characteristics.text: SAMN00166074
characteristics.characteristic: External Id
characteristics.text: LIBEST_014188
characteristics.characteristic: External Id
characteristics.text: National Institute for Agricultural Research (INRA)
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:48.073Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A composite cDNA library was made with mRNA isolated from opposite and tension wood tissues corresponding to the mature xylem collected after debarking the stem and pealing the young differentiating xylem with a scalpel. These tissues were collected on 3 different tilted trees grown in the nursery. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech, SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)
characteristics.characteristic: description
characteristics.text: 3-years-old poplar trees grown in the nursery
characteristics.characteristic: dev-stage
characteristics.text: Populus tremula x Populus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80863
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: clone 717-1-B4
characteristics.characteristic: strain
characteristics.text: mature xylem harvested on opposite and tension wood stem
characteristics.characteristic: tissue-type
characteristics.text: Poplar cDNA library from mature xylem
characteristics.characteristic: title