characteristics.text: LIBEST_008942
characteristics.characteristic: External Id
characteristics.text: SAMN00163455
characteristics.characteristic: External Id
characteristics.text: Universiti Kebangsaan Malaysia
characteristics.characteristic: INSDC center name
characteristics.text: 2001-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:21.267Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sporozoites were excysted in vitro from E. tenella H oocysts, purified by column chromatography and mRNA extracted using a FASTTRACK kit (Invitrogen). cDNA was synthesised and a Uni-ZAP XR library was constructed using cDNA synthesis kit, ZAP-cDNA synthesis kit and ZAP-cDNA Gigapack III Gold cloning kit (Stratagene). The library was amplified once through E. coli XL1-Blue MRF'.
characteristics.characteristic: description
characteristics.text: Sporozoite
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Eimeria tenella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5802
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Houghton
characteristics.characteristic: strain
characteristics.text: EtH11
characteristics.characteristic: title
characteristics.text: Lambda ZAPII
characteristics.characteristic: vector