characteristics.text: SAMN00150649
characteristics.characteristic: External Id
characteristics.text: LIBEST_020062
characteristics.characteristic: External Id
characteristics.text: Shandong Academy of Agricultural Science
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:55.617Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Luhua14
characteristics.characteristic: cultivar
characteristics.text: The peanut seeds from DAP (days after pegging) 20 to 60 were collected, and placed into liquid N2 immediately and stored in -80 C freezer. Total RNA was extracted from seeds by RNAgent kit (Promega). mRNA was isolated and purified from total RNA (Promega). Directional cDNA synthesis (using EcoRI, XhoI sites adapters) and library construction followed the protocol of Stratagene's pBluescript II cDNA library construction kit.
characteristics.characteristic: description
characteristics.text: DAP 20 to 60
characteristics.characteristic: dev-stage
characteristics.text: seeds
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000991
characteristics.characteristic: organ
characteristics.text: Arachis hypogaea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3818
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: seeds
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000991
characteristics.characteristic: tissue-type
characteristics.text: Peanut (Luhua14) seeds full length cDNA Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK
characteristics.characteristic: vector