characteristics.text: SAMN00156020
characteristics.characteristic: External Id
characteristics.text: LIBEST_001442
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:07.390Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:21:44.317Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: pituitary cell line
characteristics.characteristic: cell-line
characteristics.text: Double-stranded cDNA was prepared from cell line AtT-20 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This cell line was engineered for inducible overexpression of RESP18 using the rTET system. Cells were induced such that RESP18 protein would traverse the ER to the Golgi and induce the RESP18-activated signaling pathway. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
characteristics.characteristic: description
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: pituitary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000007
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Schiller mouse AtT20 RESP induced
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK- (Stratagene)
characteristics.characteristic: vector