characteristics.text: LIBEST_000361
characteristics.characteristic: External Id
characteristics.text: SAMN00154973
characteristics.characteristic: External Id
characteristics.text: Pohang University of Science and Technology
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.363Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ESTs were generated from the root cDNA library of Brassica napus. The cDNAs were primed with oligo(dT) primer. Before inserting the cDNAs into the pUC19 vector digested with SmaI, we deleted approximately 200 nucleotides from both 5' and 3' ends to remove some of the noncoding region of cDNAs. The SmaI site in pUC19 was destroyed by cloning.
characteristics.characteristic: description
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: SmaI
characteristics.characteristic: re_1
characteristics.text: SmaI
characteristics.characteristic: re_2
characteristics.text: L. cv Naehan
characteristics.characteristic: strain
characteristics.text: Deletion-treated
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC19
characteristics.characteristic: vector