characteristics.text: SAMN00149829
characteristics.characteristic: External Id
characteristics.text: LIBEST_019154
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2006-02-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:11.347Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suncrisp
characteristics.characteristic: cultivar
characteristics.text: Total RNA was extracted from freeze dried fruit tissue, using the method described by Wang and Vodkin(Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III(Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly(dT)sequence with a Xho-I restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells.
characteristics.characteristic: description
characteristics.text: 35 days after pollination
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009010
characteristics.characteristic: dev-stage
characteristics.text: DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Malus domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3750
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Fruit tissue
characteristics.characteristic: tissue-type
characteristics.text: Apple_EST_Mdfrs
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK (+)
characteristics.characteristic: vector