0.998387214
2pool_decay_model_fit_R2
NW
Aspect
cDNA
DNA_type_sequenced
7
Densiometer.open
SAMN32271544
External Id
TAGTGTAG
Fwd_Primer_Barcode
University of Wisconsin-Madison
INSDC center name
2022-12-31T00:50:55.357Z
INSDC first public
2022-12-31T00:50:55.357Z
INSDC last update
SRS16214903
INSDC secondary accession
live
INSDC status
Lib2
Library
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
19UW-WB
Project_ID
TAGCAGAC
Rev_Primer_Barcode
SRS16214903
SRA accession
19UW-WB-15-03-O-pb-cDNA
Sequencing_Sample_Name
Lower
Slope.position
no
autoclaved_after_burn_treatment
11%
avg_clay_content
70%
avg_sand_content
19%
avg_silt_content
60 kw/m^2 heat flux for 2 min after air drying soil for 1.5 months
burn_treatment
2019
collection_date
Populus_tremuloides
cur_vegetation
10cm
depth
16S sequences from burned or unburned soils
description
not collected
elev
boreal forest
env_broad_scale
boreal forest soil
env_local_scale
forest soil
env_medium
mimarks
env_package
0.097221479
fast_cycling_C_decomposition_rate
0.002155787
fractional_size_fast_cycling_C_pool
0.997057783
fractional_size_slow_cycling_C_pool
Canada: Wood Buffalo National Park
geo_loc_name
5 cm
horizon_thickness
no
inoculation_with_unburned_soil
soil
isolation_source
60.039176 N 112.879194 W
lat_lon
80.1 C
max_burn_temperature_at_9_cm_depth
449.5 C
max_burn_temperature_at_core_midpoint
Qiagen RNeasy PowerSoil Total RNA extraction kit
nucl_acid_ext
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 387
pcr_cond
6.76
ph
28.48 g
post_burn_incubation_initla_dry_soil_mass
Invitrogen SuperScript IV VILO Master Mix kit
rev_transcription
metal soil corer, 3 cm i.d., depth up to 13.5 cm
samp_collect_device
stored in aerobic conditions at room temperature
samp_mat_process
15
sampling_site_ID
24 hrs after burn treatment
sampling_timepoint
Illumina MiSeq 2x250 PE
seq_meth
0.0000228
slow_cycling_C_decomposition_rate
O
soil_horizon
16S rRNA
target_gene
V4
target_subfragment
sandy loam
texture
physical analysis hydrometer
texture_meth
19UW-WB-15-03-O-pb-cDNA
title
6.72%
tot_carbon
0.50%
tot_nitro
0.09 g/g
water_content_soil