characteristics.text: SAMN00161855
characteristics.characteristic: External Id
characteristics.text: LIBEST_007325
characteristics.characteristic: External Id
characteristics.text: Heska Corporation
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:32.580Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was made using the PCR-Select( cDNA Subtraction kit (CLONTECH Laboratories, Inc.) with 2 (g hindgut and Malpighian tubule mRNA used as to make the 'tester' cDNA and 2 (g carcass (all flea tissues minus the hindgut and Malpighian tubules) to make the 'driver' cDNA. Suppression PCR and subtractive hybridization techniques are then used to enrich for cDNAs in the tester pool that are not also present in the driver pool.
characteristics.characteristic: description
characteristics.text: unfed adult and 24 hour cat blood-fed adults (1:1 ratio)
characteristics.characteristic: dev-stage
characteristics.text: Ctenocephalides felis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7515
characteristics.characteristic: organism
characteristics.text: female and male (4:1 ratio)
characteristics.characteristic: sex
characteristics.text: hindgut and Malpighian tubule
characteristics.characteristic: tissue-type
characteristics.text: hindgut and Malpighian tubule subtracted cDNA library
characteristics.characteristic: title