characteristics.text: LIBEST_023390
characteristics.characteristic: External Id
characteristics.text: SAMN00165961
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:13.827Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Asgrow A3237
characteristics.characteristic: cultivar
characteristics.text: Soybean were grown in the UCLA Plant Growth Center with a 16:8 light-dark cycle at 22oC. Total RNA isolated from soybean seeds containing globular-stage embryos was subjected two rounds of poly(A) selection using a Dynabeads oligo(dT) system (Invitrogen). Complementary DNA (cDNA) was generated from 500 ng of poly(A)-selected mRNA using a SMART PCR cDNA Synthesis kit (Clontech) with 20 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs) and then size-fractionated over the Chroma-spin column (Clontech). Fractions containing Sfi I-digested cDNA with size greater than 400 bp were pooled and precipitated. Five micrograms of the size-selected Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for generating Soybean Globular-Stage Seed 454 ESTs.
characteristics.characteristic: description
characteristics.text: Globular-stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0001185
characteristics.characteristic: dev-stage
characteristics.text: Whole seeds
characteristics.characteristic: organ
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: Soybean Seeds Containing Globular-Stage Embryos
characteristics.characteristic: title