characteristics.text: SAMN00152406
characteristics.characteristic: External Id
characteristics.text: LIBEST_020616
characteristics.characteristic: External Id
characteristics.text: The Samuel Roberts Noble Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2006-11-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:54.953Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Guar (Cyamopsis tetragonoloba) plants, cultivar HES 1401, were grown in 2 gallon pots containing 75% Metro Mix 350 soil and 25% sand at a temperature of 26oC/22oC (day/night). Plants were fertilized at time of watering using a commercial Peters Professional 20-10-20 (N-P-K) fertilizer mix. Seeds were harvested 15, 20, 25, 30, 35, and 40 days after flowering (DAF). cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. An 'early' seed library was generated from seeds 15, 20, and 25 DAF. The cDNA was directionally ligated into the Uni-Zap XR vector and packaged using Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF'. Excised plasmids were plated using SOLR cells.
characteristics.characteristic: description
characteristics.text: early developmental stage (15-25 days after flowering, DAF)
characteristics.characteristic: dev-stage
characteristics.text: Cyamopsis tetragonoloba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3832
characteristics.characteristic: organism
characteristics.text: EcoRI (GAATTC)
characteristics.characteristic: re_1
characteristics.text: XhoI (CTCGAG)
characteristics.characteristic: re_2
characteristics.text: seed
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009010
characteristics.characteristic: tissue-type
characteristics.text: Cyamopsis tetragonoloba (L.) Taub seeds at early developmental stage
characteristics.characteristic: title
characteristics.text: pBluescript sk
characteristics.characteristic: vector