characteristics.text: SAMN00154064
characteristics.characteristic: External Id
characteristics.text: LIBEST_015133
characteristics.characteristic: External Id
characteristics.text: Universite Laval
characteristics.characteristic: INSDC center name
characteristics.text: 2004-01-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:13.887Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Tissues were pooled from above and below the girdle. cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation
characteristics.characteristic: description
characteristics.text: Vascular cambium, secondary phloem, and bark from trees harvested 1 and 7 days after girdling treatment
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Main stem of 4 year old saplings, approximately 60 cm tall
characteristics.characteristic: organ
characteristics.text: Picea glauca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3330
characteristics.characteristic: organism
characteristics.text: Eco-RI
characteristics.characteristic: re_1
characteristics.text: Xho-I
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: pg-653
characteristics.characteristic: strain
characteristics.text: Vascular cambium, secondary phloem and bark of trees girdled by removing a ring of bark ca. 1 cm wide from the midpoint of the main stem
characteristics.characteristic: tissue-type
characteristics.text: GQ008: Cambium, phloem and bark of girdled saplings
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector