SAMN02028953
External Id
Biocant
INSDC center name
2013-09-23T07:44:08.697Z
INSDC first public
2014-06-03T16:22:30.383Z
INSDC last update
SRS410823
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS410823
SRA accession
B. xylophilus from USA (1 isolate), was grown in fungal culture (Botrytis cinerea. The total RNA was isolated from mixed stage cultures with the Trizol method. cDNA was synthesized with MINT cDNA synthesis kit (Evrogen, Moscow, Russia) to amplify selectively mRNA through polyA tails, and normalized according to the Duplex-Specific Nuclease-technology, following the instructions of the TRIMMER cDNA Normalization kit (Evrogen, Moscow, Russia). 454 pyrosequencing of each normalized library was performed according to 454 standard procedures.
description
Bursaphelenchus xylophilus
http://purl.obolibrary.org/obo/NCBITaxon_6326
organism
B. xylophilus isolate from USA
title