SAMD00030053
External Id
Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences , University of Tokyo
INSDC center name
2019-04-09T00:00:00Z
INSDC first public
2019-04-09T09:04:29Z
INSDC last update
DRS092476
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS092476
SRA accession
wild-type. Exponential phase. strand-nonspecific mRNA-seq. This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using TruSeq RNA library prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
description
Helicobacter pylori 26695
http://purl.obolibrary.org/obo/NCBITaxon_85962
organism
26695_exp
sample name
26695
strain
26695_exp
title