SAMD00027925
External Id
Tokyo Medical and Dental University Medical Research Institute Gene Expression
INSDC center name
2019-03-31T00:00:00Z
INSDC first public
2019-03-31T09:03:31Z
INSDC last update
DRS092202
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS092202
SRA accession
Hidehito KUROYANAGI
biomaterial provider
2014-03-18
collection date
The worm strain used was KH1752. This is a mutant worm strain. The genotype is unc-75 (yb1701) I. Embryos were obtained by bleaching synchronized gravid adults. The embryos were incubated for 18 hours at 20 degree Celsius in M9 buffer. The synchronized L1 worms were then fed with E. coli strain OP50 suspended in S-complete medium for 3 hours at 20 degree Celsius. The L1 worms were cleaned by washing with the M9 buffer and total homogenate was prepared by grinding the worms in liquid nitrogen. Cytoplasmic fraction (clear supernatant) was prepared by centrifuging the total homogenate. Total RNAs were extracted from the cytoplasmic fraction by using Sepasol I Super (nacalai tesque) and then purified by using RNeasy Plus Mini kit plus DNase I (Qiagen).
description
L1
http://www.ebi.ac.uk/efo/EFO_0001084
dev stage
lst-3 (yb1719) IV
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
6_lst-3_L1_cytoplasm
sample name
hermaphrodite
http://purl.obolibrary.org/obo/PATO_0001340
sex
KH2124
strain
lst-3 (yb1719) L1 cytoplasmic fraction [RNAseq]
title