characteristics.text: LIBEST_016269
characteristics.characteristic: External Id
characteristics.text: SAMN00166145
characteristics.characteristic: External Id
characteristics.text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry.
characteristics.characteristic: INSDC center name
characteristics.text: 2004-08-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:47.480Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Himalaya
characteristics.characteristic: cultivar
characteristics.text: mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.
characteristics.characteristic: description
characteristics.text: developing endosperm tissue 10, 12, 15 dpa (days post anthesis)
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technology)
characteristics.characteristic: lab-host
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_112509
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: endosperm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009089
characteristics.characteristic: tissue-type
characteristics.text: Barley EST endosperm library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: _ZipLox
characteristics.characteristic: vector