characteristics.text: SAMN00166648
characteristics.characteristic: External Id
characteristics.text: LIBEST_023689
characteristics.characteristic: External Id
characteristics.text: CSIR Biosciences
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:22.577Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ICML12=P7
characteristics.characteristic: cultivar
characteristics.text: RNA was extracted from leaves using a Plant RNeasy Kit (Qiagen). mRNA was purified from total RNA with an Oligotex mRNA Mini Kit (Qiagen). Double stranded cDNA was synthesised using a cDNA Synthesis System (Roche). Differentially expressed cDNAs were isolated from treated leaves using a PCR-Select cDNA Subtraction Kit (BD Biosciences, Clontech). cDNA enriched for differentially expressed transcripts was cloned into pGEMT-Easy, and transformed into E. coli DH10B cells.
characteristics.characteristic: description
characteristics.text: Ten day old seedlings
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Cenchrus americanus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4543
characteristics.characteristic: organism
characteristics.text: leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Ten day old pearl millet seedlings treated with defence elicitors
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEMT-Easy
characteristics.characteristic: vector