characteristics.text: SAMN00166509
characteristics.characteristic: External Id
characteristics.text: LIBEST_023616
characteristics.characteristic: External Id
characteristics.text: Embrapa Genetic Resources & Biotechnology
characteristics.characteristic: INSDC center name
characteristics.text: 2008-08-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:20.390Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Cachaco
characteristics.characteristic: cultivar
characteristics.text: The dehydration-tolerant banana variety Cachaco (ABB cooking banana). Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN - Protocol: Purification of total RNA from plant cells and tissues and filamentous fungi). Then, the mRNA was obtained using the Micro-FastTrack 2.0 Kit (Invitrogen - Protocol: mRNA isolation from total RNA + Basic mRNA isolation method). The mRNA was then used to produce the cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech Laboratories - Protocol: SMART cDNA synthesis by LD PCR + Ligation of ds cDNA to pDNR-LIB). The PrimeScript Reverse transcriptase (TAKARA BIO INC) and the Advantage 2 PCR Kit (Clontech Laboratories) were also used in the preparation of this cDNA library.
characteristics.characteristic: description
characteristics.text: Cauliflower-like shoot meristems submitted to 2-week acclimation on 0.4M sucrose
characteristics.characteristic: dev-stage
characteristics.text: ITC.0643
characteristics.characteristic: isolate
characteristics.text: Cauliflower-like shoot meristems
characteristics.characteristic: organ
characteristics.text: Musa ABB Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_214693
characteristics.characteristic: organism
characteristics.text: Sfi IA
characteristics.characteristic: re_1
characteristics.text: Sfi IB
characteristics.characteristic: re_2
characteristics.text: Hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: Embrapa_Musa_Cachaco_ABB_Stressed
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector