MIGS.eu
BioSampleModel
2022-08-26
ENA-FIRST-PUBLIC
2022-08-26
ENA-LAST-UPDATE
SAMN30480170
External Id
pda|victoria_bonnell@orcid
INSDC center alias
pda|victoria_bonnell@orcid
INSDC center name
2022-08-26T01:23:43Z
INSDC first public
2022-08-26T01:23:43Z
INSDC last update
public
INSDC status
SRS14779694
SRA accession
AP2-LT+3xHA_cloneB10
Submitter Id
NCBI
broker name
2018-12-21
collection_date
malaria parasite P. falciparum
common name
This parasite line was generated using CRISPR-Cas9 and double homologous recombination to insert a 3xHA epitope tag to the 3`-end of the pfap2-lt (PF3D7_0802100) gene from Plasmodium falciparum (wild-type 3D7 background). The parasite line was cloned out using limited dilution cloning and taken off of drug selection (2.5nM WR99210) after verification of gene modification by PCR. The sample for whole genome sequencing (WGS) was prepared by lysing asynchronous parasites with 0.1% saponin for 10 minutes at room temperature followed by washes with 1xPBS to remove lysed erythrocyte material. Genomic DNA was then purified using the Qiagen DNeasy Blood and Tissue kit with no modifications and sequenced on the Illumina HiSeq 2500 using single-end adaptors. This modified line was used to identify the genome-wide binding sites of the sequence-specific transcription factor AP2-LT, using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). The ChIP-seq experiments were carried out on synchronized asexual blood stage parasites at 40hpi (hours post invasion) [schizont stage].
description
USA: Pennsylvania
geo_loc_name
Parent line was purchased from MR4 (NIAID) and grown under normal Plasmodium falciparum culturing conditions (5% O2, 7% CO2, 37C, RPMI 1640 media with 0.5% AlbumaxII and hypoaxanthine)
isol_growth_condt
Plasmodium falciparum
http://purl.obolibrary.org/obo/NCBITaxon_5833
organism
Haploid
ploidy
3D7 strain
strain
Plasmodium falciparum
title