characteristics.text: LIBEST_017590
characteristics.characteristic: External Id
characteristics.text: SAMN00154225
characteristics.characteristic: External Id
characteristics.text: University of British Columbia
characteristics.characteristic: INSDC center name
characteristics.text: 2005-07-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:29.140Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: FB3-425
characteristics.characteristic: cultivar
characteristics.text: Adult white pine weevils (Pissodes strobi) were caged with mesh bags on sapling trees. Bark tissue with phloem and cambium attached was harvested 2 hours, 6 hours and 48 hours after continuous feeding by the insects. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with XhoI (5' end) and BamHI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.
characteristics.characteristic: description
characteristics.text: E. coli DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Bark (with phloem and cambium attached) from two year old clonal trees grown under greenhouse conditions in standard potting soil mixture
characteristics.characteristic: organ
characteristics.text: Picea sitchensis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3332
characteristics.characteristic: organism
characteristics.text: XhoI (5' end of cDNA)
characteristics.characteristic: re_1
characteristics.text: BamHI (3' end of cDNA)
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: SS-IB-A-FL-15
characteristics.characteristic: title
characteristics.text: pBluescript II SK (+) XR
characteristics.characteristic: vector