Plant
BioSampleModel
2022-08-31
ENA-FIRST-PUBLIC
2022-08-31
ENA-LAST-UPDATE
SAMN30502409
External Id
pda|wingfly1234
INSDC center alias
pda|wingfly1234
INSDC center name
2022-08-31T01:47:31Z
INSDC first public
2022-08-31T01:47:31Z
INSDC last update
public
INSDC status
SRS14783407
SRA accession
HS 9b-1 rep 1
Submitter Id
7-days-old
age
NCBI
broker name
thale cress
common name
Arabidopsis seeds of Columbia-0 (Col-0) and alkbh9b-1 were surface sterilized in 75% ethanol for 15 min, washed with 100% ethanol for 1 min, and planted on half-strength MS media (including 1% sucrose). Prior to germination, seeds were stratified for 2 days at 4 °C under the dark condition and moved to growth chamber. For the heat stress treatment, plants were grown for 6 days at 22 °C (12 h light/12 h dark cycle) and then treated with heat stress of 37 °C for 24 h. Then were collected for both RNA-seq and Nanopore Direct RNA sequencing (DRS). The stress granule enrichment method was same as previous studies (Kim et al., Nature Plants, 2021; Lei et al., Bio-protocol, 2021). RNA-seq was carried out as standard methods. DRS library was prepared using direct RNA sequencing kit (Nanopore, SQK-RNA002), loaded onto an R9.4 Flow Cell (Flow cell type FLO-MIN106) and sequenced on a GridION device.
description
Col-0
ecotype
alkbh9b-1
genotype
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
biological replicate 1
replicate
seedlings
tissue
HS 9b-1 rep 1
title