characteristics.text: SAMN00155463
characteristics.characteristic: External Id
characteristics.text: LIBEST_000864
characteristics.characteristic: External Id
characteristics.text: Yale University
characteristics.characteristic: INSDC center name
characteristics.text: 1997-05-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:23.803Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Burns et al., Genes Dev. 8, 1087-1105 (1994). A yeast genomic DNA library was prepared in the vector pHSS6-Sal, and subjected to transposon mutagenesis with mTn-LEU2/lacZ. This mini-transposon carries lacZ sequences that lack an initiation codon; expression of lacZ is only provided by in-frame fusion to yeast coding sequence. The yeast genomic DNA carrying the mTn-LEU2/lacZ insertion was released from pHSS6-Sal by digestion with NotI, and transplaced back onto the yeast chromosome in diploid strain Y800. Yeast colonies expressing lacZ were screened using a color assay for beta-galactosidase activity. Using either yIP5 (Burns et al.) or pRSQ (Ross-Macdonald et al., Methods in Molecular and Cellular Biology 5, 298-308. 1995), a plasmid containing the fusion junction between genomic DNA and the lacZ end of mTn-LEU2/lacZ was recovered from each individual yeast transformant that expressed lacZ activity. These recovered plasmids comprise 'The blue guys library'. The fusion junction was then sequenced using a primer in the mTn's lacZ region. This sequence data was analyzed to identify the expressed ORF upstream of the fusion.
characteristics.characteristic: description
characteristics.text: E.coli
characteristics.characteristic: lab-host
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Y800
characteristics.characteristic: strain
characteristics.text: Yeast::mTn-lacZ/LEU2 library
characteristics.characteristic: title
characteristics.text: hybrid
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000601
characteristics.characteristic: vector