characteristics.text: SAMN00155250
characteristics.characteristic: External Id
characteristics.text: LIBEST_000651
characteristics.characteristic: External Id
characteristics.text: Smith College Department of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1997-04-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:16.580Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Filarial nematode parasite of humans. Third-stage larvae, L3, were isolated from infected black flies in Cameroon (forest strain). The L3 were cultured in 20% FCS in IMDM+ NCTC 135 and collected after day 1, 2, or 3 in culture. L3 of O. volvulus molt to fourth-stage larvae by day 5 in culture. mRNA was isolated from approximately 6000 molting larvae (mL3),2000 larvae from day 1, 2 or 3 in culture, and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library was constructed in the lambda Uni-Zap XR vector and has 1 x 10E6 independent recombinants and the average insert size is ~1200 bp. The library was constructed by Sara Lustigman and Michelle Lizotte-Waniewski in the Laboratory of Dr. S. A. Williams. The library is available from Dr. Sara Lustigman (email: slustigm@nybc.org).
characteristics.characteristic: description
characteristics.text: molting L3
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Onchocerca volvulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6282
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: Kumba, Cameroons
characteristics.characteristic: strain
characteristics.text: Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR
characteristics.characteristic: vector