characteristics.text: LIBEST_023460
characteristics.characteristic: External Id
characteristics.text: SAMN00166354
characteristics.characteristic: External Id
characteristics.text: UMR INRA/Universite Henri Poincare 1136
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:15.597Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The experimental site is a 100 year-old oak forest with a continuous canopy and a hornbeam understory (Quercus petraea (Mattuschka) Liebl., Quercus robur Ehrh. and Carpinus betulus L.) located in the State Forest of Champenoux in north-eastern France (48o75'N, 6o35' E, altitude 250 m). One hundred ectomycorrhizal root tips were sampled using forceps, cleaned of soil, organic matter, or litter fragments, and immediately frozen in liquid nitrogen. RNA extractions were carried out using the protocol described in the paper. A full-length cDNA library of L. quietus - Q. petraea ECMs was constructed from 1 ?g of total RNA using the SMART cDNA synthesis kit in lTriplEx2 (Clontech, Palo Alto, California). The resulting cDNA was packaged into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech). cDNA inserts bacterial clones derived from the cDNA library were amplified and inserts longer than 500 bp were single-pass sequenced from the 5' end using the FORNAT primer (5'-AAGCGCGCCATTGTGTTGGTACCC-3') using a CEQ 8000XL sequencer (Beckman-Coulter).
characteristics.characteristic: description
characteristics.text: ectomycorrhizas
characteristics.characteristic: organ
characteristics.text: Lactarius quietus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_111141
characteristics.characteristic: organism
characteristics.text: oak forest ectomycorrhizas
characteristics.characteristic: strain
characteristics.text: Lactarius quietus-Quercus petraea ectomycorrhizas from the State forest of Champenoux
characteristics.characteristic: title