characteristics.text: LIBEST_022752
characteristics.characteristic: External Id
characteristics.text: SAMN00165300
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-02-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:53.963Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: Stressed by acid (pH 6.0). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.
characteristics.characteristic: description
characteristics.text: Adults plus neonates up to third instar
characteristics.characteristic: dev-stage
characteristics.text: TOP10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007796
characteristics.characteristic: lab-host
characteristics.text: Daphnia pulex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6669
characteristics.characteristic: organism
characteristics.text: Sfi I A
characteristics.characteristic: re_1
characteristics.text: Sfi I B
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: The Chosen One (arenata)
characteristics.characteristic: strain
characteristics.text: whole animal
characteristics.characteristic: tissue-type
characteristics.text: CBNP_Daphnia_pulex_Chosen_One_Library_6
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector