characteristics.text: LIBEST_019677
characteristics.characteristic: External Id
characteristics.text: SAMN00154327
characteristics.characteristic: External Id
characteristics.text: Carnegie Institution of Washington
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:24.763Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Subtractive hybridization was performed with the PCR-Select Subtraction Kit (Clontech), according to the manufacturerÕs protocol. Tester tissue consisted of whole-body 15 day post-hatch (dph) larvae that had been treated with methimazol (0.1M) from 12-15 dph. Driver tissue was treated with methimazol and triiodothyronine (50nM).
characteristics.characteristic: description
characteristics.text: 15 days post-hatch
characteristics.characteristic: dev-stage
characteristics.text: Whole-body larvae
characteristics.characteristic: organ
characteristics.text: Paralichthys lethostigma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_141886
characteristics.characteristic: organism
characteristics.text: Rsa I
characteristics.characteristic: re_1
characteristics.text: Rsa I
characteristics.characteristic: re_2
characteristics.text: Larval flounder thyroid hormone (72 hrs) down-regulated mRNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector