characteristics.text: SAMN00155061
characteristics.characteristic: External Id
characteristics.text: LIBEST_000451
characteristics.characteristic: External Id
characteristics.text: Chinese National Human Genome Center at Shanghai
characteristics.characteristic: INSDC center name
characteristics.text: 1996-06-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:11.660Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Several hundred adult Schistosoma japonicum (Anhui, P.R. China, strain), of mixed sex, were perfused from the mesenteries of experimentally infected mice and rabbits at the Queensland Institute of Medical Research, Brisbane, Australia (QIMR), and stored for several months in liquid nitrogen. Subsequently, mRNA was isolated at the QIMR from lysates of these worms by oligo dT chromatography, using a kit from Pharmacia. The mRNA was then shipped to Clontech, Palo Alto, CA, USA, who constructed a cDNA library. First strand synthesis was primed with an oligo-dT-XhoI-primer and synthesized using M-MLV reverse transcriptase. Second strand synthesis was accomplished with RNAse H and T4 DNA polymerase. The double stranded cDNA was ligated to EcoRI linkers, digested with EcoRI and XhoI, and ligated into the phagemid vector lambda ZAP II XR. After construction of this directional library by Clontech, it was returned to the QIMR. During analysis of the library at the QIMR, we have found that a small percentage, 2% to 3%, of the clones contain inserts that appear to be highly homologous to sequences from salmonoid fishes, as determined by homology comparisons using BLAST and by Southern hybridization analysis to genomic DNA from salmon (Sigma Chemical Co., St. Louis, MO) under stringent washing conditions. The remainder of the clones appear to contain S. japonicum sequences.
characteristics.characteristic: description
characteristics.text: Adult worms
characteristics.characteristic: dev-stage
characteristics.text: Mouse and rabbit
characteristics.characteristic: lab-host
characteristics.text: Schistosoma japonicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6182
characteristics.characteristic: organism
characteristics.text: EcoR I
characteristics.characteristic: re_1
characteristics.text: Xhol I
characteristics.characteristic: re_2
characteristics.text: Male and female
characteristics.characteristic: sex
characteristics.text: Chinese (Anhui) strain
characteristics.characteristic: strain
characteristics.text: Whole body
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001489
characteristics.characteristic: tissue-type
characteristics.text: Adult SjC 7/94
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP-II XR.
characteristics.characteristic: vector