characteristics.text: LIBEST_000471
characteristics.characteristic: External Id
characteristics.text: SAMN00155080
characteristics.characteristic: External Id
characteristics.text: International Livestock Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 1996-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:20:21.340Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNAs were generated from poly (A+) enriched mRNA prepared from different developmental stages of T.b.brucei by reverse transcription followed by PCR amplification using mini-exon and oligo(dT) primers. The cDNA generated were utilized in RADES-PCR coupled with differential hybridisations to identify differentially expressed mRNA transcripts. The products which showed to be differentially expressed were cloned pGEM -T vector. These differentially expressed mRNA transcripts were (are being) sequenced to generate differentially expressed sequence tags.
characteristics.characteristic: description
characteristics.text: Trypanosoma brucei brucei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5702
characteristics.characteristic: organism
characteristics.text: Clone A4
characteristics.characteristic: strain
characteristics.text: Bloodstream form of serodeme ILTat1.1
characteristics.characteristic: title