characteristics.text: LIBEST_000268
characteristics.characteristic: External Id
characteristics.text: SAMN00154882
characteristics.characteristic: External Id
characteristics.text: Concordia University
characteristics.characteristic: INSDC center name
characteristics.text: 1995-03-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:07.330Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized with ZAP kit (Stratagene) using poly(A)+ RNA isolated from Aspergillus niger grown in both complete and minimal media. Synthesis was primed with oligo(dT) primer/XhoI-linker. EcoRI adaptors were later ligated to polished ends. EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pYES2 (Invitrogen Corp). This vector permits expression of our library in yeast.
characteristics.characteristic: description
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Aspergillus niger
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5061
characteristics.characteristic: organism
characteristics.text: Xho-I
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Aspergillus niger, pYES2 (XhoI-EcoRI)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pYES2
characteristics.characteristic: vector