characteristics.text: SAMN00150639
characteristics.characteristic: External Id
characteristics.text: LIBEST_020050
characteristics.characteristic: External Id
characteristics.text: Cancer Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:35.447Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The mRNA from the liver of Andrias davidianus was purified and the first-strand cDNA was synthesized by reverse transcription with SMART Oligonucleotide (5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCGGG-3') and CDS 3' PCR Primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3', N= A, G, C, or T; N-1=A, G, or C). The SMART Oligonucleotide and the CDS 3' PCR Primer contained Sfi IA and Sfi IB restriction enzyme sites respectively. The first-strand cDNA was used as a template to amplify the double-strand cDNA by long-distance PCR (LD-PCR) with 5' PCR primer (5'-AAGCAGTGGTATCAACGCAGAGT-3') and the CDS 3' PCR Primer. The product was purified and digested by Sfi I (IA and IB) restriction enzyme. The purified double-strand cDNA was ligated into the Sfi I-digested lambdaTripIEx2 vector and the recombinant DNA was packaged in vitro. The phages were transducted to bacteria XL1-Blue and then were amplified.
characteristics.characteristic: description
characteristics.text: XL1-Blue E.coli
characteristics.characteristic: lab-host
characteristics.text: Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Andrias davidianus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_141262
characteristics.characteristic: organism
characteristics.text: Sfi IA
characteristics.characteristic: re_1
characteristics.text: Sfi IB
characteristics.characteristic: re_2
characteristics.text: cDNA Library from the liver of Andrias davidianus
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: lambdaTripIEx2
characteristics.characteristic: vector