characteristics.text: LIBEST_016040
characteristics.characteristic: External Id
characteristics.text: SAMN00152695
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2004-07-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:40.587Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA obtained from 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.9 kb. This is a primary library (normalized library is NIH_MGC_251) and was constructed by Open Biosystems. Note: this is a NIH_MGC library
characteristics.characteristic: description
characteristics.text: DH10B TonA
characteristics.characteristic: lab-host
characteristics.text: thymus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002370
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: EcoRV
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: NIH_MGC_250
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pExpress-1
characteristics.characteristic: vector