characteristics.text: SAMN00017363
characteristics.characteristic: External Id
characteristics.text: Università degli Studi di Verona
characteristics.characteristic: INSDC center name
characteristics.text: 2011-03-28T18:10:46.063Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:58:19.160Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS085324
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS085324
characteristics.characteristic: SRA accession
characteristics.text: For the host-recognition stage, equal volumes of conidial suspensions containing the aforementioned concentrations of both A. quisqualis and P. xanthii were mixed and incubated for 24 hours at 25*C. Then, the conidia (50 mg) were collected by filtration on Purabind 08 filter paper (Whatmann; Dassel, Germany), frozen in liquid nitrogen and lyophilized (Heto LyoLab 3000, Thermo Scientific Heraeus; Loughborough, UK). For the early stages of parasitization, 10 ml of P. xanthii conidial suspension (1 * 104 conidia/ml) were sprayed on 30 zucchini plants that each had five true leaves. Plants were left to dry and then incubated overnight at 22*C and high RH (95-98%). When powdery mildew symptoms first appeared (14 days after inoculation), 15 of the plants were treated with 10 ml of A. quisqualis suspension (3.5 * 106 conidia/ml; 10 ml/plant) and 15 were sprayed with water (untreated control). The host-recognition and early-parasitization stages samples were pooled together, and the same quantity of A. quisqualis total RNA was added for each of the two stages (0.2 *g per pool).
characteristics.characteristic: description
characteristics.text: Ampelomyces quisqualis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_50730
characteristics.characteristic: organism
characteristics.text: pool of A. quisqualis RNA from the host-recognition and early-parasitization stages
characteristics.characteristic: title