characteristics.text: LIBEST_021024
characteristics.characteristic: External Id
characteristics.text: SAMN00152782
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:06.957Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: H898
characteristics.characteristic: cultivar
characteristics.text: The top leaders of 5 mature trees were wounded with a needle at 5 mm intervals on opposite sides of the leader. Bark tissue, with phloem attached, was harvested at 6 hrs, 1, 2, 4, 6 days after treatment initiation. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Normalization was carried out according to published methods (Bonaldo M.F. et al. 1996, Genome Research 6: 791:806).
characteristics.characteristic: description
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Picea sitchensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3332
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of the cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of the cDNA)
characteristics.characteristic: re_2
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Leader bark (phloem and cambium attached) with wounding treatment
characteristics.characteristic: tissue-type
characteristics.text: SS-B-N-22
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector