Metagenome or environmental
BioSampleModel
2022-08-11
ENA-FIRST-PUBLIC
2022-08-11
ENA-LAST-UPDATE
SAMN30111230
External Id
pda|ekwear
INSDC center alias
pda|ekwear
INSDC center name
2022-08-11T03:21:51Z
INSDC first public
2022-08-11T03:21:51Z
INSDC last update
public
INSDC status
SRS14421641
SRA accession
ColumbiaR_BB_22Feb22
Submitter Id
NCBI
broker name
2022-02-23
collection_date
Caveat: the sequencing method produced an abnormal number of chimeras; screening will be especially important with these samples.
description
USA: Oregon, Portland, Columbia River at Broughton Beach Park
geo_loc_name
river water
isolation_source
45.601110 N 122.616365 W
lat_lon
freshwater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_449393
organism
aerobe
rel_to_oxygen
Collected by dunking a bottle on a sampling pole from shore
samp_collect_device
Water samples were returned to the lab at ~ ambient temperature. Duplicate whole-water samples were collected on 0.2 µm pore size, 47 mm diameter filters (Millipore GSWP mixed cellulose ester) using low vacuum pressure from a manual pump. Filters were stored frozen (-20 degC) until DNA was extracted using a ZymoBIOMICS DNA Miniprep Kit. 16S rRNA gene amplicons were generated from combined duplicate samples using primers 515F-Y and 926R. Amplicons were gel-extracted targeting bands at 400-700 bp using a ZymoClean Gel Recovery Kit. Amplicons were sequenced by Azenta US Inc.
samp_mat_process
0.43 and 0.46 L
samp_size
Columbia River at Broughton Beach Park
title