characteristics.text: LIBEST_009654
characteristics.characteristic: External Id
characteristics.text: SAMN00164163
characteristics.characteristic: External Id
characteristics.text: Baylor College of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.400Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A17
characteristics.characteristic: cultivar
characteristics.text: Immature seeds, collected from pods ranging in age from 11 to 19 days after pollination, were harvested from greenhouse-grown plants. Seeds were removed and separated from pod walls and were immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells.
characteristics.characteristic: description
characteristics.text: Immature seeds, 11 to 19 days after pollination
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: immature seeds
characteristics.characteristic: tissue-type
characteristics.text: GESD
characteristics.characteristic: title
characteristics.text: pBluescript SK-
characteristics.characteristic: vector