characteristics.text: LIBEST_005583
characteristics.characteristic: External Id
characteristics.text: SAMN00160121
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-Food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:36.990Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This cDNA library was constructed from polyA+ enriched mRNA from zoospores grown in liquid medium. Zoospores were released into water and collected by centrifugation at 2,000g; zoospore-bearing sporangia were induced to develop on 5 to 7 d old mycelium colonies grown on V8 agar by repeated flooding with water. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.
characteristics.characteristic: description
characteristics.text: zoospores
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Phytophthora sojae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_67593
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: race 2, strain P6497
characteristics.characteristic: strain
characteristics.text: PsojaeZO
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBK-CMV
characteristics.characteristic: vector