LIBEST_028202
External Id
SAMN02178844
External Id
Waksman Institute, Rutgers University
INSDC center name
2013-05-25T23:05:03.100Z
INSDC first public
2015-05-20T15:03:18.763Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Landoltia punctata cDNAs were reversed transcribed from polyA-selected mRNAs. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pTriplEX2. Recombinant plasmids were transformed into E. coli strain DH5a.
description
mixed stages
dev-stage
E. coli DH5a
lab-host
whole plant
http://purl.obolibrary.org/obo/UBERON_0000468
organ
Landoltia punctata
http://purl.obolibrary.org/obo/NCBITaxon_50518
organism
SfiI
re_1
WSSP Duckweed cDNA library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pTriplEX2
vector