SAMN18712239
External Id
Nanjing Univerisity of Chinese Medicine
INSDC center name
2021-04-12T00:00:00Z
INSDC first public
2021-04-12T05:01:09.277Z
INSDC last update
SRS8681533
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS8681533
SRA accession
8 weeks old
http://purl.obolibrary.org/obo/HsapDv_0000025
age
EA (2/100Hz, 0.5-1.5 mA) was applied to ipsilateral Zusanli (ST36) and Sanyinjiao (SP6) for 30 min, once a day for one week. The positioning of acupoints follows the standards of experimental acupuncture. ST36 is located in the anterior tibial muscle and about 3mm below the knee joint. SP6 is located approximately 3 mm proximal to the largest medial eminence of the posterior malleolus and between the Achilles tendon and the distal tibia. Sterile acupuncture needles (size: 0.30 × 5 mm [diameter:0.18 mm, length:10 mm]; made by Beijing Zhongyan Taihe Medical Instrument Co, Ltd., (Beijing, China) were inserted perpendicularly to a depth of 1 to 3 mm into the two acupoints. Han's Acupoint Nerve Stimulator (WQ1002F, Beijing, China) provides an electro-stimulation of 30 minutes at a frequency of 2/100 Hz and at an intensity level of 0.5-1.0-1.5 mA to produce slight twitches in the limbs. After EA, the mice were allowed to completely free from a self‐made binding instrument and further acclimatized in the cage. Mice from the EA group were treated by EA every afternoon for 7 days, whereas mice from the M+Sham group were subjected to sham EA.
description
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
biological replicate B5
replicate
mRNA
http://purl.obolibrary.org/obo/CHEBI_33699
sample type
male
http://purl.obolibrary.org/obo/PATO_0000384
sex
C57BL/6
http://www.ebi.ac.uk/efo/EFO_0004472
strain
brainstem
http://purl.obolibrary.org/obo/UBERON_0002298
tissue
BS-Pre-EA5(M+EA_mice)
title
Repeat morphine for 7 days (10mg/kg,i.p., twice per day)+ EA treatment
treatment