SAMD00160559
External Id
Pharmaceuticals Research Unit,Discovery Technology Laboratory I,Mitsubishi Pharma Corporation
INSDC center name
2019-02-01T00:00:00Z
INSDC first public
2019-01-31T23:04:31Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
U251
http://www.ebi.ac.uk/efo/EFO_0006498
cell line
Human glioblastoma
cell type
The knockdown vector was constructed by using a shRNA expression retroviral vector pSINsi-hU6 (TAKARA Bio, Shiga, Japan). The most efficient target sequence for RNA interference was selected among six. U251 cells were exposed to the virus pools produced by transfection of the vector into Amphopack293 packaging cells. The transfected cells were subcultured at an appropriate density in fresh DMEM containing 0.5 mg/ml. The G418-resistant cell pools were readily established within 2 weeks.
description
gene_knock_in
http://www.ebi.ac.uk/efo/EFO_0004963
genetic_modification
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
IGFBP2 knockdown U251 subline
sample name
IGFBP2 knockdown subline
strain
IGFBP2 knockdown U251 subline
title