SAMN02230654
External Id
LIBGSS_039123
External Id
Bielefeld University, CeBiTec
INSDC center name
2013-12-30T00:00:00Z
INSDC first public
2018-03-07T15:55:57.022Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Boerner
cultivar
BAC library provided by JKI Institute for Grapevine Breeding Geilweilerhof. Randomly sheared genomic DNA was inserted into pIndigoBAC5 at the HindIII site. Average insert size is about 90 KB. Clones were arrayed into 384well plates, and BACs were end-sequenced from both ends using an ABI3730XL. Sequencing primers were fwd (5-GGATGTGCTGCAAGGCGATTAAGTTGG-3) and rev (5-CTCGTATGTTGTGTGGAATTGTGAGC-3) for plate 1-52 and 55. Plates 53,54 and 56-112 were sequenced using modified sequencing primers, fwd (5-CGACGGCCAGTGAATTGTAATACG-3) and rev (5-CTCGTATGTTGTGTGGAATTGTGAGC-3).
description
E.coli DH10B
lab-host
Vitis riparia x Vitis cinerea
http://purl.obolibrary.org/obo/NCBITaxon_2018007
organism
sticky
re_1
sticky
re_2
Grape leaves
tissue-type
IGS-SCF-1083 Vitis-hybrid 'Boerner' BAC genomic library
title
pIndigoBAC5
vector