characteristics.text: LIBEST_023369
characteristics.characteristic: External Id
characteristics.text: SAMN00165939
characteristics.characteristic: External Id
characteristics.text: Istituto Agrario di San Michele all'Adige (IASMA)
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:13.187Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Pinot Noir, Moscato Bianco, Teroldego, Freiburg 933/60 (rootstock)
characteristics.characteristic: cultivar
characteristics.text: Total RNA from grape berry (Pinot Noir, Moscato Bianco, Teroldego, rootstock Freiburg 933/60), leaf and root (Pinot Noir) tissues were reverse transcribed in double-stranded cDNA using the SMART PCR cDNA synthesis kit (Clontech) as described by the manufacturer. A berry subtracted library was constructed using the suppression subtractive hybridization (SSH) technique performed with the PCR-Select cDNA Subtraction Kit (Clontech) according to the manufacturer's procedure. In details, after adaptor ligation, tester cDNA from berry tissue was hybridized with driver cDNA consisting of leaf and root cDNAs in equal parts. Two rounds of hybridizations and PCR amplifications were performed to enrich in berry-specific transcripts. The subtracted cDNAs amplified in the secondary PCR were cloned into the pCR 2.1-TOPO vector (Invitrogen) by means of TA cloning strategy and transformed in One Shot TOP10 Eletrocompetent E.coli (Invitrogen) cells. Positive transformants based on blue/white color selection were grown at 37 C in 384-well plates containing LB medium supplemented with 50 mg/ml kanamycin and 10% glycerol and then stored at -80 C.
characteristics.characteristic: description
characteristics.text: Veraison
characteristics.characteristic: dev-stage
characteristics.text: TOP10 E.coli (Invitrogen)
characteristics.characteristic: lab-host
characteristics.text: Berry
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: deseeded berries
characteristics.characteristic: tissue-type
characteristics.text: Grape berry-specific cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR 2.1-TOPO vector (Invitrogen)
characteristics.characteristic: vector