characteristics.text: LIBEST_021155
characteristics.characteristic: External Id
characteristics.text: SAMN00152898
characteristics.characteristic: External Id
characteristics.text: Stanford University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:10.990Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was prepared from a collection of California mussels that had been exposed to a variety of environmental challenges that included heat, cold, hypoxia, emersion, heavy metal, and salinity stress. The cDNA library was constructed using Cap-trapping methodology developed by RIKEN. Cap-trapped 1st strand cDNA was produced and hybridized with several biotinylated RNA drivers including: starting mRNA as the normalizing driver, and run-off transcripts from mini-libraries containing highly expressed genes, and previously sequenced cDNAs as subtracting drivers. cDNA that did not hybridize to the biotinylated driver was isolated, directionally-cloned into lambda FLC-I vector (from RIKEN), and plasmids excised using an in vivo liquid excision method.
characteristics.characteristic: description
characteristics.text: DH10B (T1 phage resistant)
characteristics.characteristic: lab-host
characteristics.text: Mytilus californianus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6549
characteristics.characteristic: organism
characteristics.text: GGCCAAATCGGCC
characteristics.characteristic: re_1
characteristics.text: GGCCCTTATGGCC
characteristics.characteristic: re_2
characteristics.text: Gill and adductor muscle
characteristics.characteristic: tissue-type
characteristics.text: SHGC-MUS
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pFLC-I
characteristics.characteristic: vector