characteristics.text: SAMN00154958
characteristics.characteristic: External Id
characteristics.text: LIBEST_000346
characteristics.characteristic: External Id
characteristics.text: Yale University Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.043Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified from the ocular ciliary body of an adult female donor. A unidirectional cDNA library was constructed using lambda-Uni-ZAP XR vector (Stratagene, La Jolla, Ca) as described by Escribano et al. (J. Biochem. 1995). Using lambda DNA from the cDNA library as template and the set of primers indicated a 417-bp product was obtained, directly subcloned into the TA Cloning vector (pCR II) (Invitrogen Corp., San Diego, Ca), and further sequenced from the 5' and 3' ends. Forward Primer: ACGGTCGGATCCAGTGTTTCA Reverse Primer: AGGCGGGCTTGTTCTCCAT
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Human ocular ciliary body cDNA library 1
characteristics.characteristic: title
characteristics.text: TA
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005309
characteristics.characteristic: vector