characteristics.text: LIBGSS_000008
characteristics.characteristic: External Id
characteristics.text: SAMN00150915
characteristics.characteristic: External Id
characteristics.text: Harvard Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 1997-01-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T13:18:19.050Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: No library was made. Genomic DNA flanking mariner transposon insertions was amplified by inverse PCR from individual Leishmania major lines. PCR primers were complementary to transposon sequences. Two sets were used, one for the 5' half and the other for the 3' half of the transposon. PCR products were cloned in the pGEM-T vector and sequenced using the PCR primers.
characteristics.characteristic: description
characteristics.text: Leishmania major
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5664
characteristics.characteristic: organism
characteristics.text: MHOM/IR/83/LT252
characteristics.characteristic: strain
characteristics.text: Random insertions of transposon mariner in Leishmania major
characteristics.characteristic: title