characteristics.text: SAMN23039138
characteristics.characteristic: External Id
characteristics.text: Laboratory of Genetics and Genomics, National Institute on Aging, Shuaikun Su
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-08T02:05:16.307Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS11041848
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS11041848
characteristics.characteristic: SRA accession
characteristics.text: HCT116
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002824
characteristics.characteristic: cell line
characteristics.text: Wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: polyA RNA
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000869
characteristics.characteristic: molecule
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: human colorectal carcinoma cell line
characteristics.characteristic: source_name
characteristics.text: set63.WT.3.RNA-seq.rep3
characteristics.characteristic: title
characteristics.text: Cells were treated with 100µg/mL cycloheximide for 10min and then lysed in polysome lysis buffer. After centrifugation, 100 µL cell lysates were used to extract RNA with TRIzol. Then 5~10 µg RNA was used to purify mRNAs with oligo dT beads. The purified mRNAs were used for RNA-seq library preparation.
characteristics.characteristic: treatment