characteristics.text: SAMN00149790
characteristics.characteristic: External Id
characteristics.text: LIBEST_012275
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:04.080Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:12.510Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cabernet Sauvignon
characteristics.characteristic: cultivar
characteristics.text: CA41LU is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were asymptomatic and were verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs.cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.
characteristics.characteristic: description
characteristics.text: Late season sample
characteristics.characteristic: dev-stage
characteristics.text: DH5alpha
characteristics.characteristic: lab-host
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Cabernet Sauvignon Leaf - CA41LU
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR
characteristics.characteristic: vector