characteristics.text: LIBEST_023258
characteristics.characteristic: External Id
characteristics.text: SAMN00165831
characteristics.characteristic: External Id
characteristics.text: MPI for Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:09.207Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from 5 male specimens of weight ranging between 400 and 500 g. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Dicentrarchus labrax
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13489
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: tissue-type
characteristics.text: cDN22
characteristics.characteristic: title