SAMN02028956
External Id
Biocant
INSDC center name
2013-09-23T07:44:08.800Z
INSDC first public
2014-06-03T16:22:30.590Z
INSDC last update
SRS410826
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS410826
SRA accession
B. xylophilus from Portugal, from Santa Comba Dão (1 isolate), was grown in fungal culture (Botrytis cinerea. The total RNA was isolated from mixed stage cultures with the Trizol method. cDNA was synthesized with MINT cDNA synthesis kit (Evrogen, Moscow, Russia) to amplify selectively mRNA through polyA tails, and normalized according to the Duplex-Specific Nuclease-technology, following the instructions of the TRIMMER cDNA Normalization kit (Evrogen, Moscow, Russia). 454 pyrosequencing of each normalized library was performed according to 454 standard procedures.
description
Bursaphelenchus xylophilus
http://purl.obolibrary.org/obo/NCBITaxon_6326
organism
B. xylophilus isolate from Portugal, from Santa Comba Dão
title