characteristics.text: SAMD00112399
characteristics.characteristic: External Id
characteristics.text: College of Bioresource Sciences, Nihon University
characteristics.characteristic: INSDC center name
characteristics.text: 2019-11-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-05T05:07:18Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS.ba.wastewater
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.ba.wastewater.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: free living
characteristics.characteristic: biotic relationship
characteristics.text: 2017-01-06
characteristics.characteristic: collection date
characteristics.text: NBRC:112778
characteristics.characteristic: culture collection
characteristics.text: Keywords: GSC:MIxS;MIGS:6.0
characteristics.characteristic: description
characteristics.text: freshwater biome
characteristics.characteristic: env_broad_scale
characteristics.text: methane fermentation tank
characteristics.characteristic: env_local_scale
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: env_medium
characteristics.text: 4500000
characteristics.characteristic: estimated size
characteristics.text: Japan:Fukushima, Date
characteristics.characteristic: geo loc name
characteristics.text: not provided
characteristics.characteristic: isol_growth_condt
characteristics.text: 37.76125 N 140.57864 E
characteristics.characteristic: lat lon
characteristics.text: lbkm
characteristics.characteristic: locus_tag_prefix
characteristics.text: 1
characteristics.characteristic: num_replicons
characteristics.text: Lachnospiraceae bacterium KM106-2
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2093742
characteristics.characteristic: organism
characteristics.text: New bacteria whole genome sequencing, isolated from methane fermentator
characteristics.characteristic: project name
characteristics.text: not provided
characteristics.characteristic: ref_biomaterial
characteristics.text: This bacterium was originally isolated from a mesophilic methanogenic bioreactor digesting sweet potato. Methanogenic sludge was inoculated onto an agar plate of RCM (reinforced clostridial medium) containing Red Starch as an indicator for amylase (grams per liter): yeast extract, 13; peptone, 10; glucose, 5; Red Starch, 1; NaCl, 5; sodium acetate, 3, Cysteine-HCl 0.5 (pH6.8) and anaerobically incubated at 37 degrees C for three days using AnaeroPack pouch. Colonies forming clear halo were isolated and purified by successive dilution and cultivation in the same condition. For pure cultivation, RCM containing 0.1 % normal soluble starch was used.
characteristics.characteristic: samp mat process
characteristics.text: Lachnospiraceae bacterium NBRC 112778
characteristics.characteristic: sample name
characteristics.text: KM106-2
characteristics.characteristic: strain
characteristics.text: Genome sequencing sample of Lachnospiraceae bacterium NBRC 112778
characteristics.characteristic: title
characteristics.text: heterotroph
characteristics.characteristic: trophic_level