SAMN06276231
External Id
National Heart Lung and Blood Institute
INSDC center name
2017-11-21T12:37:27.640Z
INSDC first public
2017-11-21T12:37:27.640Z
INSDC last update
SRS1948292
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS1948292
SRA accession
11-14 days
age
Susan T. Harbison
biomaterial provider
National Heart Lung and Blood Institute
birth location
We created the Sleep Advanced Intercross Population (SAIP) from the 5 longest-sleeping and 5 shortest-sleeping lines of the Drosophila Genetic Reference Panel (DGRP). The lines we used were DGRP-235, DGRP-313, DGRP-335, DGRP-338, and DGRP-379 (long sleepers); and DGRP-38, DGRP-310, DGRP-365, DGRP-808, and DGRP-832 (short sleepers). These lines are maximally homozygous; the genome-wide heterozygosity is less than 5%. DGRP-38 is heterozygous for the In(3R)K inversion; DGRP-313 is homozygous for the In(2L)t inversion; DGRP-335 is heterozygous for the In(3R)Mo inversion; DGRP-338 is heterozygous for the In(2L)t and In(2R)NS inversions; and the remaining lines have the standard karyotype for all common polymorphic inversions. DGRP-310, DGRP-335, DGRP-338, DGRP-365, and DGRP-832 were infected with Wolbachia pipientis; the remaining lines were not. All ten lines were crossed in a full diallel design, resulting in 100 crosses. Two virgin females and two males from the F1 of each cross were randomly mixed together and placed into 20 bottles, with 10 males and 10 females in each bottle. At each generation thereafter, 20 virgin females and 20 males from each bottle were randomly mixed with the other flies, and a new generation of 20 bottles was reared. Each generation of random mating had a census population size of 800. The genomes of these flies were allowed to recombine in this manner for 21 generations, resulting in an outbred population of flies.
breeding_method
Laboratory of Systems Genetics
collected by
Pool of 35 female flies from mix of sleep advanced intercross population at generation 0 of selection
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev stage
USA: Bethesda, MD
geo loc name
Sleep selection S1-F-0 of SAIP from DGRP
isolate
Drosophila melanogaster
http://purl.obolibrary.org/obo/NCBITaxon_7227
organism
Short Sleep Duration
http://www.ebi.ac.uk/efo/EFO_0000537
phenotype
whole organism
http://purl.obolibrary.org/obo/UBERON_0000468
sample type
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
whole body
http://purl.obolibrary.org/obo/BTO_0001489
tissue
Short sleeper population 1, generation 0, female
title
Selection for short sleep duration
treatment