characteristics.text: SAMN00157148
characteristics.characteristic: External Id
characteristics.text: LIBEST_002589
characteristics.characteristic: External Id
characteristics.text: The Institute of Physical and Chemical Research (RIKEN)
characteristics.characteristic: INSDC center name
characteristics.text: 1999-11-12T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:36:11.637Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI
characteristics.characteristic: description
characteristics.text: egg
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000369
characteristics.characteristic: dev-stage
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: BamHI
characteristics.characteristic: re_2
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: in vitro fertilized eggs
characteristics.characteristic: tissue-type
characteristics.text: RIKEN full-length enriched, in vitro fertilized eggs
characteristics.characteristic: title