characteristics.text: SAMN00150295
characteristics.characteristic: External Id
characteristics.text: LIBEST_019682
characteristics.characteristic: External Id
characteristics.text: Instituto Tecnologico de Celaya Departamento de Ingenieria Bioquimica
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:24.870Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 2 ul PCR fragments were ligated with 1 ul pCR4 -TOPO cloning vector according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). 2 ul ligation reaction solutions were transformed into 50 ul E. coli chemically competent cells strain TOP 10. The transformation culture was plated on Petri dishes containing LB/kamamycin, and the screened colony for insert fragment. Selected colonies containing recombinant plasmid were inoculated separately into 5 ml LB/ampicillin/kanamycin solution and it was shaken at 37 oC overnight. Then 500 ul each culture were added into 2 ml cryogenic vial (Corning, Acton, MA, USA) containing 500 ul 100 % glycerol and kept at -80 oC.
characteristics.characteristic: description
characteristics.text: 3 days in water
characteristics.characteristic: dev-stage
characteristics.text: Allium sativum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4682
characteristics.characteristic: organism
characteristics.text: Blanco perla
characteristics.characteristic: strain
characteristics.text: Clove
characteristics.characteristic: tissue-type
characteristics.text: Allium Reverse Subtractive Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR 4 TOPO TA
characteristics.characteristic: vector