characteristics.text: LIBEST_023058
characteristics.characteristic: External Id
characteristics.text: SAMN00165627
characteristics.characteristic: External Id
characteristics.text: University of Agricultural Science, GKVK, Bangalore
characteristics.characteristic: INSDC center name
characteristics.text: 2008-05-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:03.547Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cracking type
characteristics.characteristic: cultivar
characteristics.text: PCR product having unique band between cracking and non- Cracking genotype of Jack was reamplified with the primer having EcoRI sites having flanking the amplified region. The PCR product was cloned in pUC18 (Acc No: L09136) at EcoRI site and insert was Confirmed by EcoRI digestion and the positive clone was sequenced. Cloned using Quick LIgation Kit (Chromous Cat# RKT31). Plasmid used was pUC18 (AccNo. L09136). Sequencing was done in Sequencing Laboratory, Chromous Biotech Pvt. Ltd. Bangalore-65, India.
characteristics.characteristic: description
characteristics.text: Young leaves
characteristics.characteristic: organ
characteristics.text: Artocarpus heterophyllus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3489
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: Young leaves
characteristics.characteristic: tissue-type
characteristics.text: Jackfruit cv. cracking type induced gene
characteristics.characteristic: title
characteristics.text: pUC18
characteristics.characteristic: vector