characteristics.text: SAMN00150104
characteristics.characteristic: External Id
characteristics.text: LIBEST_019474
characteristics.characteristic: External Id
characteristics.text: Xi'an Jiaotong University
characteristics.characteristic: INSDC center name
characteristics.text: 2006-04-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:19.543Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: bladder transitional cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006544
characteristics.characteristic: cell type
characteristics.text: BLX
characteristics.characteristic: cell-line
characteristics.text: A differentially expressed gene subtractive library was constructed by using subtractive suppression hybridization between the pair of bladder transitional cell lines BLX and BLS-211 with different cell shape. These EST fragments were ligated with pGEM-T vectors and amplified in JM109 E. coli. After blue-white screening, the white positive inserts were picked out and stored.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: dev-stage
characteristics.text: bladder
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001255
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: bladder transitional cell carcinoma cell lines subtractive library BLX
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector