characteristics.text: LIBEST_017373
characteristics.characteristic: External Id
characteristics.text: SAMN00154216
characteristics.characteristic: External Id
characteristics.text: Universite de Montreal
characteristics.characteristic: INSDC center name
characteristics.text: 2005-06-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:43:22.993Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Ovules were isolated from a compatible cross between self-incompatible accessions having the S12S14 self-incompatibility alleles (G4 line as female) and S11S13 self-incompatibility alleles (V22 line as male). Those two lines were derived from the parental lines PI 458314 (which carries the S11 and S12 self-incompatibility alleles) and line PI 230582 (which carries the S13 and S14 alleles) obtained through the Potato Introduction Station (Sturgeon Bay, WI). Plants were grown in greenhouses under natural light, fruit collection took place in the morning during summer (approx. 14h daylight), and fruits were pooled from different plants. Total RNA was isolated as described in EMBO J. 4: 2411-2418, 1985. The poly A+ RNA was purified from the total RNA by affinity chromatography using oligo dT agarose beads type VII (Amersham). Mean clone length of the library was 1,5 kb.
characteristics.characteristic: description
characteristics.text: Globular to walking stick embryo
characteristics.characteristic: dev-stage
characteristics.text: SOLR
characteristics.characteristic: lab-host
characteristics.text: Ovule (hand dissected)
characteristics.characteristic: organ
characteristics.text: Solanum chacoense
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4108
characteristics.characteristic: organism
characteristics.text: EcoR1
characteristics.characteristic: re_1
characteristics.text: Xho1
characteristics.characteristic: re_2
characteristics.text: 7 to 17 days post-pollination ovules
characteristics.characteristic: tissue-type
characteristics.text: SV5
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBS SK
characteristics.characteristic: vector