characteristics.text: SAMN00003006
characteristics.characteristic: External Id
characteristics.text: University of Washington Genome Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2009-05-29T11:30:09.480Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:50:14.360Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS003000
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS003000
characteristics.characteristic: SRA accession
characteristics.text: For the males we 1) grow dpy-28(y1);him-8(e1489) strain at 15 deg until majority of animals are gravid. 2) We bleach the worms to collect embryos, and then place the embryos in S-basal buffer an d incubate at 25 deg over night to hatch the L1s. 3) We then put the hatched L1s on a seeded NGM plate at 25 deg for 30 hours. At 30 hours, the resulting worms are in the L4 stage, and all are males because the hermpahrodites die due to a failure to initiate dosage compensation, as confirmed by examination under the dissecting microscope. 4) At this time, the worms are harvested, placed into Trizol, and then subjected to three rounds of freeze/thaw, before being submitted for RNA isolation. Quality values are still in solexa/illumina format and have not been converted to phred values.
characteristics.characteristic: description
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Generic sample from Caenorhabditis elegans
characteristics.characteristic: title