characteristics.text: LIBEST_001013
characteristics.characteristic: External Id
characteristics.text: SAMN00155602
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:06.723Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:20:51.607Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with an oligo(dT) primer. Library was constructed in the Hybri-ZAP vector (Stratagene) using 5' and 3' linker adaptors. Average insert size 1.2 kb. This library was constructed by members of the laboratories of Dr. P. Hackett, Dr. M. Halpern, and Dr. S. Ekker.
characteristics.characteristic: description
characteristics.text: up to 512-cell stage
characteristics.characteristic: dev-stage
characteristics.text: XL-1 Blue MRF'/XLOLR
characteristics.characteristic: lab-host
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRI (5')
characteristics.characteristic: re_1
characteristics.text: XhoI (3')
characteristics.characteristic: re_2
characteristics.text: mixed oocytes + embryos
characteristics.characteristic: tissue-type
characteristics.text: Ekker zebrafish maternal
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pAD-GAL4 (Stratagene)
characteristics.characteristic: vector