LIBEST_028230
External Id
SAMN02206259
External Id
Instituto de Biotecnologia, Universidad Autonoma de Mexico (UNAM)
INSDC center name
2013-09-04T23:05:04.147Z
INSDC first public
2015-05-20T15:03:23.180Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
A cDNA library was constructed from total RNA extracted from a single telson of the Egyptian scorpion Scorpio maurus palmatus. The purified mRNA was used to make the full length cDNA library of Scorpio maurus palmatus. The cDNA was digested using restriction enzyme SfiI. Fractions containing cDNA of the desired size were pooled and concentrated by ethanol precipitation. The purified cDNA was ligated into pDNR-LIB vector and transformed into electrocompetent Escherichia coli DH5alpha. Random colonies were selected and cultured in appropriate culture medium. Purified plasmids were single-pass sequenced on an automatic sequencer using the standard primers T7 and M13rev. ApE-A Plasmid Editor Program v.0.45 was used to examine the trace files and the vector sequences were removed. All high quality single pass sequences were submitted to the EST database to get the accession numbers.
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev-stage
Scorpio palmatus
http://purl.obolibrary.org/obo/NCBITaxon_1662106
organism
venom gland
tissue-type
cDNA library of Scorpio maurus palmatus venom gland
title