characteristics.text: LIBEST_014258
characteristics.characteristic: External Id
characteristics.text: SAMN00154025
characteristics.characteristic: External Id
characteristics.text: University of Wales Aberystwyth,Institute of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:49.837Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Three-week-old Brachypodium distachyon plants (accession ABR5) were infected with pathogen (isolate GUY11 of Magnaporthe grisea, which is a Oryzae sativa isolate). RNA was prepared from infected above ground parts harvested from 3-week-old plants 18,48,72,96 and 144 hr after inoculation. Note: accession ABR5 exhibits a resistance response to isolate GUY11. The library was made from poly-A RNA in the cloning vector lambda ZAP II. The cDNA library was differentially screened with 32P labelled first stand cDNA derived from control 0.2% gelatin treated (144 hr) plants and pooled RNAs used to construct the library. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.
characteristics.characteristic: description
characteristics.text: 18,48,72,96 and 144 hr after infection
characteristics.characteristic: dev-stage
characteristics.text: Above ground parts of M.grisea infected plants
characteristics.characteristic: organ
characteristics.text: Brachypodium distachyon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_15368
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Pathogen induced ABR5/GUY11
characteristics.characteristic: title
characteristics.text: pBluescript II from lambda Zap II
characteristics.characteristic: vector