SAMN25282229
External Id
Universitat de Barcelona
INSDC center name
2022-12-18T00:00:00Z
INSDC first public
2022-12-18T16:12:02.339Z
INSDC last update
SRS11775763
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS11775763
SRA accession
2017
collection_date
Schmidtea mediterranea clonal strain BCN-10 animals were starved for at least 7 days prior to any conducted experiment. Asexual animals were cultured in glass containers and Petri dishes for experiments in planarian artificial medium (PAM) water at 20C in the dark. Animals were regularly fed twice per week with organic cow liver. Wild type animals were amputated posterior to the mouth; collection of wound samples (blastema and post-blastema region) anterior to tail fragments was performed at 12h of regeneration after amputation. A total of 2000 anterior and posterior blastemas were used. Groups of 100 blastemas were done at one time.
description
Asexual adult worms
dev_stage
Spain: Barcelona
geo_loc_name
asexual clonal strain BCN-10
isolate
Animals cultured in glass containers and Petri dishes in planarian artifitial medium (PAM) at 20C in the dark, fed twice per week with organic cow liver.
isolation_source
Schmidtea mediterranea
http://purl.obolibrary.org/obo/NCBITaxon_79327
organism
Wound region (blastema and post-blastema region anterior to tail fragments, 12h regeneration, cut posterior to the mouth, wild type animals).
tissue
Wnt/Bcat signalling chromatin remodeling planarian regeneration: H3K27ac ChIP-seq wt 12h anterior blastemas
title