SAMD00000571
External Id
Northeast Agricultural University
INSDC center name
2016-05-08T22:07:54Z
INSDC first public
2016-05-08T09:07:54Z
INSDC last update
DRS016219
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS016219
SRA accession
Glycine max
http://purl.obolibrary.org/obo/NCBITaxon_3847
organism
Specific length amplified fragment sequencing (SLAF-seq) is a recently developed high-resolution strategy for large scale de novo discovery and genotyping of single nucleotide polymorphisms. SLAF-seq was employed in this study to obtain sufficient markers to construct a high-density genetic map for soybean. In total, 33.10 Gb of data containing 171,001,333 pair-end reads were obtained. The average sequencing depth was 42.29 in the male parent, 56.63 in the female parent, and 3.92 in each progeny. In total, 164,197 high-quality SLAFs were detected, of which 12,577 SLAFs were polymorphic, and 5,308 of the polymorphic markers met the requirements for use in constructing a genetic map. The final map included 5,308 markers on 20 linkage groups and was 2,655.68 cM in length, with an average distance of 0.5 cM between adjacent markers. To our knowledge, this map has the shortest average distance of adjacent markers for soybean. We report here the first high-density genetic map for soybean. The map was constructed using a recombinant inbred line population and the SLAF-seq approach, which allowed the efficient development of a large number of polymorphic markers in a short time.
sample comment
DRS016219
sample name
Raw Data by SLAF-seq
title