characteristics.text: LIBEST_000944
characteristics.characteristic: External Id
characteristics.text: SAMN00155541
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1997-09-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:26.970Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Library was constructed in Stratagene Uni-Zap XR lambda vector with Zap-cDNA synthesis kit: EcoRI linkers were added to 5' ends and XhoI linkers were added to 3' ends and resulting cDNAs were ligated into Uni-Zap XR lambda vector. In vivo mass excision was used to generate cDNAs in the pBluescript SK vector. Initial phage library amplified from 900,000 independent phage, 99% recombinants (by X-gal staining), 90% inserts (by PCR amplification), 1.7 kb average insert (range 0.8 - 3.4 kb).
characteristics.characteristic: description
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: pooled 15-19 hour zebrafish embryos
characteristics.characteristic: tissue-type
characteristics.text: Appel Eisen zebrafish embryo 15 19hr
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector