characteristics.text: SAMN00017029
characteristics.characteristic: External Id
characteristics.text: DOE JGI
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-16T19:27:32.753Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:58:17.447Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS085002
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS085002
characteristics.characteristic: SRA accession
characteristics.text: oxidative stress; H2O2
characteristics.characteristic: assay
characteristics.text: YPD
characteristics.characteristic: culture media
characteristics.text: For the oxidative stress experiments, a saturated overnight culture of strain SC5314 was diluted into 800 mls of YPD medium to an OD600 of 0.1 and allowed to grow at 30*C until the culture reached an OD600 of ~1.0. At this point the culture was split into three 250ml cultures in separate 1L flasks. To these cultures, hydrogen peroxide (H2O2) was added to a final concentration of 5mM (high oxidative stress), 0.5mM (low oxidative stress) or omitted (untreated control). After 15 minutes of treatment at 30*C, the cells were harvested by vacuum filtration and immediately stored at -80*C.
characteristics.characteristic: description
characteristics.text: Candida albicans SC5314
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_237561
characteristics.characteristic: organism
characteristics.text: SC5314
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006481
characteristics.characteristic: strain
characteristics.text: C. albicans SC5314 grown under low oxidative stress
characteristics.characteristic: title