characteristics.text: LIBEST_019721
characteristics.characteristic: External Id
characteristics.text: SAMN00150336
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:04.917Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:15:41.667Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Selection-12
characteristics.characteristic: cultivar
characteristics.text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit.The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry. Coffea arabica 'Selection-12' developed at Central Coffee Research Institute, Chikamagalur, India. Moisture-stressed leaf tissue obtained from Prof. Uday Kumar, Department of Plant physiology, University of Agricultural Sciences, Bangalore, india.
characteristics.characteristic: description
characteristics.text: Coffea arabica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13443
characteristics.characteristic: organism
characteristics.text: Moisture-stressed leaf tissue
characteristics.characteristic: tissue-type
characteristics.text: Leaf EST library of Coffea arabica cultivar S-12
characteristics.characteristic: title
characteristics.text: pMOSBlue Blunt Ended vector
characteristics.characteristic: vector