characteristics.text: SAMN00166364
characteristics.characteristic: External Id
characteristics.text: LIBEST_023470
characteristics.characteristic: External Id
characteristics.text: Austrian Research Centers GmbH - ARC
characteristics.characteristic: INSDC center name
characteristics.text: 2008-07-30T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:15.810Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Idared
characteristics.characteristic: cultivar
characteristics.text: Total RNA was prepared from whole in-vitro plants and used as a template for amlification of cDNAs and suppression subtractive hybridization (SSH)(Clontech?). The obtained clones were spotted onto glass slides coated with g-amino- propyl-silane (GAPS II; Corning Inc., Corning, NY, USA). Arrays were co-hybridized with labelled cDNAs from control and Erwinia-infected plants and subsequently analysed. Differentially expressed clones identified were sequenced from both directions (M13 primers). Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.
characteristics.characteristic: description
characteristics.text: E. coli TOP10 (Invitrogen)
characteristics.characteristic: lab-host
characteristics.text: whole plant
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organ
characteristics.text: Malus domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3750
characteristics.characteristic: organism
characteristics.text: monoecious
characteristics.characteristic: sex
characteristics.text: stem and leaves
characteristics.characteristic: tissue-type
characteristics.text: Subtraction suppressive hybridization library ID Forward
characteristics.characteristic: title
characteristics.text: TOPO-TA (Invitrogen)
characteristics.characteristic: vector