SAMN18868287
External Id
University of California, Riverside
INSDC center name
2021-04-25T00:00:00Z
INSDC first public
2021-05-06T06:12:06.736Z
INSDC last update
SRS8771320
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.host-associated
NCBI submission model
MIGS.eu.host-associated.5.0
NCBI submission package
SRS8771320
SRA accession
2019-06
collection date
A. aggregata culture was a single spore isolated from a diseased Megachile rotundata cadaver collected in Logan, UT. DNA was extracted from culture using a salting out procedure adapted from Sambrook and Russel (2001).
description
rangeland biome [ENVO:01000247]
env_broad_scale
area of pastureland or hayfields [ENVO:01000891]
env_local_scale
animal-associated environment [ENVO:01001002 ]
env_medium
USA: Logan, Utah
geo loc name
Megachile rotundata
host
The cadaver was confirmed as infected with a single A. aggregata infection through the use of species-specific primers modified from James and Skinner (2004) and modified for real time PCR (See below; Klinger et al. 2015). Spore isolation and resulting hyphal growth used for DNA extraction was performed on V-8 media (James and Buckner 2004).
isol_growth_condt
individual cadaver
isolation source
41.7431067 N 111.8146146 W
lat lon
Ascosphaera aggregata
http://purl.obolibrary.org/obo/NCBITaxon_51564
organism
USDA-ARS PIBMSR Wild2
strain
Ascosphaera aggregata strain wild2 (MIGS Eukaryotic)
title