characteristics.text: GGTCGTGC
characteristics.characteristic: DeplexedBarcode
characteristics.text: SAMN24466349
characteristics.characteristic: External Id
characteristics.text: Broad Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-08T16:55:37.479Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 64
characteristics.characteristic: age
characteristics.text: Genetic Perturbation Platform
characteristics.characteristic: biomaterial provider
characteristics.text: IPC298
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006447
characteristics.characteristic: cell line
characteristics.text: Per biological replicate, cells were transduced at 750-1000X representation with the 12k Digenic Paralog CRISPR Library in cell lines stably expressing S. Pyogenese Cas9. Cells were selected with puromycin for 3 days post-transduction and passaged for 16 days after selection. Genomic DNA was collected from these cells was PCR amplified and sequenced.
characteristics.characteristic: description
characteristics.text: Skin Cancer
characteristics.characteristic: disease
characteristics.text: lentiviral integrate
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: RepA
characteristics.characteristic: rep
characteristics.text: VCR1_SCR27-RepA
characteristics.characteristic: sample
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: skin
characteristics.characteristic: tissue
characteristics.text: Human sample from Homo sapiens
characteristics.characteristic: title
characteristics.text: VCR1_SCR27
characteristics.characteristic: tracrRNA