characteristics.text: SAMN00152910
characteristics.characteristic: External Id
characteristics.text: LIBEST_021171
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:11.370Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: tissue and cell isolation according to Weisser et al. (2002); RNA isolation via guanidinthiocyanat method of Chomcynski and Sacchi (1987): after polyA+ purification first strand synthesis with a Not I primer-adaptor; second strand synthesis (nick translation Gubler and Hoffmann, 1983) and ligation of Sal I adaptor; Size separation of cDNA fragments and target cloning in vector pSPORT1
characteristics.characteristic: description
characteristics.text: fetal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000323
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Bos taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Sal I
characteristics.characteristic: re_2
characteristics.text: growth plate
characteristics.characteristic: tissue-type
characteristics.text: Bt_FGP library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector