SAMN01931867
External Id
Dalhousie University
INSDC center name
2014-03-01T06:47:04.847Z
INSDC first public
2015-04-09T07:39:53.893Z
INSDC last update
SRS398467
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS398467
SRA accession
Monoeukaryotic culture with accompanying bacteria. Total RNA was isolated from cells harvested by centrifugation using Tri-Zol reagent (Sigma-Aldrich, St Louis, Missouri, U.S.A.) using standard manufacturer's protocols. Poly(A) RNA was isolated through two rounds of Poly(A) selection and a paired-end cDNA library with a nominal insert size of ~477bp was constructed by Macrogen (Seoul, South Korea), following the ILLUMINA HISEQ 2000 RNA-seq library construction protocol (ILLUMINA, San Diego, California, U.S.A.). The cDNA library was then sequenced using a * of a multiplexed ILLUMINA HISEQ 2000 lane.
description
unclassified eukaryotes
http://purl.obolibrary.org/obo/NCBITaxon_42452
organism
Phylogenomics demonstrate that breviate flagellates are related to opisthokonts: implications for the origin of genes involved in multicellularity
title