characteristics.text: SAMN00153020
characteristics.characteristic: External Id
characteristics.text: LIBEST_021287
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-13T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:14.363Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The mid-developmental stage female flower (cone) of Hops (Humulus lupulus) plants, cultivar Phoenix, were collected form plants grown at the Hop Research Institite, Wye, Kent, UK. The cones were stripped off the branches, and the largest cones (which were unlikely to develop much further), and the smaller cones (which contain few trichomes) were discarded. Total RNA isolated from hops trichome prepared by shaking the cones in a 50 mL tubes by using Cold-Phenol protocol. The cDNA library from hops trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA.
characteristics.characteristic: description
characteristics.text: female flower at mid-developmental stage
characteristics.characteristic: dev-stage
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: Sfi IA (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: Sfi IB (GGCCGCCTCGGCC)
characteristics.characteristic: re_2
characteristics.text: Trichome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000282
characteristics.characteristic: tissue-type
characteristics.text: Humulus lupulus (L. cultivar Phoenix) Trichome isolated from the female flower at mid-developmental stage
characteristics.characteristic: title
characteristics.text: pDNR-LIB
characteristics.characteristic: vector