characteristics.text: LIBEST_019507
characteristics.characteristic: External Id
characteristics.text: SAMN00166820
characteristics.characteristic: External Id
characteristics.text: Okazaki National Research Institutes
characteristics.characteristic: INSDC center name
characteristics.text: 2012-04-18T14:40:38.253Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:20.313Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DH5-alpha; Plasmodium ookinetes were generated by in vitro culture of gametocytes (sexual stage parasites collected from parasite infected rats) and purified with density gradient centrifugation using NycoPrep (1.077 A; AXIS-SHIELD PoCAS). mRNAs were extracted using micro-mRNA extraction kit (Pharmacia). Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA into SalI and NotI sites of pSPORT 1 vector using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit (Life Technologies, Inc.)
characteristics.characteristic: description
characteristics.text: ookinete
characteristics.characteristic: dev-stage
characteristics.text: Plasmodium berghei ANKA
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5823
characteristics.characteristic: organism
characteristics.text: ANKA
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5823
characteristics.characteristic: strain
characteristics.text: Plasmodium berghei ookinete
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector