characteristics.text: LIBEST_009535
characteristics.characteristic: External Id
characteristics.text: SAMN00164048
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-06-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:40.127Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Mark Viney of Bristol, UK.
characteristics.characteristic: description
characteristics.text: L3/adult
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Strongyloides ratti
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_34506
characteristics.characteristic: organism
characteristics.text: Strongyloides ratti L3 adult pAMP1 v1 Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAMP1 (Gibco)
characteristics.characteristic: vector