characteristics.text: LIBEST_014192
characteristics.characteristic: External Id
characteristics.text: SAMN00166078
characteristics.characteristic: External Id
characteristics.text: National Institute for Agricultural Research (INRA)
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:48.157Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A cDNA library was made with mRNA isolated from tension wood tissues corresponding to the differentiating xylem collected with a scalpel after debarking the stem. The sampling was done on 3 different tilted trees grown in the nursery. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)
characteristics.characteristic: description
characteristics.text: 3-years-old poplar trees grown in the nursery
characteristics.characteristic: dev-stage
characteristics.text: Populus tremula x Populus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80863
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: clone 717-1-B4
characteristics.characteristic: strain
characteristics.text: Young differentiating xylem harvested on the tension wood side of tilted trees
characteristics.characteristic: tissue-type
characteristics.text: Poplar cDNA library from young tension xylem
characteristics.characteristic: title