characteristics.text: SAMN00155973
characteristics.characteristic: External Id
characteristics.text: LIBEST_001391
characteristics.characteristic: External Id
characteristics.text: Life Science College of Wuhan University
characteristics.characteristic: INSDC center name
characteristics.text: 1998-08-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:39.380Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer; double-stranded cDNA was ligated to Sal I adaptors. After digestion with Not I, the cDNA was ligated into Sal I-Not I-cut pSPORT1 plasmid and electroporated into high-efficency electrocompetent coli DH10B (Life Technologies) using the Bio-Rad GenePulser. Library has an average insert length of 1.6 kb. Library constructed by Deming Gou, Huang Jian, Li Wenxin.
characteristics.characteristic: description
characteristics.text: About 3-week-old embryo after fertilization
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Sal I
characteristics.characteristic: re_2
characteristics.text: Whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: tissue-type
characteristics.text: human embryo cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT1
characteristics.characteristic: vector