LIBEST_028125
External Id
SAMN01938886
External Id
Zoological Research Museum Alexander Koenig
INSDC center name
2013-10-29T23:05:04.320Z
INSDC first public
2015-05-20T15:03:05.873Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Collected_by: Torsten H. Struck; collection_date: 17-Mar-2008; collection_site: Deep channel of the German bight near the North Sea island Helgoland, Germany; Total RNA was extracted from one complete individual. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. An amplified cDNA library was constructed using the MINT UNIVERSAL cDNA synthesis kit (Evrogen). cDNA was size fractioned and directional cloned using the vector pDNR-LIB. Clones containing cDNA inserts were sequenced from the 5' end on the automated capillary sequencer systems ABI 3730 XL (Applied Biosystems, Darmstadt, Germany) and MegaBace 4500 (GE Healthcare, Munich, Germany) using BigDye chemistry. Sequencing was performed at the MPI for Molecular Genetics Berlin.
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev-stage
Owenia fusiformis
http://purl.obolibrary.org/obo/NCBITaxon_6347
organism
whole organism
http://purl.obolibrary.org/obo/UBERON_0000468
tissue-type
Owenia fusiformis adult cDNA library
title
pDNR-LIB
vector