characteristics.text: LIBEST_005572
characteristics.characteristic: External Id
characteristics.text: SAMN00160110
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:36.550Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Dan Howe, University of Kentucky. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming and directionally cloned into the Uni-ZAP XR lambda vector. The library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B cells for sequencing. WARNING: the library contains a small percentage of cDNAs derived from the bovine host cells.
characteristics.characteristic: description
characteristics.text: merozoite
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Sarcocystis neurona
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_42890
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Sn3
characteristics.characteristic: strain
characteristics.text: cSn 1 S neurona invitro merozoite cDNA
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector