SAMN14511029
External Id
Gp0405607
External Id
DOE Joint Genome Institute
INSDC center name
2020-03-31T00:00:00Z
INSDC first public
2020-05-05T10:17:16.290Z
INSDC last update
SRS6590524
INSDC secondary accession
live
INSDC status
MIGS.ba
NCBI submission model
MIGS.ba.5.0
NCBI submission package
SRS6590524
SRA accession
Sinorhizobium meliloti FSM-MA WT bacteroids of Medicago sativa proximal nodule sections methylation detection; Sterilized seeds were germinated on Kalys agar in the dark. Seedlings were inoculated with the bacteria, and then transferred to pots containing a perlite:sand mixture. Plants and grown for 35 or 37 days in a greenhouse with occasional watering with a 1 g L-1 nutrient solution (PlantProd solution [N-P-K, 0-15-40; Fertil]). At the end of the growth period, nodules were harvested, manually sectioned at the pink-white interface, and stored at -80 until further use. Bacteroids were enriched from the nodule material using Percol gradient centrifugation prior to DNA isolation.
description
Sinorhizobium meliloti
http://purl.obolibrary.org/obo/NCBITaxon_382
organism
FSM-MA
strain
Sinorhizobium meliloti FSM-MA methylation detection - FSM-MA-GD-ZIII-1
title