SAMN23788909
External Id
Plant tissues were crushed first with a mortar and pestle and then with beads in a mixer mill MM 400 (Retsch). RNA were extracted using the RNA Nucleospin Plus kit (Macherey-Nagel), treated with rDNase (Macherey-Nagel) for 10 min at 37°C and then cleaned up with the Nucleospin RNA clean-up kit from Macherey-Nagel.
Extract_protocol
Australia 1
Genotype
M. pudica were grown under N-free conditions in Gibson tubes containing a Fahraeus slant agar and liquid Jensen 1/4th medium. Plants were incubated at 28°C under a 16-hour daylight period. Roots were kept in dark. Water was added every 2 days.
Growth_protocol
Laboratoire des Interactions Plantes-Microorganismes
INSDC center name
2022-09-03T08:10:41.753Z
INSDC first public
2022-09-03T08:10:41.753Z
INSDC last update
SRS11256289
INSDC secondary accession
live
INSDC status
polyA_RNA
Molecule
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Ralstonia solanacearum RCM1865 GMI1000 pralta hrpG efpRE66K taxid=305
Other_organism
SRS11256289
SRA accession
MpRs-hrpGefpR_10dpi_nod_R1
Title
nodules. 10 dpi. Seeds produced at LIPM in 2017 from 22 plants originated from the commercial lot Australia 1.
description
Mimosa pudica
http://purl.obolibrary.org/obo/NCBITaxon_76306
organism
nodules
tissue
MpRs-hrpGefpR_10dpi_nod.R3
title