2022-07-11
ENA first public
2022-07-11
ENA last update
ERC000011
ENA-CHECKLIST
SAMEA110234654
External Id
Heinrich-Heine University Duesseldorf
INSDC center alias
Heinrich-Heine University Duesseldorf
INSDC center name
2022-07-11T00:21:03Z
INSDC first public
2022-07-11T00:21:03Z
INSDC last update
public
INSDC status
ERS12332280
SRA accession
E-MTAB-11901:Sample 12
Submitter Id
ArrayExpress
broker name
baker's yeast
common name
Protocols: After Sasidharan et al. 2012 (doi: 10.1002/yea.2911) Culture samples were immediately quenched in ethanol and disrupted using acid-washed zir- conia/silica beads (0.5 mm; Tomy Seiko Co., Ltd., Japan) with sodium acetate buffer (250 µL; sodium acetate 300 mM, Na2-EDTA 10 mM , pH 4.5–5.0) and one volume of TE-saturated phenol (Nacalai Tesque) equilibrated with sodium acetate buffer (250 µL). The samples were then centrifuged (12 000 g, 15 min, 4 ◦ C) and the aqueous phase transferred to fresh 1.5 mL microcentrifuge tubes. Back-extraction was performed by adding sodium acetate buffer (125 µL) to the bead-beat tubes, vortex (10 s), centrifuging (12 000 g, 15 min, 4 ◦ C) and adding the aqueous phase to the ﬁrst aqueous phase. 2.5 volumes ice-cold 99.5 % ethanol were added to the aqueous phase and RNA/DNA precipitated at −20 ◦ C overnight. The samples were then centrifuged (12 000 g, 30 min, 4 ◦ C), the supernatant removed by aspiration, and pellets washed 3× in 500 µL 70 % ethanol and air-dried (10 min, room temperature). DNA was removed (RNase-Free DNase Set; Qia- gen, Japan) and RNA recovered by column puriﬁcation (QIAquick PCR Puriﬁcation Kit; Qiagen, Japan) in 50 µL UltraPure water, and stored at −80 ◦ C prior to analysis. dUTP method (doi: 10.1093/nar/gkp596 and doi: 10.1038/nmeth.1491)
description
not applicable
isolate
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Saccharomyces cerevisiae
organism
IFO 0233
strain
Sample 12
title