characteristics.text: SAMN00166581
characteristics.characteristic: External Id
characteristics.text: LIBEST_014469
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2003-10-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:55.840Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany).
characteristics.characteristic: description
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: whole embryo, pool of stages 10, 20 and 30
characteristics.characteristic: tissue-type
characteristics.text: XtSt10-30
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pRKW2
characteristics.characteristic: vector