characteristics.text: SAMN00149633
characteristics.characteristic: External Id
characteristics.text: University of Washington
characteristics.characteristic: INSDC center name
characteristics.text: 2010-12-22T17:47:57.863Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-03-28T17:15:57.130Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS150416
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS150416
characteristics.characteristic: SRA accession
characteristics.text: Treated sample. The 3134 cell line was derived by transformation of C127, originally isolated from a mammary adenocacinoma tumor of the RIII mouse. Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2. Cells were transferred to 10% charcoal-dextran-treated, heat-inactivated fetal bovine serum for 48 hrs prior to hormone treatment (1hr with 100 nM dexamethasone)
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: 3134 cell line, plus Dex replicate 3
characteristics.characteristic: title