characteristics.text: LIBEST_015367
characteristics.characteristic: External Id
characteristics.text: SAMN00152269
characteristics.characteristic: External Id
characteristics.text: Institute of Plant Biology, National Taiwan University
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-03T23:44:05.277Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:16:42.793Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hawaii7996
characteristics.characteristic: cultivar
characteristics.text: Plant samples were provided by Dr. Jaw-Fen Wang (Tainan, AVRDC). Each plant grown in a 3-inch pot was inoculated with 30 ml (108cfu/ml) of Rs strain Pss4 to give a final concentration of 108cfu/g soil. Roots and collars of inoculated and control plants were harvested 24 and 48 hours post inoculation (e.g. 24-control, 24-inoculated, 48-control and 48-inoculated).Poly(A) RNA was purified from total RNA specimen isolated from individual samples. The poly(A) RNA specimen prepared from 24-control and 48-control samples were mixed at 1:1 ratio. The poly(A) RNA specimen prepared from 24-inoculated and 48-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library LERSF was then constructed by subtracting the pooled control specimen from the pooled inoculated specimen using CLONTECHPCR-Select cDNA Subtraction kit.
characteristics.characteristic: description
characteristics.text: Three weeks old
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: root and collar
characteristics.characteristic: tissue-type
characteristics.text: Subtractive cDNA library prepared from tomato infected with Ralstonia solanacearum (Rs-Ck)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T EZ
characteristics.characteristic: vector