characteristics.text: SAMN00150657
characteristics.characteristic: External Id
characteristics.text: LIBEST_020075
characteristics.characteristic: External Id
characteristics.text: Centro de Investigacion y de Estudios Avanzados del IPN
characteristics.characteristic: INSDC center name
characteristics.text: 2007-01-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:37.137Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Pinto Villa
characteristics.characteristic: cultivar
characteristics.text: Plants of Phaseolus vulgaris cv. Pinto Villa were grown under greenhouse conditions for 30 days after emerging. Relative water content in soil (sRWC) and in leaf (lRWC) was registered as a measure of water status by using the next formula (Bernacchia, Salamini and Bartels, 1996): RWC (%) = [(Fresh wt - Dry wt) / (Turgid wt - Dry wt)] x 100. It was considered lRWC between 50-70%% as intermediate drought stress. Water stress was developed by withholding irrigation from 3 to 8 days representing various dehydration levels. Suppression subtractive hybridization (SSH) was performed by using the PCR-select cDNA subtraction kit (Clontech, USA) according to manufacturer's instructions. The contrasting samples were selected according to RWC data. The driver, or experimental sample, contained mRNA isolated from leaves under intermediate drought conditions. The tester sample, or control sample, contained mRNA isolated from leaves of plants under control conditions.
characteristics.characteristic: description
characteristics.text: 30 day old plants
characteristics.characteristic: dev-stage
characteristics.text: E. coli TOP 10F
characteristics.characteristic: lab-host
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Phaseolus vulgaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3885
characteristics.characteristic: organism
characteristics.text: RsaI
characteristics.characteristic: re_1
characteristics.text: RsaI
characteristics.characteristic: re_2
characteristics.text: Phaseolus vulgaris leaves under intermediate drought stress
characteristics.characteristic: title
characteristics.text: pDrive
characteristics.characteristic: vector