characteristics.text: LIBEST_019521
characteristics.characteristic: External Id
characteristics.text: SAMN00150158
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:20.710Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Roblin
characteristics.characteristic: cultivar
characteristics.text: Wheat plants (cv Roblin) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis were point-inoculated with Fg spores (10 µl of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used as an experimental control on separate plants. Inoculated plants were misted for 10- second intervals every 15 min for 2 days. Inoculated spikelets were harvested 4 days post inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues as the tester, subtracted with water-inoculated cDNA as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).
characteristics.characteristic: description
characteristics.text: 4 days after anthesis
characteristics.characteristic: dev-stage
characteristics.text: E. coli Sure Cells
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: spikelets
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009051
characteristics.characteristic: tissue-type
characteristics.text: Ta07_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGem-T easy
characteristics.characteristic: vector