characteristics.text: LIBEST_020109
characteristics.characteristic: External Id
characteristics.text: SAMN00152631
characteristics.characteristic: External Id
characteristics.text: Indian Institute of Technology, Kharagpur
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:38.507Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: JRC 321
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from fibre forming stage of jute stem by hot borate method and purified by isopycnic CsCl density gradient ultracentrifugation. Poly (A+) mRNA was isolated using oligo (dT) cellulose. The cDNA was synthesized from the Poly (A+) mRNA using oligo (dT) 12-18 primer with the help of cDNA synthesis Time saver TM kit (Amersham) following the instruction of the manufacturer. The double stranded cDNA synthesized was size fractionated using Sephacryl S-300. The double stranded cDNA was then ligated to the EcoRI/NotI adapter, followed by kinasing and purification by Sephadex G50 column. The purified cDNA was ligated to EcoRI digested and Shrimp alkaline phosphatase treated pUC18 plasmid vector. Finally, the ligated product was transformed in E.coli DH10B competent cells. The library was hybridized with double stranded cDNA probe generated from poly(A)+mRNA of non-fibre forming stage of jute stem and unhybridized clones, which are expected to be involved in bast fibre development along with other developmental processes, were selected. The selected clones were then subjected to single pass sequencing. The resultant sequences were edited to remove the vector sequence. The clones were putatively identified by searching against plant database in TIGR using tblastx.
characteristics.characteristic: description
characteristics.text: 45 days after sowing
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: organ
characteristics.text: Corchorus capsularis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_210143
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Bast fiber and associated tissues
characteristics.characteristic: tissue-type
characteristics.text: Jute Plasmid library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC18
characteristics.characteristic: vector