characteristics.text: SAMN00155591
characteristics.characteristic: External Id
characteristics.text: LIBEST_001000
characteristics.characteristic: External Id
characteristics.text: Tokai University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1997-09-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:28.303Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA of Entamoeba histolytica HM-1:IMSS strain, trophozoite was prepared with QuickPrep Micro mRNA purification kit (Pharmacia). The first cDNA primed with a NotI-oligo(dT) primer, double-strand cDNA was ligated to EcoRI adapters with TimeSaver cDNA Synthesis Kit and Directional Cloning Toolbox (Pharmacia), and digested with NotI and cloned into the NotI and EcoRI sites of lambdaExCell (Pharmacia). Library was constructed by Tomoo Tanaka.
characteristics.characteristic: description
characteristics.text: trophozoite
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002592
characteristics.characteristic: dev-stage
characteristics.text: Entamoeba histolytica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5759
characteristics.characteristic: organism
characteristics.text: HM-1:IMSS
characteristics.characteristic: strain
characteristics.text: Entamoeba histolytica HM-1:IMSS mRNA (Tomoo Tanaka)
characteristics.characteristic: title