SAMD00010754
External Id
Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
INSDC center name
2015-03-16T00:00:00Z
INSDC first public
2015-03-15T20:20:40Z
INSDC last update
DRS012724
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS012724
SRA accession
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
This sample is total RNA in the HeLa cells that was prepared as a positive control.Approximately 1μg RNA was used to sequence an RNA-seq library, using mRNA-seq Sample Preparation Kit (Illumina) according to manufacturer’s protocol. HeLa cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% Fetal Bovine Serum and antibiotics at 37oC at 5% CO2 in a humidified incubator.siRNAs were transfected using Lipofectamine RNAiMAX (Invitrogen), according to the instructions from the manufacturer. Following the siRNA duplex transfection (final concentration 10nM), cells were harvested for 72h after the transfection.
sample comment
DRS012724
sample name
RNA_siCTRL_1
title