characteristics.text: LIBEST_016553
characteristics.characteristic: External Id
characteristics.text: SAMN00154144
characteristics.characteristic: External Id
characteristics.text: Teagasc, Agriculture and Food Development Authority
characteristics.characteristic: INSDC center name
characteristics.text: 2005-06-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:56.100Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A bovine ovarian cortex cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5(g of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum. The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.
characteristics.characteristic: description
characteristics.text: Day 2, 6, 8 and 14 of the oestrus cycle
characteristics.characteristic: dev-stage
characteristics.text: Ovarian cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0013191
characteristics.characteristic: organ
characteristics.text: Bos taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Ovarian cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0013191
characteristics.characteristic: tissue-type
characteristics.text: Bovine Lambda Zap Express ovarian cortex cDNA library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP Express
characteristics.characteristic: vector