SAMN02215179
External Id
USDA/ARS/NADC/FSEPRU
INSDC center name
2013-11-16T00:00:00Z
INSDC first public
2019-07-21T02:04:42.815Z
INSDC last update
SRS455165
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.host-associated
NCBI submission model
MIMS.me.host-associated.5.0
NCBI submission package
SRS455165
SRA accession
swine intestinal tract
biome
2010-01-14
collection date
Keywords: GSC:MIxS;MIMS:5.0
description
MIGS/MIMS/MIMARKS.host-associated
env package
USA: Ames, Iowa
geo loc name
94 days old
host age
intestinal contents
host body product
pig
host common name
TechStart 17-25 (Kent Feeds, Muscatine, IA) containing ASP250 (chlortetracycline 100 g/ton, sulfamethazine 100 g/ton, penicillin 50 g/ton) ad libitum until euthanization (17 continuous days)
host diet
not fasted before euthanasia
host last meal
grower pig
host life stage
female
http://purl.obolibrary.org/obo/PATO_0000383
host sex
9825
host taxid
offspring of sow A
host_family_relationship
ambient
host_growth_cond
cecum
http://purl.obolibrary.org/obo/UBERON_0001153
host_tissue_sampled
metagenome
investigation type
swine
label
42°02?05?N 93°37?12?W
lat lon
contents of the cecum
material
pig metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1176744
organism
medicated (17 continuous days)
perturbation
Swine gut microbial ecology along the gut transect
project name
blend 5 g material in PBS, centrifuge at 250xg to reduce plant material, then centrifuge the supernatant at 10,000g to pellet the cells, followed by bead beating the resuspended pellet for bulk DNA extraction (kit by MoBio)
samp mat process
up to 5 grams feces/gut contents
samp_size
1 to 6 months
samp_store_dur
-20 degrees Celsius
http://purl.obolibrary.org/obo/UO_0000027
samp_store_temp
Pig 28
specific host
MIMS Environmental/Metagenome sample from Sus scrofa domesticus
title