characteristics.text: SAMN00003005
characteristics.characteristic: External Id
characteristics.text: University of Washington Genome Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2009-05-29T11:30:08.210Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:50:14.293Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS002999
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS002999
characteristics.characteristic: SRA accession
characteristics.text: For the lin-35(n745) L1s, we grow the strain at 20 deg until majority of animals are gravid. 2) We bleach the worms to collect embryos, then place the embryos in S-basal buffer and incubate at 20 deg overnight to hatch into L1s. 3) We then put the hatched ~7K L1s on each of 5-6 seeded NGM plates at 20 deg, grow ~3 days until gravid again 4) We then bleach the worms for embryos, put the embryos in S-basal and incubate at 20 deg overnight to hatch into starved L1s. 5) We then place all L1s onto one large seeded plate for 4 hours at 25 deg. 6) We then wash off all worms from food and let them digest their ingested food for one hour in S basal 7) We spin down, monitor pellet size, and then add four volumes of Trizol, perform three rounds of freeze/thaw, and submit for RNA isolation. Run 30KCMAAXX = 150-250 bp size selection, 1% agarose gel, GAPipeline-1.0 Run 30k3FAAXX = 250-350 bp size selection, 1% agarose gel, GAPipeline-1.0 Run 311T6AAXX, 311U1AAXX = 250-350 bp size selection, 1.5% agarose gel, GAPipeline-1.3 Quality values = solexa/illumina (not converted to phred)
characteristics.characteristic: description
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Generic sample from Caenorhabditis elegans
characteristics.characteristic: title