SAMN02212482
External Id
INRA
INSDC center name
2013-06-24T11:24:18.943Z
INSDC first public
2013-07-01T07:17:13.287Z
INSDC last update
SRS454061
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS454061
SRA accession
adults
age
whole body
http://purl.obolibrary.org/obo/BTO_0001489
body site
Aphids were reared on broad bean Vicia faba at low density (less than five individuals per plant) to prevent the production of winged morphs. The production of sexual individuals was initiated by transferring larvae from a 16h to a 12h photoperiod at the same temperature of 18ÁC. Two generations later, sexual females and males were observed. A total of 20 adult males were then directly frozen into liquid nitrogen. RNA extractions were then performed using the SV Total RNA Isolation System (Promega) according to manufacturerÍs instructions. 4 separate RNA extractions of 5 adult individuals were performed. RNA quality was checked on Bioanalyzer (Agilent) and quantified on Nanodrop (Thermo Scientific). Two samples made of a pool of 2 µg of two of the four independent RNA extractions were generated and were subsequently sent to GATC. Company for RNA paired-end sequencing.
description
Acyrthosiphon pisum
http://purl.obolibrary.org/obo/NCBITaxon_7029
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
LSR1
strain
whole body
http://purl.obolibrary.org/obo/BTO_0001489
tissue type
Pea aphid female morphs
title