SAMD00003852
External Id
Plant Genome Research, Agrogenomics Research Center, National Institute of Agrobiological Sciences
INSDC center name
2013-03-20T00:00:00Z
INSDC first public
2014-11-12T03:28:51Z
INSDC last update
DRS005718
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS005718
SRA accession
Sorghum bicolor
http://purl.obolibrary.org/obo/NCBITaxon_4558
organism
Sorghum (Sorghum bicolor L. Moench) SIL-05, which is resistant to target leaf spot, was used for mRNA seq. Four seeds were sterilized, placed onto Kumiai-Ryujyou-Baido soil (Kureha Chemical, Tokyo, Japan) in each plant box (5Ã5Ã15 cm high) and incubated in a chamber for 7 days under 16 h light and 8 h dark at 28Â°C. On day 7 (about the 2- or 3-leaf stage) the sorghum plants were sprayed with 1 ml of 0.01% Tween-20 solution per plant box. The plants were sampled at 24 h after inoculation. Four biological replicates were collected, immediately frozen in liquid nitrogen, and mixed, to minimize the effect of transcriptome unevenness among plants. Total RNA from 4 plants was extracted with RNeasy (Qiagen, Hilden, Germany). The total RNA was subjected to further analysis. mRNA-seq library construction and sequencing were performed at Hokkaido System Science Co., Ltd. (Sapporo, Japan) in accordance with the manufacturer’s instructions by using an Illumina (San Diego, CA) TruSeq RNA Sample Prep Kit and 100-bp single-end protocol on a HiSeq2000 system (Illumina).
sample comment
DRS005718
sample name
Control 24 h
title