SAMN27024884
External Id
CGTTACTA
Fwd_Primer_Barcode
University of Wisconsin - Madison
INSDC center name
2022-03-28T00:00:00Z
INSDC first public
2022-03-31T01:32:59.120Z
INSDC last update
SRS12443381
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.5.0
NCBI submission package
TCAGCGTT
Rev_Primer_Barcode
SRS12443381
SRA accession
840 mm
annual_season_precpt
5 C
annual_season_temp
2018-08-30
collection date
hardwoods (e.g. oak,hickory,elm,aspen)
cur_land_use
northern mesic forest, early-to-mid seral
cur_vegetation
15-20 cm
http://purl.obolibrary.org/obo/UO_0000015
depth
mixed trt sample, see times_mixed column
description
moderately well
drainage_class
430 m
elev
woodland area[ENVO:00000109]
env_broad_scale
woodland area[ENVO:00000109]
env_local_scale
soil
env_material
16 week lab incubation with physical mixing treatments
extreme event
Luvisols
fao_class
USA:Wisconsin
geo loc name
45.748 N 90.731 W
lat lon
Freeon silt loam
local_class
USDA soil classification
local_class_meth
T
mixing_set
Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
nucl_acid_ext
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
pcr cond
aerobe
http://purl.obolibrary.org/obo/EO_0007024
rel_to_oxygen
4 C until sieving to 2mm and start of incubation at RT
samp mat process
Metal core, 1.8 cm dia
samp_collect_device
50 mg
samp_size
Illumina MiSeq 2x250 PE
seq meth
2mm
sieving
Freeon silt loam
soil type
USDA soil classification
soil_type_meth
after 16 week incubation, samples were stored at -80 C for up to a month prior to extraction
store_cond
16S rRNA
target gene
V4
target subfragment
4
times_mixed
241-4x
title