SAMN06435839
External Id
Jeremy Boss, Microbiology and Immunology, Emory University
INSDC center name
2019-05-18T00:00:00Z
INSDC first public
2019-05-18T01:32:17.940Z
INSDC last update
SRS1999211
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS1999211
SRA accession
Be2
be type
Purified naive B cells were co-cultured for 6 days in the presence of IL-2 (50U/ml) and IL-21 (10ng/ml) with allogeneic in vitro generated Th2 effectors (0.6x10​6 cells/ml, ratio 5B:1T) in Iscove's DMEM supplemented with penicillin (200 microg/ml), streptomycin (200migrog/ml), gentamicin (40microg/ml), 10% FBS, transferrin (50microg/ml) and insuline (5microg/ml).
culture conditions
C11
donor
Negative
http://purl.obolibrary.org/obo/CL_0002371
igd status
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
male
http://purl.obolibrary.org/obo/PATO_0000384
sex
CD19+ CD38mid CD27lo IgD- CD4-
sorting markers
B cells
http://purl.obolibrary.org/obo/CL_0000236
source_name
C11_Be2_IgD_neg
title