characteristics.text: SAMN00152407
characteristics.characteristic: External Id
characteristics.text: LIBEST_020617
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2006-11-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:54.977Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Guar (Cyamopsis tetragonoloba) plants, cultivar HES 1401, were grown in 2 gallon pots containing 75% Metro Mix 350 soil and 25% sand at a temperature of 26oC/22oC (day/night). Plants were fertilized at time of watering using a commercial Peters Professional 20-10-20 (N-P-K) fertilizer mix. Seeds were harvested 15, 20, 25, 30, 35, and 40 days after flowering (DAF). cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. A 'late' seed library was generated from seeds 30, 35, and 40 DAF. The cDNA was directionally ligated into the Uni-Zap XR vector and packaged using Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF'. Excised plasmids were plated using SOLR cells.
characteristics.characteristic: description
characteristics.text: late developmental stage (30-40 days after flowering, DAF)
characteristics.characteristic: dev-stage
characteristics.text: Cyamopsis tetragonoloba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3832
characteristics.characteristic: organism
characteristics.text: EcoRI (GAATTC)
characteristics.characteristic: re_1
characteristics.text: XhoI (CTCGAG)
characteristics.characteristic: re_2
characteristics.text: seed
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009010
characteristics.characteristic: tissue-type
characteristics.text: Cyamopsis tetragonoloba (L.) Taub seeds at late developmental stage
characteristics.characteristic: title
characteristics.text: pBluescript sk
characteristics.characteristic: vector