SAMN14345081
External Id
University of Cambridge
INSDC center name
2020-03-10T00:00:00Z
INSDC first public
2020-03-10T12:01:07.937Z
INSDC last update
SRS6296667
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS6296667
SRA accession
10 day seedling
dev stage
United Kingdom: Cambridge
geo loc name
In the last generation of selection we collected self seed from all 200 individuals of each of the 18 populations. We then pooled together 5 seeds from each (total of 1000 seeds per population) and grew them in liquid media for 10 days. We used 50ml liquid ATS media, shaken at 40RPM in a tissue culture room with 16 hour light and a temperature of 22/18 ºC during day/night cycles. After 10 days, all seedlings were harvested and ground together for DNA extraction. In this way, each of the original 200 individuals should have contributed a similar amount of tissue to the final pool.
growth_protocol
Pool of all ~200 individuals from the last generation of selection (generation 10) from RANDOM selection on HIGH NITRATE replicate B
isolate
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
Population derived from the Arabidopsis MAGIC lines, themselves derived from 19 accessions
population
Pooled DNA
sample type
whole plant (plant ontology term PO:0005679)
tissue
Bulk of individuals from RANDOM selection on HIGH NITRATE replicate B
title