SAMN18953818
External Id
SIES College of Arts, Science and Commerce
INSDC center name
2021-07-01T00:51:58.007Z
INSDC first public
2021-07-01T00:51:58.007Z
INSDC last update
SRS8912247
INSDC secondary accession
live
INSDC status
Viral
NCBI submission model
Virus.1.0
NCBI submission package
SRS8912247
SRA accession
2016-04-05
collection date
In this study, Mycobacterium smegmatis MTCC 994 was used as the host for isolating the mycobacteriophage, labeled as J1 from a sewage sample obtained from Juinagar, Navi Mumbai, Maharashtra, India on April 5, 2016. The objective of this study was to isolate mycobacteriophages using a nonpathogenic strain Mycobacterium smegmatis and then test the resulting novel mycobacteriophages for their ability to infect M. tuberculosis and other species of Mycobacterium. The Mycobacterium smegmatis MTCC 994 strain used in this study is the original NIHJ1628 (ATCC 14468) designated strain, obtained in 1984 from National Institute of Health, Japan (Subramanyam et al., 1989). The strain was deposited at IMTECH, by V.R. Subramanyam. To determine the host diversity, the isolated J1 mycobacteriophage was tested upon four other Mycobacterium strains which were obtained from the Institute of Microbial Technology (IMTECH), Chandigarh, India i) Mycobacterium fortuitum subsp. fortuitum MTCC 993 ii) Mycobacterium kansasii MTCC 3058 iii) Mycobacterium avium subsp. avium MTCC 1723 iv) non-virulent Mycobacterium tuberculosis MTCC 300. The mycobacteriophage isolated from a sewage sample from Juinagar, Navi Mumbai; was found to have a diverse host range and was found to be effective in killing five different species of Mycobacterium. It was also found to be effective in inhibiting the growth of a virulent strain Mycobacterium tuberculosis H37Rv. The structural characterization of the isolated mycobacteriophage was done by Transmission Electron Microscopy. The mycobacteriophage morphology using TEM analysis was found to be nonenveloped, with an isometric capsid and a non-contractile short tail. Based on the guidelines of the International Committee on Taxonomy of Viruses ICTV 9th report, since the isolated phage is a tailed phage with dsDNA it belongs to the order Caudovirales. Based on the short tail morphology it can be classified as belonging to the family Podoviridae. The above study was carried out at the Department of Microbiology, S.I.E.S. College of Arts, Science and Commerce, Sion West, Mumbai, India. The effectiveness of the isolated J1 mycobacteriophage on the virulent strain of Mycobacterium tuberculosis H37Rv was performed at Microcare Laboratory and Tuberculosis Research Centre (Accredited by the Revised National TB Control Programme), Surat, Gujarat, India. Transmission Electron Microscopy was done at the electron microscopy facility, Advanced Centre for Treatment, Research and Education in Cancer, Kharghar.
description
India:Juinagar, Navi Mumbai (Thane district, Maharashtra)
geo loc name
Mycolicibacterium smegmatis
host
Mycobacterium
isolate
sewage
isolation source
Mycobacterium phage RSat-2021a
http://purl.obolibrary.org/obo/NCBITaxon_2831865
organism
Mycobacteriophage J1 from Mycolicibacterium smegmatis
title