LIBEST_028197
External Id
SAMN02146721
External Id
University of Hyderabad
INSDC center name
2013-05-18T23:05:06.040Z
INSDC first public
2015-05-20T15:03:18.120Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
An EST library of late leaf spot disease induced genes was generated from fungal treated leaves of wild peanut (Arachis diogoi). 45 day old seedlings were grown in greenhouse at 27 deg C with a 12/12 h light/dark photoperiod. Detached leaves were maintained in tray and were sprayed with 1 lac/ml spores of phaeosariopsis personata and leaf samples were collecteted at different time points viz. 24hr, 48hr, 72hr and 96hr. Total RNA was extracted from the leaves of treated and control samples and cDNA-AFLP was conducted. Products were cloned using pTZ57R (Fermentas) as cloning vector. This work was conducted under DBT project entitled Analysis of host pathogen interaction in groundnut.
description
45 days old seedling
dev-stage
Arachis diogoi
http://purl.obolibrary.org/obo/NCBITaxon_170720
organism
leaves
http://purl.obolibrary.org/obo/PO_0025034
tissue-type
Arachis diogoi cDNA-AFLP library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pTZ57R cloning vector
vector