characteristics.text: SAMN00006255
characteristics.characteristic: External Id
characteristics.text: Oregon St Univ - Center for Genome Research & Biocomputing
characteristics.characteristic: INSDC center name
characteristics.text: 2010-01-08T12:55:46.400Z
characteristics.characteristic: INSDC first public
characteristics.text: 2013-06-12T08:52:09.987Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS008429
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS008429
characteristics.characteristic: SRA accession
characteristics.text: Pinus thunbergii THUN05
characteristics.characteristic: barcode AAT
characteristics.text: Pinus lambertiana S LAMB01
characteristics.characteristic: barcode CCCT
characteristics.text: Pinus taeda TAED06
characteristics.characteristic: barcode CGT
characteristics.text: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
characteristics.characteristic: description
characteristics.text: Pinus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3337
characteristics.characteristic: organism
characteristics.text: Generic sample from Pinus
characteristics.characteristic: title