characteristics.text: SAMN00164173
characteristics.characteristic: External Id
characteristics.text: LIBEST_009664
characteristics.characteristic: External Id
characteristics.text: German Diabetes Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2002-01-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:39:43.730Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: J774 A.1 (German Collection of Microorganism and Cell Culture, Braunschweig, Germany)
characteristics.characteristic: cell-line
characteristics.text: Total RNA was isolated by Trizol reagent; poly(A)+RNA was purified using oligotex purification system (Qiagen). After double cDNA synthesis Subtractive Suppression Hybridization was performed using PCR-Select cDNA subtraction kit (Clontech). The subtracted cDNA library was cloned into pCRII.1 vector system (Invitrogen). The differentially expressed messages were evaluated by southern blot as first screening step and then confirmated by northern blot.
characteristics.characteristic: description
characteristics.text: Mouse recombinant IL-10 stimulated macrophages (16 h)
characteristics.characteristic: dev-stage
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Differentially expressed library from murine macrophage after IL-10 stimuli
characteristics.characteristic: title
characteristics.text: pCRII.1 vector system (Invitrogen)
characteristics.characteristic: vector