LIBEST_028161
External Id
SAMN01999657
External Id
Malaysia Genome Institute
INSDC center name
2013-04-06T23:05:05.500Z
INSDC first public
2015-05-20T15:03:12.290Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
This cDNA library was constructed from cells that were exposed at 15 C. Total RNA from Glaciozyma antarctica was extracted using TRIZOL reagent as described by manufacturer (Invitrogen, USA). Subsequently, mRNA isolation was carried out using the PolyAT-tract mRNA Isolation Systems (Promega, USA) according to the standard manufacturer's instructions. Then mRNA was verified by Bioanalyzer RNA Nanochip (Agilent Technologies). 3ug of mRNA was used to construct the library using the CloneMiner cDNA Library Construction Kit (Invitrogen, USA) according to the manufacturer's protocol with some modifications. Three different cDNA libraries with different growth temperature were constructed.
description
log phase
dev-stage
ElectroMAX DH10B T1 Phage Resistant Cells
lab-host
Glaciozyma antarctica
http://purl.obolibrary.org/obo/NCBITaxon_105987
organism
BsrGI
re_1
PI12
http://purl.obolibrary.org/obo/CHEBI_90524
strain
La01
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pDONR 222
vector