characteristics.text: LIBEST_020363
characteristics.characteristic: External Id
characteristics.text: SAMN00152103
characteristics.characteristic: External Id
characteristics.text: University of New Mexico
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:46.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA, pooled from 20 M-line B. glabrata snails, injected with Micrococcus luteus (12 hours), was used as template for RT-PCR with random primers (18nt) to generate ORESTES(ORF-ESTs). Resulting cDNAs were shotgun cloned (pCR2.1 TOPO TA, Invitrogen), and sequenced using primer M13(-20). campo = C Adema M-line (Gram) Positive Orestes. Random primers were removed from sequence as presented.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: M-line
characteristics.characteristic: strain
characteristics.text: whole body
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001489
characteristics.characteristic: tissue-type
characteristics.text: campo
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector