characteristics.text: LIBEST_022831
characteristics.characteristic: External Id
characteristics.text: SAMN00165375
characteristics.characteristic: External Id
characteristics.text: University of Missouri, National Center for Soybean Biotechnology, Division of Plant Sciences and Biochemistry
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:56.050Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Early gallatin
characteristics.characteristic: cultivar
characteristics.text: ESTs were sequenced from a normalized, subtracted library. mRNA was extracted from bean leaves (cv. early gallatin) infected by the bean rust pathogen (U. appendiculatus). This mRNA was subtracted against normal leaf (uninfected) RNA and from RNA from germinated U. appendiculatus spores. mRNA were extracted from plants infected with either a virulent fungal strain, race41, or an avirulent strain, race49. The RNA was extracted from leaves 0-6-12-15-24-48-72-96-120 hours after inoculation. The resulting cDNA was pooled into early (0-24 h) and late (48-120 h) fractions and tagged during cDNA preparation so that they could be placed into the early and late fractions after sequencing. The cDNA was cloned into pGEMT and sequenced with M13 primer.
characteristics.characteristic: description
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organ
characteristics.text: Phaseolus vulgaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3885
characteristics.characteristic: organism
characteristics.text: Phaseolus vulgaris cv Early gallatin
characteristics.characteristic: title
characteristics.text: pGEMT
characteristics.characteristic: vector