ERC000011
ENA-CHECKLIST
2022-07-10
ENA-FIRST-PUBLIC
2022-07-10
ENA-LAST-UPDATE
SAMEA9425797
External Id
KING'S COLLEGE LONDON
INSDC center alias
KING'S COLLEGE LONDON
INSDC center name
2022-07-10T00:16:22Z
INSDC first public
2022-07-10T00:16:22Z
INSDC last update
public
INSDC status
ERS7150681
SRA accession
E-MTAB-10756:Sample 1
Submitter Id
ArrayExpress
broker name
CD4-positive, CD25-positive, alpha-beta regulatory T cell
cell_type
human
common name
Protocols: Cryopreserved PBMC samples (10^6 cells) from baseline (1 or 2 weeks before first treatment) and 24 weeks post treatment timepoint were thawed from 10 subjects and stained first using live/dead violet then surface stained using CD14 CD3 CD4 CD25 CD127 antibodies. A minimum of 40,000 CD14- CD3+ CD4+ CD25+ CD127lo Tregs (average = 81,600, max = 158,300) were sorted using BD FACSAria III and frozen at -80C in minimal PBS prior to DNA/RNA extraction. DNA/RNA was extracted in parallel with the AllPrep micro kit (Qiagen) RNAseq libraries prepared from 2/3 of extracted RNA (91-140 ng) with SMART-Seq v4 and NuGen Ovation Ultralow
description
adult
developmental stage
autoimmune type 1 diabetes
disease
p6
individual
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Homo sapiens
organism
blood
organism part
female
sex
Sample 1
title