characteristics.text: LIBEST_019835
characteristics.characteristic: External Id
characteristics.text: SAMN00150450
characteristics.characteristic: External Id
characteristics.text: Forestry and Forest Products Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2006-06-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:28.457Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B. xylophilus dauer larvae (dispersal 4th stage larvae) were produced using the artificial pupal chamber method in a pine log as described in (Aikawa and Togashi, 1998). Dauer larvae which migrated into the vector beetles were separated by Baermann funnel extraction (KDi). Dauer larvae which remained in the logs were collected from the wood surface by hand using a platinum wire picker after the logs had been cut into pieces (KDw). Separated nematodes were washed with 0.1M NaCl and 30% sucrose. mRNA was extracted using Micro-FastTrack (Invitrogen). cDNA library was constructed using the SMART PCR cDNA amplification method (Clontech). SMART-amplified cDNAs were directionally ligated into the pDNR-lib vector. Each plasmid construct was transformed into Escherichia coli DH5alpha by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).
characteristics.characteristic: description
characteristics.text: Dauer (dispersal 4th stage larvae)
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Bursaphelenchus xylophilus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6326
characteristics.characteristic: organism
characteristics.text: Ka4
characteristics.characteristic: strain
characteristics.text: Bursaphelenchus xylophilus dauer stage cDNA library
characteristics.characteristic: title
characteristics.text: pDNR-lib
characteristics.characteristic: vector