characteristics.text: LIBEST_018251
characteristics.characteristic: External Id
characteristics.text: SAMN00165026
characteristics.characteristic: External Id
characteristics.text: University of Wisconsin
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-03T23:44:12.283Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:41:35.267Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nipponbare
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007587
characteristics.characteristic: cultivar
characteristics.text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and NOACRR1_2R (5' GAT GAA CCC TGA GTT CCW CT 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band II was used for cloning.
characteristics.characteristic: description
characteristics.text: four weeks old plant
characteristics.characteristic: dev-stage
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Oryza sativa Japonica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: organism
characteristics.text: LIB_ID:44
characteristics.characteristic: title