SAMD00009632
External Id
Arima lab., Department of Informative Genetics, Environment and Genome Research Center, Graduate School of Medicine, Tohoku University
INSDC center name
2013-10-21T00:00:00Z
INSDC first public
2014-11-12T03:29:17Z
INSDC last update
DRS003829
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS003829
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Embryos on 129/svj (female) x JF1 (male) backgroud were produced by in vitro fertilization (IVF) and recovered at E13.5. The brain was dissected from E13.5 embryos and total RNA was prepared using RNeasy mini kit (Qiagen). Four micrograms of total RNA was used for library construction using TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturerÃ¢ÂÂs protocol. Briefly, poly-A containing mRNAs were purified using poly-T oligo-attached magnetic beads. The purified mRNAs were fragmented using divalent cations under elevated temperatures, and then converted to dsDNA by two rounds of cDNA synthesis using reverse transcriptase and DNA polymerase I. After an end repair process, DNA fragments were ligated with adaptor oligos. The ligated products were amplified using 15 cycles of PCR to generate RNA-seq library. Library integrity was verified by Bioanalyzer DNA1000 assay (Agilent technologies). Sequencing was performed in 39bp paired end mode using Genome Analyzer IIx (Illumina).
sample comment
DRS003829
sample name
IVF Brain-3
title