characteristics.text: SAMN00150217
characteristics.characteristic: External Id
characteristics.text: LIBEST_016457
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2004-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:52.933Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: H11-11
characteristics.characteristic: cultivar
characteristics.text: Cultured cells [de Sa MM et al. (1992) Plant Physiology 98:728-737] were grown in media (45mL) supplemented with either 50uM salicylic acid, 50uM benzothiadiazole, 50uM methyl jasmonate, 20ug chitosan or 200uL of Pollacia radiosa extract. Cells were harvested after a 3 hour treatment, along with untreated control cells. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.
characteristics.characteristic: description
characteristics.text: E. coli DH10B T1 phage resistant cells
characteristics.characteristic: lab-host
characteristics.text: Populus trichocarpa x Populus deltoides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3695
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: PTxD-ICC-N-A-14
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector