characteristics.text: LIBEST_009638
characteristics.characteristic: External Id
characteristics.text: SAMN00164149
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.987Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: M. truncatula seeds were acid treated and placed on wet filter papers in petri dishes. Seeds were harvested at 0, 1, 2 and 3 days after acid treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
characteristics.characteristic: description
characteristics.text: 0, 1, 2 and 3 days after acid treatment.
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: germinating seeds
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007057
characteristics.characteristic: tissue-type
characteristics.text: Germinating Seed
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007057
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Lambda Zap
characteristics.characteristic: vector