characteristics.text: LIBEST_019836
characteristics.characteristic: External Id
characteristics.text: SAMN00150451
characteristics.characteristic: External Id
characteristics.text: Universita' degli Studi di Torino
characteristics.characteristic: INSDC center name
characteristics.text: 2006-12-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:28.480Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: A cDNAs library was constructed (using the Clontech PCR-Select(TM) cDNA Subtraction Kit (BD Biosciences) following the manifacture's instruction) subtracting cDNA from Medicago truncatula GFP:HDEL-transformed roots pieces contacted by G. margarita appressoria (tester) with cDNA from the corresponding control roots (driver). Before performing the subtraction the cDNAs from the two RNA populations were PCR amplified using the BD SMART PCR cDNA Synthesis Kit (BD Biosciences) using a limited number of cycles to keep the amplification in the exponential phase.
characteristics.characteristic: description
characteristics.text: Medicago truncatula
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3880
characteristics.characteristic: organism
characteristics.text: roots
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: tissue-type
characteristics.text: Medicago truncatula Clontech PCR select cDNA subtraction
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pGEM-T
characteristics.characteristic: vector