characteristics.text: SAMN19844192
characteristics.characteristic: External Id
characteristics.text: Kuwait Institute for Scientific Research (KISR)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-07-16T18:39:19.210Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-07-16T18:39:19.210Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS9271518
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS9271518
characteristics.characteristic: SRA accession
characteristics.text: 21 d
characteristics.characteristic: age
characteristics.text: Vinod Kumar
characteristics.characteristic: biomaterial_provider
characteristics.text: Anisha Shajan
characteristics.characteristic: collected_by
characteristics.text: not applicable
characteristics.characteristic: cultivar
characteristics.text: seedling
characteristics.characteristic: dev_stage
characteristics.text: Columbia (Col-0)
characteristics.characteristic: ecotype
characteristics.text: Kuwait: KISR
characteristics.characteristic: geo_loc_name
characteristics.text: Arabidopsis seeds (Columbia-0) were surface sterilized using 70% ethanol for 2 minutes. After centrifuging the supernatant was discarded. The Arabidopsis seeds were treated with Clorox for 10 minutes. The mixture was centrifuged and the supernatant was discarded. The seeds were washed with sterile distilled water 3 times for 5 minutes. The surface-sterilized seeds were used for the experiment. The seeds were transferred to Magenta boxes containing half-strength Murashige and Skoog (MS) medium with MS vitamins, 3% sucrose, and 0.7% agar. The seeds were incubated in a growth chamber at 25 degree C temperature for 20 days. After 20 days, for each treatment five Arabidopsis seedlings were transferred to liquid media containing half-strength MS salts, MS vitamins, 1% sucrose with or without heavy metal salts. The control set was not exposed to any added heavy metals. The seedlings of the treatment sets were exposed to either 10 µM lead nitrate or 10 µM sodium orthovanadate. During the treatment, the seedlings were incubated in an incubator shaker set at 100 rpm for 24 h at 25oC with a 16/8-h light/dark cycle. RNA was isolated after 24 h from the control and heavy metal treated Arabidopsis whole seedling using RNA kit (Spectrum Plant Total RNA kit Sigma). The purity and quality of RNA were analyzed using a Nanodrop spectrophotometer and agarose gel electrophoresis prior to sequencing.
characteristics.characteristic: growth_protocol
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Whole seedling
characteristics.characteristic: tissue
characteristics.text: Arabidopsis thaliana treated with vanadium
characteristics.characteristic: title
characteristics.text: 24 h exposure to 10µM Vanadium
characteristics.characteristic: treatment