characteristics.text: LIBEST_022676
characteristics.characteristic: External Id
characteristics.text: SAMN00165192
characteristics.characteristic: External Id
characteristics.text: UMR INRA/Universite Henri Poincare 1136
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:51.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mycelium of Lacaria bicolor S238N was grown 21 days on cellophane-covered agar containing modified Pachlewski medium (pH 5.5). This consisted of (mM) 12.5 (NH4)2tartrate, 110 D-glucose, 14 maltose, 7 KH2PO4, 2 MgSO4; microelements (gl-1) 6.00 iron, 0.27 molybdenum, 8.45 boron, 5.00 manganese, 0.625 copper, 2.27 zinc, Kanieltra 6Fe, and thiamine-HCl, 100 mg l-1. Colonies were then transferred on complete Pachlewski liquid medium for two days acclimatising period and then N, P or C starved during a time course experiment of 6 days. Control mycelium was grown in complete Pachlewski liquid medium. Fruiting bodies of L. bicolor S238N were collected below Douglas fir seedlings grown in a nursery and inoculated using L. bicolor S238N. Tissues were immediately frozen in liquid nitrogen and RNA extractions were carried out using the RNeasy Plant Mini Kit (Qiagen). Total RNA from different tissues was pooled (18% derived from fruiting bodies) and mRNA was purified using Oligotex mRNA spin columns (Qiagen). cDNA was synthesized using the CLONTECH SMART cDNA library synthesis Kit(Ozyme). First-strand cDNA synthesis was performed as described in the provided protocol using 150 ng mRNA. Double-stranded cDNA was prepared from 2ul of the first strand reaction by PCR (25 cycles) with the provided primers in a 100ul reaction and cDNA was purified using a QIAquick PCR purification column (Qiagen). Adapter ligation, nebulization and DNA sequencing was performed by COGENICS (France) according to their protocol and using a 70x75mm region of a genome sequencer GS-FLX run (Roche Diagnostics).
characteristics.characteristic: description
characteristics.text: free-living mycelium and fruiting bodies
characteristics.characteristic: organ
characteristics.text: Laccaria bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29883
characteristics.characteristic: organism
characteristics.text: S238N
characteristics.characteristic: strain
characteristics.text: Free-living mycelium and fruiting bodies from Laccaria bicolor S238N
characteristics.characteristic: title