characteristics.text: LIBEST_009610
characteristics.characteristic: External Id
characteristics.text: SAMN00164121
characteristics.characteristic: External Id
characteristics.text: Fox Chase Cancer Center
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.270Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNAs were synthesized by reverse transcription of mRNAs purified from the liver and a hepatocellular carcinoma (HCC) of a woodchuck (marmota monax) chronically infected with woodchuck hepatitis virus. cDNAs were digested with RsaI and ligated to one of two different adaptors. HCC enriched cDNA was then generated using the Clontech PCR Select cDNA subtraction kit. Amplicons with non-identical adaptors at each end were selectively amplified by PCR, using primers specific to the adaptors, and the resulting amplicons were ligated into pGEM T easy (Promega).
characteristics.characteristic: description
characteristics.text: Liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Marmota monax
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9995
characteristics.characteristic: organism
characteristics.text: Hepatocellular carcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000182
characteristics.characteristic: tissue-type
characteristics.text: hepatocellular carcinoma expression library
characteristics.characteristic: title