characteristics.text: SAMN00154993
characteristics.characteristic: External Id
characteristics.text: LIBEST_000381
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1995-12-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.913Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified from 4 x 10E5 filariform larvae which had been isolated from infected humans. cDNA was constructed and, using adaptors, was cloned unidirectionally into the vector from the EcoRI site to the XhoI site. The library has an unamplified titer of 1.5 x 10E6 pfu/ml and an amplified, undiluted titer of 7 x 10E9 pfu/ml. The average insert size of the unamplifed library is 975 bp (range, 500-1500).
characteristics.characteristic: description
characteristics.text: XL-1 Blue MRF' (Stratagene)
characteristics.characteristic: lab-host
characteristics.text: Strongyloides stercoralis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6248
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Filariform larvae obtained from humans
characteristics.characteristic: strain
characteristics.text: TBN95TM-SSFH
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR (Stratagene)
characteristics.characteristic: vector