characteristics.text: SAMN00152713
characteristics.characteristic: External Id
characteristics.text: LIBEST_020957
characteristics.characteristic: External Id
characteristics.text: UC Davis, Department of Pomology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.140Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Paradox clone 'PX1'
characteristics.characteristic: cultivar
characteristics.text: Five micropropagated plants of the walnut paradox clone PX1 were grown in the greenhouse. On April 5, 2006 each plant was inoculated with ~6400 individual Pratylenchus vulnus nematodes. On May 19, 2006 the leaves of these plants were harvested and frozen in liquid nitrogen and stored at -80C. RNA was isolated from the leaves obtained from the individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit) from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen) to obtain individual colonies that were sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).
characteristics.characteristic: description
characteristics.text: Small greenhouse plants
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10-Beta
characteristics.characteristic: lab-host
characteristics.text: Root
characteristics.characteristic: organ
characteristics.text: Juglans hindsii x Juglans regia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_432290
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: WLN-02: walnut leaf from plants infected with Pratylenchus vulnus
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector