SAMN21239331
External Id
London Institute of Medical Sciences, UKRI
INSDC center name
2022-10-01T00:00:00Z
INSDC first public
2022-10-01T01:48:27.070Z
INSDC last update
SRS10025546
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS10025546
SRA accession
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Cells arrested in Cdc20 metaphase and Mcd1 cleaved with TEV protease replicate 2
source_name
Cells arrested in Cdc20 metaphase and Mcd1 cleaved with TEV protease replicate 2
title
To arrest cells in metaphase (Cdc20), cells carrying the CDC20 gene under methionine repressible promoter MET3 (MET3-CDC20) were grown overnight in minimal media lacking methionine and raffinose 2%. The day after, cells were arrested by washing the cells and resuspending them in rich media containing 5mM methionine for 3 hours at 23⁰C. Galactose 2% was added to the media and samples for HiC were collected after 90 minutes
treatment