characteristics.text: SAMN00014760
characteristics.characteristic: External Id
characteristics.text: Joint Genome Institute, U.S. Department of Energy
characteristics.characteristic: INSDC center name
characteristics.text: 2010-06-10T15:29:16.217Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-11-25T12:20:31.410Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS074580
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS074580
characteristics.characteristic: SRA accession
characteristics.text: Chlamydomonas reinhardtii pooled D66, cc1690 and cc125 sulfur and phosphorus starvation
characteristics.characteristic: description
characteristics.text: TAP medium depleted of phosphorous or sulfur; continuous light and shaking; 23 degC
characteristics.characteristic: in vivo conditions
characteristics.text: C. reinhardtii strains D66 cells were grown in modifed TAP medium with -S at 23 Celsius, 150 RPM shaking, and under continuous light at 90 µmol/m^2*s. RNA was isolated from cells collected at a cell density of 5.0x10^6 cells/ml. C. reinhardtii strains CC-1690 and CC-125 were grown in modifed TAP medium with -P at 25 Celsius, 200 RPM shaking, and under continuous light at 70 µmol/m^2*s. Cells were inoculated into TAP medium at a chlorophyll density of 2.5 mg/ml. RNA was collected from cells after 24 hours of growth. RNA samples were pooled in equal proportion for sequencing. Sample provided by Arthur Grossman.
characteristics.characteristic: library description
characteristics.text: RAPID
characteristics.characteristic: library type
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: D66,cc1690,cc125
characteristics.characteristic: strain
characteristics.text: Generic sample from Chlamydomonas reinhardtii
characteristics.characteristic: title