characteristics.text: SAMN22418628
characteristics.characteristic: External Id
characteristics.text: Okinawa Institute of Science and Technology Graduate University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-07-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-07-18T19:48:03.182Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS10646711
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS10646711
characteristics.characteristic: SRA accession
characteristics.text: Yohei Yokobayashi, Yokobayashi Unit, OIST
characteristics.characteristic: biomaterial_provider
characteristics.text: 2020-10-07
characteristics.characteristic: collection_date
characteristics.text: E. coli MG1655 was cultured in LB medium supplemented with 50mM MOPS pH 7.2 and 0.2 % glucose. Overnight cultures grown at 37°C were diluted into fresh medium and grown until reaching an OD600 of about 1.0 at the target temperature. These cultures were used to inoculate 50 ml medium at the desired temperature in 500ml Erlenmeyer flasks with baffles at a target OD of 0.01. Cultivation was always performed with shaking at 250 rpm. OD was determined with a NanoDrop One in cuvette mode. The cultures (1.4 ml) were harvested by centrifugation at 21000g for 20 seconds after reaching an OD of around 0.5 (mid-exponential phase). Cells were kept growing for at least 45 doubling times (as measured in exponential phase) to reach stationary phase. Samples of 0.2 ml from the stationary phase cultures were harvested for DNA extraction. The pellets were immediately frozen at -80°C until DNA extraction. DNA was extracted in parallel using Genomic DNA Purification Kit from Thermo Fisher Scientific.
characteristics.characteristic: description
characteristics.text: Japan: Okinawa
characteristics.characteristic: geo_loc_name
characteristics.text: exponential
characteristics.characteristic: growth_phase
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: MG1655
characteristics.characteristic: isolate
characteristics.text: not applicable
characteristics.characteristic: isolation_source
characteristics.text: Escherichia coli K-12
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83333
characteristics.characteristic: organism
characteristics.text: biological replicate 2
characteristics.characteristic: replicate
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: K-12
characteristics.characteristic: strain
characteristics.text: 37
characteristics.characteristic: temp
characteristics.text: Escherichia coli str. K-12 substr. MG1655 grown at 37 °C in exponential phase, replicate 2
characteristics.characteristic: title