characteristics.text: LIBEST_006831
characteristics.characteristic: External Id
characteristics.text: SAMN00161366
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:18.183Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.5 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
characteristics.characteristic: description
characteristics.text: DH10B (T1 phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: prostate
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002367
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: SfiI (ggccgcctcggcc)
characteristics.characteristic: re_1
characteristics.text: SfiI (ggccattatggcc)
characteristics.characteristic: re_2
characteristics.text: adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000228
characteristics.characteristic: tissue-type
characteristics.text: NIH_MGC_60
characteristics.characteristic: title
characteristics.text: plasmid (chloramphenicol resistant, grown at 30C)
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB (Clontech)
characteristics.characteristic: vector