characteristics.text: LIBEST_015744
characteristics.characteristic: External Id
characteristics.text: SAMN00154107
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2004-05-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:32.430Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA
characteristics.characteristic: cultivar
characteristics.text: tissue: Whole megagametophytes isolated from pine seeds, Whole embryos excised from these megagametophytes, whole somatic embryos and suspensor tissue from tissue culture, isolated from cell line A12. Pooled RNA from zygotic embryos, megagametophytes, and somatic embryos was used for library construction. Pine cones were harvested weekly from open-pollinated 7-56 mother trees, collections occurred from 7/01/02 until 10/15/02. Whole megagametophytes were first isolated from pine seeds, and whole embryos excised from these megagametophytes each was flash frozen. Embryo development was assessed using the system of Pullman et al (Pullman GS, Johnson S, Peter G, Cairney J, Xu N. 2003. Loblolly Pine somatic embryogenesis: development of a maturation medium and resulting embryo quality. Plant Cell Reports 21:747-758 (http://link.springer.de/link/service/journals/00299/contents/03/00586/). For photographs see Ciavatta et al 2001. (Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 (http://www.plantphysiol.org/cgi/content/full/127/4/1556)).
characteristics.characteristic: description
characteristics.text: E.coli DH10B-TonA
characteristics.characteristic: lab-host
characteristics.text: Zygotic Embryo and Megagametophyte, Somatic Embryo
characteristics.characteristic: organ
characteristics.text: Pinus taeda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3352
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRV
characteristics.characteristic: re_2
characteristics.text: Sequencing ESTs from loblolly pine embryos
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMV-SPORT 6.1
characteristics.characteristic: vector