characteristics.text: SAMN00152843
characteristics.characteristic: External Id
characteristics.text: LIBEST_021088
characteristics.characteristic: External Id
characteristics.text: University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:08.497Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from pooled brain tissues using modified Trizol method (Invitrogen), then mRNA was extracted and purified from total RNA using Oligotex mRNA kit (Qiagen). cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (Sfi I) vector using SMARTTM cDNA library construction kit according to manufacturer's instructions (Clontech). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Lymnaea stagnalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6523
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end)
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Whole ganglia ring was isolated from animals before and after a CNS regeneration procedure
characteristics.characteristic: tissue-type
characteristics.text: LSG10-01
characteristics.characteristic: title
characteristics.text: pBluescript II SK (SfiI)
characteristics.characteristic: vector