characteristics.text: LIBGSS_000002
characteristics.characteristic: External Id
characteristics.text: SAMN00150918
characteristics.characteristic: External Id
characteristics.text: Howard Hughes Medical Institute
characteristics.characteristic: INSDC center name
characteristics.text: 1990-11-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:38:53.740Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Genomic DNA was isolated from a cloned population of bloodstream trypanosomes reexpressing the MVAT4 metacyclic variant surface glycoprotein (VSG). For the shotgun library construction, the DNA was mechanically sheared to give a tight size distribution, then blunt-ended with T4 DNA polymerase. Following dephosphorylation with Shrimp Alkaline Phosphatase, DNA fragments were cloned into the pCR-Script vector (Stratagene).
characteristics.characteristic: description
characteristics.text: Bloodstream form
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000134
characteristics.characteristic: dev-stage
characteristics.text: Trypanosoma brucei rhodesiense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_31286
characteristics.characteristic: organism
characteristics.text: Srf I
characteristics.characteristic: re_1
characteristics.text: MVAT4 sheared genomic library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR-Script Amp SK(+) (Stratagene)
characteristics.characteristic: vector