characteristics.text: LIBEST_003800
characteristics.characteristic: External Id
characteristics.text: SAMN00158353
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-03-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:42.583Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: tcgacccacgcgtccg and 3' adaptor: gactagttctagatcgcgagcggccgcccttttttttttttttt. Average insert size 1.3kb. Primary library, non-amplified. Library constructed by N. Pollet, PhD. REF: Gawantka, V., Pollet, N., Delius, H., Vingron, M., Pfister, R., Nitsch, R., Blumenstock, C., and Niehrs, C. (1998) Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning. Mech. Dev. 77:95-141. Note: This is a Xenopus Gene Collection (XGC) library.
characteristics.characteristic: description
characteristics.text: stage 13
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007106
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue, F+
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: Xenla 13
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript KS+ (Stratagene)
characteristics.characteristic: vector