characteristics.text: LIBEST_017271
characteristics.characteristic: External Id
characteristics.text: SAMN00154203
characteristics.characteristic: External Id
characteristics.text: Oklahoma State University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:19.767Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA suppression subtraction library from oropharyngeal tonsils and mandibular lymph nodes of diseased and healthy European wild boar naturally exposed to M. bovis. Total RNA was isolated using TriReagent (Sigma, St. Louis, MO, USA) according to manufacturer's instructions. After diagnosis of diseased and healthy individuals by pathology, culture and spoligotyping of mycobacteria, pools of 20 ug RNA were made for healthy (N=2; 10 ug RNA/each) and diseased (N=4; 5 ug RNA/each) animals. SSH was performed at Evrogen JCS (Moscow, Russia) as previously described (Diachenko et al., 1996; 1999). Tester and driver RNAs were subtracted in both directions to construct 4 SSH libraries containing differentially expressed cDNAs in diseased (reverse-subtracted) and healthy (forward-subtracted) samples for oropharyngeal tonsils and mandibular lymph nodes. Approximately 100 clones from each library were randomly picked up and subjected to differential hybridization with subtracted and non-subtracted probes using the PCR-select differential screening kit (Clontech, Palo Alto, CA, USA), which resulted in >90% differentially expressed cDNAs.
characteristics.characteristic: description
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: lab-host
characteristics.text: oropharyngeal tonsils and mandibular lymph nodes
characteristics.characteristic: organ
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: oropharyngeal tonsils and mandibular lymph nodes of diseased and healthy European wild boar
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAL9
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector