characteristics.text: SAMN00152480
characteristics.characteristic: External Id
characteristics.text: LIBEST_020689
characteristics.characteristic: External Id
characteristics.text: NIDCD/NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:57.240Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly A+ RNAs were extracted from circumvallate taste papillae (tester) and non-taste lingual epithelium (driver). Following a pre-amplification step using the Smart PCR cDNA Synthesis kit (K1052-1, BD Biosciences), circumvallate papillae cDNA was subtracted against non-taste lingual epithelium cDNA using the PCR-Select cDNA subtraction kit (K1804-1, BD Biosciences) according to manufacturer's protocols, which are available in detail at http://www.clontech.com/. Subtracted cDNAs were non-directionally cloned into the T/A cloning site of pCRII (Invitrogen). The sequencing of individual clones, and EST analysis were performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: tongue
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001723
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: C57Bl6
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: taste buds
characteristics.characteristic: tissue-type
characteristics.text: mouse taste receptor cell, subtracted, normalized
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII
characteristics.characteristic: vector