characteristics.text: SAMN00155247
characteristics.characteristic: External Id
characteristics.text: LIBEST_000648
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 1997-04-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T14:20:32.213Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The cDNA library was constructed by Cindy Tripp (Heska Corporation, 1825 Sharp Point Drive, Fort Collins, Colorado 80525, USA) From 200,000 larval stage T. canis, using a single step guanadine-phenol-chloroform extraction, 265 ug total RNA was recovered, from which 6 ug poly-A+ RNA was isolated by oligo-dT chromatography. cDNA synthesised from this mRNA was unidirectionally cloned into the Uni-Zap XR phage vector, using packaging extracts from Stratagene. The amplified library contained 1.9 x 109 phage/ml with 91% recombinants.
characteristics.characteristic: description
characteristics.text: Toxocara canis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6265
characteristics.characteristic: organism
characteristics.text: Toxocara canis infective larva cDNA library
characteristics.characteristic: title