characteristics.text: SAMN00152905
characteristics.characteristic: External Id
characteristics.text: LIBEST_021163
characteristics.characteristic: External Id
characteristics.text: Oregon State University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:11.187Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA library was obtained from Douglas-fir seedlings in their first year of growth. All seedlings were grown outdoors in Corvallis, Oregon, USA and collected from February 2004 to April 2004. Total RNA was extracted from ~3g of ground tissue (separately from buds, stems, and needles) from a sample of 10 seedlings, following a modified protocol described in Chang et al. (1993). Total RNA was used by Evrogen JCS (Moscow, Russia) for ds cDNA synthesis, using the SMART approach (Zhu et al. 2001), and normalized using the DSN normalization method (Zhulidov et al. 2004). cDNA was directionally inserted into the pAL17.1 vector and transformed into E. coli with the BioRad Micropulser (Hercules, CA). cDNA clones were sequenced by SymBio Corporation (Menlo Park, CA).
characteristics.characteristic: description
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: dev-stage
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Pseudotsuga menziesii var. menziesii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_278161
characteristics.characteristic: organism
characteristics.text: AscI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: needles, stems, and buds
characteristics.characteristic: tissue-type
characteristics.text: Douglas-fir cold deacclimating cDNA library 2003-2004 (CD_2003-04)
characteristics.characteristic: title
characteristics.text: pAL17.1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector