SAMN10230265
External Id
Indian Institute of Chemical Biology
INSDC center name
2021-10-11T00:50:08.840Z
INSDC first public
2021-10-11T00:50:08.840Z
INSDC last update
SRS3905926
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS3905926
SRA accession
48 hpf
age
Dr. Mahendra Sonawane, TIFR
biomaterial provider
Sonal Patel, NCCS
collected by
Scaffold Matrix Attachment Region 1, SMAR1 is a characterised nuclear matrix binding protein. It is thought to be a mammalian protein that plays role in a various nuclear functions. In the present study, we have identified and characterised SMAR1 homologue in zebrafish (zSMAR1) of 2.43 Kb length consisting of 13 exons that encodes a 508 amino acid long protein. zSMAR1 shares 66% homology to mouse SMAR1. Morpholino antisense oligonucleotide (MO) mediated knock-down of zSMAR1 in fish embryos led to embryonic malformations - smaller head size, pericardial edema and a linear heart tube phenotype indicating its role in fish embryogenesis. The present RNA-Seq data is generated on Illumina NextSeq 500 platform yielding 75 nucleotide long reads in paired-end format. The samples used for sequencing are Control MO injected and MO targeting zSMAR1 injected zebrafish embryos.
description
Long-pec, 48hpf
dev stage
Danio rerio
http://purl.obolibrary.org/obo/NCBITaxon_7955
organism
Tubingen
strain
Whole embryo
http://purl.obolibrary.org/obo/UBERON_0000922
tissue
Danio rerio SMAR1-Morpholino mediated knockdown
title
SMAR1 Morpholino
treatment