SAMN18740202
External Id
Nanjing Medical University
INSDC center name
2021-07-08T00:00:00Z
INSDC first public
2021-07-08T08:24:02.503Z
INSDC last update
SRS8707784
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS8707784
SRA accession
D30
age
Yan liu's lab of Nanjing medical university
biomaterial provider
IMR90-4
cell line
brain organoids
cell type
ATAC-seq
description
D30
dev stage
For ATAC-seq, organoids were dissociated to single cells. About 50000 single cells from each group were used for nuclei prep. First, we spun cells for 5min, and then we washed these cells once with cold 1x PBS buffer, which was followed by centrifugation for 5 min. Next, cells were lysed by cold lysis buffer (containing 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630) on ice and nuclei were pelleted by centrifugation at 500g for 10min.
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
female
http://purl.obolibrary.org/obo/PATO_0000383
sex
brain organoids
tissue
Human sample from Homo sapiens
title