characteristics.text: LIBEST_008753
characteristics.characteristic: External Id
characteristics.text: SAMN00163278
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2003-03-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:16.250Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Pancreatic endocrine tumor
characteristics.characteristic: cell type
characteristics.text: GKP4
characteristics.characteristic: cell-line
characteristics.text: mRNA from GKP2 and GKP4 cells was used to perform subtraction suppression hybridization. Genes that are more highly expressed in GKP4 cells (a precursor cell line), compared to GKP2 cells (an insulinoma derived cell) were cloned pCR 2.1-TOPO and transformed into TOP10 competent bacteria.
characteristics.characteristic: description
characteristics.text: TOP10 (Invitrogen, Carlsbad, CA)
characteristics.characteristic: lab-host
characteristics.text: Pancreas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001264
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: BamH1
characteristics.characteristic: re_2
characteristics.text: C57BL/6J x DBA/2
characteristics.characteristic: strain
characteristics.text: GKP4 subtraction cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR 2.1-TOPO (Invitrogen, Carlsbad, CA)
characteristics.characteristic: vector