characteristics.text: SAMN00155001
characteristics.characteristic: External Id
characteristics.text: LIBEST_000389
characteristics.characteristic: External Id
characteristics.text: Anna University
characteristics.characteristic: INSDC center name
characteristics.text: 1995-12-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:10.060Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA library of the B.malayi L4 stage was constructed at Johns Hopkins University. The presence of a conserved 22 nucleotide splice leader at 5'end of large proportion of mature messages was exploited in the linked Reverse transcriptase-polymerase chain reaction in the construction of this librry. The library had unamplified phage titre of 6.4X10E5 pfu per ml and 94% of the clones have inserts (mean length approx 700 bp). Acknowledgements: Nithyakalyani Raghavan, Inca Ghosh, Ward Eisinger, Alan Scott Dept. of Molecular Microbiology and Immunology School of Public health and Hygiene, Johns Hopkins University, 615, N.Wolfe street,Baltimore MD 21205, USA. Tel 410 955 3442 Fax: 410 955 0105
characteristics.characteristic: description
characteristics.text: XL-1 Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Xba I
characteristics.characteristic: re_1
characteristics.text: EcoR I
characteristics.characteristic: re_2
characteristics.text: JHU93SL-BmL4 (JHU)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Uni-ZAP XR
characteristics.characteristic: vector