characteristics.text: SAMN00152717
characteristics.characteristic: External Id
characteristics.text: LIBEST_020961
characteristics.characteristic: External Id
characteristics.text: UC Davis, Department of Pomology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.250Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Paradox clone 'PX1'
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated using the hot borate method from roots of germinated somatic embryos on DKW media in culture plates. Total RNA was purified (Qiagen RNA/DNA Mini Kit) and used to construct a cDNA library (CLONTECH SMART cDNA Library kit). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated form the resultant bacterial population. Plasmid DNA from individual transformed colonies was sequenced using plasmid primer sequences (Washington University Genome Sequence Center, St. Louis, MO).
characteristics.characteristic: description
characteristics.text: In vitro grown roots from germinated somatic embryos
characteristics.characteristic: dev-stage
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Juglans hindsii x Juglans regia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_432290
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: WRO-01: Walnut root
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Lambda TriplEX2
characteristics.characteristic: vector