characteristics.text: LIBEST_022343
characteristics.characteristic: External Id
characteristics.text: SAMN00164578
characteristics.characteristic: External Id
characteristics.text: Massachusetts Institute Of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:41.850Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Whole animal
characteristics.characteristic: cell type
characteristics.text: RNA Ligase-mediated 5' RACE (RLM-RACE) product for ceh-30 (C33D12.7) from total RNA of a mixed stage population of him-5(e1467) (CB1467) C. elegans. Total RNA was dephosphorylated, the phosphorylase was inactivated, the mRNA was decapped, and a DNA-RNA hybrid oligonucleotide linker was ligated to the phosphorylated 5' ends of processed mRNAs that had been rendered available by decapping. Reverse transcription performed using a gene-specific primer whose sequence is given in the comments of the EST sequence file and amplified by PCR using this same gene-specific primer and a primer specific to the oligonucleotide linker. Products were reamplified using a second gene-specific primer whose sequence is given in the comments of the EST sequence file and a second, nested primer specific to the oligonucleotide linker. Products were ligated into pGEM T-Easy. Individual transformants were picked, miniprepped, and sequenced using SP6 and T7 primers. This sequence file has been trimmed to remove primer and vector sequences.
characteristics.characteristic: description
characteristics.text: Mixed stage population
characteristics.characteristic: dev-stage
characteristics.text: Whole animal
characteristics.characteristic: organ
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Mixed males and hermaphrodites
characteristics.characteristic: sex
characteristics.text: CB1467
characteristics.characteristic: strain
characteristics.text: Whole animal
characteristics.characteristic: tissue-type
characteristics.text: CB1467 Total RNA RLM-RACE
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM T-Easy
characteristics.characteristic: vector