characteristics.text: LIBEST_018780
characteristics.characteristic: External Id
characteristics.text: SAMN00154268
characteristics.characteristic: External Id
characteristics.text: University of California
characteristics.characteristic: INSDC center name
characteristics.text: 2005-12-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:02.773Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total accessory gland RNA was isolated using the Ambion mirVana miRNA kit and was then RNAsed. cDNA was synthesized (Invitrogen GeneRacer kit; the SSIII module and oligo(dT) primer were used for the RT step), amplified (5 cycles) using the Roche Expand High Fidelity PCR System, purified (Qiagen QIAquick PCR Purification kit), incubated in Promega Taq polymerase and ligated into PCR4 TOPO vector
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: Accessory Gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0010147
characteristics.characteristic: organ
characteristics.text: Drosophila yakuba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7245
characteristics.characteristic: organism
characteristics.text: Male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Tai18E2
characteristics.characteristic: strain
characteristics.text: Male Accessory Gland Library Drosophila yakuba
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: PCR4TOPO
characteristics.characteristic: vector