characteristics.text: LIBEST_019217
characteristics.characteristic: External Id
characteristics.text: SAMN00149880
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2006-02-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:12.870Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.
characteristics.characteristic: description
characteristics.text: DH10B TonA
characteristics.characteristic: lab-host
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRV
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: whole liver, pooled from 20 animals
characteristics.characteristic: tissue-type
characteristics.text: NIH_ZGC_25
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pExpress-1
characteristics.characteristic: vector