characteristics.text: LIBEST_020722
characteristics.characteristic: External Id
characteristics.text: SAMN00152505
characteristics.characteristic: External Id
characteristics.text: USDA-ARS-NAA Foreign Disease-Weed Science Research Unit
characteristics.characteristic: INSDC center name
characteristics.text: 2007-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:58.100Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Phakopsora pachyrhizi TW72-1 urediniospores (collected from infected soybean plants, frozen in liquid nitrogen and kept at -80C). 1. Total RNA was isolated from urediniospores of P. pachyrhizi isolate TW 72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a NotI primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.
characteristics.characteristic: description
characteristics.text: Urediniospores
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Phakopsora pachyrhizi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_170000
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Taiwan 72-1
characteristics.characteristic: strain
characteristics.text: CZAA Phakopsora pachyrhizi TW72-1 urediniospores
characteristics.characteristic: title
characteristics.text: pSPORT1
characteristics.characteristic: vector