characteristics.text: SAMN00156884
characteristics.characteristic: External Id
characteristics.text: LIBEST_002321
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-08-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:04.290Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from nodules on the roots of 2.5 month-old Glycine max 'Williams' plants that were greenhouse grown. The The cDNA library was prepared using the Stratagene pBluescript II SK(+) library construction kit. First strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (v=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA polymerase and size- fractionated with a 400 bp cutoff, using a SizeSep 400 Spun column from Pharmacia. The column eluent was ligated to EcoRI adaptors and phosphporylated. The XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size- fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Predigested vector (pBluescript II SK+) that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=56) and sequence (n=16). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.
characteristics.characteristic: description
characteristics.text: XL10-Gold
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: root nodules of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1020
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector