0
Broadleaf_Seedling_Dens_m2
133.44
CEC_cmol_per_kg
0.982142857
Conifer_Seedling_Dens_m2
1.16
EC_mS_per_cm
Treed bog
Ecosite_Name
Sb/labrador tea.cloudberry/peat moss
Ecosite_Phase
11818.7
Exch_Ca_mg_per_kg
253.665
Exch_K_mg_per_kg
1357.75
Exch_Mg_mg_per_kg
140.347
Exch_Na_mg_per_kg
SAMN29636310
External Id
University of Wisconsin-Madison
INSDC center name
2022-07-11T00:00:00Z
INSDC first public
2022-08-02T00:21:03.093Z
INSDC last update
SRS14388156
INSDC secondary accession
live
INSDC status
Short
Interval
Wetland
Land_Class
LARILAR
Leading_Species
7
Moisture_Numeric
Hygric
Moisture_Regime
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.5.0
NCBI submission package
1980
Old_Year
100
PCt_Conifer_Seedlings
24
Pair_ID
15
Recent_Interval
1995
Recent_Year
SRS14388156
SRA accession
16-NT-24s
Site_ID
O
Soil_layer
21
TSLF_At_Sample
42.9
Total_C_pct
2.09
Total_N_pct
0.359
Total_S_pct
0.982142857
Total_Seedling_Dens_m2
314
annual_season_precpt
-3.5
annual_season_temp
2016
collection_date
swamp (permanent or semi-permanent water body dominated by woody plants)
cur_land_use
Treed bog
cur_vegetation
Beckingham, J. D., and J. H. Archibald. 1996. Fieldguide to ecosites of Northern Alberta. NaturalResources Canada, Canadian Forest Service,Northern Forestry Centre, Edmonton, Alberta,Canada.
cur_vegetation_meth
0-0.1 m
depth
Keywords: GSC:MIxS;MIMS:5.0
description
229 m
elev
subpolar coniferous forest biome [ENVO:01000250]
env_broad_scale
soil [ENVO:00001998]
env_local_scale
forest soil [ENVO:00002261] | burned soil [ENVO:00005760]
env_medium
yes
fire
Canada: Northwest Territories
geo_loc_name
O horizon
horizon
subjective
horizon_meth
61.96788 N 118.447877 W
lat_lon
Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
nucl_acid_ext
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 69
pcr_cond
FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
pcr_primers
6.45
ph
1:5 soil dry mass to DI water
ph_meth
Metal core, 5.5 cm i.d.
samp_collect_device
Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
samp_mat_process
250 mg
samp_size
Illumina MiSeq 2x250 PE
seq_meth
16S rRNA
target_gene
V4
target_subfragment
WB16-24-S-O
title