Model organism or animal
BioSampleModel
2022-07-06
ENA-FIRST-PUBLIC
2022-07-06
ENA-LAST-UPDATE
SAMN25028011
External Id
pda|tdoniger
INSDC center alias
pda|tdoniger
INSDC center name
2022-07-06T11:24:54Z
INSDC first public
2022-07-06T11:24:54Z
INSDC last update
public
INSDC status
SRS11690490
SRA accession
BSF PRS TGIRT
Submitter Id
NCBI
broker name
Bloodstream form
cell_line
Bloodstream form
cell_subtype
Bloodstream form
cell_type
RNA (~800 ng) was fragmented and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), cleaned by Agencourt RNA clean XP beads (Beckman Coulter) and ligated to a 3' linker using high concentration T4 RNA Ligase 1 (NEB) in a buffer containing DMSO, ATP, PEG 8000 and RNase inhibitor (NEB). The ligated RNA was cleaned from excess linker using Dynabeads® MyOne™ SILANE beads (Thermo Scientific), and first strand cDNA was prepared using the TGIRTTM-III Enzyme (InGex). The RNA was subsequently degraded using 2 ml of 1 M NaOH and the cDNA was cleaned using Dynabeads® MyOne™ SILANE beads (Thermo Scientific). The cDNA was further ligated to a 3' adapter using high concentration T4 RNA Ligase 1 (NEB) and cleaned of excess adapter by using Dynabeads® MyOne™ SILANE beads (Thermo Scientific). The adapter ligated cDNA was PCR enriched using NEBNext® High-Fidelity (NEB) polymerase (9 PCR cycles) and sequenced in a Nextseq system (Illumina) in paired end mode (40 million reads for each sample).
description
Bloodstream form
dev_stage
T. brucei 427 BSF wild-type cells was aerobically cultivated at 37°C under 5% CO2 in HMI-9 medium supplemented with 10% fetal calf serum.
growth_protocol
T. brucei 427 BSF wild-type
isolate
Post ribosomal supernatant (PRS)
isolation_source
Trypanosoma brucei
http://purl.obolibrary.org/obo/NCBITaxon_5691
organism
Post ribosomal supernatant (PRS)
sample_type
not applicable
sex
not applicable
tissue
Trypanosoma brucei
title
No treatment
treatment