characteristics.text: SAMN00153974
characteristics.characteristic: External Id
characteristics.text: LIBEST_012708
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 2003-02-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:04.323Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Jejunal Peyer's patches were isolated from healthy, 4-6 week old cross-bred pigs. RNA was extracted either immediately or after 3 hours stimulation in an Ussing chamber with one of the following treatments: 1) no treatment, 2) Salmonella choleraesuis vaccine strain SC-54, 3) phorbol myristate acetate, concanavalin A, and 8-bromo-cyclic AMP, and 4) lipopolysaccharide and cholera toxin. Each treatment was performed in the presence and absence of cycloheximide. Purified poly A + RNA from each of the treated tissues (2-4) was combined together, reverse transcribed, and cloned in to pCMVSPORT6 to make a library of approximately 530,000 recombinant clones with an average insert size of 1.0 kbp. Poly A+ RNA from freshly isolated and non-treated tissue from an Ussing chamber (treatment 1) was cloned in the same manner to produce an unstimulated cDNA library of approximately 900,000 clones with an average insert size of 1.5 kbp. Equal portions of the two libraries were pooled and then subtracted with porcine ST fibroblast RNA to create a subtracted porcine Peyer's patch library of approximately 6,000 clones with an average insert size of 1.0 kbp.
characteristics.characteristic: description
characteristics.text: small intestine, jejunum
characteristics.characteristic: organ
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Peyer's patch
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001211
characteristics.characteristic: tissue-type
characteristics.text: UMNMPM3
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSPORT6
characteristics.characteristic: vector