characteristics.text: SAMN00163186
characteristics.characteristic: External Id
characteristics.text: LIBEST_008660
characteristics.characteristic: External Id
characteristics.text: Washington University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2001-02-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:13.190Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: The library was constructed using mRNA isolated from total RNA with oligo-dT cellulose. Total RNA was generated from muscle larvae that were isolated from infected rats. Larvae were liberated by pepsin/HCl digestion, incubated with 1% SDS, treated with RNase and DNase to eliminate host nucleic acid contamination, and purified on a Percoll gradient. The T. spiralis isolate was obtained from Dickson Despommier (Columbia University). The library was provided by Dr. Doug Jasmer (djasmer@vetmed.wsu.edu) and colleagues at Washington State University. DNA Sequencing by: Washington University Genome Sequencing Center St. Louis.
characteristics.characteristic: description
characteristics.text: muscle stage larvae
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Trichinella spiralis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6334
characteristics.characteristic: organism
characteristics.text: NotI
characteristics.characteristic: re_1
characteristics.text: SalI
characteristics.characteristic: re_2
characteristics.text: Trichinella spiralis ML CMVsport jasmer
characteristics.characteristic: title
characteristics.text: plasmid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pCMVsport-7.neo
characteristics.characteristic: vector