characteristics.text: LIBEST_021017
characteristics.characteristic: External Id
characteristics.text: SAMN00152776
characteristics.characteristic: External Id
characteristics.text: Norwegian Forest Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:06.717Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Subtracted array: Tester: sample F6_12SD6 (short day, cold environment); Driver: F6_12LD6 (long day, cold environment - control) genes predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (outdoor) after short day treatment (going to bud set); Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit, Obtained arrays were size-fractionated using CHROMA SPIN-400 Columns and non-directionally cloned in a pDrive vector using PCR Cloning Kit.
characteristics.characteristic: description
characteristics.text: 1-year seedlings, growing in chambers with controlled conditions, on the 6th day after starting of short day treatment (12 hours of light and dark)
characteristics.characteristic: dev-stage
characteristics.text: Picea abies
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3329
characteristics.characteristic: organism
characteristics.text: whole green part of seedlings
characteristics.characteristic: tissue-type
characteristics.text: CC - Norway Spruce Subtracted Library (bud set - short day)
characteristics.characteristic: title
characteristics.text: pDrive vector (Qiagen)
characteristics.characteristic: vector