SAMN30312313
External Id
Doug Higgs, Weatherall Institute of Molecular Medicine, University of Oxford
INSDC center name
2022-08-18T00:50:19.110Z
INSDC first public
2022-08-18T00:50:19.110Z
INSDC last update
SRS14649175
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS14649175
SRA accession
Ter119+ erythroid cells purified from the spleen of a INVEN Phenylhydrazine APH-treated mice, sample 2
cell type
Primary erythroid cells derived from spleen from INVEN APH-treated mice are disaggregated and cells stained with ter119-PE antibody (BD Biosciences at a working concentration of 2ug/ml) then incubated with anti-PE MACS beads (Miltenyi) and purified using LS columns (Miltenyi), all following manufacturor's instructions.
cell type
H3K27me3 Cell Signaling 9733 2ug/ml
chip antibody
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
mouse derived Erythroid cells
source_name
C57Bl8
strain
APHSpleen_INVEN_Ter119_H3K27me3_3
title
Genome-wide
treatment