characteristics.text: LIBEST_013014
characteristics.characteristic: External Id
characteristics.text: SAMN00153981
characteristics.characteristic: External Id
characteristics.text: UC Davis, Plant Pathology
characteristics.characteristic: INSDC center name
characteristics.text: 2003-04-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:12.550Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cabernet Sauvignon
characteristics.characteristic: cultivar
characteristics.text: CAB1 is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 flowers. Samples were collected approximately eleven days before onset of bloom (clusters at this stage were fully developed and flowers with calyptras or caps still attached. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.
characteristics.characteristic: description
characteristics.text: Pre-bloom
characteristics.characteristic: dev-stage
characteristics.text: DH5alpha
characteristics.characteristic: lab-host
characteristics.text: Flower - Pre-bloom
characteristics.characteristic: organ
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Cabernet Sauvignon Flower Pre-bloom - CAB1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR
characteristics.characteristic: vector