characteristics.text: SAMN00152949
characteristics.characteristic: External Id
characteristics.text: LIBEST_021206
characteristics.characteristic: External Id
characteristics.text: Northwest A&F University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:12.307Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: young leaves
characteristics.characteristic: cell type
characteristics.text: Xiaoyan 22
characteristics.characteristic: cultivar
characteristics.text: Total RNA was extracted by Trizol, reverse transcription was conducted by A-MLV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.
characteristics.characteristic: description
characteristics.text: stage of 4-5 leaves
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: SSH-cDNA Library after serious drought in leaves of wheat
characteristics.characteristic: title