ERC000011
ENA-CHECKLIST
2022-08-21
ENA-FIRST-PUBLIC
2022-08-21
ENA-LAST-UPDATE
SAMEA9926424
External Id
Department of Biology - Lund University
INSDC center alias
Department of Biology - Lund University
INSDC center name
2022-08-21T00:12:24Z
INSDC first public
2022-08-21T00:12:24Z
INSDC last update
public
INSDC status
ERS7604772
SRA accession
E-MTAB-10920:Sample 5
Submitter Id
12
day
age
ArrayExpress
broker name
thale cress
common name
Protocols: For untreated samples (time point 0 minutes), approximately 12 seedlings were gently lifted from the MS agar plate, placed inside a microcentrifuge tube and snap frozen immediately in liquid nitrogen. Arabidopsis seeds were surface sterilized with chlorine gas. The seeds were sown in plates containing 1x MS with vitamins, 1% sucrose, 1% phytoagar, 0.5 g/L MES and pH adjusted to 5.8. The plates were stratified at 4°C for 3 days before transferring to standard growth condition (21°C, 16-h light/8-h dark cycle, 100 µmol m-2s-1). Data were processed using Demultiplexing and quality controlled with FastQC. RNA was extracted using the Sigma Spectrum Plant kit and DNAse treatment. 500 ng of total RNA was used for library preparation using Illumina TruSeq mRNA (poly-A selection) and TruSeq RNA UD Indexes for up to 96 samples (Illumina, 20022371).
description
seedling development stage
developmental stage
Col-0
ecotype
wild type genotype
genotype
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
Arabidopsis thaliana
organism
whole organism
organism part
22
minute
sampling time point
brushing
stimulus
Sample 5
title