SAMD00010730
External Id
Department of Life Science and Informatics, Maebashi Institute of Technology
INSDC center name
2013-04-01T00:00:00Z
INSDC first public
2014-11-12T03:29:22Z
INSDC last update
DRS002605
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS002605
SRA accession
Bacillus subtilis
http://purl.obolibrary.org/obo/NCBITaxon_1423
organism
Chromatin immunoprecipitation (ChIP) is a common method to map protein-binding sites in vivo. Combining this method with microarray (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows us to detect protein-binding sites scattered across the entire genome. An enduring challenge for these methods, however, is how to increase their positional resolution. Here, we describe a new method, “Genome Footprinting by high-throughput sequencing (GeF-seq)”, to attain high-resolution mapping of protein-binding sites by combining in vivo DNase I digestion and ChIP-seq. We used GeF-seq to determine the binding site of Bacillus subtilis transition state regulator, AbrB, across the genome.
sample comment
DRS002605
sample name
Genome-wide analysis of in vivo AbrB binding sites in B. subtilis using GeF-seq
title