characteristics.text: SAMN00154922
characteristics.characteristic: External Id
characteristics.text: LIBEST_000309
characteristics.characteristic: External Id
characteristics.text: University of Southern California
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:08.317Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Poly(A)+ RNA was purified from a 97% pure population of osteoclasts prepared from the long bones of 10 day old rabbits. First strand cDNA was synthesized by priming with an oligo(dT)-NotI anchor-primer and second strand cDNA was synthesized by replacement synthesis as described by Gubler and Hoffman (Gene 25:283, 1983). Following the addition of SalI adapters and NotI digestion, the cDNA was cloned between the SalI (5o) and NotI (3o) sites of the pSPORT1 (BRL) plasmid vector.
characteristics.characteristic: description
characteristics.text: E. coli DH12S
characteristics.characteristic: lab-host
characteristics.text: Oryctolagus cuniculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9986
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: New Zealand White
characteristics.characteristic: strain
characteristics.text: Rabbit Osteoclast, Dennis Sakai
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector