characteristics.text: LIBEST_005441
characteristics.characteristic: External Id
characteristics.text: SAMN00159979
characteristics.characteristic: External Id
characteristics.text: The University of Georgia, Department of Plant Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2000-07-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:31.293Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Two-week-old sorghum plants (BTX 623 cultivar) were infected with pathogen (isolate FRM42I of Colletotrichum graminicola, which is a sorghum isolate). RNA was prepared from infected leaves harvested from 45 seedlings 48 hours after inoculation. Note: young seedlings (2 weeks old) exhibit juvenile resistant reaction, which is an incompatible interaction. As they grow older (4 weeks or older), plants resume susceptiblity to anthracnose disease. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.
characteristics.characteristic: description
characteristics.text: Anthracnose-infected leaves from two-week-old sorghum plants 48 hr after inoculation
characteristics.characteristic: organ
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Pathogen induced 1 (PI1)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II from Lambda Zap II
characteristics.characteristic: vector