characteristics.text: LIBEST_019406
characteristics.characteristic: External Id
characteristics.text: SAMN00165146
characteristics.characteristic: External Id
characteristics.text: University of Wisconsin
characteristics.characteristic: INSDC center name
characteristics.text: 2006-10-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:17.583Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nipponbare
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007587
characteristics.characteristic: cultivar
characteristics.text: The library contains RTPCR products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using a mix of three CentO-specific primers designed from a forward strand (CentO_U 5' TCA TGT TTT GGT GCT TTT TG 3', CentO_F1 5' CAA TAT GTC CAA AAA NCA TGT TT 3', and CentO_F2 5' CGA ACG CAC CCA ATA CAN T 3'). The cDNA was used as a template for PCR amplification using CentO_U (5' TCA TGT TTT GGT GCT TTT TG 3') and CentO_L+ (5' GNT TTT TGG ACA TAT TGG AGT G 3') primers.
characteristics.characteristic: description
characteristics.text: flowering
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: dev-stage
characteristics.text: panicle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009049
characteristics.characteristic: organ
characteristics.text: Oryza sativa Japonica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: organism
characteristics.text: LIB_ID:66
characteristics.characteristic: title