characteristics.text: LIBEST_009747
characteristics.characteristic: External Id
characteristics.text: SAMN00164253
characteristics.characteristic: External Id
characteristics.text: Michigan State University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:45.783Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: USH20
characteristics.characteristic: cultivar
characteristics.text: cDNAs were derived from reverse transcription of mRNA samples from immature inflorescence. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from root, leaf and 4-day old germinated seedlings. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBK-CMV vector (Stratagene).
characteristics.characteristic: description
characteristics.text: XLOLR
characteristics.characteristic: lab-host
characteristics.text: inflorescence
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009049
characteristics.characteristic: organ
characteristics.text: Beta vulgaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_161934
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: whole inflorescence
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007047
characteristics.characteristic: tissue-type
characteristics.text: Sugar Beet inflorescence cDNA library (subtracted)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBK-CMV
characteristics.characteristic: vector