characteristics.text: SAMN00151955
characteristics.characteristic: External Id
characteristics.text: LIBGSS_001317
characteristics.characteristic: External Id
characteristics.text: Virginia Commonwealth University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-02-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-10T15:39:10.203Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Sau3AI partial digestion of NEMC1 genomic DNA was ligated into lambda Zap Express BamHI arms treated with calf intestine alkaline phosphatase. Plasmids were excised as directed by the manufacturer, purified by a modified alkaline lysis protocol and sequenced using PEB BigDye terminators on an PEB Prism 377 DNA Sequencer.
characteristics.characteristic: description
characteristics.text: Oocyst
characteristics.characteristic: dev-stage
characteristics.text: gnotobiotically reared piglet
characteristics.characteristic: lab-host
characteristics.text: Cryptosporidium parvum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5807
characteristics.characteristic: organism
characteristics.text: BamHI
characteristics.characteristic: re_1
characteristics.text: NEMC1, genotype 1 (human)
characteristics.characteristic: strain
characteristics.text: C. parvum Lambda Zap Express Library
characteristics.characteristic: title
characteristics.text: Lambda Zap Express (Stratagene)
characteristics.characteristic: vector