characteristics.text: SAMD00008966
characteristics.characteristic: External Id
characteristics.text: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus
characteristics.characteristic: INSDC center name
characteristics.text: 2012-09-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-11-12T03:29:14Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS001664
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS001664
characteristics.characteristic: SRA accession
characteristics.text: Nasutitermes takasagoensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_62960
characteristics.characteristic: organism
characteristics.text: Nasutitermes takasagoensis collected from Ishigaki Island were used. For construction of a primary cDNA library, the frontal glands were dissected from the heads of soldiers. The RNA may have been from frontal gland cells and around tissues such as muscle and fat bodies. First strand cDNA was synthesized using an oligo(dT)18 anchor primer that included an XhoI restriction site and an adaptor sequence with an EcoRI restriction site according to the Gubler-Hoffman method. The cDNA was ligated into a pBluescript II SK(+) vector digested with EcoRI and XhoI restriction enzymes. The vector was transfected into E. coli DH10B competent cells. The plasmid was purified by phenol-chloroform extraction and ethanol precipitation and then diluted in TE buffer. The primary cDNA library was amplified because the quantity was insufficient for cDNA library preparation for GS Junior sequencing. The primary cDNA library was transformed into E. coli HST08 Premium Competent Cells. The amplified plasmid was used as a sample DNA to prepare a shotgun library for GS Junior Titanium Series.
characteristics.characteristic: sample comment
characteristics.text: DRS001664
characteristics.characteristic: sample name
characteristics.text: cDNA library sequencing in the frontal gland of soldiers in Nasutitermes takasagoensis by 454 GS Junior pyrosequencing
characteristics.characteristic: title