characteristics.text: LIBEST_000977
characteristics.characteristic: External Id
characteristics.text: SAMN00155569
characteristics.characteristic: External Id
characteristics.text: The Natural History Museum
characteristics.characteristic: INSDC center name
characteristics.text: 1997-08-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:27.727Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Mohamed Saber (Theodor Bilharz Research Institute, Cairo) from cercariae of an Egyptian strain of Schistosoma mansoni, maintained in Mesocricetus auratus (Golden Hamster) and Biomphalaria alexandrina . Total mRNA was isolated using the Guanadine thiocyanate / phenol-chloroform method and poly (A)+ RNA purified by affinity chromatography on an oligo d(T) column . The cDNA library was unidirectionally constructed, by ligation of the restricted, double stranded cDNA into the EcoRI and XhoI restriction sites of Lambda ZapII.
characteristics.characteristic: description
characteristics.text: cercaria
characteristics.characteristic: dev-stage
characteristics.text: Mesocricetus auratus (definitive), Biomphalaria alexandrina (intermediate)
characteristics.characteristic: lab-host
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Egyptian
characteristics.characteristic: strain
characteristics.text: TBRI-Saber-cercaria-A
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Zap II
characteristics.characteristic: vector