SAMN14395431
External Id
OMICS Laboratory, Department of Biotechnology, University of North Bengal
INSDC center name
2021-03-16T00:50:09.743Z
INSDC first public
2021-03-16T00:50:09.743Z
INSDC last update
SRS6332512
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS6332512
SRA accession
2017-10-01
collection date
Metagenomic DNA was isolated from processed cow dung using PowerSoil DNA Isolation kit (MO BIO Laboratories, Inc). DNA amplification of the V3 region of the 16S rRNA gene was carried out using Ion 16S Metagenomics kit (Thermo). Purification of the PCR amplicons was done using Purelink Quick Gel Extraction kit (Invitrogen). Amplicon quantification was performed using Qubit 4 Fluorometer (Thermo Fisher Scientific). Library preparation was carried out using Ion Plus Fragment Library kit (Thermo Fisher Scientific), in which, end-repairing and purification of the pooled amplicons was done first. Then adapter-ligation and nick-repairing and purification of the products were done. Determination of library concentration was done using Ion Universal Library Quantitation kit (Thermo). Template preparation was done using Ion-PGM Hi-Q OT2 kit. Library was sequenced in Ion Torrent platform (Ion S5 system) using Ion PGM Hi-Q sequencing kit.
design description
D_amp_6.IonXpress_019.R_2019_01_16_17_56_33_user_S5-0466-6-D_Serise_WGS
filename
fastq
filetype
India: Siliguri
geo loc name
Eisenia fetida
http://purl.obolibrary.org/obo/NCBITaxon_6396
host
Ion Torrent S5
instrument model
processed cow dung
isolation source
26.7072 N 88.3558 E
lat lon
D_amp_6.IonXpress_019.R_2019_01_16_17_56_33
library id
single
library layout
PCR
library selection
METAGENOMIC
library source
AMPLICON
library strategy
manure metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1792145
organism
ION_TORRENT
platform
Bacterial metataxonomy of earthworm bed for rearing Eisenia fetida prepared using processed cow dung
title
Bacterial metataxonomy of earthworm bed for rearing Eisenia fetida prepared using processed cow dung
title