SAMN01994466
External Id
LIBEST_028143
External Id
Centro de Investigaciones Biologicas del Noroeste, S.C.
INSDC center name
2013-03-29T23:05:09.593Z
INSDC first public
2015-05-20T15:03:08.813Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Suppression subtractive hybridization (SSH) was performed to identify the differentially expressed genes during spermatogenesis. The secondary PCR product was non-directionally cloned in pGEM-T vector (Promega) and sequenced based on Sanger sequencing method. The obtained reads were submitted to base-calling by PHRED software, and then the removal of vector and adapter sequences was performed using CUTADAPT.
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev-stage
maturing testis
organ
Nodipecten subnodosus
http://purl.obolibrary.org/obo/NCBITaxon_330909
organism
hermaphrodite
http://purl.obolibrary.org/obo/PATO_0001340
sex
SSH library of maturing testis, subtracted with inactive gonad
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEM-T or pCR4.0
vector