characteristics.text: SAMN00154110
characteristics.characteristic: External Id
characteristics.text: LIBEST_015793
characteristics.characteristic: External Id
characteristics.text: Agriculture & Agri-Food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2005-05-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:33.747Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DH12075 (double haploid line from Cresor x Westar cross)
characteristics.characteristic: cultivar
characteristics.text: Seeds were collected by Dr. Francois Ouellet when they were still very green (mid to large stage, cotyledons were formed). The seed coats were removed and the remaining tissue was used for cDNA library construction. mRNA was poly-A primed using SuperScript Plasmid System cDNA Synthesis and Cloning kit (Invitrogen) After initial screening, the most abundant redundant clones were screened out using 22 oligos designed to match napins (including albumins), cruciferins, oleosins, trypsin inhibitor 2, cytosolic GAPDH, cyclophilins, HSP70, desaturase, and CAB (LHCP).
characteristics.characteristic: description
characteristics.text: Mid to late embryos (4-6 mg)
characteristics.characteristic: dev-stage
characteristics.text: E. coli ElectroMAX DH5 alpha-e (Invitrogen)
characteristics.characteristic: lab-host
characteristics.text: Embryos without seed coat
characteristics.characteristic: organ
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Brassica embryo library (EL)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1 (modified: GCGGCCGCCc*GACTAGTGAGCTC*cggacgcgtggGTCGAC)
characteristics.characteristic: vector