SAMD00032032
External Id
Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences , University of Tokyo
INSDC center name
2019-04-09T00:00:00Z
INSDC first public
2019-04-09T09:04:29Z
INSDC last update
DRS092475
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS092475
SRA accession
PIK40 (deltaHPP12_1497::aphA).Exponential phase. strand-specific mRNA-seq. This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using SureSelect - Strand specific RNA library kit (Agilent Technologies). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
description
Helicobacter pylori P12
http://purl.obolibrary.org/obo/NCBITaxon_570508
organism
PIK40_exp_SS
sample name
PIK40_exp_SS
title