characteristics.text: SAMN00154160
characteristics.characteristic: External Id
characteristics.text: LIBEST_016822
characteristics.characteristic: External Id
characteristics.text: University of Maryland
characteristics.characteristic: INSDC center name
characteristics.text: 2005-01-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:04.160Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: INRA clone 717 1-B4 and ethylene insensitive line R1-20
characteristics.characteristic: cultivar
characteristics.text: Developing vegetative apical buds were collected after various time intervals of SD treatment. Total RNA was isolated and used for cDNA-AFLP profiling with ApoI and Mse1. RNA was pooled for buds representing initiation (1, 2,3 weeks of SD treatment), development (4,5,6 weeks of SD treatment), maturation and dormancy (7 and 8 weeks of SD treatment) and dormancy (9 and 12 weeks SD with low temperature). Transcript derived fragments (TFDs) that showed differential abundance during bud development and dormancy or between genotypes was excised from the cDNA-AFLP gel, amplified by PCR and TA cloned into either pGEM-T or pGEM-T Easy. Cloned TFDs were then sequenced using sequencing primers. The project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number # (2003-35304-13161).
characteristics.characteristic: description
characteristics.text: Initiated, maturing and dormant
characteristics.characteristic: dev-stage
characteristics.text: Developing vegetative bud
characteristics.characteristic: organ
characteristics.text: Populus tremula x Populus alba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_80863
characteristics.characteristic: organism
characteristics.text: Populus tremula X Populus alba developing vegetative bud cDNA Library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T or pGEM-T Easy
characteristics.characteristic: vector