characteristics.text: SAMN00154000
characteristics.characteristic: External Id
characteristics.text: LIBEST_013949
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2004-07-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:42.037Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Salivary glands were dissected on day four after initiation of feeding. Total RNA was prepared using acid guanidium thiocyanate-phenol-chloroform extraction. The cDNA library was custom prepared by Invitrogen Corporation. Briefly, first strand cDNA was primed using oligo(dT) containing a NotI site. Size fractionated double stranded cDNA was ligated to EcoRV-NotI cleaved vector and electroporated into E.coli. Library RAB was made from ticks infected with Theileria parva.
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain DH10B-TonA
characteristics.characteristic: lab-host
characteristics.text: Salivary glands
characteristics.characteristic: organ
characteristics.text: Rhipicephalus appendiculatus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_34631
characteristics.characteristic: organism
characteristics.text: Muguga
characteristics.characteristic: strain
characteristics.text: RAB
characteristics.characteristic: title
characteristics.text: pCMVSport6.0.ccdb
characteristics.characteristic: vector