characteristics.text: SAMN00158577
characteristics.characteristic: External Id
characteristics.text: LIBEST_004024
characteristics.characteristic: External Id
characteristics.text: Duke University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:48.817Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library combines cDNAs from nutrient replete (TAP medium, mid-log phase), sulfur-deprived (TAP medium -S for 2 hr), and iron-deprived cells from CC-125. Fe-deprived cells were obtained by either (1) growth in SGII medium at 0.18 micromolar Fe, 2X wash with SGII -Fe and resuspension in SGII -Fe + 80 micromolar bathophenanthroline sulfonic acid (an iron chelator) for 2 hours or (2) growth in SGII medium, 2X wash with SGII -Fe, resuspension in SGII -Fe and continued growth for 48 hours. 1.25 micrograms of each polyA mRNA prep was used to synthesize cDNA which was directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage.
characteristics.characteristic: description
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: CC-125 wild type mt+ 137C
characteristics.characteristic: strain
characteristics.text: C. reinhardtii CC-125 nutrient replete, -S, -Fe, Lambda Zap II
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK-
characteristics.characteristic: vector