characteristics.text: LIBEST_019301
characteristics.characteristic: External Id
characteristics.text: SAMN00149954
characteristics.characteristic: External Id
characteristics.text: Universidade Federal de Sao Paulo - Escola Paulista de Medicina
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:15.027Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The poly A+ RNA was isolated by chromatography on oligo(dT) cellulose column. The cDNA synthesis was carried out using the SuperScript Lambda System for cDNA Synthesis and l Cloning. The cDNA molecules higher than 500 bp were inserted into lambda ZipLox Not I/Sal I Arms vector and packaged using Gigapack III Gold Packaging Extract.
characteristics.characteristic: description
characteristics.text: amastigote
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Leishmania amazonensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5659
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: LEM690 (MHOM/BR/1973/M2269)
characteristics.characteristic: strain
characteristics.text: Leishmania (Leishmania) amazonensis amastigote cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZipLox Not I / Sal I Arms Vector
characteristics.characteristic: vector