characteristics.text: LIBEST_020980
characteristics.characteristic: External Id
characteristics.text: SAMN00152735
characteristics.characteristic: External Id
characteristics.text: Northwest A & F University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: young leaves
characteristics.characteristic: cell type
characteristics.text: Total RNA was extracted by Trizol, then reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech company) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.
characteristics.characteristic: description
characteristics.text: stage at 4-5 leaves, after driver was inoculated Stripe rust pathogen CY32 in 2 days
characteristics.characteristic: dev-stage
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: SSH-cDNA Library of normal leaves of stripe rust resistant material Shaanmai 139 compared with stripe rust infection
characteristics.characteristic: title