SAMN22788967
External Id
ICAR-Indian Institute of Rice Research
INSDC center name
2022-12-25T00:50:16.040Z
INSDC first public
2022-12-25T00:50:16.040Z
INSDC last update
SRS11018773
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS11018773
SRA accession
20 Day Old Seedlings
age
RNA-seq of Rice (cv. BPT 5204) Inoculated With Bradyrhizobium japonicum at 240 HRS Post Inoculation (R2 - Replication 2)
aliquot
ICAR-Indian Institute of Rice Research
biomaterial_provider
Dr. P. C. Latha
collected_by
2020-02
collection_date
BPT 5204
cultivar
RNA-seq Based Molecular Response of Rice (cv. BPT 5204) to inoculation with Bradyrhizobium japonicum at 240 HRS Post Inoculation (R2 - Replication 2)
description
Seedling stage
dev_stage
India: Hyderabad
geo_loc_name
Germinated rice seedlings (cv. BPT 5204) were aseptically grown (5 days) in a hydroponics assembly with Hoagland’s (N+) medium at a temperature of 28 °C and a photoperiod of 16 h. The growth medium was replaced with nitrogen-free Hoagland’s medium and after acclimatization of the seedlings for 5 days, Bradyrhizobium japonicum bacterial culture was inoculated (1 x 106 CFU/ml). Following co-cultivation of rice seedlings and bacteria for 10 days (240 hours post-inoculation), rice root tissue samples were harvested for RNA extraction and subsequent dual transcriptome analysis.
growth_protocol
Oryza sativa
http://purl.obolibrary.org/obo/NCBITaxon_4530
organism
Plant sample
sample_type
28 °C
temp
Root
tissue
RNA-seq of Rice (cv. BPT 5204) Inoculated With Bradyrhizobium japonicum at 240 HRS Post Inoculation (R2 - Replication 2)
title
Rice (cv. BPT 5204) inoculated with Bradyrhizobium japonicum (RB-R2) at 240 HRS Post Inoculation
treatment