SAMN32519257
External Id
University of California, San Francisco
INSDC center name
2022-12-31T00:00:00Z
INSDC first public
2022-12-31T12:14:27.943Z
INSDC last update
SRS16328704
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS16328704
SRA accession
54.84
age
GRCh38
assembly
Raleigh Lab UCSF Helen Diller Family Comprehensive Cancer Center 1450 3rd Street, San Francisco 94174
biomaterial_provider
Flash frozen archived schwannoma specimens were minced with sterile Bard-Parker #10 surgical scalpels (Aspen Surgical) and mechanically dissociated with a Pestle Tissue Grinder (size A, Thomas Scientific) in ice cold lysis buffer consisting of 0.32 M sucrose, 5 mM CaCl2, 3 mM MgAc2, 0.1 mM EDTA, 10 mM Tris-HCl, 1 mM DTT and 0.1% Triton X-100 in DEPC-treated water. Sucrose solution consisting of 1.8 M sucrose, 3 mM MgAc2, 1 mM DTT and 10 mM Tris-HCl in DEPC-treated water was added to the bottom of the lysis solution in ultracentrifuge tubes (Beckman Coulter) to form a gradient, which was ultracentrifuged at 107,000g for 2.5 hours at 4°C. Nuclei pellets were resuspended in phosphate buffered saline and sequentially filtered twice in 30 μm strainers (Miltenyi Biotec). Isolated nuclei were assessed with DAPI staining and loaded onto a 10x Chromium controller using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10x Genomics).
design_description
sn_VS_S14_S10_L001_R1_001.fastq.gz
filename
sn_VS_S14_S10_L002_R1_001.fastq.gz
filename2
sn_VS_S14_S10_L001_R2_001.fastq.gz
filename3
sn_VS_S14_S10_L002_R2_001.fastq.gz
filename4
sn_VS_S14_S10_L001_I1_001.fastq.gz
filename5
sn_VS_S14_S10_L002_I1_001.fastq.gz
filename6
fastq
filetype
Illumina NovaSeq 6000
instrument_model
schwannoma
isolate
sn_VS_S14_S10
library_ID
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
ILLUMINA
platform
male
sex
tumor
tissue
snRNA_S14
title