characteristics.text: LIBEST_001305
characteristics.characteristic: External Id
characteristics.text: SAMN00155889
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 1998-05-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:37.203Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: B73
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007022
characteristics.characteristic: cultivar
characteristics.text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer. The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with XhoI and size-selected. The resulting molecules were directionally cloned into the EcoRI and XhoI sites of the pET30a plasmid (Novagen).
characteristics.characteristic: description
characteristics.text: two-week-old green seedling
characteristics.characteristic: dev-stage
characteristics.text: BL21(DE3)
characteristics.characteristic: lab-host
characteristics.text: green seedlings
characteristics.characteristic: organ
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: above ground tissues
characteristics.characteristic: tissue-type
characteristics.text: ISUM1
characteristics.characteristic: title
characteristics.text: plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pET30a
characteristics.characteristic: vector