2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
SAMN26263608
External Id
GSM5918106
External Id
2022-07-26T01:35:21Z
INSDC first public
2022-07-26T01:35:21Z
INSDC last update
public
INSDC status
SRS12109095
SRA accession
GSM5918106
Submitter Id
10212021
batch_date
NCBI
broker name
Adult_vascular_smooth_muscle_cell_1
cell_line
human
common name
AG11546
coriell_id
ambient
culture_oxygen
26
days_in_culture
19 years
donor_age
male
donor_sex
Vascular smooth muscle cells were maintained in Medium 199 with 1X GlutaMAX, 0.02mg/ml endothelial cell growth supplement, 0.05 mg/ml sodium heparin, and 10% v/v fetal bovine serum on plates pre-coated with gelatin. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
35.83
population_doublings
Iliac vein
source_name
b
subculture
Baseline profiling
subexperiment
AVSMC1_3
title