characteristics.text: LIBEST_021909
characteristics.characteristic: External Id
characteristics.text: SAMN00154536
characteristics.characteristic: External Id
characteristics.text: Universite Laval
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:30.657Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Tissue: Dormant shoot tips dissected from buds. Bud scales and developing needles were removed, leaving a piece of tissue approximately 3 mm in length. Sample comprises shoot apex, leaf primordia as well as subtending primary stem tissues. Endodormant trees: after completion of second growth cycle, trees were transferred progressively from 20 deg C days/20 deg C nights to 20 deg C days/10 deg C nights for 3 weeks, 4 deg C days/4 deg C nights for 2 weeks, and then 2 deg C days/2 deg C nights until sampling. Trees were at 2 deg C for 5.5 weeks prior to sampling. Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA
characteristics.characteristic: description
characteristics.text: Endodormant trees
characteristics.characteristic: dev-stage
characteristics.text: Xl1-Blue
characteristics.characteristic: lab-host
characteristics.text: Terminal bud
characteristics.characteristic: organ
characteristics.text: Picea glauca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3330
characteristics.characteristic: organism
characteristics.text: Sfi1-A
characteristics.characteristic: re_1
characteristics.text: Sfi1-B
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite (Monoecious)
characteristics.characteristic: sex
characteristics.text: Five individuals from an association population, representing families 370, 372, 663, 685, and 986
characteristics.characteristic: strain
characteristics.text: Dormant shoot tips dissected from buds
characteristics.characteristic: tissue-type
characteristics.text: GQ037 - Shoot tip - Dormant
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAL17.3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector