SAMN06464482
External Id
ENCODE
INSDC center name
2017-03-02T00:00:00Z
INSDC first public
SRS2044597
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
SRS2044597
SRA accession
5 day
age
H9
http://www.ebi.ac.uk/efo/EFO_0003045
cell type
embryonic
dev stage
ENCDO222AAA
donor id
healthy
http://purl.obolibrary.org/obo/PATO_0000461
health state
Bradley Bernstein, Broad
lab
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
59
passage number
stem cell
http://purl.obolibrary.org/obo/CL_0000034
sample type
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
ENCODE biosample ENCBS703ZDS: HES5::eGFP BAC transgenic human ES cells (H9; WA-09; Wicell) expressing GFP under the HES5 promoter, Cells were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) (Globalstem). Undifferentiated ES cells were maintained in medium containing DMEM/F12, 20% KSR, 1mM Glutamine, 1% Penicillin/Streptomycin, non essential amino acids and beta-mercaptoethanol. Undifferentiated ES cells were purified with pluripotency markers Tra-1-60 and PE-conjugated SSEA-3 (BD Pharmingen).
title