SAMN28232292
External Id
Biomedicine, University of Turku / Medicity
INSDC center name
2022-09-16T00:00:00Z
INSDC first public
2022-09-16T02:10:19.317Z
INSDC last update
SRS12984568
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS12984568
SRA accession
CD11c +/Cre Cebpb flox/flox
genotype
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
donor bone marrow-derived alveolar macrophages adult
source_name
Cebpbtm1.1Elgaz/J crossed with Tg(Itgax-cre,-EGFP)4097Ach/J
strain
C57BL/6
strain background
whole lung tissue
tissue
chimera_5w alveolar macrophages CD45.2-CD11cCre Cebpb KO rep3 [lung_2.2_5w_3]
title
For the generation of BM chimeras, 8-12 weeks old recipient mice (CD45.1/2) were lethally irradiated. On the following day, the recipients were reconstituted with 5x10^6 BM cells, which were a mixture of 33% WT (CD45.1/1) and 66% CD11c-Cre Cebpbfl/fl (CD45.2/2; CD11cKO) BM cells, by tail vein injection. The animals received an antibiotic treatment with Enrofloxacin in their drinking water for 10 days after irradiation. BM chimeras were analyzed 5 and 10 weeks after transfer.
treatment