characteristics.text: LIBEST_022808
characteristics.characteristic: External Id
characteristics.text: SAMN00165353
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:55.483Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into Invitrogen vector pDONR222 (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with a biotin-attB2-Oligo(dT) primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp., Carlsbad, CA). The second strand was synthesized and an attB1 adaptor ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCCC-3'). cDNA was size selected using column chromatography and then inserted into the vector using site specific recombination (flanking attB sites on cDNA). RNA prepared at University of California, San Diego, library constructed at Kyoto University (Kyoto, Japan), and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: gastrula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000109
characteristics.characteristic: dev-stage
characteristics.text: Branchiostoma floridae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7739
characteristics.characteristic: organism
characteristics.text: attB1
characteristics.characteristic: re_1
characteristics.text: attB2
characteristics.characteristic: re_2
characteristics.text: whole animal
characteristics.characteristic: tissue-type
characteristics.text: Amphioxus Branchiostoma floridae unpublished cDNA library CAXF, gastrula whole animal
characteristics.characteristic: title
characteristics.text: pDONR222
characteristics.characteristic: vector