characteristics.text: SAMN13531410
characteristics.characteristic: External Id
characteristics.text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-02T06:43:16.703Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-02T06:43:16.703Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS5817706
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS5817706
characteristics.characteristic: SRA accession
characteristics.text: 30 days
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000033
characteristics.characteristic: age
characteristics.text: ICMR 1152
characteristics.characteristic: cultivar
characteristics.text: Pearl millet (Pennisetum glaucum (L.) R. Br.) is an important crop capable to grow in harsh and marginal environments with the highest degree of tolerance to drought and heat stresses among cereals. Diverse germplasm of pearl millet shows significant phenotypic variation in response to abiotic stresses. This characteristic feature makes pearl millet a unique model among other cereals to study the mechanisms responsible for mitigating diverse abiotic stress. The present study focused on identifying the physiological response of two pearl millet high-resolution cross (HRC) genotypes i.e. Vapour pressure deficit (VPD) insensitive genotype (ICMR 1122) and VPD sensitive genotype (ICMR 1152), in response to low and high VPD. ICMR 1152 was found to be a lower transpiration rate (Tr) under high VPD conditions as it showed higher transpiration efficiency and lower root exudation as compared to ICMR 1122. Further, low and high VPD condition and genotype-specific Pg-miRs were identified by deep sequencing analysis. A total of 94, 23 known Pg-miRs are 53, 5 novel miRNAs were identified for ICMR 1152 and 1122 genotypes respectively. The Pg-miR165, 168, 170, and 319 family exhibited significant differential expression under low and high VPD conditions in both genotypes. Pg-miRs specific to adventitious roots development and various stress response has been deciphered. Suitable targets for each Pg-miRs were identified and validated the expression of top targets in both genotypes responsible for stress. This study shed light upon the role of miRNAs in the regulation of drought tolerance in pearl millet.
characteristics.characteristic: description
characteristics.text: root
characteristics.characteristic: dev stage
characteristics.text: Hyderabad
characteristics.characteristic: ecotype
characteristics.text: India: Hyderabad
characteristics.characteristic: geo loc name
characteristics.text: Cenchrus americanus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4543
characteristics.characteristic: organism
characteristics.text: Root
characteristics.characteristic: tissue
characteristics.text: Cenchrus americanus (genotype ICMR 1152) plants treated with high VPD conditions
characteristics.characteristic: title
characteristics.text: High VPD stress
characteristics.characteristic: treatment