characteristics.text: LIBEST_023638
characteristics.characteristic: External Id
characteristics.text: SAMN00166531
characteristics.characteristic: External Id
characteristics.text: Oklahoma State University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:20.973Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from pooled guts and salivary glands of I. scapularis female ticks injected with subolesin dsRNA or injection buffer at 9 dpi (8 days of feeding) using TriReagent (Sigma) according to manufacturer's instructions. SSH was performed at Evrogen JCS (Moscow, Russia) as described previously (Naranjo et al., 2006; FEMS Immunol Med Microbiol 46: 298-312).
characteristics.characteristic: description
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: lab-host
characteristics.text: Ixodes scapularis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6945
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: pooled guts and salivary glands
characteristics.characteristic: tissue-type
characteristics.text: Subolesin dsRNA and saline injected Ixodes scapularis SSH Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAL-16
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector