characteristics.text: SAMN00155461
characteristics.characteristic: External Id
characteristics.text: LIBEST_000862
characteristics.characteristic: External Id
characteristics.text: Washington University School of MedicineP
characteristics.characteristic: INSDC center name
characteristics.text: 1997-05-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:23.760Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb.
characteristics.characteristic: description
characteristics.text: 2-cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001285
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: MluI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: C57BL/6J x DBA/2J F1
characteristics.characteristic: strain
characteristics.text: embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: Knowles Solter mouse 2 cell
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pBluescribe (modified)
characteristics.characteristic: vector