characteristics.text: LIBEST_008700
characteristics.characteristic: External Id
characteristics.text: SAMN00163226
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:14.370Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA oligo-dT primed using oligo 5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3' and directionally cloned into XhoI and BamHI sites using the 5' adaptor 5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3'. Size selection was done automatically by the cloning vector (lambda FLC-II, P. Carninci et al., unpublished). Library was amplified at the phage stage and normalized (RoT value = 5). This library was constructed using the CapTrapper method and Trehalose thermoactivated reverse transcriptase to enrich for full-length clones. Library constructed by Piero Carninci (RIKEN, Genome Science Laboratory, Japan: http://genome.rtc.riken.go.jp/) using egg RNA supplied by Marc Kirschner (Harvard Medical School, Dept. of Cell Biology). References: Methods Enzymol.303:19-44 and Genome Res. 10:1617-1630 Please contact Marc Kirschner (marc@hms.harvard.edu) for information on obtaining aliquots of this cDNA library.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: egg (unfertilized)
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002443
characteristics.characteristic: tissue-type
characteristics.text: RIKEN Xenopus egg
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pCS2G (custom)
characteristics.characteristic: vector