characteristics.text: SAMN00156101
characteristics.characteristic: External Id
characteristics.text: LIBEST_001523
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-01-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:42.490Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT)15 primer [5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3']; double-stranded cDNA was ligated to Sal I adaptors (BRL), digested with Not I and cloned into the Not I and Sal I sites of the pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab; ICRF, London and Max Planck Institut fuer Molekulare Genetik,Berlin). cDNAs for EST analysis were selected following oligonucleotide hybridization fingerprinting of arrayed clones from zebrafish late somitogenesis (26 ss), adult liver or embryonic shield stage (5.6 h) libraries. Fingerprint data were used to computationally cluster cDNAs, and a single cDNA from each cluster was chosen for sequencing. In some cases multiple members of the same cluster were sequenced to assess clustering parameters or single clones were sequenced additional times to assess quality control.
characteristics.characteristic: description
characteristics.text: XL1-blue MRF
characteristics.characteristic: lab-host
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: 26 somite embryos, adult livers, shield stage embryos
characteristics.characteristic: tissue-type
characteristics.text: Zebrafish WashU MPIMG EST
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector