characteristics.text: LIBEST_020659
characteristics.characteristic: External Id
characteristics.text: SAMN00152453
characteristics.characteristic: External Id
characteristics.text: Embrapa Recursos Geneticos e Biotecnologia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-02-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:56.110Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Arachis stenosperma seeds were germinated in sterile soil. Roots inoculated with Bradirhyzobium sp. were collected from three-month old plants. Collected materials were immediately frozen in liquid nitrogen for RNA extraction. Total RNA was isolated from plant materials using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture's instructions. Poly (A)+ RNA was extracted from 1 mg of total RNA using the Oligotex Spin Column (Qiagen Inc., Valencia, CA, USA) according to the manufacture's protocol. Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in lambdaTriplEx2 (Clontech, Palo Alto, CA, USA). The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained using the mass in-vivo excision protocol according to the manufacture`s instructions (Clontech, USA). The white clones grown on screening LB medium (Amp/IPTG/X-Gal) were recovered by random colony selection. All cDNA inserts were sequenced from the 5'-end using specifically designed primer: PT2F2 5'-GCGCCATTGTGTTGGTACCC-3'.
characteristics.characteristic: description
characteristics.text: Mature plant
characteristics.characteristic: dev-stage
characteristics.text: Accession V10309 (in Embrapa Genetic Resources and Biotechnology Germplasm bank)
characteristics.characteristic: isolate
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Arachis stenosperma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_217475
characteristics.characteristic: organism
characteristics.text: Root
characteristics.characteristic: tissue-type
characteristics.text: Roots inoculated with Bradirhyzobium sp. (RI)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: pTriplEx2 (Clontech, Palo Alto, CA, USA).
characteristics.characteristic: vector