characteristics.text: LIBEST_017827
characteristics.characteristic: External Id
characteristics.text: SAMN00149859
characteristics.characteristic: External Id
characteristics.text: Texas A&M University
characteristics.characteristic: INSDC center name
characteristics.text: 2005-10-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:36.010Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Texas Maker-1 (TM-1)
characteristics.characteristic: cultivar
characteristics.text: G. hirsutum L. Texas Marker-1 (TM-1) plants were grown in a greenhouse at the Texas A&M University and in the field at the USDA-ARS, New Orleans. The bolls were tagged and harvested at 3 days before anthesis (-3DPA), the day of anthesis (0 DPA), and 3 days post anthesis (3DPA) from a pool of ~20 plants. Total RNA was extracted from immature ovules or fiber-bearing ovules. An equal amount of total RNA from three samples was mixed and sent to Invitrogen Corp. (Carsbad, California) for full-length cDNA library construction. Messenger RNA was isolated from the total RNA using a filter syringe containing oligo (dT). The first-strand cDNA was synthesized from 15 ug mRNA using SuperScriptTMIII reverse transcriptase, and the second-strand cDNA was synthesized using E. coli RNase H, DNA polymerase I, and DNA ligase. The double-stranded cDNA was blunt-ended using T4 DNA polymerase, digested with NotI, size-selected using agarose gel electrophoresis, and directionally cloned into the NotI-EcoRV sites of the pCMV-SPORT6.1. The ligated products were transformed using ELECTROMAXTMDH10BT1 cells. The library contained 4.2x106 cfu with ~100% of colony growth rate. It is estimated that over 60% clones contained full-length cDNA inserts with an average insert size of 1.53-kb. A total of 51,072 colonies were arrayed in duplicate sets each with 133 384-well plates for sequencing and storage, respectively.
characteristics.characteristic: description
characteristics.text: Immature ovules (-3 to 3 days post anthesis, DPA) with or without fibers
characteristics.characteristic: dev-stage
characteristics.text: Gossypium hirsutum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3635
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Immature ovules
characteristics.characteristic: tissue-type
characteristics.text: GH_TMO
characteristics.characteristic: title
characteristics.text: pCMV-SPORT6.1
characteristics.characteristic: vector