characteristics.text: LIBEST_023693
characteristics.characteristic: External Id
characteristics.text: SAMN00166652
characteristics.characteristic: External Id
characteristics.text: INRA - INSA de Lyon
characteristics.characteristic: INSDC center name
characteristics.text: 2009-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:22.677Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Suppressive subtractive hybridization (SSH) technology was applied to cDNA from E. coli infected larvae and cDNA from naive larvae. The cDNA substracted library was made using a PCR-selected cDNA subtraction kit (Clontech). Sized cDNAs were directly ligated into pCR2.1 TOPO. T7 and M13rev primers were used for EST sequencing. WARNING: the library may contains a small percentage of contamination (rDNA, bacterial DNA...); Differential gene expression between naive and E. coli infected aphids was not confirmed.
characteristics.characteristic: description
characteristics.text: Aposymbiotic fourth instar larvae (Rifampicin-treated aphids)
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli TOP10
characteristics.characteristic: lab-host
characteristics.text: Whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Acyrthosiphon pisum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7029
characteristics.characteristic: organism
characteristics.text: LL01 (Lusignan, France)
characteristics.characteristic: strain
characteristics.text: Aphid Immune Substracted library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1 TOPO
characteristics.characteristic: vector