characteristics.text: SAMN00164929
characteristics.characteristic: External Id
characteristics.text: LIBEST_007231
characteristics.characteristic: External Id
characteristics.text: Sichuan University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-12-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:30.023Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sperm cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001005
characteristics.characteristic: cell type
characteristics.text: Guichao2
characteristics.characteristic: cultivar
characteristics.text: Tester cDNA was prepared from isolated rice sperm cells by PCR with the help of SMART PCR cDNA Synthesis Kit(Clontech) and driver cDNA was prepared from two-cell stage pollen of rice. Subtraction was carried out with the help of CLONTECH PCR-Select cDNA Subtraction kit. Subtracted cDNAs were inserted into pUCm-T plasmid.
characteristics.characteristic: description
characteristics.text: Isolated from mature pollen of rice
characteristics.characteristic: dev-stage
characteristics.text: E.coli JM109
characteristics.characteristic: lab-host
characteristics.text: Oryza sativa Indica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39946
characteristics.characteristic: organism
characteristics.text: Subtracted rice sperm cells cDNA Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUCm-T
characteristics.characteristic: vector