characteristics.text: SAMN00165358
characteristics.characteristic: External Id
characteristics.text: LIBEST_022813
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:55.617Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Kanlow
characteristics.characteristic: cultivar
characteristics.text: In Vivo Conditions: dark grown at 28C. Library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. Library is non-amplified. Tissue was provided by C. Tobias (USDA ARS, Albany, CA), library was constructed in the laboratory of P. Twigg (University of Nebraska, Kearney) and sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: embryogenic and non-embrogenic
characteristics.characteristic: dev-stage
characteristics.text: DH5alpha FT
characteristics.characteristic: lab-host
characteristics.text: callus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0005052
characteristics.characteristic: organ
characteristics.text: Panicum virgatum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_38727
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: CBYX Panicum virgatum Kanlow callus
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector