characteristics.text: LIBEST_009545
characteristics.characteristic: External Id
characteristics.text: SAMN00164058
characteristics.characteristic: External Id
characteristics.text: Crop and Food Research
characteristics.characteristic: INSDC center name
characteristics.text: 2002-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:40.343Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Pukekohe Longkeeper
characteristics.characteristic: cultivar
characteristics.text: A. cepa sterilized seeds were germinated for 72h at 25 C before being transferred to hydroponic solution (Randle et al., 1995). The hydroponic solution was changed at 2 weekly intervals and after 8 weeks half of the plants were subjected to a 0% sulfur hydroponic solution for 48 hrs. RNA was extracted from root tissue of plants from both treatments. cDNAs differentially expressed under sulfur starvation were produced from polyA+ enriched RNA using the Clontech PCR-Select cDNA Subtraction Kit. The cDNAs were ligated into pGEM-T Easy vector from Promega and clones were randomly chosen for sequencing.
characteristics.characteristic: description
characteristics.text: Before bulbing
characteristics.characteristic: dev-stage
characteristics.text: DH5 alpha
characteristics.characteristic: lab-host
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Allium cepa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4679
characteristics.characteristic: organism
characteristics.text: Sulfur-deprived onion root library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T Easy
characteristics.characteristic: vector