characteristics.text: LIBEST_014633
characteristics.characteristic: External Id
characteristics.text: SAMN00154046
characteristics.characteristic: External Id
characteristics.text: Faculty of Biological Sciences, Catholic University of Chile
characteristics.characteristic: INSDC center name
characteristics.text: 2003-11-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:59.733Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Chardonnay
characteristics.characteristic: cultivar
characteristics.text: From leaves total RNA were isolated as described in Plant Mol Biol Reporter 11 (1993) 212-215. From berries total RNA were isolated using CTAB method and lithium chloride precipitation (Plant Mol Biol Reporter 11 (1993) 117-121 ). Double stranded cDNA were prepared from 5 ug of polyA leaf RNA or 25 ug total RNA berry RNA using a Stratagene cDNA synthesis kit. This methods produces hemymethilated cDNAs with 5'EcoRI and 3'XhoI restriction sites. These cDNA were cloning in pBluescript SK II+. Following ligation, plasmids were transformed into XL1-Blue-MRF'cells (Stratagene) and plated for blue-white selection .
characteristics.characteristic: description
characteristics.text: leaf and berry
characteristics.characteristic: organ
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: 5' Eco R I
characteristics.characteristic: re_1
characteristics.text: 3' Xho I
characteristics.characteristic: re_2
characteristics.text: grape selected express library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector