characteristics.text: SAMN00165954
characteristics.characteristic: External Id
characteristics.text: LIBEST_023385
characteristics.characteristic: External Id
characteristics.text: National Institute of Agricultural Biotechnology
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:13.710Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Yeoung San
characteristics.characteristic: cultivar
characteristics.text: The embryo 35 DAF was used to prepare RNA (driver) and embryo 10 DAF was used to prepare RNA (tester). Suppression subtractive hybridization was performed according to the manufacturer's instructions using the polymerase chain reaction (PCR)-based cDNA subtraction kit (Clontech, Mountain View, CA, USA). The PCR products were ligated into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) to generate the subtracted cDNA library.
characteristics.characteristic: description
characteristics.text: Embryo 10 and 35 DAF (days after flowering)
characteristics.characteristic: dev-stage
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Immature seed
characteristics.characteristic: tissue-type
characteristics.text: Suppression subtractive hybridization cDNA library from embryo 10 DAF (Ref. embryo 35 DAF)
characteristics.characteristic: title
characteristics.text: pCR 2.1-TOPO
characteristics.characteristic: vector