characteristics.text: SAMN00120060
characteristics.characteristic: External Id
characteristics.text: Oregon State University
characteristics.characteristic: INSDC center name
characteristics.text: 2010-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-03-13T15:28:53.077Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS120983
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS120983
characteristics.characteristic: SRA accession
characteristics.text: National Clonal Germplasm Repository PI 551572
characteristics.characteristic: bio-material
characteristics.text: Genomic DNA was isolated from from young leaves 'DNAeasy Mini Plant RNA kit' (Qiagen) according to the manufacturer's protocol. Genomic DNA library was prepared according to modified standard Illumina protocol (http://www.illumina.com/). Briefly, 7 ug of genomic DNA in a 50 uL of TE buffer (10 mM Tris, 1 mM EDTA; pH 8.0) was mixed with 750 uL of nebulization buffer [25% (v/v) glycerol in TE buffer] and sheared for 7 min. in a nebulizer (Invitrogen) using compressed nitrogen at 36 psi. Before ligation with Illumina adapters the fragmented DNA was purified using 'QIAquick PCR Purification Kit' (Qiagen). Illumina library size was in 300-350 bp range.
characteristics.characteristic: description
characteristics.text: Fragaria vesca subsp. vesca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_101020
characteristics.characteristic: organism
characteristics.text: line Hawaii 4
characteristics.characteristic: orgmod_note
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue type
characteristics.text: Fragaria vesca subsp. vesca H4x4 leaf gDNA
characteristics.characteristic: title