SAMD00012669
External Id
RIKEN Omics Science Center, Yokohama Institute, RIKEN
INSDC center name
2018-12-28T00:00:00Z
INSDC first public
2018-12-28T08:05:36Z
INSDC last update
DRS002036
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS002036
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
24 nanoCAGE/CAGEscan libraries were prepared from total RNAs of Fluorescence-activated cell sorting (FACS) sorted parvalbumin-positive neurons ranging from 30-127 ng, where four treatments including two HDACi (TSA and VPA) with their vehicle controls, two time points after each treatment (2h and 2d) were compared. Three biological replicates for each combination of treatment and time point were prepared. In order to sequence multiplex libraries at a time, a six nucleotide barcode system was introduced. Briefly, the barcodes were introduced inside the template-switching primer and incorporated at the 5Ã¢ÂÂ²-end of the nanoCAGE libraries through reverse transcription. After cDNA synthesis, 24 libraries were pooled into single library and adapted for sequencing with an Illumina Genome analyzer IIx.
sample comment
DRS002036
sample name
NanoCAGE/CAGEscan libraries from total RNAs of Fluorescence-activated cell sorting(FACS) sorted parvalbumin-positive neurons.
title