characteristics.text: SAMN00164196
characteristics.characteristic: External Id
characteristics.text: LIBEST_009689
characteristics.characteristic: External Id
characteristics.text: University of Washington
characteristics.characteristic: INSDC center name
characteristics.text: 2001-08-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:44.347Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ageratum conyzoides (Kanzaki et al, Biosci. Biotechnol. Biochem., 62 (12)2328-2333, 1998)
characteristics.characteristic: cell-line
characteristics.text: cDNA-AFLP technique. Ageratum conyzoides plant cells were collected 24 and 48 h after Agrobacterium tumefaciens- or mock- inoculation. RNA was extracted, mRNA was isolated, and double-stranded cDNA was synthesized. The cDNA was digested with TaqI and AseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing two additional bases at the 3' end.
characteristics.characteristic: description
characteristics.text: 24 and 48 h after either inoculation with Agrobacterium tumefaciens or mock-inoculation
characteristics.characteristic: dev-stage
characteristics.text: Ageratum conyzoides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_68299
characteristics.characteristic: organism
characteristics.text: AseI
characteristics.characteristic: re_1
characteristics.text: TaqI
characteristics.characteristic: re_2
characteristics.text: cell suspension culture
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000221
characteristics.characteristic: tissue-type
characteristics.text: Ac_At-1
characteristics.characteristic: title