characteristics.text: SAMN00166667
characteristics.characteristic: External Id
characteristics.text: LIBEST_023708
characteristics.characteristic: External Id
characteristics.text: NRC Plant Biotechnology Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:23.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: cultivar
characteristics.text: The library HLUPLC was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). HLUPLC was constructed using a cDNA library synthesis kit (Stratagene) and cDNAs ligated into the plasmid vector pBluescript SK+. The library was normalized using a hydroxyapatite-based method (C0t 2.5 and 5) (Bonaldo et al. (1996) Genome Res. 6, 791).
characteristics.characteristic: description
characteristics.text: Trichomes isolated from mid to ripe stage hop cones
characteristics.characteristic: dev-stage
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Trichome (lupulin gland)
characteristics.characteristic: tissue-type
characteristics.text: HLUPLC
characteristics.characteristic: title
characteristics.text: pBluescript SK+
characteristics.characteristic: vector