characteristics.text: LIBEST_021881
characteristics.characteristic: External Id
characteristics.text: SAMN00154522
characteristics.characteristic: External Id
characteristics.text: The University of Melbourne
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:29.913Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA sequences were produced from lactation stage specific cDNA cloning of mRNA obtained from day 23 pregnant, day 130, day 260 of lactation or 4 days unsucked gland. First strand cDNA was prepared from total RNA (1ug) or mRNA (500ng) using the SMART II oligonucleotide and MMLV reverse transcriptase as recommended by the manufacturer (Promega) except for library 15 which used a thermostable reverse transcriptase (Thermoscript, Invitrogen). PCR was used to make second strand cDNA according the the SMART system instructions (Promega). The first strand cDNA was then ligated with pGEMTeasy (Promega) using the recommended ligation reaction conditions and reagents. In some cases, the second strand cDNA was treated further prior to ligation when size selection of cDNA molecules was achieved by two methods. For libraries 5-10, the cDNA was fractionated by agarose gel electrophoresis and fragments of different sizes were purified using silicon beads (Qiaex beads, Qiagen). For libraries 16-19, small cDNA fragments were removed by sephadex column chromatography using a CHROMA SPIN-1000 column (Promega). Electroporation was used to transform E.coli DH5a with ligated material and recombinant bacteria were selected for ampicillin resistance.
characteristics.characteristic: description
characteristics.text: unsucked gland at 4 days of lactation
characteristics.characteristic: dev-stage
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: organ
characteristics.text: Notamacropus eugenii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9315
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: tissue-type
characteristics.text: library_18: tammar wallaby mammary tissues from unsucked gland at 4 days of lactation
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEMTeasy
characteristics.characteristic: vector