characteristics.text: LIBEST_009532
characteristics.characteristic: External Id
characteristics.text: SAMN00164045
characteristics.characteristic: External Id
characteristics.text: University of Colorado at Boulder
characteristics.characteristic: INSDC center name
characteristics.text: 2001-06-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:40.060Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SL1-PCR-TOPO based library from L3 worms isolated from hamsters. Ancylostoma ceylanicum cDNA was amplified by PCR, using an SL1-EcoRI primer directed to the 5' end and oligo(dT)-XhoI directed to 3' end. PCR products were gel fractionated, and non-directionally cloned into pCR-TOPO-XL (Invitrogen), following the XL-Topo TA cloning protocol. The cDNA insert can be excised with EcoRI. The library was constructed by Dr. Vadim Kapulkin and Adia Magalska at Department of Infectious Diseasaes, Immunology and Parasitology of Veterinary Faculty at the Warsaw Agricultural University. The Ancylostoma ceylanicum strain used in this work was provided by Dr. Jerzy Behnke at the University of Nottingham.
characteristics.characteristic: description
characteristics.text: activated, 3rd stage larvae (L3)
characteristics.characteristic: dev-stage
characteristics.text: Top10F' or DH10B
characteristics.characteristic: lab-host
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Ancylostoma ceylanicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_53326
characteristics.characteristic: organism
characteristics.text: female and male
characteristics.characteristic: sex
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: tissue-type
characteristics.text: Vadim Kapulkin Ancylostoma ceylanicum SL1-PCR sub-library
characteristics.characteristic: title
characteristics.text: pCR-TOPO-XL
characteristics.characteristic: vector