characteristics.text: SAMN00154362
characteristics.characteristic: External Id
characteristics.text: LIBEST_020417
characteristics.characteristic: External Id
characteristics.text: Mount Desert Island Biological Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:48.283Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The rectal gland was dissected from an adult spiny dogfish shark. Total RNA was extracted using TRIzol reagent followed by mRNA isolation. The primary cDNA library was directionally cloned in DH10B T1 and normalized by Invitrogen. The average insert size was 2.4 kb. Inserts were single-pass sequenced from the 5' end by Rexagen (now High-Throughput Sequencing Solutions, University of Washington, Seattle, WA). Traces were trimmed and prepared for submission to dbEST using trace2dbest software (Parkinson, Anthony and Blaxter, unpublished). This expressed sequence tag project was supported by NIH Grant number 1 R01 019732 from the National Center for Research Resources (D.W. Barnes, PI), the Woodcock Foundation (D.W. Barnes and C.J. Bayne, PI) and P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).
characteristics.characteristic: description
characteristics.text: rectal gland
characteristics.characteristic: organ
characteristics.text: Squalus acanthias
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7797
characteristics.characteristic: organism
characteristics.text: not determined
characteristics.characteristic: sex
characteristics.text: entire
characteristics.characteristic: tissue-type
characteristics.text: Spiny dogfish shark rectal gland EST library
characteristics.characteristic: title
characteristics.text: pCMV-SPORT6.1
characteristics.characteristic: vector