SAMN02211816
External Id
Chair for Microbial Ecology, Technische Universität München
INSDC center name
2014-07-20T00:00:00Z
INSDC first public
2019-06-20T16:33:19.344Z
INSDC last update
SRS450552
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS450552
SRA accession
Radish sprout seeds were sterilized (5 min 70% ethanol, 10 min 1% NaOCl with 0.1% Tween), then washed five times with sterile water and subsequently incubated in sterile MS medium without glucose in sterile plastic boxes (1 L total volume, passively aerated). After germination, seedlings were tested for sterility by plating a sample on LB agar. After 5 days of growth, the shoots were inoculated 10 min with 1 L ¼ concentrated Ringer solution containing 103 cfu/ml EHEC. The superfluous medium was decanted and cfu/g was periodically determined as follows: infected shoots were washed and bacterial numbers of the washing liquid were determined by serial dilution platings. After 120 hours, the transition from exponential to stationary phase could be determined. Bacteria were harvested by gently shaking the seedlings in cold ¼-concentrated Ringer (+1% Tween-20; 4°C) for 1 min. Bacteria were collected by centrifugation from the decanted Ringer as above.
growth condition and treatment
late logarthmic, early stationary phase
growth phase
Escherichia coli O157:H7 str. EDL933
http://purl.obolibrary.org/obo/NCBITaxon_155864
organism
whole organisms
http://purl.obolibrary.org/obo/UBERON_0000468
source_name
EDL933
strain
radish sprouts biological replicate SOLiD
title