SAMD00000818
External Id
Arima lab., Department of Informative Genetics, Environment and Genome Research Center, Graduate School of Medicine, Tohoku University
INSDC center name
2013-09-11T00:00:00Z
INSDC first public
2014-11-12T03:28:36Z
INSDC last update
DRS001361
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS001361
SRA accession
Oryzias latipes
http://purl.obolibrary.org/obo/NCBITaxon_8090
organism
Total RNA was extracted using RNeasy mini Kit and RNase-free DNase (Qiagen, CA, USA) from a Hd-rR adult fish. Four micrograms of total RNA was used for library construction using TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturer’s protocol. Briefly, poly-A containing mRNAs were purified using poly-T oligo-attached magnetic beads. The purified mRNAs were fragmented using divalent cations under elevated temperatures, and then converted to dsDNA by two rounds of cDNA synthesis using reverse transcriptase and DNA polymerase I. After an end repair process, DNA fragments were ligated with adaptor oligos. The ligated products were amplified using 15 cycles of PCR to generate RNA-seq library. Library integrity was verified by Bioanalyzer DNA1000 assay (Agilent technologies). Sequencing was performed in 39bp paired end mode using Genome Analyzer IIx (Illumina).
sample comment
DRS001361
sample name
Hd-rR
title