characteristics.text: SAMN00153925
characteristics.characteristic: External Id
characteristics.text: LIBEST_012360
characteristics.characteristic: External Id
characteristics.text: University of California, Los Angeles
characteristics.characteristic: INSDC center name
characteristics.text: 2002-12-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2017-10-11T17:40:56.147Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Hammond's Dwarf Scarlet
characteristics.characteristic: cultivar
characteristics.text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from suspensor mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The suspensor cDNA fragments were ligated non-directionally into the pCR2.1 vector {Invitrogen), and the recombinant cDNAs were transformed into E. coli INV alpha F' cells (Invitrogen).
characteristics.characteristic: description
characteristics.text: 6-days post-pollination
characteristics.characteristic: dev-stage
characteristics.text: Suspensor Region of Globular-Stage Embryos
characteristics.characteristic: organ
characteristics.text: Phaseolus coccineus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3886
characteristics.characteristic: organism
characteristics.text: EcoRI
characteristics.characteristic: re_1
characteristics.text: EcoRI
characteristics.characteristic: re_2
characteristics.text: Scarlet Runner Bean Suspensor Region pCR2.1
characteristics.characteristic: title
characteristics.text: Plasmid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/SO_0000155
characteristics.characteristic: v_type
characteristics.text: pCR2.1
characteristics.characteristic: vector