characteristics.text: SAMN00152100
characteristics.characteristic: External Id
characteristics.text: LIBEST_020360
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-Food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:46.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suppression subtractive hybridisation (SSH) was performed according to the manufacturer's instructions using the PCR-based cDNA subtraction kit (Clontech, Mountain View, USA). Thlaspi arvense was grown on 0.5X MS media at 18 oC until the 3-4 leaf stage. The aerial tissue from half of the seedlings was used to prepare RNA to represent untreated tissue (driver) and half were treated at 3 oC for 4 hours before RNA extraction (tester).
characteristics.characteristic: description
characteristics.text: Thlaspi arvense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13288
characteristics.characteristic: organism
characteristics.text: Leaf tissue from plants at four leaf stage
characteristics.characteristic: tissue-type
characteristics.text: Thlaspi arvense SSH library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector