characteristics.text: LIBEST_009686
characteristics.characteristic: External Id
characteristics.text: SAMN00164193
characteristics.characteristic: External Id
characteristics.text: Tufts University School of Veterinary Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-08-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:44.280Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Obtained by 5'RACE following the protocol of Gibco BRL. cDNA was synthesized using a gene-specific (IL1-ra) primer (GSP1: 5'-CTGTTTGAGCGGATGAAGGT-3') from a genomic sequence. dCTP was used to add a homopolymeric tail in the 3'end and the tailed cDNA was amplified by PCR using an anchor IL-1ra gene-specific primer (GSP2: 5'-CAUCAUCAUCAUCTGTTTGAGCGGATGAAGGTGAA-3') as the reverse and a Universal Anchor Primer (Gibco BRL) as the forward primer. cDNA was cloned through UDG cloning method.
characteristics.characteristic: description
characteristics.text: Foal
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH5
characteristics.characteristic: lab-host
characteristics.text: Equus caballus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9796
characteristics.characteristic: organism
characteristics.text: Blood cells (buffy coat) from a septic female
characteristics.characteristic: tissue-type
characteristics.text: 5'RACE cDNA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAMP1 (Gibco BRL)
characteristics.characteristic: vector