SAMN19332828
External Id
Anatomy and Cell Biology, Kyoto University, Graduate school of medicine
INSDC center name
2022-08-05T00:00:00Z
INSDC first public
2022-08-05T01:28:16.183Z
INSDC last update
SRS9057725
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS9057725
SRA accession
hiPSCs were maintained under a feeder-free condition [StemFitTM (Ajinomoto, Tokyo, Japan) medium on recombinant LAMININ511 (rLN511E8) (iMatrix-511, Nippi, Tokyo, Japan)-coated cell culture plates] (Nakagawa et al., 2014). The hPGCLCs were induced by plating 5.0 × 10^3 iMeLCs into a well of a low-cell-binding V-bottom 96-well plate (greiner, 651970) in GK15 supplemented with 200 ng/ml of BMP4, 100 ng/ml of SCF (R&D Systems, 455-MC), 50 ng/ml of EGF (R&D Systems, 236-EG), 1000 U/ml of LIF (Millipore, #LIF1005) and 10 µM of the ROCK inhibitor.
cell culture protocol
585B1-868
cell line
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
human primodial germ cell like cell (PGCLC)
source cell type
hiPS and d4 BTAG+
source_name
mix of hiPSC and day4 hPGCLC [BTAG+]
title