SAMN02086078
External Id
LIBEST_028182
External Id
School of Agriculture & Biology, Shanghai Jiao Tong University
INSDC center name
2013-04-28T23:05:05.890Z
INSDC first public
2015-05-20T15:03:15.740Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
cDNA library was derived from petals of fully open flowers of Tulipa fosteriana. cDNA synthesis was initiated using a oligo (dT) primer. Double-stranded cDNA was blunted, digested with EcoRI and MseI restriction endonuclease, ligated to EcoRI and MseI adaptors, using cDNA-AFLP to screen the different expression gene, and cloned into the pMD18-T simple vector.
description
fully open
dev-stage
E. coli DH5-alpha
lab-host
Tulipa fosteriana
http://purl.obolibrary.org/obo/NCBITaxon_93697
organism
EcoRI
re_1
petals
http://purl.obolibrary.org/obo/PO_0009032
tissue-type
Tulipa fosteriana petal cDNA library
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pMD18-T vector
vector