MIGS/MIMS/MIMARKS.water
BioSampleModel
MIGS.eu
BioSampleModel
2022-09-27
ENA-FIRST-PUBLIC
2022-09-27
ENA-LAST-UPDATE
SAMN31023933
External Id
pda|bruce_cahoon
INSDC center alias
pda|bruce_cahoon
INSDC center name
2022-09-27T01:49:37Z
INSDC first public
2022-09-27T01:49:37Z
INSDC last update
public
INSDC status
SRS15242758
SRA accession
Cryptomonas pyrenoidifera 2021 isolate
Submitter Id
779m
altitude
NCBI
broker name
2021-04
collection_date
Surface
depth
Temperate Mid-Southern Appalachian Mountains
env_broad_scale
Shallow ephemeral pool surrounded by vegetation and located on reclaimed mine land
env_local_scale
stagnant shallow fresh water
env_medium
USA: Wise County, Virginia
geo_loc_name
Water was collected from the pool and used to make Pond Agar medium (unfiltered pond water, 1.5% bacto-agar, pH 7.4, buffered with 0.02M Tris) to culture micro-algae. Microeukaryotes were collected by centrifuging two liters of the collected water at 3000xg for 15 minutes, pellets were resuspended in a small amount of sterile distilled water, spread onto Pond Agar, and incubated at room temperature with natural light (windowsill). Plates were screened every few days using a dissecting microscope to identify cells that appeared to be multiplying under these conditions. These cells were transferred to new Pond Agar plates using a flame sterilized inoculation loop and/or a lab spatula. The isolate was re-screened and transferred over the course of several weeks to produce a monoculture.
isol_growth_condt
01
isolate
36.9781 N 82.5558 W
lat_lon
Cryptomonas pyrenoidifera
http://purl.obolibrary.org/obo/NCBITaxon_233184
organism
Crypt_01_2021
strain
Cryptomonas pyrenoidifera
title