characteristics.text: LIBEST_010250
characteristics.characteristic: External Id
characteristics.text: SAMN00153770
characteristics.characteristic: External Id
characteristics.text: University of Colorado at Boulder
characteristics.characteristic: INSDC center name
characteristics.text: 2002-02-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:59.247Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-PCR-TOPO based library. Ancylostoma ceylanicum cDNA were PCR amplified, using SL1-EcoRI primer on the 5' end and oligo(dT)-XhoI on 3' end, were gel fractionated, and non-directionally cloned into pCR-TOPO-XL (Invitrogen), following TOPO-XL TA cloning protocol. The cDNA insert can be excissed with EcoRI. The library was constructed by Adriana Magalska and dr. Vadim Kapulkin at Department of Infectious Diseasaes, Microbiology and Immunology of Veterinary Faculty at the Warsaw Agricultural University. The Ancylostoma ceylanicum strain used in this work was originally provided by Dr. Jerzy Behnke at the University of Nottingham.
characteristics.characteristic: description
characteristics.text: adult, mixed sex
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Ancylostoma ceylanicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_53326
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: female and male
characteristics.characteristic: sex
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Vadim Kapulkin Ancylostoma ceylanicum adult SL1-PCR sub-library
characteristics.characteristic: title
characteristics.text: pCR-TOPO-XL
characteristics.characteristic: vector