characteristics.text: SAMN00154337
characteristics.characteristic: External Id
characteristics.text: LIBEST_019947
characteristics.characteristic: External Id
characteristics.text: Instituto Butantan
characteristics.characteristic: INSDC center name
characteristics.text: 2006-07-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:32.713Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 7ug of mRNA from Philodryas olfersii venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (350-600 pb and up 600 pb) in agarose gel electrophoresis, linked to Sal I adapters and directionally cloned in pSPORT-1 vector. ESTs were generated from random clones and grouped in clusters of unique sequences.
characteristics.characteristic: description
characteristics.text: venom glands
characteristics.characteristic: organ
characteristics.text: Philodryas olfersii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_120305
characteristics.characteristic: organism
characteristics.text: Sal I
characteristics.characteristic: re_1
characteristics.text: Not I
characteristics.characteristic: re_2
characteristics.text: venom glands
characteristics.characteristic: tissue-type
characteristics.text: Colubridae snake Philodryas olfersii cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT-1
characteristics.characteristic: vector