characteristics.text: SAMN11070719
characteristics.characteristic: External Id
characteristics.text: Hospital del Mar Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2019-03-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-19T21:06:25.040Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS4458801
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS4458801
characteristics.characteristic: SRA accession
characteristics.text: 12m
characteristics.characteristic: altitude
characteristics.text: Verstrepen Laboratory collection (VIB)
characteristics.characteristic: biomaterial provider
characteristics.text: 2015-01-29
characteristics.characteristic: collection date
characteristics.text: We isolated an isogenic population of Lachancea kluyveri from streaked plates, grew a culture overnight in a culture tube, then inoculated cells in 20mL of a custom rich medium in 50mL Erlenmeyer flasks at 30C. 1.5ml of cell culture was harvested in log growth phase at an OD600 of approximately 0.25; 30 minutes prior to harvesting the cells, diluted hydrogen peroxide was added to a final concentration of 1.5mM. Subsequently we centrifuged and froze the cells at -80C.
characteristics.characteristic: description
characteristics.text: Spain: Barcelona
characteristics.characteristic: geo loc name
characteristics.text: Lab strain
characteristics.characteristic: isolation source
characteristics.text: Lachancea kluyveri
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4934
characteristics.characteristic: organism
characteristics.text: 1.5ml of cell culture at OD600 of 0.289
characteristics.characteristic: samp_size
characteristics.text: cell culture
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000214
characteristics.characteristic: sample type
characteristics.text: CBS3082
characteristics.characteristic: strain
characteristics.text: 30
characteristics.characteristic: temp
characteristics.text: L. kluyveri oxidative stress
characteristics.characteristic: title
characteristics.text: type material of Saccharomyces kluyveri
characteristics.characteristic: type material