characteristics.text: LIBEST_019833
characteristics.characteristic: External Id
characteristics.text: SAMN00150448
characteristics.characteristic: External Id
characteristics.text: Forestry and Forest Products Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2006-06-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:28.410Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: To obtain mixed-stage nematodes, the nematodes were cultured on fungi (Botrytis cinerea grown on autoclaved barley) for 1 week at 25C and then extracted for 3h at 25C using the Baermann funnel technique. Separated nematodes were washed with 0.1M NaCl. Poly(A)+ RNA was extracted using an RNeasy kit (Qiagen) and subsequently an oligotex-dT30 mRNA purification Kit (Takara). The cDNA library was constructed using the SuperScript Plasmid system (Invitrogen)(vector: pSPORT1; RE_1: SalI; RE_2: NotI). The plasmid construct was transformed into E. coli DH5a by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).
characteristics.characteristic: description
characteristics.text: mixed stage
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Bursaphelenchus xylophilus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6326
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Ka4
characteristics.characteristic: strain
characteristics.text: Bursaphelenchus xylophilus mixed-stage library
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector