characteristics.text: SAMD00016619
characteristics.characteristic: External Id
characteristics.text: Genome Analysis Research Group, Extremophiles Research Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2010-12-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-11-12T03:29:47Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS000201
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS000201
characteristics.characteristic: SRA accession
characteristics.text: microbial mat metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_527640
characteristics.characteristic: organism
characteristics.text: This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine. As a first step of this project, we constructed a fosmid library from the DNA directly isolated from the microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we determined the paired-end sequence of all fosmid clones. We selected putative archaeal genome fragments from the metagenomic library. Total of 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. In addition, 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences.
characteristics.characteristic: sample comment
characteristics.text: DRS000201
characteristics.characteristic: sample name
characteristics.text: Composite genome of Caldiarchaeum subterraneus
characteristics.characteristic: title