SAMN18839306
External Id
Gastroenterology and Hepatology, Erasmus MC
INSDC center name
2021-04-24T00:00:00Z
INSDC first public
2021-04-24T05:05:16.420Z
INSDC last update
SRS8758458
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS8758458
SRA accession
Primary immortalized human Barrett's cell line
cell line
BAR-T cells were transduced with virus made using the the pTLCV2-HOXA13sgRNA plasmid. Mixed populations of transduced cells were plated at very low confluence, single cell clones could subsequently be isolated using glass cloning cylinders and low melting point agarose from Sigma-Aldrich, followed by DNA isolation using the Kleargene kit, followed by sequence verification with primers TILHOXA13R3 and Pre-HOXA13-FW2 flanking the sgRNA-site (Extended data table 4). Three cell lines in which both alleles were not affected were selected.
genetic modification
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
BAR-T cell line
source_name
BAR-T CONTROL 2
title