SAMN32323999
External Id
Khavari Lab, Stanford University
INSDC center name
2022-12-23T00:50:12.810Z
INSDC first public
2022-12-23T00:50:12.810Z
INSDC last update
SRS16219699
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS16219699
SRA accession
day 4
age
H9
cell line
neuron
cell type
Stem cells were plated in clumps in the presence of Y27632 (10mg/ml, 1000x, Axon MedChem). The hES colonies were allowed to grow in mTESR for another day before the media was switch to differentiation media (1X N2, 1X B27, DMEM/F12: Neurobasal=1:1 (Invitrogen), 0.1mM Ascorbic acid (Sigma)) with small molecules [SB431542, LDN193189 and CHIR99021 (Final concentration: 10µM, 100nM and 3µM from Stemgent and Tocris)]. To obtain anterior or posterior neural stem cells, SB431542/LDN194189 and SB431542/LDN193189/CHIR99021 were added respectively. The cells were allowed to differentiate in the media for 6 days before they were dissociated with Accutase and plated at the density of 1 million cells per well of 6 wells for infection. For Ngn2 day 2 post-dox induction cells, TetO-Ngn2-t2a-puro and FUW-rtTA were infected at day 0 in differentiation media followed by adding doxycycline and puromycin the next day. The cells were selected for two days in puromycin before collection for downstream HiChIP experiment.
growth
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
H9
source_name
mpra_N-D4_1
title