characteristics.text: SAMN00164580
characteristics.characteristic: External Id
characteristics.text: LIBEST_022345
characteristics.characteristic: External Id
characteristics.text: Massachusetts Institute Of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:41.900Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Whole animal
characteristics.characteristic: cell type
characteristics.text: ceh-30 (C33D12.7) PCR product from Shai Shaham's C. elegans cDNA library. PCR was performed using a gene-specific primer whose sequence is given in the comments of the EST sequence file and a primer specific to the vector backbone. Products were reamplified using a second gene-specific primer whose sequence is given in the comments of the EST sequence file and the same primer specific to the library vector. Products were ligated into pGEM T-Easy. Individual transformants were picked, miniprepped, and sequenced using SP6 and T7 primers. This sequence file has been trimmed to remove primer and vector sequences.
characteristics.characteristic: description
characteristics.text: Whole animal
characteristics.characteristic: organ
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: N2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004028
characteristics.characteristic: strain
characteristics.text: Whole animal
characteristics.characteristic: tissue-type
characteristics.text: Shai Shaham's C. elegans cDNA library
characteristics.characteristic: title