characteristics.text: SAMN00153906
characteristics.characteristic: External Id
characteristics.text: LIBEST_011946
characteristics.characteristic: External Id
characteristics.text: Princeton University
characteristics.characteristic: INSDC center name
characteristics.text: 2002-10-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:44.363Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Stem Cells; Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos
characteristics.characteristic: cell type
characteristics.text: Two directionally cloned cDNA libraries were made from fetal liver hematopoietic stem cells enriched to be Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos called Scapos and from AA4.1neg fetal liver cells. Subtractive hybridization was performed by hybridization of the target, Scapos, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, AA4.1neg cDNA library in pSport2 with inserts cloned in the complementary orientation. For detailed protocols and additional information please see our website at http://stemcell.princeton.edu.
characteristics.characteristic: description
characteristics.text: Embryonic day 14-14.5
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Fetal Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: C57Bl/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Hematopoietic
characteristics.characteristic: tissue-type
characteristics.text: Hematopoietic Stem Cell Subtracted Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Sport 1
characteristics.characteristic: vector