characteristics.text: SAMN00163139
characteristics.characteristic: External Id
characteristics.text: LIBEST_008613
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 2001-01-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:11.733Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized; constructed in the laboratory of M. Bento Soares (University of Iowa).
characteristics.characteristic: description
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: lymphocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000542
characteristics.characteristic: tissue-type
characteristics.text: Soares_NPBMC
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector