characteristics.text: SAMN00153228
characteristics.characteristic: External Id
characteristics.text: LIBEST_021517
characteristics.characteristic: External Id
characteristics.text: Station Biologique de Roscoff
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:20.013Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suppression subtraction hybridisation (SSH) library corresponding to a differential screen between cDNA from immature sporophytes and immature gametophytes of the brown alga Ectocarpus siliculosus. Immature means that the algae had not yet produced reproductive structures. The SSH was carried out using the PCR-select kit (Clontech) following the recommended protocol except that an additional enrichment step (mirror orientation selection or MOS) was carried out (Rebrikov et al., 2000 Nucleic Acids Research 28: N20 e90). The cDNA fragments produced by the SSH protocol were cloned into the vector pCR2.1-TOPO.
characteristics.characteristic: description
characteristics.text: young gametophyte
characteristics.characteristic: dev-stage
characteristics.text: Ectocarpus siliculosus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2880
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Ec 32
characteristics.characteristic: strain
characteristics.text: Esil gametophyte SSH
characteristics.characteristic: title
characteristics.text: pCR2.1-TOPO
characteristics.characteristic: vector