characteristics.text: LIBEST_000577
characteristics.characteristic: External Id
characteristics.text: SAMN00155180
characteristics.characteristic: External Id
characteristics.text: San Francisco General Hospital-University of California, San Francisco
characteristics.characteristic: INSDC center name
characteristics.text: 1996-12-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:14.793Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was isolated from purified Cryptosporidium parvum sporozoites using TRIZOL reagent (GIBCO-BRL). Directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I linkers, and digestion with Xho I, all using the Stratagene ZAP-cDNA synthesis kit. The cDNA was cloned into the EcoR I and Xho I sites of Lambda Uni-ZAP XR vector; the primary library was >97% recombinant and contained 1.3 X 10(6) independent clones with an ca. average insert size of 1.3 kb. Based on open reading frame (orf) analysis of the first 64 sequence tags we estimate that up to one-third of the library is composed of genomic DNA clones since approximately 15% of the orfs were incorrectly oriented on the antisense strand.
characteristics.characteristic: description
characteristics.text: sporozoite
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1 Blue MRF' Kan
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: IOWA
characteristics.characteristic: strain
characteristics.text: uniZAPCpIOWAsporoLib1
characteristics.characteristic: title
characteristics.text: UniZAP XR
characteristics.characteristic: vector