characteristics.text: LIBEST_008689
characteristics.characteristic: External Id
characteristics.text: SAMN00163215
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:14.073Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The MI-P-E1 library is derived from whole fetus at gestational day 20. First strand cDNA was primed with a Not I - oligo(dT) primer [5'GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], double-stranded cDNA was size selected, ligated to SalI adapters (BRL), digested with Not I and cloned into the Not I and SalI sites of the pSPORT1 vector (BRL). For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.
characteristics.characteristic: description
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: large white outbred
characteristics.characteristic: strain
characteristics.text: MI-P-E1
characteristics.characteristic: title
characteristics.text: pSPORT1 (BRL)
characteristics.characteristic: vector