characteristics.text: LIBEST_019350
characteristics.characteristic: External Id
characteristics.text: SAMN00150010
characteristics.characteristic: External Id
characteristics.text: Concordia University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-03-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-02-22T21:02:06.595Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Sporotrichum thermophile cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).
characteristics.characteristic: description
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Thermothelomyces heterothallicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1149786
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Sporotrichum thermophile pBluescript (EcoRI-XhoI)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript KS+
characteristics.characteristic: vector