ERC000011
ENA-CHECKLIST
2022-08-19
ENA-FIRST-PUBLIC
2022-08-19
ENA-LAST-UPDATE
SAMEA110455453
External Id
ETH Zurich, DBSSE
INSDC center alias
ETH Zurich, DBSSE
INSDC center name
2022-08-19T00:14:15Z
INSDC first public
2022-08-19T00:14:15Z
INSDC last update
public
INSDC status
ERS12553483
SRA accession
E-MTAB-11997:GLI3_KO_D1_45d
Submitter Id
ArrayExpress
broker name
human
common name
Protocols: Single cell suspensions were acquired by dissociation of the organoids with a papain-based dissociation (Miltenyi Biotec, 130-092-628). Cerebral organoids were generated using a whole brain organoid differentiation protocol (Lancaster et al. 2013). Single cell RNAseq libraries were generated with the Chromium Single Cell 3’ V3 Library & Gel Bead Kit. Single cell encapsulation and library preparation were performed according to the manufacturer's protocol. Single cell RNAseq libraries were generated with the Chromium Single Cell 3’ V3 Library & Gel Bead Kit. Single cell encapsulation and library preparation were performed according to the manufacturer's protocol.
description
GLI3 knockout
genotype
GLI3 KO 1
line
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Homo sapiens
organism
cerebral organoid
organism part
1
replicate
45
day
time
GLI3_KO_D1_45d
title