characteristics.text: LIBEST_019368
characteristics.characteristic: External Id
characteristics.text: SAMN00150028
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:16.620Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was constructed by Wayne Hunter and Phat Dang (U. S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA) using the Stratagene cDNA library kit. Total RNA was extracted using Qiagen RNA extraction kits, DNAse Treated (Promega), single pass Poly(A) isolated (Ambion). cDNAs were cloned into phage, packaged, and titered with primary library consisting greater than 1 X 10^7 pfu/mL. Phage libraries were amplified, and mass-excised for library sequencing. The mass-excised libraries (pBlueScript SK) phagemids were reinfected into MRF' XLI Blue cells.
characteristics.characteristic: description
characteristics.text: Pre-pupal
characteristics.characteristic: dev-stage
characteristics.text: Nasonia giraulti
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7426
characteristics.characteristic: organism
characteristics.text: RV2 (Wolbachia infected)
characteristics.characteristic: strain
characteristics.text: Nasonia giraulti (wasp) pre-pupal EST library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector