characteristics.text: SAMN00154804
characteristics.characteristic: External Id
characteristics.text: LIBEST_000189
characteristics.characteristic: External Id
characteristics.text: Wayne State University
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:05.457Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified from the mouse Ltk- fibroblastoid cultured cells. Double-stranded cDNAs were synthesized from the mRNA using an oligo(dT)-NotI primer. After shearing to 200-400 bp, a synthetic linker-primer, which has one blunt and one sticky end and an internal EcoRI site, was ligated to the cDNAs. The cDNAs were amplified by the polymerase chain reaction (PCR) using the ligated linker-primer sequence. After denaturation and reassociation of the ds-cDNAs, and isolation of single- stranded cDNAs by hydroxyapatite chromatography, the single- stranded cDNAs were again amplified by PCR. The cDNAs were digested with EcoRI and NotI, and inserted into a plasmid vector.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: C3H/An
characteristics.characteristic: strain
characteristics.text: Equalized cDNA library from Ltk- cultured cells
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector