characteristics.text: LIBEST_021212
characteristics.characteristic: External Id
characteristics.text: SAMN00152953
characteristics.characteristic: External Id
characteristics.text: Nagasaki University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:12.430Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Rotifers were harvested by filtration onto a plankton net (45 micron mesh) that was washed with sterilized artificial seawater; rotifers were resuspended in 400 ml seawater that was exchanged every 2-3 hours over 12 hours to allow the rotifers to consume any remaining Chlorella and excrete their gut contents. The washed and starved rotifers were collected by plankton net and suspended in ISOGEN (Nippon Gene, Japan). The rotifers were homogenized with 0.8 mm glass beads using a vortex mixer and total RNA was isolated from the homogenate according to the manufacturer.s instructions. Poly(A)+ RNA was purified from total RNA using MagExtractor (Toyobo, Japan). About 2.8 ug of PolyA RNA was used in first strand synthesis using a cDNA Synthesis Kit (Amersham Bioscience, USA). The blunt-ended cDNA was ligated to the EcoRI/NotI adaptor, then ligated into EcoRI-predigested lambda-ZAPII arms (Stratagene, USA), and packaged in vitro by using Gigapack III gold packaging extract (Stratagene).
characteristics.characteristic: description
characteristics.text: All stages represented
characteristics.characteristic: dev-stage
characteristics.text: NH1L
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006777
characteristics.characteristic: isolate
characteristics.text: Brachionus plicatilis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10195
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Population of mictic and amictic females and males
characteristics.characteristic: sex
characteristics.text: All tissues, including resting eggs
characteristics.characteristic: tissue-type
characteristics.text: Brachionus plicatilis NHIL unnormalized cDNA library
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBluescript SK(-)
characteristics.characteristic: vector