characteristics.text: SAMN00160115
characteristics.characteristic: External Id
characteristics.text: LIBEST_005577
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:36.770Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-Oligo(dT) PCR-based library. Heterodera glycines cDNA PCR products of size approximately 600-1200 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-T PO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University
characteristics.characteristic: description
characteristics.text: enriched for 2nd stage juveniles
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Heterodera glycines
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_51029
characteristics.characteristic: organism
characteristics.text: Heterodera glycines J2 SL1 Topo1 Kloek Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: Ampicillin and Kanamycin resistant
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO
characteristics.characteristic: vector