SAMD00189810
External Id
Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
INSDC center name
2021-01-29T00:00:00Z
INSDC first public
2021-01-29T08:17:11Z
INSDC last update
DRS171757
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS171757
SRA accession
Synchronized L1 larvae of KH2421: smg-2 (yb979) I; sams-5 (gk147) IV; sams-1 (ok2946) X were fed with an E. coli strain OP50 in S-complete medium for 30 min at 20 degree Celsius. The worms were then washed with M9, frozen in liquid nitrogen and ground to powder with mortar and pestle. The worm powder was treated with 1% (final) formaldehyde for 10 min at 20 degree Celsius and cell nuclei were collected by centrifugation at 1,000 g. The chromatin was solubilized with Covaris M220 (Peak Incident Power (PIP) = 75W; Duty Factor = 20%; Cycles Per Burst (CPB) = 200; time = 1,800 seconds). The DNA was purified from the chromatin fraction after proteinase K digestion of proteins at 55 degree Celsius and reverse crosslinking at 65 degree Celsius overnight.
description
L1
http://www.ebi.ac.uk/efo/EFO_0001084
dev stage
smg-2 (yb979) I; sams-5 (gk147) IV; sams-1 (ok2946) X
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
Nonsense-mediated mRNA decay (NMD)-deficient
phenotype
sams_Input
sample name
KH2421
strain
whole
http://purl.obolibrary.org/obo/NCIT_C25326
tissue
ChIP Input DNA_smg-2;sams-5;sams-1_L1
title