SAMD00089453
External Id
Nihon BioData Corporation
INSDC center name
2019-02-06T00:00:00Z
INSDC first public
2022-10-30T13:44:34.363Z
INSDC last update
DRS087823
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS087823
SRA accession
Gibco (Thermo Fisher Scientific)
biomaterial provider
CHO-S
cell line
Suspension cells
http://purl.obolibrary.org/obo/BTO_0000221
cell type
2017-05-01
collection date
CHO-S Cells (cGMP banked) and Media Kit (Gibco, Thermo Fisher Scientific), Lot: 556395-WCB1
description
Viable cell density: 3.94 x 10^6 cells/mL, Viability: 80.4%
growth condition
CHO-S cells were grown in 20 ml of Gibco CD-CHO medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 400 microliter of 200 mM Ala-Gln (final concentration, 4 mM; Sigma-Aldrich, St. Louis, MI, USA) and 40 microliter of Gibco Anti-Clumping Agent (final concentration, 0.2% (v/v); Thermo Fisher Scientific) in a 125 mL flask. The flask was shaken (120 rpm) at 37 degree Celsius in a humidified 5% CO2 atmosphere.
growth_protocol
Cricetulus griseus
http://purl.obolibrary.org/obo/NCBITaxon_10029
organism
Passage 17, 7 days in culture
passage_history
CHO-S_F8F
sample name
Rep 3
technical replicate
Chinese Hamster Ovary (CHO-S) cells, 7 days in culture, time lag from harvesting cells to protecting RNA: 60 min, technical replicate: 3
title