characteristics.text: SAMN00152282
characteristics.characteristic: External Id
characteristics.text: LIBEST_016610
characteristics.characteristic: External Id
characteristics.text: Cornell University
characteristics.characteristic: INSDC center name
characteristics.text: 2004-10-22T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:58.243Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ailsa Craig
characteristics.characteristic: cultivar
characteristics.text: A yeast signal sequence trap (YSST) cDNA library was constructed by using poly(A) mRNA isolated from an equal weight mixture of tomato fruits at several different developmental and ripening stages. Poly(A) mRNA was purified from total RNA using an Oligotex mRNA Midi Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. The first-strand cDNAs were synthesized from mRNA using N6-NotI primer (5'-GAGAGAGAGAGAGAGAACCGCGCGGCCGCCNNNNNN-3') by using a cDNA Synthesis Kit (Stratagene). After second-strand synthesis the cDNAs were prepared for unidirectional cloning by ligation with EcoRI adapters according to the manufacturer's instructions, followed by NotI digestion. The cDNA fragments (0.3 to 1.0 kb) were recovered with a Gel Extraction Kit (Qiagen) from agarose gel, and ligated to an equipartite mixture of the three vectors, pYSST0, pYSST1, and pYSST2, digested with EcoRI and NotI. Ligations were transformed into electrocompetent TOP10 E. coli (Invitrogen) by electroporation.
characteristics.characteristic: description
characteristics.text: Developmental and ripening stages
characteristics.characteristic: dev-stage
characteristics.text: fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organ
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: fruit pericarp
characteristics.characteristic: tissue-type
characteristics.text: Tomato fruit related cDNA library-yeast signal sequence trap 1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pYSST
characteristics.characteristic: vector