characteristics.text: LIBEST_019664
characteristics.characteristic: External Id
characteristics.text: SAMN00166272
characteristics.characteristic: External Id
characteristics.text: Oregon State University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:24.483Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The subtraction was performed using five day old larvae collected in August of 1998 and the Clontech PCR-Select cDNA subtraction kit according to the manufacturer's instructions. Starting amounts of 1.45 ug mRNA from a 3-bowl mixture of symbiotic larvae (each with an infection rate of 78%) and 1.24 ug mRNA from a 4-bowl mixture of aposymbiotic larvae were used to construct the libraries. cDNAs were inserted into a T/A cloning vector.
characteristics.characteristic: description
characteristics.text: larval day 5 post-fertilization
characteristics.characteristic: dev-stage
characteristics.text: Lobactis scutaria
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_46714
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Fungia scutaria larval sym subtracted library
characteristics.characteristic: title