characteristics.text: LIBEST_005595
characteristics.characteristic: External Id
characteristics.text: SAMN00160133
characteristics.characteristic: External Id
characteristics.text: New Mexico State University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:37.417Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Spores were germinated in the presence of 1% CO2. At 10 days, tissue was ground in liquid nitrogen with sand and mRNA isolated with a modified hot phenol/SDS method. The SMART-PCR method (Clontech) was used for cDNA synthesis from 1 ug of total RNA followed by directional cloning in SfiI digested Triplex2 vector. Plasmid subclones in pTriplEx were recovered by cre-lox excision in E. coli strain BM25.8 and sequenced from the 5' end with the 5'Triplex sequencing primer (Tpx) and the 3' end with the T7 primer (T7). (sfi1=__bp in Comment line of EST file is estimated insert size)
characteristics.characteristic: description
characteristics.text: 10 days after germination
characteristics.characteristic: dev-stage
characteristics.text: E. coli BM 25.8
characteristics.characteristic: lab-host
characteristics.text: Rhizophagus intraradices
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4876
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: germinating spore tissue
characteristics.characteristic: tissue-type
characteristics.text: GS Lambda-Triplex, 10 day germinating spore library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Triplex2
characteristics.characteristic: vector