SAMN02196113
External Id
Integrative Genomics and Bioinformatics Core, Salk Institute
INSDC center name
2013-06-11T12:01:01.430Z
INSDC first public
2015-01-07T02:05:09.447Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
pre-pro-B cell
cell type
PU.1 (sc-352)
chip antibody
PU.1 (Vendor: Santa Cruz, cat# sc-352, lot# F1808)
chip antibody
E2A-/-
http://www.ebi.ac.uk/efo/EFO_0009273
genotype/variation
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
E2A-/- pre-pro-B cell
source_name
C57BL/6
http://www.ebi.ac.uk/efo/EFO_0004472
strain
E2AKO-PU.1-bHLH-ER-ChIP-Seq
title
bHLH-ER
transgenes
The cells were spin-infected with a retrovirus expressing truncated E2A (lacking both activation domains) fused to the estrogen receptor ligand binding domain, as well as hCD25 from a second cistron. After 16 h in culture, cells were treated with tamoxifen (1 µM final concentration) for 6 h. Cells expressing hCD25 were then enriched magnetically (Miltenyi).
treatment