characteristics.text: SAMN00166320
characteristics.characteristic: External Id
characteristics.text: LIBEST_023350
characteristics.characteristic: External Id
characteristics.text: Guangxi University
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:11.950Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Equal amounts of mature taproot tissues by weight were sampled from two ends and central sections of the roots of 210-day-old cassava plants. Root tissues sampled from three plants with the same size were pooled and ground in liquid nitrogen to isolate the total RNA. About 5 ug of the resulting total RNA were employed for isolation of mRNA. Approximately 500 ng of mRNA were used to synthesis the first-strand cDNA which was then used to synthesis double-strand cDNA. The double-Strand cDNA was cloned into the NotI and SalI sites of pBluescript SK(+).
characteristics.characteristic: description
characteristics.text: 210-day-old plants grown in the experimental fields of Agricultural College, Guangxi University, China
characteristics.characteristic: dev-stage
characteristics.text: Manihot esculenta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3983
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: tissue-type
characteristics.text: Cassava root 210-day-old plants cDNA library
characteristics.characteristic: title
characteristics.text: pBluescript SK(+)
characteristics.characteristic: vector