characteristics.text: LIBEST_023635
characteristics.characteristic: External Id
characteristics.text: SAMN00166528
characteristics.characteristic: External Id
characteristics.text: Embrapa Cenargen
characteristics.characteristic: INSDC center name
characteristics.text: 2008-09-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:20.907Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: IAC 98/708
characteristics.characteristic: cultivar
characteristics.text: Total RNA was extracted from frozen root tissues using CONCERT Large-Scale RNA Isolation (Invitrogen) according to the manufacture's directions. Poly-A+ mRNA was isolated using an Oligotex mRNA Bath Protocol kit according to manufacturer's manual (QIAGEN). A cDNA library was constructed from four micrograms of poly-A+ mRNA, measured by absorbance at 260 nm, in the pSPORT 1 vector exploiting the NotI and SalI restrictions sites at the 5' and 3' ends respectively. It was used Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) according manufacturer's directions.
characteristics.characteristic: description
characteristics.text: Roots
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000989
characteristics.characteristic: organ
characteristics.text: Gossypium hirsutum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3635
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Root
characteristics.characteristic: tissue-type
characteristics.text: Cotton susceptible to Meloidogyne incognita
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pSPORT 1
characteristics.characteristic: vector