SAMN02342652
External Id
NIAMS/NIH
INSDC center name
2013-12-26T06:46:32.070Z
INSDC first public
2014-06-10T14:22:26.040Z
INSDC last update
SRS479293
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS479293
SRA accession
6-8 weeks
http://purl.obolibrary.org/obo/UO_0000034
age
CH12
cell line
splenic B cells
http://purl.obolibrary.org/obo/CL_0000785
cell type
Resting splenic B cells were isolated from 6-8 week-old wild-type C57BL6/J mice with anti-CD43 Microbeads (anti-Ly48; Miltenyi Biotech) and were activated for 48-60 h with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml; Sigma) and 0.5 μg ml−1 of anti-CD180 (RP105) antibody (RP/14, BD Pharmingen). Aicda enhancer E2 was deleted using TALENs approach.
description
Switchable IgM+àIgA+ murine CH12-F3 Ly-1+ B cell lymphoma line was maintained and passaged every 2 days in RPMI 1640 supplemented with 10% FBS (ATCC), 1% penicillin/streptomycin (Invitrogen), 55 uM 2-b mercaptoethanol (Invitrogen). All cells were maintained at 37°C and 5% CO2 in a humidified incubator. Aicda enhancer E2 was deleted using TALENs approach.
description
Aicda enhancer 2 deletion
genotype
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
activated B cells with Aicda Enhancer 2 deletion (Mus musculus)
title