characteristics.text: LIBEST_023619
characteristics.characteristic: External Id
characteristics.text: SAMN00166512
characteristics.characteristic: External Id
characteristics.text: Austrian Research Centers GmbH - ARC
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:20.477Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Yungay
characteristics.characteristic: cultivar
characteristics.text: Potato cv. Yungay (Peru) cDNA library targeting genes expressed in leaves during interaction with the potato pathogen Phytophthora infestans (a virulent isolate). Adult plants prior to blooming were spray-inoculated, leaves were harvested and immersed immediately in liquid nitrogen, at 4 dpi when first disease symptoms were visible. This library is part of the RLP microarray at the Trognitz lab. Normalization was achieved by one cycle of melting and re-association of the cDNA, followed by separation of the remaining ss-cDNA (normalized cDNA) from the re-associated ds-cDNA by passing through hydroxylapatite column chromatography. The ss-cDNA was amplified in 11 LA-PCR cycles. For directional cloning, cDNA was subjected to partial exonuclease treatment to generate 5'-Eco RI overhangs and then digested with Not I. Size fractionation of Not I treated cDNAs >0.5 kb was done on agarose gels. The cDNA was directionally ligated into vector. Ligations were electroporated into T1 phage-resistant TransforMaxTM EC100TM-T1 (Epicentre) electro-competent cells.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Solanum tuberosum subsp. andigenum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80418
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: Solanum tuberosum cv. Yungay adult leaf, normalized
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBS II sk+
characteristics.characteristic: vector