characteristics.text: SAMN00004902
characteristics.characteristic: External Id
characteristics.text: DNA Data Bank of Japan
characteristics.characteristic: INSDC center name
characteristics.text: 2009-10-23T04:56:48.937Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:46:18.067Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS004111
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS004111
characteristics.characteristic: SRA accession
characteristics.text: tag_length:25-40bp,target_rna_length:10-26bp
characteristics.characteristic: THP-1 was stimulated by PMA and harvested after 24h, 48h, 72h, 96h replicate 1, 96h replicate 2
characteristics.text: The THP-1 cell line was sub-cloned and one clone (#5) was selected for ability to differentiate relatively homogeneously in response to PMA. THP-1.5 was used for all subsequent experiments. THP-1.5 cells were cultured in RPMI, 10% FBS, Penicillin/Streptomycin, 10mM HEPES, 1mM Sodium Pyruvate, 50uM 2-Mercaptoethanol, and treated with 30ng/ml PMA (Sigma) to differentiate them into macrophage-like cells. PMA-differentiated THP-1.5 were stimulated with 10ng/ml LPS (Salmonella Minnesota, chromatographically purified, Sigma) over a time-course of 24h, in presence of PMA. Total cell lysates were harvested in TRIzol reagent. Undifferentiated and unstimulated, PMA-differentiated cells were harvested in TRIzol reagent at the beginning of the LPS time-course. Total RNA was purified from TRIzol lysates according to manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Generic sample from Homo sapiens
characteristics.characteristic: title