characteristics.text: LIBEST_023706
characteristics.characteristic: External Id
characteristics.text: SAMN00166665
characteristics.characteristic: External Id
characteristics.text: NRC Plant Biotechnology Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:23.140Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: cultivar
characteristics.text: The library HLUPJN1 was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2002-2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using buffered phenol-chloroform-isoamyl alcohol followed by a clean-up with an RNeasy Plant Mini Kit (Qiagen); mRNA was purified using an Oligotex kit (Qiagen). The library was constructed using a SMART kit (Clontech). cDNAs were ligated into the phage vector lambda-TriplEx2 and packaged with MaxPlax Lambda packaging extract (Epicentre Technologies). For sequencing, cDNA inserts were PCR amplified from phage plaques using the vector primers 5'-CAAGCTCCGAGATCTGGACGAGC-3 and 5'-ATACGACTCACTATAGGGCGAATTGGCC-3'.
characteristics.characteristic: description
characteristics.text: Trichomes isolated from mid to ripe stage hop cones
characteristics.characteristic: dev-stage
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: HLUPJN1
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: lambda-TriplEx2 (Clontech)
characteristics.characteristic: vector