Plant
BioSampleModel
2016-04-01
ENA-FIRST-PUBLIC
2016-04-01
ENA-LAST-UPDATE
SAMN04590508
External Id
pda|ammorse@ufl.edu
INSDC center alias
pda|ammorse@ufl.edu
INSDC center name
2016-04-01T01:29:24Z
INSDC first public
2016-04-01T01:29:24Z
INSDC last update
public
INSDC status
SRS1368030
SRA accession
CCC
Submitter Id
1 month before flowering
age
Koen J.F. Verhoeven Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Netherlands
biomaterial_provider
NCBI
broker name
Koen J.F. Verhoeven
collected_by
Parental lineage treatments: Control treatments were applied to the upper surface of 3-4 fully expanded leaves. In generation 1, 0.75 ml of solution per plant was applied to 8-week old plants; in generation 2, a total amount of 0.75 ml was applied to each plant distributed over two application moments when plants were 5 and 7 weeks old. In both generations treatment application was during vegetative growth, approximately 1 month prior to first flowering. Offspring treatments: Seeds from a single seed head were weighed individually, surface-sterilized and germinated on 0.8% agar plates. After 10 days, seedlings were transplanted to individual pots and grown under climate chamber conditions in fully randomized blocks. Each block contained two plants per parental lineage; one received a JA treatment and the other a control treatment. Control-treated plants received 0.25 ml of an ethanol/Triton-X solution. 3h after control application two standardized leaves younger than the JA-treated leaves were collected, discarding the latex-rich mid-vein, flash-frozen in liquid nitrogen and stored at -80C for RNA analysis.
description
Netherlands: near Heteren
geo_loc_name
14h light / 10h dark 20C / 15C
growth_protocol
A68
isolate
Taraxacum officinale
http://purl.obolibrary.org/obo/NCBITaxon_50225
organism
leaf
tissue
Taraxacum officinale
title
control parental treatment / control offspring treatment
treatment