characteristics.text: LIBEST_004072
characteristics.characteristic: External Id
characteristics.text: SAMN00158625
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:49.920Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-Oligo(dT) PCR-based library. Zeldia cDNA PCR products containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. This library was constructed by Merry Brook and Dr. Andrew Kloek at Divergence LLC. The strain PDL3 used in this work was provided by the Caenorhabditis Genetics Center at the University of Minnesota.
characteristics.characteristic: description
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: dev-stage
characteristics.text: Top10F'
characteristics.characteristic: lab-host
characteristics.text: Zeldia punctata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_49351
characteristics.characteristic: organism
characteristics.text: PDL3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007937
characteristics.characteristic: strain
characteristics.text: Kloek Brook Zeldia punctata SL1
characteristics.characteristic: title
characteristics.text: Ampicillin and Kanamycin resistant
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector