characteristics.text: SAMN00166584
characteristics.characteristic: External Id
characteristics.text: LIBEST_015540
characteristics.characteristic: External Id
characteristics.text: CNRS UMR 8080
characteristics.characteristic: INSDC center name
characteristics.text: 2004-03-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-08-29T21:03:14.897Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).
characteristics.characteristic: description
characteristics.text: stage 25-35
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007106
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Ivory Coast
characteristics.characteristic: strain
characteristics.text: pool of heads and retinas from tailbud stages 25-35
characteristics.characteristic: tissue-type
characteristics.text: Xenopus tropicalis xthr plasmid library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSPORT6_xthr
characteristics.characteristic: vector