characteristics.text: SAMN00160196
characteristics.characteristic: External Id
characteristics.text: LIBEST_005658
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:39.060Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Puma (winter rye)
characteristics.characteristic: cultivar
characteristics.text: Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/(not packaged). cDNA is directly ligated into SK+/XhoI-EcoRI, then electroporated into TOP10 cells (Invitrogen).
characteristics.characteristic: description
characteristics.text: seedling three-leaf stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007106
characteristics.characteristic: dev-stage
characteristics.text: Secale cereale
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4550
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: leaf, crown
characteristics.characteristic: tissue-type
characteristics.text: Sc02_AAFC_ECORC_cold_stressed_winter_rye_seedlings
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Bluescript SK+/XhoI-EcoRI
characteristics.characteristic: vector