SAMN21924230
External Id
Manchester Metropolitan University
INSDC center name
2022-10-01T00:51:50.467Z
INSDC first public
2022-10-01T00:51:50.467Z
INSDC last update
SRS10396392
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS10396392
SRA accession
2014/2015
collection_date
40g honey sampled from managed Apis mellifera hive.
description
United Kingdom
geo_loc_name
Apis mellifera
host
Honey sampled from managed Apis mellifera hives in Greater Manchester
isolation_source
53.686795 N 2.250133 W
lat_lon
honey metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1561046
organism
Traditional honey collection method. Honey collected from multiple frames in the hive.
samp_collect_device
4x(10g honey diluted in 25ml H2O). Samples centrifuged at 16,000xg for 30 minutes. Collected pollen disrupted using laboratory tissue homogeniser and proteinase K. Disrupted pollen from each replicate pooled and DNA extracted using Qiagen DNeasy Plant Mini. Amplified using ITS2 PCR primers and sequenced using Illumina HiSeq.
samp_mat_process
40g honey
samp_size
Cowpe_ITS2
title