SAMN32323994
External Id
Khavari Lab, Stanford University
INSDC center name
2022-12-23T00:50:11.117Z
INSDC first public
2022-12-23T00:50:11.117Z
INSDC last update
SRS16219704
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS16219704
SRA accession
day 10
age
H9
cell line
neuron
cell type
Stem cells were plated in clumps in the presence of Y27632 (10mg/ml, 1000x, Axon MedChem). The hES colonies were allowed to grow in mTESR for another day before the media was switch to differentiation media (1X N2, 1X B27, DMEM/F12: Neurobasal=1:1 (Invitrogen), 0.1mM Ascorbic acid (Sigma)) with small molecules [SB431542, LDN193189 and CHIR99021 (Final concentration: 10µM, 100nM and 3µM from Stemgent and Tocris)]. To obtain anterior or posterior neural stem cells, SB431542/LDN194189 and SB431542/LDN193189/CHIR99021 were added respectively. The cells were allowed to differentiate in the media for 6 days before they were dissociated with Accutase and plated at the density of 1 million cells per well of 6 wells for infection. For Ngn2 day 2 post-dox induction cells, TetO-Ngn2-t2a-puro and FUW-rtTA were infected at day 0 in differentiation media followed by adding doxycycline and puromycin the next day. The cells were selected for two days in puromycin before collection for downstream HiChIP experiment.
growth
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
H9
source_name
mpra_N-D10_3
title