characteristics.text: SAMN00138959
characteristics.characteristic: External Id
characteristics.text: University of Sheffield
characteristics.characteristic: INSDC center name
characteristics.text: 2010-12-08T10:02:41.720Z
characteristics.characteristic: INSDC first public
characteristics.text: 2014-03-28T17:13:20.717Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS139748
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS139748
characteristics.characteristic: SRA accession
characteristics.text: Ten unrelated great tit fledglings were sampled from across the Wytham Woods, UK, population and brain, heart, kidney, liver, muscle, pancreas, skin and testes/ovaries dissected from each individual at between three and 23 minutes after death. Each chick was sexed at sampling and sex was later confirmed molecularly with the Z-002A marker. Tissues were stored in RNALater (Ambion) to prevent RNA degradation. RNA was extracted using the TRIzol* (Invitrogen) method. RNA quantity and degradation was tested using a Nanodrop (Agilent) with each tissue yielding a mean (SE) of 44.4 (7.5) ug of RNA with mean (SE) RNA integrity number (RIN) of 8.37 (0.21). For each tissue 1ug of RNA from each bird was pooled, i.e. to make 8 pools, each containing 10ug of RNA.
characteristics.characteristic: description
characteristics.text: Parus major
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9157
characteristics.characteristic: organism
characteristics.text: Parus major transcriptome sequencing
characteristics.characteristic: title