Metagenome or environmental
BioSampleModel
2022-08-25
ENA-FIRST-PUBLIC
2022-08-25
ENA-LAST-UPDATE
SAMN30454324
External Id
pda|fabiennereiss@orcid
INSDC center alias
pda|fabiennereiss@orcid
INSDC center name
2022-08-25T01:32:34Z
INSDC first public
2022-08-25T01:32:34Z
INSDC last update
public
INSDC status
SRS14766718
SRA accession
T-I4
Submitter Id
NCBI
broker name
2020-10-22
collection_date
Soil samples were incubated with single biocides, biocide combinations or water for 28 days at room temperature. 100 mM BrdU (B5002, Merck, Darmstadt, Germany) solution were added from day 26 for 48 h to the end of incubation. According to manufacturers' instructions, DNA was extracted from all microcosms using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research Europe GmbH, Freiburg im Breisgau, Germany). We refer to this DNA as total DNA. BrdU-labeled DNA was isolated from the total DNA by an immunocapture approach described earlier (Hanson C. and McMahon S., 2006; McMahon et al., 2011). The obtained immocaptured DNA is now referred as active DNA isolated from metabolically microbial active cells.
description
Germany: Schmallenberg
geo_loc_name
soil
isolation_source
51.09 N 8.18 E
lat_lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
RefeSol-02A
ref_biomaterial
aerobe
rel_to_oxygen
Zymo Research Quick-DNA Fecal/Soil Microbe Miniprep Kit
samp_mat_process
250 microg Soil
samp_size
Soil metagenome- total isoproturon 4
title