characteristics.text: SAMN00012273
characteristics.characteristic: External Id
characteristics.text: Yonsei University
characteristics.characteristic: INSDC center name
characteristics.text: 2011-02-23T11:46:57.240Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:53:57.500Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS059101
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS059101
characteristics.characteristic: SRA accession
characteristics.text: A multipronged approach comprising microcosm studies, Titanium pyrosequencing, 16S rRNA gene clone libraries, and dechlorinator-targeted quantitative real-time PCR (qPCR) characterized reductive dechlorinating activities and populations in tidal flat sediments collected from South Korea’s central west coast near Kangwha. In microcosms established with surface sediments, PCE dechlorination to TCE began within 10 days and 100% of the initial amount of PCE was converted to TCE after 37 days. cis-1,2-dichloroethene (cis-DCE) was observed as dechlorination end product in microcosms containing sediments collected from deeper zones (i.e., 35-40 cm below ground surface). Pyrosequencing of bacterial 16S rRNA genes and 16S rRNA gene-targeted qPCR results revealed Desulfuromonas michiganensis-like populations predominanted in both TCE and cis-DCE producing microcosms. Other abundant groups included Desulfuromonas thiophila and Pelobacter acidigallici-like populations in the surface sediment microcosms, and Desulfovibrio dechloracetivorans and Fusibacter paucivorans-like populations in the deeper sediment microcosms. Dehalococcoides spp. populations were undetected in these sediments before and after incubation with PCE.
characteristics.characteristic: description
characteristics.text: marine metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_408172
characteristics.characteristic: organism
characteristics.text: Increased populations during PCE to TCE or cis-DCE dechlorination
characteristics.characteristic: title