characteristics.text: SAMN00155467
characteristics.characteristic: External Id
characteristics.text: LIBEST_000869
characteristics.characteristic: External Id
characteristics.text: Moredun Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 1997-05-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:23.913Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA library was constructed by Diane Redmond (Moredun Research Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH, UK). mRNA was prepared from adult Trichostrongylus vitrinus isolated from the small intestine of a lamb, 21 days after the lamb was infected with T. vitrinus infective L3. The mRNA was converted to first-strand cDNA using reverse transcriptase and an oligo(dT) primer. Following treatment with RNase H, the second cDNA strand was generated by using DNA polymerase I. The library had a total titre of 1.25 x 10E6 phage.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Trichostrongylus vitrinus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_40352
characteristics.characteristic: organism
characteristics.text: male & female
characteristics.characteristic: sex
characteristics.text: Moredun
characteristics.characteristic: strain
characteristics.text: whole worm
characteristics.characteristic: tissue-type
characteristics.text: Trichostrongylus vitrinus adult cDNA lambda gt11 library
characteristics.characteristic: title