characteristics.text: SAMN00165711
characteristics.characteristic: External Id
characteristics.text: LIBEST_023134
characteristics.characteristic: External Id
characteristics.text: UMR 7100 - IFR 85 - ESBS -ULP
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:05.487Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: cultivar
characteristics.text: Total RNA exctraction was performed with the RNaqueous-4PCR kit (Ambion, USA). Poly(A)+ RNA was purified with the Oligo Tex Mini kit (Qiagen, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate the specific cDNA library. Tester cDNA was prepared from wild type male flowers and driver cDNA was from hermaphrodite flowers (Crossbreeding between tetraploid Strisselspalt (female) and Challenger (male)). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).
characteristics.characteristic: description
characteristics.text: E.coli TOP10 F'
characteristics.characteristic: lab-host
characteristics.text: Flower
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009046
characteristics.characteristic: organ
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: HincII
characteristics.characteristic: re_1
characteristics.text: HincII
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Humulus lupulus Male-Hermaphrodite differential cDNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC19
characteristics.characteristic: vector