracted pine embryo library, Lib_B
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T Easy
characteristics.characteristic: vector