2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
GSM5918089
External Id
SAMN26263625
External Id
2022-07-26T01:35:21Z
INSDC first public
2022-07-26T01:35:21Z
INSDC last update
public
INSDC status
SRS12109078
SRA accession
GSM5918089
Submitter Id
10212021
batch_date
NCBI
broker name
Fetal_skin_fibroblast_1
cell_line
human
common name
AG06561
coriell_id
ambient
culture_oxygen
21
days_in_culture
16 fetal weeks
donor_age
female
donor_sex
Fibroblasts were maintained in Eagle's MEM with Earle's salts and non-essential amino acids with 15% v/v fetal bovine serum. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
23.93
population_doublings
Skin, sacrum
source_name
b
subculture
Baseline profiling
subexperiment
FSF1_2
title