ERC000011
ENA-CHECKLIST
2022-08-22
ENA-FIRST-PUBLIC
2022-08-22
ENA-LAST-UPDATE
SAMEA110682531
External Id
School of Life Sciences University of Warwick UK
INSDC center alias
School of Life Sciences University of Warwick UK
INSDC center name
2022-08-22T16:14:40Z
INSDC first public
2022-08-22T16:14:40Z
INSDC last update
public
INSDC status
ERS12780098
SRA accession
E-MTAB-12114:WT3
Submitter Id
ArrayExpress
broker name
Protocols: Early stationary (10h) planktonic cultures grown in BHI-S media in anaerobic conditions. 5 mL of culture was resuspended in 5 mL ice-cold LETS buffer. Cells were lysed using Fast-prep 24 instrument (MP Bioscience). Trizol (Ambicon, USA) was used for RNA extraction in phenol-chloroform extraction. Total RNA was treated with DNase, rRNA removal, cDNA reverse transcription and library preparation. rRNA removal, cDNA reverse transcription and library preparation was conducted by Novogene (China).
description
early stationary phase
developmental stage
wild type genotype
genotype
BHI-S media in anaerobic conditions
growth condition
Clostridioides difficile
http://purl.obolibrary.org/obo/NCBITaxon_1496
organism
Clostridioides difficile
organism
whole organism
organism part
R20291
strain
WT3
title