characteristics.text: SAMN00164371
characteristics.characteristic: External Id
characteristics.text: LIBEST_009868
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.897Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PolyA mRNA from partially-sporulated oocysts was converted to cDNA using the template-switching PCR method (SMART cDNA, Clontech Inc.,) and sized selected on SizeSep 400 columns (Amersham Pharmacia Biotech Inc.). First strand was reverse transcribed using the CDS III-oligo-dT primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector containing directional SfiI sites, and electroporated into DH10B or DH12S cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides. Library Source: Michael White, Maria E. Jerome,Emily A.Johnson, Jay A. Radke, Montana State University. Clone Availability: David Sibley, Washington University
characteristics.characteristic: description
characteristics.text: Partially sporulated oocysts
characteristics.characteristic: dev-stage
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: BamH1
characteristics.characteristic: re_1
characteristics.text: EcoR1
characteristics.characteristic: re_2
characteristics.text: VEG (Type III)
characteristics.characteristic: strain
characteristics.text: TgVEG Partially sporulated oocyst cDNA
characteristics.characteristic: title
characteristics.text: Modified pBluescript (pBSSK+)
characteristics.characteristic: vector