characteristics.text: SAMN00163429
characteristics.characteristic: External Id
characteristics.text: LIBEST_008915
characteristics.characteristic: External Id
characteristics.text: Stanford University
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:20.530Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: BMS (Black Mexican Sweet)
characteristics.characteristic: cultivar
characteristics.text: The library was prepared by George Rudenko using poly (A) selected RNA and Universal Riboclone cDNA Synthesis System (Promega). cDNA was synthesized using both random and oligo(dT) primers in separate reactions and equipped with EcoRI adaptors. Library was size-fractionated on agarose gels (for insert size >400bp) and non-directionally cloned into EcoRI-digested pUC19 vector. Blue/white selection on carbenicillin-containing plates was used to recover positive clones.
characteristics.characteristic: description
characteristics.text: mixed logarithmic and stationary growth phases
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: suspension culture
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000221
characteristics.characteristic: tissue-type
characteristics.text: 951 - BMS tissue from Walbot Lab (GR)
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pUC19
characteristics.characteristic: vector