characteristics.text: LIBEST_008662
characteristics.characteristic: External Id
characteristics.text: SAMN00163188
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:13.237Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E.tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on sephacryl S500. The cDNA were ligated to EcoRI/XhoI prepared lambda ZapII (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cell (Stratagene). Clones were selected by negative hybridization against a pool of overrepresented ESTs (N>=10,from 1682 previous reads). Insert sizes range from 1.2-2.9Kb. The library may contain a small percentage of host or bacterial contaminents.
characteristics.characteristic: description
characteristics.text: Sporozoite stage
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Eimeria tenella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5802
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: LS18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006640
characteristics.characteristic: strain
characteristics.text: Eimeria tenella S5-2 cDNA Neg Selected
characteristics.characteristic: title
characteristics.text: phagemid (Amp. resistant)
characteristics.characteristic: v_type
characteristics.text: Bluescript SK-
characteristics.characteristic: vector