characteristics.text: SAMN00150886
characteristics.characteristic: External Id
characteristics.text: LIBEST_020324
characteristics.characteristic: External Id
characteristics.text: United States Department of Agriculture, Agricultural Research Service
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:45.880Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Directional cDNA libraries were constructed in the Lambda Uni-ZAP(r) XR vector using 5ug of total RNA according to the manufacturers' instructions (Stratagene, LaJolla, CA). Whiteflies (B.tabaci, B biotype) were reared on non-infected cotton plants. The plants were kept in insect-proof cages. Insects were collected randomly from the colony and placed at -80oC. Total RNA was isolated from pools of approximately 200 insects using the RNeasy Midi kit (QIAGEN). Contaminating DNA was discarded using RNAase-free DNAse I. Double stranded cDNA was synthesized using the cDNA Synthesis Kit (Stratagene) with primers containing each EcoRI and XhoI sites. Blunt ends were created, ligated to EcoRI adaptors, phosphorylated and digested with EcoRI. The cDNAs were size-fractionated using Drip Columns to obtain molecules longer than 300 bp. The cDNA was ligated to the Zap Express vector and used to transform E. coli cells in the presence of kanamycin. The phagemid pBK-CMV was packaged using the Gigapack III Gold packaging (Stratagene). Phages were used to inoculate XL1-Blue MRF cells. A library was generated with a titer of 5.2_1010 plaque-forming units per ml. The library was mass excised using Ex-Assist(r) helper phage (Stratagene) and bacterial clones containing excised pBluescript SK+ phagemids were recovered by random colony selection.
characteristics.characteristic: description
characteristics.text: Cotton
characteristics.characteristic: lab-host
characteristics.text: Bemisia tabaci
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7038
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: B biotype
characteristics.characteristic: strain
characteristics.text: Whitefly Bemisia tabaci (non-viruliferous) cDNA library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK+
characteristics.characteristic: vector