SAMN01999538
External Id
Karolinska Institutet
INSDC center name
2014-10-01T00:00:00Z
INSDC first public
2021-02-28T02:05:18.281Z
INSDC last update
SRS415759
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS415759
SRA accession
Mixed sample mother and kid
age
Blood
http://purl.obolibrary.org/obo/UBERON_0000178
body site
2010-11-10
collection date
The mixed sample 95:5 was mixed with 95% healthy female DNA and 5% Down syndrome DNA. The sample was diluted to around 20 million molecules. This means that each molecule has a unique starting position in the genome, i.e. it is unique with a high probability. The mixed library was then amplified and sequenced until saturation that is each molecules is seen preferably at least two times. It is then possible to bioinformatically remove molecule copies. The idea is that all or most molecules before amplification should be seen after sequencing, and the bias from amplification should be removed.
description
Sweden
geo loc name
skip
http://www.ebi.ac.uk/efo/EFO_0010129
is reference
Karoliska Hospital
isolation source
Mixed Samples
label
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Mixed healthy and Down syndrome. 95% healthy DNA and 5% Down syndrome DNA
phenotype
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
specific host
Mixed Sample 95:5
title