characteristics.text: LIBEST_023610
characteristics.characteristic: External Id
characteristics.text: SAMN00166503
characteristics.characteristic: External Id
characteristics.text: ICRISAT
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:20.247Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: JL 24: rd29A:DREB1A transgenic
characteristics.characteristic: cultivar
characteristics.text: Partial sequence obtained by DDRT-PCR from salt induced leaves. Peanut plants were grown in MS agar tubes in the tissue culture lab and after two weeks of germination plants were transferred to test tubes containing MS liquid medium and plants were grown for one week in liquid medium. Three weeks old plants were subjected to salt stress treatments by transferring in to a fresh tube containing MS liquid plus 200 mM NaCl. Leaf tissue samples were collected from non-transgenic JL 24 and transgenic JL 24:rd29A:DREB1A stressed plants and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated with TRIzol Reagent (Invitrogen life technologies, CA 92008, USA) from the leaf samples. Differential display of cDNA was performed using RNAimage (GenHunter Corporation, TN 37211, USA) kit following the manufacturer's protocol. Differentially expressed transcripts were isolated from the gel. Re-amplified PCR fragments were cloned in to pGem-T Easy Vector (Promega Madison, WI 53711-5399 USA) and sequenced using M13 primers. Sequences are trimmed to remove vector and cloning oligo sequences contaminants.
characteristics.characteristic: description
characteristics.text: 21 day-old seedlings
characteristics.characteristic: dev-stage
characteristics.text: E.coli DH10B
characteristics.characteristic: lab-host
characteristics.text: Arachis hypogaea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3818
characteristics.characteristic: organism
characteristics.text: salt-stressed leaves
characteristics.characteristic: tissue-type
characteristics.text: peanut (JL 24:rd29A:DREB1A transgenic) salt stressed differentially expressed cDNA
characteristics.characteristic: title
characteristics.text: pGEM-T Easy (Promega)
characteristics.characteristic: vector