characteristics.text: LIBEST_023577
characteristics.characteristic: External Id
characteristics.text: SAMN00166470
characteristics.characteristic: External Id
characteristics.text: Rubber Research Institute of India
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:19.153Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PR 261
characteristics.characteristic: cultivar
characteristics.text: Total RNA was prepared from leaf tissues of rubber tree subjected to cold stress and also from the normal plant belonging to the same clones. Messenger RNA was purified from total RNA using PolyATract mRNA Isolation System (Promega). cDNA synthesis followed by suppression subtractive hybridization (SSH) was carried out using the PCR Select cDNA Subtraction Kit (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned randomly into the vector pTZ57R/T (MBI Fermentas). Colony PCR using vector directed primers was used to identify recombinant plasmids containing inserts. Recombinant plasmids were subjected to single-pass sequencing reactions using M13 forward primer.
characteristics.characteristic: description
characteristics.text: Hevea brasiliensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3981
characteristics.characteristic: organism
characteristics.text: Mature leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: GA_HbSCDNA Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pTZ57R/T (MBI Fermentas)
characteristics.characteristic: vector