characteristics.text: LIBEST_009626
characteristics.characteristic: External Id
characteristics.text: SAMN00164137
characteristics.characteristic: External Id
characteristics.text: Centro de Biotecnologia
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.697Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Echinoccocus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. Prior to RNA extraction, protoscoleces were treated with pepsin (0.5 mg/ml in Hanks' solution pH 2.0, for 3 h at 37C). cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer for the recently characterised E. granulosus spliced leader sequence (Brehm et al, J Biol Chem 275: 38311-8, 2000). cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.
characteristics.characteristic: description
characteristics.text: larva (protoscolex)
characteristics.characteristic: dev-stage
characteristics.text: Echinococcus granulosus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6210
characteristics.characteristic: organism
characteristics.text: SalI (5'end)
characteristics.characteristic: re_1
characteristics.text: NotI (3'end)
characteristics.characteristic: re_2
characteristics.text: Echinococcus granulosus pepsin-treated protoscolex (trans-spliced)
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector