characteristics.text: SAMN00155570
characteristics.characteristic: External Id
characteristics.text: LIBEST_000978
characteristics.characteristic: External Id
characteristics.text: Queensland Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 1997-08-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:27.750Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Eggs of Schistosoma japonicum (Philippine strain) were purified from livers of rabbits about 50 days after infection with cercariae at the College of Public Health, University of The Philippines, Manila, The Philippines. Subsequently, at The Queensland Institute of Medical Research, Brisbane, Australia, messenger RNA was isolated from lysates of the S. japonicum eggs by oligo dT chromatography, first strand cDNA synthesis from the mRNA was primed with an oligo dT-XhoI primer and synthesized with M-MLV reverse transcriptase, and second strand synthesis was accomplished with RNAse H and T4 DNA polymerase. The double stranded cDNA was ligated into the phagemid vector UNI ZAP XR (Stratagene), and the ligation products packaged using reagents from Stratagene. The library was amplified using BB4 strain E. coli bacteria. The library was constructed by Sharon Day, Rolando Tan, Wilfred Tiu, and Paul Brindley who consider that it contains sequences expressed by immature and mature eggs of S. japonicum. The library is named 'PME-SD.96'
characteristics.characteristic: description
characteristics.text: Egg
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000369
characteristics.characteristic: dev-stage
characteristics.text: Rabbit
characteristics.characteristic: lab-host
characteristics.text: Schistosoma japonicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6182
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: Mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Philippine strain
characteristics.characteristic: strain
characteristics.text: Whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: PME-SD.96
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP-II XR (Stratagene)
characteristics.characteristic: vector