characteristics.text: LIBEST_018165
characteristics.characteristic: External Id
characteristics.text: SAMN00152291
characteristics.characteristic: External Id
characteristics.text: Institute of Plant Biology, National Taiwan University
characteristics.characteristic: INSDC center name
characteristics.text: 2005-09-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:46.233Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hawaii7996
characteristics.characteristic: cultivar
characteristics.text: 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled Rs-inoculated specimen from pooled control specimen using CLONTECH PCR-Select cDNA Subtraction kit.
characteristics.characteristic: description
characteristics.text: Three weeks old
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: root and collar
characteristics.characteristic: tissue-type
characteristics.text: Reverse subtractive cDNA library of tomato inoculated with Ralstonia solanacearum
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-Teasy
characteristics.characteristic: vector