SAMN29203434
External Id
Wilkes University
INSDC center name
2022-06-19T00:00:00Z
INSDC first public
2022-06-19T21:02:34.697Z
INSDC last update
SRS13488158
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS13488158
SRA accession
15weeks
age
Kalter
biomaterial_provider
WT
cell_line
Fibroblast
cell_subtype
connective tissue cell
cell_type
Valerie Kalter
collected_by
2018-01-30
collection_date
WT fibroblasts
description
adult
dev_stage
colonic tumor
disease
10 weeks
disease_stage
wild type
genotype
Briefly, freshly isolated colonic tumors (5 mm in diameter) were immediately cut into 1-2 mm3 pieces, washed with Hanks balanced salt solution (HBSS), and digested by collagenase and dispase in Dulbeccos Modified Eagle Medium (DMEM) with 1% Fetal Bovine Serum. Antibiotics were added at 37 for 3 hours with gentle shaking. The digested material was washed several times and intestinal crypts were pelleted by centrifugation. Crypts suspended in 0.5 ml of media were distributed to collagen coated tissue culture flasks. Cells were maintained at 37 in 7.5% CO2 in DMEM + 2.5% FBS with antibiotics, and after several passes fibroblast cultures were established. At 90% confluence, cultures were dissociated with 25% Trypsin (Hyclone, Rockville, MD) and distributed in centrifuge tubes of 3mL (3 million cells/ml).
growth_protocol
WT mouse with tumor
isolate
single intraperitoneal injection of 10 mg/kg of AOM, the first day before the cycles were started with DSS. Each cycle consisted of 7 days of DSS administration followed by 10 days of ordinary tap water administration. A total of four cycles were scheduled in order to simulate the cycle of acute flare-ups alternating with periods of disease inactivity observed in human IBD. Mice were sacrificed, colons were opened longitudinally, and tumors were identified and excised.
isolation_source
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
CL-0002320
phenotype
Cell culture
sample_type
male
sex
WT genes. Index32_OtC0621_Linda.Gutierrez_
specimen_voucher
Frozen in liquid nitrogen. ~3 months
store_cond
C57BL/6J
strain
Colonic tumor
tissue
WT
title
None
treatment