characteristics.text: SAMN00157126
characteristics.characteristic: External Id
characteristics.text: LIBEST_002567
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-11-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:10.930Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: T157
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from seed coats (100-200mgs) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complimentary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restrictions site. Sal I linkers adapers were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli Electromax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: seed coats of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1023
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector