characteristics.text: SAMN00150339
characteristics.characteristic: External Id
characteristics.text: LIBEST_019724
characteristics.characteristic: External Id
characteristics.text: National Institute of Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-06-04T21:03:09.068Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Protonemata cultivated on the BCDATG medium were blended by the POLYTRON, and then cultivated on the BCDATG medium at 25C for a week under continuous light. Colonies of protonemata were inoculated on a peat pot and cultivated for 1-1.5 months at 25C under continuous light. Upper part of gametophores with approximately 15 leaves was dissected for RNA extraction. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of 1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo, Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3'end primer 5'-CGACCTGCAGCTCGAGCACA-3'. synonym: Physcomitrella patens subsp. patens
characteristics.characteristic: description
characteristics.text: gametophores
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030026
characteristics.characteristic: dev-stage
characteristics.text: Physcomitrium patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: DraIII
characteristics.characteristic: re_1
characteristics.text: DraIII
characteristics.characteristic: re_2
characteristics.text: Gransden2004
characteristics.characteristic: strain
characteristics.text: Upper half part of gametophores
characteristics.characteristic: tissue-type
characteristics.text: ppls full-length cDNA library
characteristics.characteristic: title
characteristics.text: pME18S-FL3
characteristics.characteristic: vector