SAMN06321061
External Id
Oscar Aparicio, Biological Sciences, University of Southern California
INSDC center name
2017-03-10T00:00:00Z
INSDC first public
SRS1969808
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
SRS1969808
SRA accession
Late G1-phase
cell cycle
4C DNA was created by arresting cells with alpha factor, crosslinking with formaldehyde, isolating intact chromatin, digesting with XbaI, ligating with T4 DNA Ligase, digesting with MseI, ligating with T4 DNA Ligase, performing PCR with primers adjacent to and emanating from ARS305, and sonicating with Covaris S2, as described in Knott et al., 2012 and Ostrow et al., 2017.
extract protocol
OTHER
library strategy
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Liquid cultured S. cerevisiae
source_name
CVy138
strain
fkh2Δ 4C-Seq rep1
title