SAMN26577455
External Id
INRAE
INSDC center name
2022-07-01T00:00:00Z
INSDC first public
2022-07-01T09:02:02.848Z
INSDC last update
SRS12243852
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS12243852
SRA accession
missing
age
Jemalong A17
cultivar
Nodules were collected on the first 40 cm of roots below the root collar at 14 days post inoculation (dpi).
description
missing
geo_loc_name
Medicago truncatula cv Jemalong A17 seeds were germinated and transferred to aeroponic caissons under the following chamber conditions: temperature: 22C; 75% hygrometry, light intensity: 200 E m-2 s-1, light-dark photoperiod: 16-8 h. Plants were grown for 7 days in caisson growth medium (Journet, E.P., El-Gachtouli, N., Vernoud, V., et al. 2001, Medicago truncatula ENOD11: a novel RPRP-encoding early nodulin gene expressed during mycorrhization in arbuscule-containing cells, Mol. Plant Microbe Interact) supplemented with 10 mM NH4NO3, before growth in nitrogen-free medium for 3 days prior to inoculation with S. meliloti.
growth_protocol
Medicago truncatula
http://purl.obolibrary.org/obo/NCBITaxon_3880
organism
whole nodule 14dpi
tissue
M. truncatula and S. meliloti: NaCl
title
Inoculation with Sinorhizobium meliloti 2011. 150 mM NaCl was added to the medium at 13dpi and whole nodules ware harvested after 24 hours
treatment