characteristics.text: LIBEST_009849
characteristics.characteristic: External Id
characteristics.text: SAMN00164352
characteristics.characteristic: External Id
characteristics.text: NONG WOO BIO CO.,LTD.
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.427Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PI257284
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_90524
characteristics.characteristic: cultivar
characteristics.text: The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.
characteristics.characteristic: description
characteristics.text: 7-8 weeks-old leaves
characteristics.characteristic: dev-stage
characteristics.text: Capsicum chinense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80379
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: PMMV-induced suppression subtractive cDNA library of Hot pepper
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector