SAMN06321067
External Id
Oscar Aparicio, Biological Sciences, University of Southern California
INSDC center name
2017-03-10T00:00:00Z
INSDC first public
SRS1969803
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
SRS1969803
SRA accession
asynchronous
cell cycle
total RNA was isolated from 10 mL cultures grown in YEPD to OD600 ~1 using Ribopure Yeast Kit (ThermoFisher AM1926). cDNA libraries were created using SuperScript III First-Strand Synthesis (ThermoFisher #18080051) with oligo dT primers followed by NEBNext Second Strand Synthesis (NEB E6111S).
extract protocol
RNA-Seq
http://www.ebi.ac.uk/efo/EFO_0002770
library strategy
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Liquid cultured S. cerevisiae
source_name
ZOy45
strain
fkh2-dsm fkh1Δ RNA-Seq rep1
title