SAMN01924518
External Id
LIBGSS_039019
External Id
King Saud University
INSDC center name
2013-02-24T04:05:04.857Z
INSDC first public
2015-05-20T14:13:56.443Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Sukary
cultivar
SSR enriched library was constructed using genomic DNA isolated from date palm variety Sukary. These enriched fragments were used for TA cloning using pGEM Easy cloning kit (Promega, Madison, WI, USA) following manufacturer's recommended protocol. Transformed bacterial clones were sequenced using ABI 3730xl DNA automatic capillary sequencer and the ABI Big Dye Terminator sequencing kit. Software codonecode aligner (CodonCode Corporation, Centerville, MA, USA) was used for base calling and vector sequence removal. Low quality sequences were also omitted. Sequences obtained were screened for di, tri, tetra and penta simple sequence repeats (SSR) using batch primer3 software.
description
fruiting stage
dev-stage
Phoenix dactylifera
http://purl.obolibrary.org/obo/NCBITaxon_42345
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
Phoenix dactylifera cv. Sukary SSR enriched library
title