SAMN03288813
External Id
Academia Sinica
INSDC center name
2015-01-20T00:00:00Z
INSDC first public
2022-12-19T21:05:44.921Z
INSDC last update
SRS823853
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS823853
SRA accession
A single colony from L. kluyveri (CBS 3082) was evolved by a long-term modified serial dilution transfer method (batch cultures) method in which bacteria were sequentially introduced to compete with yeasts under aerobic conditions. Both evolved and the ancestral strains were grown in duplicates in batch cultivation with synthetic minimal media. Batch cultures of evolved and ancestor were sampled for RNA extraction under both aerobic, post-diauxic and anaerobic conditions in fermeters to determine changes in expression of genes responsible for carbon utilization in the evolved strain. RNA was extracted from these samples. The cDNA libraries were constructed. Paired-end sequencing was performed on Illumina HiSeq2000.
description
Sweden: Lund
geo loc name
intestinal canal of Drosophila pinicola
host
Lachancea kluyveri
http://purl.obolibrary.org/obo/NCBITaxon_4934
organism
Cell culture
http://purl.obolibrary.org/obo/BTO_0000214
sample type
CBS 3082
strain
Lachancea kluyveri transcriptome of ancestor and evolved strain in aerobic, post-diauxic and anerobic conditions
title
type material of Saccharomyces kluyveri
type material