SAMN18765960
External Id
University of Minnesota
INSDC center name
2021-08-23T00:00:00Z
INSDC first public
2021-08-23T11:13:02.271Z
INSDC last update
SRS8715470
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.plant-associated
NCBI submission model
MIGS.eu.plant-associated.5.0
NCBI submission package
SRS8715470
SRA accession
2014-12-01
collection date
A. cochlioides isolate 103-1 was collected from the Breckenridge Lime Plots, MN (Plot No.103). It was isolated in December 2014 using soil baiting techniques at the Sugar Beet Plant Pathology (SBPP) lab in Crookston, MN. A. cochlioides isolate 103-1 was grown on PDA (potato dextrose agar) for one week. Three agar cubes, approximately 5 mm 3 were excised from the growth margin of PDA cultures, and used to inoculate 100 mL of 1/8th strength PDB (potato dextrose broth) in 250 mL Erlenmeyer flasks, which were placed on a culture shaker at low speed at room temperature. Isolate 103-1 mycelium was harvested from the 1/8th PDB cultures after 5 days of growth. Pure mycelium was isolated from the liquid PDB cultures.
description
temperate biome
env_broad_scale
agricultural field
http://purl.obolibrary.org/obo/ENVO_00000114
env_local_scale
agricultural soil
env_medium
USA: Minnesota
geo loc name
Beta vulgaris
http://purl.obolibrary.org/obo/NCBITaxon_161934
host
Soil baiting
isol_growth_condt
103-1
isolate
46.26 N 96.58 W
lat lon
Aphanomyces cochlioides
http://purl.obolibrary.org/obo/NCBITaxon_112091
organism
MIGS Eukaryotic sample from Aphanomyces cochlioides
title