SAMN02361099
External Id
Southern Cross Plant Science, Southern Cross University
INSDC center name
2013-09-23T12:00:28.210Z
INSDC first public
2014-06-09T18:30:36.477Z
INSDC last update
SRS486800
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.5.0
NCBI submission package
SRS486800
SRA accession
Poaceae
biome
5/1/2008
collection date
AR1
cultivar
Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice- like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction with high throughput sequencing of genes for key domestication traits. While M. stipoides has previously been identified as having potential as a new grain crop for human consumption, only a limited understanding of its genetic diversity and breeding system was available to aid the domestication process. Next generation sequencing of deeply-pooled target amplicons estimated allelic diversity of a selected base population at 24.1 SNP/kb and identified novel, putatively mutation induced polymorphisms at about 0.5 SNP/kb. High dose (LD97) ethylmethane sulfonate treatment was applied without inducing sterility in this polyploid species. Forward and reverse genetic screens identified beneficial alleles for the domestication trait, seed-shattering. Unique phenotypes observed in the M2 population suggest the potential for rapid accumulation of multiple beneficial traits without recourse to a traditional cross-breeding strategy. This approach may be applicable to other wild species, unlocking their potential as new food, fibre and fuel crops.
description
880Mb
estimated size
Cultivation
feature
Australia
geo loc name
eukaryote
http://edamontology.org/topic_2818
investigation type
http://nativeseeds.com.au/Weeping-Grass%2C-Microlaena-Stipoides.php
isol_growth_condt
37°48?49?S 144°57?47?E
lat lon
seed
http://purl.obolibrary.org/obo/PO_0009010
material
30000
num_replicons
Microlaena stipoides
http://purl.obolibrary.org/obo/NCBITaxon_66030
organism
Tetraploid
http://purl.obolibrary.org/obo/PATO_0001382
ploidy
Accelerated Domestication of Australian Native Grasses
project name
High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title