2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
SAMN26263586
External Id
GSM5918128
External Id
2022-07-26T01:35:22Z
INSDC first public
2022-07-26T01:35:22Z
INSDC last update
public
INSDC status
SRS12109117
SRA accession
GSM5918128
Submitter Id
6102021
batch_date
NCBI
broker name
Neonatal_foreskin_fibroblast_2
cell_line
human
common name
AG21859
coriell_id
ambient
culture_oxygen
6
days_in_culture
0 years
donor_age
male
donor_sex
Fibroblasts were maintained in Ham's F12/DMEM 1:1 media supplemented with 10% v/v fetal bovine serum. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
12.63
population_doublings
Foreskin
source_name
Baseline profiling
subexperiment
NFF2_1
title