characteristics.text: SAMN00016863
characteristics.characteristic: External Id
characteristics.text: Biology Centre, Institute of Parasitology, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia
characteristics.characteristic: INSDC center name
characteristics.text: 2011-03-28T18:10:46.167Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:57:21.610Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS084838
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS084838
characteristics.characteristic: SRA accession
characteristics.text: Chromera velia was grown in f/2 medium at 26 C in a 12/12 light/dark cycle with an intensity of 3.15 W m-2, which supported the growth of cystic but not motile stages. After about 14 days of cultivation, cells were collected by centrifugation. For separation of mt DNA from total cellular DNA, 0.5 mg of C. velia DNA was resolved in a mixed ultracentrifuge tube containing 8.5 ml TE, 9.5 g CsCl, and 400 mg Hoechst 33258. Tubes were spun at 45000 rpm at 25 C in a Beckman SW60Ti rotor for 72 hr, and DNA was ethanol-precipitated from individual fractions.
characteristics.characteristic: description
characteristics.text: Chromera velia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_505693
characteristics.characteristic: organism
characteristics.text: Chromera velia CCMP2878 mitochondrial DNA-enriched CsCl fraction
characteristics.characteristic: title