characteristics.text: SAMN00155056
characteristics.characteristic: External Id
characteristics.text: LIBEST_000446
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 1996-06-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:11.550Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Toxoplasma parasites were grown in human foreskin fibroblasts cultures in vitro. The library was constructed by A. Hehl and I. Manger, Stanford University. cDNAs were synthesized from polyA mRNAs by oligo d(T) priming and directionally cloned into the EcoRI and XhoI sites of the Lambda Zap vector using the ZAP-cDNA synthesis Kit (Statagene). Warning: the library contains a small percentage of human cDNAs derived from the human host cells.
characteristics.characteristic: description
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Toxoplasma gondii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5811
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: ME49, clone PDS
characteristics.characteristic: strain
characteristics.text: TgME49 Tachyzoite cDNA
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda ZAP
characteristics.characteristic: vector