characteristics.text: SAMN00154305
characteristics.characteristic: External Id
characteristics.text: LIBEST_019473
characteristics.characteristic: External Id
characteristics.text: 1. College of animal science, Zhejiang University, 2. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry.
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:19.520Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Shin Ichinose
characteristics.characteristic: cultivar
characteristics.text: Total RNA was extracted from growing buds of mulberry cultivar 'Shin Ichinose' and its mutant 'Fengwei Ichinose' by the method of Trizol (Invitrogen) in summer, 'Fengwei Ichinose' is a leaf mutant of 'Shin Ichinose' induced by gamma ray of Co60. Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). mRNA was reverse transcribed into double stranded cDNA using the SMART cDNA library construction kit (CLONTECH). Template preparation, PCR procedure and polyacrylamide gel electrophoresis of cDNA-AFLP was performed according the method described by Bachem (Plant Molecular Biology Reporter 16:157-173, 1998). The gels were performed using the silver staining. The different cDNA fragments between the wild type 'Shin Ichinose' and its mutant 'Fengwei Ichinose' were directionally cloned into the pUC19 T-vector. The ligated cDNA fragments were transformed into E. coli DH5alpha host cells and sequenced.
characteristics.characteristic: description
characteristics.text: bud and young leaf
characteristics.characteristic: organ
characteristics.text: Morus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3498
characteristics.characteristic: organism
characteristics.text: Growing bud library in mulberry leaf mutant by cDNA-AFLP
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC19
characteristics.characteristic: vector