characteristics.text: LIBGSS_003036
characteristics.characteristic: External Id
characteristics.text: SAMN00152057
characteristics.characteristic: External Id
characteristics.text: University of Florida
characteristics.characteristic: INSDC center name
characteristics.text: 2000-12-04T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-05-20T13:20:27.453Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Genomic DNA, from asynchronous blood stage parasites of the cloned Honduran HB3 isolate cultured in vitro, was digested with mung bean nuclease in the presence of 30% formamide at 50oC (Vernick, K.D., Imberski, R.B., and McCutchan, T.F. 1988. Nucleic Acids Research 16:6883-6896). The ends of the fragments were polished using T4 DNA polymerase, and the fragments were ligated to EcoR V-cleaved and dephosphorylated pBlueScript SK(+). Recombinant plasmids transformed E. coli XL1-Blue.
characteristics.characteristic: description
characteristics.text: E. coli XL1-Blue
characteristics.characteristic: lab-host
characteristics.text: Plasmodium falciparum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5833
characteristics.characteristic: organism
characteristics.text: gmbPfHB3.1, G. Roman Reddy
characteristics.characteristic: title
characteristics.text: pBlueScript SK(+)
characteristics.characteristic: vector