SAMN14395338
External Id
Yangzhou University
INSDC center name
2020-03-17T00:00:00Z
INSDC first public
2020-03-18T01:31:53.163Z
INSDC last update
SRS6331544
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS6331544
SRA accession
J774A.1
cell line
macrophage
http://purl.obolibrary.org/obo/CL_0000235
cell type
J774A.1 cells infected with Salmonella Enteritidis C50336-1
description
adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev stage
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
mixed culture
sample type
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
BALB/cN
strain
mouse blood
tissue
C50336-1
title
J774A.1 cells were seeded at a density of 5000000 cells per 10 cm dish. Overnight, cells were were pre-treated with 1 g/mL LPS for 5 h, and then infected with Salmonella Enteritidis C50336-1 at an MOI of 20:1 and spun onto the cells at 1000 rpm for 10 min. Cells were incubated at 37 for 1 h, and subsequently washed twice, incubated for an additional 3 h with Opti-MEM containing 50 g/mL genttamicin. Then the cells were collected with 1 mL Trizol. The total RNA was isolated, purified, and quantified.
treatment