characteristics.text: LIBEST_007129
characteristics.characteristic: External Id
characteristics.text: SAMN00161662
characteristics.characteristic: External Id
characteristics.text: University of Florida
characteristics.characteristic: INSDC center name
characteristics.text: 2001-05-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:38:27.030Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from asynchronous blood stage forms of the cloned ANKA isolate of P. berghei, grown in Wistar rats to 30% parasitemia and 2-5% gametocytemia. Contaminating host white cells had previously been removed and final host cell contamination estimated to be approximately 5%. PolyA+ RNA was extracted and reverse transcribed using an oligo dT-XhoI primer (Lambda ZAP II cDNA cloning kit, Stratagene). Second strand cDNA was made following the manufacturer's protocol. EcoRI adaptors were ligated to the cDNA, and fragments were ligated into EcoRI/XhoI digested vector.
characteristics.characteristic: description
characteristics.text: asynchronous blood stage
characteristics.characteristic: dev-stage
characteristics.text: Wistar rats
characteristics.characteristic: lab-host
characteristics.text: Plasmodium berghei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5821
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: ANKA clone HP (gametocyte producer)
characteristics.characteristic: strain
characteristics.text: Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali
characteristics.characteristic: title
characteristics.text: phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II vector DNA, excised from Lamda ZAP II.
characteristics.characteristic: vector