characteristics.text: LIBEST_004119
characteristics.characteristic: External Id
characteristics.text: SAMN00158670
characteristics.characteristic: External Id
characteristics.text: Duke University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-05-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:50.900Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was constructed by John Davies and Jeffrey McDermott. RNA was isolated from strain CC-2290 (Minnesota isolate of C. reinhardtii) grown to mid-log phase in TAP (acetate containing) medium in the light. PolyA mRNA was purified, and cDNA was synthesized and directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al (1996) Genome Research 6: 791-806.
characteristics.characteristic: description
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: CC-2290 wild type mt- S1 D2
characteristics.characteristic: strain
characteristics.text: C. reinhardtii CC-2290, normalized, Lambda Zap II
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK-
characteristics.characteristic: vector