characteristics.text: SAMN00155649
characteristics.characteristic: External Id
characteristics.text: LIBEST_001062
characteristics.characteristic: External Id
characteristics.text: Institute of Forest Genetics, USDA Forest Service
characteristics.characteristic: INSDC center name
characteristics.text: 1998-06-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:30.930Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: variety = menziesii; mRNA was isolated from new growth needle tissue and cloned uni-directionally into Uni-ZAP XR with the ZAP-cDNA Synthesis Kit (Stratagene). The phagemid Bluescript SK (-) was excised from the phage vector using the in vivo excision protocol provided by Stratagene. The nucleotide sequence of the 3' end was obtained by automated sequencing at the Recombinant DNA/Protein Resource Facility, Oklahoma State University, Oklahoma, USA.
characteristics.characteristic: description
characteristics.text: spring growth
characteristics.characteristic: dev-stage
characteristics.text: Pseudotsuga menziesii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3357
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: needle tissue
characteristics.characteristic: tissue-type
characteristics.text: Douglas-fir (Pm_1000) cDNA library
characteristics.characteristic: title
characteristics.text: phage
characteristics.characteristic: v_type
characteristics.text: Uni-Zap XR Lambda (Stratagene)
characteristics.characteristic: vector