characteristics.text: LIBEST_018240
characteristics.characteristic: External Id
characteristics.text: SAMN00165015
characteristics.characteristic: External Id
characteristics.text: University of Wisconsin
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-03T23:44:12.140Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:41:34.723Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Nipponbare
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007587
characteristics.characteristic: cultivar
characteristics.text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and universal CRR primer CRR_ppt1F (5' CAA GAA GGG GAG GAT GAT G 3'). To make PCR more specific and sensitive, the PCR products were re-amplified using AUAP_3'RACE primer in combination with primer specific for CRR1 subfamily (CRR1_1F; 5' TGA TGA GGA CAT CCC TTC C 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.
characteristics.characteristic: description
characteristics.text: four weeks old plant
characteristics.characteristic: dev-stage
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Oryza sativa Japonica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: organism
characteristics.text: LIB_ID:20
characteristics.characteristic: title