SAMD00013909
External Id
Takagi laboratory
INSDC center name
2015-04-01T00:00:00Z
INSDC first public
2015-03-31T20:36:27Z
INSDC last update
DRS016030
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS016030
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 Â°C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 Â°C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols.
sample comment
DRS016030
sample name
C57BL/6J
http://www.ebi.ac.uk/efo/EFO_0000606
strain
RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 1
title