SAMN06271910
External Id
University of Missouri Columbia
INSDC center name
2017-01-25T00:00:00Z
INSDC first public
2021-02-28T03:59:37.310Z
INSDC last update
SRS1999190
INSDC secondary accession
live
INSDC status
MIGS.ba
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIGS.ba.soil.5.0
NCBI submission package
SRS1999190
SRA accession
2015-02
collection date
15-20 cm
http://purl.obolibrary.org/obo/UO_0000015
depth
Keywords: GSC:MIxS;MIGS:5.0
description
272 m
elev
Agricultural Region
env biome
Farm
env feature
Farm Soil
env_material
USA: Columbia, Missouri, 10 m east of stable on side of low hill
geo loc name
Five grams of each soil sample were diluted in 50 ml ISP2 medium and the inoculated medium was placed in a 30 degree C shaker at 100 rpm for 60 min. Ten ml of three different dilutions (10-1, 10-2, 10-3) were prepared in sterile saline solutions 0.85% (wt/vol) NaCl. Then, 100 microliters of each dilution was cultured on ISP 2 agra medium containing 100 mg/L Nystatin as antifungal agent. Plates were incubated at 30 degrees C and monitored each 24 h for 14 days.The isolated Streptomyces sp. was then grown in a fermentation medium composed of the following in one liter (tryptone 5g, yeast extract 5g, glucose 10g, anhydrous K2HPO4 3g, KH2PO4 1g, NaCl 3g, MgSO4.7H2O 0.5g, anhydrous CaCO3 0.5g) pH 7.0 Manivasagan, et al., 2013
isol_growth_condt
38.869722 N 92.185278 W
lat lon
Streptomyces sp. MOE7
http://purl.obolibrary.org/obo/NCBITaxon_1961713
organism
In Preparation
ref_biomaterial
Dropped soil sample into twirl pak and the sample was refrigerated within 30 minutes.
samp mat process
Sterilized Spatula
samp_collect_device
MOE7
strain
Extracellular polysaccharide-producing soil isolate Streptomyces sp. ME7.
title