characteristics.text: LIBEST_007264
characteristics.characteristic: External Id
characteristics.text: SAMN00161794
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles, School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-12-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:31.170Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: bFGF Cultured Neurospheres
characteristics.characteristic: cell type
characteristics.text: Cells were harvested from P0 mouse cortex and cultured for 4 weeks in the presence of bFGF to grow neurospheres. Half of the cells were then placed under differentiating conditions for 24 hours. RNA was extracted from both groups using acid phenol extraction, PolyA(+) RNA was purified using oligo dt paramagnetic beads, and cDNA was synthesized. Amplicons were created from Dpn II digested cDNA and 2 rounds of RDA subtraction were performed using amplicons from the differentiated cells as the driver. Resultant library, enriched in clones from the undifferentiated neural progenitors, was then shotgun cloned into BamH1 digested Bluescript SK II+ and transformed into E. coli.
characteristics.characteristic: description
characteristics.text: P0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002948
characteristics.characteristic: dev-stage
characteristics.text: Hbl electromax DH10B (GibcoBrl)
characteristics.characteristic: lab-host
characteristics.text: Brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: CD1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005180
characteristics.characteristic: strain
characteristics.text: Cerebral Cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000956
characteristics.characteristic: tissue-type
characteristics.text: neural progenitor enriched library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript KS II+
characteristics.characteristic: vector