characteristics.text: LIBEST_020647
characteristics.characteristic: External Id
characteristics.text: SAMN00152442
characteristics.characteristic: External Id
characteristics.text: James Cook University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-09-05T21:03:40.344Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Isolated from the coral Acropora aspera. Symbiodinium were removed from the coral skeleton and then harvested by centrifugation, washed four time in filtered sea water before being ground under liquid nitrogen and resuspended in RLT buffer (Qiagen RNA Midi Prep Kit). Cells were then spun through a Qia-Shredder (Qiagen) and snap frozen in liquid nitrogen for storage. Total RNA was purified for each sample using the Qiagen mRNA Midi Prep Kit, equal amounts of total RNA from each treatment (control, short term heat stress, long term heat stress, ammonium enriched and isolated/Ci treated) were mixed and the mRNA was purified using a Poly A Purist kit (Ambion). The purified mRNA was then used to construct a directional phage library in lambda-ZAP II. Phagemids were generated and the library was normalised using the method of Bonaldo et al. (1996) and clones were sequenced directionally using the M13 Reverse primer.
characteristics.characteristic: description
characteristics.text: Cladocopium sp. C3
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2558455
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: C3
characteristics.characteristic: strain
characteristics.text: Symbiodinium normalized stress library Lambda ZAP II
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector