characteristics.text: LIBEST_022135
characteristics.characteristic: External Id
characteristics.text: SAMN00153716
characteristics.characteristic: External Id
characteristics.text: State University of Campinas
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:37.027Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: somatic
characteristics.characteristic: cell type
characteristics.text: Total RNA was extracted from the dikaryotic saprotrophic mycelium of M. perniciosa grown for 14 days on minimal media containing glucose as the only carbon source. Library was prepared from total RNA using the BD Creator TM SMART TM cDNA Library Construction kit (BD Biosciences-ClontechTM, USA, Palo Alto, CA), following the manufacturer's protocol, and cloned into the pDNR-LIB plasmid vector provided in the kit.
characteristics.characteristic: description
characteristics.text: saprotrophic
characteristics.characteristic: dev-stage
characteristics.text: Theobroma cacao L.
characteristics.characteristic: lab-host
characteristics.text: Moniliophthora perniciosa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_153609
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: CP02
characteristics.characteristic: strain
characteristics.text: dikaryotic mycelium
characteristics.characteristic: tissue-type
characteristics.text: E-Sap-Glu
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB (BD Biosciences-ClontechTM, USA, Palo Alto, CA)
characteristics.characteristic: vector