characteristics.text: LIBEST_000548
characteristics.characteristic: External Id
characteristics.text: SAMN00155153
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1996-11-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:14.110Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a XhoI - oligo(dT) primer. Double-stranded cDNA was ligated to EcoRI adaptors digested with XhoI and cloned into XhoI and EcoRI sites. Primary complexity of the library was 10 in the 7th. The library went through one round of amplification.
characteristics.characteristic: description
characteristics.text: mixed stages (embryo to adult)
characteristics.characteristic: dev-stage
characteristics.text: not applicable (host cell line)
characteristics.characteristic: lab-host
characteristics.text: Pristionchus pacificus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_54126
characteristics.characteristic: organism
characteristics.text: 5' EcoRI
characteristics.characteristic: re_1
characteristics.text: 3' XhoI
characteristics.characteristic: re_2
characteristics.text: predominantly hermaphroditic
characteristics.characteristic: sex
characteristics.text: PS 312
characteristics.characteristic: strain
characteristics.text: Sommer Pristionchus
characteristics.characteristic: title
characteristics.text: Phagemid (Ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: Uni-ZAP XR Vector (Stratagene)
characteristics.characteristic: vector