2022-07-09
ENA first public
2022-07-09
ENA last update
ERC000011
ENA-CHECKLIST
SAMEA110195562
External Id
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center alias
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
2022-07-09T00:17:14Z
INSDC first public
2022-07-09T00:17:14Z
INSDC last update
public
INSDC status
ERS12293237
SRA accession
E-MTAB-11895:6_Aire_plus_plus_4_WC
Submitter Id
28
day
age
ArrayExpress
broker name
Sertoli cell
cell type
house mouse
common name
Protocols: Sertoli cells were FACS-sorted directly to the RLT lysis buffer. Teste from 21, 28 and 70 days old wild-type males were disected and decapsulated. Seminiferous tubules were disrupted mechanically using a gentle shear force and enzymatically. Obtained single cell suspension was used for staining of Sertoli cells and FACS-sorting. RNA was extracted using a RNeasy180 Plus Micro Kit (Qiagen) according to the manufacturer's protocol. Sequencing libraries were prepared using Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (Takara) according to the manufacturer's protocol.
description
pubescent
developmental stage
normal
disease
wild type genotype
genotype
6
individual
not applicable
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Mus musculus
organism
testis
organism part
male
sex
C57BL/6J
strain
6_Aire_plus_plus_4_WC
title