characteristics.text: SAMN00150659
characteristics.characteristic: External Id
characteristics.text: LIBEST_020077
characteristics.characteristic: External Id
characteristics.text: Missouri State University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:37.183Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cabernet Sauvignon
characteristics.characteristic: cultivar
characteristics.text: Powdery mildew-infected Vitis vinifera 'Cabernet sauvignon' leaves were harvested at 0, 4, 8, 12, 24, and 48 h post-inoculation as tissue source for SSH library. Total RNA was extracted individually for each time point. Equimolar amounts of RNA for the 0, 4, and 8 h (early-infection stage) samples and for the 12, 24, and 48 h (late-infection stage) samples were pooled and reverse transcribed to cDNA using BD SMART PCR cDNA synthesis Kit (Clontech, Paolo Alto, California). SSH library was constructed with PCR select cDNA Subtraction Kit (Clontech) using early- and late-infection stage cDNA as tester and driver, respectively. PCR-amplified inserts were sequenced with T7 or SP6 vector-specific primers.
characteristics.characteristic: description
characteristics.text: young leaf
characteristics.characteristic: dev-stage
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: Vitis vinifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29760
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: leaf blade
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0020039
characteristics.characteristic: tissue-type
characteristics.text: Early Infection Stage Subtractive Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-5Zf(+)
characteristics.characteristic: vector