SAMD00004152
External Id
Laboratory of Bio-resource Regulation, Department of Integrated Biosciences
INSDC center name
2013-06-25T00:00:00Z
INSDC first public
2014-11-12T03:28:52Z
INSDC last update
DRS011874
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS011874
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
ZHBTc4 is derived from ZHTc6 and maintained by tetracycline (Tc)-regulatable Oct-3/4 transgene. Both of Oct-3/4 locus are disrupted by IRES-zeocin and IRES-BSD KO vectors. It should be cultured in the absence of tetraycline to maintain the undifferentiated state. pCAGGS vector was used to generate the transgenic ES cell lines constitutively expressing Flag-H3.3 variant. A DNA fragment coding the histone variant tagged with Flag on its N-terminal was inserted into EcoRI site of the pCAGGS vector. Puromycin N-acetyl-transferase (Pac) gene obtained from salI digestion of pCre-Pac was inserted into the salI site of the vectors. The vector coding a Flag-histone variant was transfected into the ZHBTc4 cells by lipofection, and the cells were selected in the ES medium with 100 ÃÂ¼g/mL of puromycin. A single colony of the cells thus obtained for the variant was picked up and cultured.
sample comment
DRS011874
sample name
ZHBTc4 mouse ES cells stably expressing Flag-H3.3 variant
title