SAMN28232270
External Id
Biomedicine, University of Turku / Medicity
INSDC center name
2022-09-16T00:00:00Z
INSDC first public
2022-09-16T02:08:51.980Z
INSDC last update
SRS12984580
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS12984580
SRA accession
MxCre+
genotype
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
in vitro cultured alveolar macrophages adult
source_name
Tg(Mx1-cre)1Cgn/J
strain
C57BL/6
strain background
bronchoalveolar lavage
tissue
ATACseq adult alveolar macrophages in vitro MxCre IFNa rep1 [AM_MxCre_IFNa_1]
title
AMs were isolated by BAL, seeded in RPMI complete medium in non-tissue culture treated 12-well plates and incubated at 37°C to allow the AMs to adhere. After 4h the medium was exchanged to remove dead cells, debris and cells that were not adherent. AMs were then cultured in RPMI complete medium supplemented with 20ng/ml CSF2 (Stemcell Technologies) for 7 days to expand the cells. Every 2 to 3 days the medium was replaced. At day 7 cells were treated with 1000units/ml of mouse IFNα (Miltenyi) to induce Cre-mediated recombination. IFNα treatment was repeated twice in two-day intervals. At day 14 all cells were harvested for analysis.
treatment