characteristics.text: LIBEST_014666
characteristics.characteristic: External Id
characteristics.text: SAMN00166082
characteristics.characteristic: External Id
characteristics.text: *RZPD Ressourcenzentrum fuer Genomforschung GmbH, **Institute of Plant Genetics and Crop Plant Research Gatersleben
characteristics.characteristic: INSDC center name
characteristics.text: 2004-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:01.310Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Barke
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0004518
characteristics.characteristic: cultivar
characteristics.text: 0-10 DAF (days after flowering), cDNA synthesis using pBluescript II XR cDNA-library construction kit (Stratagen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter (Invitrogen). The main library of 21500 clones was rearrayed into the sublibrary BES 1824 containing 4100 putative expression clones. Note: Due to a cloning artefact caused by the kit, in most cases the SalI site is NOT present, as well as the SalI Adapter used for cloning. To excise the insert, restriction sites upstream SalI should be used (e.g. BamHI). Average insert size is 1 kb. Library generation and sequencing was granted in context of GABI; data are also accessible at https://gabi.rzpd.de
characteristics.characteristic: description
characteristics.text: 0-10 DAF (days after flowering)
characteristics.characteristic: dev-stage
characteristics.text: E. coli, SCS-1/pSE111
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: embryosac
characteristics.characteristic: tissue-type
characteristics.text: BES1824
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005725
characteristics.characteristic: title
characteristics.text: pQE30NST (AF074376)
characteristics.characteristic: vector