characteristics.text: LIBEST_021734
characteristics.characteristic: External Id
characteristics.text: SAMN00153397
characteristics.characteristic: External Id
characteristics.text: University of Sao Paulo
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:25.923Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: embryonic cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002321
characteristics.characteristic: cell type
characteristics.text: BME26
characteristics.characteristic: cell-line
characteristics.text: directional cloned cDNA library. first strand synthesis with dT-attB2-Biotin primer. cDNAs > 350 bp selected by chromatography (Bio-Gel A50). double-stranded cDNAs ligated to attB1 adapter, size selected (> 350 bp) and cloned into pDONR-200 vector through recombination.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: dev-stage
characteristics.text: Rhipicephalus microplus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6941
characteristics.characteristic: organism
characteristics.text: embryonic cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002321
characteristics.characteristic: tissue-type
characteristics.text: Rhipicephalus (boophilus) microplus BME26 embryonic cell line library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDONR-222
characteristics.characteristic: vector