characteristics.text: SAMN00154280
characteristics.characteristic: External Id
characteristics.text: LIBEST_018943
characteristics.characteristic: External Id
characteristics.text: Temasek Lifesciences Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:06.610Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA was synthesized from 80-day-old carp testis total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.
characteristics.characteristic: description
characteristics.text: 80 days post fertilization
characteristics.characteristic: dev-stage
characteristics.text: developing testis
characteristics.characteristic: organ
characteristics.text: Cyprinus carpio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7962
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: all-male (XY) offspring of XX females and androgenetic YY males
characteristics.characteristic: strain
characteristics.text: 80-day-old common carp testis (full-length)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBS-SK-Sfi
characteristics.characteristic: vector