characteristics.text: SAMN00150221
characteristics.characteristic: External Id
characteristics.text: LIBEST_016461
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2004-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:53.023Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: H11-11
characteristics.characteristic: cultivar
characteristics.text: Sapling trees were grown to 30cm to 50cm in height in aerated hydroponic growth media under growth chamber conditions. Roots were harvested from untreated control trees at 0 hours, as well as from trees grown in media without a nitrogen source for 24 hours and 48 hours. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.
characteristics.characteristic: description
characteristics.text: E. coli DH10B T1 phage resistant cells
characteristics.characteristic: lab-host
characteristics.text: Populus trichocarpa x Populus deltoides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3695
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: PTxD-NR-A-8
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector