SAMN01985844
External Id
LIBEST_028136
External Id
Sugarcane Breeding Institute
INSDC center name
2014-01-01T00:44:18.557Z
INSDC first public
2015-05-20T15:03:07.670Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Co 93009
cultivar
The work was initiated to identify differentially expressed genes in red rot resistant cultivar in response to the devastating pathogen Colletotrichum falcatum. Total RNA was isolated from redrot resistant Co 93009 and redrot susceptible CoC 671 stalk tissues collected at 0, 6, 12, 24, and 48h after C.falcatum inoculation. Total RNA samples of Co 93009 collected at 6, 12, 24, and 48h after pathogen inoculation were pooled together and used as tester. Total RNA samples of CoC 671 collected at 0, 6, 12, 24, and 48h after C. falcatum inoculation and 0th h sample of Co 93009 were pooled together and used as driver. Driver mRNA was subtracted from tester by subtraction and hybridization, the subtracted product was amplified by two step nested PCR, cloned and sequenced to generate ESTs conferring red rot resistance related defense responses in the cultivar Co 93009.
description
Six months old sugarcane stalk inoculated with C. falcatum
dev-stage
E.coli Omnimax 2T1 supercompetent cells
lab-host
Saccharum hybrid cultivar Co 93009
http://purl.obolibrary.org/obo/NCBITaxon_672235
organism
Nco I
re_1
Pst I
re_2
hermaphrodite
http://purl.obolibrary.org/obo/PATO_0001340
sex
stalk (stem)
tissue-type
LIBEST_Sugarcane x Colletotrichum falcatum forward subtracted cDNA library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEMT Easy vector
vector