SAMN31881048
External Id
University of Maryland School of Medicine
INSDC center name
2022-11-26T00:00:00Z
INSDC first public
2022-11-26T23:57:18.973Z
INSDC last update
SRS15874158
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS15874158
SRA accession
7 weeks
age
16-Jul-2021
birth_date
Jax
breed
2021-09-07
collection_date
Jax_sc
condition
07-Sep-2021
death_date
skin biopsies were incubted in dispase solution for 2.5 hours at 37°C. Epidermal sheets were separated from dermal layer using forceps. Epidermal sheets were then incubated in a collagenase digestion solution for 1 hour at 37°C. Cells were resuspended using a wide-bore pipette tip, passed through a 40uM cell strainer, and washed with RPMI+10% FBS. Cells were counted, stained with 5 µl of 7-AAD per million cells counted, and FACS sorted.
description
mature stage
dev_stage
dorsal neck
isolation_source
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
skin
sample_type
male
sex
FVB/NJ
strain
skin
tissue
Jax_S3
title