characteristics.text: LIBEST_019686
characteristics.characteristic: External Id
characteristics.text: SAMN00150301
characteristics.characteristic: External Id
characteristics.text: Department of Genetics and Biochemistry, Clemson University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:24.953Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sweet basil seeds were grown under natural light conditions in the greenhouse. Greenhouse cooling/heating set points were 27/25oC. At the 3rd leaf stage, 0.5 mM MeJA in 2% ethanol was sprayed onto the basil plants. The untreated (control) basil plants were only sprayed with 2% ethanol. Then the MeJA treated and control plants were zipped with a vinyl pack for 40 min. Each of one gram of leaves from the untreated (control) and the MeJA treated sweet basil was randomly sampled at 0, 1, 3, 6, 9, 12, 24, 48 hours. The leaves were immediately stored at -80 oC prior to analysis. Total RNA was extracted respectively from the pooled leaves of the MeJA treated and the control using Trizol method (Invitrogen) according to the manufacturer's recommendations. SSH was performed using a PCR Select(tm) cDNA Subtraction Kit (Clontech) according to the manufacturer's instructions. The subtracted cDNA library was cloned directly into the T/A cloning vector pCR2.1 (Invitrogen). Differential screening was performed using colony array (PCR-Select Differential Screening Kit, Clontech).
characteristics.characteristic: description
characteristics.text: the 3rd leaf stage (30-40d old seedling)
characteristics.characteristic: dev-stage
characteristics.text: E. coli Top 10F'
characteristics.characteristic: lab-host
characteristics.text: Ocimum basilicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39350
characteristics.characteristic: organism
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Differential Gene Expression upon Methyl Jasmonate (MeJA) Treatment
characteristics.characteristic: title
characteristics.text: pCR2.1
characteristics.characteristic: vector