characteristics.text: SAMN00164274
characteristics.characteristic: External Id
characteristics.text: LIBEST_009768
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:46.307Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly A+ RNA was isolatd from the testes of 31 male adult zebrafish (4-5 month old). cDNAs were made using oligo-dT primers and inserted into lambda ZAP II vector (Stratagene) by Dr. Z. Gong, in vivo mass-excised to pBluescript SK- following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Zhiyuan Gong for further information on this library (National University of Singapore, Department of Biological Sciences, Lower Kent Ridge Road, Singapore 119260).
characteristics.characteristic: description
characteristics.text: 4-5 month
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000035
characteristics.characteristic: dev-stage
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: testis (pooled)
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Gong zebrafish testis
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector