SAMN27484991
External Id
Guangdong Meilikang Bio-Science Ltd., China
INSDC center name
2022-04-09T00:00:00Z
INSDC first public
2022-04-09T07:37:52.473Z
INSDC last update
SRS12551334
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS12551334
SRA accession
2019-06
collection date
China: Mudong
geo loc name
Rhinogobio cylindricus
host
29.58 N 106.84 E
lat lon
gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_749906
organism
anaerobe
rel_to_oxygen
Gut microbial DNA was extracted using a PowerSoil DNA extraction kit (QIAGEN, Hilden, Germany). The V4-V5 hypervariable region of the prokaryotic 16S rDNA was amplified using primers 515F and 909R, as previously described.
samp mat process
Individuals of R. cylindricus were collected using the gill net technique. All collected individuals were measured and weighted. Fish guts were then collected and stored in plastic vials at -20 C until further analysis.
samp_collect_device
0.3 g
samp_size
SumGMMudong4
source_material_id
SumGMMudong
title