characteristics.text: LIBEST_021861
characteristics.characteristic: External Id
characteristics.text: SAMN00154510
characteristics.characteristic: External Id
characteristics.text: National University of Singapore
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:29.407Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA and mRNA were isolated and purified following the protocols of the RNeasy Maxi Kit and the Oligotex mRNA Mini Kit, respectively (QIAGEN). The cDNA library was constructed following the protocols of the ZAP-cDNA Synthesis Kit (Stratagene). Plasmids were isolated and inserts end-sequenced by T3 promoter primer. Traces were processed for submission to dbEST.
characteristics.characteristic: description
characteristics.text: XLI-blue
characteristics.characteristic: lab-host
characteristics.text: Whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Dermatophagoides pteronyssinus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6956
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: Male and female
characteristics.characteristic: sex
characteristics.text: Dermatophagoides pteronyssinus cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescriptSK(-)(Stratagene)
characteristics.characteristic: vector