SAMD00189811
External Id
Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
INSDC center name
2021-01-29T00:00:00Z
INSDC first public
2021-01-29T08:17:11Z
INSDC last update
DRS171758
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS171758
SRA accession
Nucleosomes with modified histone (Dimethyl H3 (Lys4)) were immunoprecipitated from the chromatin fraction of KH2421: smg-2 (yb979) I; sams-5 (gk147) IV; sams-1 (ok2946) X (described above) with mouse monoclonal antibody MABI0303 (MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., Nagoya, Japan) and the DNA was purified after proteinase K digestion of proteins at 55 degree Celsius and reverse crosslinking at 65 degree Celsius overnight.
description
L1
http://www.ebi.ac.uk/efo/EFO_0001084
dev stage
smg-2 (yb979) I; sams-5 (gk147) IV; sams-1 (ok2946) X
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
Nonsense-mediated mRNA decay (NMD)-deficient
phenotype
sams_H3K4me2
sample name
KH2421
strain
whole
http://purl.obolibrary.org/obo/NCIT_C25326
tissue
ChIPed DNA_smg-2;sams-5;sams-1_L1_anti-histone H3K4me2
title