characteristics.text: LIBEST_021164
characteristics.characteristic: External Id
characteristics.text: SAMN00152906
characteristics.characteristic: External Id
characteristics.text: Oklahoma State University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-12-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:11.210Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: IDE8
characteristics.characteristic: cell-line
characteristics.text: Total RNA was isolated from three A. marginale-infected and three uninfected IDE8 cell cultures using TriReagent (Sigma) according to manufacturer's instructions. Pools of 20 ug RNA were made from uninfected and infected tick cells. SSH was performed at Evrogen JCS (Moscow, Russia) as described previously (Naranjo et al., 2006; FEMS Immunol Med Microbiol 46: 298-312).
characteristics.characteristic: description
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: lab-host
characteristics.text: Ixodes scapularis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6945
characteristics.characteristic: organism
characteristics.text: cDNA suppression subtraction library from Anaplasma marginale infected and uninfected Ixodes scapularis IDE8 tick cells
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pAL-16
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000649
characteristics.characteristic: vector