characteristics.text: LIBEST_020696
characteristics.characteristic: External Id
characteristics.text: SAMN00154382
characteristics.characteristic: External Id
characteristics.text: Children's National Medical Center
characteristics.characteristic: INSDC center name
characteristics.text: 2007-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:57.387Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Muscle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002385
characteristics.characteristic: cell type
characteristics.text: Torpedo californica electric organ was grinded in liquid nitrogen and total RNA was isolated. Seven milligrams of total RNA was sent to Amplicon Express (Pullman, WA) for the construction of cDNA library (5.16 x 10^6 cfu/ml primary colonies). Cloning sites used for insertion were Eco RV (GGAATTC/GATATCAAG) and Not I (site of poly A tail [CCGCGGTGG/CGGCCGCT]). Colonies were gridded onto 384 well plates containing 15% glycerol. Clones were sequenced from the 5' end using the T3 primer (5'ATTAACCCTCACTAAAGGGA 3'). Clones were either sequenced by first plasmid purification or by direct PCR cloning.
characteristics.characteristic: description
characteristics.text: E. coli
characteristics.characteristic: lab-host
characteristics.text: Electric organ
characteristics.characteristic: organ
characteristics.text: Tetronarce californica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7787
characteristics.characteristic: organism
characteristics.text: Electroplaque
characteristics.characteristic: tissue-type
characteristics.text: Torpedo californica electric organ
characteristics.characteristic: title
characteristics.text: pBluescript II KS(+)
characteristics.characteristic: vector