characteristics.text: LIBEST_008975
characteristics.characteristic: External Id
characteristics.text: SAMN00163488
characteristics.characteristic: External Id
characteristics.text: National Institute of Diabetes and Digestive and Kidney Diseases
characteristics.characteristic: INSDC center name
characteristics.text: 2001-06-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:22.203Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Erythroid Cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000232
characteristics.characteristic: cell type
characteristics.text: Primary Culture of Peripheral Blood Mononuclear Cells
characteristics.characteristic: cell-line
characteristics.text: 65,000 proliferating erythroid cells from the buffy coat of a blood donation were obtained by flow cytometric separation after a 5-day culture period in the presence of erythropoietin. Total RNA was purified from the sorted cell population using TRIzol reagent. RNA (0.3 ug) was converted into double stranded cDNA using Clontech's CapFinder cDNA Library Construction Kit (Clontech) according to the manufacturer's protocol and cloned into EcoRI digested Lambda Zap II vector (Stratagene). The phage library was amplified once prior to in vivo excision in SOLR cells. Individual colonies were grown, and the cDNA inserts were sequenced in high throughput (NIH intramural sequencing center http://www.nisc.nih.gov/).
characteristics.characteristic: description
characteristics.text: Progenitor; EPO responsive CD71++++
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Hembase; Erythroid Progenitor Cells (LCB:ax library)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP II
characteristics.characteristic: vector