characteristics.text: SAMN16392339
characteristics.characteristic: External Id
characteristics.text: Icahn School of Medicine at Mount Sinai
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-03T11:33:02.103Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS10021873
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS10021873
characteristics.characteristic: SRA accession
characteristics.text: fetus
characteristics.characteristic: age
characteristics.text: ATCC CRL-1573
characteristics.characteristic: biomaterial provider
characteristics.text: Human Embryonic Kidney (HEK)293 cells were obtained from ATCC (cat#CRL-1573). All media and tissue culture supplies were obtained through ThermoFisher Scientific. The cell growth protocol was based upon Bulanenkova et al., 2007. Cells were grown under sterile conditions in a 2:1:1 mixture of RPMI-1640 and DMEM:F12 and (supplemented at 10% with Fetal Bovine Serum (FBS)), splitting as needed based on confluence (~75%) and on media color. Cells were grown as an adherent culture in Nalgene Nunc tissue culture-treated flasks in a 37 C/5% CO2 incubator. Cells screened as negative for mycoplasma during culture, by testing with a MycoAlert Mycoplasma Detection Kit (Lonza, Inc, Morristown, NJ, USA). Cells were harvested at <25 passages with 0.25% trypsin, and gDNA was isolated using a DNeasy Blood & Tissue Kits (Qiagen) by the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: ATCC CRL-1573
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: embryonic kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002120
characteristics.characteristic: tissue
characteristics.text: Native HEK293
characteristics.characteristic: title