characteristics.text: LIBEST_022842
characteristics.characteristic: External Id
characteristics.text: SAMN00165386
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:57.007Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into vector pTriplEx2 (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. Library constructed in the laboratory of J. Stillman (San Francisco State University, San Francisco, CA) and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found in the Porcelain Crab Array Database; http://array.sfsu.edu.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Petrolisthes cinctipes
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_88211
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: pooled heart, nerve, whole crab
characteristics.characteristic: tissue-type
characteristics.text: CAYC Petrolisthes cinctipes heart, nerve, whole crab
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector