SAMD00202699
External Id
Life and Environmental Sciences, University of Tsukuba
INSDC center name
2021-06-14T00:00:00Z
INSDC first public
2021-06-14T09:03:50Z
INSDC last update
DRS189543
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS189543
SRA accession
The steamed Yamada-nishiki polished 50% were inoculated with spores of A. oryzae, 10^4 spores per rice 1 g, and incubated in lab dishes with papers containing 4 ml water to keep humidity at 30oC for 33 h. Temperature of the incubator was increased 34, 38, 42oC gradually each for 5 h, total 48 h. To isolate the total RNA from fungal cells in rice koji, rice koji were are frozen and homogenized using mortar and pestle, and then the total RNA is extracted using an RNA isolation kit (RNA Mini Kit, Zymo Biomics).
description
Aspergillus oryzae RIB40
http://purl.obolibrary.org/obo/NCBITaxon_510516
organism
Y50
sample name
Yamada-nishiki 50%
title