characteristics.text: 100
characteristics.characteristic: Congo Red
characteristics.text: SAMN00017031
characteristics.characteristic: External Id
characteristics.text: DOE JGI
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-16T19:27:32.753Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:57:22.273Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS085004
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS085004
characteristics.characteristic: SRA accession
characteristics.text: cell wall damage; congo red
characteristics.characteristic: assay
characteristics.text: YPD
characteristics.characteristic: culture media
characteristics.text: For the cell wall damage experiments, a saturated overnight culture of strain SC5314 was diluted into 600 mls of YPD medium to an OD600 of 0.1 and allowed to grow at 30*C until the culture reached an OD600 of ~1.0. At this point the culture was split into 2- 250ml cultures in separate 1L flasks. To one of the cultures, a stock solution of Congo Red (Sigma Aldrich), dissolved in water, was added to a final concentration of 100 micrograms/microliter. An equal volume of water was added to the other 250 ml culture as an untreated control. After 2 hours of treatment at 30*C, the cells were harvested by centrifugation and immediately stored at -80*C for later RNA isolation.
characteristics.characteristic: description
characteristics.text: Candida albicans SC5314
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_237561
characteristics.characteristic: organism
characteristics.text: SC5314
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006481
characteristics.characteristic: strain
characteristics.text: C. albicans SC5314 grown in cell wall damaging environment (Congo Red)
characteristics.characteristic: title