characteristics.text: SAMN00155550
characteristics.characteristic: External Id
characteristics.text: LIBEST_000953
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 1997-08-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:27.160Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Brugia malayi is a lymphatic filarial nematode parasite of humans. mRNA was prepared from third stage larvae of Brugia malayi isolated from the peritoneal cavity of jirds six days after infection. The mRNA was converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 2 x 10E5 independent recombinants and average insert size was 900 base pairs. The library was constructed by Michelle Lizotte-Waniewski. The library is available from The Filarial Genome Project Resource Center: contact Dr. S.A. Williams, Clark Science Center, Smith College, Northampton, MA 01063 USA phone +1 413 585 3826 fax +1 413 585 3786 email genome@smith.edu.
characteristics.characteristic: description
characteristics.text: third stage larvae, six days after infection
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Eco R I (5' end)
characteristics.characteristic: re_1
characteristics.text: Xho I (3' end)
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: TRS Labs
characteristics.characteristic: strain
characteristics.text: Brugia malayi day 6 post-infection third stage larvae SAW96MLW-BmL3d6
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: lambdaZapII (UniZap XR)
characteristics.characteristic: vector