SAMN18836317
External Id
University of Wisconsin-Madison
INSDC center name
2021-04-22T00:00:00Z
INSDC first public
2021-04-22T16:55:15.870Z
INSDC last update
SRS8755371
INSDC secondary accession
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
SRS8755371
SRA accession
BIOQUAL
collected by
2021-03-06
collection date
BEI<USA>:70041987
culture collection
NR-54974 lot 70041987 was produced by infecting Homo sapiens lung adenocarcinoma cells (Calu-3; ATCC® HTB-55™) with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/South Africa/KRISP-K005325/2020 and incubating in Eagle’s Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 5 days at 37°C with 5% CO2. Cell lysate and supernatant was clarified by centrifuging at 1500 × g for 10 minutes at room temperature and vialed (0.1 mL per vial). Then Bioqual conducted a fifth passage using Calu-3 cells.
description
USA: Maryland, Rockville
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
COVID-19
http://purl.obolibrary.org/obo/MONDO_0100096
host disease
Homo sapien
http://purl.obolibrary.org/obo/NCBITaxon_9606
isolate
not appliable
isolation source
39.0840 N 77.1528 W
lat lon
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
H(1)VE(2)/C(1)/C(1) (African Health Research Institute/BEI Resources/BIOQUAL); H = H1299 cells overexpressing human ACE2 receptor (H1299-hACE2-E3); VE = Vero E6 cells; C = Calu-3 cells
passage_history
biological replicate 2
replicate
hCoV-19/South Africa/KRISP-K005325/2020
strain
hCoV-19 Challenge Stock: 030621-750_Rep2
title