characteristics.text: LIBEST_021218
characteristics.characteristic: External Id
characteristics.text: SAMN00152960
characteristics.characteristic: External Id
characteristics.text: Pennsylvania State University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-06-04T21:03:09.123Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Library for RNA-ligase mediated 5'-RACE. polyA+ RNA was first reacted with T4 RNA ligase and an RNA adapter without CIP pre-treatment, and thus was enriched in uncapped mRNAs. The pool was then reverse transcribed with an oligodT primer to make 1st strand cDNA. Various gene-specific primers were then used to find truncated cDNAs corresponding to the 3' fragments left by microRNA-mediated mRNA cleavage.
characteristics.characteristic: description
characteristics.text: Physcomitrium patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: Protonemata and young leafy gametophores
characteristics.characteristic: tissue-type
characteristics.text: 17 day-old wild-type P. patens
characteristics.characteristic: title