characteristics.text: SAMN00156857
characteristics.characteristic: External Id
characteristics.text: LIBEST_002294
characteristics.characteristic: External Id
characteristics.text: NIDDK, National Institutes of Health
characteristics.characteristic: INSDC center name
characteristics.text: 1999-08-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:03.703Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated ~1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored.
characteristics.characteristic: description
characteristics.text: 1-5 day adult
characteristics.characteristic: dev-stage
characteristics.text: SOLR (Stratagene)
characteristics.characteristic: lab-host
characteristics.text: testis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000473
characteristics.characteristic: organ
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: y[*] w[67c1]/Y
characteristics.characteristic: strain
characteristics.text: Drosophila melanogaster adult testis library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScript SK (Stratagene)
characteristics.characteristic: vector