characteristics.text: LIBEST_012636
characteristics.characteristic: External Id
characteristics.text: SAMN00152247
characteristics.characteristic: External Id
characteristics.text: Purdue University
characteristics.characteristic: INSDC center name
characteristics.text: 2003-02-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:02.777Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 70320
characteristics.characteristic: cultivar
characteristics.text: cDNAs made from fruit mRNAs of a thin-juice breeding line (70320) were subtracted by cDNAs made from fruit mRNAs of a thick-juice breeding line (70620) according to the manufacture's protocol of PCR-select cDNA subtraction protocol (Clonetech manual #PT1117-1). The resultant PCR amplified products were ligated into a TA vector pCR 2.1 (Invitrogen) and electroporated into E.coli DH5a.
characteristics.characteristic: description
characteristics.text: turning stage fruit
characteristics.characteristic: dev-stage
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: EcoR I
characteristics.characteristic: re_2
characteristics.text: pericarp
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009084
characteristics.characteristic: tissue-type
characteristics.text: tomato thin-juice fruit subtracted by cDNAs of thick-juice fruit
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector