characteristics.text: SAMN00165698
characteristics.characteristic: External Id
characteristics.text: LIBEST_023121
characteristics.characteristic: External Id
characteristics.text: University of Perugia
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:05.150Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Peripheral blood mononuclear cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_2000001
characteristics.characteristic: cell type
characteristics.text: This EST was obtained with the cDNA-AFLP method (Cappelli et al Res Vet Sci. 2005 Oct;79(2):105-12). Total RNA was extracted from two horses during a 140 km endurance ride. Blood samples were taken by jugular vein before ride and 0, 24 and 48 h after the competition. The restriction endonucleases EcoRI and MseI were used. Bands that resulted differentially expressed in the time course were isolated from the polyacrilamide gel, cloned in a plasmid vector (Invitrogen PCR4 TOPO) and sequenced.
characteristics.characteristic: description
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: lab-host
characteristics.text: Equus caballus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9796
characteristics.characteristic: organism
characteristics.text: Blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Endurance Horses PBMC Library
characteristics.characteristic: title
characteristics.text: Invitrogen PCR4 TOPO
characteristics.characteristic: vector