characteristics.text: LIBEST_023936
characteristics.characteristic: External Id
characteristics.text: SAMN00166897
characteristics.characteristic: External Id
characteristics.text: University of Bern
characteristics.characteristic: INSDC center name
characteristics.text: 2010-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:32.067Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Laboratory strain. Rosetting rate N/A. Ring stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.
characteristics.characteristic: description
characteristics.text: Ring
characteristics.characteristic: dev-stage
characteristics.text: 3D7AH1
characteristics.characteristic: isolate
characteristics.text: Human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: lab-host
characteristics.text: Plasmodium falciparum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5833
characteristics.characteristic: organism
characteristics.text: Blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: tissue-type
characteristics.text: Laboratory isolate 3D7AH1 Pf Ring stage var gene cDNA
characteristics.characteristic: title
characteristics.text: pcrII-TOPO
characteristics.characteristic: vector