characteristics.text: SAMN00154121
characteristics.characteristic: External Id
characteristics.text: LIBEST_015935
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2004-06-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:38.127Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized from adult ovary and testis total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the ovary but not in the testis cDNA. The selectively amplified cDNA fragments (in average 400-800bp in length) were ligated into pT-Advantage (Clontech) transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.
characteristics.characteristic: description
characteristics.text: adult (fully mature)
characteristics.characteristic: dev-stage
characteristics.text: pooled ovary
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Toh (Singaporean strain)
characteristics.characteristic: strain
characteristics.text: adult ovary subtracted (TLL)
characteristics.characteristic: title
characteristics.text: pT-Advantage
characteristics.characteristic: vector