characteristics.text: LIBEST_023278
characteristics.characteristic: External Id
characteristics.text: SAMN00165851
characteristics.characteristic: External Id
characteristics.text: MPI for Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:09.973Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made from a suppression subtractive hybridisation (SSH): mRNA extracted from pool of whole larvae at day10 post hatching subtracted by mRNA extracted from pool of juveniles (day50 post hatching). Collected larvae were frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purifications were performed using oligotex mRNA midi kit (QIAGEN). Library construction was performed with PCR-select cDNA Substraction Kit (Clontech) and the Advantage cDNA polymerase Mix (Clontech). PCR products were inserted in pCR(R) II-TOPO(R) (Invitrogen) and cloned in XL 10-Gold(R) Kanr Ultracompetent Cells (Stratagene). Library sequencing was performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 2006. collected_by: Maria Darias Caceres and David Mazurais
characteristics.characteristic: description
characteristics.text: larvae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000069
characteristics.characteristic: dev-stage
characteristics.text: Dicentrarchus labrax
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13489
characteristics.characteristic: organism
characteristics.text: undifferentiated
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000621
characteristics.characteristic: sex
characteristics.text: whole larvae
characteristics.characteristic: tissue-type
characteristics.text: mge014
characteristics.characteristic: title