characteristics.text: SAMN00153251
characteristics.characteristic: External Id
characteristics.text: LIBEST_021546
characteristics.characteristic: External Id
characteristics.text: Institute for Biochemistry, Faculty of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:20.720Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at day 4. We produced tester cDNA population of regenerated limbs which we hybridysed with driver cDNA population of intact tissue. By doing this we harvested down-regulated genes in regenerating tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen).All the obtained clones were sequenced.
characteristics.characteristic: description
characteristics.text: One Shot Competent Cells
characteristics.characteristic: lab-host
characteristics.text: limb
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002101
characteristics.characteristic: organ
characteristics.text: Ambystoma mexicanum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8296
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: strain
characteristics.text: Axolotl limb subtracted library, downregulated, day 4
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII
characteristics.characteristic: vector