characteristics.text: SAMN00155028
characteristics.characteristic: External Id
characteristics.text: LIBEST_000417
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1995-12-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:10.667Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (ampicillin resistant)
characteristics.characteristic: lab-host
characteristics.text: parathyroid gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001132
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: parathyroid tumor
characteristics.characteristic: tissue-type
characteristics.text: Soares_parathyroid_tumor_NbHPA
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3D (Pharmacia) with a modified polylinker
characteristics.characteristic: vector