characteristics.text: SAMN00155122
characteristics.characteristic: External Id
characteristics.text: LIBEST_000515
characteristics.characteristic: External Id
characteristics.text: National Human Genome Research Institute/NIH
characteristics.characteristic: INSDC center name
characteristics.text: 1996-10-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:13.283Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: HeLa cell line; ATCC
characteristics.characteristic: cell-line
characteristics.text: cDNA was generated from cytoplasmic RNA using a mixture of random DNA hexamers and oligo(dT). From this pool of cDNA, human chromosome 7-enriched cDNA was isolated by direct cDNA selection using chromosome 7 genomic DNA (cosmids). The resulting direct-selected cDNA was cloned into a plasmid vector using a non-directional uracil DNA glycosylase (UDG)-mediated cloning strategy.
characteristics.characteristic: description
characteristics.text: E. coli strain DH5 alpha
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Chromosome 7 HeLa cDNA Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAMP10
characteristics.characteristic: vector