SAMN01925601
External Id
INRA
INSDC center name
2013-03-08T00:00:00Z
INSDC first public
2019-09-18T14:42:13.676Z
INSDC last update
SRS399155
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS399155
SRA accession
larval stage
http://purl.obolibrary.org/obo/UBERON_0000069
age
2009
collection date
The aim of this study was to create transcriptome resources for the lepidopter sibling species Ostrinia scapulalis (adzuki bean borer) and O. nubilalis (European corn borer). This BioProject sample entry holds the raw and assembled data of the O. nubilalis transcriptome. O. nubilalis samples were collected on maize in northern France. RNA was extracted from a pool of heads and thorax of four adult moths (two males and two females) and postprocessed. The synthesized cDNA was normalized by one cycle of denaturation and reassociation. After separation of reassociated ds-cDNA the remaining ss-cDNA was amplified with 13 PCR cycles. The normalized cDNA library (range of 500-700bp) was sequenced in one GS FLX Titanium (Roche 454) standard chemistry run (1/4 plate). The raw sequences were cleaned (adapter sequences, species barcodes, ribosomal sequences and too short sequences were removed) and then assembled with Newbler (v. 2.5.3).
description
larval stage
http://purl.obolibrary.org/obo/UBERON_0000069
dev stage
France: northern, Loos en Gohelle
geo loc name
sampled on maize
isolation source
Ostrinia nubilalis
http://purl.obolibrary.org/obo/NCBITaxon_29057
organism
pooled male and female
sex
29057
specific host
European corn borer
strain
head and thorax
tissue type
Ostrinia nubilalis 454 transcriptome
title