SAMN29328818
External Id
University Hospital Bonn
INSDC center name
2022-06-24T00:00:00Z
INSDC first public
2022-06-29T10:01:16.090Z
INSDC last update
SRS13625736
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS13625736
SRA accession
2021-02-05
collection_date
This strain was constructed by allelic exchange using the pMAD system. A 500 bp fragment of the clpP gene of S. aureus SG511 Berlin was amplified via PCR, cloned into the pMAD vector, transduced into S. aureus CmR-01, a cervimycin resistant S. aureus mutant, and allelic replacement was performed to revert the amino acid exchange encoded in CmR-01 (ClpP-I29F). The absence of the specific mutation was verified via PCR and sequencing before performing whole genome sequencing. Thus, the ClpP-I29F mutation from CmR-01 was cured (ClpP-F29I), but the WalK mutation of CmR-01 was maintained (WalK-A243V).
description
Germany: Bonn
geo_loc_name
laboratory derived
isolation_source
Staphylococcus aureus
http://purl.obolibrary.org/obo/NCBITaxon_1280
organism
bacterial culture
sample_type
WalK-A243V
strain
Staphylococcus aureus WalK-A243V
title