characteristics.text: SAMN00163187
characteristics.characteristic: External Id
characteristics.text: LIBEST_008661
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:13.213Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Merozoites were obtained from ceacal scrapings of chickens infected with E. tenella. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. The cDNAs were ligated to EcoRI/XhoI prepared lambda ZAPII(Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Clones were selected by negative hybridization against a pool of over-represented ESTs (N>=10, from 1506 previous reads), and transformed to DH10B (GENEHOGS, STRATAGENE). The library may contain a small percentage of host or bacterial contaminents.
characteristics.characteristic: description
characteristics.text: Merozoite
characteristics.characteristic: dev-stage
characteristics.text: DH10B (GENEHOG, E.coli HS996)
characteristics.characteristic: lab-host
characteristics.text: Eimeria tenella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5802
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: LS18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006640
characteristics.characteristic: strain
characteristics.text: Eimeria tenella M5-6 cDNA Neg Selected
characteristics.characteristic: title
characteristics.text: phagemid (Amp. resistant)
characteristics.characteristic: v_type
characteristics.text: Bluescript SK-
characteristics.characteristic: vector