characteristics.text: SAMN00164370
characteristics.characteristic: External Id
characteristics.text: LIBEST_009867
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-10-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:48.877Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized from poly mRNA using an oligo-dT primer containing an XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size- selected on sephacryl S500. The cDNAs were ligated to EcoRI/ XhoI prepared lambda ZapII (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Insert sizes range from 0.3-3.0 kb. The library may contain a small percentage of host or bacterial contaminents. NOTE: Many clones lack the 5' EcoRI site but contain the upstream polylinker sites.
characteristics.characteristic: description
characteristics.text: Tachyzoite
characteristics.characteristic: dev-stage
characteristics.text: SOLR cells
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: VEG (Type III)
characteristics.characteristic: strain
characteristics.text: TgVEG-Tachyzoite cDNA
characteristics.characteristic: title
characteristics.text: pBluescript SK-
characteristics.characteristic: vector