characteristics.text: LIBEST_022516
characteristics.characteristic: External Id
characteristics.text: SAMN00164750
characteristics.characteristic: External Id
characteristics.text: Root Biology Center, South China Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:40:51.273Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: BX10
characteristics.characteristic: cultivar
characteristics.text: Soybean genotype BX10 was hydroponically grown in the greenhouse with an average temperature of 28/19C (day/night) and relative humidity ranging from 50%-80%. One week later, the seedlings were transplanted into one-half-strength modified Hoagland solution containing 250 uM KH2PO4. The seedlings were grown for ten days till the first trifolia were fully developed. Furthermore, plants treated with 0.2 uM Pi (1000 uM Pi as control) for 0.5, 1, 6, and 12 h were used as the materials for construction of short-term Pi starvation-induced cDNA libraries, while plants treated with 0.2 uM Pi (1000 uM Pi as control) for 3, 6, 9, and 12 d were used as the materials for construction of long-term Pi starvation-induced cDNA libraries, respectively. The cDNAs generated from plants at low P level (0.2 uM Pi) served as testers, while cDNAs generated from plants at high P level (1000 uM Pi) were used as drivers. Totally three SSH libraries were constructed, including a root short-term library (RS), a root long-term library (RL), and a shoot long-term library (SL). Correspondingly, RNA from shoots and roots subjected to Pi starvation at 0.5, 1, 6 and 12 h was separately extracted and pooled for construction of root short-term library and a shoot short-term library, while RNA from shoots and roots subjected to Pi starvation at 3, 6, 9 and 12 d was separately extracted and pooled for construction of root long-term library and a shoot long-term library.
characteristics.characteristic: description
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: tissue-type
characteristics.text: Glycine max cv. BX10 root long-term (RL) SSH library
characteristics.characteristic: title
characteristics.text: pGem-T easy
characteristics.characteristic: vector