characteristics.text: LIBEST_021027
characteristics.characteristic: External Id
characteristics.text: SAMN00152785
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:07.023Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: H898
characteristics.characteristic: cultivar
characteristics.text: New shoot growth was harvested from 5 mature trees. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen).
characteristics.characteristic: description
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Picea sitchensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3332
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of the cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of the cDNA)
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Shoot
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000992
characteristics.characteristic: tissue-type
characteristics.text: SS-S-25
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector