SAMN31286417
External Id
USDA ARS
INSDC center name
2022-10-28T00:00:00Z
INSDC first public
2022-10-28T15:43:50.520Z
INSDC last update
SRS15570246
INSDC secondary accession
live
INSDC status
Pathogen.env
NCBI submission model
Pathogen.env.1.0
NCBI submission package
SRS15570246
SRA accession
USDA ARS AFRS
collected_by
2021-12
collection_date
Fusarium avenaceum has a large host range and has been reported to cause disease on over 80 genera of plants. A F. avenaceum isolate was obtained in December 2021 from a decaying European pear (Pyrus communis L.) Dawn with brown, circular, watery, and sunken lesions that was in cold storage at the USDA Appalachian Fruit Research Station located in Kearneysville, West Virginia. Koch's postulates were conducted using six Bartlett pear fruits that were wound-inoculated with mycelial plugs of the fungus. Brown, circular, watery, and sunken lesions developed within 48 hours after inoculation and incubation at 25 C, while PDA plug inoculated fruit were symptomless. The fungus was reisolated from infected pears and was morphologically identical to the original isolate. Identity of the isolate was confirmed through DNA extraction followed by amplification and sequencing of the ITS and TEF1 gene region. The ITS amplicon (OP007197) matched multiple Fusarium spp. with 100% identity and 100% query coverage including F. avanaceum (KY910897 and KU936793). The TEF1 amplicon (OP007198) showed 98-99% identity and 98-100% query coverage with F. avenaceum isolates KM189442 and MK512754. F. avenaceum can produce mycotoxins, which is a concern for the fruit processing industry. This is the first report of F. avenaceum causing postharvest decay of European pear in the Mid-Atlantic region of the United States.
description
USA:Kearneysville, West Virginia
geo_loc_name
European pear (Pyrus communis L.) Dawn with brown, circular, watery, and sunken lesions was observed in cold storage
isolation_source
39.3552 N 77.8766 W
lat_lon
Fusarium avenaceum
http://purl.obolibrary.org/obo/NCBITaxon_40199
organism
WV21P1A
strain
Fusarium avenaceum isolated from a post-harvest rotted pear
title