characteristics.text: LIBEST_001725
characteristics.characteristic: External Id
characteristics.text: SAMN00156297
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2000-03-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:48.783Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Templates for the differential-display RT-PCR were reverse-transcribed from total cellular RNA extracted (1) from excised feeding sites of roots with parasitic nematodes attached to it and (2) from adjacent root pieces of the same size supplemented with non-parasitic nematodes. Expression profiles were compared between (1) and (2) and cDNAs displaying differential expression were cloned into the pCR2.1-TOPO TA-vector (Invitrogen).
characteristics.characteristic: description
characteristics.text: In-vitro cultures of vegetative plants on media plates
characteristics.characteristic: dev-stage
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Ecotype Columbia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005147
characteristics.characteristic: strain
characteristics.text: Excised pieces of roots containing nematode feeding sites
characteristics.characteristic: tissue-type
characteristics.text: Cloned cDNAs of Arabidopsis thaliana generated by mRNA differential-display RT-PCR
characteristics.characteristic: title