characteristics.text: LIBEST_005661
characteristics.characteristic: External Id
characteristics.text: SAMN00160197
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 2000-09-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:39.137Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4 to 4.5 kb. There are less than 0.5% clones with multiple inserts. Since each cDNA has an EcoRI site at its 5' end and an XhoI site at the 3' end, these clones can be easily identified. One should be suspicious of any clone which gives 3 or more bands in an EcoRI-XhoI double digest AND has an internal XhoI site. We usually do not further characterize any such clones unless the cDNA is known to give multiple bands in an EcoRI-XhoI digest. Microplate status: 500,000 unamplified cDNAs were mass excised (pBluescript SK-) in Xl1-Blue using ExAssist phage. The resulting single-stranded phagemids were used to infect Top10F'. Clones were picked into freezing medium (per liter 15 g tryptone, 10g yeast extract, 5g NaCl, 36 mM K2HPO4, 13.2 mM KH2PO4, 1.7 mM Na-citrate, 0.4 mM MgSO4*7 H2O, 6.8 mM (NH4)2SO4, 4% w/v glycerol) and grown for 24 hours. Original library contruction by Bruce Blumberg (Blumberg et al., 1991 Science 253, 194-196; Hawlet et al., 1995, Genes Dev. 9, 2923-2935). Note: This is a Xenopus Gene Collection (XGC) library
characteristics.characteristic: description
characteristics.text: Top-10 F'
characteristics.characteristic: lab-host
characteristics.text: Xenopus laevis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8355
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: unfertilized egg
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002443
characteristics.characteristic: tissue-type
characteristics.text: Xenopus laevis unfertilized egg cDNA library
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK-
characteristics.characteristic: vector