characteristics.text: SAMN00152152
characteristics.characteristic: External Id
characteristics.text: LIBEST_020426
characteristics.characteristic: External Id
characteristics.text: Friedrich-Alexander University Erlangen-Nurnberg
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:48.593Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Solara
characteristics.characteristic: cultivar
characteristics.text: sprouting tuber buds were excised from potato tubers, PolyA-RNA was isolated and used to create a cDNA-library by means of by means of the 'SMART cDNA library construction Kit' (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de
characteristics.characteristic: description
characteristics.text: potato tubers after ca. 3 months of storage, sprouting buds (ca. 2mm length)
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1-blue
characteristics.characteristic: lab-host
characteristics.text: Solanum tuberosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4113
characteristics.characteristic: organism
characteristics.text: NotI (GCGGCCGC)
characteristics.characteristic: re_1
characteristics.text: NotI (GCGGCCGC)
characteristics.characteristic: re_2
characteristics.text: potato tuber
characteristics.characteristic: tissue-type
characteristics.text: SSBN
characteristics.characteristic: title
characteristics.text: pCR-Blunt (Invitrogen)
characteristics.characteristic: vector