characteristics.text: SAMN16392823
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-03T11:33:02.252Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS9991554
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS9991554
characteristics.characteristic: SRA accession
characteristics.text: fetus
characteristics.characteristic: age
characteristics.text: ATCC CRL-1573
characteristics.characteristic: biomaterial provider
characteristics.text: HEK 293 cells were maintained by the procedures of Bulanenkova et al., 2007. T25 flasks with approximately 10^6 HEK 293 cells were transfected using Lipofectamine 3000 (Invitrogen, cat#L3000015, Carlsbad, CA, USA) according to manufacturer's recommendations. Briefly, immediately before transfection, media was changed on the flask. Lipofectamine:DNA complexes for the pCI null vector (Promega, cat#E1731, Madison, WI, USA) were added to the media with 6.5ug DNA administered. Lipofectamine:DNA complexes were prepared in OptiMEM medium (Gibco, cat#31985062, Gaithersburg, MD, USA), then added to HEK growth media (RPMI 1640:DMEM:F-12 (2:1:1) medium supplemented with 10% fetal bovine serum [Gibco: RPMI-1640: cat#11875093; DMEM/F12: cat#11320033; FBS: cat#A3160402]) currently in the flask. Following transfection, flasks were returned to incubate at 37 C /5% CO2. At 48 hrs post infection, the transfection medium was replaced with fresh HEK growth medium in each flask. At 72 hrs post infection, flasks were harvested by scraping cells in PBS, and DNA was isolated by a Wizard Genomic DNA Purification Kit (Promega, cat#A1120).
characteristics.characteristic: description
characteristics.text: ATCC CRL-1573
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: embryonic kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002120
characteristics.characteristic: tissue
characteristics.text: HEK 293 with transfection of an empty pCI plasmid (HEK-pCI)
characteristics.characteristic: title