SAMN01942535
External Id
Vanderbilt University
INSDC center name
2013-09-17T06:49:07.597Z
INSDC first public
2017-02-10T11:05:06.960Z
INSDC last update
SRS399734
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS399734
SRA accession
Human cell lines were grown exponentially at 37C at 5% CO2 in DMEM supplemented with 10% bovine calf serum, then subjected to 1% formaldehyde crosslinking for 10 min at 25C. Cells were harvested, lysed, and the purified chromatin was sonicated by standard methods. With the following modifications, ChIP-exo was carried out as previously described (Rhee and Pugh, 2012) with chromatin extracted from 10 million cells (HepG2, ATCC), ProteinG MagSepharose resin (GE Healthcare), and 3 ug of TFIIB (Santa Cruz Biotech, sc-225).
description
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
R18L6I27-35-43 human GTFs ChIP-exo
title