characteristics.text: SAMN00154879
characteristics.characteristic: External Id
characteristics.text: LIBEST_000265
characteristics.characteristic: External Id
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:07.277Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ds-cDNA was prepared from oligo-dT selected mRNA by priming with a XhoI oligo- dT oligomer and then adding the second strand to RNase-nicked DNA:RNA hybrid with DNA PolI. EcoRI adaptors were added to the ends, the ds-cDNAs were then digested with EcoRI- XhoI and size-selected. These were directionally-cloned into the ZAP phage vector, excised as plasmids, and then individually analyzed.
characteristics.characteristic: description
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: W64A2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007619
characteristics.characteristic: strain
characteristics.text: vegetative meristem
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Zap
characteristics.characteristic: vector