characteristics.text: SAMN00155193
characteristics.characteristic: External Id
characteristics.text: LIBEST_000590
characteristics.characteristic: External Id
characteristics.text: Harvard Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 1997-01-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:15.043Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: No library was made. cDNA from individual Leishmania major lines containing random transposon insertions that activated a selectable mariner element (MosHYG) was amplified by PCR. PCR primers corresponded to the mini-exon sequence present in the 5' end of every trypanosomatid mRNA (forward primer) and to MosHYG sequences (reverse primer). PCR products were cloned in the pGEM-T vector and sequenced using SP6/T7 primers.
characteristics.characteristic: description
characteristics.text: promastigote
characteristics.characteristic: dev-stage
characteristics.text: Leishmania major
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5664
characteristics.characteristic: organism
characteristics.text: LT252
characteristics.characteristic: strain
characteristics.text: Random insertions of transposon MosHYG in Leishmania major
characteristics.characteristic: title