MIGS/MIMS/MIMARKS.water
BioSampleModel
MIGS.eu
BioSampleModel
2022-10-28
ENA-FIRST-PUBLIC
2022-10-28
ENA-LAST-UPDATE
SAMN31462905
External Id
pda|bruce_cahoon
INSDC center alias
pda|bruce_cahoon
INSDC center name
2022-10-28T01:43:00Z
INSDC first public
2022-10-28T01:43:00Z
INSDC last update
public
INSDC status
SRS15540319
SRA accession
Gp_VA_01
Submitter Id
779m
altitude
NCBI
broker name
2021-05
collection_date
surface
depth
Shallow (<=500cm) stagnant ephemeral pool approximately 200 m2 in size.
env_broad_scale
It is located on a site that had been surface mined for coal in the 1980s and 1990s, reclaimed following the guidelines at that time, and is now part of a preserve managed by the University of Virginias College at Wise. This pool spontaneously formed in a low-lying area and was not a purposefully constructed retention pond. It is bordered along one side by the shrub Eleagnus umbellate, an introduced/invasive species previously used for surface mine reclamation, which provides partial shade. This body of water supports local fauna and amphibian egg clutches are often found there in the early spring.
env_local_scale
water
env_medium
USA: Wise, Virginia
geo_loc_name
Water collected from the pool was used to produce Pond Agar medium to culture micro-algae, (0% bacto-agar, pH 7.4, buffered with 00M Tris). Microeukaryotes were collected by centrifuging two liters of the collected water at 3000xg for 15 minutes, pellets were resuspended in a small amount of sterile distilled water, spread onto Pond Agar, and incubated at room temperature with natural light (windowsill). Plates were screened every few days using a dissecting microscope to identify small isolated colonies. Colonies were transferred to new Pond Agar plates using a flame sterilized inoculation loop and/or a lab spatula. Isolates were re-screened and transferred over the course of several weeks to produce culture plates with a single alga.
isol_growth_condt
36.58 N 82.33 W
lat_lon
Gomphonema parvulum
http://purl.obolibrary.org/obo/NCBITaxon_97227
organism
VA_01
strain
Gomphonema parvulum
title