characteristics.text: SAMN00003050
characteristics.characteristic: External Id
characteristics.text: Bangor University
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-27T08:59:19.830Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-09-18T13:23:47.090Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS004781
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS004781
characteristics.characteristic: SRA accession
characteristics.text: These sequences derive from 454 sequencing of nSSU 18S amplicons from environmental sediment samples from Prestwick and Littlehampton (UK 2007) low tide sites. The amplicon genomic region is defined by the primers SSUFO4 (5’-GCTTGTAAAGATTAAGCC-3’) and SSUR22 (5’- GCCTGCTGCCTTCCTTGGA-3’) and the following MID tag oligos were used to label each independent sample: GTGAG Prestwick_1A TACGC Prestwick_1B GTCAC Prestwick_1C CTACT Prestwick_2ABC TCTGT Prestwick_3ABC GATGA Prestwick_4ABC TGACT Prestwick_5ABC ATGCT Prestwick_6ABC TCGCT Prestwick_7ABC TATAC Prestwick_8ABC ACGAC Littlehampton_1A TCGCT Littlehampton_1B TACAT Littlehampton_1C Therefore, following 454 processing, each sequence should commence with the 5bp identifying tag, followed by the primer SSUF04, followed by the organismal sequence data.
characteristics.characteristic: description
characteristics.text: sediment metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_749907
characteristics.characteristic: organism
characteristics.text: Generic sample from Eukaryota
characteristics.characteristic: title