characteristics.text: SAMN00164576
characteristics.characteristic: External Id
characteristics.text: LIBEST_022341
characteristics.characteristic: External Id
characteristics.text: Massachusetts Institute Of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:41.807Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Whole animal
characteristics.characteristic: cell type
characteristics.text: 5' RACE product for ceh-30 (C33D12.7) from total RNA of a mixed stage population of wild-type (N2) C. elegans. Reverse transcription performed using a gene-specific primer whose sequence is given in the comments of the EST sequence file, tailed using terminal transferase with dCTP, and amplified by PCR using a second gene-specific primer whose sequence is given in the comments of the EST sequence file and the Gibco Abridged Anchor primer. Products were ligated into pGEM T-Easy. Individual transformants were picked, miniprepped, and sequenced using SP6 and T7 primers. This sequence file has been trimmed to remove nucleotides added by terminal transferase and to remove primer and vector sequences.
characteristics.characteristic: description
characteristics.text: Mixed stage population
characteristics.characteristic: dev-stage
characteristics.text: Whole animal
characteristics.characteristic: organ
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: N2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004028
characteristics.characteristic: strain
characteristics.text: Whole animal
characteristics.characteristic: tissue-type
characteristics.text: N2 Total RNA 5' RACE
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM T-Easy
characteristics.characteristic: vector