SAMD00189819
External Id
Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
INSDC center name
2022-10-31T00:00:00Z
INSDC first public
2022-10-31T09:03:25Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
Synchronized L1 larvae of KH1668: smg-2 (yb979) I were fed with 10%-diluted supernatant of BugBuster Protein Extraction Reagent (Novagen) lysate of E. coli strain DGF-298W delta 100::rev delta 234::SC (NBRP-E.coli at NIG) in S-complete medium supplemented with 4 mM 4-thiouracil (4TU) (Tokyo Chemical Industry Co., Ltd.) and 20 mM choline chloride (nacalai tesque) for 60 min at 20 degree Celsius. The worms were harvested and washed with M9 and total RNAs were extracted from the whole bodies by using RNeasy Plus Mini (QIAGEN). 4TU-labeled RNAs were biotinylated with MTSEA biotin-XX (Biotium) and purified by using Streptavidin Mag Sepharose (GE-healthcare). Ribosomal RNAs were then removed with Ribo-Zero Gold rRNA Removal Kit (Epidemiology) (illumina) and the remaining RNAs were subjected to RNA-seq library preparation with SMARTer Stranded RNA-Seq Kit (Clontech).
description
L1
dev stage
smg-2 (yb979) I
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
Nonsense-mediated mRNA decay (NMD)-deficient
phenotype
w24808
sample name
KH1668
strain
whole
tissue
w24808_KH1668_L1_KHK36 BugBuster sup_Rep2_Index6
title