characteristics.text: LIBEST_005585
characteristics.characteristic: External Id
characteristics.text: SAMN00160123
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:37.070Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Blanco
characteristics.characteristic: cultivar
characteristics.text: Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
characteristics.characteristic: description
characteristics.text: Seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH12S
characteristics.characteristic: lab-host
characteristics.text: Secale cereale
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4550
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Root tip
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000025
characteristics.characteristic: tissue-type
characteristics.text: Secale cereale aluminum-stressed root tip cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector