characteristics.text: LIBEST_010849
characteristics.characteristic: External Id
characteristics.text: SAMN00152819
characteristics.characteristic: External Id
characteristics.text: National Center for Coffee Research - CENICAFE
characteristics.characteristic: INSDC center name
characteristics.text: 2002-05-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:15.573Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Caturra
characteristics.characteristic: cultivar
characteristics.text: The cDNA library was constructed from mRNA isolated from young coffee leaves. Complementary DNA was synthesized from mRNA using the primer oligo-dT/CDSIII (3' priming) and smart oligonucleotide that attaches at the 5' end during elongation with reverse transcriptase. The library is non-directional. Cycle sequencing of individual clones was performed and the samples run on an ABI377 sequencing machine. The project was a collaborative research initiative between the Colombian National Center for Coffee Research - CENICAFE and the laboratory directed by Dr. Susan McCouch, Department of Plant Breeding, Cornell University, Ithaca, NY. This research was partially financed by COLCIENCIAS-Instituto Colombiano para el Desarrollo de la Ciencia
characteristics.characteristic: description
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Coffea arabica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13443
characteristics.characteristic: organism
characteristics.text: Coffee leaves PCRScript library
characteristics.characteristic: title
characteristics.text: PCRScript
characteristics.characteristic: vector