characteristics.text: LIBEST_022497
characteristics.characteristic: External Id
characteristics.text: SAMN00164731
characteristics.characteristic: External Id
characteristics.text: University of Georgia
characteristics.characteristic: INSDC center name
characteristics.text: 2008-01-11T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:46.500Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: National Tropical Botanic Garden_F
characteristics.characteristic: cultivar
characteristics.text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GGGTCCTGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1031; Primary Titer: 5E6 pfu total; Amplified Titer: 1.4E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).
characteristics.characteristic: description
characteristics.text: premeiotic
characteristics.characteristic: dev-stage
characteristics.text: SOLR (E. Coli)
characteristics.characteristic: lab-host
characteristics.text: premeiotic flower buds
characteristics.characteristic: organ
characteristics.text: Amborella trichopoda
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13333
characteristics.characteristic: organism
characteristics.text: Xho1
characteristics.characteristic: re_1
characteristics.text: EcoR1
characteristics.characteristic: re_2
characteristics.text: Female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: flower buds
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000056
characteristics.characteristic: tissue-type
characteristics.text: FGP Female Amborella trichopoda
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScript_SKminus
characteristics.characteristic: vector