characteristics.text: SAMN00155957
characteristics.characteristic: External Id
characteristics.text: LIBEST_001374
characteristics.characteristic: External Id
characteristics.text: Smith College Department of Biological Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 1998-07-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:38.927Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Lymphatic filarial nematode parasite of humans. cDNA from L3, microfilaria, adult male and adult female libraries was isolated and amplified by PCR using T3 and T7 primers. Microfilaria, adult male and adult female cDNA was subtracted from L3 cDNA using the PCR-select cDNA subtraction kit (Clontech). The library had 4.0 x 105 independent recombinants and the average insert size is ~500bp. The library was constructed by Lori Saunders. The library is available from Dr. Steven A. Williams, email: genome@smith.edu
characteristics.characteristic: description
characteristics.text: L3 cDNA subtracted with microfilaria, adult male and adult female cDNA libraries
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Brugia malayi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6279
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: Brugia malayi L3 subtracted cDNA library (SAW97LS-BmL3SD)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP II
characteristics.characteristic: vector