characteristics.text: SAMN00164117
characteristics.characteristic: External Id
characteristics.text: LIBEST_009606
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research (TIGR; www.tigr.org)
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.180Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Bintje
characteristics.characteristic: cultivar
characteristics.text: Tissue supplied by Christian Bachem and Richard Visser (Department of Plant Breeding, Wageningen University, The Netherlands). The cSTA libraries will attempt to capture the induction and initiation/initial growth of the tuber in an in vitro system as described in Bachem et al. (Plant Journal, 1996). Small microtubers develop from axillary buds attached to stem explants when placed on a high sucrose medium (10%). Visible morphological changes occur synchronously at day five in the axillary buds. The first library, cSTA (1-20) consists of axillary buds harvested on days 1-3. This targets those genes involved in induction of the microtubers. The following libraries, cSTA (21-40) and cSTA (41-60), capture genes involved in tuber initiation and outgrowth. This library is noted as P3 in Tanksley lab notebooks.
characteristics.characteristic: description
characteristics.text: 7, 8 and 10 days
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Solanum tuberosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4113
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: axillary buds of stem explants; growing sink-tubers
characteristics.characteristic: tissue-type
characteristics.text: cSTE
characteristics.characteristic: title
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector