characteristics.text: SAMN00153569
characteristics.characteristic: External Id
characteristics.text: LIBEST_021968
characteristics.characteristic: External Id
characteristics.text: University of Stellenbosch
characteristics.characteristic: INSDC center name
characteristics.text: 2007-10-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:32.097Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA extracted from 200mg Haliotis midae gill tissue with One Step RNA Reagent (Bio Basic Inc). 2ug mRNA isolated with polyA Spin mRNA Isolation Kit (New England Biolabs). First strand synthesis (from 2ug mRNA) primed with Oligo(dT) primer and library construction protocol followed using Universal RiboClone cDNA Synthesis System (Promega). cDNA inserts cloned into pGEM4-Z vector (Promega)
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: JM109 (Promega)
characteristics.characteristic: lab-host
characteristics.text: gill
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002535
characteristics.characteristic: organ
characteristics.text: Haliotis midae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_36098
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Haliotis midae gill cDNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM4-Z (Promega)
characteristics.characteristic: vector