characteristics.text: SAMN00165291
characteristics.characteristic: External Id
characteristics.text: LIBEST_022743
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-02-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:53.500Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: Stressed by exposure to Microcystis. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: TOP10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007796
characteristics.characteristic: lab-host
characteristics.text: Daphnia pulex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6669
characteristics.characteristic: organism
characteristics.text: Sfi I A
characteristics.characteristic: re_1
characteristics.text: Sfi I B
characteristics.characteristic: re_2
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Log50 (arenata)
characteristics.characteristic: strain
characteristics.text: whole animal
characteristics.characteristic: tissue-type
characteristics.text: CAOA_Daphnia_pulex_Log50_Library_16
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector