characteristics.text: SAMN00014756
characteristics.characteristic: External Id
characteristics.text: Joint Genome Institute, U.S. Department of Energy
characteristics.characteristic: INSDC center name
characteristics.text: 2010-06-10T15:29:16.217Z
characteristics.characteristic: INSDC first public
characteristics.text: 2016-04-07T14:33:25.250Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS074576
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS074576
characteristics.characteristic: SRA accession
characteristics.text: Chlamydomonas reinhardtii 137c anaerobic dark
characteristics.characteristic: description
characteristics.text: anaerobic,dark
characteristics.characteristic: in vivo conditions
characteristics.text: C. reinhardtii strain CC-124 cells were grown in TAP medium at 24 Celsius, 180 RPM shaking, and under continuous light between 30-60 µmol/m^2*s. Cells were grown in 6 x 1 L TAP to density of 1.7x10^6 cells/ml before being concentrated by centrifugation (5min, 2170xg). All pellets were resuspended in a total of 1 L TAP and distributed to two 500 mL bottles. Sealed bottles were kept in the dark. RNA was isolated from cells collected after 45min (bottle 1) and 120min (bottle 2). RNA samples were pooled in equal proportion for sequencing. Sample provided by Sabeeha Merchant and Anja Hemschemeier.
characteristics.characteristic: library description
characteristics.text: RAPID
characteristics.characteristic: library type
characteristics.text: Chlamydomonas reinhardtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3055
characteristics.characteristic: organism
characteristics.text: 137c
characteristics.characteristic: strain
characteristics.text: Generic sample from Chlamydomonas reinhardtii
characteristics.characteristic: title