characteristics.text: SAMN00154149
characteristics.characteristic: External Id
characteristics.text: LIBEST_016686
characteristics.characteristic: External Id
characteristics.text: Children's Memorial Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2004-11-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:00.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: UI-R-DY1 is a normalized cDNA library containing the following tissue(s): Rat Cartilage from Femur and Tibia. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTAATGGACG. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa.
characteristics.characteristic: description
characteristics.text: ADULT
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Femur and Tibia
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Sprague-Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: Cartilage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0007844
characteristics.characteristic: tissue-type
characteristics.text: UI-R-DY1
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector