characteristics.text: LIBEST_021541
characteristics.characteristic: External Id
characteristics.text: SAMN00153247
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:20.587Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.0kb. This is a non-normalized primary library (normalized library is NIH_MGC_419) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.
characteristics.characteristic: description
characteristics.text: DH10B TonA
characteristics.characteristic: lab-host
characteristics.text: kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organ
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: EcoRV
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: combined sample from 60 day 13 embryos/17 pregnant females
characteristics.characteristic: tissue-type
characteristics.text: NIH_MGC_418
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pExpress-1
characteristics.characteristic: vector