characteristics.text: LIBEST_019525
characteristics.characteristic: External Id
characteristics.text: SAMN00152421
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:20.817Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Chinese Spring addition line contain. chom 7E from T. elongata
characteristics.characteristic: cultivar
characteristics.text: Wheat plants (Chinese Spring and Chinese Spring addition line containing chromosome 7E from Thinopyrum elongatum) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis of the addition line were point-inoculated with Fg spores (10 ul of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used on Chinese Spring plants. Inoculated spikelets (with Fg or water) were harvested immediately after inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues of the addition line as the tester, subtracted with water-inoculated cDNA from Chinese Spring as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).
characteristics.characteristic: description
characteristics.text: anthesis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: dev-stage
characteristics.text: E. coli Sure Cells
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Chinese Spring addition line contain. chom 7E from T. elongata
characteristics.characteristic: strain
characteristics.text: spikelets
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009051
characteristics.characteristic: tissue-type
characteristics.text: Ta09_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGem-T easy
characteristics.characteristic: vector