characteristics.text: SAMN00157091
characteristics.characteristic: External Id
characteristics.text: LIBEST_002532
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1999-11-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:09.717Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was prepared from 2ug of poly A+ RNA. EcoRI-XhoI cut cDNA was then ligated into UniZap-XR (Stratagene) with EcoRI at the 5' end and XhoI at the 3' end. SS-library phagemids were prepared by mass excision from the original library and normalized by hybridization to biotinylated driver (prepared from the same library by PCR) to Cot-omega of 11. After removal of hybrids and excess driver by streptavidin sepharose chromatography, the ss-phagemids were made double stranded and electroporated into Top-10 F'. Original library contruction by Bruce Blumberg (Blumberg et al., 1991 Science 253, 194-196; Hawlet et al., 1995, Genes Dev. 9, 2923-2935). Normalized by Jihwan Song (Song, Cho and Blumberg, unpublished). Note: This is a Xenopus Gene Collection (XGC) library.
characteristics.characteristic: description
characteristics.text: Top-10 F'
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: oocyte (stages 5 and 6)
characteristics.characteristic: tissue-type
characteristics.text: Xenopus laevis oocyte
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector