characteristics.text: LIBEST_021293
characteristics.characteristic: External Id
characteristics.text: SAMN00153024
characteristics.characteristic: External Id
characteristics.text: Queensland Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2007-08-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:14.533Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA reverse transcribed from serum-stimulated and non-stimulated control A. caninum L3 was subjected to suppression subtractive hybridization (SSH) in order to produce libraries enriched for differentially expressed mRNAs. The forward subtracted library contains cDNAs enriched for mRNAs up-regulated upon serum stimulation while the reverse subtracted library is comprised of cDNAs enriched for mRNAs that are down-regulated upon serum stimluation. Following subtractive hybridization, cDNAs were subjected to two rounds of suppresive PCR using primers that selectively amplify DNA hybrids representing differenitally expressed mRNAs. End product PCRs were cloned into the pGEM-T T/A cloning vector and transformed into TOP10 chemically competent cells. 958 and 171 sequences were obtained from the forward and reverse subtracted libraries, respectively.
characteristics.characteristic: description
characteristics.text: Third stage larvae (cDNAs enriched from serum stimulated L3)
characteristics.characteristic: dev-stage
characteristics.text: Ancylostoma caninum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_29170
characteristics.characteristic: organism
characteristics.text: Multiple isolates from Brisbane, Australia
characteristics.characteristic: strain
characteristics.text: Forward Subtracted Ancylostoma caninum L3 library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEMT-T
characteristics.characteristic: vector