characteristics.text: SAMN00161337
characteristics.characteristic: External Id
characteristics.text: LIBEST_006802
characteristics.characteristic: External Id
characteristics.text: Brigham and Women's Hospital, Harvard Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:16.940Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mast cell (in vitro differentiated)
characteristics.characteristic: cell type
characteristics.text: Mouse bone marrow-derived mast cells were developed by culturing progentiors for 3-6 weeks in 50% RPMI-1640 medium supplemented with 100 ng/ml c-kit ligand and 50% WEHI-3 cell-conditioned medium (as a source of interleukin 3). The first-strand cDNA was synthesized from mRNA using NotT priming (Invitrogen). The resulting cDNAs were ligated with an EcoRI/BstXI adaptor and then cloned into pcDNA3 mammalian expression vector by Invitrogen.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Bxt XI
characteristics.characteristic: re_2
characteristics.text: BALB/cJ
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000602
characteristics.characteristic: strain
characteristics.text: starting cells - non-fractionated bone marrow cells
characteristics.characteristic: tissue-type
characteristics.text: Mouse Bone Marrow-derived Mast Cell Expression Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pcDNA3 (Invitrogen)
characteristics.characteristic: vector