SAMN21239336
External Id
London Institute of Medical Sciences, UKRI
INSDC center name
2022-10-01T00:00:00Z
INSDC first public
2022-10-01T01:48:27.593Z
INSDC last update
SRS10025544
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS10025544
SRA accession
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Cells arrested in Cdc20 metaphase
source_name
Cells arrested in Cdc20 metaphase
title
To arrest cells in metaphase (Cdc20), cells carrying the CDC20 gene under methionine repressible promoter MET3 (MET3-CDC20) were grown overnight in minimal media lacking methionine. The day after cells were arrested by washing the cells and resuspending them in rich media containing 5mM methionine for 3 hours at 23⁰C
treatment