SAMN18839308
External Id
Gastroenterology and Hepatology, Erasmus MC
INSDC center name
2021-04-24T00:00:00Z
INSDC first public
2021-04-24T05:03:09.500Z
INSDC last update
SRS8758497
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS8758497
SRA accession
Primary immortalized human squamous esophageal cell line
cell line
The pEN_TmiRc3 vector was cut using AgeI and XbaI restriction sites, a gift from Iain Fraser (Addgene (Cambridge, USA) #25748). pSLIK-Venus was a gift from Iain Fraser (Addgene #25734), a version without HOXA13 was used for the control cell line. LGC Genomics (Teddington, UK) sequenced both plasmids. Next, plasmids were packaged into lentiviral particles following transfection in HEK293T cells with third generation packaging plasmids. The supernatant was collected and ultracentrifuged. EPC2-hTERT cells were transduced with the virus and Fluorescence-Activated Cell Sorted (FACS) for YFP (pSLIK-Venus) positive cells on the BD FACSCantoTM II that was bought from BD Biosciences (San Jose, USA). These cells were grown and analyzed as a cell pool. HOXA13 was induced by the addition of 1,25 µg/ml doxycycline to the culture medium. Overexpression was determined by qPCR according to scientific standards (data not shown).
genetic modification
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
EPC2-hTERT cell line
source_name
EPC2-hTERT CONTROL 4
title