LIBEST_028219
External Id
SAMN02190951
External Id
Latvian State Forest Research Institute Silava
INSDC center name
2013-06-05T23:05:04.850Z
INSDC first public
2015-05-20T15:03:21.420Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Two year old scots pine seedlings were foliar-sprayed and soil drenched with 10 mkM abscisic acid solution. Scots pine needles was collected (after 24 h, 64 h and one week) and frozen in liquid nitrogen. RNA was isolated using a CTAB-based method followed by several purifications with the Turbo DNA-free kit (Ambion).Reverse transcription of the extracted RNA was performed with random hexamer primers using 1 mkg of RNA with the TaqMan reverse transcription Kit (Applied Biosystems). Differentially expressed fragments were ligated into pTZ57R/T vector with InsTAclone PCR cloning kit (Thermo Scientific) and then transformed by heat shock into E.coli competent cells. Sequencing was performed by Sanger's method from both sides with M13 primers and BigDye Terminator v3.1 Cycle sequencing Kit (Applied Biosystems). Plasmid sequence was removed.
description
needle
organ
Pinus sylvestris
http://purl.obolibrary.org/obo/NCBITaxon_3349
organism
Differential expression after treatment with abscisic acid.
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pTZ57R/T
vector