characteristics.text: SAMN00017034
characteristics.characteristic: External Id
characteristics.text: DOE JGI
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-16T19:27:32.753Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:57:55.780Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS085007
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS085007
characteristics.characteristic: SRA accession
characteristics.text: nitrosative stress; DPTA-NONOate
characteristics.characteristic: assay
characteristics.text: YPD
characteristics.characteristic: culture media
characteristics.text: For the nitrosative stress experiments, a saturated overnight culture of strain SC5314 was diluted into 600 mls of YPD 80mM HEPES pH7.5 to an OD600 of ~0.1 and allowed to grow at 30*C until an OD600 of ~1.0. At this point the culture was split into 2 - 250 ml cultures in separate 1L flasks. To one of the cultures, a 750mM stock solution of DPTA-NONOate (Cayman chemicals; dissolved in 10mM NaOH) was added to a final concentration of 1mM. An equal volume of 10mM NaOH was added to the other 250 ml culture as an untreated control. After 15 minutes of treatment at 30*C the cells were harvested by vacuum filtration and immediately stored at -80*C for later RNA isolation.
characteristics.characteristic: description
characteristics.text: Candida albicans SC5314
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_237561
characteristics.characteristic: organism
characteristics.text: SC5314
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006481
characteristics.characteristic: strain
characteristics.text: C. albicans SC5314 grown under non-nitrosative environment (control)
characteristics.characteristic: title