characteristics.text: LIBEST_000554
characteristics.characteristic: External Id
characteristics.text: SAMN00155159
characteristics.characteristic: External Id
characteristics.text: Queensland Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 1996-11-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:14.240Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Messenger RNA was isolated directly from lysates of schistosome miracidia using oligo-dT chromatography. First stand synthesis was primed with an oligo-dT-Xho-1 primer, and synthesized using M-MLV reverse transcriptase. Second strand synthesis was accomplished with RNaseH and T4 DNA polymerase. The double stranded cDNA was ligated to EcoR1 linkers, subsequently cleaved with EcoR1 and Xho1, and then ligated into phagemid lambda ZAP 11 XR
characteristics.characteristic: description
characteristics.text: Miracidium
characteristics.characteristic: dev-stage
characteristics.text: Mouse
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10088
characteristics.characteristic: lab-host
characteristics.text: Schistosoma japonicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6182
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xhol I
characteristics.characteristic: re_2
characteristics.text: Mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Chinese (Anhui) strain
characteristics.characteristic: strain
characteristics.text: Whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Miracidia Sjc 3/96
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP-II XR
characteristics.characteristic: vector