SAMD00009659
External Id
Subsurface Geobiology and Advanced Research Project, Extremobiosphere Research Program, Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology
INSDC center name
2016-09-16T00:00:00Z
INSDC first public
2016-09-16T09:00:12Z
INSDC last update
DRS001479
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS001479
SRA accession
uncultured marine microorganism
http://purl.obolibrary.org/obo/NCBITaxon_415540
organism
White Head chimney was an active clear smoker chimney and was found at Edmond field (23Â°52.6772'S, 69Â°35.7847'E). The top of the chimney was covered with white sulfur particle. The vent fluid temperature was 264Â°C. We sampled the chimney and divided into the surface and inside layer. We extracted DNA from each layer, and subjected to PCR amplification. But no fragment was amplified. Therefore, we analyzed only DNA fragments from surface l layer of the chimney. The amplified 200 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.
sample comment
DRS001479
sample name
16S rRNA gene fragments from surface layer of White Head chimney in Edmond field.
title