characteristics.text: LIBEST_023085
characteristics.characteristic: External Id
characteristics.text: SAMN00165654
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:04.267Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: RNA was pooled from C. zeae-maydis growing on 0.2x PDA 3 and 5 days after inoculation and from cultures grown for 5 days on V8-agar and 0.2x PDA supplemented with 10 mM ammonium phosphate. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: 3-10 day old plate cultures
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Cercospora zeae-maydis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_135779
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: SCOH1-5
characteristics.characteristic: strain
characteristics.text: whole mycelia
characteristics.characteristic: tissue-type
characteristics.text: CBYB Cercospora zeae-maydis cercosporin biosynthesis large-insert
characteristics.characteristic: title
characteristics.text: pCMV-SPORT6
characteristics.characteristic: vector