SAMN02342649
External Id
NIAMS/NIH
INSDC center name
2013-12-26T06:46:31.873Z
INSDC first public
2014-06-09T18:20:43.977Z
INSDC last update
SRS478091
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS478091
SRA accession
6-8 weeks
http://purl.obolibrary.org/obo/UO_0000034
age
C57BL/6
http://www.ebi.ac.uk/efo/EFO_0004472
breed
splenic B cells
http://purl.obolibrary.org/obo/CL_0000785
cell type
Resting splenic B cells were isolated from 6-8 week-old wild-type C57BL6/J mice with anti-CD43 Microbeads (anti-Ly48; Miltenyi Biotech) and were activated for 48-60 h with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml; Sigma) and 0.5 μg ml−1 of anti-CD180 (RP105) antibody (RP/14, BD Pharmingen).
description
Resting splenic B cells were isolated from 6-8 week-old wild-type C57BL6/J mice with anti-CD43 Microbeads (anti-Ly48; Miltenyi Biotech) and were activated for 48-60 h with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml; Sigma) and 0.5 μg ml−1 of anti-CD180 (RP105) antibody (RP/14, BD Pharmingen).
description
wild-type
http://www.ebi.ac.uk/efo/EFO_0005168
genotype
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
wild-type activated B cells (Mus musculus)
title