LIBEST_028116
External Id
SAMN01924514
External Id
King Saud University
INSDC center name
2013-02-23T23:05:04.253Z
INSDC first public
2015-05-20T15:03:03.170Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Sukary
cultivar
Pooled total RNA of young leaves, needles and fruits at different growth stages of a single female date palm tree (variety Sukary) was used for ds cDNA synthesis and amplification. RNA was isolated using Invitrogen's Concert Plant RNA Reagent (Life Technologies, Grand Island, NY, USA), Following isolation RNA was sent to Evrogen (Moscow, Russia) for normalization and cDNA synthesis option (CS011-2B LEVEL 2B). cDNA species were cloned into pAL17.3 cloning vector (Evrogen, Moscow, Russia). Clones were sequenced using an ABI 3730xl DNA automatic capillary sequencer and the ABI Big Dye Terminator sequencing kit. Software codonecode aligner (CodonCode Corporation.Centerville, MA, USA) was used for base calling and vector removal. Low quality sequences were also omitted. Expressed sequenced tags (EST) obtained were screened for di, tri, tetra and penta simple sequence repeats (SSR) using batch primer3 software.
description
different growth stages
dev-stage
female flower, developed fruits, leaves, needles
organ
Phoenix dactylifera
http://purl.obolibrary.org/obo/NCBITaxon_42345
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
Phoenix dactylifera cultivar Sukary EST-SSR library
title