characteristics.text: SAMN16387687
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-03T11:33:02.083Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS7698901
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Invertebrate
characteristics.characteristic: NCBI submission model
characteristics.text: Invertebrate.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS7698901
characteristics.characteristic: SRA accession
characteristics.text: 45min
characteristics.characteristic: age
characteristics.text: 2020-05
characteristics.characteristic: collection date
characteristics.text: Fly stocks were maintained under standard culture conditions, and the w1118 strain was used for collect embryos. For optimal egg collection, healthy young male and female flies were crossed and maintained in tubes with new food. Prior to egg collection, files were fed with yeast paste on fresh grape juice agar plate for at least 2 hours. The 45 minutes embryos were then collected on grape juice agar plate and rinsed with water on a fine nylon mesh filter. To isolate the gDNA, the embryos were transferred into 1.5ml tubes and treated with lysis buffer containing 100mM Tris-HCl (pH 8.0), 5mM EDTA (pH 8.0), 0.2% SDS, 200mM NaCl, and 20-50 uL of protease K (20mg/ml) was added to the fly embryos, homogenized and incubated at 55 C overnight. After the overnight digestion, the lysates were brought to room temperature, and incubated with 5-10 uL of RNase A solution (20mg/ml) for at least 1 hour at 37 C. DNA was extracted by adding equal volume of phenol: chloroform: isoamyl alcohol at a ratio of 25:24:1 and centrifuging at 12,000 rpm for 5 min. Supernatant was transferred to clean tubes and mixed with 10% of 3M NaOAC and two times of 100% Ethanol at -80 C for at least 30 min. The gDNA pellets were collected by 12,000 rpm centrifugation for 15 min. After washing the gDNA pellet with 70% ethanol, the pellets were dissolved in 30-50 uL of nuclease-free water.
characteristics.characteristic: description
characteristics.text: USA
characteristics.characteristic: geo loc name
characteristics.text: w1118
characteristics.characteristic: isolate
characteristics.text: Laboratory
characteristics.characteristic: isolation source
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: embryo
characteristics.characteristic: tissue
characteristics.text: Drosophila melanogaster 45min embryo
characteristics.characteristic: title