characteristics.text: SAMN00165360
characteristics.characteristic: External Id
characteristics.text: LIBEST_022815
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:55.667Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Kanlow
characteristics.characteristic: cultivar
characteristics.text: Library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. Library is non-amplified. Tissue was provided by G. Sarath (USDA ARS, Albany, CA), library was constructed in the laboratory of P. Twigg (University of Nebraska, Kearney) and sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: DH5alpha FT
characteristics.characteristic: lab-host
characteristics.text: whole seedlings
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Panicum virgatum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_38727
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: CBYZ Panicum virgatum Kanlow 4 day old seedlings
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector