Beta_lactam
Abx_class
1785.11
Avg_insert_size (bp)
SAMN31371967
External Id
P01
FMG_library_id
Washington University School of Medicine
INSDC center name
2022-10-24T00:50:12.660Z
INSDC first public
2022-10-24T00:50:12.660Z
INSDC last update
SRS15478592
INSDC secondary accession
live
INSDC status
2.63-2.91
Library_size (GB)
8
MIC (ug/ml)
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS15478592
SRA accession
P01_ATM
Selection_id
P01SR2_ATM
SeqRunID
Aztreonam
antibiotic_name
2012-08/2015-12
collection_date
50
colony_count
Peru: Cusco
geo_loc_name
Homo sapiens
host
stool
isolation_source
13.518840 S 71.975040 W
lat_lon
human gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_408170
organism
Metagenomic DNA from 10 random samples were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli indicator hosts. These E. coli transformant libraries were then screened against an antibiotic at a selective concentration. Resistant clones were collected from solid media and were sequenced using Nextera protocol on Illumina NextSeq500.
samp_mat_process
Metagenome or environmental sample from human gut metagenome
title