characteristics.text: SAMN00164543
characteristics.characteristic: External Id
characteristics.text: LIBEST_022308
characteristics.characteristic: External Id
characteristics.text: DOE Joint Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:40.840Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2.5kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pSPORT1 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
characteristics.characteristic: description
characteristics.text: early polyp
characteristics.characteristic: dev-stage
characteristics.text: Nematostella vectensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_45351
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: CACG Nematostella vectensis nemve mixed stage egg - early polyp M
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector