characteristics.text: LIBEST_023816
characteristics.characteristic: External Id
characteristics.text: SAMN00166774
characteristics.characteristic: External Id
characteristics.text: Centro de Investigacion en Alimentacion y Desarrollo A.C.
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:28.280Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The mRNA was isolated from Alternaria alternata strains adapted to lethal levels of 2-propenyl-isothiocyanate according to the protocol published by Islas-Flores et al., 2006 (Molecular Biotechnology 34(1): 45-50). The cDNAs were prepared using the SMART PCR cDNA synthesis kit and subtracted by SSH procedure (Clontech, Palo Alto CA). The diferentially expressed cDNAs were cloned into p-GEM-T Easy vector and cells of E. coli JM109 were transformed with them (Promega, Madison, WI). Rsa I was used to digest the recombinant clones and verify the presence and sizes of the inserted fragments. Sequenciation of fragments was carried out using using the M13 forward primer in the Genome Analysis and Technologic Core Facility of the University of Arizona at Tucson, AZ.
characteristics.characteristic: description
characteristics.text: Alternaria alternata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5599
characteristics.characteristic: organism
characteristics.text: Rsa I
characteristics.characteristic: re_1
characteristics.text: Rsa I
characteristics.characteristic: re_2
characteristics.text: Mycelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001436
characteristics.characteristic: tissue-type
characteristics.text: Alternaria alternata treated with 2-pITC library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: p-GEMT Easy vector
characteristics.characteristic: vector