ERC000011
ENA-CHECKLIST
2022-08-02
ENA-FIRST-PUBLIC
2022-08-02
ENA-LAST-UPDATE
SAMEA8135618
External Id
KAROLINSKA INSTITUTE
INSDC center alias
KAROLINSKA INSTITUTE
INSDC center name
2022-08-02T00:25:24Z
INSDC first public
2022-08-02T00:25:24Z
INSDC last update
public
INSDC status
ERS5822440
SRA accession
E-MTAB-10096:D3_33
Submitter Id
7
day
age
ArrayExpress
broker name
human
common name
Protocols: Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife). Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst in media supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol. the cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies).
description
embryo stage
developmental stage
Treat
growth condition
D3
individual
mural
inferred lineage
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Homo sapiens
organism
whole organism
organism part
female
sex
D3_33
title