characteristics.text: SAMN16392390
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-03T11:33:01.683Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS10022181
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS10022181
characteristics.characteristic: SRA accession
characteristics.text: fetus
characteristics.characteristic: age
characteristics.text: ATCC CRL-1573
characteristics.characteristic: biomaterial provider
characteristics.text: HEK293 WGA was performed with the REPLI-g mini kit (Qiagen, cat#150023), following the manufacturer's instructions. Briefly, 5ng of genomic DNA was used in the reaction and incubated at 30 C for 16 hours, then heat-inactivated at 65 C for 3min. All WGA samples were purified using 0.8X Ampure XP beads (Beckman Coulter, cat#A63881, Indianapolis, IN, USA). The DNA concentration of the product was determined with the Qubit dsDNA High Sensitivity Assay kit (Life Technologies, cat#Q32854, Carlsbad, CA, USA).
characteristics.characteristic: description
characteristics.text: ATCC CRL-1573
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: embryonic kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002120
characteristics.characteristic: tissue
characteristics.text: HEK293 Whole Genome Amplification DNA
characteristics.characteristic: title