characteristics.text: SAMN16878711
characteristics.characteristic: External Id
characteristics.text: The Australian National University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-08T05:42:02.287Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS7758491
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS7758491
characteristics.characteristic: SRA accession
characteristics.text: 2015/2017
characteristics.characteristic: collection date
characteristics.text: Stomach content samples requested from the Pacific Marine Specimen Bank (www.spc.int).
characteristics.characteristic: description
characteristics.text: Pacific Ocean
characteristics.characteristic: geo loc name
characteristics.text: Katsuwonus pelamis & Thunnus albacares
characteristics.characteristic: host
characteristics.text: Stomach content of tuna species captured in the Pacific ocean
characteristics.characteristic: isolation source
characteristics.text: gut metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_749906
characteristics.characteristic: organism
characteristics.text: www.spc.int
characteristics.characteristic: ref_biomaterial
characteristics.text: Amplicons obtained from the prokaryote primers.
characteristics.characteristic: samp mat process
characteristics.text: Each stomach was taken out of the freezer, defrosted and cut open using a longitudinal incision. Then, undigested carcasses and hard parts were removed and placed aside in a sterile container. The stomach lining was then scraped using the unsharpened side of a sterile scalpel and carefully placed inside a sterile 50 mL falcon tube. Following, the stomach lining was briefly washed with MilliQ water using a squirt bottle and mixed with the stomach gastric juices. Approximately 5-25 mL of stomach liquid was then collected using a 1 mL disposable pipette and placed inside the same 50 mL falcon tube as the lining scraped sample.
characteristics.characteristic: samp_collect_device
characteristics.text: 55 skipjack (Katsuwonus pelamis) and 41 yellowfin (Thunnus albacares)
characteristics.characteristic: samp_size
characteristics.text: Prokaryote
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007874
characteristics.characteristic: title