characteristics.text: SAMN00156098
characteristics.characteristic: External Id
characteristics.text: LIBEST_001520
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-01-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:42.330Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag- 3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBK-CMV phagemid.
characteristics.characteristic: description
characteristics.text: E. coli XLOLR
characteristics.characteristic: lab-host
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: mixed male and female
characteristics.characteristic: sex
characteristics.text: 0 day fin regenerates
characteristics.characteristic: tissue-type
characteristics.text: zebrafish fin day0 regeneration
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBK-CMV
characteristics.characteristic: vector