characteristics.text: SAMN00000804
characteristics.characteristic: External Id
characteristics.text: University of Washington Genome Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2008-11-04T15:47:01.973Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:43:14.070Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS001791
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS001791
characteristics.characteristic: SRA accession
characteristics.text: Animals were synchronized by treating a mixed population with bleach to collect embryos. The embryos were incubated without food, causing the larvae to arrest at the L1 stage upon hatching. Starved L1 larvae were plated in the presence of food and raised at 25 degrees Celsius. Staging was determined by examining a subset of animals under Nomarski optics at intervals for vulval and germline development. Animals were collected at mid-L4 stage and washed in S basal buffer. Exact times for growth after plating of starved L1s (all at 25 degrees C) were as follows: L4 for 34.25 hours (vulvae are in Christmas tree stage gonad has passed bend, sperm are present)
characteristics.characteristic: description
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Generic sample from Caenorhabditis elegans
characteristics.characteristic: title