characteristics.text: LIBEST_001416
characteristics.characteristic: External Id
characteristics.text: SAMN00155995
characteristics.characteristic: External Id
characteristics.text: Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1998-09-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:39.953Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B lymphocytes were isolated from excised tonsils, activated with PMA(5ng/ml). Total RNA and mRNA were isolated from activated B lymphocytes. A cDNA library was constructed with SuperScript Plasmid System for cDNA synthesis and Plasmid Cloning (GIBCO BRL). Then single-stranded DNA(ssDNA) of the library was prepared with M13 helper phage and hybridized with cDNA of resting lymphocytes. Double-stranded DNA(dsDNA) was removed with hydroxyapatite and the remaining ssDNA was repaired to dsDNA and transformed E. coli JM109. Clones with long inserts were further studied.
characteristics.characteristic: description
characteristics.text: E. coli JM109
characteristics.characteristic: lab-host
characteristics.text: tonsil
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002372
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Subtracted cDNA library of activated B lymphocyte
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pSport1
characteristics.characteristic: vector