SAMN25282226
External Id
Universitat de Barcelona
INSDC center name
2022-12-18T00:00:00Z
INSDC first public
2022-12-18T16:12:02.356Z
INSDC last update
SRS11775776
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS11775776
SRA accession
2017
collection_date
Schmidtea mediterranea clonal strain BCN-10 animals were starved for at least 7 days prior to any conducted experiment. Asexual animals were cultured in glass containers and Petri dishes for experiments in planarian artificial medium (PAM) water at 20C in the dark. Animals were regularly fed twice per week with organic cow liver. gfp RNAi control animals were amputated posterior to the mouth; collection of wound samples (blastema and post-blastema region) posterior to trunk fragments was performed at 12h of regeneration after amputation. 20 animals were used per biological replicate.
description
Asexual adult worms
dev_stage
Spain: Barcelona
geo_loc_name
asexual clonal strain BCN-10
isolate
Animals cultured in glass containers and Petri dishes in planarian artifitial medium (PAM) at 20C in the dark, fed twice per week with organic cow liver.
isolation_source
Schmidtea mediterranea
http://purl.obolibrary.org/obo/NCBITaxon_79327
organism
Wound region (blastema and post-blastema region posterior to trunk fragments, 12h regeneration, cut posterior to the mouth, gfp RNAi control animals).
tissue
Wnt/Bcat signalling chromatin remodeling planarian regeneration: ATACseq control 12h posterior blastemas
title