characteristics.text: SAMN00154935
characteristics.characteristic: External Id
characteristics.text: LIBEST_000323
characteristics.characteristic: External Id
characteristics.text: The Natural History Museum
characteristics.characteristic: INSDC center name
characteristics.text: 1995-09-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:08.563Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total mRNA was isolated from adult worms using the acid guanidine thiocyanate method (Chomczynski and Sacchi, 1987). Poly (A)+ RNA was purified by affinity chromatography on oligo dT column (Aviv and Leder, 1972). The cDNA expression library was constructed using the Lambda Zap cDNA cloning system (Stratagene-[Uni-Zap XR Gigapack II cloning kit). The library was amplified (end titre=0.5 x 10E8 pfu/ml) and its efficiency tested for using known S.mansoni probes (chorion and tropomyosin genes). The size range of inserts for the library was 0.5Kb - 2.5 Kb, the cDNA being directionally cloned between the EcoRI and the XhoI sites of the Bluescript phagemid. Bluescript phagemid was excised by mass in vivo excision of the library and inserted into E.coli XL1 Blue. The plasmid was purified using the Qiagen Qiawell 8 system and sequenced with Taq dye-terminator chemistry (SK,T3 and T7 primers) for ABI 373A. The library was prepared by Hanan Abdel Hamed, Ain Shams Univ.,Cairo and provided by Mohamed Saber, Theodore Bilharz Research Institute, Cairo.
characteristics.characteristic: description
characteristics.text: Golden Hamster
characteristics.characteristic: lab-host
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Egyptian
characteristics.characteristic: strain
characteristics.text: SmAW
characteristics.characteristic: title
characteristics.text: Bluescript
characteristics.characteristic: vector