characteristics.text: SAMN00152957
characteristics.characteristic: External Id
characteristics.text: LIBEST_021216
characteristics.characteristic: External Id
characteristics.text: Fujian Agriculture and Forestry University
characteristics.characteristic: INSDC center name
characteristics.text: 2007-05-17T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:12.513Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Minhua 6
characteristics.characteristic: cultivar
characteristics.text: The full length cDNA library was constructed from peanut cultivar Minhua 6. The peanut pod from DAP 9 to 50 were collected, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated TRNZOL-Reagent (Tangen) and purified and mRNA was extracted from total RNA by mRNA extration kit (promega). cDNA synthesis by primer extension and library construction followed the protocol of Creator SMART cDNA Library Construction Kit. The cDNA above 500bp were collected after size-fraction. The inserts were directionally cloned into pDNR-LIB vector using the asymmetrical Sfi I sites, Sfi1a and Sfi1b sites. The library was constructed by Dr. Ninbo Cai and Dr. Weijian Zhuang.
characteristics.characteristic: description
characteristics.text: 9 to 50 days after pegging
characteristics.characteristic: dev-stage
characteristics.text: DH5a
characteristics.characteristic: lab-host
characteristics.text: Pods
characteristics.characteristic: organ
characteristics.text: Arachis hypogaea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3818
characteristics.characteristic: organism
characteristics.text: Sfi1a
characteristics.characteristic: re_1
characteristics.text: Sfi1b
characteristics.characteristic: re_2
characteristics.text: Full length peanut pod cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector