SAMN18836311
External Id
University of Wisconsin-Madison
INSDC center name
2021-04-22T00:00:00Z
INSDC first public
2021-04-22T16:55:16.620Z
INSDC last update
SRS8755375
INSDC secondary accession
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
SRS8755375
SRA accession
BIOQUAL
collected by
2021-01-29
collection date
BEI<USA>:70041598
culture collection
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA/CA_CDC_5574/2020 was isolated from a nasopharyngeal swab on December 29, 2020 in San Diego County, California, USA. NR-54011 lot 70041598 was produced by infecting Cercopithecus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™) with the deposited material and incubating in Eagle’s Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 4 days at 37°C with 5% CO2. Bioqual then conducted a fourth passage using Calu-3 cells.
description
USA: Maryland, Rockville
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
COVID-19
http://purl.obolibrary.org/obo/MONDO_0100096
host disease
Homo sapien
http://purl.obolibrary.org/obo/NCBITaxon_9606
isolate
not appliable
isolation source
39.0840 N 77.1528 W
lat lon
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
V(2)/V(1)/C(1) (Centers for Disease Control and Prevention/BEI Resources/BIOQUAL); V = Vero cells C= Calu-3 Cells
passage_history
biological replicate 2
replicate
USA/CA_CDC_5574/2020
strain
hCoV-19 Challenge Stock: 012921-1230_Rep2
title