characteristics.text: LIBEST_021506
characteristics.characteristic: External Id
characteristics.text: SAMN00153225
characteristics.characteristic: External Id
characteristics.text: The J. Craig Venter Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2007-07-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:19.790Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Williams 82
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007023
characteristics.characteristic: cultivar
characteristics.text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNAs provided by Steve Clough, Peter Lafayette, Babu Valliyodan, and Madan Bhattacharyya. This library is also called GLMA.
characteristics.characteristic: description
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: drought stressed and control stem, tissue culture suspension, salt stressed, drought stressed, and Pseudomonas infected leaves, etiolated seedlings
characteristics.characteristic: tissue-type
characteristics.text: JCVI-SOY2
characteristics.characteristic: title
characteristics.text: pDNR-LIB (Clontech)
characteristics.characteristic: vector