characteristics.text: LIBEST_008825
characteristics.characteristic: External Id
characteristics.text: SAMN00163345
characteristics.characteristic: External Id
characteristics.text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2001-04-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:18.317Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Brevor
characteristics.characteristic: cultivar
characteristics.text: Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
characteristics.characteristic: description
characteristics.text: Mature seed
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007632
characteristics.characteristic: dev-stage
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Seed embryo
characteristics.characteristic: tissue-type
characteristics.text: Wheat dormant embryo cDNA library
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR, excised phagemid
characteristics.characteristic: vector