SAMN06231251
External Id
Institute of Disease Control and Prevention
INSDC center name
2017-06-01T00:50:56.680Z
INSDC first public
2017-06-01T00:50:56.680Z
INSDC last update
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
15
age
ATCC
biomaterial provider
For more than 30 years, scientists have studied many biological processes in HepG2 cells, publishing some 20,865 articles along the way. This cell is particularly abnormal compared with non-cancer human cell lines, as well as compared with the human reference sequence. However, most genomic studies in HepG2 cells have used the human reference sequence, which is a serious impediment in corresponding genetic level researches using HepG2 cell line such as genomic variation, virus-host genetic interaction and the validating targets of siRNAs, CRISPR-Cas9 and other reagents against a specific sequence. Here, we used Next Generation Sequencing (NGS) to define the HepG2 genome, revealing the true extent of genetic abnormalities at nucleotide resolution, which are valuable resources for the continued use of HepG2 cells in biomedical research.
description
HB-8065
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
male
http://purl.obolibrary.org/obo/PATO_0000384
sex
liver
http://purl.obolibrary.org/obo/UBERON_0002107
tissue
human hepatocellular carcinoma cell line HepG2 ATCC isolate HB-8065
title