characteristics.text: SAMN00152712
characteristics.characteristic: External Id
characteristics.text: LIBEST_020956
characteristics.characteristic: External Id
characteristics.text: UC Davis, Department of Pomology
characteristics.characteristic: INSDC center name
characteristics.text: 2007-03-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:05.120Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: On March 11, 2006, carrot discs were inoculated with Pratylenchus vulnus from an in vitro culture on walnut roots. On April 28, 2006, these nematodes were harvested by soaking the carrot discs in 1/4 strength M9 media for 3 hours. Nematodes were packed by centrifugation and total RNA was immediately isolated (TriZol reagent; Invitrogen CA). Total RNA was purified (Qiagen RNA/DNA Mini Kit) and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen, CA) to obtain individual colonies that were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).
characteristics.characteristic: description
characteristics.text: Mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: dev-stage
characteristics.text: E. coli DH10-Beta
characteristics.characteristic: lab-host
characteristics.text: Pratylenchus vulnus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_45931
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: PV-02
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector