SAMN29514069
External Id
Beckham Group, National Renewable Energy Laboratory
INSDC center name
2022-07-06T00:00:00Z
INSDC first public
2022-07-06T02:03:18.500Z
INSDC last update
SRS13715931
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS13715931
SRA accession
Adam M Deutschbauer, Lawrence Berkeley National Laboratory
biomaterial_provider
Andrew Borchert
collected_by
2021-04-13
collection_date
1 mL of a ML5 transposon library aliquot grown in LB+ 50µg/mL kanamycin to an OD600 of ~1.0 and saved at -80 ˚C was inoculated into 25 mL M9 + 20 mM D-glucose (125 mL baffled flask) and grown to an OD600 of ~1.0 (shaking 225 RPM) prior to pelleting and storing at -80 ˚C
description
Tc1 insertion library of KT2440 (pWW0- and restriction- derivative of P. putida mt-2 )
genotype
USA
geo_loc_name
M9-1C
isolate
Tc1 mutagenesis of KT2440
isolation_source
Pseudomonas putida
http://purl.obolibrary.org/obo/NCBITaxon_303
organism
1 mL
samp_size
Cell culture
sample_type
KT2440 (ML5)
strain
30 ˚C
temp
Microbe sample from Pseudomonas putida
title