characteristics.text: SAMD00012484
characteristics.characteristic: External Id
characteristics.text: Genomics Unit, Center of Molecular Biosciences, Tropical Biosphere Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-11-12T03:29:29Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS002677
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS002677
characteristics.characteristic: SRA accession
characteristics.text: Gordonia rubripertincta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_36822
characteristics.characteristic: organism
characteristics.text: Bacteria and growth conditions: Cultures of R. corallinus B-276 (ATCC31338) were grown on Nutrient Broth No. 2 (Oxoid, Basingstoke, Hampshire, U.K.) containing 1% (w/v) glucose (NBG) at 30 Â°C with aerobic shaking (NSC). For the SC, propene and carbon were supplied via the gas phase with incubation in the NBG media at 30 Â°C. Cultures were grown either in shaking flasks or in sealed shaking flasks with propene (10%, v/v) supplied via the gas phase. Cells were harvested by centrifugation (8,000 Ã g for 10 min), washed twice with sterilized water, followed by re-centrifugation (8,000 Ã g for 10 min) to obtain cell pellets for DNA extraction. Cell pellets were stored at −80Â°C until use.
characteristics.characteristic: sample comment
characteristics.text: DRS002677
characteristics.characteristic: sample name
characteristics.text: B-276
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000236
characteristics.characteristic: strain
characteristics.text: Rhodococcus corallinus strain B-276 (NSC)
characteristics.characteristic: title