characteristics.text: LIBEST_022520
characteristics.characteristic: External Id
characteristics.text: SAMN00164754
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:47.067Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ancylostoma caninum L3s recovered from the tissues of mice that had been infected with A. caninum infective L3s (L3s at 10-14 days post infection are considered to be 'tissue arrested' stages) was used for cDNA library construction. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Prema Arasu (Prema_Arasu@ncsu.edu) of North Carolina State University in Raleigh, NC.
characteristics.characteristic: description
characteristics.text: tissue-arrested L3
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Ancylostoma caninum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29170
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Ancylostoma caninum L3 SL1 TOPO v1 Murphy Chiapelli
characteristics.characteristic: title
characteristics.text: plasmid (kanamycin and ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO (Invitrogen)
characteristics.characteristic: vector