characteristics.text: SAMN00165948
characteristics.characteristic: External Id
characteristics.text: LIBEST_023378
characteristics.characteristic: External Id
characteristics.text: Mount Desert Island Biological Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:13.540Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: melanoma derived
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0008229
characteristics.characteristic: cell type
characteristics.text: XM cell line
characteristics.characteristic: cell-line
characteristics.text: The XM cell line was derived from from skin and fin of two tumor-bearing Xiphophorus BC1 hybrids (Xiphophorus maculatus x Xiphophorus helleri x Xiphophorus helleri; Xiphophorus Genetic Stock Center, San Marcos, TX). The Hyb BBC1 helleri x X. maculatus was strain H005BC1, Pedigree 8921, date of birth 9/16/03. The X. maculatus used were Strain 163A, virgin females from flock date of birth 12/19/03. Cells were grown in culture as described by David Barnes, Lori Dowell, David Forest, Angela Parton, Peter Pavicevic, and Steven Kazianis. 2006. Characterization of XM, a Novel Xiphophorus Melanoma-Derived Cell Line. Zebrafish 3(3): 371-381. Using TRIzol reagent, total RNA was isolated from 0.5 x10e8 cells. The primary cDNA library was constructed from mRNA and directionally cloned into pCMV-SPORT6.1. The host bacterium was DH10B T1 (T1 and T5 phage resistant). Standard cDNA construction and quality control were performed by Invitrogen. The average insert size was 1.7 kb and the unamplified titer was 1.27 x 10e6 cfu/ml.
characteristics.characteristic: description
characteristics.text: two adults
characteristics.characteristic: dev-stage
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Xiphophorus maculatus x Xiphophorus hellerii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_96538
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRV
characteristics.characteristic: re_2
characteristics.text: melanoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000756
characteristics.characteristic: tissue-type
characteristics.text: XMC_Ea
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSport6_1 with ccdB verA
characteristics.characteristic: vector