characteristics.text: LIBEST_001726
characteristics.characteristic: External Id
characteristics.text: SAMN00156298
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:48.807Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Templates for the differential-display RT-PCR were reverse-transcribed from total cellular RNA extracted (1) from excised feeding sites of roots with parasitic nematodes attached to it and (2) from adjacent root pieces of the same size supplemented with non-parasitic nematodes. Expression profiles were compared between (1) and (2) and cDNAs displaying differential expression were cloned into the pCR2.1-TOPO TA-vector (Invitrogen).
characteristics.characteristic: description
characteristics.text: pre-parasitic, second-stage, and third-stage parasitic juveniles
characteristics.characteristic: dev-stage
characteristics.text: Heterodera schachtii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_97005
characteristics.characteristic: organism
characteristics.text: Sugar-beet cyst nematodes (Heterodera schachtii)
characteristics.characteristic: tissue-type
characteristics.text: Cloned cDNAs of Heterodera schachtii generated by mRNA differential-display RT-PCR
characteristics.characteristic: title