SAMN26849603
External Id
Johns Hopkins University
INSDC center name
2022-12-31T00:58:43.590Z
INSDC first public
2022-12-31T00:58:43.590Z
INSDC last update
SRS12330673
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS12330673
SRA accession
2020-06
collection_date
A library of single-codon substitutions in CAT-I was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We diluted uninduced, exponentially-growing cultures of the libraries mixed with wildtype CAT-I into LB media containing IPTG. Cultures were allowed to grow for ~10 generations at 37°C with a 32-fold dilution after ~5 generations of growth. We plasmid prepped and determined the allele frequencies of the generation 10, replica 1 sample using deep sequencing. Data for codons 1-220 was used from these files. This was used as Replica 1 for regions 1 (codons 1-73), 2 (codons 74-146), and 3 (codons 147-220).
description
10
generation
USA: Maryland
geo_loc_name
Escherichia coli
http://purl.obolibrary.org/obo/NCBITaxon_562
organism
sequencing run 3
replicate
cell culture
sample_type
NEB 5-alpha F'Iq
strain
37°C
temp
DMS of NEB 5-alpha F'Iq: Single-codon substitutions in CAT-I, sequencing run 3, generation 10
title