ERC000013
ENA-CHECKLIST
2022-07-09
ENA-FIRST-PUBLIC
2022-07-09
ENA-LAST-UPDATE
SAMEA7050491
External Id
QUEEN'S UNIVERSITY BELFAST
INSDC center alias
QUEEN'S UNIVERSITY BELFAST
INSDC center name
2022-07-09T00:20:14Z
INSDC first public
2022-07-09T00:20:14Z
INSDC last update
public
INSDC status
ERS4811121
SRA accession
Positive control
Submitter Id
Illumina adapters
adapters
2020
collection date
Positive control
common name
Positive Zymo control
description
lab
environment (biome)
liquid
environment (feature)
Commercial mock community
environment (material)
United Kingdom
geographic location (country and/or sea)
54.579.058
DD
geographic location (latitude)
-5.936.998
DD
geographic location (longitude)
Belfast
geographic location (region and locality)
0
centuries
host age
host-associated
host-associated environmental package
bacteria_archaea
investigation type
PCR
nucleic acid amplification
Fast DNA SPIN Kit for Soil
nucleic acid extraction
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
95�C for 3 minutes, 20 cycles at 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds and then 72�C for 5 minutes.
pcr conditions
515F and 806R
pcr primers
EasyCare_2018
project name
-80
°C
sample storage temperature
75uL
g
sample volume or weight for DNA extraction
Illumina MiSeq
sequencing method
16S: V4- 250 bp
target gene
Control
title