Model organism or animal
BioSampleModel
2022-04-26
ENA-FIRST-PUBLIC
2022-04-26
ENA-LAST-UPDATE
SAMN27615981
External Id
pda|lucly
INSDC center alias
pda|lucly
INSDC center name
2022-04-26T01:30:13Z
INSDC first public
2022-04-26T01:30:13Z
INSDC last update
public
INSDC status
SRS12644528
SRA accession
H001
Submitter Id
E17.5
age
NCBI
broker name
Primary cortical neuron
cell_line
Primary culture
collected_by
house mouse
common name
The brains are the major oxygen consuming organs and thus vulnerable to injury due to hypoxia or insufficient blood perfusion. Genes and metabolites alterations in brain might be adaptive to such condition and thus the neurons are most affected cell types in brain, but the molecular mechanisms lack simultaneous evidence. In the study, we employed an integrated analysis from RNA-sequencing based transcriptomics and LC-MS based metabolomics to simultaneously explore the potential altered differentially expressed genes and metabolites of primary cortical neurons under severe hypoxic condition (0.1% Oxygen) in short episodes (~2hr).
description
11 DIV- PCNs
dev_stage
Cortices
isolate
Isolated from the cortices of mouse
isolation_source
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
WT
phenotype
Total RNA
sample_type
pooled male and female
sex
C57BL6
strain
Cortices
tissue
Primary cortical neuron cultures from Mus musculus
title
0.1% Oxygen
treatment