characteristics.text: SAMN00166683
characteristics.characteristic: External Id
characteristics.text: LIBEST_023725
characteristics.characteristic: External Id
characteristics.text: University of Milan
characteristics.characteristic: INSDC center name
characteristics.text: 2009-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:25.200Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Fiesta
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isolated from fruit flesh (a pool of early stage, middle stage and ripening stage fruits) according to Zeng and Yang (Plant Molecular Report 20:417a-417e, 2002). Poly(A)+mRNA extracted from fruit flesh was used to construct a subtractive library employing the PCR-Select cDNA Subtraction kit (Clontech) using fruit cDNA as tester and driver, to reduce redundancy. cDNA fragments were cloned into pGEM-T easy plasmid vector (Promega). The ligated cDNA fragments were transformed into E.coli JM109E host cells.
characteristics.characteristic: description
characteristics.text: JM109E cells
characteristics.characteristic: lab-host
characteristics.text: fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Malus domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3750
characteristics.characteristic: organism
characteristics.text: fruit flesh
characteristics.characteristic: tissue-type
characteristics.text: Md_fruit-fruit_subtractive_cDNA_library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy vector (Promega)
characteristics.characteristic: vector