characteristics.text: SAMN00164395
characteristics.characteristic: External Id
characteristics.text: LIBEST_009892
characteristics.characteristic: External Id
characteristics.text: Institut fur Pflanzenzuchtung und Pflanzenschutz
characteristics.characteristic: INSDC center name
characteristics.text: 2002-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:49.423Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Mutin
characteristics.characteristic: cultivar
characteristics.text: RNA was isolated from plants 48 and 72 h post infection with Colletotrichum graminicola. After purification of mRNA first strand cDNA synthesis was performed, and infection-specific cDNAs were obtained after subtractive hybridization with photobiotinylated mRNA from vegetative hyphae and healthy maize leaves. The infection-specific cDNAs were subjected to second strand cDNA synthesis, and these cDNAs, representing putative differentially ESTs, were cloned into pCR 4-TOPO.
characteristics.characteristic: description
characteristics.text: 48 to 72 hours post infection
characteristics.characteristic: dev-stage
characteristics.text: E. colli Top 10
characteristics.characteristic: lab-host
characteristics.text: Zea mays/Colletotrichum graminicola mixed EST library
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_176297
characteristics.characteristic: organism
characteristics.text: CgM2
characteristics.characteristic: strain
characteristics.text: Infected leaves
characteristics.characteristic: tissue-type
characteristics.text: Interaction-specific EST library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR 4-TOPO (Topo TA cloning kit, Invitrogen)
characteristics.characteristic: vector