Model organism or animal
BioSampleModel
2022-09-14
ENA-FIRST-PUBLIC
2022-09-14
ENA-LAST-UPDATE
SAMN30827682
External Id
pda|sarahbreedon@cmail.carleton.ca
INSDC center alias
pda|sarahbreedon@cmail.carleton.ca
INSDC center name
2022-09-14T01:44:14Z
INSDC first public
2022-09-14T01:44:14Z
INSDC last update
public
INSDC status
SRS15094859
SRA accession
CSTHRTE3
Submitter Id
Adult
age
NCBI
broker name
Couch's spadefoot toad
common name
High quality total RNA samples were sent to the Center for Applied Genomics at the SickKids Hospital, Toronto (ON, Canada) for Small RNA RNA-Seq analysis. To ensure high-quality RNA was used for library construction of the miRNA library, an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) was used to measure RNA concentration, quality, and integrity. Small cDNA libraries were constructed, and quality was then assessed on the Agilent Bioanalyzer 2100 system. Sequencing was carried out using an Illumina HiSeq 2500 platform.
design_description
Arizona
ecotype
CSTHRTE3.fastq.gz
filename
fastq
filetype
Illumina HiSeq 2500
instrument_model
CSTHRTE3
library_id
single
library_layout
size fractionation
library_selection
TRANSCRIPTOMIC
library_source
miRNA-Seq
library_strategy
Scaphiopus couchii
http://purl.obolibrary.org/obo/NCBITaxon_85089
organism
ILLUMINA
platform
male
sex
Heart
tissue
Couch spadefoot toad Heart Estivating 3
title
Scaphiopus couchii
title