characteristics.text: LIBEST_000347
characteristics.characteristic: External Id
characteristics.text: SAMN00154959
characteristics.characteristic: External Id
characteristics.text: Yale University Medical School
characteristics.characteristic: INSDC center name
characteristics.text: 1990-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:09.063Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified from the ocular ciliary body of an adult female donor. A unidirectional cDNA library was constructed using lambda-Uni-ZAP XR vector (Stratagene, Ca) as described by Escribano et al. (J. Biochem. 1995). Using lambda DNA from the cDNA library as template and the set of primers indicated a 412-bp product was obtained, directly subcloned in the TA Cloning vector (pCR II) (Invitrogen Corp., San Diego, Ca), and further sequenced from the 5' and 3' ends. Forward Primer: CTACCAGACGGGCTCAGTGTTG Reverse Primer: GGGCCAGCTTGTCAGTCTCCT
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Human ocular ciliary body cDNA library 2
characteristics.characteristic: title
characteristics.text: TA
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005309
characteristics.characteristic: vector