characteristics.text: SAMN00004465
characteristics.characteristic: External Id
characteristics.text: Wayne State University School of Medicine and PRB/NICHD/NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2010-09-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:50:29.277Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS004934
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS004934
characteristics.characteristic: SRA accession
characteristics.text: Loxodonta africana placental tissue was kindly provided by Dr. Kurt Benirschke of the San Diego Zoo and preserved in RNAlater (Ambion, Foster City, CA). Placental villous trees were separated from the extraembryonic membranes, and total RNA was isolated from the pooled placental compartments using TRIzol Reagent (Invitrogen, Carlsbad, CA). The Qiagen RNeasy kit was used in conjunction with the Qiagen Rnase-Free Dnase Set for cleanup (Qiagen, Valencia, CA, USA) according to the manufacturer 2019s recommendations. The concentration and purity of the total RNA isolated from the placental villous trees was tested by using the Nanodrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE). Total RNA (15*g) was submitted to Agencourt Bioscience Corporation (Beverly, MA, USA) per the company’s instructions.
characteristics.characteristic: description
characteristics.text: Loxodonta africana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9785
characteristics.characteristic: organism
characteristics.text: Generic sample from Loxodonta africana
characteristics.characteristic: title