characteristics.text: SAMN00157060
characteristics.characteristic: External Id
characteristics.text: LIBEST_002500
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 1999-10-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:08.973Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: genotype A17
characteristics.characteristic: cultivar
characteristics.text: cDNA was prepared from polyA+ enriched RNA from the mixture of cotyledons of five days old plants and leaves of two weeks old plants. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells.
characteristics.characteristic: description
characteristics.text: mixture of cotyledons from five days old plants and leaves obtained from two weeks old plants
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain SOLR
characteristics.characteristic: lab-host
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: leaves and cotyledons
characteristics.characteristic: tissue-type
characteristics.text: DSLC
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK +/-
characteristics.characteristic: vector