SAMD00000691
External Id
Department of Reproductive Biology
INSDC center name
2014-11-17T00:00:00Z
INSDC first public
2014-11-16T19:41:36Z
INSDC last update
DRS011759
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS011759
SRA accession
Mus musculus musculus
http://purl.obolibrary.org/obo/NCBITaxon_39442
organism
ES cells carrying a doxycycline-inducible Kdm4b transgene was created using a piggyBac transposon-based expression system from the NCH.4.6 male mouse ES cell line (C57B6/N x C57B6/N) with normal karyotype, and named K4B-ES cells.K4B-ES cells were cultured in the presence or absence of doxycycline, and subjected to fixed chromatin preparation. The fixed chromatin samples (Dminus and Dplus) were used for chromatin immuno-precipitation using anti-histone H3K9me3 polyclonal antibody (Abcam ChIP-grade ab8898). ChIP-Seq libraries were prepared for the immuno-precipitated DNA as well as the input DNA of Dminus and D plus samples using the NEBNextÂ® ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (NEB) according to manufacturer's instructions.
sample comment
DRS011759
sample name
musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
sub species
K4B-ES_Dminus_input
title