12a-30.tccgcttattgatatgc_CGAGAGATAC.TCC
External Id
SAMN06274079
External Id
mothur
INSDC center name
2017-01-26T00:00:00Z
INSDC first public
2021-02-28T03:59:53.520Z
INSDC last update
SRS1941872
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.miscellaneous
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.miscellaneous.5.0
NCBI submission package
SRS1941872
SRA accession
2014-01
collection date
Forest soil (1-10 cm depth) taken after understory removal, replicate 3
description
Forest soil (1-10 cm depth) taken after understory removal, replicate 3
description
forest soil
env biome
forest soil
env feature
soil
env_material
Chile: Puyehue National Park
geo loc name
40.79 S 72.95 W
lat lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
soil 1-10 cm after understory removal
sample title
Sequencing was performed on a 454 GS FLX Titanium system with the fungal primers ITS1F/ITS4. PCR conditions: 95�C for 5 min; 94�C for 60 s / 50�C for 60 s / 72�C for 90 s (25 cycles); 72�C for 10min. PCR reaction (total 25 �l): 1�PCR buffer with 1.8 mM MgCl2, 0.2 mM dNTPs, 1.25 U High-Fidelity polymerase (Roche), 0.2 �M of each primer, 0.5�l 3% BSA, 20 ng of template DNA. Sample purification: Gel and PCR Cleanup Kit (Macherey Nagel). Emulsion PCR: 94�C for 4 min; 94�C for 30 s / 60�C for 10 min (50 cycles).
seq_methods
soil 1-10 cm after understory removal
title