characteristics.text: LIBEST_008845
characteristics.characteristic: External Id
characteristics.text: SAMN00163364
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-04-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:18.733Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-Oligo(dT) PCR-based library. Parastrongyloides trichosuri cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis.
characteristics.characteristic: description
characteristics.text: Infective larvae
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Parastrongyloides trichosuri
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_131310
characteristics.characteristic: organism
characteristics.text: Parastrongyloides trichosuri IL SL1 TOPO v2
characteristics.characteristic: title
characteristics.text: plasmid (amp. or kan. resistant)
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector