characteristics.text: LIBEST_020419
characteristics.characteristic: External Id
characteristics.text: SAMN00154364
characteristics.characteristic: External Id
characteristics.text: ViaLactia Biosciences (NZ) Ltd.
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:48.333Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly-A mRNA was prepared from the mammary gland of a single pregnant lactating Friesian cow by oligo-dT cellulose affinity chromatography. The primary library was was constructed from poly-A-enriched RNA using the ZAP-cDNA Gigapack III Gold Kit (StrataGene) as intructed by the manufacturer, and normalized by an adaption of method 4 (Bonaldo et al., 1996) using in vitro-produced single-stranded circles as tracer.
characteristics.characteristic: description
characteristics.text: Lactating
characteristics.ontologyTerms: http://opendata.inra.fr/ATOL/ATOL_0001682
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: lab-host
characteristics.text: Mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: organ
characteristics.text: Bos taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Friesian
characteristics.characteristic: strain
characteristics.text: Mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: tissue-type
characteristics.text: FLM
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(+/-)
characteristics.characteristic: vector