SAMN20157339
External Id
INRAE
INSDC center name
2022-11-03T00:50:13Z
INSDC first public
2022-11-03T00:50:13Z
INSDC last update
SRS9446055
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS9446055
SRA accession
IGEPP INRAE
biomaterial_provider
DNA extraction was performed from parthenogenetic embryos dissected from 20 female adults of this X6-2 OP lineage. Embryos were grounded in lysis buffer using a potter. The lysis solution was then used in a Phenol protocol but using high vacuum grease silicone to separate aqueous and organic phases without pipetting. We also reduced the centrifuge speed to preserve DNA. The DNA was directly precipitated with isopropanol and then resuspended in ultra-pure sterilized water. DNA concentration was measured with Qubit fluorometer and DNA length (range 17kb-100kb) was assessed with both a Pulse Field Gel Electrophoresis and a Femto Pulse Run.
description
parthenogenetic embryo
dev_stage
X6-2 OP
isolate
Acyrthosiphon pisum
http://purl.obolibrary.org/obo/NCBITaxon_7029
organism
female
sex
LSR1
strain
whole body
tissue
Acyrthosiphon pisum LSR1 from obligate parthenogenic lineages OP
title