characteristics.text: LIBEST_004023
characteristics.characteristic: External Id
characteristics.text: SAMN00158576
characteristics.characteristic: External Id
characteristics.text: Duke University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-04-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:48.780Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Strain CC-125 was grown to mid-log phase in TAP medium, washed twice in TAP-S (sulfur-deficient TAP medium) and incubated in TAP-S. RNA was isolated after 1, 2 and 4 hours. An equal amount of RNA from each time point was pooled and polyA mRNA purified. cDNA was synthesized and directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage.
characteristics.characteristic: description
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: CC-125 wild type mt+ 137C
characteristics.characteristic: strain
characteristics.text: C. reinhardtii CC-125 -S, Lambda Zap II
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK-
characteristics.characteristic: vector