characteristics.text: LIBEST_020750
characteristics.characteristic: External Id
characteristics.text: SAMN00152529
characteristics.characteristic: External Id
characteristics.text: USDA/ARS
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:58.847Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The E. acervulina cDNA was cloned into the Uni-ZAP XR Vector (Stratagene) incorporating the EcoRI and XhoI cloning sites at the 5' and 3' end of the insert respectively. A mass in vivo excision was carried out producing a pBluescript SK (-) plasmid library (Stratagene). The effective library titer was approximately 8x106 cfu/ml. The excised library was propagated in the SOLR E. coli strain, and stocks were frozen in LB medium containing 15% glycerol. The library was constructed by Amplicon Express (Pullman, WA).
characteristics.characteristic: description
characteristics.text: second generation merozoite and schizont
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Eimeria acervulina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5801
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: 12
characteristics.characteristic: strain
characteristics.text: E. acervulina cDNA from second generation merozoites and schizonts
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector