SAMN06321076
External Id
Oscar Aparicio, Biological Sciences, University of Southern California
INSDC center name
2017-03-10T00:00:00Z
INSDC first public
SRS1969794
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
SRS1969794
SRA accession
anti-BrdU
antibody
Late G1-phase
cell cycle
BrdU-IP DNA was obtained by arresting cells with alpha-factor to OD ~0.5 in YEPD, releasing into YEPD, hydroxyurea, and BrdU. DNA was extracted and sonicated using Covaris S2, and BrdU-incorporated DNA was incubated with anti-BrdU to obtain BrdU-IP material, as described in Ostrow et al., 2017.
extract protocol
OTHER
library strategy
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Liquid cultured S. cerevisiae
source_name
ZOy21
strain
fkh1Δ BrdU-IP-Seq rep2
title