characteristics.text: SAMN00154051
characteristics.characteristic: External Id
characteristics.text: LIBEST_014693
characteristics.characteristic: External Id
characteristics.text: Concordia University, Department of Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2003-12-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:02.353Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Conditions for growth: Seeds were germinated in a water-saturated mix (50% black earth and 50% ProMix) in a growth chamber for 7 days under an irradiance of 200 mmol m-2 sec-1. The temperature was maintained at 20 degrees C with a 15-hr photoperiod under a relative humidity of 70%. After this period watering of plants was stopped. Four time points were sampled during a two week period; the first after wilting was observed and the last, two weeks later, consisted of live crown and leaf tissue (leaf tissue that was yellow was not included in sampled material).First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.
characteristics.characteristic: description
characteristics.text: Crown and leaf
characteristics.characteristic: organ
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: Triticum aestivum FGAS: Library 4 Gate 8
characteristics.characteristic: title
characteristics.text: pCMV.SPORT6
characteristics.characteristic: vector