characteristics.text: LIBEST_022203
characteristics.characteristic: External Id
characteristics.text: SAMN00154568
characteristics.characteristic: External Id
characteristics.text: The J. Craig Venter Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-11-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:38.500Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A17
characteristics.characteristic: cultivar
characteristics.text: Tissue: low-phosphate treated root, Glomus versiforme infected root, uninoculated root, Sinorhizobium meliloti T202 inoculated root and nodules (3-11 DAI), Sinorhizobium meliloti 102F51 inoculated root and nodules (3-21 DAI), Sinorhizobium meliloti 1021 inoculated root and nodules (28 DAI). Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/t2.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Maria Harrison, Sue Miller, Carrol Vance and Vagner Benedito.
characteristics.characteristic: description
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: mixed root and nodules
characteristics.characteristic: tissue-type
characteristics.text: JCVI-MT3
characteristics.characteristic: title
characteristics.text: pDNR-LIB (Clontech)
characteristics.characteristic: vector