characteristics.text: SAMN00165594
characteristics.characteristic: External Id
characteristics.text: LIBEST_023029
characteristics.characteristic: External Id
characteristics.text: Embrapa Recursos Geneticos e Biotecnologia
characteristics.characteristic: INSDC center name
characteristics.text: 2008-04-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:02.870Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: IRAT 20
characteristics.characteristic: cultivar
characteristics.text: A subtractive cDNA library of root tissue was constructed using the PCR Select Subtraction Kit (Clontech, USA). Subtractive hybridizations were performed using cDNA from the stressed plant roots (tester) against cDNA from well watered plant roots (driver). The differential products obtained were cloned into pGEM T-Easy (Promega) and sequenced in ABI Prism 3700 DNA Analyser (Applied Biosystems Inc.). A minimum insert size of 30 pb and at least 20 pb with quality of phred> 20 were considered for the analysis.
characteristics.characteristic: description
characteristics.text: Reproductive stage
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL1 Blue
characteristics.characteristic: lab-host
characteristics.text: root
characteristics.characteristic: organ
characteristics.text: Oryza sativa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4530
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: SpeI
characteristics.characteristic: re_2
characteristics.text: IRAT 20 drought stressed roots (RAIRAT)
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T Easy Vector
characteristics.characteristic: vector