characteristics.text: LIBEST_021259
characteristics.characteristic: External Id
characteristics.text: SAMN00154420
characteristics.characteristic: External Id
characteristics.text: Warsaw Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:13.610Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA was isolated using Chomczynski method with Trizol reagent. 100 micrograms of RNA was used to amplification using primers designed to Zea mays viviparus 1 sequence from GenBank. The RT-PCR product was cloned into the TOPO vector and transformed the recombinant vector into competent E. coli.
characteristics.characteristic: description
characteristics.text: immature embryos
characteristics.characteristic: organ
characteristics.text: Secale cereale
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4550
characteristics.characteristic: organism
characteristics.text: Inbred line L318
characteristics.characteristic: strain
characteristics.text: Secale cereale viviparus 1 TOPO TA Cloning
characteristics.characteristic: title
characteristics.text: pUC plasmid
characteristics.characteristic: v_type
characteristics.text: PCRII-TOPO
characteristics.characteristic: vector