SAMN02211813
External Id
Chair for Microbial Ecology, Technische Universität München
INSDC center name
2014-07-20T00:00:00Z
INSDC first public
2019-06-20T16:33:19.262Z
INSDC last update
SRS450549
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS450549
SRA accession
For spinach medium, whole spinach leaves were homogenized (Agienda Agricola Pistelle, Kaufland, Germany) on ice using an Ultraturrax D50. The mush was centrifuged (1 h, 30,000×g, 5°C), decanted, filtered (2.5 µm pore size), centrifuged (2 h, 30,000×g, 5°C), decanted and sterile filtered (0.2 µm). After 5 h of growth, we harvested the cells at 6.0 x 108 cfu/ml.
growth condition and treatment
late logarthmic, early stationary phase
growth phase
Escherichia coli O157:H7 str. EDL933
http://purl.obolibrary.org/obo/NCBITaxon_155864
organism
whole organisms
http://purl.obolibrary.org/obo/UBERON_0000468
source_name
EDL933
strain
spinach juice technical replicate 1
title