characteristics.text: LIBEST_009748
characteristics.characteristic: External Id
characteristics.text: SAMN00164254
characteristics.characteristic: External Id
characteristics.text: Ocean University of Qingdao
characteristics.characteristic: INSDC center name
characteristics.text: 2002-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:45.803Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Wild type Gracilaria lemaneiformis were harvested from Zhanshan Bay, Qingdao (China). After rinsed with boiled seawater, younger thalli was cut and washed every 3-4 days until 1 month. Then thalli was cultivated in Provasoli medium. Total RNA was isolated from thalli of gametophyte algae, using UNIQ-10 Trizol Total RNA Preparation Kit(Sangon Inc., Shanghai, China). The cDNA was synthesized, amplified and cloned using cDNA Synthesis Kit, cDNA PCR Library Kit and pMD 18-T vector (TaKaRa Biotechnology Co., Ltd., Dalian, China), respectively.
characteristics.characteristic: description
characteristics.text: Gracilariopsis lemaneiformis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2782
characteristics.characteristic: organism
characteristics.text: EcoR V with a T hang
characteristics.characteristic: re_1
characteristics.text: gametophyte thalli
characteristics.characteristic: tissue-type
characteristics.text: Gracilaria lemaneiformis gametophyte cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pMD 18-T
characteristics.characteristic: vector