characteristics.text: SAMN00138214
characteristics.characteristic: External Id
characteristics.text: University of Alberta, Edmonton, Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2011-01-24T12:05:48.717Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T09:00:58.850Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS139115
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS139115
characteristics.characteristic: SRA accession
characteristics.text: One-year-old jack pine seedlings from north-western Ontario were supplied by PRT nurseries. Seedlings were transplanted into 3.78 L pots containing sunshine mix #4 (Sun Gro Horticulture Canada), and were subject to treatment during their second growth cycle. The plants were watered every two to three days in a growth chamber under the following conditions: 20 *C, 20-25 % humidity, 0.5 g/L fertilizer (20-20-20) twice per week, and full-spectrum fluorescent lighting lamps for 16 h light / 8 h dark cycle. At the onset of the experiment, trees were either (1) exposed to water deficit conditions by withholding water for 2 weeks prior to harvest, or (2) wounded by incising the bark with a razor blade every 5 mm along the stem along two opposite sides, and harvesting 1, 2, 4, or 8 days following the wound treatment. Needles, whole stems, and roots were rapidly harvested. A total of 10 seedlings (1-2 replicates per treatment) were harvested, and each replicate is assumed to be a distinct genotype. Harvested tissues were frozen in liquid nitrogen immediately following collection and stored at -80 *C until RNA extraction.
characteristics.characteristic: description
characteristics.text: Pinus banksiana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3353
characteristics.characteristic: organism
characteristics.text: Jack pine
characteristics.characteristic: title