characteristics.text: SAMN00007414
characteristics.characteristic: External Id
characteristics.text: Max-Planck-Institut for molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2010-01-20T04:26:22.333Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:52:43.580Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS009351
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS009351
characteristics.characteristic: SRA accession
characteristics.text: Micrococcal nuclease (MNase) is frequently used to determine nucleosome positions both in vitro and in vivo. This is achieved by digesting chromatin by MNase and selecting nucleosomal sized DNA fragments (~150 base pairs). To check to what extend the resulting data is biased by the use MNase and the size selection, a control experiment was performed, where naked yeast DNA was digested with MNase and fragments of 150 base pairs were selected. The MNase data shows a good correspondence to the data obtained by digesting chromatin assembled in vitro as well as chromatin isolated in vivo.
characteristics.characteristic: description
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: The effect of MNase on nucleosome positioning data
characteristics.characteristic: title