SAMD00009819
External Id
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
INSDC center name
2016-04-04T16:50:54Z
INSDC first public
2016-04-04T03:50:54Z
INSDC last update
DRS016179
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS016179
SRA accession
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
This sample is total RNA in the siPSF-4-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siPSF-4 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siPSF-4 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.
sample comment
DRS016179
sample name
siPSF-4
title