characteristics.text: LIBEST_022982
characteristics.characteristic: External Id
characteristics.text: SAMN00165527
characteristics.characteristic: External Id
characteristics.text: USDA-ARS FRRL
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:00.863Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 5'during cDNA synthesis: ACGGCCGGG-insert (for Elymus lanceolatus clones) or ACGGCCGAGGG-insert (for Elymus wawawaiensis clones).
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Elymus wawawaiensis/Elymus lanceolatus mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_519048
characteristics.characteristic: organism
characteristics.text: EcoRI (5' side of insert)
characteristics.characteristic: re_1
characteristics.text: NotI (3' side of insert)
characteristics.characteristic: re_2
characteristics.text: Subterranean tiller (Elymus wawawaiensis ) or tiller/rhizome (Elymus lanceolatus) meristems
characteristics.characteristic: tissue-type
characteristics.text: BG03- primary and normalized libraries
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBS II SK(+)
characteristics.characteristic: vector