characteristics.text: LIBEST_012359
characteristics.characteristic: External Id
characteristics.text: SAMN00153924
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles
characteristics.characteristic: INSDC center name
characteristics.text: 2002-12-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:56.123Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hammond's Dwarf Scarlet
characteristics.characteristic: cultivar
characteristics.text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from suspensor mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The suspensor cDNA fragments were directionally ligated into the SfiI restriction site of the lambda TriplEx2 vector (Clontech), and the recombinant cDNAs were transformed into E. coli XL1-Blue cells (Clontech). Suspensor cDNA plasmids used for directional sequencing were obtained by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 cells (Clontech).
characteristics.characteristic: description
characteristics.text: 6-days post-pollination
characteristics.characteristic: dev-stage
characteristics.text: Suspensor Region of Globular-Stage Embryos
characteristics.characteristic: organ
characteristics.text: Phaseolus coccineus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3886
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: Scarlet Runner Bean Suspensor Region TriplEx2
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: TriplEx2
characteristics.characteristic: vector