characteristics.text: SAMN00154359
characteristics.characteristic: External Id
characteristics.text: LIBEST_020349
characteristics.characteristic: External Id
characteristics.text: Institute of Microbiology, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2006-09-05T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:46.493Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Shandong ecotype
characteristics.characteristic: cultivar
characteristics.text: Total RNA was prepared from the whole Thellungiella plants using Trizol reagent (Gibico, USA). Total RNAs of different time points were mixed and used for further mRNA isolation according to manufacturer's protocol (Qiagen, Germany). Briefly, both first-strand cDNAs were synthesized and digested with Not I. Sal I adaptors were ligated to these digested cDNAs fragments, and then cDNAs of Thellungiella were directionally inserted into the pREP-5N vector.
characteristics.characteristic: description
characteristics.text: 5-week-old seedlings
characteristics.characteristic: dev-stage
characteristics.text: fission yeast
characteristics.characteristic: lab-host
characteristics.text: aerial part tissue
characteristics.characteristic: organ
characteristics.text: Eutrema halophilum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_98038
characteristics.characteristic: organism
characteristics.text: SalI
characteristics.characteristic: re_1
characteristics.text: NotI
characteristics.characteristic: re_2
characteristics.text: Thellungiella halophila fission yeast expression library
characteristics.characteristic: title
characteristics.text: pREP-5N
characteristics.characteristic: vector