characteristics.text: LIBEST_023705
characteristics.characteristic: External Id
characteristics.text: SAMN00166664
characteristics.characteristic: External Id
characteristics.text: NRC Plant Biotechnology Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:23.117Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nugget
characteristics.characteristic: cultivar
characteristics.text: The library HLUTR3CH was constructed from RNA isolated from lupulin glands from the variety 'Nugget'. Hop cones were collected near Baasdorf, Germany in August 2005. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). The library was constructed using a cDNA library synthesis kit (Stratagene) with cDNAs ligated into the plasmid vector pBluescript SK+. HLUTR3CH was normalized using a hydroxyapatite-based method (C0t 2.5 and 5) (Bonaldo et al. (1996) Genome Res. 6, 791).
characteristics.characteristic: description
characteristics.text: Trichomes isolated from mid to ripe stage hop cones
characteristics.characteristic: dev-stage
characteristics.text: Humulus lupulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3486
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Trichome (lupulin gland)
characteristics.characteristic: tissue-type
characteristics.text: HLUTR3CH
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: pBluescript SK+
characteristics.characteristic: vector