SAMN01942539
External Id
Vanderbilt University
INSDC center name
2013-09-17T06:49:07.767Z
INSDC first public
2017-02-10T11:05:07.373Z
INSDC last update
SRS399738
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS399738
SRA accession
Human cell lines were grown exponentially at 37C at 5% CO2 in DMEM supplemented with 10% bovine calf serum, then subjected to 1% formaldehyde crosslinking for 10 min at 25C. Cells were harvested, lysed, and the purified chromatin was sonicated by standard methods. With the following modifications, ChIP-exo was carried out as previously described (Rhee and Pugh, 2012) with chromatin extracted from 10 million cells (K562, ATCC), ProteinG MagSepharose resin (GE Healthcare), and 3 ug of Pol II (Santa Cruz Biotech, sc-899, directed against the N-terminus of the Pol II large subunit encoded by POL2RA).
description
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
R29L7I34 human GTFs ChIP-exo
title