SAMN14432513
External Id
USDA-ARS-Beltsville Sustainable Agricultural Systems Laboratory
INSDC center name
2020-03-24T00:00:00Z
INSDC first public
2020-05-01T00:46:26.370Z
INSDC last update
SRS6577438
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS6577438
SRA accession
Mani Subramanian lab, University of Iowa
biomaterial provider
University of Iowa Center for Biocatalysis and Bioprocessing
collected by
2007
collection date
The CES strain was isolated from soil at the University of Iowa Center for Biocatalysis and Bioprocessing using an enrichment procedure with caffeine as sole carbon and nitrogen source. Prior to isolation, coffee grounds had been amended to the soil for ten days. The soil was then collected and incubated in M9 minimum medium with .25 percent caffeine as described previously previously (Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.) The strain was initially characterized as Pseudomonas sp. Strain CES (for Coffee Enriched Soil) based on 16S rRNA sequencing and FAME analysis. CES uses the N-demethylation pathway for caffeine breakdown.
description
USA: Iowa, University of Iowa, Iowa City
geo loc name
Chi Li Yu
identified by
coffee ground-enriched garden soil
isolation source
Pseudomonas sp. CES
http://purl.obolibrary.org/obo/NCBITaxon_2719586
organism
cell culture
http://purl.obolibrary.org/obo/BTO_0000214
sample type
CES
strain
Microbe sample from Pseudomonas sp.
title