characteristics.text: SAMN00150482
characteristics.characteristic: External Id
characteristics.text: LIBEST_019880
characteristics.characteristic: External Id
characteristics.text: British Columbia Genome Sciences Centre
characteristics.characteristic: INSDC center name
characteristics.text: 2006-06-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:48.523Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: LNCaP
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002071
characteristics.characteristic: cell-line
characteristics.text: LNCaP human prostate cancer cells (American Type Culture Collection; Bethesda, MD) were maintained in RPMI-1640 media (StemCell Technologies; Vancouver, BC) supplemented with 10% fetal bovine serum (FBS; StemCell Technologies) and incubated at 37C with 5% CO2. Cells at passage 38 were plated at a density of approximately 4x10^6 cells per T175 flask. Cells were serum-starved for 48 hours prior to treatment for 16 hours with 10 nM R1881 (PerkinElmer; Woodbridge, Canada). Cells were harvested and total RNA was extracted from the cells using TRIZOL Reagent (Invitrogen Life Technologies, Carlsbad, CA) following the manufacturer's instructions
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: prostate
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002367
characteristics.characteristic: tissue-type
characteristics.text: LNCAP + R1881 synthetic Androgen human prostate library
characteristics.characteristic: title