characteristics.text: LIBEST_018807
characteristics.characteristic: External Id
characteristics.text: SAMN00149823
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:04.600Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:15.360Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total and poly A+ RNA was isolated from the indicated stickleback tissue, and a cDNA library was constructed in the Express 1 plasmid vector by Open Biosystems. First strand cDNA synthesis was primed with an 54 bp linker primer containing an oligodT sequence preceeded by a synthetic NotI site (first strand primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'). Following second strand synthesis, cDNAs were made blunt at the end corresponding to the original 5 prime end of mRNA, and cloned directionally into the NotI and EcoRV sites of Express 1. Note that the EcoRV site is typically destroyed in the blunt end cloning, leaving a junction of the form xxxATC(where is ATC is the second half of the EcoRV site, and xxx is derived from the cDNA sequence). A map of the Express 1 vector is available at: http://www.openbiosystems.com/cdna_library_construction_faq.php#8 The primary library was transformed and amplified in DH10B (T1 phage resistant) bacteria.
characteristics.characteristic: description
characteristics.text: 21 day old larvae collected at Swarup Stage 30 (J. Embryol. Exp. Morphol 6: 373-383.1958)
characteristics.characteristic: dev-stage
characteristics.text: DH10B (T1 phage resistant)
characteristics.characteristic: lab-host
characteristics.text: Gasterosteus aculeatus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_69293
characteristics.characteristic: organism
characteristics.text: mixed male and female
characteristics.characteristic: sex
characteristics.text: Bitrufjordur marine sticklebacks, Iceland
characteristics.characteristic: strain
characteristics.text: whole larva
characteristics.characteristic: tissue-type
characteristics.text: CLJ2
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Express 1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003788
characteristics.characteristic: vector