characteristics.text: LIBEST_007105
characteristics.characteristic: External Id
characteristics.text: SAMN00161638
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-11-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:26.403Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was constructed by Steve Fogarty, Robert Cole, and Keliang Tang at Washington University. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming, size-selected and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B (GeneHog, Research Genetics, Inc.) for sequencing. WARNING:the library may contain a small percentage of contaminents from human fibroblast cells.
characteristics.characteristic: description
characteristics.text: Tachyzoite
characteristics.characteristic: dev-stage
characteristics.text: DH10B (GeneHog, Research Genetics, Inc.)
characteristics.characteristic: lab-host
characteristics.text: Neospora caninum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29176
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Nc-1
characteristics.characteristic: strain
characteristics.text: Nc 1314 Tachyzoite cDNA
characteristics.characteristic: title
characteristics.text: pBluescript SK-
characteristics.characteristic: vector