characteristics.text: LIBEST_014170
characteristics.characteristic: External Id
characteristics.text: SAMN00166071
characteristics.characteristic: External Id
characteristics.text: Michigan State University
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:47.573Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: INRA Clone 717
characteristics.characteristic: cultivar
characteristics.text: The Stem of one month-old Populus tremula x Populus alba trees was divided into sections according to the developmental stage, starting from the shoot apex until the base of the stem. The lower stem was further divided into bark and xylem by pealing of the bark. Totally we obtained six samples: three stem sections with increasing xylem production, bark, xylem and leaf with its main vein removed with a scalpel. Poly A+ RNA was extracted from those samples and subjected to cDNA-AFLP profiling. Constitutively expressed fragments or leaf up-regulated fragments were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was purified and sequenced directly with one of the PCR primers.
characteristics.characteristic: description
characteristics.text: Stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: organ
characteristics.text: Populus tremula x Populus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80863
characteristics.characteristic: organism
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Hybrid aspen various expression patterns
characteristics.characteristic: title