characteristics.text: SAMN00161351
characteristics.characteristic: External Id
characteristics.text: LIBEST_006816
characteristics.characteristic: External Id
characteristics.text: Medical College of Shantou University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:17.720Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SHEE(Immortalized esophageal epithelial cell line) and SHEEC(Esophageal cancer cell line)
characteristics.characteristic: cell-line
characteristics.text: The library is prepared by using PCR_SelectTM cDNA Subtraction Kit (Clontech). Briefly, the first cDNA is synthesized from polyA+RNA from the two types of cells being compared, SHEE(Immortalized esophageal epithelial cell line) and SHEEC(Esophageal cancer cell line), the second two subtractive hybridizations are performed, the third the entire population of molecules is subjected to PCR to amplify the desired differentially expressed sequences, then the cDNAs are directly inserted into a T/A cloning vector(pT_Adv vector, clontech), the last differentially expressed RNAs are identified by sequence and Northern hybridization analysis.
characteristics.characteristic: description
characteristics.text: TOP10F' (Clontech)
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Human esophageal cancer differentially expressed library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT_Adv(Clontech)
characteristics.characteristic: vector