characteristics.text: LIBEST_000384
characteristics.characteristic: External Id
characteristics.text: SAMN00154996
characteristics.characteristic: External Id
characteristics.text: University of Padua
characteristics.characteristic: INSDC center name
characteristics.text: 1995-12-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.973Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by G. Lanfranchi. This library is not subtracted nor normalized. The first strand cDNA was primed with a biotinylated oligo-dT-NotI primer~(5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The ds cDNA was sonicated and size-selected in the range 250-400 bp. The 3' specific fragments were selected by streptavidin coated magnetic beads, ligated to non-palindromic BstXI adapters, NotI digested and directionally cloned into BstXI-NotI cut pcDNAII vector.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: pectoral muscle (after mastectomy)
characteristics.characteristic: tissue-type
characteristics.text: HM1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pcDNAII (Invitrogen)
characteristics.characteristic: vector