characteristics.text: SAMN00154080
characteristics.characteristic: External Id
characteristics.text: LIBEST_015608
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2004-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:28.400Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Fal-1028
characteristics.characteristic: cultivar
characteristics.text: Bark was wounded using razor blades along the entire length of the tree at 5 mm intervals on opposite sides of the trunk. The same trees were also sprayed with a 0.01% (v/v) methyl jasmonate solution resuspended in 0.1% (v/v) tween 20 (~50mLs per tree). Bark tissue with phloem attached was harvested 3 hours, 6 hours, 12 hours, 24 hours, 2 days, 4 days and 8 days after initiating the treatment. Untreated control bark was also harvested at time 0 hours. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.
characteristics.characteristic: description
characteristics.text: E. coli DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Bark (with phloem and cambium attached) from one year old clonal trees grown under greenhouse conditions in standard potting soil mixture
characteristics.characteristic: organ
characteristics.text: Picea engelmannii x Picea glauca
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_373101
characteristics.characteristic: organism
characteristics.text: EcoRI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: XhoI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: IS-B-N-A-10
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector