characteristics.text: SAMN00164506
characteristics.characteristic: External Id
characteristics.text: LIBEST_022271
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:39.930Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into pDONR222 (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with a biotin-attB2-Oligo(dT) primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp., Carlsbad, CA). The second strand was synthesized and an attB1 adaptor ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCCC-3'). cDNA was size selected from 0.5-1kb using 1% agarose gel electrophoresis and then inserted into the vector using site specific recombination (flanking attB sites on cDNA). Library constructed with RNA harvested from nitrogen limited cells at the Alfred Wegener Institute and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: asexual and sexual stages mixed
characteristics.characteristic: dev-stage
characteristics.text: DH10B T1 Phage resistant
characteristics.characteristic: lab-host
characteristics.text: Aureococcus anophagefferens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_44056
characteristics.characteristic: organism
characteristics.text: attB1
characteristics.characteristic: re_1
characteristics.text: attB2
characteristics.characteristic: re_2
characteristics.text: CCMP1984
characteristics.characteristic: strain
characteristics.text: single cell plant material
characteristics.characteristic: tissue-type
characteristics.text: CATU Aureococcus anophagefferens Single cell plant material, asexual and sexual stages mixed
characteristics.characteristic: title
characteristics.text: pDONR222
characteristics.characteristic: vector