SAMN23788931
External Id
Plant tissues were crushed first with a mortar and pestle and then with beads in a mixer mill MM 400 (Retsch). Cells were lysed in CTAB buffer (2% CTAB, 0.1 M Tris-HCl, 1.4 M NaCl, 20 mM EDTA, 2% PVPP) supplemented with 2% β-mercaptoethanol, 1% Nucleoguard (Excilone) and 0.2% ProtectRNA (Sigma) for 10 min at 65°C. RNA were subsequently extracted in three steps with chloroform:isoamyl alcohol (24:1), phenol:chloroform (5:1) and chloroform:isoamyl alcohol (24:1). RNA were precipitated with LiCl overnight at -20°C. Pellets were resuspended in RNAse-free water, then treated with DNAse (Qiagen) for 10 min at room temperature and cleaned with the RNeasy micro kit (Qiagen).
Extract_protocol
Australia 1
Genotype
M. pudica were grown under N-free conditions in Gibson tubes containing a Fahraeus slant agar and liquid Jensen 1/4th medium. Plants were incubated at 28°C under a 16-hour daylight period. Roots were kept in dark. Water was added every 2 days.
Growth_protocol
Laboratoire des Interactions Plantes-Microorganismes
INSDC center name
2022-09-02T12:05:46.857Z
INSDC first public
2022-09-02T12:05:46.857Z
INSDC last update
SRS11256311
INSDC secondary accession
live
INSDC status
polyA_RNA
Molecule
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Ralstonia solanacearum CBM125 GMI1000 pRalta hrcV taxid=305
Other_organism
SRS11256311
SRA accession
MpRs-hrcV_5dpi_bumps
Title
bumps. 5dpi. Seeds produced at LIPM in 2017 from 22 plants originated from the commercial lot Australia 1.
description
Mimosa pudica
http://purl.obolibrary.org/obo/NCBITaxon_76306
organism
bumps
tissue
MpRs-hrcV_5dpi_bumps.R1
title