characteristics.text: SAMN00150248
characteristics.characteristic: External Id
characteristics.text: LIBEST_019617
characteristics.characteristic: External Id
characteristics.text: University of Wisconsin-Milwaukee
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:23.200Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Library created using PCR-Select cDNA Subtraction (Clonetech) using pooled liver and gonad of male and female adult fathead minnows and subtracting samples from fish exposed to dietary methylmercury (3.93 ( .08 ug g-1) versus controls. Subtractions were in both directions. The resultant pool of cDNA clones obtained from the PCR reaction were shotgun ligated into pGEM T-Easy cloning vector (Promega, Madison, WI) and then transformed into DH5a cells and plated onto Luria-Bertani (LB) agar plates containing ampicillin and oxacillin (100 ug/mL each).
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Pimephales promelas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_90988
characteristics.characteristic: organism
characteristics.text: male and female
characteristics.characteristic: sex
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: tissue-type
characteristics.text: Fathead minnow methylmercury SSH cDNA Library
characteristics.characteristic: title