SAMN02214201
External Id
LIBEST_028237
External Id
Indian Agricultural Research Institute
INSDC center name
2013-06-27T23:05:04.113Z
INSDC first public
2015-05-20T15:03:24.293Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Pusa 16
cultivar
Total RNA was isolated from developing seeds of G.max L.. A PCR based cDNA library was constructed using oligo (dT) primer. The amplified product was cloned in pCR2.1-TOPO vector and transformed in E.coli. The transformants were screened using blue white selection and positive transformants selected by restriction digestion. The recombinants were sequenced using M13 reverse promoter.
description
8-10 mm
dev-stage
DH10B
lab-host
Glycine max
http://purl.obolibrary.org/obo/NCBITaxon_3847
organism
EcoRI
re_1
EcoRI
re_2
seeds
http://www.ebi.ac.uk/efo/EFO_0000991
tissue-type
Glycine max (soybean) late seed development stage library
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pCR2.1-TOPO
vector