characteristics.text: LIBEST_019840
characteristics.characteristic: External Id
characteristics.text: SAMN00152391
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:28.570Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mycelium in compatible wheat (Triticum aestivum) cultivar Thatcher
characteristics.characteristic: cell type
characteristics.text: mRNA obtained by grinding infected leaf tissue & polyA selection; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.
characteristics.characteristic: description
characteristics.text: haustorial, 6 days after inoculation
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B/r
characteristics.characteristic: lab-host
characteristics.text: Puccinia triticina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_208348
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: Apa1
characteristics.characteristic: re_2
characteristics.text: race 1 (design. BBB; Long et al., 1989, Plant Disease 73, 294)
characteristics.characteristic: strain
characteristics.text: PT009
characteristics.ontologyTerms: http://identifiers.org/medgen/C4017280
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBSII(KS)
characteristics.characteristic: vector