characteristics.text: SAMN00156456
characteristics.characteristic: External Id
characteristics.text: LIBEST_001892
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-07-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:53.780Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from immature seed coats (200-300 mgs) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restriction site. Sal I linker adapters were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells (Gibco BRL). This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.
characteristics.characteristic: description
characteristics.text: DH10B (Gibco BRL)
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Sal I
characteristics.characteristic: re_2
characteristics.text: immature seed coats of greenhouse grown plants
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1019
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1 (Life Technologies)
characteristics.characteristic: vector