characteristics.text: SAMN00150219
characteristics.characteristic: External Id
characteristics.text: LIBEST_016459
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2004-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:52.977Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: H11-11
characteristics.characteristic: cultivar
characteristics.text: Sapling trees one metre in height and grown under greenhouse conditions were exposed to continuous feeding by Malacosoma disstria Hubner (forest tent caterpillar) mid-instar larvae caged on the sapling using mesh bags. Mature leaves from within the caged region were collected 4 hours, 8 hours and 24 hours after the onset of treatment. Mature leaves were also collected above the caged region (systemic response) 4 hours, 12 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.
characteristics.characteristic: description
characteristics.text: E. coli DH10B T1 phage resistant cells
characteristics.characteristic: lab-host
characteristics.text: Populus trichocarpa x Populus deltoides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3695
characteristics.characteristic: organism
characteristics.text: SstI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: PTxD-IL-FL-A-4
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector