characteristics.text: SAMN00161762
characteristics.characteristic: External Id
characteristics.text: LIBEST_007230
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2000-12-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:30Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Chinese Spring
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007588
characteristics.characteristic: cultivar
characteristics.text: Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
characteristics.characteristic: description
characteristics.text: Adult plant
characteristics.characteristic: dev-stage
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Crown
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000043
characteristics.characteristic: tissue-type
characteristics.text: Wheat salt-stressed crown cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR, excised phagemid
characteristics.characteristic: vector