characteristics.text: LIBEST_021324
characteristics.characteristic: External Id
characteristics.text: SAMN00154428
characteristics.characteristic: External Id
characteristics.text: USDA/ARS, Crop Protection and Management Research Unit
characteristics.characteristic: INSDC center name
characteristics.text: 2007-06-20T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:45:15.497Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Tifrunner
characteristics.characteristic: cultivar
characteristics.text: cDNA library was constructed from peanut cultivar Tifrunner (TF) or GT-C20 (C20). Tifrunner is resistant to TSMV and susceptible to Aspergillus parasiticus. GT-C20 is susceptible to TSMV, and has reduced aflatoxin contamination. Developing seeds were challenged by drought stress and Aspergillus, and collected at reproduction stages, R5, R6, or R7. Total RNA was isolated with RNeasy Kit (QIAGEN). mRNA was extracted from total RNA using Oligotex (QIAGEN). cDNA synthesis and library construction followed the protocol of ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Stratagene). The cDNA above 300 bp was collected after size-fraction. The inserts were directionally cloned into ZAP Express vector using XhoI and EcoRI adapters. The lambda library was packed into phage using Gigapack III Gold (Stratagene). The unamplified library was used to excise pBluescript phagemids from the ZAP Express vector, and the phagemids were used to transform the host bacteria XL1-Blue MRF cells.
characteristics.characteristic: description
characteristics.text: R6
characteristics.characteristic: dev-stage
characteristics.text: XL1-Blue MRF
characteristics.characteristic: lab-host
characteristics.text: Developing Seeds
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0004506
characteristics.characteristic: organ
characteristics.text: Arachis hypogaea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3818
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: XhoI
characteristics.characteristic: re_2
characteristics.text: Seed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000991
characteristics.characteristic: tissue-type
characteristics.text: USDA-Tifton Peanut Library TFR6
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScriptIISK+
characteristics.characteristic: vector