characteristics.text: SAMN00155496
characteristics.characteristic: External Id
characteristics.text: LIBEST_000898
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1997-06-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:25.507Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: RNA was prepared from a pool of 6 anonymous Wilms' tumor RNAs. RNA was prepared by acid-phenol, followed by one round of oligo dT selection. cDNA library preparation was with the BRL/Life Tech. Superscript Plasmid system. An oligo-dT NotI primer for first strand synthesis generated gcggccgccc(t)n at the 3' end of the clones. A 5' SalI adaptor was used with sequence 5'-gtcgacccacgcgtccg-3'. Resulting cDNAs were size selected (average size 2 kb), NotI digested, and ligated into NotI/SalI-cut pSPORT1. Library was constructed by Dr. Manfred Gessler.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: pooled (6)
characteristics.characteristic: sex
characteristics.text: Gessler Wilms tumor
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector