characteristics.text: SAMN00152789
characteristics.characteristic: External Id
characteristics.text: LIBEST_021031
characteristics.characteristic: External Id
characteristics.text: The Plant Biotechnology Institute, National Research Council Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:07.110Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Topas (embryogenic line DH4079)
characteristics.characteristic: cultivar
characteristics.text: Embryogenesis was induced in the microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Following heat stress, cultures were transferred to 24 degrees C for an additional 2d. These 5d cultures contained embryogenic as well as some pollen-like structures.
characteristics.characteristic: description
characteristics.text: 5d cultures; 3d of 32 degrees C heat stress, then 2d at 24 degrees C
characteristics.characteristic: dev-stage
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: Sfi 1A
characteristics.characteristic: re_1
characteristics.text: Sfi 1B
characteristics.characteristic: re_2
characteristics.text: Microspore
characteristics.characteristic: tissue-type
characteristics.text: 44JK5DM
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector