characteristics.text: SAMN00154076
characteristics.characteristic: External Id
characteristics.text: LIBEST_015517
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2004-03-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:26.183Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library
characteristics.characteristic: description
characteristics.text: DH10B TonA
characteristics.characteristic: lab-host
characteristics.text: olfactory epithelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001997
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: DraIII
characteristics.characteristic: re_1
characteristics.text: DraIII
characteristics.characteristic: re_2
characteristics.text: olfactory epithelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001997
characteristics.characteristic: tissue-type
characteristics.text: NIH_ZGC_14
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pME18S-FL3
characteristics.characteristic: vector