SAMN01993129
External Id
J. Craig Venter Institute
INSDC center name
2013-03-28T10:01:20.003Z
INSDC first public
2014-06-09T18:20:28.773Z
INSDC last update
SRS405334
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS405334
SRA accession
embryonic stem cells ROSA26 (A. McMahon, Harvard)
breed
Mouse embryonic stem cells expressing an Enhanced Yellow Fluorescent Protein (EYFP) transgene from one ROSA26 locus and LacZ from the other ROSA26 locus, as well as tamoxifen-inducible CRE recombinase from untargeted viral integration, were maintained on a mouse embryonic fibroblast feeder layer by daily replacement of mES media [KO-DMEM (Invitrogen, San Diego, CA) containing 15% knock-out serum (Sigma, St. Louis, MO), 1X glutaMAX (Gibco, San Diego, CA), 1 X non-essential amino acids (Gibco), 55 μm 2-mercaptoethanol (Gibco), and 1000 U/mL mLIF (Millipore, Billerica, MA)]. These cells were a gift from A. McMahon (Harvard) (Hayashi and McMahon 2002; Mao et al. 2005).
description
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Mus musculus embryonic stem cells ROSA26
title