0.99936761
2pool_decay_model_fit_R2
cDNA
DNA_type_sequenced
3
Densiometer.open
SAMN32271413
External Id
TAGTGTAG
Fwd_Primer_Barcode
University of Wisconsin-Madison
INSDC center name
2022-12-31T00:50:54.897Z
INSDC first public
2022-12-31T00:50:54.897Z
INSDC last update
live
INSDC status
Lib2
Library
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
19UW-WB
Project_ID
TACGAGCA
Rev_Primer_Barcode
19UW-WB-09-09-A-pb-cDNA
Sequencing_Sample_Name
Level
Slope.position
no
autoclaved_after_burn_treatment
16.50%
avg_clay_content
54%
avg_sand_content
29.50%
avg_silt_content
unburned control
burn_treatment
2019
collection_date
Pinus_banksiana
cur_vegetation
10cm
depth
16S sequences from burned or unburned soils
description
not collected
elev
boreal forest
env_broad_scale
boreal forest soil
env_local_scale
forest soil
env_medium
mimarks
env_package
0.003472302
fast_cycling_C_decomposition_rate
0.016978737
fractional_size_fast_cycling_C_pool
0.982769869
fractional_size_slow_cycling_C_pool
Canada: Wood Buffalo National Park
geo_loc_name
5 cm
horizon_thickness
no
inoculation_with_unburned_soil
soil
isolation_source
59.684454 N 112.17181 W
lat_lon
21 C
max_burn_temperature_at_9_cm_depth
21 C
max_burn_temperature_at_core_midpoint
Qiagen RNeasy PowerSoil Total RNA extraction kit
nucl_acid_ext
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 256
pcr_cond
3.88
ph
31.95 g
post_burn_incubation_initla_dry_soil_mass
Invitrogen SuperScript IV VILO Master Mix kit
rev_transcription
metal soil corer, 3 cm i.d., depth up to 13.5 cm
samp_collect_device
stored in aerobic conditions at room temperature
samp_mat_process
9
sampling_site_ID
24 hrs after burn treatment
sampling_timepoint
Illumina MiSeq 2x250 PE
seq_meth
2.45E-08
slow_cycling_C_decomposition_rate
mineral soil
soil_horizon
16S rRNA
target_gene
V4
target_subfragment
sandy loam
texture
physical analysis hydrometer
texture_meth
19UW-WB-09-09-A-pb-cDNA
title
1.25%
tot_carbon
0.10%
tot_nitro