characteristics.text: LIBEST_022161
characteristics.characteristic: External Id
characteristics.text: SAMN00165566
characteristics.characteristic: External Id
characteristics.text: Malaysian Palm Oil Board
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.597Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 1.38 X 10^9 pfu/ml.
characteristics.characteristic: description
characteristics.text: Non-Embryogenic
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue_MRF'
characteristics.characteristic: lab-host
characteristics.text: Elaeis guineensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_51953
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Callus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0005052
characteristics.characteristic: tissue-type
characteristics.text: CA (Oil Palm Non-Embryogenic Callus)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript_SK_plus
characteristics.characteristic: vector