characteristics.text: LIBEST_010165
characteristics.characteristic: External Id
characteristics.text: SAMN00153761
characteristics.characteristic: External Id
characteristics.text: Instituto Butantan
characteristics.characteristic: INSDC center name
characteristics.text: 2002-05-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:57.090Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 5ug of mRNA from Bothrops insularis venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (LifeTechnologies). The cDNAs were selected by size (350-600 pb and up 600 pb) in agarose gel electrophoresis, linked to SalI adapters and directionally cloned in pSPORT-1 vector (LifeTechnologies). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).
characteristics.characteristic: description
characteristics.text: venom glands
characteristics.characteristic: organ
characteristics.text: Bothrops insularis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8723
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: venom glands
characteristics.characteristic: tissue-type
characteristics.text: Snake Bothrops insularis library IL1
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT-1
characteristics.characteristic: vector