characteristics.text: callus derived from needle tissue
characteristics.characteristic: BODYSITE
characteristics.text: SAMN00102755
characteristics.characteristic: External Id
characteristics.text: Joint Genome Institute, U.S. Department of Energy
characteristics.characteristic: INSDC center name
characteristics.text: 2010-08-12T13:09:05.240Z
characteristics.characteristic: INSDC first public
characteristics.text: 2015-11-25T12:20:43.897Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS107347
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS107347
characteristics.characteristic: SRA accession
characteristics.text: Pinus taeda callus
characteristics.characteristic: description
characteristics.text: Callus cells were grown in liquid medium (100 mL cultures) to a point where maximum cell density was obtained- 14 days. Elicitors were dissolved in culture medium and added to each culture to give the final concentrations listed below. Cultures were then incubated for another 4 hr. Following cell harvest and RNA isolation, equivalent amounts of total RNA were pooled and the pooled sample was treated with Ambion RNase free DNAse. 1. Methyl Jasmonate (100uM) 2. Gibbberellic Acid (100uM) 3. Kinetin (100uM) 4. Cycloheximide (0.5mg/mL) 5. Indole-3-acetic Acid (100uM) 6. Nitrophenol (100uM) 7. Sodium Azide (100uM) 8. Colchicine (100uM) 9. Sodium (meta) vanadate (100uM) 10. Hydrogen Peroxide (0.01%) 11. Chitin (1mg/mL) 12. Chitosan (100ug/mL) 13. Untreated Sirex noctilio venom glands (125mg/mL) 14. Boiled Sirex noctilio venom glands (125mg/mL)
characteristics.characteristic: library description
characteristics.text: standard
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C44275
characteristics.characteristic: library type
characteristics.text: Pinus taeda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3352
characteristics.characteristic: organism
characteristics.text: Generic sample from Pinus taeda
characteristics.characteristic: title