SAMN01991176
External Id
University of Utah
INSDC center name
2013-05-08T06:50:14.490Z
INSDC first public
2014-03-06T17:06:36.940Z
INSDC last update
SRS405725
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS405725
SRA accession
Maternal haploid embryos were generated according to standard procedure (Westerfield, 2000), using sperm from ÂgoldenÂ homozygous males gol b1/b1 as a marker. DNA from sphere-stage maternal haploid, and sphere-stage control IVF diploids was extracted using phenol-chloroform and bisulfite converted using the EpiTect Bisulfite Kit (Qiagen, Valencia, CA 91355), followed by PCR amplification of 15 promoter regions. Genomic library was prepared according to Illumina's standard procedure and sequenced on Illumina MiSeq. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
description
sphere
http://www.ebi.ac.uk/efo/EFO_0004526
dev stage
PE: paired end, SE: single end
label
Danio rerio
http://purl.obolibrary.org/obo/NCBITaxon_7955
organism
Tubingen
strain
Generic sample from Danio rerio
title