SAMN14345097
External Id
University of Cambridge
INSDC center name
2020-03-10T00:00:00Z
INSDC first public
2020-03-10T12:01:10.750Z
INSDC last update
SRS6296662
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS6296662
SRA accession
over stage 3 (see https://www.arabidopsis.org/portals/education/growth.jsp)
dev stage
United Kingdom: Cambridge
geo loc name
In the last generation of selection we collected self seed from all 200 individuals of each of the 18 populations. We then pooled together 5 seeds from each (total of 1000 seeds per population) and grew them on compost and a size hole punch of leaf tissue was used as material for DNA. All leaf samples were ground together for DNA extraction. In this way, each of the original 200 individuals should have contributed a similar amount of tissue to the final pool.
growth_protocol
Pool of all ~200 individuals from the last generation of selection (generation 10) from STABILISING selection on HIGH NITRATE replicate B - repeat to achieve >100x depth of coverage
isolate
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
Population derived from the Arabidopsis MAGIC lines, themselves derived from 19 accessions
population
Pooled DNA
sample type
leaf (plant ontology term PO:0025034)
tissue
Bulk of individuals from STABILISING selection on HIGH NITRATE replicate B - repeat
title