2022-07-11
ENA first public
2022-07-11
ENA last update
ERC000011
ENA-CHECKLIST
SAMEA110284988
External Id
Wellcome Sanger Institute
INSDC center alias
Wellcome Sanger Institute
INSDC center name
2022-07-11T04:18:45Z
INSDC first public
2022-07-11T04:18:45Z
INSDC last update
public
INSDC status
ERS12382598
SRA accession
E-MTAB-9543_3:Human_colon_16S8117830
Submitter Id
70 to 75
year
age
ArrayExpress
broker name
unsorted
cell type
human
common name
Protocols: For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. cDNA library preparation was were carried out according to the manufacturer’s protocol.
description
adult
developmental stage
386C
individual
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Homo sapiens
organism
transverse colon
organism part
female
sex
Human_colon_16S8117830
title