characteristics.text: LIBEST_009922
characteristics.characteristic: External Id
characteristics.text: SAMN00164423
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:11.833Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:40:34.927Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ancylostoma caninum SS/L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Prema Arasu (Prema_Arasu@ncsu.edu) of North Carolina State University in Raleigh, NC.
characteristics.characteristic: description
characteristics.text: Serum stimulated L3
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Ancylostoma caninum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29170
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Ancylostoma caninum L3 SS SL1 TOPO v1 Murphy Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid (kanamycin and ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pCRII-TOPO (Invitrogen)
characteristics.characteristic: vector