characteristics.text: SAMN00155988
characteristics.characteristic: External Id
characteristics.text: LIBEST_001409
characteristics.characteristic: External Id
characteristics.text: Washington University School of MedicineP
characteristics.characteristic: INSDC center name
characteristics.text: 1998-11-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:39.737Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: colon cancer cell line MAC13
characteristics.characteristic: cell-line
characteristics.text: Double-stranded cDNA was prepared from cell line MAC13 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
characteristics.characteristic: description
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: colon cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000365
characteristics.characteristic: tissue-type
characteristics.text: Schiller mouse MAC13
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescript SK- (Stratagene)
characteristics.characteristic: vector