characteristics.text: SAMN00166010
characteristics.characteristic: External Id
characteristics.text: LIBEST_001886
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular and Cell Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2000-01-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-07-03T23:05:03.420Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Bacilli were disrupted using a Mini bead beater (Biospec). RNA was isolated using the RNeasy protocol (Qiagen). Purified RNA was subjected to 2 rounds of digestion with RNase-free DNase I (Promega). DNase I was heat-inactivated at 75oC for 5 min. and removed by using RNeasy columns followed by phenol extraction and ethanol precipitation. The RNA preparations were confirmed to be free of genomic DNA contamination by carrying out PCR and RT-PCR using the Access kit (Promega) and primers specific for the histone-like protein gene hlp (Lee et al., 1998). cDNA was synthesized using random hexamer primers (Promega) and Stratagene's cDNA synthesis kit. cDNA fragments were ligated into lambda ZAP II vector and packaged in vitro using Stratagene reagents.
characteristics.characteristic: description
characteristics.text: E. coli XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Mycolicibacterium smegmatis MC2 155
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_246196
characteristics.characteristic: organism
characteristics.text: mc2155
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002860
characteristics.characteristic: strain
characteristics.text: Mycobacterium anaerobic stationary phase library
characteristics.characteristic: title
characteristics.text: Lambda ZAP II
characteristics.characteristic: vector