characteristics.text: LIBEST_021122
characteristics.characteristic: External Id
characteristics.text: SAMN00152874
characteristics.characteristic: External Id
characteristics.text: Stanford Human Genome Center
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:10.067Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Vector: Express 1; The cDNA library was constructed from O.lucimarinus strain (CCE9901) isolated by B. Palenik from 32,9000 N 117,2550 W (Scripps Institution of Oceanography Pier, La Jolla, California, USA) and was grown as reported previously (Worden, A.Z, Nolan, J.K., and Palenik, B (2004) Limnology and Oceanography 49 , 168-179). Total and poly A+ RNA was isolated and a cDNA library was constructed in the Express 1 plasmid vector by Open Biosystems. First strand cDNA synthesis was primed with an 54 bp linker primer containing an oligodT sequence preceeded by a synthetic NotI site (first strand primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'). Following second strand synthesis, cDNAs were made blunt at the end corresponding to the original 5 prime end of mRNA, and cloned directionally into the NotI and EcoRV sites of Express 1. Note that the EcoRV site is typically destroyed in the blunt end cloning, leaving a junction of the form 'xxxATC'(where is ATC is the second half of the EcoRV site, and xxx is derived from the cDNA sequence). A map of the Express 1 vector is available at: http://www.openbiosystems.com/cdna_library_construction_faq.php#8 The primary library was transformed and amplified in DH10B (T1 phage resistant) bacteria.
characteristics.characteristic: description
characteristics.text: DH10B (T1 phage resistant)
characteristics.characteristic: lab-host
characteristics.text: Ostreococcus lucimarinus CCE9901
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_436017
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRV
characteristics.characteristic: re_2
characteristics.text: CCE9901
characteristics.characteristic: strain
characteristics.text: Whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: SHGC-CTZ
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pExpress
characteristics.characteristic: vector