characteristics.text: LIBEST_021298
characteristics.characteristic: External Id
characteristics.text: SAMN00166635
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:14.860Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly(A)+RNA was isolated from 58 ug of total RNA by one round of oligo(dT) selection with oligo(dT) coated magnetic particles (Seradyn, Inc.). From 1.2 ug of this poly(A)+RNA mRNA, a cDNA library was constructed by using an oligo dT primer-adapter containing a Not I site and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA. After the second strand was synthesized, the double stranded cDNA was digested with Not I, size fractionated (>1.4 kb) and cloned directionally into the Not I and Sma I sites of the pCS111 vector. From one bulk ligation (300 ng of pCS111 vector, Not I-Sma I cut, and 120 ng of Not I digested cDNA per 120 ul of ligation), followed by electroporation into T1 phage resistant E. coli, 1.2 x 107 primary clones were produced. The cDNA inserts from 24 randomly picked clones were sized by digestion with Not I and Eco RI. The average insert size of these 24 clones was 1.9 kb.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B T1 Phage-resistant
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SmaI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Eye
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000970
characteristics.characteristic: tissue-type
characteristics.text: NICHD_XGC_tropEye1
characteristics.characteristic: title
characteristics.text: pCS111
characteristics.characteristic: vector