characteristics.text: LIBEST_020102
characteristics.characteristic: External Id
characteristics.text: SAMN00150680
characteristics.characteristic: External Id
characteristics.text: University of Oklahoma
characteristics.characteristic: INSDC center name
characteristics.text: 2006-08-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:37.767Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sperm cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001005
characteristics.characteristic: cell type
characteristics.text: cDNA was prepared from isolated Plumbago zeylanica sperm cells and amplified by PCR with the help of SMART PCR cDNA Synthesis kit (Clontech). Suppression subtractive hybridizations between Sua and Svn sperm cells were then performed using the PCR-Select cDNA Subtraction kit (Clontech).The purified subtractive Sua and Svn cDNAs were cloned into pCR2.1-TOPO vector (Invitrogen) and transformed into Escherichia coli XL10-Gold cells (Stratagene) to obtain subtracted Sua and Svn cDNA libraries, respectively.
characteristics.characteristic: description
characteristics.text: mature pollen of Plumbago zeylanica
characteristics.characteristic: dev-stage
characteristics.text: E.coli XL10-Gold
characteristics.characteristic: lab-host
characteristics.text: Plumbago zeylanica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_76149
characteristics.characteristic: organism
characteristics.text: Subtracted cDNA library of Plumbago zeylanica Svn sperm cell
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1-TOPO
characteristics.characteristic: vector