SAMD00204281
External Id
IQB, The University of Tokyo
INSDC center name
2021-06-24T00:00:00Z
INSDC first public
2021-06-24T09:10:22Z
INSDC last update
DRS191236
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS191236
SRA accession
BY-2 cells
cell line
miR390 treated small RNAs from in vitro recapitulation system (tobacoo cell free system)
description
incubated at 130 rpm by shaking in a dark at 27 degree celsius
growth condition
BY-2 cells were cultured in 100 ml the medium (KOH, pH 5.8) containing 0.46 g Murashige and Skoog Plant Salt Mixture (Wako), 3 g sucrose, 0.1 mg Thiamine hydrochloride, 10 mg myo-Inositol, 20 mg KH2PO4 and 0.2 g 2, 4-Dichlorophenoxyacetic Acid.
growth_protocol
Nicotiana tabacum
http://purl.obolibrary.org/obo/NCBITaxon_4097
organism
S3_miR390 only_small RNAs from in vitro recapitulation system
sample name
cell culture
sample type
miR390 only_small RNAs from in vitro recapitulate system
title
A lysate was prepared from evacuated BY-2 cells. We incubated miR390 duplexes, TAS3 mRNAs and SDE5 mRNAs in the lysate.
treatment