characteristics.text: LIBEST_003887
characteristics.characteristic: External Id
characteristics.text: SAMN00158440
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2000-03-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:45.063Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Medicago truncatula genotype A17
characteristics.characteristic: cultivar
characteristics.text: cDNA was prepared from polyA+ enriched RNA from roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
characteristics.characteristic: description
characteristics.text: Roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme. The library was made from a mixture of RNA from each of these stages.
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Medicago truncatula/Glomus versiforme mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_119092
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: roots colonized with Glomus versiforme
characteristics.characteristic: tissue-type
characteristics.text: MHAM
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector