characteristics.text: SAMN00155997
characteristics.characteristic: External Id
characteristics.text: LIBEST_001418
characteristics.characteristic: External Id
characteristics.text: International Livestock Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 1998-09-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:39.997Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cultured bovine lymphocytes infected with Theileria parva
characteristics.characteristic: cell type
characteristics.text: A cDNA library enriched in T. parva schizonts was constructed using mRNA derived from cultured bovine lymphoblastoid cells that were infected with T. parva Mguga schizonts. Theileria parva schizonts from 10 million infected bovine macrophages were concentrated by centrifugation and total RNA was extracted from this schizonts-enriched fraction using the hot phenol-SDS method, (Murphy and Pelle, 1995). Poly-A+ RNA was obtained from the total RNA by oligo-dT cellulose (Phamacia Biotech) affinity chromatograph. Complementary DNA was synthesised from the poly-A+ RNA using the RiboClone cDNA Synthesis Systems (Promega) and standard methods. To facilitate cloning, the cDNAs were methylated using EcoRI methylase and ligated to oligonucleotide adapters containing the EcoRI site (GGGGGAATTCGGGGG) using T4 DNA ligase (NEB). The cDNAS were then treated with the restriction endonuclease EcoRI (NEB), purified and then ligated to lgt11 bacteriophage vector arms (Promega) digested with the same enzyme. Recombinant lgt11 phage DNA was packaged into phage capsids using the Packagene l-DNA packaging mixture (Promega) and amplified by infecting E. coli, strain Y1090 cells.
characteristics.characteristic: description
characteristics.text: Schizont stage
characteristics.characteristic: dev-stage
characteristics.text: Theileria parva
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5875
characteristics.characteristic: organism
characteristics.text: Muguga
characteristics.characteristic: strain
characteristics.text: Bovine lymphocytes
characteristics.characteristic: tissue-type
characteristics.text: Schizont Lambda gt11 cDNA Library
characteristics.characteristic: title