characteristics.text: LIBEST_008796
characteristics.characteristic: External Id
characteristics.text: SAMN00163316
characteristics.characteristic: External Id
characteristics.text: Kyowa Hakko Kogyo
characteristics.characteristic: INSDC center name
characteristics.text: 2002-05-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:17.580Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: HBdl12, derived from primary kidney epithelial cells
characteristics.characteristic: cell-line
characteristics.text: PCR-representational difference analysis was applied to mRNAs from two cell lines derived from primary neonatal rat kidney by immortalization with a wild type adenovirus E1A 12S cDNA or a mutant HBdl12, wherein the mutant derived cell line was the tester and the wild type the driver. Therefore the cDNAs activated or upregulated in the mutant were the targets. Differential expression was validated by RT-PCR and Northern analyses of the cDNAS obtained on the source mRNAs. The WT derived cells retained epithelial and mature kidney specific expression profiles. The HBdl12 derived cells have lost many such characteristics and have undergone epithelial mesenchymal transition (EMT) and have many characteristics of metanephric mesenchyme. The two cell types respond differently when exposed to V12ras.
characteristics.characteristic: description
characteristics.text: neonatal
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001372
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: M13 forward
characteristics.characteristic: re_1
characteristics.text: M13 reverse
characteristics.characteristic: re_2
characteristics.text: Fisher F344
characteristics.characteristic: strain
characteristics.text: Rat kidney: upregulated in EMT and tumor progression
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK(-)
characteristics.characteristic: vector