characteristics.text: LIBEST_021126
characteristics.characteristic: External Id
characteristics.text: SAMN00152877
characteristics.characteristic: External Id
characteristics.text: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:10.157Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sporulated oocysts were obtained from cecea of E.tenella infected chickens.sporulated oocysts subtracted cDNA library using cDNA from sporulated oocysts cDNA as testers and cDNA from unsporulated oocysts as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (Clontech). PCR products then had been directionally subcloned into the pGEM-T Easy vector (Promega) and transformed into JM109 competent cells (Promega).
characteristics.characteristic: description
characteristics.text: Sporulated oocysts
characteristics.characteristic: dev-stage
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Eimeria tenella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5802
characteristics.characteristic: organism
characteristics.text: CAAS21111601
characteristics.characteristic: strain
characteristics.text: Eimeria tenella sporulated oocysts subtracted cDNA library
characteristics.characteristic: title
characteristics.text: pGEM-T Easy
characteristics.characteristic: vector