SAMN31621269
External Id
Jiangsu University
INSDC center name
2022-11-07T00:00:00Z
INSDC first public
2022-11-08T02:27:17.427Z
INSDC last update
SRS15700140
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS15700140
SRA accession
2021
collection_date
For viral metagenomic analysis, 192 samples from 36 death ducks were randomly collected from the same farm in Shandong Province, China, 2021. The 192 samples were randomly pooled into eleven supernatant pools, each including 16-18 samples. Each supernatant pool was filtered with a 0.45um filter to remove particles the size of bacterial and eukaryotic cells and the filtrates enriched in viral particles were treated with DNase and RNase to digest unprotected nucleic acid. The remaining total viral RNA and DNA were extracted by using the QIAamp viral RNA Minikit according to the protocol. Eleven libraries were then constructed using Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced using the MiSeq Illumina platform with 250 bases paired ends with dual barcoding for each pool.
description
China: Shandong province
geo_loc_name
Duck
host
heart/liver/spleen/lung/kidney/gut
isolation_source
bird metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1833763
organism
Metagenome or environmental sample from Duck metagenome
title