characteristics.text: LIBEST_008894
characteristics.characteristic: External Id
characteristics.text: SAMN00163411
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:20.043Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. David Bird of North Carolina State University.
characteristics.characteristic: description
characteristics.text: enriched for eggs
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Meloidogyne javanica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6303
characteristics.characteristic: organism
characteristics.text: Meloidogyne javanica egg pAMP1 v6 Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pAMP1 (Gibco)
characteristics.characteristic: vector