SAMD00189825
External Id
Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
INSDC center name
2021-01-29T00:00:00Z
INSDC first public
2021-01-29T08:17:11Z
INSDC last update
DRS171752
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS171752
SRA accession
Synchronized L1 larvae of KH1668: smg-2 (yb979) I were fed with an E. coli strain OP50 in S-complete medium supplemented with 4 mM 4-thiouracil (4TU) (Tokyo Chemical Industry Co., Ltd.) for 6 hours at 20 degree Celsius. The worms were harvested and washed with M9 and total RNAs were extracted from the whole bodies by using RNeasy Plus Mini (QIAGEN). 4TU-labeled RNAs were biotinylated with MTSEA biotin-XX (Biotium) and purified by using Streptavidin Mag Sepharose (GE-healthcare). Ribosomal RNAs were then removed with Ribo-Zero Gold rRNA Removal Kit (Epidemiology) (illumina) and the remaining RNAs were subjected to direct RNA sequencing with Nanopore MinION platform.
description
L1
http://www.ebi.ac.uk/efo/EFO_0001084
dev stage
smg-2 (yb979) I
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
Nonsense-mediated mRNA decay (NMD)-deficient
phenotype
w12457
sample name
KH1668
strain
whole
http://purl.obolibrary.org/obo/NCIT_C25326
tissue
w12457_KH1668_L1_4TU-6h_biotin_Ribo-Zero
title