characteristics.text: SAMN00154716
characteristics.characteristic: External Id
characteristics.text: LIBEST_000100
characteristics.characteristic: External Id
characteristics.text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais
characteristics.characteristic: INSDC center name
characteristics.text: 1993-12-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:02.867Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total cellular RNA from male and female adult worms was extracted according to a modification (Puissant, C. and Houdebine, L. M. BioFeedback 8, 148-149, 1990) of the Guanidine Thiocyanate procedure (Chomczynski, P. and Sacchi, N. Anal. Biochem. 162, 156- 159, 1987). Poly (A)+ RNA was purified by oligo dT column and cDNA was synthesized as described previously (Adams, M. D. et al. Nature Genet. 4, 373-389, 1993). cDNA was ligated to a two fold molar excess of a NotI/HindIII digested plasmid DNA (lafmid BA vector, a phagemid derived from pEMBL, Adams, M. D. et al. Nature Genet. 4, 373-389, 1993)) and electroporated into E. coli strain DH10B (BRL). The library was amplified and further selected for clones containing long inserts (>500 bp) by purification of the plasmid DNA from a fragment of a 1% low-melting-point agarose gel, containing the smear of the library and electroporation into DH10B cells.
characteristics.characteristic: description
characteristics.text: DH10B, JM109
characteristics.characteristic: lab-host
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: HindIII
characteristics.characteristic: re_2
characteristics.text: NMRI
characteristics.characteristic: strain
characteristics.text: Schistosoma mansoni, adult worm, Gloria Franco
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: BA vector
characteristics.characteristic: vector