characteristics.text: LIBEST_009765
characteristics.characteristic: External Id
characteristics.text: SAMN00164271
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:46.187Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Library was constructed by Debopam Chakrabarti. Total RNA samples were isolated from mixed stage saponin(0.1%)-lysed P. falciparum 3D7 infected erythrocytes by the acidic guanidinium-phenol chloroform method. The poly A+ RNA was isolated by the polyAT-Tract mRNA isolation system (Promega, WI) using streptavidin Magnesphere particles. Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA (5mg) into EcoRI and XhoI sites of l ZapII vector using the Zap cDNA synthesis kit (Stragene, CA). The average size of the cDNA inserts in the library was between 1.0 and 1.5kb. Clones were mass excised using the ExAssist helper phage (Stratagene), the phagemids were precitptated with PEG 8000 and extracted with phenol/chloroform. Phagemid DNA was electroporated into DH10B cells.
characteristics.characteristic: description
characteristics.text: DH10B (GeneHog, Invitrogen, Inc.)
characteristics.characteristic: lab-host
characteristics.text: Plasmodium falciparum 3D7
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_36329
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Plasmodium falciparum 3D7 asexual cDNA
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK plus
characteristics.characteristic: vector