characteristics.text: SAMN00149827
characteristics.characteristic: External Id
characteristics.text: LIBEST_019152
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2006-02-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:11.283Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Geneva 3041
characteristics.characteristic: cultivar
characteristics.text: Total RNA was extracted separately from whole root using the 'pin tree' method(Gasic et.al,2004). Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit(Qiagen). MRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TGCTGG}]. Double stranded cDNAs were size selected(more than 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBS II SK+ phagemid vector (Stratagent). The total number of white colony forming units was 1.2x10^6 cfu. The background of empty clones was less than 10%. Inserts ranged from 0.5kb to 3kb as determined by PCR.
characteristics.characteristic: description
characteristics.text: Tissue challenged with Erwinia
characteristics.characteristic: dev-stage
characteristics.text: DH10B cells
characteristics.characteristic: lab-host
characteristics.text: Malus hybrid rootstock
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_370305
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Apple_EST_Mdfbg
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: pBluescript II SK (+)
characteristics.characteristic: vector