SAMD00009820
External Id
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
INSDC center name
2016-04-04T16:50:54Z
INSDC first public
2016-04-04T03:50:54Z
INSDC last update
DRS016176
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS016176
SRA accession
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
This sample is total RNA in the siNEAT1-depleted and poly I:C stimulated Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2. According to the manufacturer’s protocol, cells were transfected with poly I:C using Lipofectamine 2000 (Invitrogen). For poly I:C stimulation, cells were seeded into 12-well cell culture plates followed by transfection using the indicated amounts of poly I:C and Lipofectamine 2000. The siNEAT1 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siNEAT1 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.
sample comment
DRS016176
sample name
siNEAT1_polyC_plus
title