characteristics.text: LIBEST_001188
characteristics.characteristic: External Id
characteristics.text: SAMN00155774
characteristics.characteristic: External Id
characteristics.text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
characteristics.characteristic: INSDC center name
characteristics.text: 1998-05-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:35:34.423Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCAAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Wolfgang Liedtke. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
characteristics.characteristic: description
characteristics.text: DH10B
characteristics.characteristic: lab-host
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organ
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Not I
characteristics.characteristic: re_1
characteristics.text: Eco RI
characteristics.characteristic: re_2
characteristics.text: hypothalamus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001898
characteristics.characteristic: tissue-type
characteristics.text: Soares mouse hypothalamus NMHy
characteristics.characteristic: title
characteristics.text: phagemid (ampicillin resistant)
characteristics.characteristic: v_type
characteristics.text: pT7T3D-PacI
characteristics.characteristic: vector