SAMD00029366
External Id
Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences , University of Tokyo
INSDC center name
2021-08-08T00:00:00Z
INSDC first public
2022-10-09T03:24:20.370Z
INSDC last update
DRS200439
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS200439
SRA accession
BHY173(pACYC184deltatet::ecoP15Imod).Stationary phase. Strand-specific mRNA-seq. This strain was grown in Lennox LB to mid-exponential phase (O.D.600 =0.5) and stationary phase (O.D.600 =OD 1.1) in three different batches. Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit or Gram-negative bacteria(Epicentre). cDNA library was constructed using Sure Select Strand Specific RNA library kit (Agilent technology). The indexed cDNA libraries were pooled and sequenced in HiSeq 2500 platform (Illumina).
description
Escherichia coli str. K-12 substr. MG1655
http://purl.obolibrary.org/obo/NCBITaxon_511145
organism
BHY193_stat_SS
sample name
BHY173
http://www.ebi.ac.uk/efo/EFO_0006539
strain
MG1655
http://www.ebi.ac.uk/efo/EFO_0002234
substrain
BHY193_stat_SS
title