characteristics.text: LIBEST_020577
characteristics.characteristic: External Id
characteristics.text: SAMN00152363
characteristics.characteristic: External Id
characteristics.text: National Eye Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2006-11-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:53.427Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA was extracted from pooled rhesus monkey lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: EMDH10B
characteristics.characteristic: lab-host
characteristics.text: Eye
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000970
characteristics.characteristic: organ
characteristics.text: Macaca mulatta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9544
characteristics.characteristic: organism
characteristics.text: Lens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000965
characteristics.characteristic: tissue-type
characteristics.text: Rhesus monkey lens. Unnormalized (nbl)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSport6
characteristics.characteristic: vector