characteristics.text: SAMN00153409
characteristics.characteristic: External Id
characteristics.text: LIBEST_021748
characteristics.characteristic: External Id
characteristics.text: J. Craig Venter Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:26.317Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Arena
characteristics.characteristic: cultivar
characteristics.text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones.
characteristics.characteristic: description
characteristics.text: Raphanus sativus var. oleiformis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_463157
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: whole seedling (with 1 set of true leaves), buds, and anthers
characteristics.characteristic: tissue-type
characteristics.text: RS1(AR)
characteristics.characteristic: title
characteristics.text: pDNR-LIB(Clontech)
characteristics.characteristic: vector