characteristics.text: LIBEST_004051
characteristics.characteristic: External Id
characteristics.text: SAMN00158604
characteristics.characteristic: External Id
characteristics.text: Albert Einstein College of Medicine of Yeshiva University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:49.430Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suppression subtractive hybridization was performed between 13 dpc fetal rat liver as a tester and adult rat liver as a driver using PCR-Select cDNA Subtraction Kit (Clontech). Tester and driver cDNA were digested with Rsa I; adaptors were ligated to the tester. Two rounds of subtraction hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into pGEM T-Easy (Promega).
characteristics.characteristic: description
characteristics.text: embryonic day 13 post-ferilization
characteristics.characteristic: dev-stage
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Sprague-Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: tissue-type
characteristics.text: Rat pGEM T-Easy Subtracted Library
characteristics.characteristic: title