characteristics.text: LIBEST_004913
characteristics.characteristic: External Id
characteristics.text: SAMN00159461
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-06-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:12.857Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was prepared from zebrafish(C32) fin, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is non-normalized. Library was constructed by Ning Wu. NOTE: This clone is available royalty-free through LLNL; contact the IMAGE Consortium (info.llnl.gov)for further information
characteristics.characteristic: description
characteristics.text: GeneHogs (HS996, a phage-resistant isolate of DH10B)
characteristics.characteristic: lab-host
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Fin
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_4000164
characteristics.characteristic: tissue-type
characteristics.text: Zebrafish Research Genetics C32 fin
characteristics.characteristic: title
characteristics.text: Phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector