characteristics.text: LIBEST_020539
characteristics.characteristic: External Id
characteristics.text: SAMN00152320
characteristics.characteristic: External Id
characteristics.text: Texas Tech University Health Sciences Center
characteristics.characteristic: INSDC center name
characteristics.text: 2006-10-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:52.517Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: peritubular cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_1001033
characteristics.characteristic: cell type
characteristics.text: Primary, unamplified cDNA libraries from 18-20 day mouse peritubular cells were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63428.
characteristics.characteristic: description
characteristics.text: 18-20 day
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli (XL2 Blue MRF')
characteristics.characteristic: lab-host
characteristics.text: testis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000473
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: CD-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005180
characteristics.characteristic: strain
characteristics.text: peritubular cells
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_1001033
characteristics.characteristic: tissue-type
characteristics.text: MacDonald peritubular cells
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Stratagene lambda Uni-Zap XR
characteristics.characteristic: vector