characteristics.text: SAMN00156303
characteristics.characteristic: External Id
characteristics.text: LIBEST_001731
characteristics.characteristic: External Id
characteristics.text: National Institute of Diabetes and Digestive and Kidney Diseases
characteristics.characteristic: INSDC center name
characteristics.text: 1999-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:48.933Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Erythroid Cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000232
characteristics.characteristic: cell type
characteristics.text: Primary Culture of Peripheral Blood Mononuclear Cells
characteristics.characteristic: cell-line
characteristics.text: Human peripheral blood mononuclear cells were cultured in the presence or absence of erythropoietin. CD71++++ cells arising only in erythropoietin-supplemented medium were isolated by fluorescence activated cell sorting. Those cells demonstrated an average of six additional doublings in suspension culture and erythroid colony formation in methylcellulose. Suppression subtractive hybridization was used to construct the ad library (tester=sorted CD71++++ cells, driver=unsorted cells cultured without erythropoietin).
characteristics.characteristic: description
characteristics.text: Progenitor; EPO responsive CD71++++
characteristics.characteristic: dev-stage
characteristics.text: DH5alpha
characteristics.characteristic: lab-host
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Hembase; Erythroid Progenitor Cells (LCB:ad library)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII
characteristics.characteristic: vector