characteristics.text: LIBEST_005582
characteristics.characteristic: External Id
characteristics.text: SAMN00160120
characteristics.characteristic: External Id
characteristics.text: Agriculture and Agri-Food Canada
characteristics.characteristic: INSDC center name
characteristics.text: 2000-08-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:37:36.880Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Glycine max cultivar Harosoy
characteristics.characteristic: cultivar
characteristics.text: This cDNA library was constructed from polyA+ enriched mRNA from etiolated hypocotyls 48 h post-inoculation with Phytophthora sojae zoospores. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.
characteristics.characteristic: description
characteristics.text: 8 d old etiolated hypocotyls 48 h post-inoculation with Phytophthora sojae zoospores
characteristics.characteristic: dev-stage
characteristics.text: E. coli strain XLOLR
characteristics.characteristic: lab-host
characteristics.text: Glycine max/Phytophthora sojae mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_135715
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Phytophthora sojae race 2 strain P6497
characteristics.characteristic: strain
characteristics.text: Plant hypocotyls infected with Phytophthora sojae
characteristics.characteristic: tissue-type
characteristics.text: PsojaeHA
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBK-CMV
characteristics.characteristic: vector