SAMN02211817
External Id
Chair for Microbial Ecology, Technische Universität München
INSDC center name
2014-07-20T00:00:00Z
INSDC first public
2019-06-20T16:33:19.372Z
INSDC last update
SRS450553
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS450553
SRA accession
Cattle feces: The number of cultivatable bacteria of cattle feces was determined by serial platings on LB-agar plates after 12h at 37°C. The cattle feces was subsequently inoculated with 1000-fold number of EHEC, pre-grown in 1 L LB to stationary phase. When the bacteria had reached stationary phase, they were harvested by centrifugation and resuspended in 7 ml ¼-concentrated Ringer. We added this suspension to 10 g of cattle feces and mixed it thoroughly. After 6 h at 37°C, bacterial cells were harvested by adding 90 ml cold ¼ Ringer, shaking for 10 s, sedimentation for 30 s, decanting, and centrifugation.
growth condition and treatment
late logarthmic, early stationary phase
growth phase
Escherichia coli O157:H7 str. EDL933
http://purl.obolibrary.org/obo/NCBITaxon_155864
organism
whole organisms
http://purl.obolibrary.org/obo/UBERON_0000468
source_name
EDL933
strain
feces
http://purl.obolibrary.org/obo/NCIT_C13234
title