characteristics.text: LIBEST_008841
characteristics.characteristic: External Id
characteristics.text: SAMN00163361
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:18.650Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This library was constructed by Keliang Tang, Robert Cole, and L. David Sibley at Washington University. cDNAs were synthesized from poly(A)+ RNA by olidod(T) priming, size-selected and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B (GeneHog, Research Genetics, Inc.) for sequencing. WARNING: This library may contain a small percentage contaminants from human fibroblast cells.
characteristics.characteristic: description
characteristics.text: Tachyzoite
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: VEG
characteristics.characteristic: strain
characteristics.text: TgVEG118 Tachyzoite cDNA Library
characteristics.characteristic: title
characteristics.text: pBluescript SK
characteristics.characteristic: vector