characteristics.text: SAMN00016996
characteristics.characteristic: External Id
characteristics.text: UNIL
characteristics.characteristic: INSDC center name
characteristics.text: 2011-02-01T04:06:24.580Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:58:17.053Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS084969
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS084969
characteristics.characteristic: SRA accession
characteristics.text: 24 adult males were collected from 12 nests (two males per colony) throughout northeastern Argentina. All individuals were combined into a single pool and total RNA was extracted using Trizol (Invitrogen). Total mRNA was isolated using the OligoDirect mini mRNA extraction kit following the manufacturer’s suggested protocol (Qiagen). Double stranded cDNA libraries were constructed using the SMART cDNA synthesis kit (Clontech) following the manufacturer’s suggested protocol. Approximately 500 nanograms of mRNA was used as starting material. Each of the four cDNA libraries were then normalized using the TRIMMER kit (Evrogen).
characteristics.characteristic: description
characteristics.text: Solenopsis invicta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13686
characteristics.characteristic: organism
characteristics.text: 24 adult males, 2 males per colony, Argentina
characteristics.characteristic: title