characteristics.text: SAMN00154704
characteristics.characteristic: External Id
characteristics.text: LIBEST_000087
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:06.063Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:19:45.547Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: mRNA was purified by guanidium isothiocyanate from log-phase cells of Pyrobaculum aerophilum using media described by Huber et. al., (Arch. Microbiol. 144:324-333). cDNA was prepared using the lambda ZAP kit (Stratagene). RNA was primed with an oligo dT primer and/or a random primer containing an XhoI site in the first direction. After generating the lambda ZAP library, plasmids were excised for double-strand sequencing.
characteristics.characteristic: description
characteristics.text: SURE (Stratagene)
characteristics.characteristic: lab-host
characteristics.text: Pyrobaculum aerophilum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13773
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Pyrobaculum aerophilum ESTMT library
characteristics.characteristic: title
characteristics.text: pBLUESCRIPT II (Stratagene)
characteristics.characteristic: vector