characteristics.text: SAMN00153457
characteristics.characteristic: External Id
characteristics.text: LIBEST_021805
characteristics.characteristic: External Id
characteristics.text: MPI for Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:27.890Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: six randomly chosen genotypes from a wild population at ambient winter temperature of 4C. lat_lon: 54.68 N 10 E. collection_date: Feb-2004. Total RNA was extracted from meristematic region of plants collected in wild by SCUBA at ambient temperature of 4C in February 2004. Subsequent storage of 1 day in ambient water of whole plants at 6C prior to library preparation. Cleaned tissue was shock frozen in liq N2 and stored at -80C prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial PCR amplification of 18-25 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Sequencing based on Plasmid preps using the primer GTA AAA CGA CGG CCA GT at Max-Planck-Institute for Limnology, Dept Ecophysiology, August-Thienemann-Str. 2, 24306 Ploen, Germany, Contact for clones: treusch@uni-muenster.de, Institut for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149 Muenster, Germany.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Zostera marina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29655
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: meristem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009013
characteristics.characteristic: tissue-type
characteristics.text: ZM_A
characteristics.characteristic: title