characteristics.text: SAMN00164699
characteristics.characteristic: External Id
characteristics.text: LIBEST_022461
characteristics.characteristic: External Id
characteristics.text: Dipartimento di Ortofloroarboricoltura e Tecnologie Agroalimentari, Sezione Arboricoltura, University of Catania,
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:45.590Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted using Plant RNA Reagent (Invitrogen) from flowers at different developmental stages, sampled from three genotypes differing for sex (male, female, hermaphrodite). Equal amount of total RNAs were bulked before mRNA isolation. mRNA was isolated by Oligotex mRNA mini Kit (Qiagen). A cDNA library was constructed using cDNA Synthesis Kit and Uni-ZAP XR vector (Stratagene) following the manufacturer's instructions. After the excision of pBluescript SK(-) phagemid from the Uni-ZAP XR through ExAssist helper phage, and the infection of E. coli SOLR cells, the colonies were plated on LB medium with 100 ug/ml ampicillin. Clones were randomly picked for bidirectional sequencing with T3 and T7 primers. Clones were sequenced using an ABI 3730 XL sequencer (Applied Biosystems) at the Institute for Integrative Genome Biology, Core Instrumentation Facilities (CIF), University of California, Riverside (UCR).
characteristics.characteristic: description
characteristics.text: E. coli SOLR
characteristics.characteristic: lab-host
characteristics.text: Ceratonia siliqua
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_20340
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Ceratonia siliqua flowers cDNA library
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda UNi-ZAP XR
characteristics.characteristic: vector