SAMN02364255
External Id
DOE-JGI
INSDC center name
2013-09-26T13:43:55.140Z
INSDC first public
2015-11-12T09:00:21.010Z
INSDC last update
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.water
NCBI submission model
MIMS.me.water.5.0
NCBI submission package
Terrestrial Hot Spring
biome
2007
collection date
20 cm
http://purl.obolibrary.org/obo/UO_0000015
depth
High-temperature (> 75 °C) ecosystems in Yellowstone National Park (YNP) provide an unparalleled opportunity to study chemotrophic archaea and their role in microbial community structure and function under highly constrained geochemical conditions. Acidilobus spp. (order Desulfurococcales) comprise one of the dominant phylotypes in hypoxic geothermal sulfur sediment and Fe(III)-oxide environments along with members of the Thermoproteales and Sulfolobales. Consequently, the primary goals of the current study were to analyze and compare replicate de novo sequence assemblies of Acidilobus-like populations from four different mildly-acidic (pH 3.3-6.1) high-temperature environments (72-82 °C), and to identify metabolic pathways and/or protein-encoding genes that provide a detailed foundation of the potential functional role of these populations in situ. De novo assemblies of the highly similar Acidilobus-like populations (>99% nt 16S rRNA) represent near-complete consensus genomes based on an inventory of single-copy genes, deduced metabolic potential, and assembly statistics generated across sites. Functional analysis of coding sequence and confirmation of gene expression by Acidilobus-like populations provide evidence that they are primarily chemoorganoheterotrophs, generating acetyl-CoA via the degradation of carbohydrates, lipids, and proteins and auxotrophic with respect to several external vitamins, cofactors, and metabolites. No obvious pathways or protein-encoding genes responsible for the dissimilatory reduction of sulfur were identified. The presence of a formate dehydrogenase (Fdh) and other protein-encoding genes involved in mixed acid fermentation support the hypothesis that Acidilobus spp. function as degraders of complex organic constituents in high-temperature, mildly-acidic, hypoxic geothermal systems.
description
MIGS/MIMS/MIMARKS.water
env package
TRUE
environmental sample
Hot Spring (Monarch Geyser)
feature
USA: Yellowstone National Park
geo loc name
metagenome
investigation type
Monarch Geyser (MG_3); temperature 80C; pH 4.0
isolation-source
Monarch Geyser Acidilobus sp.
label
44.7243 N 110.7056 W
lat lon
sediment
material
hot springs metagenome
metagenome source
TRUE
metagenomic
hot springs metagenome
http://purl.obolibrary.org/obo/NCBITaxon_433727
organism
Analysis of sequences collected from Monarch Geyser, YNP
project name
Inskeep et al., 2013a
ref_biomaterial
anaerobe
rel_to_oxygen
Environmental/Metagenome sample for uncultured Acidilobus sp. MG
title