characteristics.text: LIBEST_022844
characteristics.characteristic: External Id
characteristics.text: SAMN00165388
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:57.057Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: In Vivo Conditions: broad array of treatments including thermal stress, salinity stress, heavy metal stress, selenium stress, insecticide stress, nutritional stress, wild-caught specimens, laboratory acclimated specimens. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found in the Porcelain Crab Array Database; http://array.sfsu.edu.
characteristics.characteristic: description
characteristics.text: pool of adult, larval, freshly molted
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Petrolisthes cinctipes
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_88211
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: pooled heart, gills, whole crab
characteristics.characteristic: tissue-type
characteristics.text: CCAG Petrolisthes cinctipes heart, gills, whole crab (N)
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector