characteristics.text: SAMN00157055
characteristics.characteristic: External Id
characteristics.text: LIBEST_002494
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 1999-10-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:08.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Jemalong, line A17
characteristics.characteristic: cultivar
characteristics.text: At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this 30 day period, they were fertilized twice weekly with 1/2 Hoaglands solution containing only 20uM potassium phosphate. RNA was prepared from the roots. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells.
characteristics.characteristic: description
characteristics.text: phosphate starved
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: roots
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: tissue-type
characteristics.text: rootphos(-)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK -
characteristics.characteristic: vector