characteristics.text: SAMN00150778
characteristics.characteristic: External Id
characteristics.text: LIBEST_020209
characteristics.characteristic: External Id
characteristics.text: Key Laboratory of Forest Plant Ecology (KLP), Ministry of Education
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:41.383Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The greenhouse grown corktree seedlings from DAB (days after burgeoning) 60 to 65 were rapidly removed from the pumice by washing and drying on absorbent papers before placing intact in a climatic chamber(day cycle). Leaves were separated then frozen in liquid N2 after 2 h of dehydration. Total RNA was extracted by Trizol Reagent (Invitrogen). mRNA was isolateded and purified from total RNA (Qiagen). Library construction followed the user manual of Clotech PCR-Select cDNA subtraction Kit.
characteristics.characteristic: description
characteristics.text: DAB (days after burgeoning) 60 to 65
characteristics.characteristic: dev-stage
characteristics.text: TOP10 cells
characteristics.characteristic: lab-host
characteristics.text: Phellodendron amurense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_68554
characteristics.characteristic: organism
characteristics.text: NcoI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Amur corktree subtraction cDNA library under water stress
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector