characteristics.text: LIBEST_020813
characteristics.characteristic: External Id
characteristics.text: SAMN00152588
characteristics.characteristic: External Id
characteristics.text: US Army Engineer Research and Development Center-Environmental Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:00.633Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Two cDNA libraries were constructed using the SSH technique. The first SSH library was made using pooled mRNA (10 microgram) extracted from control unexposed worms against worms exposed to Cd (2.6 mmol/kg), TNT (100 mg/kg), 2,6-DNT (54 mg/kg), RDX (50 mg/kg), or HMX (10 mg/kg), whereas mRNA (10 microgram) from worms exposed to Cu (293 mg/kg), Pb (8778 mg/kg), Zn (357 mg/kg), 2,4-DNT (100 mg/kg), and TNB (100 mg/kg) was run against mRNA from another set of control worm for the second library. Exposure (4-, 14-, or 28-d) was conducted in an Organization for Economic Cooperation and Development (OECD) artificial soil consisting of 70% sand, 20% kaolin clay, and 10% 2-mm sieved peat moss with an adjusted pH between 6.5 and 7.0. Exposed and unexposed earthworms were fixed in RNAlater (Ambion) and stored at -80oC. Total RNA was extracted using RNeasy kit (Qiagen), and poly(A) mRNA was purified using NucleoTrap mRNA purification kit (BD Biosciences). The Clontech PCR-Select cDNA subtraction kit (BD Biosciences) was then used to enrich for differentially expressed genes.
characteristics.characteristic: description
characteristics.text: adults
characteristics.characteristic: dev-stage
characteristics.text: Eisenia fetida
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6396
characteristics.characteristic: organism
characteristics.text: whole worm
characteristics.characteristic: tissue-type
characteristics.text: Earthworm SSH cDNA library
characteristics.characteristic: title