characteristics.text: LIBEST_015371
characteristics.characteristic: External Id
characteristics.text: SAMN00152271
characteristics.characteristic: External Id
characteristics.text: Institute of BioAgricultural Sciences, Academia Sinica
characteristics.characteristic: INSDC center name
characteristics.text: 2004-08-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:22.537Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: CL5915
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006368
characteristics.characteristic: cultivar
characteristics.text: Tomato plants were grown hydroponically in modified Hoagland's solution. Three-week-old plants were subjected for excess copper treatment in which 10 ugM CuSO4 is applied to the nutrient solution. Roots were harvested after four days of continuous treatment. Total and poly A+ RNA was isolated from treated and control roots, respectively. Subtraction cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under excess copper condition.
characteristics.characteristic: description
characteristics.text: 26-day-old
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH 5alpha
characteristics.characteristic: lab-host
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: roots
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: tissue-type
characteristics.text: Subtraction cDNA library of root under excess copper
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T Easy Vector
characteristics.characteristic: vector