SAMN23480575
External Id
Norwegian University of Life Sciences (NMBU)
INSDC center name
2022-12-01T00:51:59.157Z
INSDC first public
2022-12-01T00:51:59.157Z
INSDC last update
SRS11167667
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS11167667
SRA accession
O.W. Milvang, NMBU
collected_by
2015-12
collection_date
Lea
cultivar
first pool containing 17 late elongating plants
description
3-months old plants
dev_stage
Seeds of red clover (Trifolium pratense L., cultivar ‘Lea’ (Graminor Ltd.)) were sown in a greenhouse in September 2015 and grown at approximately 16 °C with a 20 h photoperiod (natural light supplied with 90 µmol m-2 s-1 PAR (HPQ/HTI-P lamps)). The number of days from sowing until the first elongating internode was 2 cm long (days to elongation, DTE) was recorded for each plant.
growth_protocol
Trifolium pratense
http://purl.obolibrary.org/obo/NCBITaxon_57577
organism
late elongating
phenotype
subpopulation of cultivar Lea; first pool containing 17 late elongating plants
population
The 52 earliest and 52 latest individuals (excluding non-elongating individuals) were selected for genotyping by pool-GBS. Pools were created as follows: DNA was extracted from leaf tissue of each individual with the DNeasy 96 Plant kit (Qiagen). The 52 individuals in each of the two phenotypic groups (early, late) were randomly divided into three subgroups of 17-18 individuals and equal amounts of DNA from each individual in each subgroup was combined in a pool, creating a total of six DNA pools. This pool contains the first subgroup (replicate 1) of 17 late elongating individuals.
sample_type
young leaves
tissue
late_elongating_pool_rep1
title