characteristics.text: LIBEST_007316
characteristics.characteristic: External Id
characteristics.text: SAMN00161846
characteristics.characteristic: External Id
characteristics.text: National Eye Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:32.340Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Post-mortem iris tissue was pooled from 10 individuals ranging in age from 4-80 years and RNA was extracted. From this pooled sample an aliquot of 60ug of total RNA yielded 2.17ug of mRNA. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies, essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).
characteristics.characteristic: description
characteristics.text: Adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: EMDH10B
characteristics.characteristic: lab-host
characteristics.text: Eye
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000970
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Iris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001769
characteristics.characteristic: tissue-type
characteristics.text: Human Iris cDNA (Un-normalized, unamplified): BX
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCMVSPORT6
characteristics.characteristic: vector