SAMN14433833
External Id
University of Oregon
INSDC center name
2020-09-22T18:39:01.400Z
INSDC first public
2020-09-22T18:39:01.400Z
INSDC last update
SRS6362239
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS6362239
SRA accession
Eric U. Selker; N3756
biomaterial provider
University of Oregon; Selker lab
collected by
2010/2017
collection date
Genomic DNA was isolated using a protocol modified from (Oakley et al, 1987); if Bulk Segregant analysis was to be performed, the genomic DNA from 15 to 50 dim progeny (characterized by a methylation-sensitive restriction enzyme Southern blot) from a cross between the dim mutant (Oakridge) and the SNP mapping strain Mauriceville were equally pooled, and this pool of genomic DNA was subjected to whole genome sequencing. Whole genome sequencing was done with the Nextera kit (Illumina) following manufacturers’ protocol, except that after the PCR amplification step, libraries were purified using a Qiagen MinElute kit (PCR purification protocol), fragments between 350 - 550bp were size selected from a 2% TAE gel following gel electrophoresis, and final libraries were purified using a Qiagen MinElute kit (Gel Extraction protocol).
description
mat A sad-1 his-3
genotype
N3756
isolate
A
mating type
Neurospora crassa
http://purl.obolibrary.org/obo/NCBITaxon_5141
organism
one to three; asexual culture growth
passage_history
whole organism
http://purl.obolibrary.org/obo/UBERON_0000468
sample type
OR74a
strain
WGS of Neurospora crassa dim mutants: N2977 mutagenesis parent
title