characteristics.text: LIBEST_008674
characteristics.characteristic: External Id
characteristics.text: SAMN00163200
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:13.633Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-Oligo(dT) PCR-based library. Strongyloides_ratti_L3_adult cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. Mark Viney of the University of Bristol, UK.
characteristics.characteristic: description
characteristics.text: L3/adult
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Strongyloides ratti
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_34506
characteristics.characteristic: organism
characteristics.text: Strongyloides ratti L3 adult SL1 Topo1 Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid (amp. or kan. resistant)
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector