characteristics.text: LIBEST_023720
characteristics.characteristic: External Id
characteristics.text: SAMN00166678
characteristics.characteristic: External Id
characteristics.text: Ministry of Education, Huazhong Agricultural University P.R.C.
characteristics.characteristic: INSDC center name
characteristics.text: 2008-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:24.640Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: P. cubensis resistance cucumber inbred material
characteristics.characteristic: cultivar
characteristics.text: Pseudoperonospora cubensis (Berk. and Curk.) Rostov (P. cubensis)-challenged leaf Suppression Subtractive Hybridization cDNA library of cucumber seedlings. Detached leaves were challenged with 1x105 sporangia/ml P. cubensis. Leaf tissue was collected at 32, 48, 56 hours post-challenge respectively, and frozen in liquid nitrogen immediately. Detached leaves sprayed with water were used as controls.
characteristics.characteristic: description
characteristics.text: two second leaf stage
characteristics.characteristic: dev-stage
characteristics.text: IL57
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003292
characteristics.characteristic: lab-host
characteristics.text: Cucumis sativus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3659
characteristics.characteristic: organism
characteristics.text: SmalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: SSH cDNA library of cucumber seedlings challenged with P. cubensis
characteristics.characteristic: title
characteristics.text: BlueScript M13 (+) SK
characteristics.characteristic: vector