characteristics.text: SAMN00163304
characteristics.characteristic: External Id
characteristics.text: LIBEST_008782
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-03-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:17.210Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams 82
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: The cDNA library was constructed from mRNA isolated from 9-11 day old seedlings that were induced for HR (hypersensitive response) by vacuum infiltrating plant tissue with Pseudomonas syringae pv. glycinea carrying the avrB gene (Genetics 141:1597-1604). Plant tissue (expanded unifoliate leaves) was collected at 2, 4, 8, 12, 24, 36, and 53 hrs after inoculation and their mRNA pooled equally for cDNA construction. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA insert is protected from XhoI digestion via methylation during first strand synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plant care, inoculations, and library construction were performed by Steve Clough (Lila Vodkin lab, University of Illinois).
characteristics.characteristic: description
characteristics.text: 9-11 day old
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: seedlings induced for HR (hypersensitive response)
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1074
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector