characteristics.text: LIBEST_023811
characteristics.characteristic: External Id
characteristics.text: SAMN00166769
characteristics.characteristic: External Id
characteristics.text: The J. Craig Venter Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2008-10-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:46:28.163Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A17
characteristics.characteristic: cultivar
characteristics.text: Tissues: drought-stressed leaves, roots and nodules (8 weeks post-inoculation with S. meliloti 2011); fungal-infected roots (3, 5, 7 days post-inoculation with P. omnivora of 4-week-old plant); salt-treated leaves and roots (1, 6 hours post-treatment with 100mM NaCl); cell culture (root-derived cell suspension with equal parts of unelicited and yeast-elicited cell cultures at 3 hrs post-elicitation), and untreated aerial tissues including cotyledons, terminal buds, leaves, petioles and developing pods. Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). RNA provided by Esther Gonzalez, Rao Uppalapati, Sue Miller, Carrol Vance, Florian Frugier, Vagner Benedito and Greg May.
characteristics.characteristic: description
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: SfiIA
characteristics.characteristic: re_1
characteristics.text: SfiIB
characteristics.characteristic: re_2
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: tissue-type
characteristics.text: JCVI-MT2
characteristics.characteristic: title
characteristics.text: pDNR-LIB (Clontech)
characteristics.characteristic: vector