characteristics.text: LIBEST_019289
characteristics.characteristic: External Id
characteristics.text: SAMN00166263
characteristics.characteristic: External Id
characteristics.text: Harbin Institute of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:14.777Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: First-strand cDNA was synthesized with an XhoI-oligo(dT) adapter primer. After second-strand synthesis and ligation of EcoRI adapters, cDNA was digested with XhoI, generating cDNA flanked by EcoRI sites at 5' ends and XhoI sites at the 3' ends. Purified cDNA were size-fractionated by electrophoresis and ligated into the vector of pBluescript II predigested with EcoRI and XhoI.
characteristics.characteristic: description
characteristics.text: Incubation for 36h
characteristics.characteristic: dev-stage
characteristics.text: Trichoderma lixii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1491472
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: T88
characteristics.characteristic: strain
characteristics.text: mycelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001436
characteristics.characteristic: tissue-type
characteristics.text: Trichoderma harzianum cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector