characteristics.text: LIBEST_007130
characteristics.characteristic: External Id
characteristics.text: SAMN00161663
characteristics.characteristic: External Id
characteristics.text: University of Florida
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:27.057Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from asynchronous blood stage forms of the cloned ANKA isolate of P. berghei grown in laboratory Swiss white mice. Contaminating host white cells had previously been removed using a novel biomagnetic bead protocol (J. Carlton et al., manuscript in preparation). PolyA+ RNA was extracted and reverse transcribed using an oligo dT-XhoI primer. Second strand cDNA was prepared using RNase H and DNA polymerase I. EcoR I adaptors were ligated to the cDNA, and it was digested with XhoI. Fragments were size selected, and those between 1-5 kb ligated into EcoRI /XhoI digested vector.
characteristics.characteristic: description
characteristics.text: asynchronous blood stage
characteristics.characteristic: dev-stage
characteristics.text: Swiss white mice
characteristics.characteristic: lab-host
characteristics.text: Plasmodium berghei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5821
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: ANKA clone 15cy1 (clone of the ANKA 8417 HP clone)
characteristics.characteristic: strain
characteristics.text: Pb cDNA #20, Charles Yowell and Jane Carlton
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II vector DNA, excised from Lamda ZAP II.
characteristics.characteristic: vector