characteristics.text: LIBEST_022977
characteristics.characteristic: External Id
characteristics.text: SAMN00165522
characteristics.characteristic: External Id
characteristics.text: The International Institute of Tropical Agriculture
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:00.750Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Dan Ila, Tvu11986, Vu7778 and 12008D
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from pools of leaf and stem meristem tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed cowpea lines Dan Ila, Tvu11986, Vu7778 and 12008D. A single total RNA sample was derived by combining RNAs from leaf and stem meristem on a 1:1 quantity basis. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.
characteristics.characteristic: description
characteristics.text: mature, pre flowering
characteristics.characteristic: dev-stage
characteristics.text: Vigna unguiculata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3917
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: leaf and shoot meristem
characteristics.characteristic: tissue-type
characteristics.text: MOP
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector