characteristics.text: SAMN00164946
characteristics.characteristic: External Id
characteristics.text: LIBEST_011160
characteristics.characteristic: External Id
characteristics.text: University of Arkansas
characteristics.characteristic: INSDC center name
characteristics.text: 2002-08-28T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:23.653Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: PI560247
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_90524
characteristics.characteristic: cultivar
characteristics.text: The cDNAs were derived from reverse transcription of mRNA samples from seeds at different stages of germination and seedlings at early phase of growth under chilling stress (13oC/10oC). The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene Uni-Zap XR cDNA synthesis and library kit. A total of 150,000 phages were excised from the primary library as pBluescript phagemid clones. Enrichment of the primary excised library with chilling-induced transcripts was performed by hybridizing the primary excised library colony lifts with the PCR-select subtraction product, with cold germinated cDNA as tester and control temperature-germinated cDNA as driver.
characteristics.characteristic: description
characteristics.text: embryo at different stages of germination and shoots of germinated seeds under chilling stress (13oC/10oC)
characteristics.characteristic: dev-stage
characteristics.text: SolR
characteristics.characteristic: lab-host
characteristics.text: seedlings
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: organ
characteristics.text: Oryza sativa Japonica Group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39947
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: coleotile, radicle, prophyll, immature leaf
characteristics.characteristic: tissue-type
characteristics.text: Rice cold stress germination cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-Zap XR excised as pBluescript
characteristics.characteristic: vector