characteristics.text: SAMN22567960
characteristics.characteristic: External Id
characteristics.text: University Leipzig
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-13T12:43:43.943Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-13T12:43:43.943Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS10748585
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS10748585
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: altitude
characteristics.text: not collected
characteristics.characteristic: biomaterial provider
characteristics.text: not collected
characteristics.characteristic: collected by
characteristics.text: 2021-08-09
characteristics.characteristic: collection date
characteristics.text: not collected
characteristics.characteristic: culture collection
characteristics.text: not collected
characteristics.characteristic: depth
characteristics.text: E. coli total RNA spiked with 3′-twister type P5 ribozyme fragment was used for strategy with specific adapter ligation to 5′-OH using PhoRtcB without tRNA blocking before. Sequencing was performed with ~50,000-100,000 reads and 150 bp paired-end.
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: env_broad_scale
characteristics.text: F- mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 deltalacX74 nupG recA1 araD139 delta(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 lambda-)
characteristics.characteristic: genotype
characteristics.text: Germany: Leipzig
characteristics.characteristic: geo loc name
characteristics.text: not collected
characteristics.characteristic: host_tissue_sampled
characteristics.text: not collected
characteristics.characteristic: identified by
characteristics.text: Library of RtcB ligation method without tRNA blocking
characteristics.characteristic: isolation source
characteristics.text: not collected
characteristics.characteristic: lab host
characteristics.text: not collected
characteristics.characteristic: lat lon
characteristics.text: not collected
characteristics.characteristic: mating type
characteristics.text: Escherichia coli K-12
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83333
characteristics.characteristic: organism
characteristics.text: not collected
characteristics.characteristic: passage_history
characteristics.text: RtcB1
characteristics.characteristic: replicate
characteristics.text: not collected
characteristics.characteristic: samp_size
characteristics.text: cell culture
characteristics.characteristic: sample type
characteristics.text: not collected
characteristics.characteristic: serotype
characteristics.text: not collected
characteristics.characteristic: serovar
characteristics.text: not collected
characteristics.characteristic: specimen voucher
characteristics.text: K-12
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002067
characteristics.characteristic: strain
characteristics.text: 37 °C
characteristics.characteristic: temp
characteristics.text: RtcB1
characteristics.characteristic: title