SAMN02212480
External Id
INRA
INSDC center name
2013-06-24T00:00:00Z
INSDC first public
2019-09-18T14:42:15.033Z
INSDC last update
SRS453949
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS453949
SRA accession
adults
age
whole body
http://purl.obolibrary.org/obo/BTO_0001489
body site
parthenogenetic female
breeding system
Aphids were reared on broad bean Vicia faba at low density (less than five individuals per plant) to prevent the production of winged morphs. Parthenogenesis was maintained under a 16h photoperiod and a temperature of 18ÁC. Twenty asexual females were then directly frozen into liquid nitrogen and kept for subsequent RNA extractions. RNA extractions were then performed using the SV Total RNA Isolation System (Promega) according to manufacturerÍs instructions. 4 separate RNA extractions of 5 adult individuals were performed. RNA quality was checked on Bioanalyzer (Agilent) and quantified on Nanodrop (Thermo Scientific). Two samples made of a pool of 2 µg of two of the four independent RNA extractions were generated and were subsequently sent to GATC Company for RNA paired-end sequencing
description
Acyrthosiphon pisum
http://purl.obolibrary.org/obo/NCBITaxon_7029
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
LSR1
strain
whole body
http://purl.obolibrary.org/obo/BTO_0001489
tissue type
Pea aphid Parthenogenetic morphs
title