characteristics.text: LIBEST_016853
characteristics.characteristic: External Id
characteristics.text: SAMN00166589
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2005-01-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:05.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Gastrula library constructed by Russell B. Fletcher in R. Harland's lab using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). SalI (5' end) -NotI (3' end) cDNA was inserted into vector pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html).
characteristics.characteristic: description
characteristics.text: Gastrula (st. 10.5-12.5)
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1-Blue derivative, Stratagene ElectroTen-Blue
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: NIH_XGC_tropGas7
characteristics.characteristic: title
characteristics.text: pCS108
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002843
characteristics.characteristic: vector