SAMN29636356
External Id
University of Wisconsin-Madison
INSDC center name
2022-08-01T00:00:00Z
INSDC first public
2022-08-02T00:21:07.963Z
INSDC last update
SRS14388179
INSDC secondary accession
live
INSDC status
MIMS.me
NCBI submission model
MIGS/MIMS/MIMARKS.soil
NCBI submission model
MIMS.me.soil.5.0
NCBI submission package
SRS14388179
SRA accession
Blank26
Site_ID
Keywords: GSC:MIxS;MIMS:5.0
description
Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
nucl_acid_ext
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 115
pcr_cond
FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
pcr_primers
Illumina MiSeq 2x250 PE
seq_meth
16S rRNA
target_gene
V4
target_subfragment
WB16-B26
title