characteristics.text: LIBEST_005334
characteristics.characteristic: External Id
characteristics.text: SAMN00159874
characteristics.characteristic: External Id
characteristics.text: Wageningen University and Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2000-06-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:26.493Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized using SMART III oligo, CDS III oligo d(T)30 (Clontech) and Superscript II reverse transcriptase (Life Technology). cDNA clones were size fractionated and directionally ligated in the Sfi IA restriction site at 5' end and Sfi IB at 3' end of pMAK1. pMAK1 was derived from the plasmid pcDNA II (Invitrogen).
characteristics.characteristic: description
characteristics.text: second stage preparasitic juveniles (J2)
characteristics.characteristic: dev-stage
characteristics.text: Meloidogyne incognita
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6306
characteristics.characteristic: organism
characteristics.text: Sfi IA
characteristics.characteristic: re_1
characteristics.text: Sfi IB
characteristics.characteristic: re_2
characteristics.text: Meloidogyne incognita J2 (#MD99-1)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMAK1
characteristics.characteristic: vector