LIBEST_028220
External Id
SAMN02191665
External Id
Embrapa Genetic Resources and Biotechnology
INSDC center name
2013-07-31T07:44:06.290Z
INSDC first public
2015-05-20T15:03:21.560Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
A subtractive cDNA library of Arachis magna leaf tissue was constructed using PCR-Select cDNA Subtraction Kit (Clontech, Mountain View, USA). Subtractive hybridizations were performed using cDNA from stressed plant leaves (tester) against cDNA from well watered plant leaves (driver). The differential products obtained were cloned into pGEM T-Easy (Promega) and sequenced in ABI Prism 3700 DNA Analyser (Applied Biosystems Inc.). A minimum insert size of 30 bp and at least 20 pb with quality of phred > 20 were considered for the analysis.
description
E. coli DH5alpha
lab-host
leaves
http://purl.obolibrary.org/obo/PO_0025034
organ
Arachis magna
http://purl.obolibrary.org/obo/NCBITaxon_108213
organism
EcoRI
re_1
SpeI
re_2
KG30097
strain
AM1 Arachis magna drought stressed SSH library
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEM-T Easy Vector
vector