characteristics.text: LIBGSS_000017
characteristics.characteristic: External Id
characteristics.text: SAMN00150928
characteristics.characteristic: External Id
characteristics.text: University of Texas Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 1997-04-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T13:18:21.470Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Genomic DNA from an Enterococcus faecalis strain TX52 was partially digested with Sau3AI, sized fractionated and ligated into the BamHI site of cosmid vector pBeloBAC11. After in vitro packaging and plating, the cosmid clones were screened with antisera from 4 enterococcal endocarditis patients and a rabbit immunized with surface proteins from an E. faecalis isolate. Cosmid DNA from immunopositive cosmid clones was treated with DNase I, the ends were filled with the Klenow fragment, and then ligated into the EcoRV site in pBluescript SK(-). The ligation mixture was transformed into XL1Blue MRF', and the transformants were screened with antisera. The immunopositive clones were selected for DNA sequencing.
characteristics.characteristic: description
characteristics.text: XL1Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Enterococcus faecalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1351
characteristics.characteristic: organism
characteristics.text: HindIII
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: TX52
characteristics.characteristic: strain
characteristics.text: pBluescript expression library of genomic DNA from TX52
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector