characteristics.text: LIBEST_002484
characteristics.characteristic: External Id
characteristics.text: SAMN00157045
characteristics.characteristic: External Id
characteristics.text: University of Wisconsin
characteristics.characteristic: INSDC center name
characteristics.text: 2000-01-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:08.623Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: First strand cDNA was primed with a NotI-polyT primer [5'-AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT-3']. Double-stranded cDNA was ligated with EcoRI adapters (Pharmacia), digested with NotI, and ligated into the EcoRI/NotI sites of the pGEM11Zf vector. The library was NOT normalized.
characteristics.characteristic: description
characteristics.text: JM109
characteristics.characteristic: lab-host
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: mouse liver, vehicle control
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM11Zf (Promega)
characteristics.characteristic: vector