characteristics.text: LIBEST_022811
characteristics.characteristic: External Id
characteristics.text: SAMN00165356
characteristics.characteristic: External Id
characteristics.text: Div. of Crop Protection, Central Research Institute for Jute and Allied Fibres
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:55.567Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Patharkuchi
characteristics.characteristic: cultivar
characteristics.text: Apical leaf (3rd from top) of tomato plants (15 days old) were mechanically inoculated with the partially clarified sap obtained from leaves of rice (Oryza sativa cv. Khitish) plants infected with RNMV (Rice necrosis mosaic virus). When enlargement in leaf size as compared to control was noticed in inoculated plants, apical leaves were collected and frozen. Total RNA was isolated from the frozen leaves and subtractive hybridization was carried out. Final PCR product containing only the upregulated genes in response to inoculation was cloned in pJET 1.2 vector and sequenced.
characteristics.characteristic: description
characteristics.text: Newly emerging leaves
characteristics.characteristic: dev-stage
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: Leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Library of tomato plant treated with RNMV infected rice leaf sap
characteristics.characteristic: title