ERC000013
ENA-CHECKLIST
2022-07-09
ENA-FIRST-PUBLIC
2022-07-09
ENA-LAST-UPDATE
SAMEA7050503
External Id
QUEEN'S UNIVERSITY BELFAST
INSDC center alias
QUEEN'S UNIVERSITY BELFAST
INSDC center name
2022-07-09T00:20:14Z
INSDC first public
2022-07-09T00:20:14Z
INSDC last update
public
INSDC status
ERS4811133
SRA accession
InVitro_A1_O
Submitter Id
Illumina adapters
adapters
2019
collection date
InVitro_A1_O
common name
Animal 1 O
description
Rumen
environment (biome)
GIT
environment (feature)
Rumen fluid
environment (material)
United Kingdom
geographic location (country and/or sea)
54.579.058
DD
geographic location (latitude)
-5.936.998
DD
geographic location (longitude)
Belfast
geographic location (region and locality)
6 months
centuries
host age
White
host color
Sheep
host common name
O
host diet
neuter
host sex
host-associated
host-associated environmental package
bacteria_archaea
investigation type
PCR
nucleic acid amplification
Fast DNA SPIN Kit for Soil
nucleic acid extraction
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
95�C for 3 minutes, 20 cycles at 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds and then 72�C for 5 minutes.
pcr conditions
515F and 806R
pcr primers
InVitro_2019
project name
-80
°C
sample storage temperature
0.5ml
g
sample volume or weight for DNA extraction
Illumina MiSeq
sequencing method
16S: V4- 250 bp
target gene
InVitro
title