characteristics.text: LIBEST_020666
characteristics.characteristic: External Id
characteristics.text: SAMN00152461
characteristics.characteristic: External Id
characteristics.text: IFAPA-CICE, Junta de Andalucia
characteristics.characteristic: INSDC center name
characteristics.text: 2007-09-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:56.340Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SA4087
characteristics.characteristic: cultivar
characteristics.text: Total RNA was isoltated from roots harvested 15, 21 and 35 days after inoculation with O. crenata. cDNA was obtained from total RNA using the SMART PCR cDNA system (Clontech). This cDNA was used as tester probe in Supression Substractive Hybridization (SSH) according to the manufacture's instructions. The SSH-cDNA fragments were ligated into the pGEM-T easy vector (Promega). Plasmids containing cDNA inserts were cloned in E. coli XL1-Blue MRF' cells (Stratagene)
characteristics.characteristic: description
characteristics.text: 15, 21 and 35 days after inoculation with Orobanche crenata
characteristics.characteristic: dev-stage
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: tissue-type
characteristics.text: MTOROCRE
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T easy
characteristics.characteristic: vector