characteristics.text: LIBEST_019764
characteristics.characteristic: External Id
characteristics.text: SAMN00150379
characteristics.characteristic: External Id
characteristics.text: Southwest Foundation for Biomedical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:26.757Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Forward and reverse subtractive hybridizations were performed with intestinal cDNAs from an opossum with elevated plasma low density lipoprotein cholesterol and intestinal cDNAs from an opossum with normal plasma low density lipoprotein cholesterol using the PCR-Select cDNA subtraction kit (Clontech). The subtracted cDNAs were cloned into the pCR4-TOPO vector (Invitrogen), and the ligation mixtures were transformed into TOP10 cells.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Monodelphis domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13616
characteristics.characteristic: organism
characteristics.text: small intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002108
characteristics.characteristic: tissue-type
characteristics.text: Opossum subtracted intestinal cDNA libraries
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR4-TOPO
characteristics.characteristic: vector