characteristics.text: SAMN00150288
characteristics.characteristic: External Id
characteristics.text: LIBEST_019672
characteristics.characteristic: External Id
characteristics.text: The University of Georgia, Department of Plant Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2006-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:44:24.660Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: BTx623
characteristics.characteristic: cultivar
characteristics.text: Four-week-old sorghum seedlings were sprayed with spore suspension prepared from 3-week-old FRM42I, a sorghum isolate of the anthracnose pathogen Colletotrichum graminicola. Inoculated plants were kept in a 25 C dark growth chamber with 100% relative humidity for 24 hr, followed by 12/12 hr of light/dark cycle at 25 C with 90% relative humidity for another 24 hr. All leaves were harvested and quick frozen with liquid nitrogen and stored in a -80 C freezer. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.
characteristics.characteristic: description
characteristics.text: SOLR strain of E. coli
characteristics.characteristic: lab-host
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: XhoI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Leaves
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: tissue-type
characteristics.text: Pathogen-induced: compatible
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pBlueScriptSK-
characteristics.characteristic: vector