characteristics.text: LIBEST_016774
characteristics.characteristic: External Id
characteristics.text: SAMN00164992
characteristics.characteristic: External Id
characteristics.text: University of Arkansas
characteristics.characteristic: INSDC center name
characteristics.text: 2004-12-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:02.793Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PI560247
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_90524
characteristics.characteristic: cultivar
characteristics.text: This sequence was obtained by cDNA-AFLP where cDNAs were synthesized from reverse transcription of mRNA isolated from seeds at different stages of germination and seedlings at early phase of growth under chilling stress (13C/10C). cDNA was digested with EcoRI and MseI restriction endonucleases simultaneously. Subsequently, the cDNA fragments were ligated to EcoRI and MseI adapters to generate template DNA for amplification. These common adapter sequences flanking variable cDNA sequences serve as the primer binding sites on these restriction fragments. Small DNA fragments were generated, then amplified by PCR using different primer combinations including two and three selective bases in the presence of 33P for radioactive labeling of the amplified fragments. The PCR products of the cold-treated and control cDNAs were run on a denaturing polyacrylamide gel. The up-regulated bands were isolated from the gel, cloned in pCR2.1 vector (Invitrogen) and sequenced in one direction using the T3 primer.
characteristics.characteristic: description
characteristics.text: Coleoptile and radicle stages
characteristics.characteristic: dev-stage
characteristics.text: DH5alpha
characteristics.characteristic: lab-host
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Oryza sativa Japonica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: MseI
characteristics.characteristic: re_2
characteristics.text: Japonica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: strain
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Rice cold stress germination cDNA-AFLP
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector