characteristics.text: LIBEST_004039
characteristics.characteristic: External Id
characteristics.text: SAMN00158592
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:49.190Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mesenchymal/Epithelial
characteristics.characteristic: cell type
characteristics.text: Restriction Enzymes 1; ApaI, AatII, SphI, NcoI, BstZI, NotI, SacII, and EcoRI SpeI, EcoRI, NotI, BstZI, PstI, SalI, NdeI, SacI, BstXI, and NsiI cDNA fragment PCR-amplified in mRNA differential display analysis; cloned in pGEM-TEasy (Promega); its expression is developmentally regulated during mesenchymal-epithelial conversion in the metanephric kidney.
characteristics.characteristic: description
characteristics.text: 13 dpc-16dpc
characteristics.characteristic: dev-stage
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Metanephric mesenchyme
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003220
characteristics.characteristic: tissue-type
characteristics.text: Explanted metanephric mesenchyme induced to differentiate into epithelial structures of the nephron ex vivo.
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-TEasy (Promega).
characteristics.characteristic: vector