characteristics.text: LIBEST_008629
characteristics.characteristic: External Id
characteristics.text: SAMN00163155
characteristics.characteristic: External Id
characteristics.text: Institute of Bioorganic Chemistry of Polish Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:12.320Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ventus
characteristics.characteristic: cultivar
characteristics.text: cDNA was prepared from polyA+ enriched RNA from roots with developing nodules harvested seven days post inoculation with Bradyrhizobium sp. WM9 (Lupinus). The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells.
characteristics.characteristic: description
characteristics.text: roots with immature nodules harvested seven days post inoculation with Bradyrhizobium sp. WM9 (Lupinus)
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain SOLR
characteristics.characteristic: lab-host
characteristics.text: Lupinus luteus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3873
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: roots with young nodules
characteristics.characteristic: tissue-type
characteristics.text: LlN01
characteristics.characteristic: title
characteristics.text: pBluescript SK-
characteristics.characteristic: vector