characteristics.text: LIBEST_009989
characteristics.characteristic: External Id
characteristics.text: SAMN00164489
characteristics.characteristic: External Id
characteristics.text: The Institute for Genomic Research
characteristics.characteristic: INSDC center name
characteristics.text: 2001-11-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:52.070Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: At 20-25% parasitemia, blood was collected from BALB/cByJ mice infected with Py17XL parasites, and leukocytes removed by passage over microcrystalline cellulose collumns. Total RNA was isolated using the guanidinium isothiocyanate method, and mRNA isolated using oligo(dT)-cellulose chromatography. First strand cDNA synthesis was completed using a 50-base primer and reverse transcriptase in the presence of 5-methyl dCTP. After second strand synthesis, uneven termini were treated with Pfu DNA polymerase and EcoRI adaptors ligated to the blunt ends. The sample was cleaved with XhoI and separated on a Sephacryl S-500 column. Size-fractionated cDNA was precipitated and ligated to HybriZAP arms directionally using EcoRI-XhoI cleaved arms. After packaging, the phagemid vector (pAD-GAL4) was excised from the HybriZAP vector and plasmid DNA isolated.
characteristics.characteristic: description
characteristics.text: Asexual blood stages
characteristics.characteristic: dev-stage
characteristics.text: E. coli XL-1 Blue
characteristics.characteristic: lab-host
characteristics.text: Plasmodium yoelii yoelii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_73239
characteristics.characteristic: organism
characteristics.text: 17XL
characteristics.characteristic: strain
characteristics.text: PyBS
characteristics.characteristic: title
characteristics.text: Yeast activation domain vector
characteristics.characteristic: v_type
characteristics.text: pAD-GAL4
characteristics.characteristic: vector