characteristics.text: LIBEST_020025
characteristics.characteristic: External Id
characteristics.text: SAMN00150618
characteristics.characteristic: External Id
characteristics.text: CNRS/EPHE/UPVD
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:34.783Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: whole body
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001489
characteristics.characteristic: cell type
characteristics.text: mRNA was harvested from snail susceptible and resistant towards Echinostoma caproni. Combined cDNAs from the two snail strains were subtracted using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR4(r)-TOPO(r) (Invitrogen, Carlsbad, CA) and sequenced using the forward plasmid-specific primer T7.
characteristics.characteristic: description
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Bg.CB
characteristics.characteristic: strain
characteristics.text: BgCBWB
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR4(r)-TOPO(r) (Invitrogen)
characteristics.characteristic: vector