characteristics.text: SAMN00164161
characteristics.characteristic: External Id
characteristics.text: LIBEST_009650
characteristics.characteristic: External Id
characteristics.text: Children's Memorial Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.310Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: CV2 is a subtracted library derived from a normalized eye library (nREE2), constructed according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the CV2 library, plasmid DNA from the normalized rat eye library was electroporated into competent bacteria for production of single-stranded circular DNA. This was then used as a tracer in a subtractive hybridization with a driver comprising: a) a set of about 1,000 arrayed clones from the non-normalized eye library CV0 (PCR amplified inserts from a plasmid DNA template preparation representing plates R-CV0-BRH through R-CV0-BRR). This represented 20% of the final driver population. b) A set of about 4,000 arrayed clones from the normalized eye library CV1 (PCR amplified inserts from a plasmid DNA template preparation representing plates R-CV1-BRS through R-CV1-BSC, R-CV1-BSE through R-CV1-BTC, and R-CV1-BV0 through R-CV1-BVU). This represented 80% of the final driver population.
characteristics.characteristic: description
characteristics.text: ADULT
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: DH10B (Life Technologies)
characteristics.characteristic: lab-host
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Sprague-Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: UI-R-CV2
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector