characteristics.text: LIBEST_001643
characteristics.characteristic: External Id
characteristics.text: SAMN00156217
characteristics.characteristic: External Id
characteristics.text: Kwangju Institute of Science & Technology (K-JIST)
characteristics.characteristic: INSDC center name
characteristics.text: 1999-03-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:46.460Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The plasmid vector, pSMASH, is a yeast expression vector derived from pGAD424 (Clonetech). It was designed for selective cloning of the genes encoding secreted and membrane proteins. Briefly, it contains EcoRI-NotI subcloning sites followed by a Kex2 protease recognition site and invertase lacking its own methionine and signal sequence.
characteristics.characteristic: description
characteristics.text: 12-24hr
characteristics.characteristic: dev-stage
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: Drosophila pSMASH Library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSMASH
characteristics.characteristic: vector