SAMD00003521
External Id
Nakayama lab, division of cell proliferation, Tohoku university graduate school of medicine
INSDC center name
2013-11-28T00:00:00Z
INSDC first public
2014-11-12T03:28:49Z
INSDC last update
DRS011939
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS011939
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
For comprehensive analysis of Ras-dependent changes in H3K27me content, we performed ChIP-seq analyses at various times after Ras induction. We sampled NIH 3T3 cells at 0, 2, 4, 7, and 12 days after infection with the H-Ras(G12V) retroviral vector. ChIP-seq libraries were prepared from ~40 ng each of ChIP and input DNA with the use of a TruSeq DNA LT Sample Prep Kit (Illumina, San Diego, CA). Libraries were clonally amplified in a flow cell and sequenced with the use of HiSeq Control Software 1.5 (Illumina) and a 48-nucleotide paired-end sequence. Image analysis and base calling were performed with the use of Real Time Analysis (RTA) 1.13 software.
sample comment
DRS011939
sample name
NIH 3T3 cells (0 days after introduction of Ras)
title