characteristics.text: SAMN00156436
characteristics.characteristic: External Id
characteristics.text: LIBEST_001871
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1999-06-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:53.143Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E. tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. cDNAs were digested with EcoRI/XhoI and cloned into lambda Zap II (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and SOLR cells (Stratagene ). Insert sizes range from 1.2-2.9 kb.
characteristics.characteristic: description
characteristics.text: Sporozoite
characteristics.characteristic: dev-stage
characteristics.text: SOLR E. coli
characteristics.characteristic: lab-host
characteristics.text: Eimeria tenella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5802
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: LS18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006640
characteristics.characteristic: strain
characteristics.text: Eimeria S5-2 Sporozoite stage
characteristics.characteristic: title
characteristics.text: phagemid (Amp. resistant)
characteristics.characteristic: v_type
characteristics.text: Bluescript SK-
characteristics.characteristic: vector