SAMN25282210
External Id
Universitat de Barcelona
INSDC center name
2022-12-18T00:00:00Z
INSDC first public
2022-12-18T16:12:02.418Z
INSDC last update
SRS11775769
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS11775769
SRA accession
2017
collection_date
Schmidtea mediterranea clonal strain BCN-10 animals were starved for at least 7 days prior to any conducted experiment. Asexual animals were cultured in glass containers and Petri dishes for experiments in planarian artificial medium (PAM) water at 20C in the dark. Animals were regularly fed twice per week with organic cow liver. Control animals were injected for one round at 1500ng/ul with gfp dsRNA and amputated at the post-pharyngeal level. Wound samples (blastema and post-blastema) were obtained at 0h of regeneration after amputation. Three biological replicates were used per time point; each biological replicate was composed of eight animal fragments.
description
Asexual adult worms
dev_stage
Spain: Barcelona
geo_loc_name
asexual clonal strain BCN-10
isolate
Animals cultured in glass containers and Petri dishes in planarian artifitial medium (PAM) at 20C in the dark, fed twice per week with organic cow liver.
isolation_source
Schmidtea mediterranea
http://purl.obolibrary.org/obo/NCBITaxon_79327
organism
Wound region (blastema and post-blastema region posterior to the trunk, 0h regeneration, cut posterior to the mouth, gfp RNAi control animals).
tissue
Wnt/Bcat signalling chromatin remodeling planarian regeneration: RNAseq control 0h
title