SAMN02190948
External Id
LIBEST_028216
External Id
Latvian State Forest Research Institute Silava
INSDC center name
2013-06-05T23:05:04.527Z
INSDC first public
2015-05-20T15:03:20.990Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Two year old scots pine seedlings were maintained 24 h in gradually increasing temperature to 40 degree Celsius. After 24 h needles was collected and frozen in liquid nitrogen. RNA was isolated using a CTAB-based method followed by several purifications with the Turbo DNA-free kit (Ambion). Reverse transcription was performed with random hexamer primers using 1 mkg of RNA with the TaqMan reverse transcription Kit (Applied Biosystems).Differentially expressed fragments were ligated into pTZ57R/T vector with InsTAclone PCR cloning kit (Thermo Scientific) and then transformed by heat shock into E.coli competent cells. Sequencing was performed by Sanger's method from both sides with M13 primers and BigDye Terminator v3.1 Cycle sequencing Kit (Applied Biosystems). Plasmid sequence was removed.
description
needle
organ
Pinus sylvestris
http://purl.obolibrary.org/obo/NCBITaxon_3349
organism
Differential expression after heat shock
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pTZ57R/T
vector