0
Broadleaf_Seedling_Dens_m2
9.41
CEC_cmol_per_kg
11.9
Conifer_Seedling_Dens_m2
0.106
EC_mS_per_cm
Lichen
Ecosite_Name
Pj/bearberry/lichen
Ecosite_Phase
857.379
Exch_Ca_mg_per_kg
73.566
Exch_K_mg_per_kg
89.026
Exch_Mg_mg_per_kg
12.216
Exch_Na_mg_per_kg
SAMN29636294
External Id
University of Wisconsin-Madison
INSDC center name
2022-07-11T00:00:00Z
INSDC first public
2022-08-02T00:20:54.537Z
INSDC last update
SRS14388111
INSDC secondary accession
live
INSDC status
Long
Interval
Upland
Land_Class
PINUBAN
Leading_Species
3
Moisture_Numeric
Subxeric
Moisture_Regime
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.5.0
NCBI submission package
1976
Old_Year
100
PCt_Conifer_Seedlings
19
Pair_ID
39
Recent_Interval
2015
Recent_Year
SRS14388111
SRA accession
16-NT-19l
Site_ID
O
Soil_layer
1
TSLF_At_Sample
4.75
Total_C_pct
0.159
Total_N_pct
0.022
Total_S_pct
11.9
Total_Seedling_Dens_m2
296
annual_season_precpt
-5
annual_season_temp
2016
collection_date
conifers (e.g. pine,spruce,fir,cypress)
cur_land_use
Lichen
cur_vegetation
Beckingham, J. D., and J. H. Archibald. 1996. Fieldguide to ecosites of Northern Alberta. NaturalResources Canada, Canadian Forest Service,Northern Forestry Centre, Edmonton, Alberta,Canada.
cur_vegetation_meth
0-0.0217 m
depth
Keywords: GSC:MIxS;MIMS:5.0
description
240 m
elev
subpolar coniferous forest biome [ENVO:01000250]
env_broad_scale
soil [ENVO:00001998]
env_local_scale
forest soil [ENVO:00002261] | burned soil [ENVO:00005760]
env_medium
yes
fire
Canada: Northwest Territories
geo_loc_name
O horizon
horizon
subjective
horizon_meth
62.499045 N 113.414147 W
lat_lon
Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
nucl_acid_ext
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 53
pcr_cond
FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
pcr_primers
5.54
ph
1:5 soil dry mass to DI water
ph_meth
Metal core, 5.5 cm i.d.
samp_collect_device
Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
samp_mat_process
250 mg
samp_size
Illumina MiSeq 2x250 PE
seq_meth
16S rRNA
target_gene
V4
target_subfragment
WB16-19-L-O
title