characteristics.text: SAMN00153934
characteristics.characteristic: External Id
characteristics.text: LIBEST_012444
characteristics.characteristic: External Id
characteristics.text: UMR INRA/Universite Henri Poincare 1136
characteristics.characteristic: INSDC center name
characteristics.text: 2003-06-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:58.157Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: S238N
characteristics.characteristic: cultivar
characteristics.text: Laccaria bicolor Maire Orton; cDNA library from mycelium of Laccaria bicolor grown for three weeks on agar-plates containing high sugar (20 g/l glucose, 5 g/l maltose) Pachlewski medium. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).
characteristics.characteristic: description
characteristics.text: three-weeks-old
characteristics.characteristic: dev-stage
characteristics.text: free-living vegetative mycelium
characteristics.characteristic: organ
characteristics.text: Laccaria bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29883
characteristics.characteristic: organism
characteristics.text: mycelium of Laccaria bicolor grown for three weeks
characteristics.characteristic: title
characteristics.text: pTriplEx2
characteristics.characteristic: vector