characteristics.text: SAMN22608725
characteristics.characteristic: External Id
characteristics.text: U.S. Dept. of Agriculture, Agricultural Research Service, National Animal Disease Center
characteristics.characteristic: INSDC center name
characteristics.text: 2021-10-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-10-27T18:39:09.193Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS10748613
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS10748613
characteristics.characteristic: SRA accession
characteristics.text: 2015-10-09
characteristics.characteristic: collection date
characteristics.text: An overnight bacterial culture grown at 37°C in LB broth was diluted 1:100 (A600 = 0.10) into a low-glucose Dulbecco’s Minimal Eagles Medium (DMEM). After about 5.5 h of incubation at 37°C with shaking (250 rpm) to allow cultures to attain A600 ≈1.2, total RNA was isolated using RNeasy isolation kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). RNA was treated with DNase (TURBO DNA-free kit; ThermoFisher Scientific, Grand Island, NY). The DNase-treated RNA was treated with Ribo-Zero rRNA kit reagents according to the manufacturer’s instructions (Gram-negative bacteria; Illumina, Inc., San Diego, CA) to remove rRNA.
characteristics.characteristic: description
characteristics.text: USA: Iowa
characteristics.characteristic: geo loc name
characteristics.text: NADC 5570/86-24/6564
characteristics.characteristic: isolate
characteristics.text: Laboratory culture
characteristics.characteristic: isolation source
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.characteristic: sample type
characteristics.text: O157:H7
characteristics.characteristic: strain
characteristics.text: 6564 transcriptome control
characteristics.characteristic: title
characteristics.text: Control
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: treatment