Pathogen.cl
BioSampleModel
2022-12-06
ENA-FIRST-PUBLIC
2022-12-06
ENA-LAST-UPDATE
SAMN31996488
External Id
pda|valenciatibb@wisc.edu
INSDC center alias
pda|valenciatibb@wisc.edu
INSDC center name
2022-12-06T01:17:13Z
INSDC first public
2022-12-06T01:17:13Z
INSDC last update
public
INSDC status
Biological replicate 1
Replicate
SRS15967937
SRA accession
sars_cov_2_omicron_BA5_stock_lot01152022_0600_rep01
Submitter Id
NCBI
broker name
BIOQUAL
collected_by
2021-11
collection_date
not provided
culture_collection
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/MD-HP20874/2021 was isolated from a nasal swab in Maryland, USA, on November 27, 2021. NR-56462 lot 70049427 was produced by infecting Homo sapiens lung adenocarcinoma epithelial cells (Calu-3; ATCC® HTB-55TM) with the deposited material and incubating in Eagle's Minimum Essential Medium (ATCC® 30-2003TM) supplemented with 2% fetal bovine serum (ATCC® 30-2020TM) for 7 days at 37°C with 5% CO2. The cells and supernatant were spin-clarified at 1500 × g for 10 minutes at 4°C.
description
USA: Maryland
geo_loc_name
Homo sapiens
host
COVID-19
host_disease
hCoV-19/USA/MD-HP20874/2021
isolate
Severe acute respiratory syndrome coronavirus 2
http://purl.obolibrary.org/obo/NCBITaxon_2697049
organism
Omicron Lineage BA.5
strain
SARS-CoV-2 Omicron BA.5 Stock Lot# 01152022-0600 Rep01
title