characteristics.text: SAMN00163322
characteristics.characteristic: External Id
characteristics.text: LIBEST_008802
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-04-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:17.730Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams 82
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: The cDNA library was constructed from mRNA isolated from 11 day old seedlings treated with that were treated with 2 ugs/ml of a crude glucan elicitor preparation isolated from the mycelial walls of Phytophthora sojae. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plant material was provided by Michael G. Hahn (Complex Carbohydrate Research Center, University of Georgia) and the library was constructed by Anu Khanna (Lila Vodkin lab, University of Illinois).
characteristics.characteristic: description
characteristics.text: 11 day old seedlings
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: wounded cotyledons
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1076
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector