characteristics.text: SAMN00156212
characteristics.characteristic: External Id
characteristics.text: LIBEST_001638
characteristics.characteristic: External Id
characteristics.text: University Hospital Nijmegen
characteristics.characteristic: INSDC center name
characteristics.text: 1999-06-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:46.350Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Subtraction of retina cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).
characteristics.characteristic: description
characteristics.text: TOP10F'
characteristics.characteristic: lab-host
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: retina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000966
characteristics.characteristic: tissue-type
characteristics.text: subtracted retina cDNA library
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCRII
characteristics.characteristic: vector