characteristics.text: LIBEST_012762
characteristics.characteristic: External Id
characteristics.text: SAMN00153975
characteristics.characteristic: External Id
characteristics.text: Michigan State University
characteristics.characteristic: INSDC center name
characteristics.text: 2003-03-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:05.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Latex was obtained from mature rubber plants (H. brasiliensis clone RRIM 600) growing at the Rubber Research Institute of Malaysia (RRIM), Selangor, Malaysia. We used the SMART strategy (Switching Mechanism At 5' end of the RNA Transcript; Clontech, palo alto, CA) to construct a high-content full-length cDNA library from the latex according to the manufacturers' instructions. This method is based on intrinsic terminal transferase activity of a reverse transcriptase (RT). The phage library was converted through mass excision to a phagemid library in the vector pTriplEX
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E.coli (BM52.8)
characteristics.characteristic: lab-host
characteristics.text: trunk (stem)
characteristics.characteristic: organ
characteristics.text: Hevea brasiliensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3981
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: Mull. Arg. (the Brazilian rubber tree), clone RRIM 600
characteristics.characteristic: strain
characteristics.text: laticifer
characteristics.characteristic: tissue-type
characteristics.text: Latex Lambda TriplEX cDNA Library
characteristics.characteristic: title
characteristics.text: pTriplEX2
characteristics.characteristic: vector