2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
SAMN26263600
External Id
GSM5918114
External Id
2022-07-26T01:35:22Z
INSDC first public
2022-07-26T01:35:22Z
INSDC last update
public
INSDC status
SRS12109103
SRA accession
GSM5918114
Submitter Id
10212021
batch_date
NCBI
broker name
Adult_skin_fibroblast_1
cell_line
human
common name
AG16146
coriell_id
ambient
culture_oxygen
43
days_in_culture
31 years
donor_age
male
donor_sex
Fibroblasts were maintained in Eagle's MEM with Earle's salts with 10% v/v fetal bovine serum. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
18.51
population_doublings
Skin, arm
source_name
b
subculture
Baseline profiling
subexperiment
ASF1_3
title