characteristics.text: SAMN00002777
characteristics.characteristic: External Id
characteristics.text: CNRS
characteristics.characteristic: INSDC center name
characteristics.text: 2009-10-19T09:09:40.377Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:49:47.067Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS004336
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS004336
characteristics.characteristic: SRA accession
characteristics.text: RNA from three pooled preparations of exosome-like vesicles prepared by differential centrifugation from the supernatant of MonoMac-6 cells. Exosome-like vesicles were resuspended in 250 uL PBS and isolated with Trizol LS. A library of 19-33 nucleotide RNA was prepared according to the methods of Pfeffer S, Lagos-Quintana M, Tuschl T. Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.4. Cloning of Small RNA Molecules. RNA isolation, library preparation and 454 technology sequencing was performed in parallel with the library for total cell RNA. The adaptors used for library preparation were as follows: 5' (454 A primer - identifying tag - 5' adaptor; GCCTCCCTCGCGCCATCAG-GATC-GAATTCCTCACTAAA-cloned RNA) and 3'(cloned RNA - 3'adaptor - 454 B primer; TTTAACCGCGAATTC-CTGAGCGGGCTGGCAAGGC).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Generic sample from Homo sapiens
characteristics.characteristic: title