characteristics.text: SAMN00000747
characteristics.characteristic: External Id
characteristics.text: Western Washington University
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-14T13:20:52.820Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:43:01.283Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS001171
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS001171
characteristics.characteristic: SRA accession
characteristics.text: We sampled a variety of different life stages of Rhagoletis pomonella ranging from larvae to adults. We collected larvae (stages L2 and L3) from field-infested apples in Urbana, IL, during the summer of 2007. Larvae were dissected from infested apples and washed with water before being shock-frozen on dry ice. We obtained pupae from infested hawthorn fruit in South Bend, IN, during the fall of 2006. Infested fruit was transported to the laboratory where larvae emerged and pupated. One set of pupae was transferred to 4C after a prediapause period of two weeks and kept under diapause conditions for 4 months. A second set of pupae was kept at 25C until eclosion. Samples were taken from each set of individuals in regular intervals and shock-frozen at -80C. After removal from diapause additional samples were taken from the first set representing post-diapause developmental stages. Hawthorn race adults were obtained when pupae held at 25C eclosed. Apple race adults were caught on fruit in an apple orchard in Urbana, IL, in the summer of 2008. Live adults were shock-frozen on dry ice and the heads were separated from the adult bodies
characteristics.characteristic: description
characteristics.text: Rhagoletis pomonella
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_28610
characteristics.characteristic: organism
characteristics.text: Generic sample from Rhagoletis pomonella
characteristics.characteristic: title