characteristics.text: LIBEST_017609
characteristics.characteristic: External Id
characteristics.text: SAMN00154227
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2005-07-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:29.703Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 31 from Mother Tree 7-56
characteristics.characteristic: cell type
characteristics.text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA
characteristics.characteristic: cultivar
characteristics.text: Library enriched in transcripts present in suspension culture of Loblolly Pine (Pinus taeda L.), 7-56. Suspension culture was established from fertilized megagametophytes, according to method of Pullman et al 2003 (Pullman, G., S. Johnson, G. Peter, J. Cairney, and N. Xu. 2003. Improving loblolly pine somatic embryo maturation: Comparison of somatic and zygotic embryo morphology, germination, and gene expression. Plant Cell Reports 21:747-758. ) This suspension cell culture, which contains early developing embryos and undifferentiated embryogeneic tissue, was the source of the RNA used for the suspension culture library. Subtracted from this was cDNA made from a pool of RNA from zygotic embryos and female gametophytes from all stages of development plus somatic embryos from developmental stages beyond susepension culture (see Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 [http://www.plantphysiol.org/cgi/content/full/127/4/1556]) .
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Suspension Culture Somatic Embryos (Stages 1-3)
characteristics.characteristic: organ
characteristics.text: Pinus taeda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3352
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: ClaI
characteristics.characteristic: re_2
characteristics.text: Suspension Culture Somatic Embryos (Stages 1-3)
characteristics.characteristic: tissue-type
characteristics.text: Normalized pine embryo library, Lib_D
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGADT7-RecAB
characteristics.characteristic: vector