SAMN02230665
External Id
LIBGSS_039134
External Id
2017-10-11T14:29:06.987Z
INSDC first public
2017-10-11T14:29:06.987Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
These are the DNA sequences flanking the insertion sites of two sets of P-element transposon insertion mutants of Drosophila melanogaster generated in the lab of William Chia (Morin et al. (2001) Proc. Nat. Acad. Sci. 98: 15050-55). The mutagenesis screen used a mixture of three synthetic transposon vectors: pPGA (FlyBase symbol: P{PTT-GA}, FlyBase ID: Fbtp0017514), pPGB (FlyBase symbol: P{PTT-GB}, FlyBase ID: Fbtp0017513), and pPGC (FlyBase symbol: P{PTT-GC}, FlyBase ID: Fbtp0017512). Samples of frozen flies were prepared in the lab of Lynn Cooley (Yale University). The flanking sequences were amplified by inverse PCR and sequenced at Lawrence Berkeley National Laboratory as described by Quinones-Coello et al. 2007.
description
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
P-element insertion flanks from G and ZCL lines sequenced at LBNL
title