SAMN18836315
External Id
University of Wisconsin-Madison
INSDC center name
2021-04-22T00:00:00Z
INSDC first public
2021-04-22T16:55:17.403Z
INSDC last update
SRS8755379
INSDC secondary accession
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
SRS8755379
SRA accession
BIOQUAL
collected by
2021-02-16
collection date
BEI<USA>:70041942
culture collection
NR-54009 lot 70041942 was produced by infecting Cercopithecus aethiops kidney epithelial cells with human signaling activation molecule (Vero-hSLAM) with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/South Africa/KRISP-K005325/2020 and incubating in Eagle’s Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 4 days at 37°C with 5% CO2. Cell lysate and supernatant was clarified by centrifuging at 1500 × g for 10 minutes at room temperature. Bioqual then conducted a fourth and fifth passage using Calu-3 cells.
description
USA: Maryland, Rockville
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
COVID-19
http://purl.obolibrary.org/obo/MONDO_0100096
host disease
Homo sapien
http://purl.obolibrary.org/obo/NCBITaxon_9606
isolate
not appliable
isolation source
39.0840 N 77.1528 W
lat lon
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
H(1)VE(2)/C(2) (African Health Research Institute/BEI Resources/BIOQUAL); H = H1299 cells overexpressing human ACE2 receptor (H1299-hACE2-E3); VE = Vero E6 cells C= Calu-3 Cells
passage_history
biological replicate 2
replicate
hCoV-19/South Africa/KRISP-K005325/2020
strain
hCoV-19 Challenge Stock: NR-54009-P5_Rep2
title