characteristics.text: SAMN00166583
characteristics.characteristic: External Id
characteristics.text: LIBEST_015539
characteristics.characteristic: External Id
characteristics.text: CNRS UMR 8080
characteristics.characteristic: INSDC center name
characteristics.text: 2004-03-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-08-29T21:03:14.856Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Xenopus tropicalis polyA+ RNA was obtained from brain and spinal cord of tadpoles at stages 58-61 and 62-64. cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xtbs, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Dr. L. Coen and Prof. B. Demeneix (Museum National d'Histoire Naturelle and CNRS UMR 5166, Paris, France).
characteristics.characteristic: description
characteristics.text: metamorphosis stage 58-64
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Ivory Coast
characteristics.characteristic: strain
characteristics.text: pool of brains and spinal cords from tadpoles
characteristics.characteristic: tissue-type
characteristics.text: Xenopus tropicalis xtbs plasmid library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSPORT6_xtbs
characteristics.characteristic: vector