characteristics.text: LIBEST_006776
characteristics.characteristic: External Id
characteristics.text: SAMN00161311
characteristics.characteristic: External Id
characteristics.text: Biomedical Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2000-10-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:16.130Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hemocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000387
characteristics.characteristic: cell type
characteristics.text: Total RNA was isolated from the hemocytes of unexposed and exposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using anchored oligo (dT11-CG) primers (Operon Technologies, Alameda, CA). Radioactively labelled second strand synthesis was carried out using one of twelve 10-mer arbitrary primers (Operon Technologies, Alameda, CA)in a PCR based amplification reaction. The labelled PCR products were separated using 8% polyacrylamide/8M urea gels and the differentially expressed fragments identified by autoradiography. The different bands of interest were sliced out from the gel and the DNA solubilized and precipitated. This DNA was reamplified using the anchored oligo dT-arbitrary primer combination and the product cloned into the plasmid vector pCRII/TOPO. The differential display products were all cloned in a similar fashion and then subjected to sequencing.
characteristics.characteristic: description
characteristics.text: Biomphalaria glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6526
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: BS-90
characteristics.characteristic: strain
characteristics.text: Biomphalaria glabrata (BS90) differential display
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII/TOPO
characteristics.characteristic: vector