SAMN01991174
External Id
University of Utah
INSDC center name
2013-05-08T06:50:14.457Z
INSDC first public
2014-03-06T17:06:36.870Z
INSDC last update
SRS405718
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS405718
SRA accession
DNA was extracted using Gentra PUREGENE DNA Isolation Kit and sheared on COVARIS to a median 400 bp fragment. For all samples unmethylated lambda DNA was added to 1% final concentration (Promega Madison, WI 53711). Methylated paired-end adapters were ligated to sheared DNA according to the Illumina library preparation protocol. Sodium bisulfite conversion was performed using the EpiTect Bisulfite Kit (Qiagen, Valencia, CA 91355) with an additional incubation step at 60C for 120 min to increase conversion rate. Bisulfite treated DNA was then PCR amplified with 16 cycles and sequenced. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
description
sphere
http://www.ebi.ac.uk/efo/EFO_0004526
dev stage
PE: paired end, SE: single end
label
Danio rerio
http://purl.obolibrary.org/obo/NCBITaxon_7955
organism
Tubingen
strain
Generic sample from Danio rerio
title