characteristics.text: LIBGSS_002962
characteristics.characteristic: External Id
characteristics.text: SAMN00151938
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:39:11.253Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Faribault
characteristics.characteristic: cultivar
characteristics.text: BAC end subclones were prepared from selected BACs within the UMN BAC library (Danesh et al, Theor. Appl. Genet. 96:196, 1998), which was constructed using the EcoR I site of pECSBAC4. The library consists of 72,960 clones with an average insert size of 120 Kb, equal to 7 haploid genome equivalents. Screening of the library is done by hybridization of high-density colony filters and/or PCR amplification of DNA pools. Four high density filters, each containing 18,432 clones (doubly spotted), represent the whole library for colony screening.
characteristics.characteristic: description
characteristics.text: cotyledon stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007049
characteristics.characteristic: dev-stage
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Sph I
characteristics.characteristic: re_2
characteristics.text: cotyledon leaves
characteristics.characteristic: tissue-type
characteristics.text: BAC end subclones (pCR2.1-TOPO, EcoR I, Sph I) from UMN pECSBAC4 EcoR I Soybean BAC Genomic Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1-TOPO
characteristics.characteristic: vector