characteristics.text: SAMN00164480
characteristics.characteristic: External Id
characteristics.text: LIBEST_009980
characteristics.characteristic: External Id
characteristics.text: National Institute on Aging/National Institutes of Health
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:51.670Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 24.9 microgram of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127:1737-1749 (2000) [PMID: 10725249]), purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were digested with SalI and NotI enzymes, and cloned into SalI and NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with ligation mixture by the chemical method. The average insert size is about 1.8 kb. The library was constructed by Yulan Piao (NIA).
characteristics.characteristic: description
characteristics.text: Newborn
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001372
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: heart
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000948
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Newborn Heart
characteristics.characteristic: tissue-type
characteristics.text: NIA Mouse Newborn Heart cDNA Library
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pSPORT1 (Invitrogen)
characteristics.characteristic: vector