SAMN26849602
External Id
Johns Hopkins University
INSDC center name
2022-12-31T00:58:43.583Z
INSDC first public
2022-12-31T00:58:43.583Z
INSDC last update
SRS12330672
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS12330672
SRA accession
2020-06
collection_date
A library of single-codon substitutions in CAT-I was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We plasmid prepped uninduced, exponentially-growing cultures of the library mixed with wildtype CAT-I and determined the allele frequencies at the start of the growth experiment as our generation 0, replica 1 sample. Data for codons 1-220 was used from these files. This was used as Replica 1 for regions 1 (codons 1-73), 2 (codons 74-146), and 3 (codons 147-220).
description
0
generation
USA: Maryland
geo_loc_name
Escherichia coli
http://purl.obolibrary.org/obo/NCBITaxon_562
organism
sequencing run 3
replicate
cell culture
sample_type
NEB 5-alpha F'Iq
strain
37°C
temp
DMS of NEB 5-alpha F'Iq: Single-codon substitutions in CAT-I, sequencing run 3, generation 0
title