characteristics.text: SAMN00158671
characteristics.characteristic: External Id
characteristics.text: LIBEST_004120
characteristics.characteristic: External Id
characteristics.text: Fred Hutchinson Cancer Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2000-05-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:50.923Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Avian leukosis virus-induced lymphoma
characteristics.characteristic: cell type
characteristics.text: DT-40
characteristics.characteristic: cell-line
characteristics.text: A cDNA library was prepared using both oligo dT and random primers and cloned into the Eco R1 site of lamda ZAP (strategene). This base library was subtracted with mRNA from normal 2-week bursa, and with an excess of avian leukosis virus, c-myc, and immunoglobulin sequences, by the method of Sive and St. John (A simple subtractive hybridization technique employing photoactivatible biotin and phenol extraction. Nucleic Acids Research 16, 10937, 1988)to produce DT40subNB.
characteristics.characteristic: description
characteristics.text: bursal stem cell
characteristics.characteristic: dev-stage
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: bursa of Fabricius
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003903
characteristics.characteristic: tissue-type
characteristics.text: DT40subNB
characteristics.characteristic: title