characteristics.text: LIBEST_023416
characteristics.characteristic: External Id
characteristics.text: SAMN00165986
characteristics.characteristic: External Id
characteristics.text: MPI for Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:14.520Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: collection_date: 10-Oct-2004; collected_by: Juan Fuentes and Carla Viegas; isolation_source: aquaculture fish. Total RNA was extracted from 9 specimens of both sex (4 females and 5 males) and weight range 350 g to 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library contruction and sequencing were performed at the MPI of Molecular Genetics Berlin.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Sparus aurata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8175
characteristics.characteristic: organism
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: ultimobranchial gland tissue
characteristics.characteristic: tissue-type
characteristics.text: cDN16
characteristics.characteristic: title