characteristics.text: SAMN00153998
characteristics.characteristic: External Id
characteristics.text: LIBEST_013939
characteristics.characteristic: External Id
characteristics.text: National Cancer Institute / NIH
characteristics.characteristic: INSDC center name
characteristics.text: 2003-07-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:41:41.833Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.71 kb (range 0.5-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA) Corp.
characteristics.characteristic: description
characteristics.text: DH10B (phage-resistant)
characteristics.characteristic: lab-host
characteristics.text: Pooled - Molar
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: SfiI (ggccattatggcc)
characteristics.characteristic: re_1
characteristics.text: SfiI (ggccgcctcggcc)
characteristics.characteristic: re_2
characteristics.text: NIH_MGC_190
characteristics.characteristic: title
characteristics.text: plasmid (chloramphenicol resistant, grown at 30C)
characteristics.characteristic: v_type
characteristics.text: pDNR-LIB
characteristics.characteristic: vector