characteristics.text: SAMN00164786
characteristics.characteristic: External Id
characteristics.text: LIBEST_022551
characteristics.characteristic: External Id
characteristics.text: Shanghai Institute of Veterinary Research, Chinese Academy of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2008-06-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-01-08T23:05:06.793Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from 44d adult worms of Schistosoma japonicum by Trizol reagent and mRNA was isolated from the total RNA. Then, ds cDNA was synthesized by reverse transcription using random primers. After that, directional EcoRI/HindIII linkers were ligated into the ends of ds cDNA and then digested, so two blunt ends EcoRI and HindIII were formed. The digested ds cDNA fragments longer than 300bp in length were fractionated, and then ligated into T7 Select 10-3b vector. After packaging in vitro, the T7 Select 10-3b vector was transformed into BLT5403 to construct the T7 phage display cDNA library.
characteristics.characteristic: description
characteristics.text: Adult worm 44d
characteristics.characteristic: dev-stage
characteristics.text: Schistosoma japonicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6182
characteristics.characteristic: organism
characteristics.text: Chinese mainland strain
characteristics.characteristic: strain
characteristics.text: Schistosoma japonicum 44d adult worm phage display cDNA library
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: T7 Select 10-3b vector
characteristics.characteristic: vector