SAMN01985847
External Id
LIBEST_028139
External Id
Sugarcane Breeding Institute
INSDC center name
2014-01-01T00:44:18.767Z
INSDC first public
2015-05-20T15:03:08.120Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Co 93009
cultivar
The work was initiated to identify differentially expressed genes at 72h in red rot resistant sugarcane cultivar Co 93009 in response to Colletotrichum falcatum. Total RNA was isolated from redrot resistant Co 93009 and redrot susceptible CoC 671 stalk tissues collected at 72h after C.falcatum inoculation. Total RNA sample of Co 93009 collected at 72h after pathogen inoculation was used as tester.Total RNA sample of CoC 671 collected at 72h after C. falcatum inoculation and 0th h sample of Co 93009 were pooled together and used as driver. Driver mRNA was subtracted from tester by hybridization,the subtracted product was amplified by two step nested PCR, cloned and sequenced to generate ESTs conferring red rot resistance in the cultivar Co 93009 at 72h.
description
Six months old cane stalk inoculated with C. falcatum
dev-stage
E.coli Omnimax 2T1 supercompetent cells
lab-host
Saccharum hybrid cultivar Co 93009
http://purl.obolibrary.org/obo/NCBITaxon_672235
organism
Nco I
re_1
Pst I
re_2
hermaphrodite
http://purl.obolibrary.org/obo/PATO_0001340
sex
stalk (stem)
tissue-type
LIBEST_Sugarcane x Colletotrichum falcatum 72h forward SSH cDNA library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEMT Easy vector
vector