characteristics.text: SAMN00164119
characteristics.characteristic: External Id
characteristics.text: LIBEST_009608
characteristics.characteristic: External Id
characteristics.text: Yale University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-07-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:42.220Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Murine hematopoietic progenitor
characteristics.characteristic: cell type
characteristics.text: EML and EPRO cells
characteristics.characteristic: cell-line
characteristics.text: EEE library was differentiated from cDNA subtracted by EML(0h) cultured in SCF only, EML (6h) treated with SCF (100ng/ml), Interleukin-3 (30ng/ml) and all-trans retinoic acid (ATRA) and EPRO cultured in SCF/IL-3/ATRA for 72h with 20ng/ml murine GM-CSF cells. EML (0h), EML (6h), and EPRO cDNA were directionally cloned into EcoR1-XhoI restriction site of the (ZAP-express (Stratagene). The ligated cDNA fragments were transformed into DH10B host cells.
characteristics.characteristic: description
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: EEE (0hEML, 6hEML and EPRO)
characteristics.characteristic: title
characteristics.text: pBK-CMV
characteristics.characteristic: vector