LIBGSS_039053
External Id
SAMN02142114
External Id
Lawrence Berkeley National Laboratory
INSDC center name
2013-05-12T04:05:07.240Z
INSDC first public
2015-05-20T14:13:58.697Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
These are the DNA sequences flanking the insertion sites of a set of P-element transposon insertion mutants of Drosophila melanogaster that are part of the Carnegie protein trap library. The transposon vector is the synthetic construct pPGC (Morin et al. (2001) Proc. Nat. Acad. Sci. 98: 15050-55; FlyBase symbol: P{PTT-GC}, FlyBase ID: Fbtp0017512). Sequences flanking the insertion sites of mutants were amplified by inverse PCR and sequenced at the Lawrence Berkeley National Laboratory using an unpublished modification of the protocol of Bellen et al. 2004 (Genetics 167: 761-781; FlyBase ID: FBrf0179132). A detailed protocol may be downloaded from http://flypush.imgen.bcm.tmc.edu/pscreen/
description
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
CC P-element insertion flanking sequences
title