characteristics.text: LIBEST_014702
characteristics.characteristic: External Id
characteristics.text: SAMN00154052
characteristics.characteristic: External Id
characteristics.text: Concordia University, Department of Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2003-12-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:02.540Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Crown (50%) and leaf (50%) tissues from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20Cs from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20, wheat plants were transferred to 4C in the light. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. The last 6 populations: After 7 days of growth at 20C, wheat plants were transferred to 4C in the dark. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI. In addition, this library used a primer for second strand synthesis that annealed to an artificial sequence (RNA oligo) added before first strand synthesis. Therefore when sequences from EST generated from this library will be masked for vector and adaptor sequences, an additional masking step will have to be included to mask this RNA oligo that is common to all clones (sequence CGACTGGAGCACGAGGACACTGACATGGACTGAAGGAGTAGAAA).
characteristics.characteristic: description
characteristics.text: Crown and leaf
characteristics.characteristic: organ
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: Triticum aestivum FGAS: Library 6 CAP GATE 1
characteristics.characteristic: title
characteristics.text: pCMV.SPORT6
characteristics.characteristic: vector