characteristics.text: SAMN00153458
characteristics.characteristic: External Id
characteristics.text: LIBEST_021806
characteristics.characteristic: External Id
characteristics.text: University of Muenster, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:27.910Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: six randomly chosen genotypes from a wild population at ambient winter temperature of 3C. lat_lon: 54.82 N 10.81 E. collection_date: Feb-2005. Total RNA was extracted from young leaf tissue and meristematic region of plants at ambient temperature of 3C (February 2005) collected in wild by SCUBA. The cleaned tissue was immediately shock frozen (<10min after collection) nd stored at -80C. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial LD-PCR amplification of 28 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Only 50% of bacterial clones have insert, therefore we included an initial PCR based size selection prior sequencing. Sequencing based on Plasmid preps using the primer GTA AAA CGA CGG CCA GT at Max-Planck-Institute for Limnology, Dept Ecophysiology, August-Thienemann-Str. 2, 24306 Ploen, Germany Contact for clones: treusch@uni-muenster.de, Institut for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149 Muenster, Germany.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Zostera marina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29655
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: ZM_D
characteristics.characteristic: title