characteristics.text: LIBEST_020457
characteristics.characteristic: External Id
characteristics.text: SAMN00152186
characteristics.characteristic: External Id
characteristics.text: Cotton Research Institute of the Chinese Academy of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2006-10-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:49.363Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ms5ms6
characteristics.characteristic: cultivar
characteristics.text: EcoRI and MseI, cDNA-AFLP technique. Gossypium hirsutum plant male sterile and fertile anthers were collected after identification by the microscope in laboratory.RNA was extracted, and double-stranded cDNA was synthesized frome total RNA. The cDNA was digested with MseI and MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing two additional bases at the 3' end.
characteristics.characteristic: description
characteristics.text: DH5alpha
characteristics.characteristic: lab-host
characteristics.text: anther
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009066
characteristics.characteristic: organ
characteristics.text: Gossypium hirsutum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3635
characteristics.characteristic: organism
characteristics.text: MseI
characteristics.characteristic: re_1
characteristics.text: MseI
characteristics.characteristic: re_2
characteristics.text: GH_SF
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pTA2
characteristics.characteristic: vector