characteristics.text: SAMN00150449
characteristics.characteristic: External Id
characteristics.text: LIBEST_019834
characteristics.characteristic: External Id
characteristics.text: Forestry and Forest Products Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2006-06-14T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:28.433Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nematodes were cultured on the small pine seedling in germ-free environments. Nematodes were separated by Baermann funnel extraction and washed with 0.1M NaCl and 30% sucrose. mRNA was extracted using Micro-FastTrack (Invitrogen). cDNA library was constructed using the SMART PCR cDNA amplification method (Clontech). SMART-amplified cDNAs were directionally ligated into the pDNR-lib vector. Each plasmid construct was transformed into Escherichia coli DH5a by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).
characteristics.characteristic: description
characteristics.text: Mixed-stage
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5a
characteristics.characteristic: lab-host
characteristics.text: Bursaphelenchus xylophilus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6326
characteristics.characteristic: organism
characteristics.text: Ka4
characteristics.characteristic: strain
characteristics.text: Bursaphelenchus xylophilus cDNA library (Kp)
characteristics.characteristic: title
characteristics.text: pDNR-lib
characteristics.characteristic: vector