characteristics.text: LIBEST_021278
characteristics.characteristic: External Id
characteristics.text: SAMN00153011
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:14Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A17
characteristics.characteristic: cultivar
characteristics.text: Medicago truncatula A17 plants, were grown in pots containing 75% Metro Mix 350 soil and 25% sand. Glands were dislodged from tissues by vigorously vortexing tissues in an isotonic buffer. Glands were harvested by sieving out larger tissues and by differential centrifugation. RNA for library construction was isolated from pure gland preparations using the Qiagen RNA Easy kit. The Creator SMART cDNA Library Construction Kit from Clontech was used for library construction.
characteristics.characteristic: description
characteristics.text: Glands were isolated from young pre-flowering stems and leaves.
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: Sfi I A (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: Sfi I B (GGCCGCCTCGGCC)
characteristics.characteristic: re_2
characteristics.text: Isolated glandular trichomes
characteristics.characteristic: tissue-type
characteristics.text: Medicago truncatula A17 glandular trichome
characteristics.characteristic: title
characteristics.text: pDNR_lib (Clontech)
characteristics.characteristic: vector