Zero Time-R1
ACACAGTGAGT
Day-5-12C-CH4+Urea-Fraction-13
ACACGACGACT
Zero Time-R2, Day-5-12C-CH4+Urea-Fraction-12
ACACGTAGTAT
Zero Time-R3, Day-5-12C-CH4+Urea-Fraction-11
ACACTACTCGT
Day-5-13C-CH4+Urea-Fraction-8
ACACTCATACT
Day-5-13C-CH4+Urea-Fraction-7
ACAGACAGCGT
Day-5-13C-CH4+Urea-Fraction-6
ACAGACTATAT
Day-5-13C-CH4+Urea-Fraction-5
ACAGAGACTCT
Day-5-13C-CH4+Urea-Fraction-4
ACAGCTCGTGT
Day-5-13C-CH4-Fraction-13
ACAGTGTCGAT
Day-5-12C-CH4+Urea-Fraction-10
ACGACACGTAT
Day-5-13C-CH4-Fraction-12
ACGAGCGCGCT
Day-5-12C-CH4+Urea-Fraction-9
ACGAGTAGACT
Day-5-13C-CH4-Fraction-11
ACGATGAGTGT
Day-5-13C-CH4-Fraction-10
ACGCGAGAGAT
Day-5-12C-CH4+Urea-Fraction-8
ACGCGTCTAGT
Day-5-13C-CH4-Fraction-9
ACGCTCTCTCT
Day-5-12C-CH4+Urea-Fraction-7
ACGTACACACT
Day-5-12C-CH4+Urea-Fraction-6
ACGTACTGTGT
Day-5-12C-CH4+Urea-Fraction-5
ACGTAGATCGT
Day-5-13C-CH4-Fraction-8
ACGTCGCTGAT
Day-5-13C-CH4-Fraction-7
ACGTCTAGCAT
Day-5-12C-CH4+Urea-Fraction-4
ACTACGTCTCT
Day-5-13C-CH4-Fraction-6
ACTAGTGATAT
Day-5-13C-CH4+Urea-Fraction-13
ACTATACGAGT
Day-5-13C-CH4-Fraction-5
ACTCACACTGT
Day-5-13C-CH4-Fraction-4
ACTCACTAGCT
Day-5-13C-CH4+Urea-Fraction-12
ACTCGCGTCGT
Day-5-13C-Urea-Fraction-13
ACTCTATATAT
Day-5-13C-Urea-Fraction-12
ACTGATCTCGT
Day-5-13C-Urea-Fraction-11
ACTGCTGTACT
Day-5-13C-Urea-Fraction-10
ACTGTAGCGCT
Day-5-13C-Urea-Fraction-9
AGACACTCACT
Day-5-13C-Urea-Fraction-8
AGACATATAGT
Day-5-13C-Urea-Fraction-7
AGACGTGATCT
Day-5-13C-CH4+Urea-Fraction-11
AGACTCGACGT
Day-5-13C-Urea-Fraction-6
AGAGTACAGAT
Day-5-13C-Urea-Fraction-5
AGAGTATCTCT
Day-5-13C-Urea-Fraction-4
AGATACGCTGT
Day-19-12C-CH4+Urea-Fraction-13
AGATCTAGTCT
Day-19-12C-CH4+Urea-Fraction-12
AGCAGCGTAGT
Day-19-12C-CH4+Urea-Fraction-11
AGCGCACGAGT
Day-19-12C-CH4+Urea-Fraction-10
AGCGTGTGCGT
Day-19-12C-CH4+Urea-Fraction-9
AGCTAGATACT
Day-19-12C-CH4+Urea-Fraction-8
AGCTGTCGACT
Day-5-13C-CH4+Urea-Fraction-10
AGTACGAGAGT
Day-5-13C-CH4+Urea-Fraction-9
AGTACTACTAT
Day19-12C-CH4+Urea-R1
AGTAGACGTCT
Day-19-12C-CH4+Urea-Fraction-7
AGTATGCACGT
Day-19-12C-CH4+Urea-Fraction-6
AGTCGCGCTAT
Day-19-12C-CH4+Urea-R2
AGTCGTACACT
Day-19-12C-CH4+Urea-Fraction-5
AGTCTGTCTGT
Day-19-12C-CH4+Urea-R3
AGTGTAGTAGT
Day-19-12C-CH4+Urea-Fraction-4
ATACACACGAT
Day-19-13C-CH4+Urea-Fraction-13
ATACGCGTGCT
Day-19-13C-CH4+Urea-Fraction-12
ATACTAGCACT
Day-19-13C-CH4+Urea-Fraction-11
ATAGAGCTAGT
Day19-13C-CH4+Urea-R1
ATAGTATACGT
Day5-12C-CH4+Urea-R1, Day-19-13C-CH4+Urea-Fraction-10
ATATAGAGTAT
Day5-12C-CH4+Urea-R2, Day-19-13C-CH4+Urea-Fraction-9
ATCGCTCACGT
Day5-12C-CH4+Urea-R3, Day-19-13C-CH4+Urea-Fraction-8
ATCGTCAGTCT
Day5-13C-CH4+Urea-R1, Day-19-13C-CH4+Urea-Fraction-7
ATCTCTCGTAT
Day5-13C-CH4+Urea-R2, Day-19-13C-CH4+Urea-Fraction-6
ATCTGAGACGT
Day5-13C-CH4+Urea-R3, Day-19-13C-CH4+Urea-Fraction-5
ATGCTACGTCT
Day5-13C-CH4-R1, Day-19-13C-CH4+Urea-Fraction-4
ATGTGACTACT
Day5-13C-CH4-R2, Day-19-13C-CH4-Fraction-13
CACGAGACAGT
Day5-13C-CH4-R3, Day-19-13C-CH4-Fraction-12
CACGCGAGTCT
Day5-13C-Urea-R1, Day-19-13C-CH4-Fraction-11
CACGCTACGAT
Day5-13C-Urea-R2, Day-19-13C-CH4-Fraction-10
CACTACGATGT
Day5-13C-Urea-R3
CACTATACTCT
Day-19-13C-CH4+Urea-R2
CAGTACGTACT
Day-19-13C-CH4-Fraction-9
CATAGTCGCGT
Day-19-13C-CH4+Urea-R3
CGACGACGCGT
Day19-13C-CH4-R1
CGACGAGTACT
Day-19-13C-CH4-Fraction-8
CGAGACACTAT
Day-19-13C-CH4-Fraction-7
CGAGAGTGTGT
Day-19-13C-CH4-Fraction-6
CGAGTCATCGT
Day-19-13C-CH4-R2
CGATACTACGT
Day-19-13C-CH4-Fraction-5
CGATCGTATAT
Day-19-13C-CH4-Fraction-4
CGCAGTACGCT
Day-19-13C-Urea-Fraction-12
CGCGCTATACT
Day-19-13C-Urea-Fraction-11
CGTACAGATAT
Day-19-13C-CH4-R3
CGTACGTCGAT
Day-19-13C-Urea-Fraction-10
CGTAGCTCTCT
Day-19-13C-Urea-Fraction-9
CGTATAGTGCT
Day-19-13C-Urea-Fraction-8
CGTCAGCGACT
Day-19-13C-Urea-Fraction-7
CGTCGCAGTGT
Day-19-13C-Urea-Fraction-6
CGTCTCACGAT
Day-19-13C-Urea-Fraction-5
CGTGACTCAGT
Day-19-13C-Urea-Fraction-4
CTACACGCTCT
Day-19-13C-Urea-Fraction-13
CTACGATATGT
Day19-13C-Urea-R1
CTACTCGTAGT
Day-19-12C-CH4+Urea-Fraction-3
CTAGACAGACT
Day-19-13C-CH4+Urea-Fraction-3
CTAGTACTCAT
Day-19-13C-CH4-Fraction-3
CTATATGTCGT
Day-19-13C-Urea-Fraction-3
CTATCGACACT
SAMD00013029
External Id
Day-19-13C-Urea-R2
GTACAGTACGT
Day-19-13C-Urea-R3
GTCGTACGTAT
Soil Environment and Pollution Remediation Laboratory, Institute of Soil Science, Chinese Academy of Sciences
INSDC center name
2013-12-19T00:00:00Z
INSDC first public
2014-11-12T03:29:31Z
INSDC last update
DRS013070
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS013070
SRA accession
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
The high-throughput sequencing of 16S rRNA genes at the whole community level provides an almost unbiased profiling strategy for measuring characteristic changes in the relative prportions of the microcosms involved in certain functional process in complex systems, such as soil. There are 27 samples for total DNA pyrosequencing and 84 samples for SIP fractionated DNA. The paddy soil cllected from Yangzhou city of Jiangsu province was used for study. Four treatments were performed including 13C-CH4-labeled microcosms (incubated with 13C-CH4), 13C-Urea-labeled microcosms (incubated with 13C-Urea and 13C-CO2), 13C-CH4+Urea-labeled microcosms (incubated with 13C-CH4, 13C-Urea and 13C-CO2) and 12C-CH4+Urea control microcosm (incubated with 12C-CH4, 12C-Urea and 12C-CO2). The 13C-labeled urea can hydrolyze to ammonia and 13C-CO2, which is benificial for the 13C-label of autotrophic ammonia oxidizer. Pairwise comparison among the treatments of 13C-CH4, 13C-CH4+Urea, and 13C-Urea was used to assess the effect of urea fertilization on methane oxidation, and the role of methane on ammonia oxidation. The soil microcosm with 12C-CH4+Urea amendment was performed as control treatment for the labeled SIP microcosms.
sample comment
DRS013070
sample name
Competitive interactions between methane- and ammonia oxidizing bacteria modulate carbon and nitrogen cycling in paddy soil
title