characteristics.text: SAMN00153916
characteristics.characteristic: External Id
characteristics.text: LIBEST_012103
characteristics.characteristic: External Id
characteristics.text: University of Minnesota
characteristics.characteristic: INSDC center name
characteristics.text: 2003-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:48.867Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ultra
characteristics.characteristic: cultivar
characteristics.text: Normal and proteoid roots of white lupin grown under P deficient conditions were harvested 7 and 10 DAE (days after emergence), and poly(A)+ RNA was isolated. The poly(A)+ RNA obtained from plants 7 and 10 DAE was combined in a 1:1 ratio and 7 ug total RNA was used for reconstruction of a proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene.
characteristics.characteristic: description
characteristics.text: 7 and 10 DAE
characteristics.characteristic: dev-stage
characteristics.text: -P normal and proteoid root (cluster root)
characteristics.characteristic: organ
characteristics.text: Lupinus albus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3870
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: -P normal and proteoid roots 7 and 10 DAE
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBLUESCRIPT SK(+/-)
characteristics.characteristic: vector