characteristics.text: SAMN00154018
characteristics.characteristic: External Id
characteristics.text: LIBEST_014195
characteristics.characteristic: External Id
characteristics.text: Stanford University
characteristics.characteristic: INSDC center name
characteristics.text: 2003-08-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:48.237Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B73
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007022
characteristics.characteristic: cultivar
characteristics.text: Maize Gene Discovery Project contracted with Invitrogen to produce a normalized, full length library in a pSport vector. This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. Details of the vector and sequencing primers are available at ZmDB in the EST library description tables. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each of the 12 tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources. This step effected a 20X to 80X reduction in common transcript types. Tissues prepared: 1. just emerging silks; 2. inner husks from ears of sample #1; 3. 20 dap aleurone; 4. immature tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day old seedlings; all leaves with an expanded or partially expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 cm long root tips from 15 day old seedlings; 10. 10 dap whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap endosperm and embryo. All of the sequenced clones in project 3530 will be archived at the University of Arizona along with the Unigene clones from the Maize Gene Discovery EST sequencing projects. Clones can be ordered through the ZmDB web site or directly from the University of Arizona (http://www.genome.arizona.edu/orders/). High density filters containing over 18,000 clones can also be ordered from the University of Arizona.
characteristics.characteristic: description
characteristics.text: varies by tissue
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: silks, husks, ears, pollen, shoot tips, leaf, root tips, whole seed, embryo
characteristics.characteristic: organ
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRV
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: multiple
characteristics.characteristic: tissue-type
characteristics.text: 3530 - Full length cDNA library created by Invitrogen from multiple tissues
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pCMV-SPORT 6.1
characteristics.characteristic: vector