characteristics.text: LIBEST_005364
characteristics.characteristic: External Id
characteristics.text: SAMN00159904
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-06-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:28.110Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams 82
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from mRNA isolated from hypocotyl and plumule tissues of seeds germinated for three days of the cultivar Williams 82. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a NotI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestions with EcoRI and NotI. The cDNA fragments were directionally cloned into the EcoRI-NotI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Hypocotyl and Plumule, germinating seeds
characteristics.characteristic: tissue-type
characteristics.text: Gm-c1040
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pT7T3Pac (Pharmacia)
characteristics.characteristic: vector