2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
GSM5918162
External Id
SAMN26263552
External Id
2022-07-26T01:35:22Z
INSDC first public
2022-07-26T01:35:22Z
INSDC last update
public
INSDC status
SRS12109151
SRA accession
GSM5918162
Submitter Id
10212021
batch_date
NCBI
broker name
Fetal_skin_fibroblast_1
cell_line
human
common name
AG06561
coriell_id
ambient
culture_oxygen
221
days_in_culture
16 fetal weeks
donor_age
female
donor_sex
TERT
expression_vector
Fibroblasts were maintained in Eagle's MEM with Earle's salts and non-essential amino acids with 15% v/v fetal bovine serum. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
115.81
population_doublings
Skin, sacrum
source_name
2a
subculture
TERT immortalization
subexperiment
FSF1_TERT_11
title
Low-PD primary fibroblasts were transduced with purified lentiviral particles containing expression vectors encoding human Telomerase Reverse Transcriptase (TERT) and hygromycin resistance marker. Following selection with 250ug/ml hygromycin B, cells were serially cultured in perpetuity, in triplicate.
treatment protocol