2022-07-26
ENA-FIRST-PUBLIC
2022-07-26
ENA-LAST-UPDATE
SAMN26263622
External Id
GSM5918092
External Id
2022-07-26T01:35:21Z
INSDC first public
2022-07-26T01:35:21Z
INSDC last update
public
INSDC status
SRS12109081
SRA accession
GSM5918092
Submitter Id
6102021
batch_date
NCBI
broker name
Fetal_skin_fibroblast_1
cell_line
human
common name
AG06561
coriell_id
ambient
culture_oxygen
65
days_in_culture
16 fetal weeks
donor_age
female
donor_sex
Fibroblasts were maintained in Eagle's MEM with Earle's salts and non-essential amino acids with 15% v/v fetal bovine serum. Triplicate cultures derived from the same parent plate or vial obtained from Coriell were maintained in parallel through replicative senescence, which was defined in this study as drastically slowed growth (inability to reach near-confluence at 14 days after previous passage) or viable fraction of cells falling below 60%. Passaging occurred as cells became approximately 90% confluent.
growth protocol
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
50.66
population_doublings
Skin, sacrum
source_name
b
subculture
Baseline profiling
subexperiment
FSF1_5
title