characteristics.text: LIBEST_020954
characteristics.characteristic: External Id
characteristics.text: SAMN00166634
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.003Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was made from dT primed cDNA and cloned into pCS107 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCS107 vector.
characteristics.characteristic: description
characteristics.text: Stage 25
characteristics.characteristic: dev-stage
characteristics.text: ElectroMAX DH10B T1 Phage Resistant cells
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: PopA
characteristics.characteristic: strain
characteristics.text: whole
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C25326
characteristics.characteristic: tissue-type
characteristics.text: NICHD_XGC_trop_25
characteristics.characteristic: title
characteristics.text: pCS107
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002843
characteristics.characteristic: vector