characteristics.text: SAMN00156238
characteristics.characteristic: External Id
characteristics.text: LIBEST_001664
characteristics.characteristic: External Id
characteristics.text: 2014-02-03T23:44:07.523Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:21:56.060Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTAAC 3' and 5' GTTACTAGTG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.
characteristics.characteristic: description
characteristics.text: adult, age 27
characteristics.characteristic: dev-stage
characteristics.text: DH10B (phage resistant)
characteristics.characteristic: lab-host
characteristics.text: muscle (psoas)
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001298
characteristics.characteristic: organ
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Barstead muscle HPLRB8
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector