characteristics.text: SAMN00166714
characteristics.characteristic: External Id
characteristics.text: LIBEST_023755
characteristics.characteristic: External Id
characteristics.text: National Institute of Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-06-04T21:03:09.471Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Gametophores were cultivated on sterile peat pellets (Jiffy-7; Jiffy Products International AS, Kristansand, Norway) for 1 to 1.5 months at 25C under continuous light. The plants were moved to 15C under 8-h light/16-h dark conditions to induce gametangia. The gametophores were cultivated at 15C under the short day conditions for 31 to 53 days to collect sporophyte tissue during meiosis. Archegonial tissue was removed and only sporophytic tissue was collected. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library constructed by Yutaka Suzuki and Sumio Sugano (University of Tokyo, Institute of Medical Science). synonym: Physcomitrella patens subsp. patens
characteristics.characteristic: description
characteristics.text: Physcomitrium patens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3218
characteristics.characteristic: organism
characteristics.text: DraIII
characteristics.characteristic: re_1
characteristics.text: DraIII
characteristics.characteristic: re_2
characteristics.text: Gransden
characteristics.characteristic: strain
characteristics.text: green sporophytes
characteristics.characteristic: tissue-type
characteristics.text: ppgs full-length cDNA library; green sporophytes
characteristics.characteristic: title
characteristics.text: pME18S-FL3
characteristics.characteristic: vector