Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_02 ACACGT
1.VPA_2h_01
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_30 CACATA
10.VEH/vpa_2d_01
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_31 CACGAT
11.VEH/vpa_2d_02
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_38 CGACGT
12.VEH/vpa_2d_03
Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_43 CGATAC
13.TSA_2h_01
Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_50 CTGCGT
14.TSA_2h_02
Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_55 GAGACG
15.TSA_2h_03
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_63 GAGTGA
16.VEH/tsa_2h_01
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_66 GCTATA
17.VEH/tsa_2h_02
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_68 GCTCGT
18.VEH/tsa_2h_03
Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_74 GTACGT
19.TSA_2d_01
Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_04 ACAGAT
2.VPA_2h_02
Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_78 GTAGTG
20.TSA_2d_02
Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_79 GTATAC
21.TSA_2d_03
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_86 TATCGT
22.VEH/tsa_2d_01
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_90 TATGTG
23.VEH/tsa_2d_02
Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_91 TCGACG
24.VEH/tsa_2d_03
Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_06 ACAGTG
3.VPA_2h_03
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_07 ACATAC
4.VEH/vpa_2h_01
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_14 AGTCGT
5.VEH/vpa_2h_02
Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_16 AGTGAT
6.VEH/vpa_2h_03
Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_18 AGTGTG
7.VPA_2d_01
Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_22 ATCGAT
8.VPA_2d_02
Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_27 ATCTGA
9.VPA_2d_03
SAMD00010004
External Id
RIKEN Omics Science Center, Yokohama Institute, RIKEN
INSDC center name
2018-12-28T00:00:00Z
INSDC first public
2018-12-28T08:04:21Z
INSDC last update
DRS012047
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS012047
SRA accession
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
24 nanoCAGE/CAGEscan libraries were prepared from total RNAs of Fluorescence-activated cell sorting (FACS) sorted pyramidal neurons ranging from 30-100 ng, where four treatments including two HDACi (TSA and VPA) with their vehicle controls, two time points after each treatment (2h and 2d) were compared. Three biological replicates for each combination of treatment and time point were prepared. In order to sequence multiplex libraries at a time, a six nucleotide barcode system was introduced. Briefly, the barcodes were introduced inside the template-switching primer and incorporated at the 5′-end of the nanoCAGE libraries through reverse transcription. After cDNA synthesis, 24 libraries were pooled into single library and adapted for sequencing with an Illumina Genome analyzer IIx.
sample comment
DRS012047
sample name
NanoCAGE/CAGEscan libraries from total RNAs of Fluorescence-activated cell sorting(FACS) sorted pyramidal neurons.
title