SAMD00024617
External Id
Okayama University
INSDC center name
2017-12-15T00:00:00Z
INSDC first public
2017-12-15T08:03:53Z
INSDC last update
DRS056572
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS056572
SRA accession
Methylobacterium sp. 22A was grown in 100-ml methanol medium prepared in 500-ml shaking flask. The media contain 0.5% succinate. In log phase of the growth (OD600=0.3-0.4), the cells were harvested by centrifugation (9000 rpm, 4 C, 5 min), and frozen by liquid nitrogen. The cells were subjected to total RNA extraction with hot phenol extraction procedure (Babst et al., 1996, Mol Microbiol 19:827-839). Then ribosomal RNA was depleted by using Ribo-Zero rRNA removal kits (Gram-negative bacteria) (Epicentre) according to the manufacturer's protocol.
description
Methylobacterium sp. MA-22A
http://purl.obolibrary.org/obo/NCBITaxon_1577727
organism
Methylobacterium_sp. 22A_RNAseq_S
sample name
MA-22A
strain
Methylobacterium_sp. 22A_RNAseq_S
title