characteristics.text: SAMN00164807
characteristics.characteristic: External Id
characteristics.text: LIBEST_022572
characteristics.characteristic: External Id
characteristics.text: The University of Georgia
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:49.103Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: OMF164
characteristics.characteristic: cultivar
characteristics.text: cDNA libraries were constructed from Limnanthes alba OMF164, an open-pollinated population. Total RNAs from immature embryos were isolated with Trizol (Invitrogen). The cDNAs above 500 bp were used for cDNA library construction. cDNA libraries were constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into Clontech pDNR-LIB vector after Sfi I digestion. The clones were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Limnanthes alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_42439
characteristics.characteristic: organism
characteristics.text: SfiIA (GGCCATTACGGCC)
characteristics.characteristic: re_1
characteristics.text: SfiIB (GGCCGAGGCGGCC)
characteristics.characteristic: re_2
characteristics.text: Immature embryo
characteristics.characteristic: tissue-type
characteristics.text: Limnanthes alba EST Library MF1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB (Clontech)
characteristics.characteristic: vector