characteristics.text: LIBEST_021947
characteristics.characteristic: External Id
characteristics.text: SAMN00153552
characteristics.characteristic: External Id
characteristics.text: Malaysia Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-03-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:31.557Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Total RNA was extracted from Lates calcarifer brain tissue by the guanidium thiocyanate method. Following preparation of Lates calcarifer brain mRNA using the mRNA Purification Kit (Amersham Pharmacia Biotech), cDNA synthesis and library construction were carried out using the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Lates calcarifer brain cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once to produce a library with a titre of 1.16 X 105 pfu. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.
characteristics.characteristic: description
characteristics.text: Juvenile
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000112
characteristics.characteristic: dev-stage
characteristics.text: Brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Lates calcarifer
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8187
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: LBE
characteristics.characteristic: title
characteristics.text: Phagemid
characteristics.characteristic: v_type
characteristics.text: Lambda Uni-ZAP XR
characteristics.characteristic: vector