characteristics.text: SAMN00153915
characteristics.characteristic: External Id
characteristics.text: LIBEST_012100
characteristics.characteristic: External Id
characteristics.text: Stanford University
characteristics.characteristic: INSDC center name
characteristics.text: 2002-11-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:48.803Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: OH43
characteristics.characteristic: cultivar
characteristics.text: Schmidt lab dissected immature ears between 0.5 cm - 2.0 cm. Sharon Stanfield prepared the cDNA library in HybriZAP. Positive selection by probing with the pooled full-length cDNA of the following MADS box genes: ZAGL8B, ZAGL9B, ZAGL17, SI, ZAG1, ZAG2, ZAP1A, ZMM2, and ZPIA. Negative selection by probing with pooled 3'-end fragments of the same DNA. The final library is derived from a total of 210 selected plaques that hybridized with the full-length cDNA probes, but not with the 3'-end cDNA probes.
characteristics.characteristic: description
characteristics.text: 0.5 cm - 2.0 cm
characteristics.characteristic: dev-stage
characteristics.text: XLOLR
characteristics.characteristic: lab-host
characteristics.text: immature ear
characteristics.characteristic: organ
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: ear
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001690
characteristics.characteristic: tissue-type
characteristics.text: 3528 - Positive selection of MADS-box genes from ear library 946
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pAD-GAL4
characteristics.characteristic: vector