characteristics.text: LIBEST_002715
characteristics.characteristic: External Id
characteristics.text: SAMN00157274
characteristics.characteristic: External Id
characteristics.text: Max-Planck-Institute for Chemical Ecology
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:14.673Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hydroponically grown plants of the inbred line DI92 of Nicotiana attenuata were exposed to herbivory by first-instar larvae of the tobacco hornworm (Manduca sexta, Lepidoptera, Sphingidae) for 24 hrs. Total cellular RNAs were extracted from rosette leaves and served as templates for mRNA differential display RT-PCR. The cDNAs of insect-responsive genes were cloned into the pCR2.1-TOPO vector (Invitrogen) and subjected to sequencing.
characteristics.characteristic: description
characteristics.text: Vegetative stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007134
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli TOPO 10F' (Invitrogen)
characteristics.characteristic: lab-host
characteristics.text: Nicotiana attenuata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_49451
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Inbred Line Utah DI92
characteristics.characteristic: strain
characteristics.text: Leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: cDNAs of Nicotiana attenuata insect-responsive genes isolated by mRNA differential-display RT-PCR
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1-TOPO (Invitrogen)
characteristics.characteristic: vector