characteristics.text: SAMN00155450
characteristics.characteristic: External Id
characteristics.text: LIBEST_000851
characteristics.characteristic: External Id
characteristics.text: San Francisco General Hospital-University of California, San Francisco
characteristics.characteristic: INSDC center name
characteristics.text: 1997-04-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:23.170Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The C. parvum cDNA library was prepared by Drs. Norman J. Pieniazek, Michael J. Arrowood, Susan B. Slemenda, and Jan R. Mead at the Centers for Disease Control and Prevention (Atlanta, Georgia). Poly A+ RNA was separated from total C. parvum RNA using the Poly(A) Quik mRNA Isolation Kit from Stratagene. Directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I linkers, and digestion with Xho I, all using the Stratagene ZAP-cDNA synthesis kit. The cDNA was cloned into the EcoR I and Xho I sites of Lambda Uni-ZAP XR vector; the unamplified library was >95% recombinant and contained 3.8 X 10(6) independent clones. PCR analysis of 20 random clones indicated that the average insert size was ca. 1.1 kb.
characteristics.characteristic: description
characteristics.text: sporozoite
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1 Blue MRF' Kan
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: IOWA
characteristics.characteristic: strain
characteristics.text: uniZAPCpIOWAsporoLib3
characteristics.characteristic: title
characteristics.text: UniZAP XR
characteristics.characteristic: vector