characteristics.text: LIBEST_021039
characteristics.characteristic: External Id
characteristics.text: SAMN00152794
characteristics.characteristic: External Id
characteristics.text: Okazaki National Research Institutes
characteristics.characteristic: INSDC center name
characteristics.text: 2007-04-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:07.293Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Malaria parasites harboring GFP driven by the hsp70 promoter were used (see Ishino et al. Mol Microbiol. 2006;59(4):1175-84.). Maralia parasites (hsp70:GFP) infected hepatocytes were collected from female wistar rats by perfusion 31 h after sporozoites inoculation, and were isolated by cell-sorting. Total RNAs were extracted using RNAgents Total RNA Isolation System (Promega). Directional cDNA libraries were constructed into pDNR-LIB vector by PCR-based method using Creator Smart cDNA library Construction Kit (BD Bioscience).
characteristics.characteristic: description
characteristics.text: liver stage parasite
characteristics.characteristic: dev-stage
characteristics.text: Plasmodium berghei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5821
characteristics.characteristic: organism
characteristics.text: Pb(hsp70:GFP)
characteristics.characteristic: strain
characteristics.text: Plasmodium berghei liver stage parasite
characteristics.characteristic: title
characteristics.text: pDNR_LIB (Clontech)
characteristics.characteristic: vector