characteristics.text: LIBEST_000119
characteristics.characteristic: External Id
characteristics.text: SAMN00154734
characteristics.characteristic: External Id
characteristics.text: INSERM
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:03.493Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between EcoRV and NotI sites of PGEM 5zf(+). The 3' end is at the NotI site. The EcoRV site is lost during the cloning procedure. cDNA corresponding to abundant species were eliminated from this library.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRV
characteristics.characteristic: re_1
characteristics.text: Not1
characteristics.characteristic: re_2
characteristics.text: Testis 1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: PGEM 5zf(+)
characteristics.characteristic: vector