characteristics.text: LIBEST_017806
characteristics.characteristic: External Id
characteristics.text: SAMN00166619
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2005-08-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:35.380Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was made from dT primed cDNA and cloned into Invitrogen pCMVsport6 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCMVsport6 vector.
characteristics.characteristic: description
characteristics.text: Metamorphic (st.64)
characteristics.characteristic: dev-stage
characteristics.text: ElectroMAX DH10B
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: NIH_XGC_tropMet9
characteristics.characteristic: title
characteristics.text: pCMVsport6
characteristics.characteristic: vector