characteristics.text: LIBEST_011256
characteristics.characteristic: External Id
characteristics.text: SAMN00166048
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2002-09-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:26.793Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PCR products were directly cloned into TA cloning vector (pre-cut vector pCR2.1-TOPO was purchased from Invitrogen) Average insert size is 300bp. Our Insert also have same adaptor sequence on both ends. The sequence of the adaptor is: 5'-ATACGTGCAGGCTGGTTACATCTG-3'.
characteristics.characteristic: description
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: Canis lupus familiaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9615
characteristics.characteristic: organism
characteristics.text: Brain from a dog with encephalitis
characteristics.characteristic: tissue-type
characteristics.text: SV DOGRDA RD3
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pCR2.1-TOPO
characteristics.characteristic: vector