characteristics.text: LIBEST_021586
characteristics.characteristic: External Id
characteristics.text: SAMN00153293
characteristics.characteristic: External Id
characteristics.text: J. Craig Venter Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:18:14.220Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones. ~isolation_source: border of a cereal field~ ~country: Spain: Colmenar Viejo, just N of Madrid~ ~lat-lon: 40.6 N, 3.7 W~
characteristics.characteristic: description
characteristics.text: Raphanus raphanistrum subsp. raphanistrum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_109997
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: whole seedling (with 1 set of true leaves), buds, and anthers
characteristics.characteristic: tissue-type
characteristics.text: RR2(MS)
characteristics.characteristic: title
characteristics.text: pDNR-LIB(Clontech)
characteristics.characteristic: vector