characteristics.text: LIBEST_009522
characteristics.characteristic: External Id
characteristics.text: SAMN00164035
characteristics.characteristic: External Id
characteristics.text: National Institute of Child Health and Human Development, NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2001-06-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:39.387Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA was reverse-transcribed to first strand cDNA using SuperScriptII reverse-transcriptase and tagged oligo-dT primer which contains several restriction sites including a NotI site: gactagttctagatcgcgatcgcgaGCGGCCGCccttttttttttttttt. Second strand DNA was synthesized by E. coli DNA polymerase I in combination with E. coli RNAse H and E. coli DNA ligase. Double stranded cDNA was ligated with SalI adapter. These cDNAs were cloned into the SalI/NotI site of pBluescript KS+ and transformed into E. coli Electromax DH10B by electroporation.
characteristics.characteristic: description
characteristics.text: bud to 10 somite stage embryos
characteristics.characteristic: dev-stage
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: NICHD_Zebrafish_normalized_I
characteristics.characteristic: title
characteristics.text: pBluescript KS+
characteristics.characteristic: vector