SAMD00115857
External Id
Gunma Prefectural Fisheries Experiment Station
INSDC center name
2018-10-02T00:00:00Z
INSDC first public
2018-10-02T01:18:30Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Gunma Prefectural Fisheries Experiment Station
collected by
2008
collection date
Suppression subtractive hybridization (SSH) library was performed with the PCR-Select DNA Subtraction Kit (Takara Bio USA) following the manufacture's protocol. mRNA from kidney of Flavobacterium psychrophilum-injected (virulent GMA0330 or avirulent NCIMB 1947 strains in ayu) and phosphate buffered saline (PBS)-injected fish was used to make tester or driver cDNAs, respectively. Two SSH libraries were performed: the first SSH cDNA library (GP library) was constructed using cDNA derived from virulent GMA0330-injection fish as the tester and cDNA derived from PBS-injected fish as the driver, the second SSH cDNA library (GN library) was constructed using cDNA derived from virulent GMA0330-injection fish as the tester and cDNA derived from avirulent NCIMB 1947-injection fish as the driver.
description
juvenile
http://purl.obolibrary.org/obo/UBERON_0000112
dev stage
Bacterial coldwater disease
disease
Japan:Gunma
geo loc name
Flavobacterium psychrophilum infected
health_state
Plecoglossus altivelis altivelis
http://purl.obolibrary.org/obo/NCBITaxon_281464
organism
Plecoglossus altivelis altivelis EST (SSH library)
sample name
pooled male and female
sex
altivelis
sub species
Kidney
http://purl.obolibrary.org/obo/UBERON_0002113
tissue type
Flavobacterium psychrophilum infected ayu SSH library (GP or GN library)
title