characteristics.text: LIBEST_009863
characteristics.characteristic: External Id
characteristics.text: SAMN00164366
characteristics.characteristic: External Id
characteristics.text: Institute of Tropical Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.783Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: hemocytes
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000387
characteristics.characteristic: cell type
characteristics.text: Total RNA was isolated from hemocytes of uninfected and infected Biomphalaria glabrata (13-16-R1) snails and first strand cDNA was synthesized using anchored oligo-(dT)-primers (H-T11-A/C/G; GenHunter, Nashville, TN). 33P-labeled second strand synthesis was carried out using one of eight 13-mer arbitrary primers (GenHunter, Nashville, TN) in a PCR based amplification reaction. The labeled PCR products were separated using 6% polyacrylamide/8M urea gels and the differentially expressed fragments were identified by autoradiography. Bands of interest were sliced out from the gel and the DNA was extracted. This DNA was reamplified using the identical primer combination and SSCP analysis was performed. Differential expression of this fragment was not confirmed. However the PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen, Groningen, NL) and then subjected to sequencing. Insert was amplified using T3 and T7 vector specific primers.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: 13-16-R1
characteristics.characteristic: strain
characteristics.text: hemocytes of infected (6-48h) Biomphalaria glabrata 13-16-R1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR4-TOPO
characteristics.characteristic: vector