characteristics.text: SAMN00150136
characteristics.characteristic: External Id
characteristics.text: LIBEST_019509
characteristics.characteristic: External Id
characteristics.text: Okazaki National Research Institutes
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:20.387Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Fly gonads were isolated from embryos carrying EGFP-vasa transgene that express GFP only in poll cells by using fluorescence-activated cell sorting (FACS). Total RNA was extracted from the isolated gonads at embryonic stages 13-16 by using RNeasy Mini kit (QIAGEN). Poly(A)+ RNA was purified Oligotex dT30 (TaKaRa) from the total RNA. cDNA was synthesized and amplified by Long-Distance PCR using SMART technology (Clontech) and directionally cloned into pDNR-LIB vector (Clontech) cDNA inserts are flanked by linker sequences 5'-GGCCATTACGGCCGGG-3' (5' linker: SMART IV Oligonucleotide, Clontech) and 5'-polyA-CATGTCGGCCGCCTCGGCC-3' (3' linker: CDSIII/3' PCR Primer, Clontech).
characteristics.characteristic: description
characteristics.text: 10-18 hours embryo (stage 13-16)
characteristics.characteristic: dev-stage
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: mixture of male and female
characteristics.characteristic: sex
characteristics.text: vasa-EGFP
characteristics.characteristic: strain
characteristics.text: gonad
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000991
characteristics.characteristic: tissue-type
characteristics.text: Drosophila melanogaster embryonic gonads cDNA (pDNR-LIB)
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector