SAMD00119015
External Id
Laboratory of Genome Regeneration, Center for Epigenetic Disease, The University of Tokyo, Institute of Molecular and Cellular Biosciences
INSDC center name
2019-01-03T00:00:00Z
INSDC first public
2019-01-03T08:01:37Z
INSDC last update
DRS085401
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS085401
SRA accession
Replicate sample_1. Parental yeast strain with low rDNA copy, YPD culture cross-linked by 1% formaldehyde at 30degree Celsius for 30 minutes. 2x10^9 cells were lysed by YTZ-beads, and the extracts were sheared by Covaris S220 to 100bp average. After pelleting, soluble fractions were used for immunoprecipitation with 10micro-g of anti-HA F-7 antibody and ProteinG dynabeads. Immunoprecipitates were reverse-crosslinked and purified. Sequencing libraries were prepared by KAPA Hyperprep kit and TruSeqHT-compatible adaptors.
description
MATa fob1&Delta;0 RDN1-15 his3&Delta;1 leu2&Delta;0 met15&Delta;0 ura3&Delta;0
genotype
MATa
mating type
Saccharomyces cerevisiae BY4741
http://purl.obolibrary.org/obo/NCBITaxon_1247190
organism
biological replicate 1
replicate
UAF_ChIP007
sample name
YTT157
strain
WT_RDN1-15_IP1
title