characteristics.text: LIBEST_015631
characteristics.characteristic: External Id
characteristics.text: SAMN00154091
characteristics.characteristic: External Id
characteristics.text: Universite Laval
characteristics.characteristic: INSDC center name
characteristics.text: 2004-04-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:28.987Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Actively growing shoot tips of trees subjected to fertilization with 0 or 25 mM ammonium nitrate for 1, 3, or 7 days, or removal of a ring of bark from the main stem (stem girdling) for 1 or 6 days, or treatment with different sources of nitrogen and carbon for 4 or 24 hours. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation
characteristics.characteristic: description
characteristics.text: E. coli DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Shoot apex, primary stem and developing leaves up to and including LPI 1
characteristics.characteristic: organ
characteristics.text: Populus trichocarpa x Populus deltoides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3695
characteristics.characteristic: organism
characteristics.text: Eco-RI
characteristics.characteristic: re_1
characteristics.text: Xho-I
characteristics.characteristic: re_2
characteristics.text: MALE
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Shoot tips of trees approximately 80-120 cm tall
characteristics.characteristic: tissue-type
characteristics.text: GQ012
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector