characteristics.text: LIBEST_021733
characteristics.characteristic: External Id
characteristics.text: SAMN00153396
characteristics.characteristic: External Id
characteristics.text: University of Colorado Health Sciences Center
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:25.900Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Differential display of mRNA technique was used to detect host gene expression changes during the drought stress induction. mRNA extracted from induced and not induced (control) 21 days soybean roots. To induce drought stress, roots was kept in dark without solution for 50, 100 and 150 min. Seventy-four differential display fragments were obtained by using DDRT-PCR. After RT-PCR analyses, three positive clones were eventually obtained. The DDRT-PCR fragments were cloned in the pGEM-T vector (Easy Vector System I, Promega) and sequenced.
characteristics.characteristic: description
characteristics.text: day 21 post-germination
characteristics.characteristic: dev-stage
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: tissue-type
characteristics.text: cDNA sequences from soybean roots induced by drought stress
characteristics.characteristic: title