characteristics.text: SAMN00163309
characteristics.characteristic: External Id
characteristics.text: LIBEST_008787
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-Food Canada - SCPFRC
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:17.363Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Landrace
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0000692
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from polyA+ enriched mRNA from field grown leaves. Mid-size actively growing leaves were collected and pooled from several plants and frozen immediately after harvesting in liquid nitrogen. The cDNA was prepared using an XhoI-poly(dT) linker-primer. An EcoRI adapter was ligated to the blunt end cDNA and the products were digested with EcoRI and XhoI enabling directional cloning into the lambda ZAP Express vector. The library was amplified using the host strain XL1-Blue MRF'. Mass excision of the library was performed to obtain pBK-CMV phagemid clones in the host strain XLOLR. Single pass DNA sequencing was performed using the T3 promoter primer: 5' ATTAACCCTCACTAAAGGGA 3'. This library was constructed by Alex Richman.
characteristics.characteristic: description
characteristics.text: field grown, mid-size
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Stevia rebaudiana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_55670
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: 751/1501
characteristics.characteristic: strain
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Stevia field grown leaf cDNA
characteristics.characteristic: title
characteristics.text: ds phagemid
characteristics.characteristic: v_type
characteristics.text: pBK-CMV
characteristics.characteristic: vector