LIBGSS_039060
External Id
SAMN02202234
External Id
Texas A&M University
INSDC center name
2013-11-02T04:05:04.423Z
INSDC first public
2015-05-20T14:13:59.327Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Genomic DNA was subjected to whole genome amplification using GenomePlex Complete Whole Genome Amplification (WGA) Kit according manufacturer's standard protocol (Sigma-Aldrich Co., St. Louis, MO). Amplified gDNA products were fractionated in agarose gels and fractions containing fragments between 0.5 - 2 kb were collected and purified. After an incubation at 72 C for 20 min in a regular PCR reaction buffer to add a single adenine overhang to the 3'-end, the products were ligated into pCR2.1-TOPO vector using a TOPO-TA cloning kit (Invitrogen, Life Technologies, Grand Island, NY).
description
adult worm
dev-stage
Bobwhite quail
lab-host
Oxyspirura petrowi
http://purl.obolibrary.org/obo/NCBITaxon_1344031
organism
QEW WGA library 2013
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pCR2.1-TOPO
vector