characteristics.text: LIBEST_005410
characteristics.characteristic: External Id
characteristics.text: SAMN00159948
characteristics.characteristic: External Id
characteristics.text: Texas Children's Cancer Center and Human Genome Sequencing Center at Baylor College of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:29.860Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: myeloid cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000763
characteristics.characteristic: cell type
characteristics.text: First strand cDNA was primed with an anchored XhoI-oligo(dT) primer [5'GGAGGACTCGAGCGGCCGCAGGAGGAG(T)VN 3'; V=A,C,G; N=A,C,G,T] and then dG tailed. Second strand was primed with a BamH1-dC primer [5'AGAGAGCTCGGATCCGCGGCCGCAATAATAATAAT(C) 3']. Double-stranded cDNA was then digested with BamH1 and XhoI and directionally cloned into the BamH1 and Sa1I sites of lambda pSB vector. Library went through one round of normalization. Library was constructed by Wei Yu at RIKEN of Japan (Carninci P, Westover A, Nishiyama Y, Ohsumi T, Itoh M, Nagaoka S, SasakiN, Okazaki Y, Muramatsu M, Schneider C, Hayashizaki Y, High efficiency selection of full-length cDNA by improved biotinylated cap trapper., DNA Res 4: 1, 61-6, Feb 28, 1997)
characteristics.characteristic: description
characteristics.text: pediatric 6 years
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: leukopheresis
characteristics.characteristic: tissue-type
characteristics.text: Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA
characteristics.characteristic: title
characteristics.text: lambda pSB
characteristics.characteristic: vector