SAMN02179234
External Id
Stanford University
INSDC center name
2013-07-06T00:00:00Z
INSDC first public
2021-02-28T02:06:25.287Z
INSDC last update
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.5.0
NCBI submission package
sea water
http://purl.obolibrary.org/obo/CHEBI_15377
biome
1993-08-19
collection date
littoral zone
depth
Mature reproductive colonies of Botryllus schlosseri (Pallas) were collected from Santa Cruz and Monterey marinas (CA, USA). Hatched larvae were settled and raised in our mariculture facility as described (Boyd et al., 1986; De Tomasso et al., 1998). Long lived, highly regenerative colony 356a, was chosen to be sequenced. Subclones were starved for 48 hours prior to sampling (to minimize DNA contamination), and 150 individuals (zooids) were sampled for gDNA sequencing. To confirm that all zooids belonged to one genotype, a few zooids were taken from every sample set and screened for polymorphism via amplified fragment length polymorphism (AFLP) analysis as described in (Voskoboynik et al., 2008) and microsatellite loci analysis as described in (Stoner et al., 2002), confirming one genotype for 356a.
description
MIGS/MIMS/MIMARKS.water
env package
725Mb
estimated size
marina
feature
USA: Monterey, CA
geo loc name
eukaryote
http://edamontology.org/topic_2818
investigation type
DOI: 10.2307/1541383
isol_growth_condt
356a
http://www.ebi.ac.uk/efo/EFO_0002120
isolate
356a chromosome hybrid
label
36.60332 N 121.88944 E
lat lon
sea water
http://purl.obolibrary.org/obo/CHEBI_15377
material
100000
num_replicons
Botryllus schlosseri
http://purl.obolibrary.org/obo/NCBITaxon_30301
organism
diploid
http://purl.obolibrary.org/obo/CL_0000415
ploidy
The genome sequence of the colonial chordate, Botryllus schlosseri
project name
sexual/asexual
propagation
356a
http://www.ebi.ac.uk/efo/EFO_0002120
strain
Botryllus schlosseri gDNA from colony 356a
title