characteristics.text: LIBEST_001853
characteristics.characteristic: External Id
characteristics.text: SAMN00156418
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 1999-06-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:52.717Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: genotype A17
characteristics.characteristic: cultivar
characteristics.text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
characteristics.characteristic: description
characteristics.text: Immediately prior to inoculation with Sinorhizobium meliloti (0 hour)
characteristics.characteristic: dev-stage
characteristics.text: E.coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Seedling roots
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: tissue-type
characteristics.text: KV0
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK -
characteristics.characteristic: vector