characteristics.text: LIBEST_000880
characteristics.characteristic: External Id
characteristics.text: SAMN00155478
characteristics.characteristic: External Id
characteristics.text: University of Berne
characteristics.characteristic: INSDC center name
characteristics.text: 1997-06-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:24.203Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: total RNA isolated from liquid cultures grown to late log phase in Middlebrook 7H9/ADC in an atmosphere of 5% oxygen, 5% CO2 and 90% Nitrogen. Random fragments were amplified from total RNA by rPCR with the primer UPN6 (5'-GCCGGAGCTCTGCAGAATTCNNNNNN-3') and cloned into EcoR1 site of pET24a (+). Clones from M.tb ribosomal RNA were detected by hybridization and removed.
characteristics.characteristic: description
characteristics.text: late log phase
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli strain Novablue (Novagen)
characteristics.characteristic: lab-host
characteristics.text: Mycobacterium tuberculosis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1773
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: EcoR1
characteristics.characteristic: re_2
characteristics.text: PI, ATCC 51910
characteristics.characteristic: strain
characteristics.text: Mtb FOX (5% oxygen) expression library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pET24a (+) (Novagen)
characteristics.characteristic: vector