SAMN28871182
External Id
Pai lab, RNA Therapeutics Institute, UMASS Medical School
INSDC center name
2022-10-29T00:00:00Z
INSDC first public
2022-10-29T00:37:32.764Z
INSDC last update
SRS13297855
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS13297855
SRA accession
GAA repeat (330,380) on intron 1 of FXN
mutations
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
GM03816 cells
source_name
Fibroblast
tissue
ASO-NTC_rep1
title
3000 cells were seeded and cultured at 37C with 5% CO2. After 6-9hs, lipofectamine RNAiMAX (Invitrogen) was used to transfect a non-targeting ASO with a 1/4 of lipofectamine:oligonucleotide ratio of the manufacturer'srecommended protocol. The cells were incubated with full media and transfection reagents for 72h. Three independent replicates were done for this treatment.
treatment