SAMN02146722
External Id
LIBEST_028198
External Id
Beijing Forestry University
INSDC center name
2013-06-19T23:05:04.107Z
INSDC first public
2015-05-20T15:03:18.247Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Constructed from pooled aerial parts of A. mongolicus seedlings at different stages of cold treatment and water deficit. An equal amount of plant samples from every time point were pooled for total RNA extraction. Synthesized cDNA was digested with asymmetrical SfiI restriction enzyme and ligated into SfiI-digested vector pBluescript II SK, which was modified and contains the asymmetrical SfiI sites in the MCS and facilitates directional cloning. Blue/white plaque selection following incubation of an aliquot of the library. Blue/white plaque selection following incubation of an aliquot of the library showed a 95.5% recombinant rate with titers of 1.01E+6.
description
two-week-old seedlings
dev-stage
Ammopiptanthus mongolicus
http://purl.obolibrary.org/obo/NCBITaxon_126911
organism
SfiI A
re_1
SfiI B
re_2
aerial tissues
http://www.ebi.ac.uk/efo/EFO_0001901
tissue-type
Ammopiptanthus mongolicus cold- and drought-responsive library
title
pBluescript II SK with modified asymmetrical SfiI sites between EcoRI site and NotI site in the MCS region
vector