characteristics.text: LIBEST_000976
characteristics.characteristic: External Id
characteristics.text: SAMN00155568
characteristics.characteristic: External Id
characteristics.text: The Natural History Museum
characteristics.characteristic: INSDC center name
characteristics.text: 1997-08-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:27.703Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was prepared commercially by Stratagene in Uni-ZAP XR using mRNA from in vitro cultured, 7 day schistosomula derived from mechanically-transformed cercaria (cultured by Richard Harrop, University of York). mRNA was reverse transcribed using oligo dT as primer and the cDNA was directionally cloned between the EcoRI and XhoI sites of the vector. Primary titre = 1.8x10e6, amplified titre = 7.7x10e9. The library contains 9.5% non recombinant clones and significant contamination with E. coli sequences (approx 20% of clones).
characteristics.characteristic: description
characteristics.text: lung stage (7 day, in vitro cultured schistosolula)
characteristics.characteristic: dev-stage
characteristics.text: Biomphalaria glabrata
characteristics.characteristic: lab-host
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Schistosoma mansoni
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6183
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Puerto Rica
characteristics.characteristic: strain
characteristics.text: York-Harrop-lung-A
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Uni-ZAP XR
characteristics.characteristic: vector