SAMD00000739
External Id
Laboratory of Pomology, Faculty of Agriculture, Ehime University
INSDC center name
2013-10-29T00:00:00Z
INSDC first public
2015-10-12T22:02:37Z
INSDC last update
DRS012266
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS012266
SRA accession
Prunus mume
http://purl.obolibrary.org/obo/NCBITaxon_102107
organism
Pistils were sampled from unopened flowers of Japanese apricot 'Nanko' at the balloon stage of development. Forty pistils were placed on 1% (w/v) agar in each petri dish and incubated at 20ÃÂ°C overnight. After the overnight incubation, three different pollination treatments were conducted for the pistils. Two subsets of the pistils were pollinated by 'Nanko' (self-pollination, SP) or 'Kairyo-Uchida-Ume' (cross-pollination, CP). One third of the pistils were kept unpollinated (UP). The SP and CP pistils were sampled at 3, 6, and 9 hours after pollination. The UP pistils were also sampled at the same times as the pollinated pistils. A total of one hundred pistils equivalent to the number incubated in two and half petri dishes were sampled at each time point after pollination and pooled for the SP, CP, and UP treatments. Thus, three hundred pistils were used for RNA extraction for each treatment.
sample comment
DRS012266
sample name
Cross-pollinated pistils of Japanese apricot 'Nanko'
title