SAMN02315627
External Id
University of Cambridge
INSDC center name
2014-08-12T00:00:00Z
INSDC first public
2019-06-20T16:35:58.951Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
6-9
age
Protocols: We crossed virgin females from the Australian line to males of a T(2;3)CyO-TM6/pr cn; mwh ry[506] e balancer stock in which the second and third chromosomes co-segregate. A single male from the progeny exhibiting the balancer phenotype was then crossed with y; cn bw sp virgin females from the reference stock, and offspring not exhibiting the balancer phenotype were collected for sequencing. We homogenized flies in Trizol reagent and extracted RNA using Direct-zol RNA MiniPrep kits according to the manufacturer’s protocol (Zymo Research, Irvine, CA). Illumina TruSeq libraries were constructed. These were sequenced on an Illumina HiSeq2000.
description
Adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev stage
C12
label
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
Drosophila melanogaster C12 (Innisfail)
title