characteristics.text: LIBEST_000559
characteristics.characteristic: External Id
characteristics.text: SAMN00155164
characteristics.characteristic: External Id
characteristics.text: University of Berkeley
characteristics.characteristic: INSDC center name
characteristics.text: 1996-12-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:14.343Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA purified from rough endoplasmic reticulum-bound polysomes was used as a template. cDNA's directionally cloned at HinDIII and PstI in Bluescript SKt. Cloned into HinDIII, but the site was destroyed to add an adapter sequence. So ClaI is now the restriction enzyme at site I of vector. Primers - 5' universal, 3' m13-20 (reverse), 3' T3, 5' T7.
characteristics.characteristic: description
characteristics.text: 0 to 24 hours old embryo
characteristics.characteristic: dev-stage
characteristics.text: XL1 Blue MRF
characteristics.characteristic: lab-host
characteristics.text: embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: organ
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: ClaI
characteristics.characteristic: re_1
characteristics.text: PstI
characteristics.characteristic: re_2
characteristics.text: male and female
characteristics.characteristic: sex
characteristics.text: Drosophila Embryonic Polysomal in pBS skT
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: vector