characteristics.text: SAMN00155826
characteristics.characteristic: External Id
characteristics.text: LIBEST_001240
characteristics.characteristic: External Id
characteristics.text: Smith College Department of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1998-04-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:35.503Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Lymphatic filarial nematode parasite of humans. cDNA was amplified by PCR using T3 and T7 primers from conventionally constructed libraries: L3 (SAW94WL-BmL3), microfilaria (SAW94LS-BmMf), adult male (SAW94NL-BmAm) and adult female (SAW96MLW-BmAf). Microfilaria, adult male and adult female cDNAs were subtracted from L3 cDNA using the PCR-select cDNA subtraction kit (Clontech). The library has 2.6 x 106 independent recombinants and the average insert size is ~500bp. The library was constructed by Lori Saunders. The library is available from Dr. S. A. Williams, email: genome@smith.edu
characteristics.characteristic: description
characteristics.text: L3 cDNA subtracted with microfilaria, adult male and adult female cDNA libraries
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Brugia malayi L3 subtracted cDNA library (SAW97LS-BmL3SA)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP Express
characteristics.characteristic: vector