characteristics.text: SAMN16387683
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-03T11:33:01.908Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS7708573
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS7708573
characteristics.characteristic: SRA accession
characteristics.text: Briefly, A. thaliana seeds were surface sterilized with 75% ethanol, plated on half strength Murashige and Skoog (MS) agar medium at 4 C in the dark for 3 days until uniform germination, and then were germinated on at 22 C in constant light for 10 days. The seedlings with about similar size were transferred to potting mix for growth in 22 C growth chambers with 16-h light/8-h dark cycles. The plant materials of whole seedlings were collected at day 21 after initiation of germination. Genomic DNA was extracted using a DNeasy Plant Mini kit (Qiagen) following manufacturers' instructions.
characteristics.characteristic: description
characteristics.text: 3 week seedlings
characteristics.characteristic: dev stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: USA
characteristics.characteristic: geo loc name
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: seedlings
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: tissue
characteristics.text: Arabidopsis thaliana 3 week seedlings
characteristics.characteristic: title