characteristics.text: LIBEST_023754
characteristics.characteristic: External Id
characteristics.text: SAMN00166713
characteristics.characteristic: External Id
characteristics.text: National Institute of Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-06-04T21:03:09.445Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Gametophores were cultivated on sterile peat pellets (Jiffy-7; Jiffy Products International AS, Kristansand, Norway) for 1 to 1.5 months at 25C under continuous light. The plants were moved to 15C under 8-h light/16-h dark conditions to induce gametangia and cultivated for 11 to 26 days. Shoot tips with archegonia and antheridia were collected. Shoot tips with archegonia that were brown in color, which is an indicator of fertilization, were removed. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library constructed by Yutaka Suzuki and Sumio Sugano (University of Tokyo, Institute of Medical Science). synonym: Physcomitrella patens subsp. patens
characteristics.characteristic: description
characteristics.text: Physcomitrium patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: DraIII
characteristics.characteristic: re_1
characteristics.text: DraIII
characteristics.characteristic: re_2
characteristics.text: Gransden
characteristics.characteristic: strain
characteristics.text: gametangia (shoot tip with antheridia and archegonia)
characteristics.characteristic: tissue-type
characteristics.text: ppaa full-length cDNA library; antheridia and archgonia
characteristics.characteristic: title
characteristics.text: pME18S-FL3
characteristics.characteristic: vector