LIBEST_028251
External Id
SAMN02264026
External Id
Northwest A&F University
INSDC center name
2013-07-25T23:05:03.527Z
INSDC first public
2015-05-20T15:03:26.747Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Suppression subtractive hybridization was performed using a PCR-Select cDNA subtraction kit (Clontech, USA) according to the manufacturer's protocol. Purified PCR products from the second PCR reaction were cloned into pGEM-T Easy vector (Promega) and transformed into E. coli DH5alpha competent cells. Clones were randomly picked out and sequenced by Sanger sequencing with M13 primers. Successfully sequenced ESTs were vector and adaptor trimed and assembled using Vector NTI Advantage 11 software resulting in 126 contigs (EST ID containing TwHfcontig) and 419 singlets (EST ID containing TwHf)
description
Tripterygium wilfordii
http://purl.obolibrary.org/obo/NCBITaxon_458696
organism
adventitious root
tissue-type
Suppression subtractive hybridization library for enrichment of secondary metabolites biosynthesis genes
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEM-T Easy
vector