characteristics.text: SAMN00153923
characteristics.characteristic: External Id
characteristics.text: LIBEST_012358
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles
characteristics.characteristic: INSDC center name
characteristics.text: 2002-12-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:56.100Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hammond's Dwarf Scarlet
characteristics.characteristic: cultivar
characteristics.text: Embryo-proper regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from embryo-proper mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The embryo-proper cDNA fragments were directionally ligated into the SfiI restriction site of the lambda TriplEx2 vector (Clontech), and the recombinant cDNAs were transformed into E. coli XL1-Blue cells (Clontech). Embryo-proper cDNA plasmids used for directional sequencing were obtained by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 cells (Clontech).
characteristics.characteristic: description
characteristics.text: 6-days post-pollination
characteristics.characteristic: dev-stage
characteristics.text: Embryo-Proper Region of Globular-Stage Embryos
characteristics.characteristic: organ
characteristics.text: Phaseolus coccineus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3886
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: Scarlet Runner Bean Embryo-Proper Region
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: TriplEx2
characteristics.characteristic: vector