characteristics.text: LIBEST_021803
characteristics.characteristic: External Id
characteristics.text: SAMN00153455
characteristics.characteristic: External Id
characteristics.text: MPI for Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:27.800Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: wild population from ambient winter temperature of 3C subjected to temperature stress. lat_lon: 54.82 N 10.81 E. Total RNA was extracted from young leaf tissue and meristematic region of plants collected in wild by SCUBA at ambient temperature of 3C. In ambient seawater in the lab, temperature was increased over 2 days until 17C. Cleaned tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial LD-PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Zostera marina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29655
characteristics.characteristic: organism
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: ZM_E
characteristics.characteristic: title