SAMD00022972
External Id
The University of Tokyo Graduate School of Frontier Sciences Department of Medical Genome Sciences Laboratory of Functional Genomics
INSDC center name
2015-03-12T08:58:09Z
INSDC first public
2015-03-11T19:58:09Z
INSDC last update
DRS019265
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS019265
SRA accession
LC2/ad
http://www.ebi.ac.uk/efo/EFO_0003140
cell line
The LC2/ad lines were acquired from the RIKEN Bio Resource Center under the catalogue numbers of v. The LC2/ad cell lines were cultured in collagen Type I-coated dishes. 10x6 cells were harvested and used for the single-cell RNA-Seq analyses using the C1 system (Fluidigm). Briefly, 96 cells were captured in the flow cells and separated into independent chambers. First-strand cDNA was synthesized and further amplified using the SMARTer system (clontech). Illumina sequencing libraries were constructed using Nextera XT DNA Sample Preparation kit (illumina). After evaluation of the quality and quantity of the constructed RNA-Seq libraries using a BioAnalyzer (Agilent), sequencing was performed on the HiSeq2500 platform with a 100base paired-end read.
description
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
LC2ad_SingleCell_RNAseq
sample name
LC2ad
strain
LC2ad_SingleCell_RNAseq
title