characteristics.text: SAMN00166668
characteristics.characteristic: External Id
characteristics.text: LIBEST_023709
characteristics.characteristic: External Id
characteristics.text: University of California, San Francisco
characteristics.characteristic: INSDC center name
characteristics.text: 2008-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:23.987Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was ligated to the Gene Racer oligo (Invitrogen), reverse-transcribed, and amplified using SHANK3-specific reverse primers with PfuUltra high-fidelity DNA polymerase (Stratagene) under the following 3-step 'touch-down' cycling parameters: (1) 5 cycles of 94oC for 30 sec, 72oC for 1 min, (2) 5 cycles of 94oC for 30 sec, 70oC for 1 min, (3) 30 cycles of 94oC for 30 sec, 62oC for 30 sec, and 72oC for 1 min, followed by 72oC for 10 min. The amplification products were gel purified, cloned into the pCR4-TOPO vector (Invitrogen), and inserts were sequenced.
characteristics.characteristic: description
characteristics.text: post-natal day 56
characteristics.characteristic: dev-stage
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: whole brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: tissue-type
characteristics.text: Mouse post-natal day 56 brain 5'-RACE
characteristics.characteristic: title