characteristics.text: SAMN00156953
characteristics.characteristic: External Id
characteristics.text: LIBEST_002392
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-09-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:05.967Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This C elegans library was made from fem-3(q23) worms (produce only sperm at 25 C). cDNA was generated via oligo (dT) priming and directionally cloned into pBluescript II SK+ vector with a modified polylinker. This library was subtracted with a second fem-1(hc17) (produce only oocytes at 25 C) library to enrich this library for sperm specific genes. First strand cDNA synthesis was primed with a NotI-15T oligo (sequence 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'). After 2nd strand synthesis, XhoI-EcoRI adaptors (Stratagene #901120) were ligated, and cDNA was digested with NotI and ligated into XhoI-NotI digested vector.
characteristics.characteristic: description
characteristics.text: DH5alpha cells
characteristics.characteristic: lab-host
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: C elegans fem3 Q23 S1
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK+
characteristics.characteristic: vector