characteristics.text: LIBEST_016633
characteristics.characteristic: External Id
characteristics.text: SAMN00154147
characteristics.characteristic: External Id
characteristics.text: Chinese Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2004-10-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:58.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: skeletal muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001134
characteristics.characteristic: cell type
characteristics.text: A porcine skeletal muscle forward subtracted cDNA library was constructed by suppression subtractive hybridisation (SSH). We firstly set up two RNA pools with muscular RNA come from double-muscled pig(DM) and non-double-muscled pig(NDM) individually, and then constructed the subtracted cDNA library by suppression subtractive hybridization (SSH).
characteristics.characteristic: description
characteristics.text: 2.5 months piglet
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: musculus longissimus dorsi
characteristics.characteristic: organ
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: Rsa I
characteristics.characteristic: re_1
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: purebred
characteristics.characteristic: strain
characteristics.text: musculus longissimus dorsi
characteristics.characteristic: tissue-type
characteristics.text: Forward subtracted cDNA library from skeletal muscle of double-muscled large white pig
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMD-T18
characteristics.characteristic: vector