characteristics.text: SAMN00166099
characteristics.characteristic: External Id
characteristics.text: LIBEST_015766
characteristics.characteristic: External Id
characteristics.text: Microbial Ecology, Institution of Ecology, Lund University
characteristics.characteristic: INSDC center name
characteristics.text: 2004-05-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:42:32.987Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: This EST clone is originating from one of four cDNA libraries. These libraries were constructed for transcript profiling of nematophagous ascomycete Monacrosporium haptotylum in interaction with Caenorhabditis elegans. The library 'MYCELIUM' is constructed from vegetative mycelium in a saprophytic stage, the second library 'KNOB' is constructed from the separated infection structure and finally the '4h-POST INFECTION' and the '24h-POST INFECTION' libraries represent early (4h) and late (24h) post-infection stages. These libraries where analayzed in parallel and 1729 (MYCELIUM), 1450 (KNOB), 1195 (4h-POST INFECTION) and 2741 (24h-POST INFECTION) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)pTriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli XL1-Blue). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104).
characteristics.characteristic: description
characteristics.text: 4h infection
characteristics.characteristic: dev-stage
characteristics.text: Escherichia coli XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: Caenorhabditis elegans/Monacrosporium haptotylum mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_279692
characteristics.characteristic: organism
characteristics.text: SfiI
characteristics.characteristic: re_1
characteristics.text: SfiI
characteristics.characteristic: re_2
characteristics.text: CBS 200.50/N2
characteristics.characteristic: strain
characteristics.text: Fungal and nematode tissue after 4h of infection
characteristics.characteristic: tissue-type
characteristics.text: 4h-POST INFECTION
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pTriplEx2
characteristics.characteristic: vector