characteristics.text: SAMN00164316
characteristics.characteristic: External Id
characteristics.text: LIBEST_009810
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-09-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:47.320Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygene and Public Health at John Hopkins University in Baltimore, MD.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Ascaris suum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6253
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Head
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000033
characteristics.characteristic: tissue-type
characteristics.text: Ascaris suum male head pAMP1 v2 Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pAMP1 (Gibco)
characteristics.characteristic: vector