characteristics.text: LIBGSS_000016
characteristics.characteristic: External Id
characteristics.text: SAMN00150926
characteristics.characteristic: External Id
characteristics.text: The University of Texas Health Science Center
characteristics.characteristic: INSDC center name
characteristics.text: 1997-04-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-04-18T21:04:02.978Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Genomic DNA from Rhodobacter sphaeroides was prepared and partially digested with Sau3AI. Size selected (20kb) fragments were subcloned into the BglII site of the cosmid vector pLA2917 (Allen, L. N. and R. S. Hanson. 1985. Journal of Bacteriology, 161:955-962. The library was then ordered around chromosome II of Rhodobacter sphaeroides (Choudhary et al., 1994. Journal of Bacteriology, 176:7694-7702). The cosmids were then digested with a variety of restriction enzymes (BamHI, EcoRI, BglII, PstI, EcoRV, NotI and DNaseI) and restriction fragments subcloned into the respective multiple cloning site sites of pBluescript SK (-). Note BglII fragments were subcloned into the pBluescript BamHI site. DNaseI fragments were subcloned into the EcoRV site. All subclones were transformed into E. coli XL1Blue MRF'. All fragments were then sequenced and the sequences where possible were assembled using the GCG program GELASSEMBLE.
characteristics.characteristic: description
characteristics.text: E. coli S17-1
characteristics.characteristic: lab-host
characteristics.text: Cereibacter sphaeroides
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1063
characteristics.characteristic: organism
characteristics.text: 2.4.1T
characteristics.characteristic: strain
characteristics.text: Rhodobacter sphaeroides 2.4.1 genomic DNA library
characteristics.characteristic: title
characteristics.text: type strain of Rhodobacter sphaeroides
characteristics.characteristic: type material
characteristics.text: Cosmid
characteristics.characteristic: v_type
characteristics.text: pLA2917
characteristics.characteristic: vector