SAMD00196021
External Id
Reseach Center for Allergy and Immunology, RIKEN
INSDC center name
2019-12-06T00:00:00Z
INSDC first public
2019-12-06T06:00:04Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
no information available
age
B cell
http://purl.obolibrary.org/obo/CL_0000236
cell type
Tonsillar B cells were negatively selected using the B cell isolation kit II (Miltenyi Biotech) according to the manufacturer's instructions. The isolated cells were &gt;99.5% B cells. The resulting tonsillar B cells were stained for CD38 and IgD expression and memory B cells were identified as CD38-/IgD- cells. Separation of FCRL4-positive and negative memory B cells was performed using a MoFlow FACS sorter (Dako Cytomation, Fort Collins, CO) after co-staining with biotinylated F(ab')2 fragments of FCRL4 antibodies.
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev stage
normal
http://purl.obolibrary.org/obo/PATO_0000461
disease
no information available
genotype
no information available
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
2_Tonsil FcRL4 positive B cells
sample name
no information available
sex
no information available
strain
tonsil
http://purl.obolibrary.org/obo/UBERON_0002372
tissue
2_Tonsil FcRL4 positive B cells
title