characteristics.text: SAMN00006258
characteristics.characteristic: External Id
characteristics.text: Oregon St Univ - Center for Genome Research & Biocomputing
characteristics.characteristic: INSDC center name
characteristics.text: 2010-01-08T12:55:46.400Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:52:10.193Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS008432
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS008432
characteristics.characteristic: SRA accession
characteristics.text: Pinus monticola MONT06E1
characteristics.characteristic: barcode AACT
characteristics.text: Pinus monticola MONT07E2
characteristics.characteristic: barcode ATGT
characteristics.text: Pinus monticola MONT08E1
characteristics.characteristic: barcode CACT
characteristics.text: Pinus monticola MONT14E1
characteristics.characteristic: barcode GGGT
characteristics.text: Pinus monticola MONT12E1
characteristics.characteristic: barcode GTAT
characteristics.text: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
characteristics.characteristic: description
characteristics.text: Pinus monticola
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3345
characteristics.characteristic: organism
characteristics.text: Generic sample from Pinus monticola
characteristics.characteristic: title