characteristics.text: SAMN00157335
characteristics.characteristic: External Id
characteristics.text: LIBEST_002776
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-12-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:16.380Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/NotI sites using the following 5' adaptor: gaattccccggg and 3' adaptor: agagaactagtgtcgacgcggccgcttttttttttttttttttt. Vector designed for mRNA injections (reference Lemaire et al. (1995) 81:85-94). Average insert size 1.6kb. Primary library, non-amplified. Library constructed by N. Pollet, PhD REF: Glinka, et al. (1996) Combinatorial signaling by Xwnt-11 and Xnr3 in the organizer epithelium. Mech. Dev. 60:221-231. Note: This is a Xenopus Gene Collection (XGC) library.
characteristics.characteristic: description
characteristics.text: stage 13
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007106
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue, F+
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: whole embryo, hyperdorsalized by LiCl treatment
characteristics.characteristic: tissue-type
characteristics.text: Xenla 13LiCl
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBSRN3 (modified pBluescript KS+, see description below)
characteristics.characteristic: vector