characteristics.text: LIBEST_009658
characteristics.characteristic: External Id
characteristics.text: SAMN00164167
characteristics.characteristic: External Id
characteristics.text: Harvard University, Howard Hughes Medical Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.493Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Five libraries representing EI0.5/12.5 pancreatic bud, E16.5 pancreas, newborn pancreas, adult pancreas, and adult islets of Langerhans were seperately constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA was made by oligo-dT priming and size-selected by column fractionation. Libraries were amplified once on solid support and plasmid DNA from each library was prepared and mixed in equal amounts. The mixed library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded mixed library plasmid DNA was mixed with 5 micrograms PCR product representing mixed library inserts and hybridized to an Ecot of 6. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.
characteristics.characteristic: description
characteristics.text: Embryonic day 10.5, E12.5, E16.5, newborn, adult, mixed
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Sal I
characteristics.characteristic: re_2
characteristics.text: Both for embryonic & newborn, male for adult and adult islet
characteristics.characteristic: sex
characteristics.text: ICR
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009917
characteristics.characteristic: strain
characteristics.text: Melton Normalized Mixed Mouse Pancreas 1 N1-MMS1
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector