characteristics.text: LIBEST_003756
characteristics.characteristic: External Id
characteristics.text: SAMN00158312
characteristics.characteristic: External Id
characteristics.text: Bernhard Nocht Institute for Tropical Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2000-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:41.433Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Filarial nematode parasite of humans. Three adult female worms of Onchocerca volvulus were isolated from one consenting patient from western Uganda. The patient was treated 26 hours before nodulectomy with a single dose of 150 mg/kg ivermectin. Adult female worms were quick frozen. Their mRNA was converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 6 x 10E4 independent recombinants and the average insert size is ~920bp. The library was constructed by Peter Fischer with worms provided by Dr. Dietrich W. Buttner. The library is available from Dr. Peter Fischer (Germany), Pfischer@bni.uni-hamburg.de
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF'
characteristics.characteristic: lab-host
characteristics.text: Onchocerca volvulus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6282
characteristics.characteristic: organism
characteristics.text: Eco RI
characteristics.characteristic: re_1
characteristics.text: Xho I
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: O. volvulus adult female cDNA 26 h following ivermectin (PF99PF-OvAF26)
characteristics.characteristic: title
characteristics.text: Phage
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR
characteristics.characteristic: vector