characteristics.text: LIBEST_021377
characteristics.characteristic: External Id
characteristics.text: SAMN00153098
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:16.887Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nicotiana benthamiana plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from stems of 5 week old N. benthamiana plants taken from the top half of the shoot. The stems were placed in 50 ml disposable falcon tubes and trichomes were abraded by vortexing for 20 s after freezing in liquid nitrogen. RNA was isolated using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.
characteristics.characteristic: description
characteristics.text: Apical stem internodes of 5-week old plants.
characteristics.characteristic: dev-stage
characteristics.text: Nicotiana benthamiana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4100
characteristics.characteristic: organism
characteristics.text: Sfi I A (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: Sfi I B (GGCCGCCTCGGCC)
characteristics.characteristic: re_2
characteristics.text: Isolated stem trichomes
characteristics.characteristic: tissue-type
characteristics.text: Nicotiana benthamiana trichomes
characteristics.characteristic: title
characteristics.text: pDNR_lib (Clontech)
characteristics.characteristic: vector