SAMN29611982
External Id
Cornell University
INSDC center name
2022-09-02T00:00:00Z
INSDC first public
2022-09-02T10:57:03.084Z
INSDC last update
SRS13847899
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS13847899
SRA accession
2021-02-08
collection_date
WA 38
cultivar
Actively growing roots from tissue culture
dev_stage
USA: Washington State University Sunrise Research Orchard near Rock Island, WA
geo_loc_name
Research orchard in North Central Washington, USA (Shrub Steppe - Cold winters, hot arid summers), managed and irrigated according to commercial standards to maximize fruit quality
isolation_source
Malus domestica
http://purl.obolibrary.org/obo/NCBITaxon_3750
organism
Young shoots were collected from 5 apple trees, sterilized, cultured, and propagated in shoot media (Full strength MS with 10mL/L KAO & Michuyluk nutrient solution, 1mL/L Myo-Inositol, 30g/L sucorse, 1mg/L 6-Benzylaminopurine, 3mg/L Indole-3-butyric acid, 0.2mg/L Gibberellic Acid) for up to 2 years. Shoots from tissue culture were collected and cultured in root induction media (half strength MS with 0.1g/L Thiamine-HCl, 20g/L sucorse, 2.5mg/L Indole-3-butyric acid) in the dark for 7-14 days before moved to root enlogation media (same as root induction media except no Indole-3-butyric acid was added). Roots from 10 cultures were collected and pooled.
sample_type
Root
tissue
0023C_Roots_from_tissue_culture_Apple_WA38
title