characteristics.text: SAMN00164195
characteristics.characteristic: External Id
characteristics.text: LIBEST_009688
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-08-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:44.327Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University
characteristics.characteristic: description
characteristics.text: enriched for 2nd stage juveniles
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Heterodera glycines
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_51029
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Heterodera glycines J2 pAMP1 v8 Chiapelli McCarter
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pAMP1 (Gibco)
characteristics.characteristic: vector