characteristics.text: SAMN00165707
characteristics.characteristic: External Id
characteristics.text: LIBEST_023130
characteristics.characteristic: External Id
characteristics.text: UMR 7100 - IFR 85 - ESBS -ULP
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:05.397Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: cultivar
characteristics.text: Total-RNA extraction was performed with the RNaqueous-4PCR kit (Ambion, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The second strand of the cDNA was synthesized with the FIREPol Taq DNA Polymerase I (Solis Biodyne, Estonia). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).
characteristics.characteristic: description
characteristics.text: E.coli TOP10 F'
characteristics.characteristic: lab-host
characteristics.text: Flower
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009046
characteristics.characteristic: organ
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: HincII
characteristics.characteristic: re_1
characteristics.text: HincII
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Humulus lupulus Male flower cDNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC19
characteristics.characteristic: vector